FL-MFCS Microfluidics Control System
Contents
1. FL MFCS Microfluidics Control System INSTRUCTION MANUAL Serial No 011211 World Precision Instruments MicroFluidics Control System WORLD PRECISION INSTRUMENTS MicroFluidics Control System CONTENTS ABOUT TES MAN aaa on fa ag i a a aaa langa aa d ia ta a 1 INTRODUCTHO N i sa nai iata oa aa a aa an a 2 ci atacau aa oale tai apa I 3 EE 4 INSTRUMENTE DESCRIPTION a 5 FRM INU CT 5 Ce at la za ial dal t ada a ct i balada afl aa 6 ERLE EE Lal aaa ial a o una Al alai ela ial daia a 12 GPERADNGHNS TROS TION Santander a ta ca dei 14 PRI SII eco ei ag amp taia ii 14 CONUL NE Pressure IN AS Channels aceeiasi iata cote es ia ata aaa daden 15 Jab Ge gil SUE 0a az aa i 16 MRS 16 COUPE cn vene bet ae a a nat aa al ali A a ae o sit canto 17 Manda VAWO MOIES n cacat za aaa tt aud e daf ti a stai Aa a i al la c 18 zielen COE RICO gl Penne mat paid aia ananas i ed 18 MEES Mat OCIS OTR IE ao 19 General DESSE ON ardennen dieet 19 DESCAPHOREOE IS WAG sanerering opveert na aaa eat 20 DESEHPUONS of LAS AGIO heap 20 TT Eg E a ag ca ani ta a a aa ala aaa al a 20 NW ilaria 21 GA ERRE 21 LAbVIOWE naar 24 COMM 24 aO PRO e l ea tac dia t ia tatal 25 Set Pressure and AlpNa Andloe DISMAY wensten neten 25 Set Pressure Digtal BIS IOU vangers amice area ye entre d aaa deal 25 A PPR RR PORN II ROI DR ene PANA i AR POPOR SA PI RR O E TA 26 Function Description preliminary nennen enensenensenensenensenensenenneneenenee2. WORLD PRECISION INSTRUMENTS 25 MicroFluidics Control System C and VBA Function Description preliminary Here is a global description of the functions provided with the library mfcs_initialisation Initialize the USB for the MFCS If the serial number is not specified or equal to 0 the first MFCS device found will be initialized If the serial Number is specified the MFCS device with the designated serial number is initialized Each MFCS has a unique serial number inscribed on the back panel The vi returns the handle and a boolean that confirms that the USB initialized correctly Close the MFCS device and the allocated the memory mfcs_set_purge_ Connect the channel 1 output directly to the pressure supply This on function should be used with care as it can cause damage to the microsystems connected mfcs_set_purge_ Disconnect the channel 1 output from the pressure supply Channel off 1 can be used normally mfcs get purge Get the status of the purge e TRUE purge ON e FALSE purge OFF mfcs_get_status Get the status of the MFCS e 1 Trouble in the MFCS connections USB or electric aperature O MFCS is reset MFCS needs to be manually rearmed green switch on the front panel 1 Normal 2 Pressure Supply Overpressure 3 MFCS needs to be manually rearmed after overpressure green switch on the front panel Get the characteristics of the specified channel Pressure Units 0 no captor 1 inches of
3. noms as D As She Dia 7 du wy Mai DA ra rea ee ume cad TA TETE eer and a a a wu teren pi een Te sro Arre wa Fig 42 Anti parallel coupling allows changes in the pressure difference of two channels without changing the pressure sum Parallel coupling keeps the pressure difference constant Keyboard Shortcut It is possible to temporally disable the coupling feature of a set of channels by pressing the shift key Then move the handles independently Manual Auto Modes The manual mode provides a proportional control on the valves The handles directly control the valves opening and no regulation occurs This mode is only adapted for special use and it is subject to intrinsic nonlinear characteristics hysteresis and limited reproducibility of the solenoid valves The auto mode provides a direct control on the pressure inside the channels The handles set the requested pressure and the embedded regulation program computes the command to give to the valves in order to have a stable quick and reproducible response Feedback Coefficient 400 5 450 400 350 4 400 360 4 300 4 360 L 300 250 4 300 250 4 250 200 J 200 200 150 150 150 4 100 100 100 4 50 4 50 50 4 DI 0 0 s9 5 10 15 20 pra 5 10 15 20 5 Fig 43 In the first graph the feedback coefficient is too high In the second it is optimized In the third it is too low These graphs show the effect
4. 255 n This value is linked to the feedback coefficient for the pressure regulation default value 5 Auto Double x Send a pressure order in mBar to the specified channel a Yn programme Goto label String s Go to the line with the s string in the labeled column Manual Double x The channel is control manually without feedback control X is equivalent to the of aperture of the Electrovalve Purge off a Stop purge on channel 1 Double x The program waits for x secondes Script Example Here is an example of a script with the corresponding pressure chart eT apha et DE 9 lgotolabel stat This is an endless loop generating a 40 seconds period square pressure signal 20 WORLD PRECISION INSTRUMENTS MicroFluidics Control System Example of MFCS Mat script for channel 1 and 2 250 200 150 Channel 1 Channel 2 100 Pressure mBar 50 0 50 100 150 200 250 Time s Fig 45 The script above generates this output Labview The Labview drivers are in the provided CD The Labview library provided is embedded with a set of functions that allows you to control the MFCS in your own Labview program Here are a few examples of what you can achieve with this library Labivew VI Labview vi_ symbol mfcs_close vi Close the MFCS device and the Handle Da allocated memory Error Error out in mfcs_data_ adi Handie dit Get the
5. foregoing warranty shall be applicable only to the extent of the warranty furnished by such other manufacturer This warranty will not apply to appearance terms such as knobs handles dials or the like WPI makes no warranty of any kind express or implied or statutory including without limitation any warranties of merchantability and or fitness for a particular purpose WPI shall not be liable for any damages whether direct indirect special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product Claims and Returns Inspect all shipments upon receipt Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Concealed loss or damage should be reported at once to the carrier and an inspection requested All claims for shortage or damage must be made within 10 days after receipt of shipment Claims for lost shipments must be made within 30 days of invoice or other notification of shipment Please save damaged or pilfered cartons until claim settles In some instances photographic documentation may be required Some items are time sensitive WPI assumes no extended warranty or any liability for use beyond the date specified on the container e WPI cannot be held responsible for items damaged in shipment en route to us Please enclose
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7. mfcs mfcs_vb dlI ByVal H As Long As Byte Public Declare Function mfcs_set_purge_off Lib d mfcs mfcs_vb dIl ByVal H As Long As Byte Public Declare Function mfcs_get_purge Lib d mfcs mfcs_vb dIl ByVal H As Long ByRef C As Byte As Byte Public Declare Function mfcs_get_status Lib d mfcs mfcs_vb dll ByVal H As Long ByRef C As Byte As Byte Public Declare Function mfcs_read_chan Lib d mfcs mfcs_vb dIl ByVal H As Long ByVal Chan As Byte ByRef P As Single ByRef Chrono As Integer As Byte Public Declare Function mfcs_data_chan Lib d mfcs mfcs_vb dIl ByVal H As Long ByVal Chan As Byte ByRef Su As Byte ByRef Sm As Integer ByRef Zr As Integer ByRef Ms As Integer WORLD PRECISION INSTRUMENTS 29 MicroFluidics Control System ByRef Chrono As Integer As Byte Public Declare Function mfcs_get_serial Lib d mfcs mfcs_vb dll ByVal H As Long ByRef Serial As Integer As Byte Public Declare Function mfcs_set_auto Lib d mfcs mfcs_vb dIl ByVal H As Long ByVal Chan As Byte ByVal P As Single As Byte Public Declare Function mfcs_set_alpha Lib d mfcs mfcs_vb dll ByVal H As Long ByVal Chan As Byte ByVal Alpha As Byte As Byte Public Declare Function mfcs_set_manual Lib d mfcs mfcs_vb dll ByVal H As Long ByVal Chan As Byte ByVal Ev As Single As Byte Public Declare Function mfcs_set_zero Lib d mfcs mfcs_vb dll ByVal H As Long ByVal Chan As Byte ByVal Zero As Integer As Byte About Output Pressuriza
8. of the feed back coefficient value on the pressure stability and response time The MFCS Control software provides optimal pressure and flow regulation for a wide range of microfluidic applications in particular those involving extremely low pressures and or volume flow rates uL to pL min However when using large volumes an 18 WORLD PRECISION INSTRUMENTS MicroFluidics Control System adjustment of the feed back loop s reactivity can be necessary to achieve optimal results This can be achieved independently for each channel by adjusting the feed back coefficient The factory setting is 5 a smaller coefficient results in a slower reaction a higher value gives a faster response If the feedback coefficient is too high the regulation loop can become unstable and pressure control inside the channel is no longer possible The optimal response is obtained just below the critical damping situation between a monotonous return to equilibrium and oscillatory overshooting See Fig 43 NOTE For high pressure versions of the MFCS at the initial factory setting operation is oscillation free for small pressure changes but shows overshooting and transient oscillations for drastic pressure changes If this overshooting is a nuisance to your application it can be suppressed by decreasing the feedback coefficient You will lose some of the regulation speed for small pressure changes A working balance must be achieved The optimal
9. only necessary once a day Preheating procedure WARNING PREHEATING PROCEDURE Please switch on the GREEN button on the MFCS to start procedure and wait for 10 minutes Remaining time 10 00 Fig 18 When the green button is pressed the green LED illuminates left and the warning appears on the computer screen right 10 Unpack the Fluiwell optional accessory not included Verify that the four toric seals WORLD PRECISION INSTRUMENTS 9 MicroFluidics Control System are in place before mounting the reservoirs Fig 19 A toric seal is marked by the arrow in the image 11 Screw the 2mL reservoirs on the Fluiwell Fig 20 The reservoirs are in position 12 Prepare your experimental setup by gathering all the devices and elements you might need The length of the tubing corresponds with the distance between the Fluiwell and the MFCS Microfluidic Chip Fig 21 Position the system components as shown in the image 13 The connectors that are included with the system are 0 0625 If you prefer to use 10 WORLD PRECISION INSTRUMENTS MicroFluidics Control System different connectors see Appendix A for a list of suppliers You can also find suppliers for the tubing in the appendix You will need four pieces of soft tubing and four pies of FEP tubing For the MFCS 8C you will need eight pieces of each vr Fig 22 The included connectors are installed left The image right
10. sent from the computer to the MFCS order 7 MECS S N see Mien comprising serial and version number purge is switched on mfcs reset detected system has realized activation of reset signal button processor reset line Fig 57 Commands sent from the MFCS to the computer WORLD PRECISION INSTRUMENTS 39 MicroFluidics Control System WARRANTY WPI World Precision Instruments Inc warrants to the original purchaser that this equipment including its components and parts shall be free from defects in material and workmanship for a period of one year from the date of receipt WPI s obligation under this warranty shall be limited to repair or replacement at WPI s option of the equipment or defective components or parts upon receipt thereof f 0 b WPI Sarasota Florida U S A Return of a repaired instrument shall be f o b Sarasota The above warranty is contingent upon normal usage and does not cover products which have been modified without WPI s approval or which have been subjected to unusual physical or electrical stress or on which the original identification marks have been removed or altered The above warranty will not apply if adjustment repair or parts replacement is required because of accident neglect misuse failure of electric power air conditioning humidity control or causes other than normal and ordinary usage To the extent that any of its equipment is furnished by a manufacturer other than WPI the
11. shows the soft tubing on the top and the FEP tubing on the bottom 14 Connect the soft tubing to the Fluiwell Fig 23 The soft tubing is connected 15 Connect the FEP tubing to the top outlets Screw the fitting down finger tight in order to avoid leaks The FEP tubing should be visible inside the 2mL reservoirs Fig 24 The FEP tubing extends into the 2mL reservoirs 16 Your Fluiwell is ready to connect with your microfluidic chip and the MFCS Before making the connections fill the 2mL reservoirs with your sample WORLD PRECISION INSTRUMENTS 11 MicroFluidics Control System Starting the MFCS 1 Remove the protection caps from the MFCS outlets Use the provided wrench or any Yq tool F Free Outlet Fig 25 Remove the caps from the outlets 2 Tightly screw the soft tube adapters on the outlets Fig 26 Soft tube adapter are screwed into the outlets 3 Connect the soft tubing to the MFCS outlets Fig 27 The soft tubing from the Fluiwell is connected to the soft tube adapters on the MFCS outlets 12 WORLD PRECISION INSTRUMENTS MicroFluidics Control System 4 Connect the Fluiwell to your microfluidic chip using the FEP tubes A list of adapters for smaller inner diameters tubes is available in the appendix Fig 28 Connect the Fluiwell to the microfluidic chip 5 Fill the 2mL reservoirs with the appropriate fluid corresponding to the inlets of your chip 6 Atthe end of
12. the preheating countdown e Push the red button e On the computer interface calibrate the MFCS by selecting Setup gt Compensate offset Click the Save to MFCS button to load your calibration parameters into the MFCS and close the window In order to guarantee the accuracy of your measurements we recommend calibrating the device every time you move it Otherwise once a month is sufficient The MFCS is ready to be used File Setup Help Compensate offset E oY Purge warning Fig 29 MFCS Setup menu Offset compensation MFCS Last Average value value value Chan 1 417 417 47 Chan2 4 40 420 Chan3 416 5 6 Chan4 47 8 418 Fig 30 Offset Compensation Window Save to MFCS Fig 31 Save to MFCS button WORLD PRECISION INSTRUMENTS 15 MicroFluidics Control System OPERATING INSTRUCTIONS Here you will learn how to operate the MFCS using the MFCS control interface AT Pressure Preheating ET Ren Handle Warning Pr vare mana a TT TT TTT Sd FLUIGENT vane emme conte Pressure scroll Direct Coupling field Button MFCS Requested Status a Pressure field Purge ed Button _b k Config Tabs nn L Ler rents and Setting stia TAO LAI retin mt RACE Die LoS oF Fig 32 MFCS software control interface Purging 1 Click on the Purge button A warning appears asking for confirmation Click Yes Warning Do you really want to purge on channel 1 7 Do not ask this qu
13. value depends on several features of the connected system including volume and elasticity of tubing hydrodynamic resistance and geometry of the microfluidic channels and the time constant of the phenomena under investigation MFCS Mat Scripting Software General Description Pe Poow eb Kang Laet Luctor jol la Une Camas i sate van as m Chart FLUIGENT La Table here it is blank NR a ai A p MFCS Status SSS e 2 3 2 i m omu Ka mm r Pap On Fig 44 MFCS Automat window MFCS mat is a script software that allows you to create automated pattern of pressure In the main window Fig 44 you can find e A chart showing the pressure value of all the channels here it is blank e Below the chart the MFCS status is shown with the following buttons Play Pause Stop and Verify program e On the right hand side you can add different actions in the table script WORLD PRECISION INSTRUMENTS 19 MicroFluidics Control System Description of the Table In this table there are six columns e Label needs a string It is where the action Goto label looks for e Action define the type of action e Ch define the channel number where the action is applied e Parameter define the parameter of the action if necessary e Unit specify the unit of the parameter automatically set e Comment define a comment of the current action Descriptions of the Actions Alpha Integer from O to
14. water 2 PSI Maximum Pressure range in the specified units Zero pressure value of the captor unsigned 12 bits Pressure value unsigned 12 bits Chrono value unsigned 16 bits mfcs_get_serial Get the Serial Number of the MFCS 26 WORLD PRECISION INSTRUMENTS MicroFluidics Control System mfcs set auto Set the regulated pressure mBar for the specified channel from 1 to 4 or 8 If the specified channel is 0 the same is applied to all channels mfcs_set_alpha Set the alpha value default value 5 for the specified channel from 1 to 4 or 8 If the specified channel is 0 the same is applied to all channels Alpha is linked to the proportional value of the PID mfcs_set_manual Set the electrovalve aperature for the specified channel from 1 to 4 or 8 If the specified channel is 0 the same is applied to all channels The manual control of the electrovalve is not recommended and the output pressure is no longer regulated Save in the EEPROM the zero pressure value of the specified channel The following array gives an algorithmic description of the previous functions function return parameter comment mfcs_ UL handle US serial number Initialise USB connection and initialisation look for a MFCS with the specified serial number handle 0 if no USB connection UL handle Close USB connection mfcs_read c error w A string on the interface mfcs_write c error tige a string on the interface mfcs_se
15. 2 R3 joining in one point with pressure pc we find using the continuity equation the sum over all Qs going into one knot vanishes P3 Pi P Ps _RiPoRs R R Ral R R R 1 2 3 1 2 3 Q Q gt Pi P3 Pe Po7 The corresponding flow in channel 1 as an example can be obtained with Ohm s law Q1 p1 pc R1 The other values are equivalent To obtain the corresponding formulas for n channels joining in one knot the latter formula can be generalized n Pi gt i DR More complex geometries can be calculated using well known methods in analogy to electrical resistor networks pressure is equivalent to the electric potential flow is equivalent to the current and flow resistance of a channel plays the same role as the Ohm s resistance WORLD PRECISION INSTRUMENTS 37 MicroFluidics Control System Trace and Log Flle Formats MFCScontrol allows a complete real time recording of all settings and measured pressure values during operation 11 07 2005 17 39 03 MFCS S N 0003 V 000 011 15885 15886 15887 15888 15889 15890 15891 15892 18 424 18 424 18 386 18 371 18 485 18 462 18 402 18 333 0 426 0 426 0 395 0 426 0 395 0 426 0 426 0 395 5 146 5 100 5 100 5 123 5 100 5 100 5 146 5 123 20 560 20 477 20 454 20 583 20 583 20 500 20 500 Fig 54 Example of a trace file The trace file format consists of a header line and a data block The header line is composed of the starting date
16. CS4C e ECS MAAT AC MFCS user MFCS_MAT 4C i d Visual Basic drivers Fig 13 Double click on the MFCS4C to install the MFCS4 5 Turn the MFCS ON using the switch on the rear panel The red light illuminates to indicate that the power is on If not check the power supply and the USB cable Fig 14 The red LED illuminates to indicate the power is on 6 Double click on the MFCS control icon MPS user manual pdf Fig 15 The MFCS icon is highlighted in the image 7 The software opens and is ready for use 8 WORLD PRECISION INSTRUMENTS MicroFluidics Control System Pile TD Chan 1 Chan Chand Chana Pena mk Po oe Teme LW Pd laan ar Pe WEL ne mari pana mrri sani im Dl mara arn IC Cazarma darei Berlinde TAC SAE ra AC e Li nt Fig 16 MFCS software welcome screen 8 The pressure source must be CLEAN AND DRY and fixed to the input pressure value specified on the rear panel of your MFCS It is located next to the input pressure valve of the MFCS Fig 17 The label indicates the correct input pressure for your system Pressure Range mBan 25 oo 25 1000 25 09 9 Press the green button and set the pressure source to the input pressure value of your MFCS The preheating will start A preheating procedure is necessary to warm up the MFCS 10 min Meanwhile you can continue with the next step Avoiding this step can decrease the pressure control stability and accuracy Preheating is
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18. and time serial number of the connected unit and version number of the MFCScontrol software The data block is composed of lines carrying a time stamp absolute time and the internal step number and the 4 8 pressure values N A means information was lost for example transient transmission failure When using this option avoid the creation of oversized data files Adjust the storage frequency as necessary See the pull down menus 11 07 2005 17 27 38 BEGIN OF LOG 11 07 2005 17 27 43 AUTO Chan 1 2196 11 07 2005 17 27 43 AUTO Chan 1 2221 11 07 2005 17 27 43 AUTO Chan 1 2246 11 07 2005 17 27 43 AUTO Chan 1 2283 11 07 2005 17 27 43 AUTO Chan 1 2332 11 07 2005 17 27 43 AUTO Chan 1 2381 11 07 2005 17 27 43 AUTO Chan 1 2406 11 07 2005 17 27 45 AUTO Chan 1 2715 11 07 2005 17 27 45 AUTO Chan 1 2727 11 07 2005 17 27 46 AUTO Chan 1 2739 11 07 2005 17 27 46 AUTO Chan 1 2739 11 07 2005 17 27 46 AUTO Chan 1 2739 11 07 2005 17 39 27 END OF LOG Fig 55 Example of a Log file command Joor O SSS S S ALPHA Chan X YYYYY set X value to YYYYY in Channel X AUTO Chan X YYYYY set value YYYYY in Channel X in feed back mode BEGIN OF LOG start logging END OF LOG stop logging 38 WORLD PRECISION INSTRUMENTS MicroFluidics Control System command Joor O SS SSS SS MANUAL Chan X YYYYY set value YYYYY in Channel X in manual mode PURGE OFF open purge valve Fig 56 Commands
19. characteristics of the chan vi Sanie dat Channel data specified channel Error in ali iau Pressure unity O no sensor 1 water inch 2 PSI Maximum pressure range in the specified unity Zero pressure value of the sensor unsigned 12 bits Pressure value unsigned 12 bits WORLD PRECISION INSTRUMENTS 21 MicroFluidics Control System Labview vi_ symbol mfcs_get_ purge vi mfcs_get_ serial vi mfcs_get_ status vi mfcs_ initialisation VI mfcs_read_ chan vi 22 Handle in Enora Get status of the purge in channel Enor Purge ON 1 in Handle out True Purge ON False Purge OFF Handle in Handle out Get the serial number of the MFCS Ed Sora Number 0 if no MFCS connected Error i Error out Handle in Handle out Get the status of the MFCS Lee mes Status O MFCS is reset MFCS needs nee to be manually rearmed switch on GREEN button 1 Normal 2 Pressure Supply Overpressure 3 MFCS needs to be manually rearmed alter overpressure Switch on GREEN button Handle in Handie out Initialize the MFCS device EA o Serial number e Ifthe serial number is not in nee specified or equal to 0 the first MFCS device found will be initialized If the serial number is specified the corresponding MFCS device is initialized Each MFCS has a unique serial number written on the back panel The VI returns the handle and the serial number of the MFCS 0 if no MFCS found Handle i
20. d and the output pressure is no longer regulated Disable purge feature Channel 1 can be used normally Enable purge feature Channel 1 is directly connected to the pressure supply Please use with care in order to avoid any damage to your microsystem Calibrate the zero pressure value of the specified channel This value can be estimated with mfcs_data_ chan vi 23 MicroFluidics Control System Labview Examples MFCS 8C control w n mare hiw a are ave awd s i 4 ver zel mars mm 6s rara ere wera rrea sra rei tere m ha mnd ave n oe Lares barre race ere tore Fig 46 This VI is a transposition of MFCS control It integrates MFCS control softwares functions in a Labview environment 1 Enter the serial number Calibrate the MFCS if needed Set Pressure Order and Alpha feedback coefficient in the channels of interests Press Purge channel 1 only to fill or empty your device Ae DN Press Stop to stop the pressure Control 2 MFCS INET LAG TATION Beurt OUTPUT _ cs Onerre trba Tretare eta Messe me ies 1 es 1 found lo Fig 47 This VI lets you control several MFCS at the same time 1 Enter the two serial numbers 2 Select the MFCS of interest with the 1 2 switch of the INPUT box 3 Setthe Channel number Pressure order and appropriate Alpha feedback coefficient 24 WORLD PRECISION INSTRUMENTS MicroFluidics Control Sys
21. default value is 5 and a preheating of the electro valves is necessary 45 aperature for 10 min Set electro valve voltage on the specified channel If the value is O it is set the same for all channels Save Zero sensor value on the firmware To get this value use mfcs_data_chan PUS mesure WORLD PRECISION INSTRUMENTS MicroFluidics Control System unsigned long U32 ByVal Long unsigned short U16 ByVal Integer char ab ByVal String ByVal Byte ByRef Byte ee char for boolean result 1 ByVal Byte true O false PB 32 pointer on boolean ____ U8 byaddress ByRefByte __ SGD ByVal Single pointer on float SGL by address ByRef Single NB pointer on unsigned short U16 by address ByRef Integer 1 in Visual Basic all values are signed 2 After calling mfcs_initialisation a delay 0 5s must be set before calling other functions 3 A mfcs_close must be called before leaving the application to avoid a Windows error VBA Declarations Examples Public Declare Function mfcs_ initialisation Lib d mfcs mfcs_vb dIl ByVal i As Integer As Long Public Declare Function mfcs_close Lib d mfcs mfcs_vb dll ByVal H As Long As Byte Public Declare Function mfcs_read Lib d mfcs mfcs_vb dll ByVal H As Long ByVal S As String As Byte Public Declare Function mfcs_write Lib d mfcs mfcs_vb dIl ByVal H As Long ByVal S As String As Byte Public Declare Function mfcs_set_purge_on Lib d
22. ed pressurized gas from MFCS Fig 8 Fluiwell s working principle Setup AN WARNING MFCS MUST BE USED IN A CLEAN AND DRY ENVIRONMENT l i WARNING NO LIQUID SHOULD ENTER THE DEVICE THIS VOIDS THE WARRANTY WARNING THE PRESSURE SOURCE MUST BE DRY DUST AND OIL FREE USE A 0 01um FILTER AND A PRESSURE REGULATOR TO INSURE PROPER INPUT PRESSURE 6 WORLD PRECISION INSTRUMENTS MicroFluidics Control System Fig 9 Setting up the MFCS with Fluiwell Please follow the next series of operation in order to set properly your MFCS 1 Unpack the MFCS NOTE If something is damaged email TechnicalSupport wpiinc com Fig 10 MECS unit 2 Make the following connections on the rear panel e USB cable e Power supply e Pressure source RUN CLEAN DRY GAS THROUGH A PRESSURE REGULATOR BEFORE CONNECTING THE PRESSURE SOURCE TO THE MFCS See Required Materials on page 5 Fig 11 Connections made to the back panel 3 Insert the MFCS software CD into the computer Copy the folder corresponding to WORLD PRECISION INSTRUMENTS 7 MicroFluidics Control System your device MFCS 4C for 4 channels and MFCS 8C for 8 channels folder to desired location on your hard drive zS A MFCS 4C MFCS 8C Fig 12 Folder icons shown on the CD 4 Open the folder you just copied on your hard drive Then double click on the soft ware folder that has the same name d C Drivers Labview drivers V1_01 a di MF
23. estion again Fig 33 Warning message NOTE A purge feature is available in channel 1 It applies maximum pressure on this channel It suits any filling or emptying purpose 2 To disable the purge click the Purge button again The Purge On green indicator ap pears until you stop the purge MFCS Status USB open Standby 9 Operating Exceeding input pressure Purge On Fig 34 SFCS Status window 14 WORLD PRECISION INSTRUMENTS MicroFluidics Control System Controlling Pressure in the Channels There are two different way to modify the pressure in a channel 1 Press the Direct Control button to activate the control of the MFCS Otherwise the orders will not be transmitted 2 Then choose one of the following options e Change the pressure in the channels using the corresponding handle Fig 35 Move the slider up to raise the pressure e Enter the numerical value of the pressure in the requested pressure field Maximum pressure mbar 75 258 Requested pressure mbar 40 417 Measured pressure mbar 40 459 z E J Feed back coefficient 5 or i Fig 36 Enter the desired pressure into the appropriate field to change pressure in the channels Keyboard Shortcuts e Up arrow Moves the handle up one step e Down arrow Moves the handle down one step e Page up Moves the handle up 10 steps e Page down Moves the handle down 10 steps e Home Moves the handle to the top of the scale e End Moves the
24. etter calibrated scale ZII Quick start Min output pressure sep 095 mbar Min Max C 900 __ Output pressure precision ler We Better if calibrated Quick start 02 a a ee ee en nn operation temperature range 5 25 o ce _ EE E E 0 CR Weight 1 9 2 25 Depends on E Power Power consumption a W W 34 WORLD PRECISION INSTRUMENTS MicroFluidics Control System APPENDIX A Response and Stabilization Time Here are the graphic definitions of response time and stabilization time Pressure ee i mj m nn mn ilizationTime 100 Response Time p Ti Fig 52 On this graph you can see when the system begins to respond and when it actually stabilizes Unit Conversion Table value inch H20 factor xunit 1 inch H O 0 249 2 49x10 3 3 612x10 2 Example 10 kPa 10 kPa x 0 01 bar kPa 0 1 bar WORLD PRECISION INSTRUMENTS 35 MicroFluidics Control System Hydrodynamic Resistance of Rectangular Channels and Networks Pressure difference Ap the volume flow rate Q or the averaged velocity u and the friction coefficient C are related with channel dimensions a and b when a b gt gt 1 channel length L and viscosity p u 9 5 x10 Pa s for H O at 25 C b Ca di L Ap abu b i L a The relation between the friction C and the aspect ratio a b can be calculated Stone Stroock Ajdari Ann Rev Fluid Mech 2004 cir y
25. handle to the bottom of the scale WORLD PRECISION INSTRUMENTS 15 MicroFluidics Control System Tab Configurations 1 You can use the config tabs to set different handle patterns in order to easily switch between several pressure sets by clicking on the corresponding tab Chan 1 Chan 2 Chan 3 Chan 4 o Fig 37 Use the configuration tabs along the bottom of the window to switch between several pressure sets An example is shown in the graph above 2 The curves will follow the pressure pattern See MFCS Mat Scripting Software on page 19 or Labview on page 21 for automated pattern setting Chan Chan 2 Chan 3 Chan 4 gt da a ma Fig 38 This sample shows a pressure pattern with no curve Saving Curves 1 To save a set of curves first choose the desired trace sampling rate Fig 39 Choose File gt Trace rate to select the desired sampling rate 16 WORLD PRECISION INSTRUMENTS MicroFluidics Control System 2 Choose File gt Save Trace to start recording Give a name to the curves file in Save Trace dialog box of the file menu A check mark appears next to the Save trace menu item Setup Help Setup Help Open Ctri 0 Open Ctrl 0 Save Ctrl 5 Save Ctrl 5 Save as Save as Save log da E N T Save log N T Trace rate b Cn B Fig 40 File menu with a check mark next to the Save Trace option indicates the system is recording and the menu without a check mark shows the system i
26. idics Control System INTRODUCTION To your application Fluiwell Reservoirs Fig 2 Connecting the MFCS system The MicroFluidic Control System MFCS is a high precision pneumatic pressure controller designed to handle fluid in microfluidics systems microchannels and nanochannels capillaries Lab on chips It allows a stable and pulsation free flow with short response times 100ms and a stabilization time as low as 1s With the MECS it is also possible to control several independent channels up to 16 cf Labiview VI simultaneously The user friendly software allows you to create scripts for complex flow patterns or dynamic coupling for user controlled dependence between channels Channels Pressure Range mBar 0 25 0 69 os 0 1000_ 4 Channel MFCS 4C 25 MFCS 4C 70 MFCS MFCS MFCS NEG MFCS NEG 4C 345 4C 1000 4C 25 4C 70 8 Channel MFCS 8C 25 MFCS 8C 70 MFCS MFCS MFCS NEG MFCS NEG 8C 345 8C 1000 8C 25 8C 70 Pressure P gt 0 P lt 0 type 2 WORLD PRECISION INSTRUMENTS MicroFluidics Control System Parts List After unpacking verify that there is no visible damage to the instrument Verify that all items are included 1 MFCS unit 1 power supply and a power line cable 1 USB cable 1 pneumatic connection kit 4 soft tube adapters 4x50cm of soft tube OD 3mm ID 1mm a Ya wrench For a 8 channel MFCS 8 adapters are included 1 Instruction Manual FEP Caps Fl
27. n Handle out Get the pressure value mBar E dla E EE me and the measure time ms of the easure Time ms in Error out specified channel from 1 to 4 or 8 WORLD PRECISION INSTRUMENTS MicroFluidics Control System Labview VI symbol Gharinel A AL Handle out default all Alpha default Error out mfcs_set_ alpha vi mfcs_set_ auto vi mfcs_set_ manual vi mfcs_set_ purge_off vi mfcs_set_ purge_on vi mfcs_set_ Zero vi WORLD PRECISION INSTRUMENTS Alpha default 5 Handle in Handle out ilie i i Uau B mBar mitul Handle in Handle out Channel Col Error out Handle in Ey Handle out Error Error out in Handle in Ei Handle out Error Error out in Handle in Handle out Channel number O all Zero sensor Error out Error in Set the alpha value default 5 for the specified channel from 1 to 4 or 8 e If the specified channel is O the same is applied to all channels Alpha is linked to the proportional value of the PID pressure regulation Set the regulated pressure value mBar for the specified channel from 1 to 4 or 8 e If the specified channel number is O the same pressure is applied to all the channels It is also possible to set the alpha value Set the electrovalve aperture for the specified channel from 1 to 4 or 8 e If the specified channel is O the same is applied to all channels The manual control of the electrovalve is not recommende
28. neneeneneenen 26 VBA Declarations AMP iaia 29 ADOUVOUPU Press Ze 0 ocoale N alai aa gena 30 MAINTENANCE aa za 31 ACCESSORI lla 32 SPEFICA NON einne o E T ina E aa ia a apc 33 MESS billion 33 MO 33 MPCS 3530 MB GL ici tatea based aa bea DD adapa ad 34 APPENDI A i pdl tt i oaia ie aia ala aaa n aa 35 Response and Stabilization ie 35 UE CONV E ION TROIS sa cea ca ea ai na a i a ta la all aa aa 35 Copyright 2011 by World Precision Instruments Inc All rights reserved No part of this publication may be reproduced or translated into any language in any form without prior written permission of World Precision Instruments Inc WORLD PRECISION INSTRUMENTS MicroFluidics Control System Hydrodynamic Resistance of Rectangular Channels and Networks 36 ace and Log Fe Poma 38 WARRANTY lla 40 clams and Returns RR RE IRI RR ION OR OR RR RR NI IDE NAANA AEA N II RA II RIP 40 WORLD PRECISION INSTRUMENTS MicroFluidics Control System ABOUT THIS MANUAL The following symbols are used in this guide This symbol indicates a CAUTION Cautions warn against actions that can cause damage to equipment Please read these carefully This symbol indicates a WARNING Warnings alert you to actions that can cause personal injury or pose a physical threat Please read these carefully NOTES and TIPS contain helpful information Fig 1 Flowell microfluidics system WORLD PRECISION INSTRUMENTS 1 MicroFlu
29. nput pressure when grren button on Front View Pressure outlet Green Button Red Button Fig 5 MFCS front and rear panels are labeled Required Materials The MFCS requires the following materials e A computer e A pneumatic pressure source e USB 1 1 port or faster Dry non corrosive use an air drier e Screen resolution of e Dust and oil free 1024x768 e Pressure regulator to tune the input e Intel Pentium II 500 MHz or pressure value faster e 0 01pm Filter e Windows 2000 Windows XP e Pressure range of the pump to suit e 5Mb of free disk space your MFCS input pressure Fig 6 Norgren Norgren F07 200 A3TG F72C 2GD QT0 Air drier Pump of Local Pressure Network Pressure Regulator Watson Smith LS6 2RT Gas Cylinder Only for positive pressure Fig 6 Suggested pressure source scheme WORLD PRECISION INSTRUMENTS 9 MicroFluidics Control System Pressure Range mBar_ 0 to 25 0 to 69 0 to 345 0 to 1000 25 to 0 69 to 0 PSI 0 35 _ 1 00 5 00 14 50 8 1300 7 25 00 Input Pressure mBar 500 500 800 value PSI 7 25 1725 1160 18 86 725 725 Fig 7 Input pressure value according to the MFCS pressure range Connection to the MFCS requires either e The Fluiwell a microfluidic interface between the MFCS and your Microdevice that uses 1 16 OD tube or smaller see Fig 8 e Your own connection system To microfluidic application gt Controll
30. s l 1 1 tanh BLE si 2 Pa with de 1 12 0 06 0 04 0 02 DI 1 10 100 x Fig 53 This graph shows the friction coefficient as a function of the aspect ratio of the channel and can be used to estimate the flow rate due to a given pressure difference Typical values are x 1 gt C 0 03512 x 2 gt C 0 05708 x 10 gt C 0 07688 Example A channel of 10x100um with a length of 10mm contains 0 01 ul Applying a pressure difference of 10Pa 100 uBar gives a volume flow rate of about Q 0 0005yl 36 WORLD PRECISION INSTRUMENTS MicroFluidics Control System min and an averaged flow velocity of u 5um min A pressure of 100 000 Pa 1 bar is 10 000 times higher thus the flow rate and velocity are also 10 000 times increased Since Ohm s law also applies to flow of other particles than electrons for example water we can transfer the formulas For a single channel we have for the volume flow Q as a function of the externally applied pressure difference p and the resistance R Q p R Q abu The pressure pc at an arbitrary position in a single channel with partial resistances R1 and R2 of the two halves of the channel is _ p Ro PR Q Q2 R R o n e The total resistance is R R1 R2 the flow yields Q p1 pc R1 R2 is equivalent If the two parts are of equal resistance equal length and diameter we find the well known average value pc p1 p2 2 For three channels p1 p2 p3 and RI R
31. s not recording 3 To end the recording choose File Save Trace again The check mark disappears The trace file is saved as a text file that can be imported into any spreadsheet The file looks similar to the example shown below Automat V 1 05 MFCS N 0005 V 000B 140 017 C oO o 0 105 VARE E Fig 41 The table shows the time of the experiment the time step n pressure of channel 1 pressure of channel 2 pressure of channel 3 and pressure of channel 4 In addition it has a line to indicate the start and one for the finish For more information on curve file formats see Trace and Log File Formats on page 38 Coupling Coupling is a feature that creates software driven dependence between channels Thus it is possible to increase pressure in one channel while the pressure in a second one is decreasing with the same variation Both parallel and anti parallel coupling are possible e To set a parallel coupling between channel 1 and channel 2 choose A for both channels in the coupling field e To achieve an anti parallel coupling just choose A for one of the two channels e To couple more than one channel set the coupling field of each channel WORLD PRECISION INSTRUMENTS 17 MicroFluidics Control System UC N ME peer ssss lt s S X it i me m mo me Chan Chan 2 Chan 3 Chan 4 Chan Chan 2 Chan J Chan 4 A Vate casi ene eae dFLUIGENT SI SA FLUIGENT m pn mage Meme mn Faa Came Caan T ha
32. t_purge_ C error UL aooo Open purge on channel 1 on mfcs_set_purge_ UL handle Close purge on channel 1 off mfcs_get_purge C error UL ULhande Get the purge state PB purge state mfcs_get_status c error UL handle Get MFCS status O if the MECS is reset 1 if normal PC Status 2 if overpressure 3 if MFCS needs to be rearmed WORLD PRECISION INSTRUMENTS 27 MicroFluidics Control System function return parameter comme UL handle C channel PF pressure mfcs read chan C error PUS chrono mfcs_data chan C error UL handle C channel mfcs_get serial UL handle PUS Serial mfcs set auto C error US handle mfcs set alpha C error UL handle C alpha mfcs set manual C error UL handle mfcs set zero C error UL handle Definition of C error e 0 OK e 1 USB closed e 2 Wrong channel 28 Read the pressure value mBar of the specified channel with the timing time unit 25ms Read the sensor data sensor unit 0 no sensor 1 H O 2 4908 mBar 2 psi 68 946 mBar full scale in pressure unit Zero value sensor U12 direct pressure measure U12 chrono time unit 25ms Get the serial number of the MFCS Regulate pressure mBar on the specified channel if O the same for all channel Set the alpha value U8 This value is linked to the PID performance The recommended value is 50 for MFCS devices sold with MFCS Control V1 05 For a more recent version V1 06 and V1 07 the
33. tem 4 Press the Stop button to stop pressure Calibration The Calibration process can also be in operated from Labview interface serial Number Number of channel Channel Data j19 A 4 Unity sensor Calibration sample size i guaio Max sensor Zero sensor 409 Measure sensor 1409 Chrono ms 1769075 9 Calibration po under process 100 Fig 48 Calibration controls Set Pressure and Alpha Analog Display This example allows you to set an order pressure and feedback coefficient and then to read the result on an analog curve peti NATON pa Vr Lhe tet VISIT hesse mie Puyo tam mc Mura Ware ltete no a retro Von POS contri BI Adnan caapi A stu Agra Dott pn Wit ae ti our te rroma bee rap enn d atta Seem Ira rare but cay ead be omoara Da el oe ARTE br o rea m Low maker of athe mod m ner bul doer fose the rezulta Fig 49 Set the pressure and feedback coefficients Set Pressure Digital Readout This example allows you to set a pressure order and monitor the result on a numerical eu ARJ DETLALISATDOM Orennal Mater heere x Sera unter Serial p nn 19 Code precmse Maasse Tone ma messa CS Statua a i top MPC tba Q MECS a reset MCS reeds to be manualy seared green otet on De front panel I Sure Da Dreumes pg Overpreesre J METS needs fo de marini reamed after conpresaze green petre on the boort parel Fig 50 Set the pressure and watch the numeric output
34. tion Pout Pin Pout Atmosphere Fig 51 Pressure must be properly equalized In order to operate in an optimized range of the pressure full scale it is recommended to work with differential pressure Indeed by pressurizing both input and output of a channel properly the pressure inside the channel would be equal to the differential of the output and the input pressures 30 WORLD PRECISION INSTRUMENTS MicroFluidics Control System MAINTENANCE The MFCS is designed as a very robust device for usage in laboratory and industrial environment so it is easy to maintain Calibrate it regularly typically every month or after the MFCS has been displaced in order to maintain optimal accuracy of the results and compensate for offset drift of the pressure sensors The housing can be cleaned with a soft tissue moistened with iso propanol or window cleaning products Avoid aggressive organic solvents as acetone or chloroform or abrasive cleaning products WORLD PRECISION INSTRUMENTS 31 MicroFluidics Control System ACCESSORIES Designation Reference and Supplier USB Cable CD Software User manual Blue tubing 0D 1 32 ID 250um Upchurch Scientific 1581 FEP tubing 0D 1 16 ID 800um Fisher A28556 Glass capillary OD 360um ID 100um Polymicro technologies TPS100375 Green Sleeves 1 16 to 360um Upchurch Scientific F 242X Green Sleeves 1 16 to 1 32 Upchurch Scientific F 247X 10 32 Peek Fitting N
35. uiwell Reservoirs Fig 4 Optional accessories for the MFCS system include a Fluiwell and a set of spare parts WORLD PRECISION INSTRUMENTS 5 MicroFluidics Control System Unpacking Upon receipt of this instrument make a thorough inspection of the contents and check for possible damage Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Concealed damage should be reported at once to the carrier and an inspection requested Please read the section entitled Claims and Returns on page 40 of this manual Please contact WPI Customer Service if any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department Goods returned unauthorized by collect freight may be refused If a return shipment is necessary use the original container if possible If the original container is not available use a suitable substitute that is rigid and of adequate size Wrap the instrument in paper or plastic surrounded with at least 100mm four inches of shock absorbing material For further details please read the section entitled Claims and Returns on page 40 of this manual 4 WORLD PRECISION INSTRUMENTS MicroFluidics Control System INSTRUMENT DESCRIPTION Pressure Inlet USB Power Supply Rear View On Off Switch LCD Pannel Displays i
36. uts Upchurch Scientific F 120 soft tubing 1x3mm Fisher A31309 Micrew reservoir 1 5 mL Fisher W14432 32 WORLD PRECISION INSTRUMENTS MicroFluidics Control System SPECIFICATIONS MECS 25 mBar Characteristics Wi Typ Max unit Comment ____ input pressure range 500 ooo mbar Output pressure precision lt 2 5 full scale Better if calibrated Quick start in output pressure step 23 mar _ ae operation temperature range 5 25 o ec SSS input voltage range 23 a as pe wegnt 19 jz ke Dependsonmoder Power consumption ae fw MFCS 70 mBar Characteristies Min 1yp Max Unit comment ____ input pressure range soo 600 mbar Output pressure precision lt 2 5 full scale Better if calibrated Quick start Min output pressure step 63 fubar Geo an me one eO operation temperature range 5__ 25_ ao e input voltage range Jas za fas LI Weight 1 9 225 kg Depends on model Power consumpton ere n S WORLD PRECISION INSTRUMENTS 33 MicroFluidics Control System MFCS 350 mBar Characteristics Input pressure range mbar Min output pressure step Max output pressure en pa operation temperature range 5 25 o e EEE EEE a Weight 1 9 2 25 Depends on model EINE MFCS 1000 mBar Characteristics Wim iyp Max unit comment _ input pressure range ia ia bar _ Output pressure precision lt 2 5 full B
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