User Manual - Leica Microsystems
Contents
1. Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and products could be contaminated by abraded particles In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms Leica S Series The cleaning of glass surfaces and objec tives in particular should be carried out exclusively as outlined in the brochure Cleaning of Microscope Optics The infor mation can be downloaded at http www leica microsystems com prod ucts Select your product and go to the Down load page For additional information refer to page 51 User Manual Servicing gt Repairs may only be carried out by Leica Microsystems trained service technicians Only original Leica Microsystems spare parts may be used Responsibilities of person in charge of instrument gt Ensure that the Leica stereomicroscope is operated maintained and repaired by authorized and trained personnel only Important Safety Notes User Manual This User Manual describes the special func tions of the Leica StereoZoom stereomicro scopes S series and contains important instructions for their operational safety main tenance and accessories You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the User Manual and the2. Seica MICROSYSTEMS Living up to Life User Manual Leica S8 APO B a CE p Copyright All rights to this documentation are held by Leica Microsystems CMS GmbH Reproduction of text or illustrations in whole or in part by print photocopy microfilm or other method including electronic systems is not allowed without express written permission from Leica Microsystems CMS GmbH The instructions contained in the following documentation reflect state of the art tech nology We have compiled the texts and illus trations as accurately as possible Still we are always grateful for comments and suggestions regarding potential mistakes within this docu mentation The information included in this manual may be changed without prior notice Leica S Series Revision 1 0 published March 14 2013 by Leica Microsystems Wetzlar GmbH Ernst Leitz StraBe 17 37 D 35578 Wetzlar Germany http www leica microsystems com Responsible for contents Marketing CMS User Manual 2 Function of the Microscopes The Leica S8 APO B microscope for which this User Manual has been written is used for routine examinations of cell and tissue cultures liquids and sediments This includes examining specimens taken from the human body for the purpose of gaining information about physi ological or pathological conditions or inborn anomalies or testing for safety and compat ibility for potential recipients or f
3. micrometers for calibrating may be ordered Graticule 10 mm 0 1 mm Graticule 5 mm 0 1 mm Graticule 5 mm 0 05 mm Graticule 100 div 0 002 Graticule 100 div 0 001 Graticule 150 div 0 0005 Crosshair Stage micrometer 50 mm 0 1 0 01 mm graduation Stage micrometer 1 0 001 graduation vY YV VV VY V Vv User Manual Inserting the Graticules The graticules can be inserted into the adjustable eyepieces and into the eyepieces for eyeglass wearers Leica S Series Fitting the graticule s 1 Using the stereomicroscope determine on which side the scale is vacuum metallized The scale must not appear reversed 2 Remove the insert from the bottom of the eyepiece and place it on the bench with the knurled side down User Manual Hold the graticule by the edges to avoid leaving fingerprints and push it into the holder from the side Replace the insert in the eyepiece and press it firmly into place Insert the eyepiece in the tube and turn the eyepiece in the tube to align the graticule correctly Eyepieces You can combine your StereoZoom with Li a fixed or an adjustable eyepiece For equipment with a graticule for photography in one eyepiece two adjustable eyepieces are needed We recommend that you also equip the high powered StereoZoom Leica S8 APO B with two adjustable eyepieces Leica S Series Inserting the eyepieces 1 Push the eyepieces as far as they will go in
4. 25 Tips for Working Ergonomically Align your stereomicroscope opti mally You must configure the settings described here precisely in order to be able to take full advantage of its outstanding optical and ergonomic advantages gt Align your workstation optimally Consider the height of the bench and chair Use the whole seat surface and the back rest gt Ensure that your lower arms are supported gt When carrying out other tasks perform exercises to relax and relieve muscle tension Leica S Series User Manual 26 Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer Simply push them into the tube and they are ready to use What does parfocal mean Parfocal means that a specimen contin H ues to remain exactly in focus even if the magnification on the stereomicroscope is modified All stereomicroscopes from Leica Microsystems are parfocally matched However the parfocality requires a personal dioptric correction for the user Leica S Series Dioptric correction In order to parfocally match the stereomi croscope at least one eyepiece with dioptric correction is necessary The setup is described on the following pages With two adjustable eyepieces from page 35 If you do not wear glasses Depending on the preferences of the observer eyecups can be used A To avoid eye infections we recommend that every user uses his or her o
5. al protection EC Declaration of Conformity gt Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity See page 50 Leica S Series Health risks Workplaces with stereomicroscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the dura tion of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken Optimal arrangement of workplace work assignments and work flow changing tasks frequently Thorough training of the person nel giving consideration to ergonomic and organizational aspects The ergonomic design and construction of the Leica M stereomicroscopy series are intended to reduce the exertion of the user to a minimum User Manual Direct contact with eyepieces can be a potential transmission path for bacterial and viral infec tions of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Safety Instructions continued Light sources safety regulations gt Light sources pose a potential irradia tion risk glare UV radiation IR radiation Therefore lamps have to be operated in closed housings and in installed condition Never look directly into the beam path blind
6. art of the objective Zoom 8 1 apochromatic Viewing angle 38 ESD protection Antistatic Specific surface resistivity 2x10 0 square discharge time lt 2 seconds from 1000 V to 100 V Magnification basic configuration 10x 80x Maximum resolution 600 Ip mm Maximum numerical aperture 0 2 Working distance basic config 75 mm Object field diameter 23 mm Adjustable zoom limits 2 Video photo outlet switchable 100 visual or 100 video photo and 100 visual in the left eyepiece Image acquisition coax lighting Yes Standard objectives lead free Apochromats 0 63x 1 6x 2 0x Achromat 0 32x Ergonomic eyepieces fixed and adjustable with cups 10x 23 16x 15 25x 9 5 40x 6 Interpupillary distance 55 75 mm Leica S Series User Manual 45 Leica TL ST Transmitted Light Base Light source Halogen lamp 12V 20W Quick illuminant change Yes Illuminated area 50mm Power supply Input voltage 100 240 V frequency 50 60 Hz Energy consumption 30 W max Ambient temperature 10 40 C Connections Power plug Weight 7 4kg Illumination modes Bright field Yes Dark field Yes single sided Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control No Integrated filter holder Yes Coated optics for incre
7. asing the color Yes temperature Matching of high num aperture No Remote control options No AntiShock Pads Yes Dimensions WxHxD 340x430x85 mm Leica S Series User Manual 46 Appendix Leica S Series User Manual 47 Calculating the Total Magnification Field of View Diameter Parameters Example Mo Magnification of the additional objective Mo Additional objective 1 6x ME Magnification of eyepiece ME 20x 12 eyepiece Z Magnification changer position Z Zoom position 4 0 Nfoy Field number of the eyepiece Field numbers are printed on the eyepieces 10x 23 16x 16 20x 12 10x 23B 16x14B 25x 9 5B 40x6B Magnification in the binocular tube Calculation example field of view diameter in the specimen MTOT vis Mox ME xz or 1 6x20x4 128 Leica S Series User Manual Troubleshooting The field of view is shadowed gt Adjusting the correct Distance page 28 Interpupillary The image goes out of focus gt Inserting the eyepieces correctly page 22 gt Perform diopter correction exactly accord ing to the instructions from page 35 The focusing drive gradually sinks on its own or is difficult to turn gt Regulate the ease of movement page 33 Leica S Series In the case of failures of electrically oper ated devices always first check Is the voltage selector set correctly gt Is the main power switch switched on Is the pow
8. cality 1 Selectthe highest magnification level 2 Observe the specimen if necessary refocus it slightly 3 Change from the highest to the lowest magnification The sharpness should be constant parfocal If this is not the case repeat this procedure Leica S Series User Manual 37 Leica TL ST Transmitted Light Base Controls Refer also to the separately provided User Manual for the TL ST transmitted light base Extension plate of the transmitted light base TL ST Rear side ofthe transmitted light base TL ST 1 Screws for changing the halogen lamp 2 Power connection socket 3 Main power switch 1 Adapter plate for easy assembly of focusing drives 2 Removable glass plate Controller for light intensity 4 Adjustment for path folding mirror Uu Leica S Series User Manual Leica TL ST Transmitted Light Base Operation Light intensity control The left control adjusts the intensity of the 12 V 20 W halogen illumination 1 Switch on the illumination of the base at the power switch 3 Focuson the specimen 3 Set the illumination to the desired intensity using the left control Leica S Series Transmitted light control The transmitted light base TL ST has a slider that automatically moves the path folding mirror in the base when moved The mirror is kept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light User Manual B
9. caustic and corrosive materials and keep chemicals away from the instruments Plugs optical systems and mechanical parts must not be disassembled or replaced unless doing so is specifically permitted and described in this manual Protect your stereomicroscope from oil and grease Do not grease guide surfaces or mechani cal parts Care Maintenance Contact Persons continued Protection from dirt Dust and dirt will affect the quality of your results gt Put a dust cover over the stereomicroscope when it will not be used for a long time Use dust caps to protect tube openings tubes without eyepieces and eyepieces Keep accessories in a dust free place when not in use Leica S Series Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuit able cleaning agents and techniques can damage polymers User Manual Permitted measures b Clean the stereomicroscope or parts of it using warm soapy water then wipe using distilled water For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow the corresponding safety regulations Remove dust with a pneumatic rubber bulb anda soft brush Clean eyepieces and objectives with special optics cleaning cloths and pure alcohol Copyright Leica Microsystems CMS GmbH Ernst Leitz StraBe 35578 Wetzla
10. e the two metal clamps This prevents the cables from getting caught during insertion Photography with the Leica S8 APO B H The observation beam path and the photobeam path can be switched For this the light splitting is influenced as follows gt Vis position 100 light in both eyepieces but no light in the video photo beam path Doc position 100 light in the right eyepiece but no light in the left eyepiece 100 of the light crosses the video photo beam path Leica S Series The focusing and the framing are done with the left eyepiece video photo beam path Flat specimens are sometimes blurry on H the left and right edge of the image This blurring is based on laws of optics and does not mean there is a malfunction of the camera or the microscope User Manual Capturing images and videos 1 If the image detail and image sharpness are set to your satisfaction switch on the Doc setting and capture your image The images captured with the camera may not be used for diagnosis In this case an evaluation with the eyepieces is required Dimensional Drawings in mm Leica S8 APO B with Transmitted Light Illumination Leica S Series User Manual 43 Technical Data Technical Data StereoZoom Leica S8 APO Optical system 12 Greenough lead free using best corrected central p
11. er cable connected correctly gt Are all connecting cables attached correctly gt Are the fuses intact User Manual Photos are blurry Focus accurately page 29 Bring the graticule into sharp focus and perform diopter correction exactly accord ing to the instructions page 35 gt Insert the eyepieces correctly up to the stop page 22 Check that the graticules are securely in place in the eyepiece page 21 The image from the camera stays black gt Switch the beam splitter on the photo tube to the Doc setting page 41 49 Care Maintenance Contact Persons We hope you enjoy using your stereomicro scope Leica devices are renowned for their robustness and long service life Observing the following care and cleaning tips will ensure that even after years and decades your Leica stereo microscope will continue to work as well as it did on the very first day Warranty benefits The guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Leica S Series EC Declaration of Conformity To download the EC Declaration of Conformity use this link http www leica microsystems com products stereo microscopes macroscopes research manual Select the microscope type and go to the Download page User Manual Care gt Protect your stereomicroscope from mois ture fumes and acids and from alkaline
12. er the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims as well as product liability and the Declaration of Conformity User Manual Place of use gt Only use the instruments in enclosed dust free rooms and between 10 C and 40 C Protect the devices from oil chemi cals and extreme humidity If using the devices outdoors protect them from dust and moisture Never use electrical devices outdoors Electrical components must be placed at least 10 cm away from the wall and away from flammable substances Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort micrographic images for example In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Safety Instructions continued Responsibilities of person in charge of instrument These Safety Instructions must be available at the workplace Ensure that The M series stereomicroscopes and accessories are operated maintained and repaired by authorized and trained person nel only All operators have read understood and observe this User Manual and particularly the safety regulations Repairs service work Repairs may only be carried out by Leica Micr
13. evel H At the lowest magnification the desired specimen detail can be easier to localize due to the large field of view User Manual 3 Look into the eyepieces and insert the desired specimen detail in the center 4 Focus on the specimen with the drive knob Changing Magnification Zooming All stereomicroscopes of the S series Li allow a continuous magnification change The magnification changer can be operated with the left and the right hand The image scale is shown on the right drive knob The basis for the calculation of the total magnification and the field of view can be found on page 48 Leica S Series Changing magnification 1 Look into the eyepieces 2 Focus on the object see page 29 3 Rotate the magnification changer until the desired magnification is configured User Manual Limiting Zoom Range The zoom range can be limited above and Defining the lower limit 3 Set the stop on the left drive knob forward below In the same manner a fixed magnifi 1 Loosen the Allen screws on the left drive until it touches the built in zoom stop cation level can be set The following exam knob with the Allen key provided ple shows the limit to the range between 1 to 3 2 2 Turn the right drive knob to position 1 4 Carefully tighten the Allen screw Continued on next page Leica S Series User Manual 31 Limiting Zoom Range continued Defining the upper limit stop 3 Set t
14. ew from the complete image down to the finest enlarged detail Leica S Series Though the reliability and robustness of Leica stereomicroscopes is world renowned like any high tech product the Leica S series requires a certain degree of care and attention Therefore we recommend that you read this manual It contains all the information you need regarding operation safety and maintenance Simply observing a few guidelines will ensure that even after years of intensive use your stereomicroscope will continue to work as smoothly and reliably as on the very first day We wish you the best of success in your work after all you are now equipped with the best tool User Manual 14 What Your Stereomicroscope Offers You The optical system of the Leica StereoZoom line consists of two beam paths converging at 12 The objective pairs of each optical path are positioned close together so the stereomicro scopes can be of very slender design espe cially towards the base of the instrument The advantages of this design are that it has a small space requirement for use on bonders and in machine applications unobstructed access to specimens plenty of space for tools and a completely clear view of the object field The Greenough system enables cost effective correction of aberrations such as chromasia image field curvature and distortion with mini mal effort In the new Leica StereoZoom line the optimum corrected cente
15. he stop backward on the right drive 1 Loosen the Allen screws on the right drive knob until it touches the built in zoom knob with the Allen key provided stop 4 Carefully tighten the Allen screw Leica S Series User Manual 32 Regulating the resistance of the focusing drive Adjusting the resistance Is the focusing drive too loose or too tight Does the equipment tend to slide downwards The resistance can be adjusted individually depending on the equipment weight and personal preferences as follows 1 Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focus ing Leica S Series User Manual 33 Changing the position of the optics carrier The optics carrier can be turned side Changing position ways in the microscope carrier if the user 1 Unscrew the clamping screw wants to work from the side 2 Turn the optics carrier laterally to the desired position 3 Carefully tighten the clamping screw Leica S Series User Manual Dioptric Correction with Two Adjustable Eyepieces If you set the diopters on the adjustable Li eyepiece exactly as described the image will remain equally sharp and constant parfo cal from the lowest to the highest magnifica tion This means you do not have to refocus when changing magnification The focus needs to be readjusted only if you want to view a specimen detail that is located higher
16. ing hazard Do not select a white strongly reflective background for the specimen Leica S Series Careful handling gt Exercise particular care when setting up the instruments If it is specified that two or more people are required for setup compli ance with this is mandatory Never spill any liquids on electrical instru ments This could cause the stereomicro scope and other equipment to become electrically live and damage people and instruments Never clean instruments using corro sive cleaning agents or those containing acetone For detailed information about care refer to the User Manual for the instru ment Check the power cables regularly Defective power cables can cause injuries Wait for bulbs to cool off before changing them Touching hot bulbs can cause burns User Manual Contents Copyright 2 Function of the Microscopes 3 General Notes 4 Important Safety Notes 5 Symbols Used 6 Safety Instructions 7 Contents 11 Congratulations 14 What Your Stereomicroscope Offers You 15 On We Go 16 TL ST Transmitted Light Base 18 Optics Carrier and Additional Objective 19 Available Graticules 20 Inserting the Graticules 21 Eyepieces 22 Mounting a Camera 23 Leica S Series Quick Start Guide Overview of the Leica S8 APO B 25 Tips for Working Ergonomically 26 Using the Eyepieces 27 The Correct Interpupilla
17. or lower Use this advantage as often as possible it is not available on all stereomicroscopes H The diopters can be set between 5 and 5 Leica S Series The following adjustments have to be H carried out only once by each user Using graticules leads to slightly deviated settings which are described in the user manuals for the graticules User Manual Adjusting the diopter settings 1 Turn the rotary knob to the Vis position 2 Turn the dioptric correction on both eyepieces to the center position Continued on next page Dioptric correction with two adjustable eyepieces continued 3 Placea flat specimen under the objective 6 Setthe highest magnification level 9 Turn the eyelens of the eyepiece as far as it will go in the direction without looking 4 Setthe lowest magnification level 7 Optimize the focusing with the focusing into the eyepieces while doing so drive 10 Look through the eyepieces and close one eye 11 With the other eye monitor the specimen and turn the eyelens of the eyepiece slowly in the direction until this eye sees the specimen sharply 5 Observe the specimen through the eyepieces and bring it into sharp focus with 8 Setthe lowest magnification level 12 Repeat steps 10 and 11 with the other eye the focusing drive Continued on next page Leica S Series User Manual 36 Dioptric correction with two adjustable eyepieces continued Checking parfo
18. or monitoring therapeutic measures IVD The above named microscopes comply with the Council Directive 98 79 EC concerning in vitro diagnostics Leica S Series The manufacturer assumes no liabil A ity for damage caused by or any risks arising from using the microscope for other purposes than those for which they are intended or not using them within the speci fications of Leica Microsystems CMS GmbH In such cases the Declaration of Conformity shall be invalid Leica S8 APO B identification label Leica 1 2012 model ssapo pl ULIUNHIIIIH IN KAIN TMI 25466 11524102 BZ 00 100 240V 50 60Hz max 30W A RK voice Leica Microsystems CMS GmbH Ernst Leitz Str 17 37 D 35578 Wetzlar Q Lamp 12V 20W NS da tr Singapore User Manual These IVD instruments are not A intended for use in the patient environ ment defined by DIN VDE 0100 710 Nor are they designed to be combined with medical instruments in accordance with EN 60601 1 If a microscopeis electrically connected to a medical instrument in accordance with EN 60601 1 the requirements listed in EN 60601 1 1 shall apply Not suitable for examining potentially infec tious specimens This type of instrument may be operated by trained laboratory personnel only General Notes Use in clean rooms The Leica S series can be used in clean rooms without any problems Cleaning gt Do notuse any unsuitable cleaning agents chemicals or techniques for cleaning
19. osystems trained service technicians Only original Leica Microsystems spare parts may be used Leica S Series Before opening the instruments switch off the power and unplug the power cable Avoid contact with powered electrical circuits which can lead to injury arans por Use the original packaging for shipping or transporting the individual modules of the Leica M stereomicroscopy series and the accessory components In order to prevent damage from vibrations disassemble all moving parts that accord ing to the user manual can be assembled and disassembled by the customer and pack them separately User Manual MiegraLone in third party products When installing Leica products into third party products the manufacturer of the complete system or its dealer is responsible for following all applicable safety instruc tions laws and guidelines Apasa Once the product has reached the end ofits service life please contact Leica Service or Sales about disposal Please observe and comply with the national and federal laws and regulations that are equivalent to EC directives such as WEEE Like all electronic devices the micro scope its accessory components and consumables must never be disposed of with general household waste Safety Instructions continued Legal regulations gt Observe the generally applicable statu tory and country specific regulations for accident prevention and environment
20. r Germany 2013 Phone 49 6441 29 0 Fax 49 6441 29 2599 LEICA and the Leica logos are registered trademarks of Leica IR GmbH Printed on chlorine free bleached paper III 13 M H Revision 1 0 issued March 14th 2013 CE www leica microsystems com eca MICROSYSTEMS
21. r of the objec tive is used for the image This provides supe rior optical performance with large level and undistorted fields of view and chromatically optimized high contrast images Leica S Series Photography The StereoZoom Leica S8 APO B model is equipped with an integrated video phototube allowing for easy quick digital camera assem bly Leica MC170 HD User Manual Apochromatic correction The Leica S8 APO B is a completely apochro matically corrected Greenough system The apochromatic optics correct chromatic aberra tion perfectly remove bothersome color seams and display pin sharp images of even the finest enlarged detail The contrast brilliance sharp ness resolution color fidelity and image preci sion are unsurpassed The benefit of apochro matic correction is best seen in specimens that have a fine low contrast structure such as large animal cells cilia plants or metallic microelec tronic structures The technical features are found on page 44 On We Go If your new Leica stereomicroscope has already If on the other hand you are assembling the been assembled and commissioned by your stereomicroscope yourself continue with the Leica consultant click here to skip through the Assembly chapter which begins on page installation instructions and go directly to the 17 Quick Start Guide on page 24 Leica S Series User Manual 16 Assembly Leica S Series User Manual 17 TL ST T
22. ransmitted Light Base Unpacking the base The base is delivered with the adapter plate installed Make sure the instruments are unpacked on a flat sufficiently dimensioned and non slip surface Refer also to the separately provided User Manual for the TL ST transmitted light base Leica S Series Focusing drive and column unscrew the 3 screws A Never on the right side of the focusing column 1 Unscrew the extension plate from the base using the Allen key provided 2 Attach your focusing drive column to the bottom using the 4 Allen screws User Manual Reattach the adapter plate to its original position using the 6 Allen screws Optics Carrier and Additional Objective Optics carrier Additional objective optional Protective objective glass optional 1 Insert the optics carrier carefully in the 1 Screw the desired objective counterclock 1 Screwtheobjective protective glass directly microscope carrier and fasten it in the wise into the optics carrier onto the StereoZoom or on the additional desired position with the clamping screw objective Leica S Series User Manual 19 Available Graticules The optional graticules enable measure ment and in addition provide valuable information when comparing and capturing still images of the specimens Insert the grati cule before you set the eyepiece in place Leica S Series Available graticules The following graticules and stage
23. res 1 Numbers in parentheses within the descriptions relate to the figures and the items within those figures User Manual Disposal Notes on how to dispose of the micro scope its components and consum China RoHS 50 year EFUP Environ mentally friendly use period IVD labeling nstrument for in vitro diagnostics vo IVD manufacturing date for example 11 2011 for MM YYY November 2011 Safety Instructions Description The individual modules fulfill the highest requirements for observation and docu mentation of Leica stereomicroscopes of the M series Intended use of instrument Leica Microsystems microscopes are opti cal instruments for improving the visibility of objects or specimens through magnifi cation Accessories such as optical acces sories stands illumination cameras etc supplement the equipment configuration Non intended use Using the instrument in any way contrary to the specifications in the User Manual can lead to bodily harm and damage to objects Never use microscopes for in vivo examina tions or eye surgery if they are not expressly intended for such use Never install any other plug or unscrew optical systems Leica S Series and mechanical parts unless expressly instructed to do so in the instructions The instruments and accessories described in this User Manual have been tested for safety and potential hazards The responsible Leica affiliate must be consulted whenev
24. right field Bright field is suitable for examining translu cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background Move the slider backwards until the desired effect is achieved Inclined transmitted light Transmitted light that traverses the object obliquely will provide additional resolution and information when observing semitransparent opaque objects gt Slowly pull the slider towards yourself until the desired effect is achieved Leica TL ST Transmitted Light Base Lamp Replacement Changing the halogen lamp Before you change the lamp it is abso lutely necessary to unplug the power plug from the base to prevent the risk of elec tric shock The halogen lamp becomes very hot during operation Therefore to avoid being burned let the base cool off for approx 10 minutes after switching it off A Do not touch new halogen lamps with your bare fingers this drastically reduces the service life of the lamp Leica S Series Changing lamps 1 Unscrew the two screws on the cooling unit and pull the cooling unit out along with the lamp 2 Carefully pull out the lamp and mount by pulling them upwards 3 Disconnect the lamp from the mount 4 Insert the new lamp into the mount and reinsert the lamp holder User Manual Safety precautions A When inserting the lamp ensure that the cables are insid
25. ry Distance 28 Focusing 29 Changing Magnification Zooming 30 Limiting Zoom Range 31 Limiting Zoom Range continued 32 Regulating the resistance of the focusing drive 33 Changing the position of the optics carrier 34 Dioptric Correction with Two Adjustable Eyepieces 35 Leica TL ST Transmitted Light Base Controls 38 Leica TL ST Transmitted Light Base Operation 39 Leica TL ST Transmitted Light Base Lamp Replacement 40 Photography with the Leica S8 APO B 41 Leica S8 APO B with Transmitted Light Illumination 43 User Manual 11 Technical Data Technical Data 45 Leica TL ST Transmitted Light Base 46 Calculating the Total Magnification Field of View Diameter 48 Troubleshooting 49 Care Maintenance Contact Persons 50 Leica S Series User Manual 12 Leica S8 APO B Congratulations Congratulations on obtaining your new Leica StereoZoom line stereo microscope S series We are convinced that it will more than meet your expectations This instrument embodies all the qualities you associate with the name Leica Microsystems excellent objectives high quality engineering and reliability Furthermore the modular design ensures that the Leica stereomicroscope adapts perfectly to your needs no matter which accessories you require for your tasks Thanks to the parfocal system you can always obtain a precise sharp display of your microscopic specimens at both large working distances and large fields of vi
26. safety instructions from the supplier Leica S Series Before installing operating or using the instru ments read the user manuals listed above In particular please follow all safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals User Manual We guarantee the quality of our products Our guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Symbols Used Warning Safety hazard This symbol indicates especially impor A tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel gt Functional disturbances or damaged instruments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged instruments Leica S Series Danger due to hot surface A This symbol warns against touching hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that are intended to provide clarity Explanatory notes gt This symbol within the text stands for addi tional information and explanations Figu
27. to the tubes 2 Check that the eyepieces are seated firmly and precisely in place User Manual Risk of infection Direct contact with eyepieces is a poten tial transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Mounting a Camera The Leica S8 APO B is equipped with H an integrated video phototube which allows the simple fast mounting of digital cameras for photos and video Ask your Leica sales representative for more details Leica S Series Installing the camera 3 Insert the unit into the video photo output 1 Remove the protective dust cover from the of the stereomicroscope and screw it in video projection lens C mount adapter and the microscope camera 2 Screw the camera into the video projection Always close the video photo output lens C mount with the protective dust cover if no camera is installed User Manual 23 Quick Start Guide Overview of the Leica S8 APO B 1 Eyepieces 2 Adjustable tubes Interpupillary distance adjustable from 55 75 mm 3 Magnification changer right drive knob with magnification scale 4 Fixing screw for optics carrier in the microscope carrier TL3000 ST transmitted light base Focusing drive Stop for zoom limit C mount adapter and video photo tube Leica MC170 HD camera kit optional 0 AN SS NHN Leica S Series User Manual
28. wn pair of eyecups User Manual If you wear glasses Eyeglass wearers must remove or fold back the eyecups Fig below left as otherwise they cannot see the entire field of view The Correct Interpupillary Distance H The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen If you are still a novice microscope user you may need a short time to become accustomed to this Not to worry after a little while it will become automatic Reference values The interpupillary distance can be set between 55 and 75 mm An exit pupil distance is the distance between eye and eyepiece With the 10x 23B wide field eyepiece for eyeglass wear ers it is approx 22 mm For those who do not use the eyepiece for eyeglass wearers it is 12 mm Leica S Series Adjusting the interpupillary distance 1 Bring the eyes slowly to the eyepieces Push the tubes together or apart with both hands until you see a single round circular image field without shadows with both eyes User Manual v Focusing Focusing raises and lowers the stereomi Li croscope using the focusing drive The specimen detail is brought into sharp focus as soon as it isin the focal point of the objective H The focusing drive can be operated either left or right handed Leica S Series Focusing 1 Align the specimen under the objective 2 Set the lowest magnification l
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