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Protein Solutions DynaPro-99 Software Manual

1. 2791 9 36 Aves Mona n 7n amp 125 16 22 4 3 0 08 2492 1 100 19 2 894333 1 001 6 594 Bi 1 0202 286 8 55 z7 n 3 4 183 2 894333 1 001 2123 Bi 2 O509 107 18 5 4183 7 26 b ark All Mono All Bi i E Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Show All Bimodal Analysis Help Coefficients ulel o30 Show All Bimodal presents the Bimodal size MEL MEN oo bimodal Result distribution fit for all measurements within the Regularization k Mark as Excluded Dynals NEM Show AI M onamadal experiment Static hw Model Show All Bimodal Calculators EEE e For example quickly view the bimodal scatter SOES Hist T i P i Recalculate All Ta diagram to confirm 1f the size distribution contains T m Scatter Diagram two eaks Regression Fit Error p Scatter Diagram bsa unfiltered exp Meast Rad nm MW kDa XM Meastt Rad nm MW kDa Mass eas ad nm a Mass 3 73 66 1184327 100 0 fa 365 08 4757924 100 0 124371 93079392 o0 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Histogram View arepresentation of the size distribution RR GR histogram for the active measurement and Analusis Oe Coefficients En l A selected result monomodal or bimodal Curnulants Show Bimodal Result
2. Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Count Rate Mode New Experiment Dynamics File Tools Analysis Help E E E A A Count Hate Measurement Count 356 top Fh nm la n0 E Start t Temp 23 6 23 6 e Dal 0 0 Joo sv Exit Count Rate Mode by pressing the stop button S E N E T 1 Optionally monitor Temperature versus time by clicking the radio button Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Measurement Mode New Experiment Dynamics File Tools Analysis Help o Deal IL Af ees Lhe da oo o Count Aate mm Measurement m g Start Ig f Count 402 378 J Rhim 200 p 00 C Temp jae 236 MwIKDa Lr foo e E o Enter the Measurement Mode by pressing the Start Button 1n the Measurement Mode Window READ Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Press the start button and take data Collect 10 20 data points QuickStart Measurement Mode Radius MW information t ESA ESR 2HG HL 02 FILTEH exp Dynamics Fie Tools Analysis Help Osla C Wel ale a we N icid Data should Im arrive on the Count Rate Measurement nos screen every D Deane EUN NE jo mem RS Rr 7 few seconds
3. Show or hide the Tool Bar Options Options Graphs Timeouts Toolbars Baseline Tal Cirectorie lal Experiment Dynamics File Tools Analysis Help ee 1 a mccum p mm Tx Awg E Rupee iL IE O Count o P Fih nm po pn EN I Show Status Bar Temp foo o o an MwikDa 0 0 fo amp Cancel ee 4 Show or hide the Status Bar nd Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Scheduler New Experiment Dynamics File Tools Analysis Help k 3e ES u joo D Options MS an e pns Measurement Tae Scheduler zi INI Fh nm 0 00 0 00 3m Connect E Mire Hosh foo Ue Iw ED a oo foo sv i Scheduler Use the Scheduler to perform time delayed measurements Measurement schedules are Close Aun Status Ready Current Exp Time Elapsed 00 00 00 Time Ta wai 00 00 00 Curent Meas U Curent Temp 0 0 Stop saved as text files with extension dss Dyanmics Schedule Script Filename Append Mew Schedule automated measurements M ewE periment Set emperature T akeMeasurements MewE xperiment SelTemperature T akeMeasurements O01 Ha exp m T 15 Delete Save Save As new 2 exp 20 6 15 options Right click For more editing Artisan Technology Group Quality Instrumentation Guara
4. bsa unhiltered_exp Dynamics e Annotate the experiment e Save the data for future use and study File Tools Analysis Help Select Tools Settings Annotations and Edit Settings to record important experiment information Select File Save to archive the data set ew 2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Printing Data bsa unfiltered exp Dynamics 8 e Select File Print File Tools Analysis Help sg tj aele jm a WO ae OO mmy salele 0 Setup to chose the Count Hate jm d Measurement ne gt t Count 481 440 EE Rh nm 228 62 242 33 prin CT C Temp 25 0 25 0 pax 2 i hw D al j S2 8584 f bo be sv e Then select File 300 T 1500 Print to create our 40 semi custom report Hin Annotations Use the check Results Summary Parameter Settings 20 E mdai bada boxes to indicate 1n Regularization Datalog the information you Count Rate amp Measurement Graphs Bimadal Scatter Diagram want to include Regularization Histogram Cumulants Histogram F Curent hess Only then press OK E C AlliMessurenents ee Autocorrelation Caeffs Curent Mess Only C OST Measurements Po cumu Regression Error Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Menu Commands File Tools Analy
5. Experiment Settings x Data Filtering Solvent hw Madel Conditions Annotations Hardware Canfig Hardware Settings Title Enter experiment title here L omments Apr 5 15 28 2000 Dynamics v 5 25 44 Enter comment here Enter as much information and detail about the experiment as you wish File Information Time Stamp Wed 05 Apr 2000 04 28PM File Version B zh 44 Last Modified Wed 05 Apr 2000 04 28PM File Created Weed 05 Apr 2000 04 28PM Cancel Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools oettings Hardware Configuration Experiment Settings ES Data Filtering Solvent Mw Madel Conditions Annotations Hardware Lontig Hardware Settings Host Unit Seral Number 385 E dit OynaPro 99 YL Reduced 105 Correlator Fi 2 8 Flex 99 usb correlator Optics Black Selection MS s n 088 Model Name Senal Mao Wavelen Angle Cy Count Flust MS fl Bete BOODO 12 jag Fr Host Unit Type APO Bias Normal 1005 U Cancel After attaching the modular optical compartment or device to the DynaPro set DYNAMICS accordingly to incorporate the wavelength scattering angle and clean water count rate of the device The host unit serial number type and correlator settings should correspond to the configuration Artisan Technology Group Quality Instrumenta
6. Regularization Mark as Excluded Monomodal Histogram Measit 1 Inrxi Duynals IE 7 Show All Monoemaodal Radius nm Mw kDa Fold nm of M A Show All Bimadal ew 4 48 12 0 IE 33 4 alculators os Histogram Polyd Index 017 Recalculate All Datalog Scatter Diagram Regression Fit Error Static MY Model Also available as an Icon esi LES JE A Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Datalog Analysis Help Coefficients EN dl OTA The Datalog lists the details of each Se Show Bimodal Result measurement within an experiment Regularization Kk Mark as Excluded Dynals WR oc cil ce Show All M onomodal Static Mw Model Show All Bimaodal Calculators eo Histogram Cumulants DataLog 080494 7_exp OP x Recalculate All Datalog Time s amp mpl Diff Fadius nm Pelyd nm Poledinds M wv EDa Mass Temp C Count Rate Baseline Sos Error p 1 0 0 O246 6235 4 48 1 5 0 11 110 0 100 30 9 105059 1 005 0 305 ar 54 O20 606 453 1 2 0 07 112 0 100 30 2 113106 1 013 0 344 Regression Fit Error 3 109 0 0248 6235 4 43 1 1 0 06 106 0 100 30 4 101520 1 002 0 467 4 1370 0238 6241 4 52 1 5 0 12 112 100 31 1 104076 1 006 0 422 5 1640 0 252 635 442 1 6 0 13 106 0 100 31 1 101444 1 001 0 496 E 1820 0 261 641 4 30 1 4 0 10 99 0 100 30 4 38833 1 001 0
7. gt wv Count Rate mU l Measurement me fe Count n o Sh nm pn po Start C Temp no po e Cancel Checking DynaProgs Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Ready Mode The connection is complete when the DynaPro displays Ready and the DYNAMICS status bar indicates Connected The two soft lights will both be red V New Experiment Dynamics Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Data Collection Prepare and place sample into the MicroSampler following the instructions in the DynaPro manual e Then examine the count rates or intensity of the scattered light by pressing the Count Rate Button Check the count rate fluctuations Ideally the fluctuations should be within about 15 of the mean Click the Start button in the Measurement frame to begin recording data acquisitions e Approximately 10 20 measurements will provide adequate data for each experiment After collecting data refer to the data interpretation tips of this manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Count Rate Mode New Experiment Dynamics File Toole Analysis Help E E AE A lelei Count Rate Measurement Count n l E jo p J Rhim foo po Temp
8. A rtisan Artisan Technology Group is your source for quality Pee new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment A EQUIPMENT DEMOS HUNDREDS OF Instral e REMOTE INSPECTION LOOKING FOR MORE INFORMATION TO qii Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com 7 information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED ee Mus Contact us 888 88 SOURCE sales artisantg com www artisantg com PROTEINSOLUTIONS wa DYNAMICS Version 5 L rHAMIDS Software Manual October 2001 750 Vassar Ave Lakewood NJ 08701 U S A Tel 732 367 1663 Fax 732 370 00324 www protein solutions com Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Welcome e Thank you for choosing to own a DynaPro Molecular Sizing Instrument with DYNAMICS software e DYNAMICS is a Windows based software interface providing advanced control over the DynaPro instrument and data c
9. Apply power to the DynaPro New Experiment Dynamics Ele Tools Analysis Help woo Measurement e Bh nm o 00 o 00 Mwikbay oo X oo Count Rate From within on a DYNAMICS select epo FEO Tools gt Connect a Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Connect The status bar located at the bottom of the panel will indicate the DynaPro is being connected to the PC Ele Tools Analys ste a TUE Li EN n IBI IIS mi liie re eme Avg o0 dum RN stat Rh nm pon Exe C Temp 00 po Q MwikDak 00 joo com aE Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Connect The following error messages indicate the connect was unsuccessful Try Connect again or see Troubleshoot Communications Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Connect e The status bar will indicate the status and progress of the connection The progress indicator will count towards 10046 and the two soft lights 1n the lower right hand corner are off during the connect which 1s typically complete 1n about 15 seconds Mew Experiment Dynamics Oy xi Eile Tools Analysis Help Deje kj uu wNfoooo
10. Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings Conditions Annotations Hardware Config Hardware Settings Data Filtering Salvent Mi Model Conditions pH Js Concentration mg ml 11 856 Buffer Detergeri Selection MA Ac CI j Selection none FPES Select tritam i Select Ammonium Sulfate SOS Add Add T Edt Delete T Delete me Save parameters associated with the sample These values do not influence the size and MW from the dynamic light scattering The correct concentration has to be entered for static MW analysis 1f desired Buffer Detergent and pH are recorded but treated like comments in the data analysis Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Options Mew t upanmeni Dynamics aM Scheduler E Connect a Hos 1e DEL xi L Graphs Timeouts Toolbars Baseline Tal Directories ooo View and edit the i Lancel MESES Soe eee m Rhin fooo ooo sw 1 dl d Tabbed Menu for different display and operating options Directories contains the path to DynaLS The other tabs control various display options for the DYNAMICS graphics window Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com
11. C Temp 50 25 0 RA Mwika fes g5 8 ntensity MW and Size Graphical display of the mean information Radius standard deviation from the Gaussian size distribution fitting algorithm are shown 1n a number of ways ee 4 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Measurement Mode BS4 ESA 2MG ML 02 FILTER exp Dynamics File Tools Analysis Help 5 e Press the Stop D eg d E A Els lul EE aln nns 4 b button and Count Hate Measurement Avg Avg E 1 d umani NER Set anton ROS eo automatically C Temp 250 25 0 RA MWwikDa 88 1 85 8 sv calculate iL bimodal and regularization algorithms e Pressing Cancel halts fitting Cancel Calculating Monomodal Results a 603 idw um Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Data Interpretation BSA SU EM ML 02 FILTER exp Dynamics File Tools Analysis Help eee ee Count Rate M er Avg Ease UAE EB f Count E Regu Histogram BSA ESR 2MG H ASIE t Temp 2 e This button activates a display of the Regularization Algorithm result Here it indicates a monomodal size distribution R s nmi qu Scat X Mass istMBan in 239 imo jc Sc 60 2nd Mean E iih 3rd M ear n ed Artisan Technology Group Quality Instr
12. Mark as Excluded Show All Monemadal Histogram Datalog Shaw All Bimadal Scatter Diagram Regression Fit Error When viewing certain windows such as the Datalog or Scatter Diagram it is helpful to quickly view results from the monomodal or bimodal fits Show All Monomodal presents the monomodal results for all measurements within the experiment Cumulants DataLog 32nmstd_exp Oe x1 Time s Ampl Diff Radius nm Polyd nm Poldinds MwiKDal Mass TempiC Count Rate Baseline Sos Error 1 10 0 0 693 125 15 38 5 1 0 14 2553 3 100 19 2 304469 1 001 11 458 2 20 0 0 710 126 16 26 3 0 0 03 2507 8 100 13 2 589793 1 002 7 058 3 31 0 0 710 128 16 07 a7 0 05 2438 4 100 19 2 995332 0 999 3 369 4 41 0 0711 127 16 16 4 5 0 08 2458 8 100 ee 587981 1 001 4 4832 Ayes Cumulants DataLog 32nmstd exp Pihl Mona 0 706 126 1522 4 3 0 08 2482 1 1 l E Ei 1 0 202 206 858 LL LL 700 4 1 Time s amp mpl Duff Radius nm Folyd nm Polydinds M ve EDa Mase TemplE Count Hate Baseline Sos Error 1 10 0 0220 204 10 06 783 2 79 19 2 304469 1 001 arar Bi 2 O509 107 19 67 4183 7 E seca H Pee P 4 2 20 0 0523 148 13 56 1702 7 44 19 2 209793 1 002 2 2685 0 191 B2 24 95 7104 6 E 5 31 0 0024 425 484 132 5 58 19 2 895432 0 999 1 970 Mark All Mano All Bi D 689 124 16 57 2624 6 42 4 41 0 0042 370 555 185 1 B4 13 2 257991 1 001 1 510 0 675 121 17 00
13. Mona 0706 126 16 22 43 0 08 282 1 100 13 2 834333 1 001 6 594 Bi 202 56 858 PD 3 E 13 2 934333 1 001 2123 Bi z 0509 107 19 67 4183 7 zh hd ark All Mano All Bi Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons Cumulants Scatter Diagram Scatter Diagram 37nmstd exp Meastt Hadi nm MY kDa Mass n 555 i851 eas hzo 2913 363 A Press the cumulants Scatter Diagram Icon to view the amplitudes of all measurements All Mono Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons Regularization Histogram Regu Histogram Lys 004 exp OF X Radius nm Fold nm Polyd Intensity Mass All Sup 1st Peak 2 05 0 15 B 100 0 100 0 Sup 2nd Peak Sup 3rd Peak Peaks f Intensity Press the Regularization Histogram Icon to view the results of the Regularization Analysis Optimal Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons Regularization DataLog Regularization DataLog 32nmstd_exp IDE x Radius nm Dufficm s 27 XScatter zxMass 0 00021 10 10 ze L 2 2 a d 0 00023 10 82 1 3e 007 3 8 14 4 0 00025 11 54 1 8e 007 B 3 5 3 0 00025 12 38 1 6e 007 11 5 16 4 0 00031 14 42 1 4e 007 13
14. 0 13 5 0 00034 15 66 1 3e 007 13 2 10 4 0 00037 17 31 1 2e 007 12 5 6 0 0004 18 75 1 1e 007 11 2 Ia 0 0004 20 193 1e 007 4 4 3 6 0 00046 21 63 3 be LlLIao 53 2 0 00045 23 006 o Je 00 5 0 1 3 3 00055 zh db de O06 LI LI LI LI Press the Regularization DataLog Icon to view the detailed results of the Regularization Analysis 1 3e 007 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons DynaL S V jDTMALS 32nmstd tdf File Settings Options Help Data and Fitted Curve Blpistribution Press the DynaLS Icon to automatically open the EE F Dynal size distribution Mag a A E mim of analysis Note DynaLS isa 4 i separate program and must o m mom mom om om om Nu Me ee be installed on your Residuals x100 computer for this icon to na 693 uj Work Position 01659 l Area 0 010591 Ponsition 0 00296 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons Measurement View Use these Measurement Control Arrows to slow or fast scroll forwards or backwards through the data set Fast Fast Reverse Forward Reverse One One Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Algorithms DYNAMICS offers three size distribution fitting algorithms e Monomodal Gaussian Size Distributio
15. E Static Tmelist 0957892 fea e The coefficients cover time delay from 960ns on L7 t oN e The coefficients associated with molecules less than a A approximately 20 25 nm in MI radius decay to 1 000 within 0 9 10 De 01 iMeH0 10e401 LDeHI2 IMeHS 10e404 100e 05 10eH06 Dex milliseconds Time ius The decay rate is related to the diffusion of the molecules Also available as an Icon esi e E JE A Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Analysis Help Coefficients Curnulants Regularization Dynals Static hw Model Calculators Recalculate All Mark as Excluded W ES oan Show Bimodal Result Show All Monamadal Show All Bimodal Histogram Datalog Scatter Diagram Regression Fit Error Analyze the monomodal and bimodal results View the bimodal result in an open window Exclude or include the selected measurement in the average regularization and DynaLS calculations View all monomodal or bimodal results in the datalog or scatter diagram windows View the Histogram View the Datalog View the Scatter Diagram View the Fit Errors Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Show Bimodal Analysis Help Coefficients Li
16. experiment single click a measurement to activate 1t in all windows Double click it to swap between monomodal and bimodal results Print or Export the data using the File Print Menu command Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Datalog Units Cumulants DataLog 080494 7_exp Oe Merit Time s amp mpl Diff Radius nm Palyd nm Poludind MwiKDal Mass Temp C Count Rate Baseline Sos Error m 1 0 0 248 6235 4 48 129 0 11 110 0 100 30 9 105055 1 005 0 305 Msr ordinal of each measurement of the Polydispersity index is the relative amount of experiment polydispersity Cp Rh Time s elapsed time stamped on each e Est MW calculated from Radius using the measurement selected model in kDa Ampl Amplited is the y intercept of the Pct Mass fraction of each component in the autocorrelation function bimodal size distribution Diff Coef actual translational diffusion Temp C temperature of the optics block coefficient at the recorded temperature in 10 holding the sample 2 cm s e Count cts s 1 second average intensity of Rad nm hydrodynamic radius calculated light scattered from sample from Diff Coef from the Stokes Einstein e Base Line the value of the last equation autocorrelation coefficient Polyd nm standard deviation of the l l e SOS Error the sum of
17. foo joo ES US UI Joo lt Enter count rate mode by pressing the Start Button in the Count Rate Window READY Connected to 47E ea 4 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Count Rate Mode New Experiment Dynamics File Toole Analysis Help Oall A LAP Lk a fooyoo eja Count Rate Measurement ius 1 Avg te Count 399 jog 1 E Fih teal pp po tart C Temp 50 0 50 0 3 Mika Joo foo The DynaPro begins counting photons and displays the intensity of scattered light in thousands of photons per second COUNT Connected to 47E zu led Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Count Rate Mode Mew Experiment Dynamics Count Hate Measurement Ele Tools Analysis Help d User Tip Verify Daji tm eje les we ooo the intensity is not too Avg Count 378 404 C Temp 23 6 23 6 Avg FT high Maximum count iih mm pm AM rate of 8 000 kcts s and M E does not fluctuate more than 15 about the average before taking The average intensity 1s data Refer to Sample displayed in addition to the Prep tips if the real time 1 second average intensity intensity 1s fluctuating Always ensure that the count rate is below 8 000 kcts s to prevent damaging the detection system
18. k Me l CI Regularization gt ar Enter Radius nm 1 3 Ose Dynals H Static Mw Model 23 i Calculated Molecular w eight by B Model Calculators EI Min Concentration Axial Ratio Recalculate All l Standard Curve MY kDa fi 37 Apparent Fraction mpm cular Weight Vol Shape Hydration MY kDa E 18 Calculate the MW from the hydrodynamic radius using the standard Curve and Vol Shape Hydration Models The molecular weight model can be selected under Tools settings MW Model Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Recalculate adl e Recalculates all monomodal bimodal and Cumulants gt regularization algorithms Regularization d e Recalculate to start a previously cancelled Static MY Madel EL NJ calculation Recalculate All Cancel Calculating Bimodal Results 50x EE a The status bar indicates the progress towards completion of the calculations Press the Cancel button to end the operation e NOTE The Recalculate All option will cancel all manual data marking and reapply any Data Filter settings Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Help Help e Review the Contents of the on line help Lonterts e Review Instructions and Suggestions for Using Windows software Using
19. the count rates fluctuate more than 15 over the duration of the acquisition time or to monitor real time changes When working with stable systems at or near the sensitivity limits of the DynaPro use a longer acquisition time and or reduce the the S N ratio to 1 5 The suggested setting 1s 5 10 sec Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings Hardware Settings S N Threshold Expenment Settings Data Filtering Solvent Mw Model Conditions Annotations Hardware Contig Hardware Settings Acquisition Time sec sensitivity S M Threshold 25 amp z SPCM Temperature deg C 20 Communications Device Temperature Controller Settings Part COMe Settings 2400 M 8 1 Cancel The suggested minimum S N ratio ratio of intensity of light scattered from the sample to the light scattered from solvent alone should be 2 5 1 For example 1f the clean water count rate of the instrument 1s 4000 counts s the minimum count rate for obtaining a measurement would be calculated to 10000 counts s However when working near the limits of sensitivity the count rate from the sample may be slightly below this limit say 9000 In this instance set the S N ratio to 2 creating a threshold of 8000 counts to obtain data In this instance it 1s suggested that the acquisition time be increased to 10 seconds or more
20. the squares of the error Gaussian model the monomodal fit actual coefficients minus fitted coefficients Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Datalog Buttons Mak AllMono AIlBi e All Mono Press to view the monomodal fit of all of the measurements from the experiment e All Bi Press to view the bimodal fit of all measurements e Mark Press this button to exclude the selected measurement from the datalog average Regularization calculation and DynaLS Save As command Close Closes the Datalog window Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Scatter Diagram Analysis Help Coefficients Lalu E ilin ozy Cumulants Show Bimodal Result Se EL Regularization Mark as Excluded Meas Rad nm MW kDa X Mass oes ene ene LLL LLL LLL SERRE 7 4 43 107 0 100 0 Dwynals TENE Show All Monamadal Static Mv Madel Show All Bimadal Calculators k l ETET EES Histogram Recalculate All Datalog E Scatter Diagram Regression Fit Eror Also available as an Icon Dem rj wes ule mJ The Scatter Diagram is an X Y Plot of the Radius and Amplitude of each unmarked measurement of the experiment View all Bimodal results with the Scatter Diagram to quickly ascertain the modality of the size distributio
21. 0 73 Axial Ratio Pralate 2 36 Measured Hadius nm 3 3 Axial Ratio Oblate 2 42 LE TI y Recalculate All Molecular Weight Determine the approximate shape of your protein when you know the MW specific volume and hydrodynamic radius e The axial ratios for both prolate football and oblate discus ellipsoids are also calculated Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Calculators Apparent Fraction Coefficients al Eg rm 017 mem e Calculate both the number and Regularization d 5 e e Dynal mass fraction of each species in a Static Mw Model Min Concentration WO species system E E S Axial Ratio Recalculate All Apparent Fraction Molecular weight Apparent Fraction Calculator CR XI Close l Enter Data Measured Radius nm 3 Species 1 Hs Radius nm f s Mol weight kDa 14 ER Number Mass Species 1 1 057 568 Species 2 X f 4 3 13 2 Apparent Fractions Species z qp Radius nm 5 o A Mal weight kDa E4 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Calculators molecular Weight Analysis Help Molecular Weight Calculator Coefficients al Eg aln 01 Curnulants
22. 626 T S000 0254 629 443 1 3 0 08 107 0 100 30 8 96200 1 000 0 337 8 23580 0 254 629 4 40 1 0 0 05 105 0 100 30 5 100275 1 002 0 266 3 265 0 O264 640 4 35 1 0 0 05 102 0 100 30 7 97930 1 001 0 551 10 263 0 0 268 627 445 1 6 0 13 107 0 100 30 8 6647 1 000 0 549 11 301 0 0273 631 4 40 1 3 0 09 105 0 100 30 7 qf F53 1 001 0 395 12 3190 0276 E43 432 0 7 0 02 100 0 100 30 6 96646 1 000 0 342 Aves Mono 0 259 631 4 41 1 3 0 09 105 4 100 30 7 39671 1 002 0 434 Bi 1 0 085 21 2 22 30 6 85 30 99871 1 001 0 269 Biz 180 485 6 69 433 4 15 zi Also available as an Icon Mak AlMono aisi Ege IL ejaj u li Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Datalog Cumulants DataLog 32nmstd exp Time s amp mpl Diff Radius nm Palyd nm Palydinds Mw KDa XMass Temp C Count Rate Baseline Sas Error E 10 0 0693 125 15 38 b 0 14 5h33 100 19 2 404469 1 001 11 458 2 200 OF10 125 16 26 3 0 0 03 ZH 100 13 2 mta Pa Faka G 1 002 T058 3 310 OFT 128 16 07 af 0 05 2438 4 100 19 2 aia ae a 0 999 3 369 4 41 0 OFT 12 16 16 15 0 03 466 0 100 19 2 aarda 1 001 4 492 AVES Mona 0 706 125 15 2 13 8 243 1 100 19 2 034 333 1 001 5 534 Ei 0 202 256 6 56 anos 00 9 E 13 2 034333 1 001 123 Biz 0 509 107 19 67 4183 7 zb Mark All Mono All Bi The Datalog lists all details of each measurement from an
23. E An Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis DynaL S Analysis Help Coefficients Cumulants LL Regulanzation Duynals a a e Automatically opens the ea E at m DynaLs size distribution analysis static Mw Model Calculators k J D 1 MEUSE NENNEN NONO NM EE REEL N m Fa e Note DynaLs is a separate software program and must be installed on the computer for this menu command to function DA BAA RN RAUARNAAUGURARUNAUURR AURA AUOGRNAUEGRRAUGRRAUGURGRRR AUDRR AURA Position 0 002355 Mean 0 0331 STD O 110 3 33 No peaks 2 Peak l Area 0 343 Position 0 00235 E p T Also available as an Icon STD 0 00116 391 Peak E gs LA EB nl Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Calculators Analysis Help e our calculators are available for Coefficients a tn ow quick access to convenient umulants Me Regularization m Regular t gt sed information Static MW Medel RA M e Calculate the minimum concentration Calculators Min Concentration e bob required for a measurement ecalculale EE zm Apparent Fraction Molecular Weight Determine the shape of your protein Find the apparent fraction of species 1 in a two species mixture
24. Help About Dynamics 080494 7_exp Dynamics e View the version number of DYNAMICS installed on the computer Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Help About DYNAMICS Help Contents Using Help e View the version number of DYNAMICS installed on the PC e URL to Protein Solutions website About Dynamics About Dynamics DYNAMICS Im Version 5 25 44 e DynaPro Dynamic Light Scattering Instrument Control Software for Molecular Research Telephone number for customer support http P n pratein solutians com Bu4 81 7 717 Protein Solutions Inc 1994 2000 All Rights Reserved Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons Overview The graphical user interface of DYNAMICS provides ten 10 1cons and four 4 arrows for quick control over files viewing data and analyzing data New Experiment Dynamics File Tools Analysis Help olsi t reps ee aj OOOO l Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons File Management Press this 1con to automatically save the experiment file Press this 1con to open a previously saved experiment file Press this 1con to start a new experiment Artisan Technology Grou
25. Ignore 1st coefficents E W Enable Truncate at channel H o I Enable e Set various parameters to automatically reject data The SOS limit will be exceeded when the monomodal fit 1s poor usually due to a multi modal size distribution The baseline limit will be exceeded if dust or bubbles are present The amplitude will be low if the S N ratio is poor usually due to low sample concentration Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings Data Filtering The Data Filtering allows Annotations Hardware Config Hardware Settings different data analysis options Data Filtering Solvent M Model Conditions Data Marking Measurements will be marked if they meet any of the criteria e Over SOS limit Rej ects data with subsequent ta monamadal cululants analysis higher errors a Eni Under Baseline limit 0 35 Enable e Under Over Baseline R ej ects Over Baseline limit 1 05 Enable pn E data with lower higher baseline rm Under Amplitude rejects data Ignore 1st coefficents Iw Enable with lower amplitude EN Truncate at channel f zl I Enable Correlation Function rejects Cancel correlation function points outside the specified range Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings Annotations
26. Tools Options Overview Tools Analysis He Settings Options honest WAT tn pst E Number 20 Measurement Made i e I Show All Measurements Count Mode e Select the Tools Options menu to Select time or measurement number for the X axis units in count rate and measurement windows Set the widths of the count rate and measurement windows Set the duration at which DYNAMICS will indicate that no measurements have been obtained Set parameters for low quality data that may be a result of high noise dust etc Set parameters associated with the real time count rate warning diagnostics Chose the directory where DynaLS may be Chose to display the Tool bar or Status bar Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Options Graphs Options Graphs Timeouts Toolbars Baseline T aol Directorie e Set X axis Source to Time and view Rh or Counts versus the elapsed time of the experiment C Number Count Mode 20 Measurement Made i 2 I Show All Measurements 159 5 238 5 319 0 e Orset X Axis Source to sample Number and view Rh or Counts versus the number of measurements set the X Axis to display time in order to monitor and analyze real time events 1 2 3 4 5 6 7 amp M9 10 11 12 Artisan Technology Group Quality Instrumentation Guarant
27. al gt Settings Options Measurement um Scheduler Start i RN omen poo po semi Connect SS A95 WE ost Experiment Settings Data Filtering l Solvent hw Madel Conditions Hardware Contig Hardware Settings Edit Annotations Hast Unit Serial Humber 3851 OynaPra 99 vL Reduced 105 Correlator Fi Bea Flex 38 usb correlator Optics Block Selection MS s n 088 Model Mame Senal Mo Wavelen Angle Cw Count Flust MS Host Unit Type APD Bias Normal 110052 0 E n3 View and edit t Oe Bed g0 Cancel Tabbed Menu Data Filtering Solvent Parameters Molecular Weight Model Conditions concentration Annotations comments Hardware Configuration Hardware Settings acq time Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings Data Filtering Tools Analysis Hie E Ed Settings 7 Annotations Hardware Config Hardware Settings n ptians B a Data Filtering Solvent ld Model Conditions ud Data Marking Measurements will be marked if they meet any of the criteria Senet is subsequent to monomadal cululants analysis Over S05 limit Under Baseline limit o 333 Enable Over Baseline limit aU Enable Under Amplitude lirnit 0 02 Enable Correlation Function
28. ation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Communications The DynaPro relies on a USB link with the personal computer for receiving internal software programs receiving parameters related to data processing and sending autocorrelation results Temperature control is communicated through a serial cable connecting the optics module with temperature control and the PC Communication errors are generally a result of cables not connected correctly or instruments not receiving power to respond Check cable connection and power to all instruments Check settings in the hardware configuration Tools Settings Hardware Config the host unit type must be set correctly Call Technical Support 1f 1t still does not connect Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com A rtisan Artisan Technology Group is your source for quality Pee new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment A EQUIPMENT DEMOS HUNDREDS OF Instral e REMOTE INSPECTION LOOKING FOR MORE INFORMATION TO qii Remotely inspect equipment before pu
29. ave data exp extension Save As save data in exp or tdf format with same or different file name Print create and print a semi custom graphical report Print Setup select and configure the printer for DYNAMICS output Export select and export measurement data File List quick access to the last four saved experiments Exit quit DYNAMICS Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com File Print Setup S Latus Default printer Ready Type RICOH Aficio 200 250 PCL Where Lab acer sricah Comment Onentation f Portrait C Landscape Paper SIZE Letter 5 5811 in E Source Auto Switching E cancel Before Printing make sure your printer 1s installed and selected Chose paper Orientation typically portrait and size Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com File Pr Printing Options xX v Annotations Results Summary Parameter Settings M Cumulants Catalog Regularization Datalog int Cancel Count Rate amp Measurement Graphs Bimodal Scatter Diagram Regularization Histogram Cumulants Histogram Autocorrelation Coeffs v eunen Meas Omp TEegsunemens iv eurent heas niu C AlliMedsurements Plume Regression Emor Create semi custom graphical and tabular reports Using your mouse check the i
30. e Quickly calculated estimated MW from Rh Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Calculators Concentration Minimum Concentration Calculator Analysis Help Coethicients il E an lol Enter Molecular weight KDa 14 7 L umulants Mag a Min Required Concentration Dynals 5 Select DynaPro Model 0 0953 mg ml ic M M M52 Static M Model 1 M Calculators Min Concentration LSH 6 482 UU tial Ratio chabert wt vol Recalculate All US z 0 00953 Apparent Fraction inmum Aequired Concentration mg ml 0 0953 Molecular eight Enter the MW of the biological particle that you will measure and the model of your DynaPro instrument The software calculates the approximate minimum concentration required for measurement e The results are displayed in various units mg ml moles per liter and wt Volume Entera value in the right hand column and convert Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Calculators axial Ratio Analysis Help Axial Ratio Elliptical Shape Calculator Coefficients ail E iis 017 Cumulants d Me Regularization me Enter Data Calculated values Dynal Ril 2 D i NN c te Og ce eee Molecular weight kD al g Frictional Ratio fi yf Static Mw Model TS M Specific Yolume ml g
31. eed 888 88 SOURCE www artisantg com Tools Options Graph width a Options ES EMEN c V DE Graphs Timeouts Toolbars Baseline Tal Directories Settings Options SHE ONET Number Eanmect iaa Gates Count Made 20 Measurement Made f B Show All Measurements ee o e Control the amount of information the number of measurements to be presented in the count rate or measurement windows Enter the number and press OK or select Show All Measurements Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Options DynaLS Directory Tools Analysis gH Settings Graphs Timeouts Toolbars Baseline Tol Directories NOTE Options ecifiy the directory in which the program file 1 rd Scheduler eae SUR a ee ade Ed DynaLs IS d 3 party EM Gdmad analysis algorithm available Hien ste aa exclusively through Protein solutions Contact our sales department if interested in a quote for the add in package cael Heb Unless otherwise directed DYNAMICS assumes that DynaLs 1s located in c DynaLs If you have installed DynaLsS into a different directory please specify its location using this menu command Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Options Toolbars Tools Analysis He Settings
32. l ESI tlt 0470 Curnulants e Show Bimodal switches the active windows between the results from the monomodal fit and Show Bimadal Result J Regularization Mark as Excluded the bimodal fit Dynals E T T A SEO Shom All M dal aiaee e For example use Show Bimodal to compare the MEE Histegrem monomodal histogram with the bimodal histogram vo IE of a measurement as shown below EM MN SD Scatter Diagram Regression Fit Error Radius nm Mw kDa Polyd frm of M R Radius nm MM kDa Fold nm of M A fieis fass p5 ao E pss he TE Fold Index o 08 17 00 HE 8 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Mark as Excluded is Hel Analysis IAE Cumulants DataLog 32nmstd exp OF x Coefficients T Il T A 10 Msrit Time s Ampl Di i Polydlndx Mass Temp C Count Rate Baseline Sos Error M D iili 1 100 0693 125 1538 amp 1 0 14 25533 100 192 904469 1 001 11458 2 200 0710 126 1626 30 0 03 25078 100 192 8897933 1 002 7 058 umulants Show Bimadal FH ezult 3 31 0 0710 128 16 07 37 0 05 2438 4 100 192 895332 0 998 3 369 MEME ees 4 41 0 0711 127 16 16 45 0 08 24688 100 192 887981 1 001 4482 Regularization k Mark as Excluded oes Sa OE CRE RIES ESC Mono 0 706 126 16 22 4 3 0 08 2492 1 100 192 8943933 1 001 6 594 Liv
33. n Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Regression Fit Error HMonomodal Error Meas 7 JEDE x 7 7 d EE Fit Error 0 005335 1 PB Time us 3 839399 EXT Ie H5 zs gt Coefficients i E ilin off L umulants Show Bimodal Result Regularization Mark as Excluded Diunials EN NR E Show All M anemaodal Static MV Model Show All Bimadal Calculators k Histogram Recalculate All Datalog Scatter Diagram Regression Fit Error v Time f Sep Close Regression Fit Error shows the residual error plot for each delay time e Parametric Fit Error Actual Coefficient Fitted Coefficient Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Regularization e Use this menu item to lt r e View the size distribution Analysis Help Coefficients El ae Histogram calculated from the PULLS Measuremer Regularization Algorithm Diass Daeg Phim e View the Datalog showing the aes is numerical results of the Recaleulateal LU p Regularization Algorithm Model Also available as an Icon e fit to a model of hard spheres e fit to a model of coils for polymers Dem rj wes ule mJ Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Regularization His
34. n Fit e Bimodal Two peak Size Distribution Fit e Regularization Laplace Inversion non negative least squares fit e In addition DYNAMICS offers an automatic interface to DynaLS The Monomodal and Bimodal algorithms operate on each set of autocorrelation coefficients calculated by the DynaPro and contained in the experiment file The Regularization algorithm fits the average of the autocorrelation coefficients of the unmarked measurements within the experiment file The reason for this 1s to assure the highest level of statistical accuracy for samples containing multiple species Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Algorithms Monomodal TEET w The Monomodal Algorithm models the size Channel e e calc Er Tr distribution as a Gaussian function defined by a Time us 0 357892 rel g mean radius and standard deviation The mean and standard deviation that minimize the parametric fit error fitted actual are defined and presented as the best fit DeD 108400 1081 10842 10 43 10804 1045 10806 10e Monomodal Histogram Meastt 1 Radius nm MM kDa Fold nm of M A 15 38 2553 3 E 37 0 Fold Index o1 4 e Refer to the theory of operation in the user manual for a more complete discussion Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Algo
35. nals Bi 1 0 202 286 8 58 ve ve 700 9 74 192 894393 1001 2123 Show All Monomodal Bi 2 0 509 107 1867 41837 2 Static Iw Model Show All Bimadal Calculators Histogram Recalculate All Datalog All Mono All Bi Close Scatter Diagram uu Regression Fit Error Cumulants DataLog 32nmstd exp IDE XI 113 1 F Msrit Time s Ampl Diff Radiusinm Polydinm Polydind MwiKDa Mass Temp C Count Rate Baseline Sos Error Mark specific outliers and exclude the oo f h 2 200 0710 126 1626 30 0 03 25078 100 192 889793 1 002 7 058 3 3 0 070 128 16 07 37 0 05 24384 100 192 895332 0999 3369 measurements rom t C average Va ue 4 410 O711 127 1616 45 0 08 24588 100 192 887981 1001 4492 Regularization and DynaLS Aves Mono 0 711 127 16 16 37 0 06 247117 100 192 891035 1001 43973 Bi 0196 314 809 i se 5735 7 192 891035 1001 1922 calculations Bi 2 0 519 109 1951 41737 28 indicates the point is marked Press Mark to mark press again to Unmark Mak _AllMono aigi Use the Ctr and Shift keys to highlight multiple rows Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Cumulants Show All Monomodal Analysis Help Coetficien Cumulants ts o Regularization Diunals Static Mw Model Calculators Hecalcula VM te All ils ESI 0371 Shaw Bimadal Result
36. ndividual tables graphs and data displayed in Dynamics that you want printed to your laser printer Data are printed in the order shown in the dialog box Refer to the documentation provided with your instrument for examples of the report Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com File Export Jv Annotations Results Summary Cancel M Parameter Settings Data Iv Analysis Results Curent Measurement Onl C Al Measurements Iw Autocorrelation Coefficients Format Ascii Text tab delimited W Include Headers Comma Delimited Export information for further analysis semi custom graphical and tabular reports Format Data is exported in ASCII format with columns separated by tabs or commas Headers Include or exclude the names of data columns select no if then importing to Excel Data Export analysis results and or coefficients for the selected measurement or all data Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com File Export Save As ea Save 3 JAE ga Wintel Juss 9 E EE Windows E Metlog tst J Vinmcad A Resetlog tet H File name xt nave as type Text Files txt Lancel e After selecting the information you wish to export press OK A dialog box will prompt you to name the file The file will be
37. nteed 888 88 SOURCE www artisantg com Menu Analysis bsa unfiltered exp Dynamics Demwens C Lau EEN n oia Cumulants i INENCEDESCNUNEGRUUUANERU E Saas pe pe a SA RE ER EOE Regularization E E tart f Cow Bunals Rhinmy 22862 242 93 C Tem Static MW Model MwikDak is28584 1925727 sv Calculators P Recalculate All Calculators 0r l 2 I Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Overview Analysis Help Loethicients Lumulants Regularization L unals Static Mw Model Calculators Recalculate All Use the Analysis Menu to View the autocorrelation coefficients calculated by the DynaPro Analyze results of the Cumulants algorithms Analyze results of the Regularization algorithm Analyze results of the DynaLS algorithm 1f installed Obtain MW and 2nd Virial Coefficient Access calculators for minimum concentration axial ratio MW and fraction calculations Recalculate all monomodal bimodal and regularization algorithms Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Coefficients mid e View the autocorrelation LOETTICIENLES mm coefficients calculated by the mu Autocrl Coef s Heast 1 Hp x Regu EASELS ECCLE DynaPro log normal plot Dynal Corl Coet 1 6937 r
38. ntg com Installation e Install DYNAMICS as follows e Insert the enclosed CD ROM into the CD ROM drive The software installation should start automatically In case 1t does not start e Select the Start button s located in lower left hand corner of the Personal Computer screen e Select Run from the menu type dAsetup and click OK Type the name of a program folder document or Internet zal resource and Windows will open it For you Open EMES Cancel Browse Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Installation Upon successful installation the DYNAMICS icon will become visible The software 1s now ready for use e Click on this IB icon DYNAMICS If DYNAMICS starts continue with QuickStart e If DYNAMICS does not start see Troubleshooting or call Technical Support for assistance Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Connect These instructions assume DYNAMICS is successfully installed on your computer and currently active e Connect the USB Cable to PC COM of the DynaPro host unit and Personal Computer Connect the two cables from the MicroSampler to the appropriate receptacles on the DynaPro host unit for temperature controlled models there 1s one additional 9 pin serial cable connected directly to a computer serial port
39. ollection analysis presentation and printing e DYNAMICS works best with a Pentium computer operating on Windows 98 with a color monitor and at least I16Mb of RAM e This manual fully describes the installation and operation of DYNAMICS version 5 software e Because DYNAMICS is an evolving software package you may notice some differences in the screen shots presented here and those from the latest version of DYNAMICS The differences are mostly visual Should you have any questions please contact our technical support team see following page and they will be happy to assist Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Table of Contents Technical Support e Installation e QuickStart e Menu Items e File Tools Analysis Help DYNAMICS Icons Algorithms DynaLS Troubleshooting Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Technical Support If for any reason you should experience difficulties with or have any questions pertaining to DYNAMICS please contact us at PROTEINSOLUTIONS 750 Vassar Ave Lakewood NJ 08701 U S A WWW protein solutions com North America 1 732 367 1663 Europe 44 1494 44 66 52 Japan Rigaku International 81 425 45 8184 All other countries 1 732 367 1663 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisa
40. p Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons Autocorrelation Coefficients Autocrl Coets Heast 1 OF x Channel Corl Coet f SS E Time ps 0 957892 EE 1 De HO0 1 De HI1 1 De HI2 1 Oe HIs 1 Oe H 4 1 De H5 1 De H6 1 Dej Press the Autocorrelation Icon to activate and view the Autocorrelation Coefficients associated with the active measurement Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons Cumulants Histogram HMonomodal Histogram Measi 1 na Radius nm Mw kDa Pod nm of MR 15 38 2553 3 61 37 0 Falud Indes jo 4 Press the Cumulants Histogram Icon to view the Monomodal or Bimodal Size Distribution Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Icons Cumulants DataLog Press the cumulants DataLog Icon to view the DataLog containing a summary of all measurements Cumulants DataLog S2nmstd exp Timels amp mpl Diff Fladius nm Feludinm Polydinds MwiKDal Mass Templ Count Rate Baseline Sos Error a 1 10 0 0693 125 15 38 b 1 0 14 2553 3 100 13 2 904469 1 001 11 458 20 0 0 710 125 16 26 3 0 0 03 2507 0 100 13 2 nod 9 5 1 002 058 3 31 0 0 710 128 16 0 3 0 05 2438 4 100 13 2 ass 0 999 3 369 4 47 0 D 711 12 16 16 45 0 08 2466 8 100 19 2 oor ga 1 001 4 492 AVES
41. rchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com 7 information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED ee Mus Contact us 888 88 SOURCE sales artisantg com www artisantg com
42. rithms Bimodal haol toen a EN zm e From the same autocorrelation coefficients the Corl Coe 1 5337 e e e e e e e ns pd Bimodal Algorithm finds the size distribution comprising two peaks that best fits the data associated with the specific measurement Bimodal Histogram Meastt 4 QeOl LDeHl 10e0 10e402 10e43 10e03 1305 106305 106307 Radius nm MY kDa Fold nm of M A 5 55 oo 1 17 00 2731 8 e Refer to the theory of operation in the user manual for a more complete discussion Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Algorithms Autocrl Coef s Heast 1 Conl Coet 11 6937 Channel 1 Time us 0 357892 Fal E see Dynamic Light Scattering Wyn Brown Clarendon Press Oxford University Press 1993 Chapter 4 Regularization From the AVERAGE of the autocorrelation coefficients from all of the unmarked measurements the Regularization Algorithm finds the size distribution that best fits the data without restrictions on the modality or shape of the distribution Regu Histogram Lys 004 exp OP Radius nm Fold nm Polwd Intensity Mass Peaks Sup 1st Peak 205 ps Fio foo 000 E Sup 2nd Peak C XMass Sup 3rd Peak Resolution p TIT HM A CLIE HE LUE C EDU LU U J l 10 0 100 1000 10000 Optimal Artisan Technology Group Quality Instrument
43. saved with the txt extension Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com File Multi Experiment Export Multi Experiment Export X Experiments Columns e After adding the Filename experiments you wish to New Open Save Saves export press OK e A dialog box will prompt you to name the file The file will be saved with the exg extension a The Columns tab allows elete per A prance UM to select which information 1s exported Experiment Group OK Cancel Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Menu Tools New Experiment Dynamics e e DESSEN Stt po Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Overview Tools Snalysis He e Use the Tools Menu item to control the experimental settings and data interpretation display including e Settings Experimental parameters criteria for data interpretation and annotations Options Graphical display timeouts e Scheduler allows unattended data acquisition Use Connect to manually load software or parameters into the DynaPro Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings Hew Expenment Dynamics cR cm pem em CE li I a 00700 al
44. sis Help Bee E deis aff dn IRER Count Rate 4 pr Measuremen Count n 3m Fih bud x E stat C Temp 0 0 joo RA MwikDak oa foo The following section describes DYNAMICS Menu Commands READY mS ad 24 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Menu File 32nmstd exp Dynamics File Tools Analysis Help Hew CiN moa l Open Lir ah j Save Ctrl S 4 r Measurement NNI NER peer i h a 1838 22 zei Enn sp MwikDa 25533 24s2 1 AT Short Help for the DYNAMICS Menu IE Commands appears 1n the status bar 25 eei patie status i x Change the printer and printing options MEME ae me Export 1 CATI SERpsS3Znmstd exp 2 LATAS bea untltered exp 3 CATIN S EspssBImadl esp Z TAS LIS ERpESUBUA84 7 exp E mit Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com File Overview 32nmztd exp Dynamics File Tools Analysis Help New Open Save Save 5s Print Print Setup Export 1 CATIN AB spss2rimestd esp 2 CATIN Absa unfiltered exp 3 CATIN SERBpeSBImadl esp 4 CATIA E ps sUBU434 7 exp E sit Ctrl tri Ctrl S New begin a new experiment Open open experiment files Save S
45. stPeak rata 73613 s25 Tr ic intensity E C XMass Sup 3rd Peak F Sup 1st Peak o m je i SI fess iy Sup 2ndPeak 134334 ooo po feso 33 eas Sup 3rd Peak teed 888 88 SOURCE www artisantg com Analysis Regularization Datalog Loa qM e View the detailed results of the regularization MEINE algorithm using the Datalog window D iA isl e Print or export the results from the File Print Menu Static Mw Model E Calculators Recalculate All Hadiusinm Di AS cather 5 8e 005 T 6e 007 4 1e 005 5 09 5 5e 007 18 0 E 0 00011 703 4e 007 0 0 0 0 Peak Means b ze o5 r 3e D07 100 0 100 0 Also available as an Icon esi La DE A Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Regularization Model 060494 7 exp Dynamics Mnl x File Tools Analysis Heb PRS ues aes a aT 1 j ES da oir12 The model biel Coefficients Count Ral L umulants Measurement l M E command fits the Cou URS vmm anus Rh nm 450 4 54 da t b mb Asien f Tem Static Mw Model d E Spheres E i173 IE sv fe hf e Calculators k the particles as hard spheres or Gaussian coils for polymers 150 Recalculate All 100 5n T 1 0 3 5 5 5 3 3 12 0 158 5 438 5 319 0 E Assume spherical molecules E
46. tion Guaranteed 888 88 SOURCE www artisantg com Tools Settings Solvent Annotations Hardware Contig Hardware Settings Data Filtering Solvent MwMaodel Conditions o Set DYNAMICS to reflect the Selection Aqueous Buffer i refractive index viscosity and Refract Inds Viscosity ViscTempCoet 14 Aqueous Buffer 1 333 1 019 224 temperature coefficient of the GI 10z 1 333 1 288 2024 Glycerol 20x 1 333 1 734 2024 solvent b uffer System Ethy Glycol 10 1 333 1 274 2024 Eth Glycol 20 1 333 1 658 2024 DYNAMICS IS provided with a Glucose 104 1 333 1 327 224 Glucose 20 1 333 1 9 2024 table of commonly used solvents Add or edit the table to Select Edt Dee meet your specific requirements OF Cancel Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings MW Model Standard Experiment Settings Annotations Hardware Config Hardware Settings Data Filtering Solvent Mw Model Conditions f Standard Curve M Ww RhFactarRh Power C Vol Shape Hydration MWz 4 3l PIN a Rh FricRatio aS pecol Selection Potens 00 Molecule Hh Factor _ zs ee Proteins eee NEN NM 2 33080 es Pullulans 4782 1 8136 Ficalls 1 3071 22713 endrimers 884 3 3838 Select Edt Delete OK Cancel Calculate MW from hydrodynam c radius using the log log relationship between MW and Rh developed b
47. togram Analysis Help Regu Histogram Lys 004_exp OR x Coefficients ail Eg ii Radius nm Fold nm 7 Fold Intensity Mass Peaks Sumate d Sup 1st Peak 2 05 0 15 1 100 0 100 0 genu Regularization Histogram Sup 2nd Peak hase Dynals n Datalog B Sup 3rd Peak Static hw Model Model k Calculators Recalculate All View the size distribution histogram calculated by the non negative least square error algorithm A TO A TAT CEUTA TTT 0 J l 10 100 1000 10000 The algorithm finds the best fit Optimal without using a specific size distribution model Also available as an Icon Provides mean radius value information for up to three peaks Use this algorithm to confirm size Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Analysis Regularization Resolution Slider If apriori information 1s available the resolution slider can be used to decrease the noise constraints in the algorithm and possibly resolve single peaks into multiple peaks The Auto Scale option can be used to change the scale of the Regularization histogram The Sup Peak options can be used to remove peaks from both the display and the Intensity amp Mass calculations Artisan Technology Group Quality Instrumentation Guaran Radius nm Fold nm Fold Int z Mass Peaks Su Sup i
48. umentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Data Interpretation bsa unfiltered exp Dynamics IDE x File Tools Analysis Help pem fo we fie ml fo lt gt Count Fate Avg LEE f Count 461 440 Regu Histogram bzaiunfiltered exp Pilimi Elle C Teme 250 f0 mass Scat Mas stMean 0 05 21 100 6 XS 500 and Mean 21 53 joo C Ma 3rd Mean 479 96 52 6 Jao j Measurement Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com In this sample the Regularization Algorithm does not show a monomodal distribution A solvent noise peak and large aggregates are present in the sample QuickStart Data Interpretation The Scatter Diagram is an X Y plot of all of the monomodal or bimodal results The bimodal data form a pattern that can help to identify whether the size distribution contains one or two peaks The monomodal algorithm quantifies the polydispersity of the size distribution The Regularization algorithm analyzes the autocorrelation data without restricting the shape or modality of the size distribution The algorithm displays the mean radius fraction of scattered light and fraction of mass of each component in the size distribution Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com QuickStart Saving Data
49. y PROTEIN SOLUTIONS Create your own MW models with the editor Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings MW Model Vol Shape Hydration Experiment Settings e e Alternatively calculate Annotations Hardware Contig Hardware Settings Data Filtering Solvent Mw Model Conditions f MW using partial specific C Standard Curve Iw z RhFactar Rh Power volumes and frictional f Yol Shape Hydration M 443FPI Ma RhzFricR atio 3 5 pecol i ratios e Add your own MW models with the editor Selection Lipase Molecule Mame pecite v alume Frictional A atia 4ve 24 1 10kD a 725 1 25707 Human Serum Alb 0 733 1 288 FSH Select Add Edit Delete _Select_ Edt _ Delete LIK Cancel Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Tools Settings Hardware Settings Acquisition Time Experiment Settings EJ Data Filtering Solent Mw Model LConcdiberis Annotations Hardware Contig Hardware Settings Sensitivity Acquisition Time sec 2 5 M Threshold 25 SPEM Temperature deg C 20 Communications Device Temperature Controller Settings Fort COMe Settings 2400 8 1 Cancel Control the amount of time the DynaPro acquires data Obtain data as quickly as 75 sec Use a shorter acquisition time 1f

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