BenchPro™ 4100 Card Processing Station
Contents
1. To save the updated custom protocol press the Save function key and enter the protocol name in the Save protocol screen using letters in the on screen keyboard with the arrow keys page 23 Press the Save function key to save the edited protocol under a new name The new protocol now appears on the Protocols screen Proceed to assembling the unit with reagents and card page 12 to perform the custom blotting protocol Continued on next page Programming a Custom Protocol Continued Creating a New 1 From the Protocols screen select New to create a new protocol Protocol 2 Select Insert function key to add steps or substeps Protocol Edit New Exit Insert Delete Save Run 00000 3 The default step name Wash and time 1 minute are displayed Edit parameters such as the step description and time for each step by using the Right Left arrow keys Protocol Detail View WesternBreeze 176 Min Exit Insert Delete Save Run Press the Substep function key to add a substep within a step After making the changes press the OK button to accept changes and allow navigation away from the edited step Continue repeating Steps 3 5 until all steps of the protocol are added To save the updated custom protocol press the Save function key and enter the protocol name in the Save protocol screen using letters in the on screen keyboard with the arrow keys page 23 8 Press the Save function key to2. 1 Ab 30 min Return 3 Wash 11 min 4 2 Ab 30 min 5 Wash 11 min Back Edit Half Edit Full Half Full 000009 t 5 The Run menu is displayed Proceed to assembling the unit with reagents and membrane as described on page 12 10 General Guidelines Introduction Recommended Cards Important General guidelines for using the BenchPro 4100 Card Processing Station with BenchPro 4100 Western Card are discussed below To use the BenchPro 4100 Card Processing Station for immunodetection you need to purchase the BenchPro 4100 Western Card from Invitrogen Ordering information is on page 33 Do not use any other cards with the unit Up to 4 BenchPro 4100 Western Cards can be used to process an identical number of membranes If loading fewer than 4 cards verify that the reagent bottles and vials are also loaded in the tray in an order corresponding to the cards The unit automatically detects which slots contain cards and only slots containing the cards function during processing Always load reagent bottles and vials in the reagent tray without caps to allow the Card Tips to draw the solution into the blot chamber Always load the reagent bottles and vials before inserting the card into the instrument The Blot Holder containing the membrane may be inserted into the card before or after the card is loaded into the unit To obtain the best results follow these recommendations Wear gloves lab
3. The BenchPro 4100 Card Processing Station is compatible for use with a variety of immunodetection reagents and protocols All commonly used immunodetection reagents are supported The reagents are added to the supplied reagent bottles and vials and placed in the reagent tray prior to use Continued on next page Overview Continued System Overview The BenchPro 4100 Card Processing Station performs automated reagent Features processing using electronic solenoid technology developed by Invitrogen To use the BenchPro system for immunodetection protocol you will 1 Perform western transfer of proteins to nitrocellulose or PVDF membrane using standard procedures 2 Assemble the reagent bottles and vials containing the required reagents for immunodetection and place the reagent bottles and vials on the reagent tray in the instrument 3 Insert the BenchPro 4100 Western Card into the card slot on the BenchPro 4100 Card Processing Station 4 Transfer the membrane to the Blot Holder which is then inserted into the BenchPro 4100 Western Card 5 Select and run the pre programmed immunodetection protocol of choice or program and run your own custom protocol During the automated immunodetection protocol reagents are pumped from reagent bottles and vials located in the reagent tray into the card and around the membrane The order of reagents used and timing of protocol steps are based on the selected protocol After eac
4. Upon Receiving the Instrument BenchPro 4100 Western Card The contents of the BenchPro 4100 Card Processing Station are listed below Component Quantity BenchPro 4100 Card Processing Station 1 Specific Power Cord 3 for U S Canada Taiwan Japan Europe and UK Reagent Bottles set 1 4 x 250 ml Reagent Bottles set 2 4 x 125 ml Reagent Vials 12 x 25 ml Drain tubing for auto drain with attached connector 6 feet length 1 Quick Reference Card ORC 1 Instruction Manual 1 See page 4 for specifications and description of the BenchPro 4100 Card Processing Station and page 8 to install the instrument Examine the unit carefully for any damage incurred during transit Any damage claims must be filed with the carrier The warranty does not cover in transit damage The BenchPro 4100 Western Card is used with the BenchPro 4100 Card Processing Station for western blotting application and is available separately from Invitrogen page 33 Product Catalog no BenchPro 4100 Western Card WP1001 The BenchPro 4100 Western Card box includes 10 BenchPro 4100 Western Cards and 10 Blot Holders Safety Information Safety Informational Symbols A Caution vi Follow the instructions in this section to ensure safe operation of the BenchPro 4100 Card Processing Station The BenchPro 4100 Card Processing Station is designed to meet EN61010 1 Safety Standards To ensure safe reliable operation always o
5. during processing Methods Getting Started Installing the BenchPro 4100 Card Processing Station Using BenchPro 4100 for the First Time 1 Unpack the BenchPro 4100 Card Processing Station as instructed on page viii 2 Three power cords are shipped with the instrument to ensure that the cord you use is compatible with your local socket format 3 Place the BenchPro 4100 Card Processing Station on a level laboratory bench Keep the area around the instrument clear to ensure proper ventilation of the unit 4 For your safety Position the instrument properly such that the power switch located on the instrument s left side and the AC inlet located on the rear of the unit page ix are easily accessible Be sure to position the instrument such that it is easy to disconnect the unit Ensure the AC power switch is in the Off position page ix Attach the power cord to the AC inlet and then to the electrical outlet Use only properly grounded AC outlets and power cords 7 Attach the 6 drain tubing supplied with the unit to the waste outlet if automatic waste draining is desired page 6 by pushing the quick connect fitting of the tubing into the hose connector of the auto drain outlet in the rear of the instrument Quick connect fitting of tubing Auto drain outlet You are ready to use the BenchPro 4100 Card Processing Station See page 11 for details If you are using the BenchPro 4100
6. Card Processing Station allows processing of up to four cards in parallel with up to four different reagent sets The BenchPro 4100 Card Processing Station automates routine manual processing steps for standard laboratory procedures such as western immunodetection while maintaining performance comparable to manual processing See page 4 for details on various parts of the system The BenchPro system consists of e BenchPro 4100 Card Processing Station The BenchPro 4100 Card Processing Station with BenchPro 4100 Western Cards allows automated processing of incubation and washing steps for up to four membranes at a time The instrument is a simple user friendly benchtop unit that is preprogrammed with two WesternBreeze immunodetection protocols and one standard ECL protocol See page 4 for details e BenchPro 4100 Western Card The BenchPro Card is a disposable card that is inserted into the slot on top of the BenchPro 4100 Card Processing Station The card holds the membrane to be processed in a Blot Holder During operation reagents are pumped from reagent bottles and vials located in the reagent tray into the card and around the membrane The order of reagents used and timing of protocol steps are based on the protocol selected e Reagents not supplied with the instrument Currently the BenchPro 4100 Card Processing Station is available for immunodetection applications using the BenchPro 4100 Western Card
7. Card Processing Station for the first time e Unpack the reagents bottles and vials from the package and store the bottles and vials e You may wish to rinse the Waste Basin Reagent Tray Top and Bottom and Clean assist attachment with water before use see page 22 for cleaning and maintenance of parts Be sure to place all parts back properly in the instrument before use e Review the preprogrammed protocols to ensure that one of the protocols meets your requirements page 9 or program a custom protocol page 23 Continued on next page Getting Started Continued Selecting a Preprogrammed Protocol You need to select an appropriate protocol on the BenchPro 4100 Card Processing Station prior to assembling the unit with the transfer membrane and reagents To create a custom protocol see page 23 1 Press the power switch located on the left side of the unit page ix to turn ON the BenchPro 4100 Card Processing Station The Power status light turns green and the instrument performs a self test of electrical and pneumatic systems Once the status check is complete air pressure and vacuum reach nominal values as indicated on the display the Protocols screen is displayed Select the appropriate preprogrammed immunodetection program by using the Up down arrow keys to move cursor to the program and then press OK Choose WesternBreeze 95 min for a faster WesternBreeze immunodetection protocol wherein the incubatio
8. The maximum on board volume for a particular reagent has been reached Reduce the number of steps or substeps Used card s in slot x Replace with unused new cards and press OK OG Cannot begin a A step must first be entered to enter a substep protocol with a substep Exceeded max steps The maximum number of all steps and substeps in substeps a protocol is 20 The max number of Cannot add more than 6 substeps to a step substeps is six Please save protocol Save a new or edited protocol before you can run before running the protocol Please save to valid A name was not selected for a new or edited protocol name protocol A protocol cannot be named New You are adding a The volumes in the 25 ml tubes used for repeat AB or Block antibodies and blocker do not allow for more than one use per protocol You cannot add more than one step for block primary antibody and secondary antibody step within a protocol 30 Appendix Replacing the Fuse Replacing the Fuse For additional fuses contact Technical Support page 33 Instructions are provided below to replace the 250 V 1 25 A fuse for the main power socket 1 Turn off the main power switch at the left side of the instrument and detach the power cord from the rear of the instrument 2 Open the fuse compartment located on the rear of the instrument using a small flat blade screwdriver to gently pry open the fuse compartment 3 Pull the fuse holder o
9. environmental agency for disposal regulations Proceed to Loading the Card next page Continued on next page Using the BenchPro 4100 Card Processing Station Continued Loading the Card Before loading the card and membrane make sure you have selected the correct protocol or programmed a custom protocol Be sure to set up reagents in the reagent tray to start the protocol immediately once the membrane is placed in the card to prevent the membrane from drying during reagent or protocol setup Note You can run up to 4 cards using different reagent sets but using the same protocol 1 Remove the required number of BenchPro 4100 Western Cards and Blot Holders from the box Always hold the card near the finger grip area at the top of the card Do not hold the card by card tips q il 2a De AAA Al P _ i 2 Remove the card from the package and insert the card into a slot in the BenchPro 4100 Card Processing Station such that the tips are inserted into the reagent bottles and vials placed in the reagent tray Use the Card Alignment Guide on the side of the slot to ensure the card is placed correctly When the card is placed correctly in the slot you will hear a hard click Note There should not be any resistance to card insertion If you feel significant resistance withdraw the card reposition and reinsert Fr Na BTN 3 After transfer wash the tr
10. is used Continued on next page 11 Using the BenchPro 4100 Card Processing Station Introduction Instructions are provided in this section to use the BenchPro 4100 Card Processing Station with the BenchPro 4100 Western Card for automated processing of the transfer membrane Materials Needed You will need the following items Ordering information is on page 33 e _BenchPro 4100 Western Card and Blot Holder e Reagents blocking buffer washing buffer primary and secondary antibody e Detection reagent chromogenic or chemiluminescent substrate e Nitrocellulose or PVDF transfer membranes containing blotted proteins Preparing The BenchPro 4100 Card Processing Station is compatible with a variety of Reagents immunodetection protocols and reagents Reagents for WesternBreeze immunodetection as well as alkaline phosphatase and horse radish peroxidase HRP chromogenic and chemiluminescent substrates are available separately from Invitrogen page 33 1 Prepare the reagents used for your immunodetection protocol as you would using manual processing or as directed by the reagent manufacturer Prepare reagents directly in the calibrated reagent bottles and vials supplied with the unit or prepare reagents separately and transfer the required amounts into the supplied reagent bottles and vials Typical reagent volumes for one run are listed below Reagent Half Blot Full Blot Maximum Vol Allowed Blocking solution 13 ml
11. pumps air into the card for 30 seconds the card is rendered unusable The instrument color sensor does not record the color change and discontinue the run Before starting the run always ensure that reagents bottles are loaded with the respective reagents Continued on next page 17 Using the BenchPro 4100 Card Processing Station Continued Running a Preprogrammed Protocol Continued 18 The instrument starts the protocol run the status light changes to green and you will start to see the solution being pumped into the card chamber Note The instrument performs process checks in the first few minutes to ensure proper functioning and any errors if detected are displayed in the display screen Refer to page 30 for a list of error messages The instrument is designed to run only new unused cards If any used cards are accidentally used the instrument detects used cards and displays a used card error message page 30 While the protocol is running various steps of the protocol are displayed on the screen with the time remaining for each step substep as well as the total protocol time remaining Note The times displayed are for incubation times only and do not include time required for filling the card and empty cycles WesternBreeze 95 min Total Step Time 1 min Cycling Sub Step Time Remaining 37 Total Step Time Remainina 37 During the run the Abort function key or Pause function key can be used to interrup
12. run Procedure 20 To manually interrupt the protocol press the Pause function key Using the Pause function key stops both the protocol and the timer Note The Pause key can be used to stop the protocol in order to remove the membrane The card itself cannot be removed from the device so use forceps to remove the Blot Holder containing the membrane For convenience we recommend doing this between steps when the card is drained of liquid Press the OK button on the directional pad to restart the protocol from the point from which the protocol was paused To manually abort the protocol press the Abort function key Note Aborting the protocol stops instrument operation immediately After an abort reagents pumped out of the cards to the waste containers To restart after an abort check the reagents or remake reagents because the reagent volumes may have changed or the reagent may be diluted from the reagent in the card if the reagent was recycled to the reagent bottle at the time of the abort step Use a new card after the abort especially if reagents are pumped into the card Press the Run function key to restart the protocol from the beginning or the Back function key to return to the Protocols screen To obtain best results remove the membrane from the card within a few minutes of ending the procedure and draining reagents from the cards 1 Carefully remove the Blot Holder from the card and then remove the membrane from
13. save the edited protocol under a new name The new protocol now appears on the Protocols screen 9 Proceed to assembling the unit with reagents and card page 12 to perform the custom blotting protocol 27 Troubleshooting Introduction Review the information below to troubleshoot your experiments using the BenchPro 4100 Card Processing Station with BenchPro Western Card No power the AC power cord is not Check AC power cord connections at both ends Use the digital display connected correct cords remains blank Fuse has blown Replace the fuse page 31 and the pump does not run when the power is turned on Error message Refer to the list of error messages on page 30 displayed Accidentally Be sure to confirm that you have added the card reagents missed adding and reagent bottles in the correct order prior to starting the card reagents protocol If the run is aborted after the run has begun you or reagent need to restart the run Do not reuse the card if the run is bottles aborted within a few minutes of starting the run If the problem still persists after verifying that correct power cord is used and the fuse is replaced contact Technical Support page 33 Card is difficult Bottles have capsor Make sure that the reagent bottles and vials are uncapped to insert card is not oriented when placed in the reagent tray correctly Insert the card into a slot in the BenchPro 4100 Card Processing St
14. the BenchPro 4100 Card Processing Station Continued Running a Three immunodetection protocols are preprogrammed into the unit and Preprogrammed cannot be deleted or over written by the user as indicated by the lock placed Protocol next to protocol names on the Protocols screen If you have already preprogrammed a custom protocol then you can use it at this time To program a custom protocol see next page 1 If you have already selected the protocol and the half or full blot option proceed to Step 5 Select a preprogrammed protocol from the Protocols screen using the arrow keys page 9 then press the OK button The Protocol Detail View screen is displayed Verify the protocol using arrow keys Select the option to run a protocol for half or full membrane by pressing the Half or Full function keys Note When the Half option is selected the card blot chamber is filled approximately half full with reagents allowing the use of smaller reagent volumes when small or half size blots are processed The Run screen is displayed Ensure that the cards are loaded with the membrane and reagents are loaded in the reagent tray before pressing Run to start the protocol WesternBreeze 95 min Half Verify Reagents are Loaded 00000 Press the Run function key to begin the run or the Back function key to return to the Protocols screen Important Do not run unused cards without loading reagents into the tray If the instrument
15. the holder using forceps If desired after removal place the membrane in water until you are ready to perform detection steps Proceed to the detection step For example for chromogenic detection place the membrane in a container containing the substrate solution and incubate until the signal is observed Remove and discard the cards appropriately Do not reuse the cards The instrument detects used card by a color indicator spot located at the lower right hand corner of the card which turns red when the card is used Continued on next page Using the BenchPro 4100 Card Processing Station Continued Post run Procedure Continued 6 i Open the Reagent Drawer Unit and remove the reagent tray assembly Dispose of any unused reagents in the bottles and vials appropriately Optionally use the Clean assist attachment to empty all reagent bottles at once without removing individual bottles To do this lift the Reagent Tray assembly upper and lower Reagent Tray pieces with loaded reagent bottles and vials away from the Waste Basin then place the Clean assist attachment on top of the bottles and vials while they are still positioned in the Reagent Tray While holding the assembly together invert over a sink to discard all reagents at once Note The weight of a fully loaded reagent tray and waste tray trays bottles and solution is 3 kg or 6 6 pounds at AA afam T Remove the Waste Basin and discard the wa
16. 18 ml 25 ml Primary antibody in diluent 13 ml 18 ml 25 ml Secondary antibody in diluent 13 ml 18 ml 25 ml Wash buffer Depends on the 250 ml protocol used Deionized water Rinse Depends on the 125 ml protocol used To the required volume of reagent needed add an additional 10 ml volume to the wash and rinse bottles to allow for unusable residual liquid that the card tips cannot reach For example if the required volume is 150 ml add at least 160 ml of Wash buffer or Rinse to the bottle 2 Add the reagents to the appropriate container as listed below 250 ml Reagent bottle Wash buffer 25 ml Reagent vial A Blocking solution 25 ml Reagent vial B Primary Antibody 25 ml Reagent vial C Secondary Antibody 125 ml Reagent Bottle Deionized water Rinse 3 Proceed to Assembling the Reagent Tray Assembly next page 12 Using the BenchPro 4100 Card Processing Station Continued Assembling the Instructions are provided below to assemble the Reagent Tray Assembly with Reagent Tray reagent bottles and vials containing the required reagents Assembly 1 Open the drawer unit and place the waste basin into the drawer Tray top pmen bottom 3 Assemble the reagent bottles and vials containing reagents in the correct container without caps in the Reagent Tray Bottom Vials will insert through the Tray Top and seat in the Tray Bottom Start placing the reagent bottles from the rear of the tray in the order as shown below and
17. Card in the supplied box to prevent any damage to the card To avoid damaging the card e Do not drop the card or bend card tips e Always handle the card from the top side away from the card tips A finger grip area is located at the upper left of the card to aid with handling e Do not manually add any solution to the blot chamber Note If you accidentally damaged the BenchPro 4100 Western Card you can purchase additional cards from Invitrogen page 33 Finger grip area Blot Chamber Sealing gaskets Card Alignment Guide Card Tips Blot Chamber The blot chamber holds the transfer membrane one full mini blot or one half mini blot During the immunodetection procedure various reagents as specified by the protocol are channeled into the chamber for incubation with the transfer membrane The blot chamber is designed to allow the membrane to be completely immersed in solution during all steps of the protocol Card Tips The Card Tips are plastic tips that are attached to the card and inserted into the reagent bottles and vials in the reagent tray During the procedure the tips aspirate reagents from containers in the tray into the blot chamber and are also used to return reagents back into the same vials after use and direct reagents into the waste basin Card Alignment Guide The Card Alignment Guide aids with the proper positioning of the card into the slot so the tips are aligned correctly into the reag
18. Invitrogen by technologies BenchPro 4100 Card Processing Station For automated processing of routine washing and reagent incubation steps of standard laboratory procedures Catalog no WP0001 Rev Date 9 September 2010 Part no 25 1049 MANO0001752 Contents Product Contents roa stated arden a dean daden A adahndenehaeh zalde Vv Sate ONO ensen antenne danae ede dansende taan EN vi Productopeciican NS enorme endet E N EEO vii Unpacking the BenchPro 4100 Card Processing Station nnee enenenvenenvenenvenenvenenvenenns vili benchPro 100 afd Processing Stat OM es snoncrnbinedoenereae ee r aaen Ehe ran EEE EER EEES ix SEO UCT OMN Ernane E E A 1 OA A 1 Desenphon of Barts added edet dende ahaa oat nasatuans 4 IICUNO OS EO 8 Cetine Sted icons aap aes enen T ant le call era ies aco anucc a 8 General GiideneS sanne a anekdote 11 Using the BenchPro 4100 Card Processing Station nternet adeleae eden 12 Programmis a Custom Protocols en Ea EN A 23 Trouble hoon ssd deden enden benenden 28 BenchPro 4100 Card Processing Station Error Messages ann enenveneenenvensenenvensenenvenvenenvn 30 Jao 01 19 0 DA 31 Kepl cme the BU Se iss nonnen daddeh oneens 31 Repackae mie the Ins trun Enneberg 32 Accessory Prodi anas es a sal eaniendetslg eal venders ia ental uaa rg ete eng edeee ee 33 Techical SU DOLL esin A 34 Purchaser Noti Cat OM aipa S Saat E T T TN 35 iii 1V Product Contents BenchPro 4100 Card Processing Station
19. Processing Station are shown below The USB A port bottom is used to connect a USB memory drive and the USB B port top is used to connect to a personal computer The AC inlet is to connect to the power outlet on the wall and the auto drain fitting is to connect the drain hose quick disconnect fitting to the unit Side View Rear View AC inlet Power switch Auto drain Continued on next page IX BenchPro 4100 Card Processing Station continued User Interface The user interface of the BenchPro 4100 Card Processing Station consists of e The Digital Display that shows the protocol that is in use and various steps of the protocol e Power and Status lights indicate the status of the run or error e Various function keys that are used to enter parameters and operate the instrument e Directional arrow keys allow you to navigate menus and select protocols or steps during instrument setup and operation BenchPro 4100 Digital display invitrogen Looking for Pressure and Vacuum Pressure O kPa mmm WS OO wr OOOO Overview Introduction System Components Introduction The BenchPro 4100 Card Processing Station is an automated benchtop device that uses electronic solenoid technology to control the transfer of on board compressed air and vacuum to a disposable card for automated fast and reproducible processing of routine steps for standard laboratory procedures The BenchPro 4100
20. akly immunodetection bound proteins from membrane after Non specific binding on the blot blotting Short blocking time Make sure that each step is performed for the specified or long washing time amount of time Affinity of the Check with protein standard manufacturer for homologies primary antibody for with primary antibody the protein standards Membrane is Wear clean gloves at all times and use forceps when handling contaminated by membranes Always handle membranes around the edges fingerprints or keratin proteins 29 BenchPro 4100 Card Processing Station Error Messages Introduction This section describes the error messages displayed Most of the error messages are self explanatory and after fixing the error you should be able to continue with the protocol Contact Technical Support page 33 if you need to send the instrument for servicing Error Message Action Drawer open close The reagent drawer is open Make sure the drawer before running is completely closed before continuing Waste detected empty Waste reagents are detected in the waste tray before running Empty the waste tray or attach the drain tubing before starting a protocol A leak has been An air or vacuum leak has been detected in the detected in the system system Call Technical Support page 33 No card detected A protocol was started without a card inserted into any slot Insert a card and restart the protocol Reached max volume
21. ansfer membrane briefly in water in a tray to remove any gel and transfer buffer components If you are using washed and dried membranes e Wash nitrocellulose membrane with water for 5 minutes twice in a tray proceed to Step 4 e Re wet the PVDF membrane in methanol in a tray wash the membrane with water for 5 minutes twice and then proceed to Step 4 Note Use wet or dry nitrocellulose membranes but only use wet PVDF membranes Wetting the membrane before insertion into the instrument results in more even blocking as sometimes dry nitrocellulose can wet unevenly and slowly in places resulting in suboptimal blocking thereby affecting detection Continued on next page 15 Using the BenchPro 4100 Card Processing Station Continued Loading the Card 4 Open the Blot Holder by slightly spreading the halves such that the Blot Continued Holder remains open when released to allow easy membrane insertion 5 Using forceps place the transfer membrane in Blot Holder Position one edge of the membrane toward the bottom of the holder making sure that no portion of the blot extends outside the holder 6 Holding the sides of the Blot Holder together slide the Blot Holder containing the membrane into the blot chamber of the card Transfer membrane insertion is easier if the card is already inserted into the instrument 7 Proceed immediately to Running a Preprogrammed Protocol next page Continued on next page 16 Using
22. as labeled on the reagent tray a 250 ml Reagent bottle contains wash buffer b 25 ml Reagent vial contains blocking solution c 25 ml Reagent vial contains primary antibody d 25 ml Reagent vial contains secondary antibody e 125 ml Reagent bottle contains deionized water rinse Note You can use 1 4 cards depending on the number of samples that you wish to process If you are loading less than 4 cards ensure that the reagent bottles and vials are positioned in the reagent tray rows that correspond to the slots used by the cards Continued on next page 13 Using the BenchPro 4100 Card Processing Station Continued Assembling the Reagent Tray Assembly Continued 14 4 Optional If you plan to use the Clean assist attachment you can attach the Clean assist attachment to the Reagent tray top as shown below Clean assist attachment Place the reagent tray assembly within the Waste Basin Close the Reagent Drawer unit completely If you are using the auto drain feature connect the 6 plastic tubing provided to the hose connector at the back of the unit as described on page 8 For proper draining of waste maintain the tubing at a height lower than that of the hose connector If the waste can be poured directly in the sink then position the hose outlet in the sink If the waste cannot be poured directly in the sink position the hose outlet in an appropriate waste container Consult with your local safety and
23. ation such that the tips are inserted into the reagent bottles and vials placed in the reagent tray Use the Card Alignment Guide to ensure the card is placed correctly in the slot When the card is inserted correctly in the slot you will hear a hard click Weak or no Detection step missed The detection step is not included in the instrument protocol signal from the or detection reagents After the instrument protocol is complete perform the blot not working detection step using your standard detection reagents and protocol manually Make sure the detection reagents are functional Insufficient Remove blot from detection reagent when signal to noise incubation with ratio is acceptable detection reagent Incorrect order of Be sure to add the reagents in the order described on page 13 reagents added to ensure all steps are performed correctly Make sure transfer apparatus and membrane sandwiches are Poor or incomplete transfer assembled correctly Use appropriate transfer times After blotting stain membrane to measure transfer efficiency Protein of interest ran Use positive control and or molecular weight marker to off the gel match gel separation range to size of protein being blotted After blotting stain membrane to measure transfer efficiency Continued on next page 28 Troubleshooting Continued Weak or no Sample too dilute Load a larger amount of protein onto the gel signal from the Poor retentio
24. cking and installing the BenchPro 4100 Card Processing Station e Operating the BenchPro 4100 Card Processing Station with a disposable BenchPro 4100 Western Card e Cleaning and maintaining the BenchPro 4100 Card Processing Station e Troubleshooting Description of Parts Introduction BenchPro 4100 Card Processing Station The various parts of the BenchPro 4100 Card Processing Station and BenchPro 4100 Western Card are described below The BenchPro 4100 Card Processing Station is a benchtop automated processing unit capable of processing 1 4 experimental samples The BenchPro 4100 Card Processing Station when used with a BenchPro 4100 Western Card delivers the reagents from the reagent tray to the sample for processing based on the selected protocol The BenchPro 4100 Card Processing Station and Western Card are designed to accommodate overnight protocols of up to 19 hours of continuous operation See page ix for a front and rear view of the device The front view of a BenchPro 4100 Card Processing Station identifying various parts is shown below 4 Card slots Digital display and Function keys Reagent drawer Reagent Drawer Unit The Reagent Drawer Unit contains the reagent tray assembly which holds vials of reagents for use with the desired protocol a clean assist attachment designed to streamline reagent cleaning and a Waste Basin used to collect waste solutions page 6 for deta
25. ent bottles vials Continued on next page Description of Parts Continued Reagent Drawer The Reagent Drawer Unit contains the Reagent tray assembly Clean assist Unit attachment and Waste Basin The Reagent Tray Waste Basin and one set of reagent bottles and vials are included with the instrument Upon receipt store the reagent bottles and vials in a cool dry place The Reagent Tray Assembly is composed of e Waste Basin is used to collect the waste during the protocol and contains a waste drain valve to drain the waste e Reagent Tray Bottom that holds the reagent bottles and vials e One set of reagent bottles 250 ml and 125 ml each and vials 25 ml are supplied with the unit and also available separately page 33 e Reagent Tray top e Clean assist attachment optional allows you to discard any unused reagents into the sink without having to remove the vials separately Instrument with Reagent Drawer open Reagent Tray Assembly Clean assist attachment 250 ml reagent bottles 25 ml reagent vials 125 ml reagent bottles Reagent Tray top Reagent Tray bottom Waste basin Drawer unit Reagent drawer Continued on next page Description of Parts Continued Blot Holder The Blot Holders set of 10 are supplied with the BenchPro 4100 Western Cards The Blot Holder enables easy transfer of the membrane in and out of the card and prevents the membrane from sticking to the card chamber sides
26. h step is completed the reagents are cycled into the waste container or back into the reagent bottle and vial if desired At the end of the protocol the Blot Holder is removed from the card and the membrane is removed from the Blot Holder Perform membrane incubation in substrate as recommended by the substrate manufacturer or follow your usual detection protocol Important features of the BenchPro system are listed below e User friendly BenchPro 4100 Card Processing Station design with a disposable BenchPro Card for easy automated reagent processing for standard laboratory procedures such as immunodetection e Ability to perform simultaneous processing of 1 4 membranes with different reagent sets using the same protocol e Nocross contamination between samples as reagents for each membrane are isolated e Two pre programmed WesternBreeze immunodetection protocols one ECL protocol and the option to customize up to 20 immunodetection protocols e Optional auto drain feature on the waste tray removes the need for frequent emptying of waste reagents e Provides consistent reliable results comparable to manual processing e Built in safety features in the instrument enhance user safety Continued on next page Overview Continued Purpose of the Manual This manual provides the following information e Details and specifications on the BenchPro 4100 Card Processing Station and BenchPro 4100 Western Card e Unpa
27. ical assistance call write fax or email Additional international offices are listed on our web page www invitrogen com Corporate Headquarters Japanese Headquarters European Headquarters 5791 Van Allen Way LOOP X Bldg 6F Inchinnan Business Park Carlsbad CA 92008 USA 3 9 15 Kaigan 3 Fountain Drive Tel 1 760 603 7200 Minato ku Tokyo 108 0022 Paisley PA4 9RF UK Tel Toll Free 1 800 955 6288 Tel 81 3 5730 6509 Tel 44 0 141 814 6100 Fax 1 760 602 6500 Fax 81 3 5730 6519 Tech Fax 44 0 141 814 6117 E mail tech_support invitrogen com E mail jpinfo invitrogen com E mail eurotech invitrogen com 34 Purchaser Notification Limited Use Label License No 5 Invitrogen Technology The purchase of this product conveys to the buyer the non transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer whether the buyer is an academic or for profit entity The buyer cannot sell or otherwise transfer a this product b its components or c materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes The buyer may transfer information or materials made through the use of this product to a scientific collaborator provided that such transfer is not for any Commercial Purpose and that such collaborator agrees in writing a no
28. ils Card Slots Each BenchPro 4100 Card Processing Station unit features 4 card slots that allow the insertion of disposable BenchPro Cards see next page for details on the card into the unit There is no need to use all 4 cards at the same time You can use a single card or from 1 and 4 cards at a time Do not insert anything fingers pipette or liquid into the card slots as it may cause harm to you or damage the unit Digital Display with Function Keys The digital display along with the function keys is used to operate the instrument and choose or customize the processing protocol Continued on next page Description of Parts Continued BenchPro 4100 Western Card The BenchPro 4100 Western Card is a disposable plastic card designed for use with the BenchPro 4100 Card Processing Station see figure below The BenchPro 4100 Western Card aspirates reagents from the reagent bottles and vials into the blot chamber in a sequence determined by the protocol Once the blot chamber is full the solution is recirculated within the chamber for a specified incubation period after which the solution is directed to the waste basin or optionally back into the container from which the reagent was drawn The preprogrammed or custom protocols control the volume of reagents required and incubation times used BenchPro 4100 Western Cards are available from Invitrogen page 33 Always store the BenchPro 4100 Western
29. ith the BenchPro 4100 Card Processing Products Station and are available separately from Invitrogen For more information visit www invitrogen com or contact Technical Support page 33 BenchPro 4100 Western Card 1 box of 10 cards and 10 WP1001 blot holders Novex ECL Chemiluminescent Substrate Reagent Kit WP20005 HRP Chemiluminescent Substrate Reagent A 125 ml HRP Chemiluminescent Substrate Reagent B 125 ml WesternBreeze Blocker Diluent part A and B 2 x 80 ml WB7050 WesternBreeze Wash Solution 16X 2 x 100 ml WB7003 Novex AP Chromogenic Substrate 250 ml WP20001 Novex HRP Chromogenic Substrate 250 ml WP20004 Nitrocellulose membranes 0 2 um pores size LC2000 Nitrocellulose membranes 0 45 um pores size 20 membrane filter paper 1 02001 dwich PVDF membranes 0 2 um pores size coon LC2002 Invitrolon PVDF Filter Paper Sandwiches LC2005 Antibodies A large variety of primary and secondary antibodies are available from Invitrogen For details visit www invitrogen com antibodies 33 Technical Support Web Resources Visit the Invitrogen Web site at www invitrogen com for e Technical resources including manuals vector maps and sequences application notes MSDSs FAQs formulations citations handbooks etc e Complete technical support contact information e Access to the Invitrogen Online Catalog e Additional product information and special offers Contact Us For more information or techn
30. n and wash times are reduced resulting in sensitivities of 55 to 90 of the standard protocol depending on the antigen antibody Choose WesternBreeze 176 min for a standard WesternBreeze immunodetection protocol using standard washing blocking and antibody incubation steps and time Choose ECL protocol for a standard ECL detection To create a custom protocol see page 23 Protocols WestemBreeze 95 Min Note The preprogrammed protocols have a lock icon next to each protocol indicating that these protocols are locked for editing The Protocol Detail View is displayed Verify the protocol steps shown by navigating with the Up down arrow keys Protocol Detail View WesternBreeze 95 Min 1 Block 10 min 2 1 Ab 30 min Return 3 Wash 11 min 4 2 Ab 30 min 5 Wash 11 min Back Edit Half Edit Full Half Full Continued on next page Getting Started Continued Selecting a If changes are required press the Edit Half or Edit Full function key and Preprogrammed proceed to editing the protocol page 23 Protocol Continued Note Selecting Half or Full allows the instrument to calculate reagent volumes required for each step If maximum available reagent volumes are exceeded an error message screen is displayed 4 Select the option to run a protocol for half or full membrane by pressing the Half or Full function key Protocol Detail View WesternBreeze 95 Min 1 Block 10 min 2
31. n of Ensure that transfer buffer contains 10 20 methanol Use blot proteins or protein membranes with appropriate binding capacity weakly bound to membrane Inactive or overly Determine antibody activity by performing a dot blot dilute primary or Increase antibody concentration as necessary secondary antibody High background on the blot Film overexposed or Decrease exposure time or allow signal to further decay became wet during Prevent leakage of solutions by encasing membrane in exposure transparency film and blotting excess substrate from edges before exposure Short blocking time Perform each step for the specified amount of time or washing time High concentration of Determine optimal antibody concentration by performing a primary and or dot blot Decrease antibody concentration as necessary secondary antibody Membrane solutions Use clean glassware and purified water to prepare solutions trays or vials are Rinse the tray thoroughly with purified water Although all contaminated reagent bottles and vials can be reused after washing with mild detergent and rinsing with deionized water we do not recommend reusing the 25 ml vials to prevent any reagent cross contamination Wear clean gloves at all times Use forceps when handling membranes Protein is overloaded Reduce load or dilute concentration of sample Insufficient removal Follow instructions for membrane preparation before of SDS or we
32. ng Processing Time Instrument Dimensions Weight Built in Features 100 240 VAC 3 0 Amps 50 60 Hz Indoor use only Up to 2 000 meters 4 40 C 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C lt 70 dBa Benchtop processor unit with 4 slots 1 to 4 membranes run Variable see each protocol for details 18 6 inches w x 23 9 inches d x 12 7 inches h 30 pounds 13 6 kg Digital display light LED The BenchPro 4100 Card Processing Station including the BenchPro 4100 Western Card is compatible with standard nonhazardous laboratory reagents Do not use organic solvents in the card or with the instrument BenchPro 4100 The BenchPro 4100 Western Cards are used with the BenchPro 4100 Card Western Card Processing Station and are available from Invitrogen page 33 For details on the Specifications BenchPro 4100 Western Cards see page 5 The specifications for the BenchPro 4100 Western Cards are listed below Card Dimensions Card Materials Membrane Size Card Tip Dimensions 18 5 cm x 16 cm Mixed plastics and metal foil Suitable for use with mini blot 8 5 x 8 5 cm 13 2 cm x 6 5 mm The CE mark symbolizes that the product conforms to all applicable European Community provisions for which this marking is required Operation of the BenchPro 4100 Card Processing Station is subject to the conditions described in this manual The protection pro
33. oratory coat and safety glasses during handling the membranes assembling reagents or cleaning the system The BenchPro pre programmed immunodetection protocols are designed to use one primary antibody and one secondary antibody If your detection protocol requires an additional detection reagent required when using a biotin streptavidin reagent pair you need to modify the protocol as well as reagent addition to the reagent bottles For example load blocking solution in the 125 ml reagent bottle instead of 25 ml vial This would allow the use of 25 ml vials for the primary antibody and biotin streptavidin pair Always use the BenchPro 4100 Card Processing Station with BenchPro 4100 Western Cards for immunodetection protocols The BenchPro 4100 Card Processing Station is compatible for use with nitrocellulose or PVDF transfer membranes and a variety of immunodetection reagents Do not use expired reagents Before closing the reagent drawer be sure to remove caps of the reagent bottles and vials Make sure the membrane is of an appropriate size to fit in the Blot Holder to ensure proper insertion and processing of the membrane Always place the membrane in the Blot Holder before placing the holder in the chamber Discard the card and waste contents appropriately after use Do not reuse the card The instrument detects used cards by a color indicator spot at the lower right hand corner of the card which turns red after the card
34. perate the BenchPro 4100 Card Processing Station according to the instructions in this manual Failure to comply with the instructions in this manual may create a potential safety hazard and will void the manufacturer s warranty and void the EN61010 1 safety standard certification Invitrogen is not responsible for any injury or damage caused by use of this instrument when operated for purposes which it is not intended All repairs and service should be performed by Invitrogen In an emergency immediately turn the Power Switch Off and unplug the instrument The symbols used on the BenchPro 4100 Card Processing Station and in the manual are explained below The Caution symbol denotes a risk of safety hazard Refer to accompanying documentation The WEEE Waste Electrical and Electronic Equipment symbol indicates that this product should not be disposed of in unsorted municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of WEEE Visit www invitrogen com weee for collection and recycling options Used on the instrument to indicate an area where a potential shock hazard may exist User manual Product Specifications BenchPro 4100 Environmental Conditions Card Processing Input Power Station Installation site Altitude Operating Temperature Specifications Maximum Relative Humidity Noise Level Specifications Instrument Type Sample Processi
35. ranties including implied warranties of merchantability fitness for a particular purpose and non infringement and no other warranties shall be binding upon Invitrogen In no event shall Invitrogen be liable for any special incidental or consequential damages resulting from the use or malfunction of this Instrument or the system with which it is used even if such damages could be anticipated by Invitrogen To obtain service during the warranty period contact Invitrogen Technical Support for further instruction OUT OF WARRANTY SERVICE Contact Invitrogen Technical Support We will be happy to assist you by phone at no charge Repair service if needed will be billed depending on the parts replaced and labor hours needed to repair your instrument You will be billed for shipment of the instrument to the recommended service facility 2009 Life Technologies Corporation All rights reserved For research use only Not intended for any animal or human therapeutic or diagnostic use invitrogen vy Life technologies Corporate Headquarters 5791 Van Allen Way Carlsbad CA 92008 T 1 760 603 7200 F 1 760 602 6500 E tech_support invitrogen com For country specific contact information visit our web site at www invitrogen com
36. se this product or the technology embedded in it for any use other than for research use please contact Out Licensing Life Technologies 5791 Van Allen Way Carlsbad California 92008 or outlicensing lifetech com Continued on next page 35 Purchaser Notification Continued BenchPro 4100 Card Processing Station Warranty 36 Invitrogen warrants to the original purchaser Purchaser that the BenchPro 4100 Card Processing Station Instrument will be free from defects in materials and workmanship for a period of one 1 year from the date of delivery Invitrogen agrees as its sole responsibility under this limited warranty and upon prompt notice of a defect to repair replace or refund purchase price at its discretion any Instrument discovered to be defective within the warranty period This warranty does not include repair replacement or refund necessitated by accident abuse neglect misuse unauthorized repair or modification of the Instrument In the event that Invitrogen determines that the Instrument is in need of repair and not replacement this Standard Warranty includes replacement parts and labor for the Instrument This Standard Warranty does not include shipment of the Instrument to and from service location or travel cost of service engineer the costs of which shall be borne by the Purchaser This Warranty and the remedies set forth herein are exclusive and in lieu of all other express or implied war
37. sed after washing with mild detergent and rinsing with deionized water we do not recommend reusing the 25 ml vials to prevent any reagent cross contamination For any other repairs and service contact Technical Support page 33 Do not perform any repairs or service on the BenchPro 4100 Card Processing Station to avoid damaging the unit Programming a Custom Protocol Introduction Programming a Custom Protocol Editing Existing Protocol Instructions are provided in this section to use the BenchPro 4100 Card Processing Station with the BenchPro 4100 Western Card for automated processing of the transfer membrane using a custom protocol The protocol setup screen allows you to set a custom protocol using various options listed for protocol steps and incubation time Note You can run up to 4 cards using different reagent sets but using the same protocol You may create a custom protocol by e Editing an existing protocol e Adding deleting steps from an existing protocol e Creating a new protocol using new parameters While you can edit an existing standard protocol you must save the modified protocol with a different name 1 From the Protocols screen select the protocol that you wish to edit by highlighting the protocol using the Up Down arrows and pressing OK 2 The Protocol Detail View screen is displayed Press the Edit Half or Edit Full function key In the Edit mode the instrument tracks the reagent vol
38. ste appropriately if the auto drain feature is not used Transport the waste reagents carefully to sink to avoid any spillage Rinse the Waste Basin with water prior to use Note If you are using the automatic waste drain feature the waste collected in the tray is drained via the plastic drain tubing connected to the unit When using the auto drain feature periodically rinse the waste tray to prevent any residue build up To run another protocol press the Back function key to return to the Protocols screen or press the Run function key to run the same protocol Load reagent bottles and vials with appropriate reagents insert new cards and start the protocol as described above There is no cooling period required for the unit between runs Block Diab z O60 Rinse Back Run If you are not using the device turn OFF the power switch located on the left side of the unit Continued on next page 21 Using the BenchPro 4100 Card Processing Station Continued Cleaning and Maintenance 22 Clean the surface of the BenchPro 4100 Card Processing Station with a damp cloth Do not use harsh detergents or solvents to clean the unit Rinse the reagent tray assembly and waste basin with water after each use If bottles and vials are to be reused clean with mild detergent and rinse thoroughly with ultrapure water Do not autoclave trays or reagent bottles and vials Although all reagent bottles and vials can be reu
39. t the protocol See page 20 for additional details on the Abort and Pause functions Towards the end of the protocol make sure you have the reagents prepared for the detection step as recommended by the manufacturer Note If you are using the automatic waste drain feature the waste collected in the tray is automatically diverted to the sink via the plastic tubing connected to the unit Continued on next page Using the BenchPro 4100 Card Processing Station Continued Running a 10 When the protocol is finished the instrument signals the end of processing Preprogrammed with a beep and flashing green light Tthe digital display shows Protocol Protocol Continued Holding WesternBreeze 95 min 00000 Note At this stage you may keep the card and membrane in the instrument until you are ready to develop the membrane with substrate total card use time should not exceed 19 hours The membrane will not dry out as the last reagent is not drained until you press Complete 11 Press the Complete key to drain reagents from cards and release the card The status light continues to flash green while the reagents are drained and then to a flashing red to indicate card removal WesternBreeze 95 min 12 Proceed to Disassembling the BenchPro 4100 Card Processing Station below Continued on next page 19 Using the BenchPro 4100 Card Processing Station Continued Pausing the Protocol Aborting the Protocol Post
40. t to transfer such materials to any third party and b to use such transferred materials and or information solely for research and not for Commercial Purposes Commercial Purposes means any activity by a party for consideration and may include but is not limited to 1 use of the product or its components in manufacturing 2 use of the product or its components to provide a service information or data 3 use of the product or its components for therapeutic diagnostic or prophylactic purposes or 4 resale of the product or its components whether or not such product or its components are resold for use in research For products that are subject to multiple limited use label licenses the terms of the most restrictive limited use label license shall control Life Technologies Corporation will not assert a claim against the buyer of infringement of patents owned or controlled by Life Technologies Corporation which cover this product based upon the manufacture use or sale of a therapeutic clinical diagnostic vaccine or prophylactic product developed in research by the buyer in which this product or its components was employed provided that neither this product nor any of its components was used in the manufacture of such product If the purchaser is not willing to accept the limitations of this limited use statement Life Technologies is willing to accept return of the product with a full refund For information about purchasing a license to u
41. ues change by whole numbers and cannot be less than 1 e Options in the first column are Block 1 Ab 2 Ab Wash or Rinse Selecting these options allow reagents to be drawn from positions 5 4 3 6 and 1 respectively on the Reagent Tray e Times in the second column refers to the number of times a step is done e By setting the indicator function at the far right to Return the reagent from the selected step is returned to the container it was drawn from This is the default setting for primary antibody for pre programmed protocols e Maximum settings are Custom Protocols 20 Substeps 6 Steps substeps 20 Step substep minutes 999 minutes Step substep times 20 times 6 After making the changes press the OK button to accept changes and allow navigation away from the edited step To save the updated custom protocol press the Save function key Enter the protocol name in the Save Protocol screen using letters in the keyboard with the arrow keys Save Protocol Custom 1 lt j gt esej 1 2 3 4 s 6 7 eo e clojefjrje ufijs k t m Njolelajris tjulviw x yiz Clear Ll 9 Navigate within the name using Right Left arrow keys Press the OK function key to select the correct letter when displayed 10 Repeat Step 9 for each letter in the new name To clear the entered letters press the Clear function key 11 Press the Save function key to save the edited protocol under a new name The new protocol no
42. umes required as steps are programmed The of maximum available volume is displayed as a bar at the bottom of the screen When volumes exceed available volumes in reagent bottles and vials an error message is displayed Note The Edit Half and Edit Full functions are used for calculating reagent volumes only the actual quantity of reagents used is determined by the selecting Half or Full from the Run screen Protocol Detail View WesternBreeze 95 Min 1 Block 30 min 2 Rinse 10 min 3 1 Ab 60 min 4 Wash 20 min 5 2 Ab 30 min Back Edit Half Edit Full Full 00000 Continued on next page 25 Programming a Custom Protocol Continued Editing Existing 3 Highlight the step to be edited by using the Up Down arrow keys Protocol Continued Protocol Edit Custom 1 1 Block 1 minute 1 time 1 minute 1 Ab 1 minute Return 4 Highlight the function to be edited by using the Right Left arrow keys At the far right is a column to indicate reagent Return If this indicator is set to return the reagent is returned to the container it was drawn from after use Protocol Edit Custom 1 1 Block 1 minute 1 time 1 minute 1 time 1 minute Back Insert Delete Save Run Continued on next page 24 Programming a Custom Protocol Continued Editing Existing 5 Change the value of the function using the Up Down arrow keys The Protocol continued following rules apply e Numeric val
43. ut of the compartment and inspect the fuse If the fuse is burned or there is a break in the fuse element replace the 250 V 1 25 A fuse with the identical type fuse 4 Place the fuse holder back into the compartment and snap the cover closed 31 Repackaging the Instrument Repackaging and Storage Instructions 32 If you need to send the instrument to Invitrogen for repair or warranty issues or you wish to transport the instrument to another location repackage the unit as follows 1 Turn off the main power switch at the left side of the instrument and detach the power cord from the rear of the instrument 2 Disconnect the drain tubing connected to the instrument via the connector at the back of the unit 3 Remove the bottles and vials from the drawer unit and secure the drawer unit with a tape 4 Place the instrument in the original box including the original packing foam If you have not saved the original packaging material the appropriate packaging material for the instrument is available by contacting Technical Support page 33 5 Tape the box securely and place appropriate shipping labels for shipping the instrument to Invitrogen Always transport the box with the instrument in the upright position If the instrument is not to be used for extended periods of time store the repackaged instrument in an upright position at 4 C to 40 C Accessory Products Additional The following products are for use w
44. vided by the instrument may be impaired if the instrument is used in a manner not specified by Invitrogen Canadian standards A CSA mark with the indicators C and US means that the product is certified for both the U S and Canadian markets to the applicable U S and vii Unpacking the BenchPro 4100 Card Processing Station Unpacking Instructions vill Follow the instructions below to unpack the BenchPro 4100 Card Processing Station The weight of the instrument is 30 pounds 13 6 kg 1 Cut plastic tapes and remove the outer box Save the outer box and other packaging material in case you need to transport or ship the unit Remove the plastic bag from the top containing the manual and QRC and then remove plastic bags containing the power cords and plastic drain tubing from spaces around the instrument Remove the BenchPro 4100 Card Processing Station from the box and place on a flat level surface Remove any tape holding the reagent drawer and tray parts The plastic bottles and vials are placed in position in the tray during shipping Set up the BenchPro 4100 Card Processing Station as described on page 8 BenchPro 4100 Card Processing Station Front View The front view showing various parts of the BenchPro 4100 Card Processing Station is shown below 4 Card slots Digital display and Function keys Rear and Side The rear and side view showing various parts of the BenchPro 4100 Card View
45. w appears on the Protocols screen 12 Proceed to assembling the unit with reagents and card page 12 to perform the custom blotting protocol Continued on next page 25 Programming a Custom Protocol Continued Inserting Adding 1 or Deleting a Step or Sub step to 2 Existing Protocol 26 From the Protocols screen select the protocol that you wish to edit by highlighting the protocol using the Up Down arrows and pressing OK Once the protocol steps are displayed press the Edit Half or Edit Full function key Select and highlight the step to be edited by using the Up Down arrow keys Press the appropriate function key based on the operation that you wish to perform e Select Insert function key to place a new line below a highlighted line After selecting the Insert press the Step function key to add a new step or Sub step function key to add a sub step within a step For example If a 1 x 5 minute wash is followed by 1 x 15 minute wash the 5 minute and 15 minute operations are sub steps of the 20 minute wash step Edit the inserted lines as needed as described in Editing Existing Protocols page 23 e Select Delete function key to delete a highlighted step or sub step Protocol Edit Custom 1 1 Block 1 minute 1 time 1 minute 1 Ab 1 minute Return Back Insert Delete Save Run After making the changes press the OK button to accept the changes and allow navigation away from the edited step
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