LABScan3D and xPONENT 4.2 Quick Reference Guide
Contents
1. USP Plate Layout New Batch Cancel 18 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide LABScan3D Unit Shutdown 1 On the Maintenance tab select the Auto Maint sub tab 2 Select the System Shutdown bubble 3 Eject e the tray and fill the correct reservoirs with 10 bleach solution and DI water Ban Ou 4 a A are gt a Home Samples ty Batches Results S Protocols JD Maintenance 4 Admin Luminex Automated Maintenance System Shutdown 4 Auto Maint Instructions Select the Automated Maintenance Option or Maintenance Command Then select the appropnate kit if applicabile i Automated Maintenance Options O A Cr Calibration Performance Fluidics Venficabon Venficabon Prep Reagent Status Reagents Sanitize RB2 10 Bleach Pending Gniiyaioa K2 Performance Verification Kit B35628 v B33865 Wash RAI DIH20 Pending Legend Wash RA1 DI H20 Pending Alcohol Flush Vortex each reagent vial for 30 seconds Place 5 drops of each reagent into the ated well Fil the dasgnated reservoirs Sanitiz soap ns 70 isopropanol koa 20 household bleach Soak RA1 DI H20 Pending G Saniize le Boarder w Wash San 0 Drain A 1 2 1 z 3 Q FJDCAL1 i c F3DeCAL a c2 F3DCAL2 FJDCAL3 z F3DVER1 FIDEVERT v FIDVER2 4 Retract 2 tray and select the Run aB button at the bottom right corner of the screen 5 Once the System Shutdown is complete2. B Act 4 Batch Name New Batch Date 1 30 2012 7 35 Ph LABScan3D and xPONENT 4 2 Quick Reference Guide 15 One Lambda Inc A Thermo Fisher Scientific Brand 6 Select all the wells to be read by clicking and dragging over them with the cursor Note If creating batches for use in a multi batch DO NOT move the starting well position 8 Next either click Import List to import a Sample ID Import list to the batch or edit the Sample ID of each well by double clicking in the ID field next to the corresponding well position and give each ID a sample name 7 Then select to assign the wells as Unknown See Figure below Note In order to edit Patient IDs Sample IDs after a batch has completed see Appendix G Edit Patient ID Sample ID Notes regarding the Sample ID Import List Note A Sample ID Import list must be a txt file created in notepad or equivalent with one Sample ID per line Note The 2 line heading that was required in Luminex I52 3 Sample ID Import list is no longer required however if present the heading does not affect the Sample IDs imported to the batch LX100IS Patient List Accession Dilution Factor Note The number of samples in the Sample ID Import list must match the number of Unknowns selected If the Sample ID Import list is longer than the number of Unknowns selected only the Sample IDs equaling the number of Unknowns on the plate will be imported If the Sample ID Import
3. LABScan3D and xPONENT 4 2 Quick Reference Guide Software Version Luminex xPONENT 4 2 Revision May 2015 This is only to be used as a guide Verify this reference against the most current Luminex FLEXMAP 3D Hardware User Manual IVD and Luminex xPONENT 4 2 Software User Manual IVD If questions still exist contact One Lambda Inc Technical Support MS ONE LAMBDA ThermoFisher A Thermo Fisher Scientific Brand SCIENTIFIC Table of Contents Powering on the LABScan3D Unit ennrnnnnnrnnnnnnnnrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennn 4 User Computer Screen Saver and Power Saving SettingS c cccceeeeeeeeeeeees 4 Un hiding Hidden Files and Folders cccccccsccceeeceeecececeeeceeecueesaeeeeeesaeenaes 8 LAB SCANnGD CSV ODTONS SCUIAG S vacua sce datececciezedsecluneudiapcesdieentebocdessecensedaieeniuends 9 Adjusting the Probe Height fora LABType and LABScreen Plate 10 Sy SET ANZ 10 EEE E N ESAE 11 ee ej AE eg EE EE 12 DFTA OM 34 01 1016 0 EE SN rei deacneneananes 15 Create gt eg EE 15 FSP hr 17 PENN Ben 17 LABScan3D Unit SMUTAOWN is ncesencauntvarsinznnidaantentedtendantwansuis oitihebianinivaeieasdmastionetentas 19 OC EDA een 19 DANAN IG see este carer attic eee sen sake E AE AEE 19 Appendices Appendix A Automatic Sample Probe Height Adjustment rrrarerrarernnernnre 20 Appendix B Downloading Calibration Kit or Performance Verification Kit Information for I
4. or scroll down to 0 minutes Select the power plan that you want to customize and B then choose settings that reflect how you want your computer to manage power E Wweless Adapter Settings s Sleep a USB settings Power buttons and hd PO Fess a Select the power plan that you want to customize and then choose settings that reflect how you want your computer to manage power Select the power plan that you want to customize and then choose settings that reflect how you went your computer to manage power Balanced Actrve 7 Seep D USB settings Power buttons and bd 6 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide 6 Again select the Control Panel from the Windows Start menu Select View by Large icons then click on the Display icon T Ppa may TESTE Search Control f P G 53 gt Control Panel All Cortsel Pandi kema Adjust your computer s settings WY Action Center OM Color Management Eg ost Touchpad BH Kase of Access Center a Homes e Internet Options F Administrative Tools Bg AutoPlay Tu Configuration Manager 7 Credential Manager GH Deskop Gadgets BD device manager Flash Player 32 bit E Folder Options E icloud Lill Java 32 bit byl IDT Audio Control Panel em Keyboard be Backup and Restore 5 Date and Time Devices and Printers Fonts op Indexing Optio
5. see Appendix B Downloading Calibration Kit or Performance Verification Kit Information for Import into XPONENT Software b Vortex each performance verification reagent bottle for at least 30 seconds c Eject e the plate and insert one empty plastic strip well into lane two in the strip well holder d According to the image in the display window add 5 drops each of verification reagent beads to the designated wells inverting the vials completely when adding the drops e Before retracting the plate be sure there is sufficient DI water in the water reservolr f Retract plate and select Run gt Luminex Ga Home Samples f Batches Automated Maintenance Performance Verification Auto Maint Instructions Select the Automated Maintenance Option or Maintenance Command Then select the appropnate kit if appicable O Automated Maintenance Opsag Lot Management Cmds amp Routines r a eshe re Reagents Calibrabon Kz Performance Venfication Kit B35628 833865 None Probe amp Heater Legend 14 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide Import Protocol 1 In the LABScan3D window select the Protocols tab 2 Click the Import button at the bottom See Figure below P EE A Home Samples ty Batches Pr Results E Protocols amp Maintenance Admin Protocols Protocols Instructions Create or import a New Protocol or
6. 05 PM 071514 DRXR 01 JC RSSOX2B1R_001 002 7116 2014 10 55 PM 071514 DRCWD 01 RSSOW2B1R 001 002 7 16 2014 10 48 PM RSSO2P LS3D 005 001 7115 2014 7 12 PM RSS02Q 009 002 7 15 2014 7 08 PM RSSOH1C_005 002 7115 2014 7 05 PM RSSOH1C 005 002 7115 2014 7 01 PM RSSOW2B1R_001 002 7115 2014 6 52 PM RSSOX2B1R_001 002 7 15 2014 6 45 PM RSSOH2B1 008 002 7 15 2014 6 38 PM RSS02B1 LS3D 017 002 7115 2014 6 35 PM RSSOW2B1R_001 002 7 15 2014 6 31 PM Save A Exp Results 7 LABScan3D and xPONENT 4 2 Quick Reference Guide 31 One Lambda Inc A Thermo Fisher Scientific Brand Appendix H Maintaining Fluids 1 Periodically monitor sheath fluid levels Replace the sheath fluid container as needed as well as the waste fluid container Note If the LABScan3D instrument operates with an empty sheath fluid container the lack of sheath fluid may interrupt a sample acquision and prevent further data from being collected CAUTION Use only xMAP Sheath Fluid or another Luminex approved sheath fluid Use of any other sheath fluid constitutes improper use and can void the warranty provided by Luminex and or its authorized partner 2 Optimally the waste line should be routed to a laboratory drain or waste container a If a waste container is used monitor waste fluid levels periodically The volume of the waste container should be at least as large as the volume of the sheath container b Do not allow the waste fluid container to overflow Empty the wa
7. If the Calibration Kit or Performance Verification Kit CDs are misplaced the same information is also available on the Luminex website http www luminexcorp com a At the top go to the Resources tab and on the pull down menu under Target Values click on FLEXMAP 3D gt b Locate and save as the corresponding calibration or performance verification kit to a known location on the computer On the xPONENT software select the Maintenance tab and click on the Lot Management sub tab On the bottom right hand corner of the display window click Import Kit r A browser window will appear browse to the location of the CD drive on the computer or the known location of the information downloaded from the Luminex website Open the IxI file for the corresponding kit if the kit imports properly a prompt will appear verifying the kits installation Once the kit is imported select the kit from the pull down menu Calibration Kit or Performance Verification Kit to activate the lot Luminex er Gir Gan m fa Home ar Samples f Batches Results S Protocols k Maintenance ra Admin f Lot Management Auto Maint instructions Import export delete or create new Calbration and verificabon tot or kit formation Activate reagents by selecting the KR or lot number 6 Active Reagents Lot Details Lot Management Calibration Kit Performance Venfication Kit Lot Type F3DCAL 1 Expiration Da
8. Reference Guide Appendix J Schedule 1 Select the Admin tab gt Schedule a The schedule tab displays 6 Month and 12 Month PM due dates Luminex User f Batches S Protocols fa Home ar Samples fi Results a Gao Q nm XPONENT A Ad mn f Maintenance Ep Scheduled Maintenance System Setup instructions Enable or change Recurrence of scheduled Maintenance remenders Enable or Disable the Laser Warm Up schedule Reminders Group Setup Subject Reminder Start Alerttme Recurrence Notes 6 Month Thesis a courtesy reminder that your six month PMis 5 9 2012 10 00AM Semi Annu If your system is covered Scheduled now due In order to help keep your system running lunder a Luminex Gold or PM is Due Optimally Luminex recommends that the following Pabrmum service User Setup items be replaced at this time contract please disregard this notice APM semice call 1 HEPA air fitter wil automatically be 7 2 Syringe Teflon seals generated at Luminex and Batch Options 3 Aar intake filters Aluminum Clean you wil be contacted by one Filter front door right of our Feld Service Filter front door left Engineers to schedule Filter front XY date for thes service Alert Options 12 Month This is a courtesy reminder that your twelve month 5 9 2012 1000AM Yearly If your system rs covered Scheduled PM is now due In order to help keep your system lunder a Luminex service PM is
9. System Schedule Overview Archive Events E See eer 6 9 2014 Tm 08 00 Y AM Y TATQO4ZE DW 2A Remn er PAADID SOA nn Daily r TRUVEO WA Reminder 1723201450031 Reminder Reminders Only TOSTI 8900 33 AM Reminder J Yes 727014 800334 Reminder To Be Archived TRT2014 80033 Reminder 72520148 00 334 Rermmnder Note If the frst two Checkboxes are not checked al batches wil be archived Tames esa A r iniy archive batches older than 14 Days 72201410 304 Reminder Tea rrunder Always keep the last 20 Batches NENNE m m logs older than 30 Days Archive Folder C Wsers Public WDocuments Archive Browse System Current fies in xPONENT Batches 167 Protocots 28 Kits 0 Current Local Disk Space ie 1 Used 6 Used Le LABScan3D and xPONENT 4 2 Quick Reference Guide Lots 0 Current Database Space 33 a1Cfeve 18 due type Automate Oo Select Manual Archive to individually archive files Backup the entre system by selecting Backup System and restore rbeve is due type Automatic archive type Batches Ative h due type Aatomatc archive type Balchew archive is due type Aatomatc archive type Datches archive it due type Aa omate archive type Gatcher archive is Que type Automat archive type Batches acheve type Batches arcteve is due type Automate archive type Batches archive is due type Automatic archive type Batches archeve ib due type Automat archive type Batcher archive is due type Aa om
10. exit the software and turn off the LABScan3D unit by flipping the switch located on the bottom right hand side of the unit Locate Data 1 Once a batch is completed locate the CSV output file and rcsv Run Files for HD or XR kits and transfer them to a known location for data analysis a CSV output Files and Run files are located at the location specified on Admin gt CSV Options See LABScan 3D CSV Options Settings page 9 Data Analysis 1 Refer to the HLA Fusion User Manual LABScan3D and xPONENT 4 2 Quick Reference Guide 19 One Lambda Inc A Thermo Fisher Scientific Brand Appendices Appendix A Automatic Sample Probe Height Adjustment Note Each Protocol is created with a plate type built into the protocol based on the assay type A plate type must be saved for both the LABType Assay and the LABScreen Assay Protocols Note The probe height should be re adjusted and saved for all plate types each time the probe is removed and replaced in the unit Note The probe height should be adjusted before the first use of a new reading tray type brand lot Example If the type of plate being used to run either the LABType or LABScreen Assay is changed the LABType Plate and or the LABScreen Plate must be re adjusted and saved based on the probe height for the new plate Place the new plate in the unit select the Plate from the Plate Name pull down menu and click Auto Adjust Height P The new height will be automaticall
11. plate types each time the probe is removed and replaced in the unit Note The probe height should be adjusted before the first use of a new reading tray type brand lot Example If the type of plate being used to run either the LABType or LABScreen Assay is changed the LABType Plate and or the LABScreen Plate must be re adjusted and saved based on the probe height for the new plate Place the new plate in the unit select the Plate from the Plate Name pull down menu and click Auto Adjust Height The new height will be automatically updated to the selected plate 1 Refer to Appendix A Automatic Sample Probe Height Adjustment for instructions on adjusting the probe height 2 When naming the plate name the plate exactly LABType Plate Place the tray that will be used to read LABType assays onto the XY platform of the LABScan3D unit auto Pus 5 Create a new plate when naming the plate name the plate exactly LABScreen Plate 6 Place the tray that will be used to read LABScreen assays onto the XY platform of the LABScan3D unit Note If using a filter bottom plate place a large 5 08mm alignment disk into the selected well for other hard bottom plates no disk or metal ball is required 10 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide Auto Adjust Height 7 Click Auto Adjust Height 4 The height will automatically save to the Plate Name Ka Home vi Sam
12. select an existing Protocol from the list below to export edit view or delete Stds amp Ctris Create New Protocol Click to create a new Protocol Installed Protocols Name i Manufacturer Date LS1A04008_LS3D One Lambda Inc 5 712014 4 49 PM LS1A04008_LS3D_FILTERPLA One Lambda Inc 6 13 2014 4 23 PM dan KE New Std Ctrl Plate Layout Delete Export 3 Browse to the location of the saved protocol information 4 A prompt will open saying that the protocol was successfully imported 5 The imported protocol should be displayed under Installed Protocols on the Protocols tab Create a Batch 1 Select the Batches tab ea at the top of the display window the following options shown below will be displayed in the window Create a New Batch from isti Click to create a new batch from an existing protocol an existing Protocol 9 protot Create a New Batch from a new Protocol Chick to create new batch vathout using protocol Create a New Multi Batch Chick to create new multi batch from pending batches Click Create a New Batch from an existing Protocol fig Enter a Batch Name for the samples to be run See Figure below Select the protocol to be used from the Select a Protocol list Click Next in the bottom right of screen _ m a a a a S Protocols Maintenance g Admin mae Step 1 Select Protocol for New Batch 7 Instructions Name thes batch ar elec
13. the 1st assay is run for EACH user login 1 From the Windows Start menu select Control Panel HLA Fusion 30 P Microsoft PowerPoint 2010 xe Microsoft Excel 2010 wi Microsoft Word 2010 HLA Fusion 3 0 Research HLA SBT uTYPE 6D b Database Utility 3 0 2 In the Control Panel window if not already selected select View by Large icons then click on the Power Options icon GU 38 gt Control Panel All Cortrel Panel kema Adjust your computer s settings W Action Center GME Color Management Eg elt Touchpad GB Ease of Access Center some B Internet Options DP mouse GB Phone and Modem 9 Region and Language V R system Windows Defender S rm Administrative Tools a Configuration Manager Desktop Gadgets Flash Player 32 bit ae iCloud ah Java 32 bit Sm Network and Sharing Center RemoteApp and Desktop 2 Connections Taskbar and Start Meru a Windows Firewall 4 One Lambda Inc A Thermo Fisher Scientific Brand iat faa G 4 Search Contr P Large kom Bg AutoPlay Ma Backup and Restore S sitockdf Drive Smaticons 7 Credential Manager gy Date and Time e Default Programs BD Device manager ig Devices and Printers BD visptay JE Folder options roms 1 Getting Started by IDT Audio Control Panel de Indexing Options D Pete Graphics and lt gt Keyboard EN nd BD Mail 32 bit Ei Performance Information era a Personalization ceed Notificati
14. xPONENT 4 2 Quick Reference Guide Note Verify that the lot numbers of the calibration and performance verification reagent bottles in use match up to the corresponding kit lot numbers Lot information is located under Maintenance gt Lot Management If a calibration or performance verification kit needs to be imported to the LABScan3D software see Appendix B Downloading Calibration Kit or Performance Verification Kit Information for Import into XPONENT Software Note For information regarding the purpose of each Calibration and Verification reagent see Appendix C Calibration and Verification Bead Information b Vortex each calibration and performance verification reagent bottle for at least 30 seconds c Eject the plate and insert two empty plastic strip wells into lanes 1 and 2 in the strip well holder d According to the image in the display window add 5 drops each of calibration and verification reagent beads to the designated wells inverting the vials completely when adding the drops e Before retracting the plate be sure there is sufficient DI water in the water reservoir f Retract J plate and select Run gt e Luminex e Ka Home vi Samples fi Batches Results a f Automated Maintenance Calibration Verification Auto Maint Instructions Select the Automated Maintenance Option or Maintenance Command Then select the appropriate kit if appbcable intenance Options OIL z Cal
15. 12 219 5114 For Luminex License Code Information Go to Admin gt Licensing For Luminex Serial Number Go to Maintenance gt System Info onelambda com 2015 Thermo Fisher Scientific Inc All rights reserved Luminex XPONENT and FLEXMAP 3D are trademarks of Luminex Cor poration All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries Specifications terms and pricing DN O N E LAM B DA are subject to change Not all products are available in all countries Please consult your local sales representative for details A Thermo Fisher Scientific Brand One Lambda Inc A Thermo Fisher Scientific Brand 21001 Kittridge Street C Canoga Park CA 91303 MARKETING MAT QR LABScan3D 00
16. 15 2014 6 31 PM 2 99 Save Prtcl Plate Layout Approve Exp Results 3 Select the Edit Patients button at the bottom Luminex i a Protocols Maintenance P 072514 FMR TURK A 014 JC created 7 25 2014 7 15 29 PM Instructions View the results for this batch Select the Information icon for more information on results options Save Image Formula Vakdate Invahbdate 4 An Edit Patient List window will open select the IDs to change and edit the ID names Note An entire new Import List may be imported by clicking Import List 30 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide 5 Once all IDs have been edited click Save Edit Patient List Edit the Patient IDs manually or import a patient list To manually make changes click a row to edit then click on the patient ID entry 6 Re select the batch and then click the Exp Results button 7 Results will be exported to location indicated in LABScan3D CSV Options Settings see page 9 Luminex Home ar Samples poz Saved Batches instructions Select a batch to open and wew its details export approve or replay Current Batch Filter Filter is OFF Saved Batches Completed Batches LIS Results 071514_DRHD_08_ RSSOH2B1 008 002 Reports 071514 DR 017 JC RSSO2B1_LS3D_017 002 7 16 2014 11
17. A 45 D D System Status Move Probe Down Schedule Support Utility Current Curreet height is the Current Current Height fiset value tom Height Hesght where the probe was adjusted to the bottom 0 49 0 49 of Me Yay the disk or Ya msn t gt 4 gt neghi stuntmenn Save negt sdustrects neghi stuntmenn are aut saved tor Plate se at saved for av a gt saved tor ra aes ma res ra yo 20 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide 4 If using a filter bottom plate place a large 5 08mm alignment disk into the selected well for other hard bottom plates no disk or metal ball is required Note Verify that the plate is not warped Warped plates may lead to incorrect probe height adjustment Click Eject at the bottom of the screen Place a new empty plate onto the plate holder with well A1 in the top left hand corner Insert a new empty plastic strip well into the selected lane and place the reservoir into the reservoir holder NSM Note Verify that all selected wells and reservoirs are EMPTY and DRY 8 Click Retract a uto Adjust LABScan3D and xPONENT 4 2 Quick Reference Guide 21 One Lambda Inc A Thermo Fisher Scientific Brand Appendix B Downloading Calibration Kit or Performance Verification Kit Information for Import into xPONENT Software Insert the Calibration Kit or Performance Verification Kit CD into the CD drive on the computer Note
18. Due running optimally Lumsnex recommends that the contract please disregard following items be replaced at thes time this notice APM serve call CSV Options will automatically be 1 HEPA air filter generated at Luminex and 7 2 Syringe Teflon seals you wil be contacted by one 3 Sheath filter wiquick disconnect of our Freki Service Archive 4 Outside Sample tube Engineers to schedule a 5 Valve to Purnp tubings date for thes service 6 Peek tubing Se falve rotor 250K cycles Licensing 7 Sample Valve rotor F OK cycles Laser Warm Up Schedule Schedule amp 7 Enable Schedule Warm Up 9 08 30 AM Report Options b Laser warm up may also be schedule from this tab Note Laser warm up is also available from every screen by clicking the Warm Up button Note It takes approximately 30 minutes for the lasers to warm up before being able to Calibrate or Verify the unit 37 One Lambda Inc A Thermo Fisher Scientific Brand 38 Appendix K Support Utility If Luminex technical support requests the Support Utility Output File the Support Utility maybe used to generate the Support Utility Output File 1 Click the Maintenance tab gt Support Utility Note In order to include Batch info make sure to tick the Include Batch Info check box and select the batch to include below 2 Click the Support button and the Support Utility will open in a new window Auto Maint i
19. For Folder window will open f Browse to a location to backup the system to Default C Users Public Documents Archive and click OK Archive Utility Instructions Setup a schedule for Automatic Reminders or Archives Select Manual Archive to individually archive files Backur the system from previous backup by selecting Restore System Schedule Overview Archive Events First Occurrence Date Log Type ar5 2014 7 46 17 AM Reminder 06 4100 y Am v Frequency Daily Browse For Folder Reminder Only 4 Yes To Be Archived a Public Mote If the first two checkboxes are not checked all batches will Public Documents W Only archive batches older than 14 gt Days Archive Always keep the last 20 e Batches dk Backup Archive all system logs older than 30 E Da LocalArchive Reports Public Downloads gt L Public Music b Public Pictures D Public Recorded TV uhl Archive Folder C Users Public Documents Archive LABScan3D and xPONENT 4 2 Quick Reference Guide 35 One Lambda Inc A Thermo Fisher Scientific Brand g The following prompt will appear in the bottom right corner of the screen h Once completed the following prompt will appear in the bottom right corner of the screen X the prompt 36 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide LABScan3D and xPONENT 4 2 Quick
20. Routine Name pull down menu f Fill Reservoir RA1 with DI water RA2 with 0 1N NaOH RB1 with 70 Alcohol and RB2 with 10 20 Bleach Also place an empty stripwell into S1 g Click Run Ka Home vi Samples fi Batches M Results S Protocols Maintenance Commands and Routines Instructions Run single or multiple commands or run a routine Create a routine for the selected commands by cbcking Save As Rowane Name Stringent Clean Plate Name Current 96 well plate 2 3 4 Ly amp s 10 te FJDVER ev X FJDEVER Alcobvol Flush Warmup System Info A System Status LABScan3D and xPONENT 4 2 Quick Reference Guide 25 One Lambda Inc A Thermo Fisher Scientific Brand 4 Infrequent Use of Unit If a unit has not been used for over a week a Revive After Storage Luminex routine should be run on the unit before use a Select the Maintenance Tab gt Cmds amp Routines b In the Routine Name pull down select Revive After Storage Luminex notice that Command Sequence populates with the routine c Fill the appropriate reservoirs with required buffers d Click Run P 4 sr 2 Gi M Home Samples 4 Batches Results Protocols HD Maintenance Admin Ka uu P f 3 Ji Maintenance Commands and Routines Auto Maint Instructions Rur i i Lot Management Cmds amp Routines TS Probe amp Heater i 5 m I System Info g
21. a plan and customize it by changing its power settings Tell me more about power Choose what the power plans buttons do Preferred plans Create a power plan 2 Choose when to turn off the Change plan settings display Automatically balances performance with energy consumption on capable hardware Change when the computer sleeps Power saver Change plan settings Saves energy by reducing your computer s performance where possible Show additional plans v 4 In the Change settings for the plan Balanced change the Turn off the display pull down to Never below and click Save changes Clicking Save Changes will take the user back out to the Power Options page re select Change plan settings same as above then click on Change advance power settings below Fi dl o yr 2 All Control Panel Items Power Options Edit Plan Settings Change settings for the plan Balanced Choose the sleep and display settings that you want your computer to use Change advanced power settings Restore default settings for this plan LABScan3D and xPONENT 4 2 Quick Reference Guide 5 One Lambda Inc A Thermo Fisher Scientific Brand 5 On the Advanced settings window make sure that the Hard Disk gt Turn off hard disk after is set to Never the Sleep gt Hibernate after is set to Never the Display gt Turn off display after is set to Never and click Apply OK and Exit Note Manually type in Never
22. and xPONENT 4 2 Quick Reference Guide 11 One Lambda Inc A Thermo Fisher Scientific Brand 2 As indicated on the screen fill the corresponding reservoirs with 70 alcohol solution and DI water a 1 3 Select the Run gt button Other routines may be found under the Routine Name pull down menu Calibration Verification Note In order to maintain the LABScan3D system 6 month and 12 month PMs should be performed by Luminex see Appendix J Schedule to determine PM due dates Note If Calibration or Performance Verification fails see Appendix D Basic Instrument Troubleshooting 1 The LABScan3D unit must Calibrated every 7 days and the Performance Verification kit must be run every day the unit is used If it has been more than seven days since the previous calibration see Step 2 if the unit has been calibrated in the past seven days see Step 3 Note The Calibration and Performance Verification status of the unit may be checked on the Maintenance tab by selecting System Info sub tab on the left hand side Calibration Verification 2 On the Maintenance tab select the Auto Maint sub tab then select the bubble for Calibration Verification Note Calibration must be followed by Performance Verification a Select the correct lots of the Calibration Kit and Performance Verification Kit in each of the corresponding pull down menus See Figure below 12 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and
23. atc archive type Batches archive ts due type Aa omatec archive type Vatches LIS Records 0 Current Local Archive Space 6 Used One Lambda Inc A Thermo Fisher Scientific Brand e Select the Batches to be archived click the gt to add the selected batches and click the Archive button click the gt gt to add all of the batches r Manual Archive Q To Be Archived ae Pre nae rc versar Dote Site Une 12 12 13 PP4559 CWD B L1 11T 8K 22K MH PP4559 S 01 03 14 PP4581 AXR LT11T GM Testi PP4559 80 Logs Test2 PP4559 80 AXR LOTO1 051414 RSSOXLAR BXR LOTO1 051414 RSSOX1BR Protocols DRX_LOTO1_051414 RSSOX2B1 test LSM12019 gt gt New Batch 1 LSM12019 Kits Hans 05 14 14 PP4559 80 01 03 14 PP4581 AXR LT11T GM 4 2 PP4581 Te lt AXR LOTO1 051514 RSSOXLAR Lots BXR_LOTO1_051514 RSSOX1BR lt lt DRX_LOTO1_051514 RSSOX2B1 05 15 14 A_CWD_BRAZIL RSSOWLAI LIS Records 05 15 14 B CWD BRAZIL RSSOW1BI 05 15 14 DR CWD_BRAZIL RSSOW2B 05 15 14 A XR BRAZIL RSSOXLAR 05 15 14 8 XR BRAZIL RSSOX1BR 05 15 14 DR_XR_BRAZIL RSSOX2B1 m ni 2 The Archive Utility also allows the user to backup the entire xPONENT database a Select the Windows Start menu b Select the following file path All Programs gt Luminex gt Common gt XPONENT Archive Il Luminex lt gt Luminex Support xPONENT Maintenance 4 Back p j Pra Lorre ET NE bilar EGrch programs and fue c The Archive Utility will launch 34 On
24. batch to be read and repeat until all batches are added See Figure below 5 Click Eject at the bottom of the screen and load the plate to be read Note Check the plate orientation and verify that well A1 is at the top left hand corner of the plate holder 6 Click J Retract 7 When ready to begin playing the batch click Run 2 at the bottom right hand corner of the screen Note Multi Batches may be saved and run at a later time In order to save the Multi Batch name the Multi Batch under Multi Batch Name and click the Save button The Multi Batch will appear on the Batches tab under Pending Batches 8 After the tray has completed running if any samples need to be re run see Appendix E Re reading Individual Sample Wells or if a batch needs to be replayed see Appendix F Replaying a Batch 9 Once completed click Eject e and remove the plate 10 Click Retract oy User Administrator EI sog om XPONENT D Cz Batches Protocols amp Maintenance g Admin No E LE Create New Multi Batch Batches Instructions Add pending batch to create and run a multi batch Select a batch from the list then select Move to change a batch start location or select Remove to remove a i batch a Multi Batch Name name multi batch here Multi Batch Batc Name Start ed ae 1 NewBaht 1At New Multi Batch E 8 9 10 11 12 1 2 3 4 5 6 00000 00060 9000 0000 9000 0000 0000 0000
25. can3D and xPONENT 4 2 Quick Reference Guide Settings Preview ne display logon screen Power management Conserve energy or maximize performance by adjusting display brightness and other power settings Change power settings Ca me One Lambda Inc A Thermo Fisher Scientific Brand Un hiding Hidden Files and Folders Must be set for EACH user login 1 Again select the Control Panel from the Windows Start menu Select View by Large icons then click on the Folder Options icon Adjust your computer s settings View by Large icons En Lugeikom WW Action Center F administrative Tools EJ Atopy Ma backup and Restore S BitLocker Drive Smetlicom GW Color Management By Configuration Manager E Credential Manager ae Date and Time vetsut Programs E oe Touchpad GY Desktop Gadgets BD Device manager ia Devices and Printers E oispisy Q Esse of Access Center Flash Player 32 bit roms Getting stared _Homesroup ES ictous FRB tor Audio control Panel Ja Indexing Options me P internet Options a Java 32 bi gt Keyboard i emne ED mail 32 bit 2 In the Folder Options window select the View tab select the Show hidden files folders and drives radio button click Apply OK and Exit You can apply the view such as Detafs or icons that yOu are using for this folder to all folders of this type Apply to Folders Advanced settings d Files and Folders F Aways show icons
26. e Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide d Select the System Backup button from the bottom of the Archive Utility window Archive Utility instructions Setup schedule tor Automatic Reminders or Archives the system from previous backup by selecting Restore System Schedule Overview First Occurrence Frequency Reminder Only To Be Archived Note If he frst two checkbowes are not checked af batches wil be archived a Only archive batches v Days older han 14 Always keep the last 20 Batches Agchive all system logs older than 30 Days Archive Folder C Wsers PubliciDocumants Archive Edit Current files in xPONENT Batches 100 Current Local Disk Space ae ar By Archive Events Date 65 2014 112 12 Pv 652014 1 12 12 Pw 662014 3 00 27 AM 62014 800 29 AM 1204 8 OG 31 Ad VID SWRA GHOD 800 MA wu SOMA 16204 60037 Ww 620204 800394 ERDO 800414 824204 800018 626204 5 09 0 4 8282014 8 00 04 4 bodd 7 2422 PI OZADI 7 37 16 P1 5902014 800064 12014 8 00 07 AM 12014 8 90 07 AM 8907014 8 OG DG 4 B2N2014 73716 Pi BSD 72422 PI 8262014 5 OG 04 AM Protocols 129 Current Database Space A Kits o OG Select Manual Archeve to individually archive Ses Backup the entre system by selecting Backup System and restore LIS Records o _ Current Local Archive Space 7 Used 1 i Used System Backup Manual Archive e A Browse
27. er air pressure builds If so air must be leaking from the lines somewhere outside of the Luminex analyzer Contact Luminex Technical Support 26 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide 7 Acquisition Issues a Check to make sure the correct bead set is selected b Pipette the sample up and down to make sure that the microspheres are re suspended c Verify that the sample probe height has been adjusted properly d Try to calibrate the unit if calibration is not successful there may be a clog in the system See Appendix D Basic Instrument Troubleshooting Step 1 Sample Probe Clog e Recalibrate the system If the calibration fails call Luminex Technical Support 8 Software Freezes a Archive the Luminex Database See Appendix I Archive b Defrag computer c Check computer hard drive memory availability if nearing capacity consider emptying hard drive to external storage LABScan3D and xPONENT 4 2 Quick Reference Guide 27 One Lambda Inc A Thermo Fisher Scientific Brand Appendix E Re reading Individual Sample Wells On the Batches tab click tit Create a New Batch from an existing Protocol Enter a distinguishing Batch Name for the samples to be re run Select the same protocol that was used for the first reading Click Next Select the wells to be read and click for Unknown Manually enter the sample IDs in the ID field Press Run _ a new outp
28. er the batch has completed running if any samples need to be re run see Appendix E Re reading Individual Sample Wells or if a batch needs to be replayed see Appendix F Replaying a Batch 5 Once completed click Eject hal and remove the plate 6 Click Retract aay Create a Multi Batch Note In order to create a New Multi Batch pending batches are needed See Create a Batch above steps 1 9 in step 9 click Save and a pending batch will appear in the lower portion of the Batches tab 1 On the Batches tab create a batch for each assay to be run on the Multi Batch and Save 2 Click Create a New Multi Batch 7 3 A Prompt will open see Figure below select the Pending Batch as it should appear on the reading tray then select the OK button Select Pending Batch Instructions Select a pending batch from the list below and click OK fo add that batch to the Mulli Batch list Ifthe batch overlaps with any other batch it will not be added and an error box will be displayed Batches are filtered by plate name and direction Protocol Protocol Version Date Status RSSO2Q 008 001 2 2 2012 4 12 3 Pending RSSO20 008_ 001 2 2 2012 4 12 1 Pending Cancel LABScan3D and xPONENT 4 2 Quick Reference Guide 17 One Lambda Inc A Thermo Fisher Scientific Brand Note Position of placed batches may be moved using the Move button 4 Select the start position of next batch and click the Add button Select the next
29. ibration Performance Fluidics System Verification Venficabon Prep Shutdown Lot Management Cmds amp Routines Command Reagents Calibraton Kit Performance Kit Warmup B35628 B33865 Probe amp Heater Prime None Pending oe Alcohol Flush RB1 70 Alcohol Pending AcoholFiush Vortex each s reagent vial for 30 seconds Piace 5 drops of each reagent into the designated well Fill the designated reservoirs Ss th Di H20 70 isopropanol 10 20 household bleach Info Wash RAI Di H20 Pending ie a applicable to Me chosen routine System w Wash Wash RA1 DI H20 Pending Soak F3DCAL1 SA1 F3DCAL1 Pending 3 Drain R 1 am oon System Status i F3DeCAL 1 SB1 F3DeCAL1 Pending O FIDCALI QO F3DCAL2 SC1 F3DCAL2 Pending lt F3DECAL1 j Ge Schedule F3DCAL3 SD1 F3DCAL3 Pendina oca Or LABScan3D and xPONENT 4 2 Quick Reference Guide 13 One Lambda Inc A Thermo Fisher Scientific Brand Performance Verification Only 1 On the Maintenance tab select the Auto Maint sub tab then select the bubble for Performance Verification a Select the correct lots of the Performance Verification Kit in the Performance Verification Kit pull down menu See Figure below Note Verify that the lot numbers of the performance verification bottles match up to the corresponding kit lot numbers Lot information is located under Maintenance gt Lot Management If a performance verification kit needs to be imported to the LABScan3D software
30. ic Instrument Troubleshooting Step 3 Stringent Clean Routine 24 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide 3 Stringent Clean Routine The unit may have an internal clog that the Remove Clog routine could not resolve a Sonicate the probe for 5 6 minutes in a 15mL falcon tube the narrow end of the probe should be at the bottom of the falcon tube and slightly immersed in 5mm of DI water within the tube b Flush the probe with DI water from the narrow end so the DI water flows out of the larger end c Replace the sample probe and adjust the probe height see Appendix A Automatic Sample Probe Height Adjustment above d Select the Maintenance Tab gt Cmds amp Routines e Create the following routine prime tree times i Prime three times s li Select RA2 reservoir top right reservoir add the Sanitize command twice iii Select RB2 reservoir bottom right reservoir add the Sanitize command twice e iv Backflush hd v Drain into SF1 stripwell O vi Backflush i vii Drain into SG1 stripwell viii Backflush ix Drain into SH1 x Select RB1 reservoir bottom left reservoir add the Alcohol Flush 2 command twice tiss xi Select RA1 reservoir top right reservoir add the Wash command three times o xii Click the Save As Ul button at the bottom name the Routine Stringent Clean it will now be available in the
31. ion respirators and ventilation devices 32 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide Appendix I Archive Note Files will fill up the Luminex SQL database Note Batch output data files are very large in order to maintain software speed regular archives should be performed 1 The Archive Utility will allow the user to schedule archives a Select the Admin tab gt Archive then click Launch Archive Utility Luminex cA Home 4 Samples tu Batches Results gt p gt 0 Gu Qu 7 5 p S Protocols 4 Maintenance de i Admin Archive Instructions Press the archive button to launch the arch ve ubity Group Setup User Setup Batch Options Alert Options You can archive and backup from there Press the button below to launch the archive utility From the archive utility you may archive or restore the system You may setup a schedule for automatic archiving as well as perform manual archives b The Archive Utility will open allowing scheduling an archive be sure to Save after making changes to Schedule Overview c Batches may also be manually archived i e large batches that take up a lot of database space d Select the Manual Archive button Archive Utility instructoms Setup a schedule tor Automatic Reminders or Archives ihe system form previous backup by setectng Restore
32. list is shorter than the number of Unknowns selected the excess Unknowns will still be labeled as UnknownX Luminex amp C Results S Protocols 4 Maintenance 4 Admin d PE amp Step 3 Plate Layout for New Batch vo Instructions onie S 10 add samples and maintena mmands to the plate Edit the sample ID and Dilution factor in the command list and dy direction in which to r a tis anal Groupeng 111222 Ooo Save Prtcl Save Run Batch 16 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide 9 If the batch is going to be run immediately go to Run a Batch Step 1 below If the batch is going to be run later click Save and the batch will appear on the lower portion of the Batches tab until use as a pending batch when it is time to run a saved batch simply click on the pending batch from the Batches tab and go to Run a Batch Step 1 Run a Batch Note Batch output data files are very large in order to maintain software speed regular archives should be performed See Appendix l Archive 1 Click Eject at the bottom of the screen and load the plate to be read Note Check the plate orientation and verify that well A1 is at the top left hand corner of the plate holder 2 Click Retract KA 3 When ready to begin playing the batch click Run Batch 42 at the bottom right hand corner of the screen 4 Aft
33. mport into XPONENT Software ccccccseceeeeeseeeseeeeeeeeees 22 Appendix C Calibration and Verification Bead Information ccceeeee 23 Appendix D Basic Instrument TroubleShooting ccccccecceeeceeeeeeseeeeeeeeees 24 Appendix E Re reading Individual Sample VWells rrrarrranoranrrnnnrnnrennnernnennner 28 Appendix F Replaying a Batch rrrrnrnnnonnnnnnrnannnnrnannnerrnnnnnrnnnnnnennnnnernnnnneenn 29 Appendix G Edit Patient ID Sample ID r rrrnnornnornnennnrnnnenanenanennnrnnnennnennnennnen 30 Appendix H Maintaining FIUGS ccavicinsdcsuseriunconcessstsineraieeiaauinenniasmerseaviainnbennsnniunces 32 Appendix l ea EE 33 Appendix NS ENN 37 Appendix K Support UNN eee 38 LABScan3D and xPONENT 4 2 Quick Reference Guide 3 One Lambda Inc A Thermo Fisher Scientific Brand Powering on the LABScan3D Unit 1 Turn on the computer and the LABScan3D unit LABScan3D power switch is located on the bottom right hand side of the unit Note It takes approximately 30 minutes for the lasers to warm up before being able to Calibrate or Verify the unit 2 Double click the Luminex xPONENT icon bal the xPONENT 4 2 program will open 3 Check the 20 liter Sheath Fluid and Waste Containers if the sheath fluid needs replacement be sure to prime the unit three times after replacement See Appendix H Maintaining Fluids User Computer Screen Saver and Power Saving Settings Must be set before
34. never thumbnails _ Always show menus V Display file icon on thumbnais V Display file size information in folder tips Display the full path in the ttle bar Classic theme only ampiv arves in the Lomputer Olde J Hide extensions for known file types V Hide protected operating system f es Recommended oc C cores to 8 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide LABScan3D CSV Options Settings Must be set before the 1st assay IS run 1 Before running any assays check that the CSV options are set correctly Under the Admin tab go to CSV Options Note Once these settings have been saved DO NOT change them Note To update the License code for a full license select Licensing under the Admin tab a Verify that the following options are checked See Figure below i Automatically export results CSV file when batch is complete ii Automatically export batch when batch is complete ii Include Advanced Statistics iv Automatically export LXB files v Automatically export Run CSV files vi Automatically convert the raw run files to CSV format for each well in the batch b Specify a folder for CSV Output files under CSV Export Folder and Automatically Exported Batch Folder Note This will change the location of the CSV Output files but will not change the location of the Run Files rcsv and Ixb files C Specify a folder for each Raw Run Files Export Option
35. ns Py Location and Other view by Large coms te Category large kom BitLocker Drive Smal cons e Default Programs T Getting started m bag Graphics and ED hail 32 bit In the new window click Personalization from the bottom left corner gt Control Pane All Control Panelltems Display v Contre Panel Home Adpust resolution G Calibrate color Change daplay vettengs Apt ClearType text Set custom text sae DPT Make it easier to read what s on your screen You can change the size of text and other Rems om your screen by cheesing one of these options To temporarily enlarge just part of the screen use the Magnifier tool Smaller 100 defauk Preven Medium 125 Larger 150 8 Select Screen Saver from the bottom right corner In the Screen Saver Settings window change to None click Apply OK and Exit CION gt Control Panel All Control Panel kems Personalization mm Control Panel Home Change desktop icons Change mouse powers Change your account picture Display Taskbar and Start Menu Ease of Access Center lt r Change the visuals and sounds on your computer Click a theme to change the desktop background window color sounds and screen saver all at once Po ia Scenes au i Landscapes Nature United States Installed Thermes 1 Desktop Background Win LtBiue 19204200 Frost Window Color LABS
36. nstructions tt an optonal baih beioe amp you vart particular Gatch mouded n ihe support Me are and you can aiport he uppat IP for hare Name 072514 FMR_TURK A 072414 FM3D RA 0 Probe amp Heater 072415 TUR DR 017 072415 TUR B 017 JC System Info 072415 TUR A 014 JC 072314 LSM 019 JC System Status 071514_DRNO_06_JC 071514 DR O17 JC Schedule 074514 DRAR 01 JC 074514 DROWD 01 JC Philsa DP Ptuksa DQ Priksa CHD Prubsa One Lambda Inc A Thermo Fisher Scientific Brand RSSO1A LS30 014 RSSO1A LS3D 014 RSS0281 L530 017 RSS01B L53D 017 RSSO1A LS30 014 LSM12019 15830 RSSOHZB1 008 RSSO2B1 L530 017 RSSOXZSIR_001 RSSOW251R_001 RSSO2P LS3D 005 RSSO2Q_009 RSSOH1C_005 RSSON1C_005 77252014 6 08 PM oa 125014 408 PM og 1225 2014 350 PU og 125204 3 44 PU T2y2014 8 37 PU Nr 82014 11 09 PM 11162014 11 06 PM 1110 2014 1056 PM 11632014 10 48 PM 001 1115 7014 712 PW 11520114 7 08 PU 115014 7 05 PU 152014 701 PU LABScan3D and xPONENT 4 2 Quick Reference Guide 3 Fill out the User Info designate the location for the Support Utility Output File under Output Directory add any additional files by clicking Add More Files add any screen shots by clicking Take Screen Shot then click Save File the Support Utility Output File will be generated in a zip file at the location designated
37. on Area Icons al Programs and Features QuickTime 32 bit Vig Recovery E Sound ib Speech Recognition w Sync Center A Troubleshooting R User Accounts 3 Windows CardSpace Fay Windows Mobility Center Windows Update LABScan3D and xPONENT 4 2 Quick Reference Guide 3 In the Power options window under Preferred plans select the Balanced recommended or Dell radio button and then click the Change plan settings link to the right mel VE CK Control Panei All Control Panelkems Power Options fl SearchContrlPaned o Control Panel Home Select a power plan Require a password on wakeup Power plans can help you maximize your computer s performance or conserve energy Make a plan active by selecting it or choose a plan and customize it by changing its power settings Tell me more Choose what the power button about power plans does Create a power plan Preferred plans 2 Choose when to turn off the display Automatically balances performance with energy consumption on capable hardware Power saver Change plan settings Saves energy by reducing your computer s performance where possible Show additional plans P gt Control Panel All Control Panel Items Power Options v Search Control Panel L Control Panel Home Select a power plan Require a password on wakeup Power plans can help you maximize your computer s performance or conserve energy Make a plan active by selecting it or choose
38. ples fr Batches rt Results Adjust the sample probe height and plate heater settings Instructions Setect the locations requiring probe height adjusiment To adjust and save the probe height for a plate type a name if the plate is new o Of select a name for existing plates then select Auto Adjust Height e Auto Maint Adust Probe Hegh Plate Heater Lot Management B Piate Type 96 Well Plate h Plate Name plate name here m ON OFF A A z gt Y 1At 7 RAI SAI Probe amp Heater b R amp r H v 2 1 2 5 a amadu AP aif Height e 6 Schedule Support Utility Current height as the Meaght offset value fom Height Meght where he probe was DAWID he DOOM 0 49 0 49 of he Fay He Gsk or fe s here fave Piate ted System Initialization 1 On the Maintenance tab select the Cmds amp Routines sub tab from the left hand side then on the pull down menu for Routine Name select Daily Instrument Startup Luminex See Figure below Luminex Maintenance Commands and Routines Auto Maint Instructions Run single or multiple commands or run a routine Create a routine for the selected commands by clicking Save As Rowtne Name lt None gt z Plate Name Current 96 well plate Commands lt None gt Calibration Verification Luminex Daily instrument Shutdown Luminex lf Daily instrument Startup Luminex LABScan3D
39. r Verifier verifies RP1 standard PMT high PMT and extended range a Do not correlate to known regions on the bead map Fluidics 1 FLUID1 05 Verifies targeting quantity of one known region 2 FLUID2 05 Verifies targeting quantity of second known region Verifies Bead carryover in region of Fluidics 1 LABScan3D and xPONENT 4 2 Quick Reference Guide 23 One Lambda Inc A Thermo Fisher Scientific Brand Appendix D Basic Instrument Troubleshooting Note If needed see the back cover for One Lambda Inc Technical Support Contact Information and Luminex Technical Support Contact Information Note if needed see Appendix K Support Utility for instructions on creating a Support Utility Output File 1 Sample Probe Clog a Sonicate the probe for 3 5 minutes in a 15mL falcon tube the narrow end of the probe should be at the bottom of the falcon tube and slightly immersed in 5mm of DI water within the tube b Flush the probe with DI water from the narrow end so the DI water flows out of the larger end c Replace the sample probe and adjust the probe height see Appendix A Automatic Sample Probe Height Adjustment above d Under Maintenance tab click on Cmds amp Routines on the left hand side e In the Routine Name drop down menu select Remove Clog Luminex procedure f A series of commands will populate under Commands Sequence Backflush 2X Wash Backflush Drain 8X Alcohol Flush 2X and Wash 3X g Click Ejec
40. s i Automatically export LXB files ii Automatically export Run CSV files d Save changes Luminex Ge up XPONENT fr Batches Results S Protocols p Maintenance Ei Admin CSV and Batch Options Instructions Set options for exporting batches to csv files i CSV and Batch Export Options somatcally export results CSV file when batch is complete Test Sort Order By Region ID Curomate at export batch when batch is complete Maximum number of data columns in CSV file 10 Use US regionakzation format only Onn Advanced Statistics CSV File Export Folder and Automatically Exported Batch Folder CSV Options C ProgramData Luminex xPONENT 42 Output Raw Run Files Export Options Archive Aachwve Automatically Export LXB files C ProgramData Luminex PONENT 470utpuk Licensing omatically Export Run CSV fles C ProgramData LuminexixPONENT42 Output Schedule romanica convert the raw run files to CSV format for each well in the batch LABScan3D and xPONENT 4 2 Quick Reference Guide 9 One Lambda Inc A Thermo Fisher Scientific Brand Adjusting the Probe Height for a LABType and LABScreen Plate Must be set before the 1st assay is run Note Each Protocol is created with a plate type built into the protocol based on the assay type A plate type must be saved for both the LABType Assay and the LABScreen Assay Protocols Note The probe height should be re adjusted and saved for all
41. ste fluid container each time the sheath fluid container is replace or filled c Place the waste fluid container at least three feet below the surface on which the LABScan3D instrument rests Do not place the waste fluid container on top of the instrument Do not move the waste line vertically while the LABScan3D instrument is running Contact Luminex Technical Support before relocating the waste fluid container or rerouting the waste line The line may be moved temporarily for cleaning and maintenance 3 To replace the sheath fluid container a Disconnect both the sheath fluid line and the waste fluid line from each of their corresponding containers Careful not to touch the sheath fluid line except for the part that resides outside the sheath fluid container b Place the sheath fluid line into a new container of sheath fluid c Move the empty sheath fluid container to become the new waste fluid container or discard the waste from the waste fluid container in accordance with all local state federal and country specific biohazard handling regulations and replace again for use as the waste container d The sheath line in should be secured tightly to the sheath container e Prime the LABScan3D instrument at least 3 times WARNING Waste fluid can contain bio hazardous infectious agents Follow appropriate biosafety procedures and use personal protective equipment such as gloves gowns laboratory coats face shields or mask and eye protect
42. t System Status Schedule Siinrunrt I Hill 5 Command state is Disconnected a Verify that the outlets have power b Power off the computer and LABScan3D Unit c Check the power cords and communication cable connections d Power on the computer and LABScan3D Unit e Verify that the Firmware is up to date check by selecting the Maintenance tab and then select System Info on the left 6 System Pressurization Normal air and sheath pressure readings are 6 9 psi while the compressor is running If the system is not pressurizing properly verify the following a Check inside the instruments for leaking fluid If large leaks are present contact Luminex Technical Support b Make sure the tubing from the sheath fluid box is securely tightened c Disconnect and reconnect the sheath and waste lines to the unit The connections should click when they are inserted signaling that they are secured Also make sure the fittings are not cracked d Make sure the sample tubing is tightly connected above the sample needle below the blue light e When the Luminex analyzer is turned on does the compressor engage The low humming engine sound at first and then it turns off automatically after the instrument pressurizes If not contact Luminex Technical Support f If the compressor is engaging but pressure is not building within the system disconnect the sheath and waste lines from the Luminex analyzer Run a prime and notice wheth
43. t and fill corresponding reservoirs with DI water and 70 alcohol solution according to reservoir illustration h Click Retract IA i Click Run 22 2 Calibration or Verification Failure a Verify that the probe height is adjusted for the plate being used b Verify correct lot numbers for both Calibration and Performance Verification beads c Verify bottles were properly mixed prior to use at least 30 seconds on the vortex d Verify correct wells have been selected on the XY Platform e Verify that at least 5 drops of the calibration and performance verification reagent beads were used f Verify that there are bubbles in the wells signaling the uptake of the beads if not the sample arm may not have moved check the system pressurization See Appendix D Basic Instrument Troubleshooting Step 6 System Pressurization g If the above steps have been completed verify that there were at least 250 events sec or more during Calibration Verification and the pressure is between 6 9 psi h If the event sec rate is too low there may be a clog in the system See Appendix D Basic Instrument Troubleshooting Step 1 Sample Probe Clog i Rerun the Calibration Verification or the Performance Verification on the system j If the Calibration Verification or the Performance Verification fails call Luminex Technical Support k Luminex Technical Support may request the Stringent Clean Routine be run on the unit See Below Appendix D Bas
44. te Delete Kit Expiration Date Delete Kit l Cmds amp Routines Lot Expiration A x B31091 Probe amp Heater Active Lot Number Expiration Date Target F3DCAL1 631091 11 30 2014 DO 9707 F3DeCAL 1 B30522 9 26 2014 CLI 3431 System Info F3DCAL2 231986 1 17 2015 CL2 3247 CALS 129565 M 7120 2014 GE 3414 System Status FSOCALS B29565 1120 2014 CLS 341 F3DVER1 633195 4 1 2015 aDaAVER 323196 wo 2015 Schedule F3DeVER1 B33196 1 1 2015 F3OVER2 B33807 6 14 2015 Support Utility 22 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide Appendix C Calibration and Verification Bead Information Calibrators 1 F3DCAL1 05 Classification Calibrator for non magnetic beads calibrates DD CL1 CL2 CL3 2 F3DeCAL1 05 Classification Calibrator for magnetic beads calibrates DD CL1 CL2 CL3 3 F3DCAL2 05 Reporter Calibrator calibrates RP1 standard and enhanced range all beads 4 F3DCAL3 05 Extended Dynamic Range Calibrator calibrates RP1 extended range all beads Verifiers 1 F3DVER1 05 Verification Calibrator for non magnetic beads verifies DD CL1 CL2 CL3 a Comprised of 11 known regions of the bead map 6 22 71 81 96 102 153 352 374 378 500 2 F3DeVER1 05 Verification Calibrator for magnetic beads verifies DD CL1 CL2 CL3 a Comprised of 11 known regions of the bead map 6 22 71 81 96 102 153 352 374 378 500 3 F3DVER2 05 Reporte
45. under Output Directory Support Utility About Instructors Select an application on the let 10 view Geteul support Mes Click the Add More Fikes or Take Screen Shor button to add additional support Mes When you are done click Save File 10 generate The suppor We Select Application User Info xPONENT 4 2 Lompary Name One Lambda part of Thermo Fisher Scientific Phone Number Emad 818 449 3379 john cha thermofisher com Meow erter o comment Support Files SOS C ProgramData urmnex OCommon MX Support Support tes 2014728 22844syslemevto txt C ProgramData Appkcaton DatsuPONENT Setup tog C ProgramData Appkcaton Dated UMNE XuPOMENTAN ogs ctartup 1 log C ProgramData Appkcaton Dated UMNEXUPONENTANMN ogs atarnup 10 log C ProgramData Apptkcation Dated UMNE XUPONENT SN ogs startup 2 log C ProgramData Appkcation Dated UMNNE XuPONENTAN ogs startup 3 log C Pro amData Anne Asr Dated MO KG POSE NT EDI As Stan inn e Directory Configuration Output Directory C WsersiFLEXMAP3O Documents Browse Add More Fies Take ScreenShot LABScan3D and xPONENT 4 2 Quick Reference Guide 39 One Lambda Inc A Thermo Fisher Scientific Brand Contact Information One Lambda Inc Technical Support Luminex Technical Support E mail techsupport onelambda com Email support luminexcorp com Telephone 818 449 3230 Telephone 512 381 4397 Toll free 800 822 8824 EXT 3230 Toll free 1 877 785 2323 Fax 5
46. ut file and run files will be created EE ee N a 28 One Lambda Inc A Thermo Fisher Scientific Brand LABScan3D and xPONENT 4 2 Quick Reference Guide Appendix F Replaying a Batch 1 On the Results tab select the Saved Batches sub tab 2 Select the completed batch to replay and then select the Replay button A prompt will open Select Replay Mode select the Replay Batch bubble and then click okay 4 Re name the batch and then select the protocol to use to replay the batch 5 Click Next 6 On the Plate Layout section select by dragging all the wells to be replayed Then select for Unknown 7 Next either click gt Import List to import a Sample ID Import list to the batch or edit the Sample ID of each well by double clicking in the ID field next to the corresponding well position and give each ID a sample name 8 Click the Replay Batch an output file will be created Note The LABScan3D unit does not need to be ON to replay a batch LABScan3D and xPONENT 4 2 Quick Reference Guide 29 One Lambda Inc A Thermo Fisher Scientific Brand Appendix G Edit Patient ID Sample ID Patient ID Sample ID maybe changed after a batch has already completed running 1 Select the Results tab gt Saved Batches 2 Highlight the batch with the Sample IDs that need editing and click Open Completed Batches RSSOH2B1_008 7 15 2014 6 38 PM Complete RSSO2B1_LS3D_017 002 7 15 2014 6 35 PM Complete RSSOW2B1R_001 002 7
47. y updated to the selected plate 1 On the Maintenance tab select the Probe amp Heater sub tab Select the Plate Type from the pull down menu either 96 Well Plate or 384 Well Plate and give the selected plate a Plate Name Note The LABScan3D unit can store the probe height settings for different types of trays or different lots of trays as long as the tray is saved 3 Select the locations on the plate that the probe height will automatically adjust to The height must be adjusted to one plate well one reservoir well and one strip well location See Figure below wells A1 RA1 and SA1 are selected Note the selected well of plate strip well and reservoir must NOT contain any liquid Go em XPONENT LJ Luminex o 4 eS Ka Home Samples Batches Results Protocols oa anenance Admin a Adjust the sample probe height and plate heater settings Auto Maint Instructions Select the locations requiring probe height adjustment To adjust and save the probe height for a plate type a name If the plate is new or select name for using plates then select Auto AGL ist Height Adjust Probe Height Plate Heater Lot Management P Plate Type 96 Well Plate x Reservoir Strip Wells Cmds amp Routines Plate Name plate name here ON OFF N a 7 1At 7 RAI 7 SAI Probe amp Heater t 3 4 5 6 7 8 gt 8 R 1 2 5 1 P 3 T Auto Adjust A 9 Height t t System Info
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