Product Manual
Contents
1. WORLD AN CAUTION Steam coming out of pressure limit valve can cause a SERIOUS HEALTH HAZARD Avoid scalding by wearing suitable protective clothing and exercising extra caution 6 13 Confirm that the red pressure valve dropped and remove the lid by turning it clockwise Open and Lift the working chamber 6 14 Remove the container with the slides from the working chamber Allow slides to cool for 15 20 minutes Proceed with slide treatment as recommended by your protocol Be aware that container and inner parts of cooker will be extremely hot Avoid SERIOUS HEALTH HAZARD scalding or burns by wearing suitable protective clothing and exercising extra care 7 CLEANING AND STORAGE 7 1 Wait until the working chamber the water inside chamber and the trivet have been cooled to a room temperature 7 2 Remove the working chamber from cooker and remove the trivet from the working chamber 7 3 Inspect the main body and remove any water residue from inside or outside with a clean dry paper towel rug Do not immerse the main body in water or pour water onto it 7 4 Dispose the water from the working chamber and dry the working chamber and trivet with clean paper towel rug Never use harsh chemicals or scouring pads 7 5 If needed disconnect the condensation collector remove any water and dry it with clean paper towel rug 7 6 If needed dry the lid and the gaskets with a clean towel rug Never use harsh chemicals or scouring pads 7 7 C2. body Any liquid inside the main body may cause a malfunction with permanent damage as well as cause a SERIOUS HEALTH AND OR FIRE HAZARD 6 2 Position the trivet the inside working chamber legs down and pour approximately 500 ml to 1000 ml of water into the working chamber to barely cover the trivet s surface 6 3 Prepare the reagent holder BSB 7009 with slides in slide rack BSB 7010 and gently fill the container with working concentra tion of HIER solution We recommend Bio SB ImmunoDNA Retriever with Citrate BSB0023 BSB0020 BSB0021 BSB0022 or with EDTA BSB0033 BSB0030 BSB0031 BSB0032 6 4 Place the prepared container inside the working chamber on top of the trivet Make sure that the bottom of the container makes good contact with water in the working chamber 6 5 Place and secure the lid onto the cooker s main body This is done by putting the lid into place atop the cooking chamber with the red pressure valve on the left and turning counter clockwise to secure in place 6 6 Position the pressure limit valve into the pressure position see below figure Figure 6 Pressure cooker in Figure 7 Pressure cooker in pressure mode pressure release mode www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB 9 BIOSCIENCE FOR THE WORLD 6 OPERATION PROCEDURES Continued 6 7 Plug the power cord into an outlet 6 8 Press the MENU button on the control panel until the desired
3. settings that can be used for deparaffinization epitope retrieval or nucleic acid retrieval Time ISH This section will focus on tips and recommended settings for the TintoRetriever Low Pressure High Pressure O in a clinical or research setting L eu 100 C 100 112 C IHC Epitope Retrieval amp ISH Nucleic Acid Retrieval 80 86 C 58 62 C For formalin fixed paraffin embedded FFPE tissue sections the High Pressure setting with a time of 15 minutes is recommended for thorough heat permeabilization of tissue We recommend using Bio SB Immuno DNA Retriever Figure 10 Pre programmed temperature with Citrate or Immuno DNA Retriever with EDTA for IHC ISH protocols settings Tissues prone to detachment Should tissues detach after using the High Pressure setting it is recommended that tissues are mounted using Bio SB Hydrophilic Plus Slides BSB 7028 Hydrophilic Plus Slides prevent tissue detachment while promoting reagent dispersion Tissue Microarrays Figure 11 Hydrophilic Plus Slides BSB 7028 Tissue Microarrays or TMAS typically need gentler epitope retrieval methods than those used with whole tissue sections At Bio SB our 11 core NH TMA BSB 0297 and 23 core NH TMAS BSB 0298 undergo epitope retrieval using the 100 C Setting Additionally the Bio SB TintoRetriever PT module has gentler epitope retrieval settings BSB 7030 and may be ideal for labs that utilize TMAS Deparaffinizati
4. Bio SB BIOSCIENCE FOR THE WORLD Bio SB TintoRetriever Pressure Cooker Nucleic Acid amp Epitope Retrieval System Bio SB Inc 69 Santa Felicia Drive Santa Barbara CA 93117 USA Tel USA 1 800 561 1145 Tel Int 1 805 692 2768 Fax 1 805 692 2969 Bio SB gt BIOSCIENCE FOR THE WORLD Index Topic Page Description amp Features Page 2 Main Technical Parameters Page 3 Safety Reguirements Page 4 Parts amp Features Page 5 Installation Page 6 Operating Procedures Page 7 Cleaning amp Storage Page 9 Temperature Settings Page 10 Applications for IHC Page 11 Troubleshooting amp Contactinfo rr Pagel2 Figure 1 TintoRetriever system with slide racks and OC Certificate Page _ reagent holders 1 DESCRIPTION amp FEATURES Most formalin fixed paraffin embedded tissues require an antigen retrieval step prior to any immunochemical staining The antigen retrieval step breaks some of the methylene bridges that cross link proteins in formalin fixed tissue and allow antibodies to bind to unmasked epitopes Heat induced epitope retrieval and nucleic acid retrieval steps are most often performed using a conventional pressure cooker or a mi crowave oven The Bio SB TintoRetriever is a precision controlled heat source capable of maintaining a constant and reliable temperature while minimizing the potential for evaporation of the working solution under HIER procedure conditions The TintoRetriever Pressur
5. densation Collector Handle Main Body Heating Plate 1 2 3 4 5 6 8 9 Base Power Cord Lid Handle Lid Sealing Ring Supporting Cover Rubber Grommet Upper Ring Control Panel Trivet Figure 3 TintoRetriever Parts www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB 9 BIOSCIENCE FOR THE WORLD 5 INSTALLATION 5 1 Remove TintoRetriever Pressure Cooker from packaging material and inspect for any damage Be sure all parts have been included before discarding any packaging material If any signs of mechanical damage to the Digital Pressure Cooker have been observed do not use it and contact Bio SB see page 12 for contact details 5 2 Thoroughly clean all parts with a towel rug and Place the Digital Pressure Cooker on a clean flat surface 5 3 Place the condensation collector in the rear of the main cooker body until it clicks into place as shown below NOTE Even though pressure limit valve will have a loose fit it is safely secured Figure 4 Condensation Collector Placement Place the pressure limit valve on the lid as shown below Figure 5 Setting Instrument to Pressure setting www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB 9 BIOSCIENCE FOR THE WORLD 6 OPERATION PROCEDURES 6 1 Place clean and dry working chamber and dry the main body Make sure that no water or any other liguid is present inside the main
6. e cooker is recommended for use in both Immunohistochemistry IHC and In Situ Hybridization ISH protocols TintoRetriever Applications Pictured on Cover Rapid Heat Retrieval Multiple Temperature Settings Use in IHC amp ISH Applications Low Reagent Use Holds 96 Slides Per Run FISH i p40 RMab ALK EML4 TriCheck HER2 CEN 17 Cost Effective HIER Solution BSB 2072 Z 2117 200 3022 40 www biosb com email info biosb com phone 1 805 692 2768 2 V3 08 04 14 Bio SB 2 BIOSCIENCE FOR THE WORLD 2 MAIN TECHNICAL PARAMETERS Power Requirements AC110V 60Hz 1000W Dimensions 320mm x 320mm x 340mm W x D x H Packaged Dimensions 330mm x 330mm x 360mm W x D x H Weight 12 6 Ib 5 7 Kg Operational Temperature 60 C 121 C Operational Humidity 85 Chamber Working Temperature 121 C Chamber Working Pressure 15 PSI 103 kPA 1 03 bar 1 kg cm2 NOTE TintoRetriever Pressure Cooker is intended to be run with AC110V 60Hz 1000W If there is a need to operate this instrument at AC 220V Bio SB recommends using a transformer with the following specifications Output 110Volts and a minimum of 1000Watts STEP UP amp DOWN TRANSFORMER LifeFuze 1000 Figure 2 Recommended transformer for use in countries with 220V standard electricity www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB 9 3 SAFETY REQUIREMENTS AN CAUTION Read the user manual fully be
7. es strict quality control procedures and that every TintoRetriever unit is delivered defect free Should your TintoRetriever malfunction or otherwise fail under normal use within the first year of ownership Bio SB will replace the unit at no charge Product Name Bio SB TintoRetriever Pressure Cooker Product Catalog Number BSB 7008 Serial No This product is tested and qualified for delivery Checked by Date www biosb com email infoobiosb com phone 1 805 692 2768 B V3 08 04 14
8. fore use and pay particular attention to sections containing this symbol Please care fully read user manual before installation operation maintenance and repairing to avoid personal injury and damage to the Instrument 3 1 Do not immerse electrical cord plugs or main cooker body into water or other liguids to avoid electrical shock and or fire hazard 3 2 Do not touch hot surfaces of the pressure cooker Use handles only 3 3 Operate the pressure cooker only with properly closed lid 3 4 Do not fill working chamber with more than recommended volume of water 3 5 Never attempt to open the lid while the unit is operating Wait until the unit is switched off and pressure has been completely released If the lid is difficult to rotate this indicates that the working chamber still has excess pressure 3 6 Never move the pressure cooker while operating it 3 7 Exercise care when releasing excess pressure from the cooker improper technique may result in scalding or injury 3 8 Unplug from outlet when not in use or cleaning servicing 3 9 Do not use under hanging shelves cabinets etc or damage may occur 3 10 Always check the pressure limit valve for possible clogging before each run www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB 9 BIOSCIENCE FOR THE WORLD TintoRetriever Components Number Component Pressure Limit Valve Red Pressure Indicator Push Rod Sealing Ring Working Chamber Con
9. indicator light is lit Low Pressure High Pressure LU 1067 1107 C 114 121 C 6 9 Press the TIME button until the desired time is lit Bio SB recommends RI a 15 minute time setting for proper epitope or nucleic acid retrieval see 100 C 100 112 C page 11 E Oe I 80 86 C 58 62 If needed press the START CANCEL button twice to reset timer and then set new desired time NOTE Timer will count time accurately only if used with 60 Hz electrical supply 6 10 Press the START CANCEL button to start the procedure The red pressure light will flash As the pressure and temperature increases the red pressure valve on top of the lid will rise and stay in place until the pressure is eventually released START CANCEL TintoRetriever 6 11 Atthe endofthe set time the unit will beep and automatically Heat Retrieval System switch to the keep warm setting until the START CANCEL button is pressed again see 6 8 NOTE The timer will start counting down only after unit reaches the preset temperature and pressure NOTE Do not keep slides under keep warm conditions for an extended amount of time as it will affect performance of your nucleic acid epitope retrieval procedure 6 12 Position the pressure limit valve into the release position Figure 9 Valve in Pressure Release position www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB 9 BIOSCIENCE FOR THE
10. on Hot Rinse Tinto DP1 amp 2 For non toxic deparaffinization and hot rinsing of slides it is recommended Figure 12 Tissue Detachment on a you use the 58 62 setting on the TintoRetriever We recommend Hydrophobic Slide Left vs Hydrophilic Slide TintoDeparaffinator 1 amp 2 BSB 0134 BSB 0137 as a substitute for Xylene and Right aclohol based deparaffinization www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB BIOSCIENCE FOR THE WORLD 8 TROUBLESHOOTING Problem Cause Solution temperature to drop Steam escapes from the rim of lid Red pressure valve will not rise Pressure limit valve is not placed properly Set the pressure limit valve to Pressure setting I Not enough water in the working chamber Add sufficient amount of water to cover trivet Steam escaping from rim of lid while Lid not secured and in lock position Re secure lid and twist until locked pressure limit valve is in Pressure position Serious malfunction Stop operation of unit and call customer support To contact Bio SB customer support Phone International 1 805 453 6631 Phone United States 1 800 561 1145 Email info biosb com Hours of Operation 9am 5pm PST Monday Friday www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB 9 BIOSCIENCE FOR THE WORLD Quality Control Certificate Bio SB ensures that every TintoRetriever undergo
11. onnect the condensation collector back to the main body place the working chamber back inside the main body place the trivet inside the instrument and place lid on top of the main body Protect from heat water snow mechanical and electromagnetic shock NOTE Do not lock the lid to allow any residual moisture to escape internal parts of cooker www biosb com email info biosb com phone 1 805 692 2768 V3 08 04 14 Bio SB 9 BIOSCIENCE FOR THE WORLD 8 TINTORETRIEVER TEMPERATURE SETTINGS High Pressure 1149 121 Low Pressure TET temperature CO 4 Temperature C O de Pressure Ii Pressure 114 121 C 106 110 C Pressure Pressure 106 112 C 106 112 C Temperature C o Pressure Release Pressure 100 C 106 112 C l 104 110 C Temperature C 104 110 C Pressure Release Pressure 100 C 104 110 C 80 86 C Temperature C Pressure Release Pressure 80 86 C 80 86 C 58 62 C Temperature C Pressure Release Pressure 58 62 C 58 62 C Pressure Release Pressure www biosb com email info biosb com phone 1 805 692 2768 V V3 08 04 14 Bio SB BIOSCIENCE FOR THE WORLD 9 TINTORETRIEVER IN THE LABORATORY B 1 O SB e 1IOBCIENCE FOR syr WORLI e The TintoRetriever is a rapid epitope and nucleic acid retrieval system which can be used in a variety of applications in the modern pathology lab The Bio SB TintoRetriever comes pre programmed with 6 temperature
Download Pdf Manuals
Related Search