UNICORN® OS version 1.10 Upgrade Information
Contents
1. AMERSHAM B O S C ENCES UNICORN OS version 1 10 Upgradelnformation This document describes the new features of UNICORN OS version 1 10 and how to upgrade from older versions 1 New features The major changes between versions 1 00 and 1 10 of UNICORN OSare G eneral This version is adapted to make UNICORN OS smoother to work with This version can handle up to 250 blocks in one met hod instead of 130 The LogBook can now collect 12 000 lines instead of 4 096 This change also includes View Export and Print of the LogBook 12 000 lines corresponds to approximatly 90 100 cycles depending on how many instructions there are in a cycle M ethod Editor In the sequence page in Run Setup the CREATE but ton now 1 Creates the method 2 Updates the variables 3 O pens the SaveAs method dialog box 4 Saves the sequence with the displayed name The CREATE button also checks the sequence for double assigned amidite positions on the instrument This occurred on line before but is now done when this button is pressed The 3 base is not included It is only checked to be a possible Support base A C G T a C g u Blocks in Restore mode Calling blocks that are set to Restore mode instead of Normal mode may cause malfunction of some instructions in the called block Even if they appear in the LogBook as executed the system does not per form these actions Furthermore when running a method contai2. Watch _ efficiency replaces the Efficiency Threshold The Efficiency Threshold has been removed from System Settings Instead the new Watch_Efficiency has been placed in the Instruction Box When pro gramming a method the efficiency value is set in the method The watch can be set during a run using M anual Alarm amp M onitor Watch_Efficiency on off and will be on off through out the run P50Gain this instruction is updated to avoid alarms occuring when high flow rates are used in combina tion with a P50 Gain value gt 1 0 CT_Coupl this instruction is updated so it will cal culate the correct contact time It is only used with Flowthrough coupling of the amidite TwoPoint Calibration the pressure calibration routi ne is now a TwoPoint calibration to improve the accuracy of pressure readings from the P 6000 pumps CondP the display of conductivity in percent is no longer included Flowpath ACN_T has changed name to ACN_T U Recycle the Recycle instruction now works with a linear flow rate cm h instead of a volumetric flow rate ml min H elptext helptext has been added to the strategy Amersham i e Biosciences Help for Pump Flowpath and Alarm amp M on instructions is available by pressing F1 2 Upgrading When upgrading from an older UNICORN OS ver sion your files will automatically be updated H owever it is always a good idea to make back ups of your result and method files If you have a ba
3. ck up facility like a Tape station or a M agnet O ptical drive use that to back up your files Otherwise make a copy of the catalog and save it under a different name as shown in the examples below UNICORN OS V1 00 Local Installation 1 Doubleclick on the O S 2 System icon on the desktop then on the DRIVES icon and finally on the DRIVE C icon 2 Doubleclick on the UNICORN folder and then on the LOCAL folder 3 Put the cursor on the FIL folder click the right mouse button and select copy 4 Change the name to UNI_FILES 5 Click on the DRIVE C icon 6 Click on the COPY button UNICORN OS V1 00 N etwork Installation Firstly find out where your methods and result files are stored 1 Doubleclick on the O S 2 System icon on the desktop then on the DRIVES icon and finally on the icon where is the drive where your result and method files are stored 2 Doubleclick on the UNICORN folder and then on the LOCAL folder 3 Put the cursor on the FIL folder click the right mouse button and select copy 4 Change the name to UNI _ FILES 5 Click on the icon where is the drive where your result and method files are stored 6 Click on the COPY button Installation The following procedures describe how to upgrade from version 1 00 to version 1 10 without changing system configuration for both stand alone computers and those in networks Stand alone 1 Doubleclick on the O S 2 System icon on the des
4. ktop then on the DRIVES icon and finally on the DRIVE C icon 2 Doubleclick on the Drive C icon in the Tree View to see the files under c 3 Put the cursor on the STARTUP CM D icon click the right mouse button and select Delete 4 Restart by clicking with the right mouse button on the O S 2 desktop and choose Shut down from the menu When the shutdown procedure is complete turn the power off and on again to restart the computer 5 Insert UNICORN OS disk 1 in drive A 6 Doubleclick on the O S 2 System icon on the desktop then on the DRIVES icon and finally on the DRIVE A icon 7 Doubleclick on the INSTALL icon The installation program will start N ote If this fails open a Command Window and type A IN STALL 8 M ake sure that Program and System are checked If Templates and Strategy are going to be updated check them too 9 Answer the other questions that appear Note The UNICORN OS installation program will suggest the same type of installation as before N ormally all you need do is click OK to these ques tions 10 The installation will now start Change disk when prompted to do so 11 A message appears on the screen when the installation is complete Remove the disk but do not restart the computer yet 12 Restart by clicking with the right mouse button on the O S 2 desktop and choose Shutdown from the menu W hen the shut down procedure is complete turn the power off and on again to restart the comp
5. ning too many calls to blocks set to Restore mode the run stops after a while and the message Error in met hod system stopped appears The number of calls allowed varies with the method used Action Do not use Restore mode in blocks Evaluation The on line Peak integration function is improved to show the maximum PeakH eight and not just the first peak height during the on line integration If the peak is split it will no longer cause false PeakH eight in the Detrit Table 56 3055 34 Edition AA The Detrit Start has changed name to Retention The Efficiency Threshold has been removed from the Detrit table This is because the threshold is now a Watch instruction in the method editor and not a parameter in System Setting N ote Results created with UNICORN OS version 1 00 will display both Retention time and volume as 0 00 since they are saved as Detrit Start values and not as Retention Deleting chromatograms An operating system error message SY 3175 will appear if you run an evaluation procedure where the DELETE_CHROM instruction is used for deleting the same chromatogram that the procedure is run on Action Quit UNICORN and reboot the computer Generally do not use the DELETE_CHROM instruction to delete the same chromatogram that the procedure is run on Strategy Pause_at_Cycle End will pause the run at the next Cycle Start in the method It will be execu ted just once
6. o is click OK to these ques tions 12 13 14 15 16 17 18 19 The installation will now start Change disk when prompted to do so A message appears on the screen when the installation is complete Remove the disk but do not restart the computer yet Double click on the OS 2 System icon on the desktop then on the DRIVES icon and finally on the DRIVE C icon Double click on the Drive C icon in the Tree View to see the files under c Put the cursor on the STARTUP OLD icon click the right mouse button and select Settings Click on the General tab change the title to STARTUP CM D put the cursor on the title bar icon and double click Restart by clicking with the right mouse but ton on the OS 2 desktop and choose Shut down from the menu When the shutdown procedure is complete turn the power off and on again to restart the computer Installation is complete Amersham e Biosciences
7. uter 13 Installation is complete N etwork N ote It is important that all computers in a network using the ame UNICORN OS server have the same UNICORN OS version installed 1 Doubleclick on the O S 2 System icon on the desktop then on the DRIVES icon and finally on the DRIVE C icon 2 Doubleclick on the Drive C icon in the Tree View to see the files under c 3 Put the cursor on the STARTUP CM D icon click the right mouse button and select Settings 4 Click on the General tab change the title to STARTUP OLD put the cursor on the title bar icon and double click 5 Restart by clicking with the right mouse but ton on the OS 2 desktop and choose Shut down from the menu When the shutdown procedure is complete turn the power off and on again to restart the computer 6 Log on to your network and map your UNI CORN OSdriveto the UNICORN OS server See Chapter 12 in the User M anual 7 Insert UNICORN OS disk 1 in drive A 8 Doubleclick on the OS 2 System icon on the desktop then on the DRIVES icon and finally on the DRIVE A icon 9 Doubleclick on the INSTALL icon The installation program will start Note If this fails open a Command Window and type A IN STALL 10 M ake sure that Program is checked Do not select any other option Click on OK 11 Answer the other questions that appear Note The UNICORN OS installation program will suggest the same type of installation as before Normally all you need d
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