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1. Quality Guarantee e Tanner Scientific will ensure that every instrument sold has been strictly examined to ensure it meets our stringent quality and technical standards e Service terms are only provided for those who regularly use the instrument and operate the instrument according to its instructions Disposal of Unwanted Instruments Discarding the instrument and its parts should be done according to current laws and regulations Tanner Scientifice TN5000 Titan Manual Rotary Microtome 11 8 Troubleshooting Manual Rotary Microtome PROBLEMS CAUSES ELIMINATION Uneven slices inconsistent cuts Wrong blade angle Specimen not firmly locked in the specimen clamp and or the blade holder Adjust the angle of cutting until the appropriate angle is acquired Check and firmly lock the screw ofthe specimen lock and the blade holder Slices are compressed or corrugated or extruded Chatter Blunt blade Use another section of the blade or change blade for a new one Use another section of the blade Blunt blade or change blade for a new one Specimen too hot Cutting angle too big Cutting too fast Freeze the specimen on the freezing platform Adjust the cutting angle Turn the hand wheel slowly Nicks or chatter marks are left on the slice Cutting too fast Cutting angle too big Specimen holder or clam2. Paraffin Sectioning Attention Be sure to lock the hand wheel and cover the edge of the blade with the blade protector when not in use e Firmly clamp in the specimen before installing the blade e Putthe pre frozen paraffin block onto the specimen clamp Attention Be sure to carefully avoid the edge ofthe blade itis very sharp e Turn the hand wheel to move the specimen to the back utmost position e Insert the blade into the blade holder and firmly clamp it in e Adjust the cutting angle start at 09 39 e Move the blade holder toward the specimen e Adjust the specimen s surface position so it is parallel to the blade s edge e Loosen the hand wheel Attention Be sure to turn the hand wheel evenly in the course of cutting e Turn the hand wheel to starttrimming e Selectthe ideal thickness e Evenly turn the hand wheel clockwise to cut Tanner Scientific TN5000 Titan Manual Rotary Microtome 9 6 Cleaning Maintenance Cleaning Attention REMOVE THE BLADE e Be sure to lock the hand wheel before cleaning e Brush off any residue with a dry brush e Remove the blade base and the blade holder for cleaning e Do NOT use detergent containing acetone and benzene to clean the instrument it will damage the paint on the surface of the instrument e Only mild cleaners or gentle lye should be used for cleaning the instrument Maintenance Preventive maintenance is needed to ensure a long working life For reg
3. User Manual TN5000 Titan Manual Rotary Microtome Read Prior to Operation Always keep this manual near the unit i TANNER scientific ne cade TANNER 888 708 5233 scientific User Manual TN5500 Titan Info TannerScientific com www TannerScientific com Contents 1 General Summary uu Page 2 2 Safety 3 3 Technical Parameters 4 4 Installation 5 5 Operation 8 6 Cleaning amp Maintenance 10 7 Warranty amp Service 11 8 Troubleshooting 12 Tanner Scientific TN5000 Titan Manual Rotary Microtome 1 1 General Summary Tanner Scientific Titan 5000 Manual Rotary Microtome 6 7 1 ol 2 8 2 033 A id 3 o 5 10 1 Standard Specimen Clamp 2 Course Feed 3 Enclosure 4 Trimming Lever 5 Lock Lever 6 Display Window 7 Section Thickness Knob 8 Hand Wheel 9 Pressure Plate 10 Blade Holder Assembly The Model Titan 5000 is a manually operated microtome powered by rotating the hand wheel The guidelines for the vertical and horizontal movement of the specimen are seamless and maintenance free The specimen advances when the hand wheel is turned 2 Tanner Scientific TN5000 Titan Manual Rotary Microtome 2 Safety Attention Remember to lock the hand wheel and attach the blade protector before operating or changing the blade a
4. 4 Tanner Scientific TN5000 Titan Manual Rotary Microtome 4 Installation 1 Unpacking Unpack the wood crate take out all components and the instruction sheets Important The Titan 5000 Microtome is packed in a safe crate designed to limit any movement of the unit during shipping Warning A hex screw is used to secure the unit to the base ofthe wooden crate Care must be taken to remove the head hex screw from the base before attempting to remove the unit from the crate Hold the base ofthe instrument and remove Do not try lifting the unit by any part of the enclosure at any time Attention Avoid grasping the handle ofthe hand wheel the wheel itself or any part of the section adjustment knob in the course of moving the instrument 2 Instrument Setting e The instrument should be set up on a very stable platform in the lab Requirements for Handling e The unit should always be stable and not allowed to rock on the surface e There should be no vibration coming from the floor e Leave enough room for convenient operation ofthe hand wheel and the wheel s rotation Tanner Scientific TN5000 Titan Manual Rotary Microtome 5 Installation of Specimen Clamp The adjustment mechanism of the specimen clamp has been installed onto the mainframe and the component setting has NOT been preset The mechanism requires installation and adjustment at the time of unit setup Standar
5. the serial number and manufacturing date of this instrument Distributed by Tanner Scientific 1073 Tallevast Rd Sarasota Florida 34243 888 708 5233 Info TannerScientific com www TannerScientific com 8 TANNER scientific 1073 Tallevast Rd Sarasota Florida 34243 888 708 5233 Info TannerScientific com www TannerScientific com
6. Scientific TN5000 Titan Manual Rotary Microtome 7 5 Operation Section Thickness Section thickness range can be adjusted by turning the knob located on the right upper side on the front ofthe microtome The thickness can easily be adjusted using the knob 2 e Section Thickness Range 0 60um e Setting Value O 20um increment 1um 20 60um increment 5um e Display Window 1 shows section thickness range Hand Wheel Operation Attention Always lock the hand wheel before cleaning Quick Locking Lever for Hand Wheel The hand wheel can be locked at any position with the locking lever 3 located at the right side ofthe microtome pedestal The two positions Up Lock Down Loosen of the locking handle are marked on the pedestal e Pull the locking lever 3 Up to mechanically lock the hand wheel a the hand wheel can then not be turned Y e Pull the locking lever 3 Down to loosen the hand wheel the hand wheel can then rotate a Small Hand Wheel The small hand wheel on the left is used to move the specimen clamp forward or backward quickly Turn the hand wheel clockwise to make the specimen move toward blade The hand wheel is designed to move the specimen horizontally and to quickly move the block toward or away from the blade When the hand wheel moves to the foremost and backmost position it will become harder to rotate Attention Be careful to ensure that the specimen is mounted securely on the
7. d Specimen Clamp Lock the hand wheel There is a coattail holder on the standard specimen clamp 1 Loosen the clamp handle 4 Insert the coattail holder leading into the coattail slot 3 When completed lock up the handle 4 Turn the locking handle 7 downward to loosen The position of the specimen clamp can be adjusted by screws 5 and 6 Attention The screws 5 and 6 should be adjusted simultaneously within their range of movement Screw 5 is for Up Down Screw 6 is for Right Left In the course of adjustment the locking handle 7 should be loosened Once the position ofthe specimen clamp is fixed turn the locking handle upward 7 to lock it down To change the Specimen Clamp loosen the locking handle 4 to take outthe specimen clamp from the coattail slot and install new one by reversing the procedure Universal Cassette Clamp e Lock the hand wheel e Installation and adjustment is the same as the standard clamp above e The general specimen should be positioned horizontally or vertically as shown above e Pull the locking lever 2 toward you to loosen mechanism e Horizontally or vertically put in the specimen cassette e Allow spring to retract back to lock cassette in place 6 Tanner Scientific TN5000 Titan Manual Rotary Microtome Installation of Accessories Attention Please don t inse
8. nd at any time when the microtome is not in use The blade protector will cover the blade and protect against cuts Transportation and Installation e Always transport in an upright position e Never pick up by the handles ofthe hand wheel or the knob used for adjusting the section thickness e Do NOT remove or change the protecting devices equipped on the instrument and its components e Pay careful attention to the technical parameters Operation e Be very careful when using blades or disposable blades The sharp edges can cause serious injury e Firmly clamp the specimen before installing the blade e Remember to lock the hand wheel and cover the blade with the protection guard before changing the specimen and at any time that the unit is not being used e Be careful when using any liquids around the instrument as spillage can cause serious damage and void the warranty Tanner Scientific TN5000 Titan Manual Rotary Microtome 3 3 Technical Parameters Section Thickness Setting Range 0 60um Setting Values 0 20uym in 1um increments 20 60um in 5um increments Precision Error 5 Trim Setting Mode 15um or 35um Specimen Retraction Range 90um this function can be shut off Object Feed 30mm Vertical Stroke 70mm Specimen Orientation System 8 X Y moving range of Holder Base Front Back 2 56 Left Right 1 57 Dimensions 19 69 x 22 45 x 11 80 Height of Blade Edge 4 13 Net Weight 73lbs
9. p not locked tightly Turn the hand wheel slowly Adjust the cutting angle Check and firmly lock the screw of the specimen locking device and blade holder or tighten the locking handle 12 Tanner Scientific TN5000 Titan Manual Rotary Microtome TANNER scientific Information The information notes and figures that appear in this user manual represent the present state of knowledge as we comprehend it Due to continuous improvements in technology and manufacturing techniques specifications manufacturing procedures may change For inaccurate statements drawings and technical illustrations in this user manual we exclude liability as far as permissible No liability is accepted for any monetary loss or consequential damage caused by or related to compliance with statements or other information in this user manual Tanner Scientific will update the manual as needed according to the latest technological developments This document is protected under copyright laws Any reproduction of illustrations or text requires prompt documented consent by Tanner Scientific Statements drawings illustrations and other information regarding contents or technical details of the present user manual are not to be considered as warranted characteristics of our products These are determined only by the contract provisions agreed between us and our customers Refer to the name plate on the back of the unit for
10. rt the disposable blade before the blade holder is securely and properly installed on the microtome The disposable blade must be removed before dismantling the blade holder for any reason Installation of Blade Holder and its Base e Loosen lever 9 move the blade holder base 5 backward to V block on the pedestal then lock lever 9 e Loosen the screw 7 install part 8 onto part 5 and adjust the angle of part 8 The appropriate angle is 8 e The screw 7 should be loosened before adjusting the cutting angle e Loosen the locking lever 4 and move part 6 to the left or rightto adjust blade position The blade holder can be used for both low and high profile disposable blades e Turn and loosen the locking lever 1 and insert the blade into the groove edgeways and lock the locking lever 4 after putting the blade into place The protection lever on the disposable blade holder includes a red adjustable handle 2 and pin 3 Cover the blade with the protection lever at any time when not operating the microtome When changing the blade loosen lever 1 and press pin 3 to take outthe blade Attention Remember to lock the hand wheel and cover the blade with the protection fitting before changing the specimen and during times when microtome is not operating Installation of Waste Tray e Position waste tray parallel atthe bottom of base of machine until the tray stops against the base of the microtome Tanner
11. specimen clamp Damage will be done to the blade if the blade contacts the metal specimen clamp 8 Tanner Scientific TN5000 Titan Manual Rotary Microtome Trimming a 4 e Trimming lever 4 located at the left side ofthe machine When in the middle position trimming thickness is 15um When in the bottom position trimming thickness is 30um e First move the blade holder and base close to specimen but don ttouch or allow them to touch Then fasten the blade holderto begin the trimming and sectioning process e When specimen is in the upper position press the trimming leverto accomplish trimming or until the surface ofthe specimen is in a suitable position to start sectioning Sectioning Attention Be sure to turn the hand wheel evenly The speed of hand wheel rotation should match the rigidity ofthe specimen The section thickness value can be adjusted using the knob on the right ofthe machine e Choose the best cutting angle If the specimen is too hard to cut the cutting angle should be increased until a desired ribbon is achieved e The scale to the right side of blade holder is used to adjust cutting angle e Rotate section hand wheel smoothly and evenly to get an ideal section Retraction e The button for the retraction function is located on the back of the machine e Use a screwdriver to turn the button to ON meaning retraction function is activated If turned to OFF retraction function is shut off ON OFF
12. ular maintenance follow the advice listed below e Have an authorized microtome technician service the instrument at least once a year e After the warranty period ends a successive maintenance contract should be made to ensure the service continues Details can be obtained by contacting the Tanner Scientific Service Department e Clean the microtome daily e Lubricate the moving parts monthly with lubricating oil one or two drops is enough e Maintenance should be done by the authorized technicians Self repair could void the warranty and can cause extra expense later 10 Tanner Scientific TN5000 Titan Manual Rotary Microtome 7 Warranty amp Service Tanner Scientific warrantees this product for a period of one year from the date of purchase provided that the customer complies with the rules ofthis manual Extended warranties are available Contact Tanner Scientific at 888 708 5233 for more information Tanner Scientific will not be responsible for the damage and other problems caused by abuse and misuse of the instrument Service Information If you require any service or parts during the warranty period please contact Tanner Scientific at 888 708 5233 or the sales agent who sold you the unit Priorto calling please have the instrument model and serial number ready Amendment Tanner Scientific reserves the rightto change the technical parameters of any model for improving the function of our instruments
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