Incubators - St. Louis Community College
Contents
1. 491 3 427 Eois evo Seo re 1500 2 306 20 LL ee er E ccce Mr 1000 828 80 8 85 82 8 8 8 9 8 828 8 8 8 8 9 8 8 8 8 8 8 8 8 8 8 8 8 8 8 18 8 8 8 08 8 8 8 8 82 868 8 828 8 8 8 8 8 8 8 8 8 8 8 87 8 8 28 8 8 8 28 28 8 8 80 8 8 8 8 8 8 8 8 5 8 9 87 1 8 5 8 8 2 9 8 8 b 010 10 F Email reiser e amp esusim nrl navu mil 20 30 40 50 ou an 100 110 120 130 140 150 Of Rated Fill STLCC_Urvo murnisun 152 15 Spare Tank Main shut off valve Clockwise CW to shut valve off CCW to open Regulator Valve Brass Fixture and Coupling Nut m Regulator shutoff valve to Incubator turn CCW to full open position for normal operation Turn CW to shut off supply completely Regulator Valve Turn CCW to decrease pressure to incubator Adjust to about 12 psi for normal operation OUTPUT TO INSTRUMENT Tank pressure meter to incubator supply line Adjust to about 12 psi pressure maximum SUPPLY TANK PRESSURE Raw tank pressure near 900 psi 1 when full stays near this during use due to evaporation When it starts to drop supply tanks is near empty Page 46 STLCC CPLS Morrison 2 19 2013 Incubator CO2 Changing Tank2. CO2 Tanks amp Regulator m Refrigerated efi rn Isotemp FV Shaker BRDG p ShakerQFV 2 BRD ss a Chicago JE LabLine FV LabCompanion FV 30 cuft Precision Precision 30M Incubator Lab Li ne 40 cuft w CO2 Trophomax Imperial 28cuft 5 6146 Incubators FV Perci Detect on Instrument Picture to Jump to that SOP ercival Plant Prepared by Bob Morrison Diurnal BRDG CPLS Instrumentation Specialist Oct 2008 Latest rev Oct 2012 Hybridization Microwave Countertop Page 1 S PERCIVAL Bench Oven 2cuft Gravity UV Crosslinker 9 CPI S Morrienn 02 10 2112 Incubators Local Service Center 2 Ace Electric Lab Systems 1 Ace Lab Systems www acelabsystems com Customize Links 44 Google 42 STLOC SMET Divisi Other bookmarks STLCC College Intra St Louis Community Center For Plant and Ace Lab Systems 1550 5 Kingshighway St Louis MO 53110 Tel 314 771 7272 Fax 314 771 5855 Contact Protecting pour laboratory environment Thermo THE BAKER COMPANY SCIENTIFIC Ace Lab Systems is a complete laboratory equipment repair center service nearly all makes and models of Centrifuges Spectrophotometers Biological Safety Cabinets LS amp Gamma Counters
3. filter and two silicone plugs 760199 Door Kit and Right Hand Door Swing Independent Inner Glass Door Kit eight glass doors with latches mounts inside heated inner glass door is removable and can be autoclaved Fig 02 190650 Right Hand Door Swing factory installed at time of order 130888 UO Accessories Built In Gas Guard monitors and automatically switches fram one cylinder to the other when the supply 15 exhausted factory installed 1900088 Wall Clamptora 02 Bottle includes cylinder holder with web strap 950151 8 Two Stage COs Gas Regulator with barbed connection and shut off Fig 03 3850 0 Oe Fyrite Analyzer Kit 0 2096 15502 1 Roller Dolly Roller Dolly heavy duty powder coated steel base with dual wheel swivel locking casters and leveling feet supports ane or two stacked 1900063 Incubators raises unit 3 0 bcm off the floor Fig Data Outputs select one factory installed 9 hure pdf 85 interface 1900085 4 20 milliamp 0 5 analog 1V analog 190544 Page 44 eee Incubators CO2 Tank Pressure vs Volume 2900 L s Pressure vs Fill and Temperature 100 Fill oz cu in 60 agr 200 Minimum Blowout 2200 psi Liquid apor Phase 120 F psi M VEMM NIMES EET NR IE 2000 969 855 110 30 70 f 50 F 747 552
4. Digital PID Microprocessor Controller Patented CLS Custom Logical Safety System exceeding DIN Class 2 Chamber Volume 250L Temperature Control Range Amb 5 0 60 0 C Type 4 0 60 0C Auto Tuning and Bias Function Internal Sensor Calibration 99h 59m Timer with Time Delay ON OFF Door amp Over Temperature Alarms Shaking amplitude 30 40 50 60 and 70mm 30mm standard RS 232 with FREE LabTracer Software 10 to 300 rom Shaking Speed with Opto Electronic Feedback 999h 59m Shaking Timer Programmable motion Clockwise Anti Clockwise and Pause Dimenstions 44 6x31 9x41 1 inches Weight 379 lbs Optional Flask Clamp Universal Platform Spring Wire Rack Separate Funnel Clamp Test Tube Rack Rubber Mat 9 Link to Jeiotech 15 971 User Manual Link to Jeiotech 15 971 Engineering data Link to Jeiotech 15 971 Calibration Supplement STLCC CPLS Morrison 2 19 2013 Page 9 cubator Shaker Control Panel BRDG Present SET A 29 0 ctive not indicator RPM H 7 i i P if Shake On off HEAT special Sy TIL motion is Wait off Timer oor Onn Alarm SET TEMP SET TIMER ENTER SET SHAKE 1 START STOP TEMP ONLY SHAKER ON OFF Toggle MASTER ON OFF SET MAX GREEN BUTTON 02 21 2904 TEMP LIMIT STLCC CPLS Morrison 2 19 2013 Page 10 Incubator Shaker Basic Controls Set Temp
5. 260 Ibs 117 9 kg 315 Ibs 142 9 kg 4 5 Mee 2203 G 2 Windows Microsoft Excel Forma Steri C St Louis Com E http www ica Microsoft 12 PM Display Humidity Display readable in 155 increments includes low RH programmable alam alerts you of need to add water to humidity 1900091 factory installed Shelving Ductwork and Humidity Pans Stainiess Steal Campansnts stainless Steel shelf and Channels 190554 Stainless Steel Humidity 2 Incu bator Stainless Steel Ductwork Kit includes side ducts and shelf channels 190870 Sterilizer Salid Companents Model 3970 Copper Interior Components Kit includes sid e ducts shelf channels four shelves and humidity pan 1900095 factory Installed at time of order PartNos Copper Perforated Shelf with Channels 19087 Copper Humidity Pan Fig 01 237 EU Filters and Filter Kits Replacement HEPA Filter 04 FeO value Pack four filters ZB US 10 Disposable Polypropylene In Line Filters FB TU HEPA Filter Replacement Kit includes HEPA in line and access port filters 1900082 Replacement HEPA WOC Filter 0200 HEPA WOC Filter Replacement includes in line and access port filters 1900094 HEPA VOC Filtration System kit converts HERA Filter Airflow System to Filtration System includes
6. door is open gt 1min press START to resume heating look for blinking heat light TEMP To set TIMER To set ENTER To temperature timer mode accept values If Door is open gt 1min Alarm will sound and you must press START to resume previous settings temperatures Mode ls Chamber Volume SO cu ft 833 5 liters Convection Technique Mechanical Temperature Control Hydraulic Temperature Range Ambient 5 C to 70 Unitormaty 41 37 Sensit ity 41271 Recovery Dime as 14 minutes D mensions Lx W x Hi Chanter 23 8 SOK Al 6x T22x 1829 each 23 29 5 Im 50 0 724 cm Extenor 5x35 74 38 914 x 2248 cm Elect ical Specs 50 60 Hr 1201 14 50 Watts 12 1 Amps ETU 4452 She hes supplied Shippmg Data rrenstons 42 x 95 x 40 in 105 7 x 241 3 x 101 5c mi Weight 310 7 kgl B cu ft 12 52 meters Catalog Humber LH Hinge Solid Door 2371 LH Hinge 81855 Door 3973 Add 2 5 inches p ofunittor door Unit 5 shipped on rts back n a wooden Incubator 30cuft Precision Sci Model 30M Property of St Louis Community College CPLS Donated by Gallus The temperature range is ambient 5C to 65C Precision Mechanical Convection Incubator Model 30M Glass S N 699050714 Biological Indicator testing 55C 60C 10
7. 12 years Unit has been maintained validated and calibrated on scheduled basis Production speeds N A 120V 1450 W 12 1 Amps Jean Stuckey QC Microbiology Manager Gallus BioPharmaceuticals LLC 314 733 3107 office 618 407 3880 cell 4766 LaGuardia Drive St Louis MO 63134 3117 Precision 30M Incubator Page 30 Incubator Precision 30cuft Controls Donated Gallus Set Over temp range Set Temperature adjustment knob screw adjustment knob screw Over temp range light 2 Qu uer DC AE ve Ce lighted when heat indicator on at limit elements are on active Interior light on will illuminate with door open Power connected indicator light Heating Master Switch Heat Wattage Knob 350 700 1400 Set on for normal use Use Higher settings to reach set point faster emporary Ue heat range screws then set to lower wattage to maintain GENERAL OPERATIONS temperature set screw knob clockwise to increase temperature setting Set wattage to highest value 1400 t o reach set point faster Set limit or range for allowance above set point temperature Monitor temperature gage and adjust temperature screw knob until desired temperature is reached oet wattage to a lower value to maintain temperature monitor for other changes STLCC CPLS Morrison 2 19 2013 Incubator LabLine Imperial IIl FV 1 LabLine Imper
8. 1800 Copyright 2005 Artisan Scientific Corporation Internal Dimensions cm 33 7 W x 18 1 Dx 16 6 H External Dimensions cm 46 2 W x 22 4 D x 26 7 H Weight 9 7 kg UV Bulbs 8 Watts Each Power Delivered 3000 uwatts cm The Stratalinker amp UV crosslinker is designed to crosslink DNA or RNA to nylon nitrocellulose or nylon reinforced nitrocellulose membranes The process takes only 25 50 seconds in contrast to the traditional method of baking filters at 80 C for 2 hours Additionally crosslinking has been shown to significantly increase hybridization signals when compared to oven baking Each UV crosslinker is equipped with an internal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs The crosslinker may be used for Northern Southern dot or slot blot analysis 1 3 colony or plaque screening nicking of DNA in agarose gels prior to blotting 4 dimer formation to perform partial digests for rapid restriction mapping 5 UV sensitivity testing for host strain verification 6 UV irradiation of PCR samples Three independent modes of operation Time Energy or Autocross Link to Stratagene Stratalinker Manual pdf STLCC CPLS Morrison 2 19 2013 Page 48 UV Crosslinking Modes of Operation Stratalinker 1800 Selecting the Mode of Operation The mode of operation selected depends on the specific results desired Below is a brief explanation
9. Meets or exceeds all safety performance and sanitation standards set for Commercial Food Service Microwave Ovens by HHS FCC and NSF UL listed Capacity 19 8 L 0 7 cu ft Electrical 120V 60 2 7OOW Interior Dimensions 32 1W x 33 7D x 20H cm 125 8 x 131 4 x 77 8 Shipping Weight 15 kg 33 Ibs Page 36 Incubator Plant Percival Upright Percival Adv Intellus Environment Control Model CU36L4 A SN 9012 01 061 110V 10amp Refr 134A 150z Temp Range All lights on 10 44 0 7 C Interior Space Volume 29 6 ft3 0 84 m3 Total Shelving Floor Area 22 ft2 2 4 m2 Maximum Growing Height 7 7 8 in 20 cm Exterior Dimensions inches cm Height 77 195 6 Width 33 5 85 1 Depth 33 9 86 1 Light Intensity 6 from lamps unless otherwise noted 140 umol m2 s Link to User Manual pdf Link to Installation Maintenance Manual Placea pan under the center of the unit to capture any condensation dump periodically If individual lights in bay not check to ensure that the lamp metal end probes extend into the plastic end caps at both ends and make sure the end caps are securely in place If this is the case and the lamp does not light it is probably burned out bad Swap a suspected burned out lamp with another rack spot that is working to validate this If an entire bay of lamps are out one or more lamps may be improperly secured in the plastic end caps Remove each and
10. Set RPMs Current RPM Display 0 if OFF 5 9 1 SV PV MU PAUSE V m raat 25 25 gt O T Lab 3 Companion SET and HOLD TEMP SET and HOLD SHAKER RPMs CHANGE RPMs TURN OFF RPMs Turn on device with GREEN button Press SHAKE button until is shown in PV LED Use Up Down arrows to change value 4 Press ENTER to accept value for RMP set point Press START to begin motion immediately Press SHAKER I O ONCE to start motion Observe RMPs climb and rotating symbol to SV is reached 1 Press TEMP once or until SV blinks PV LED may cycle thru Sv1 Sv2 SV3 Uni 0 0 until blink mode is reached Use Up Down arrows to change value Press ENTER and wait until SV blinking stops Press START button to activate temp control Observe TEMP LED on To Change RPM value press SHAKE until is displayed HEAT LED blinks until temp SV is reached up down arrows to new SV then press ENTER ee Three frequently used temps can be saved in To Turn off Shaker press SHAKER once only the Sv1 Sv2 Sv3 modes ENTER to activate Observe drop in RPMs motion symbol at bottom Press TEMP until Uni is displayed in PV LED d cape Pe to change units from and finally stopped will display 0 To Synchronize TEMP with RPM settings you must cycle SHAKE button to Snch and set To Synchronize TEMP
11. can be calibrated by pressing the Up Vown pads until the display reads the correct value Allow the Incubator temperature to stabilize again and recalibrate if necessary Set Overtemperature Thermostat As mentioned in step 5 2 the Overtemperature Thermostat should be initially set ta its maximum position to allow the unit to stabilize Once the incubator is stabile at the desired set point turn the Thermostat counterclockwise until the OYERE TEMP light turns on turn the Thermostat clockwise just until the light turns off Then turn the Thermostat clockwise two of the smallest divisions an its scale past the point where the light went out This will set the Cyertemperature Thermostat at approximately 17 C above Main temperature set point age 23 Incubator Shel lab 40cuft Reach in CO2 Regulated SILCC CPLS5 Morrison 2 19 2013 42 w x 34 deep x 88 high Sheldon Model 1927 SN 1200300 Heat control Tested at Oakland Warehouse 6 8 11 Moved to BRDG by July 2011 CO2 Loss rate 196 per hour tested 9 13 11 or about 24 cu ft per day without door openings and at Zero input pressure gas off Page 24 Incubator Temp and CO2 Control Panel Shel lab 40cuft Set for max allowed AN SET TEMPERATURE HIGH 3 7 0 0 LOW A CR AV POWER UP DOW 3 HEATING ACTIVATEL Use up down to set Temp degrees C SILCC CPLS Morrison 2
12. make sure the lamp metal ends extend into the end cap and re secure end caps STLCC CPLS Morrison 2 19 2013 Page 37 On Off Toggle Switch side Control Panel see other slides Incubator CO2 Steri Cycle Thermo Electron CO2 Connection in Rear STLCC CPLS Morrison 2 19 2013 Sterilizer Cycle On Operating Button The Model 370 direct heat Steri Cycle CO2 incubator combines the best of both worlds a unique in chamber HEPA air filtration system providing continuous protection against unwanted airborne contaminants and an on demand high temperature sterilization cycle to simplify your routine cleaning practices Providing precise CO2 control with choice of TC thermal conductivity excellent temperature uniformity and recovery characteristics Specifications Temperature Range 5C above ambient to 50C Temperature Uniformity 0 3 37C Humidity System 3 2 quart 3 0 liter pan Humidity Range Ambient to 95 37C Interior Volume 6 5 cu ft 184 1 liters Interior W x H x F B 21 8 x 26 8 x 20 0 54 1cm x 68 1cm x 50 8 cm Exterior W x H x F B 26 3 x 39 5 x 25 0 66 3cm x 100 3cm x 63 5cm Ship Weight 315 Ibs 142 9 kg Page 38 Incubator CO2 Control Panel 22 Mode Button Cycles are Run Set Calibrate Configure TITDTGITUIT Temp ous RH optional System Configuration Configure audible on off access code HEPA filter
13. to Snch and set mode to YES then ENTER 12 Incubator Floor Shaker Start Issues SS Page 13 STLCC CPLS Morrison 2 19 2013 Incubator Shaker Temp Calibration Should be run at initial setup and after any significant relocation or changes in ambient environment ES ax L 1 L 1 step 1 Press the power H switch to turn the unit on if it is not already on Allow the controller to stabilize and the main display to appear as shown in Figure 3 21 Step 2 Place a scientific digital thermometer into the chamber close the door and allow the thermometer to stabilize Make sure the thermometer display is readable through the incubated shaker s observation window if it does not have a remote display Step 3 Compare the thermometer reading to that shown on the PV display Step 4 there is a difference press the TEMP key six 6 times The PV display will show the revised value and the SV display will read BIAS as shown below Step 5 Press the UP 4 key or DOWN V key to reflect the difference between the present value and the thermometer reading the BIAS value display m Step Press the ENTER m key to complete the calibration adjustment step 7 Press the ENTER A key to return to the main display 11 9 20 Unknown Zone 14 Incubator Shaker Auto tune Function Should be run at initial s
14. with RPM settings you must mode to YES then ENTER cycle SHAKE button to Snch and set mode to YES then ENTER D Page 11 Incubator Shaker Detailed SHAKE Operations W ON PAUSE W OFF 7 B8000 o6 SET TIMER for SHAKE Operations Use RPM set mode previous slide to set RPMs for Timed Operations NOTE Total time for run HOUR setting MINUTES SECONDS settings 1 For HOUR SETTING Press SHAKE button until runH is displayed Use Up Down arrows to change value Shake LED blinks Press ENTER to accept value press SHAKE to start timer 2 For MINUTES and SECONDS SETTING Press SHAKE button until runS is displayed Use Up Down arrows to change value Press ENTER to accept value press SHAKE to start timer 3 For FORWARD Clockwise Time Shaking Press SHAKE button until For S is displayed Use Up Down arrows to change value Press ENTER to accept value press SHAKE to start timer 4 For BACKWARD Counter clockwise Shaking Press SHAKE button until bAC S is displayed Use Up Down arrows to change value Press ENTER to accept value press SHAKE to start timer 5 For PAUSE in shaking time Press SHAKE button until Pau S is displayed Use Up Down arrows to change value Press ENTER to accept value press SHAKE to start timer 6 To Synchronize TEMP with RPM settings you must cycle SHAKE button
15. 16 Document Commodity Total 992 16 Distribute FOAPAL of 1 Remaining Commodity Amount m o gt Index Fund Orgn DE 210200 z Actv m HW STLCC_CPLS Morrison 2 19 2013 gt 0 00 USD oe Discount Additional 8 FOAPAL Line Total Document Accounting Total a 992 16 26 Incubator Bench 2 cft VWR Model VWR E1510 2cuft from Sheldon Manf Weight 71 00 Ibs 2 Place thermometer and Incubator Interior Dimensions WxDxH in cm 17 x 12 x 17 in Interior Dimensions WxDxH in cm 21 5 x 18 x 25 5 in samples on shelves Temperature Range 5 C above ambient to 70 C www shellab com 5 Monitor Actual vs Desired Temperature Adjust setting as needed Temp C vs Setting Temp Centigrade 1 On Off 3 Set Temp Control 4 Per Chart for Approx Incubator Temp Setting Temperature C STLCC_CPLS Morrison 2 19 2013 Page 27 Incubator Benchtop or Stacked FV LabCompanion Lab Companion Air Jacketed Incubators Jeio Tech Inc China CLS Control System and PID microprocessor controller with enhanced safety features IB 250 Incubator Temperature safety exceeds DIN Class II controller Auto Tuning function amp Bias function internal built in re calibration Timer Time Delay ON OFF standard 99hr 59min Selectable measuring temperature
16. 19 2013 over set temp value SET OVERTEMPERATURE OVERTEMPERATURE Use up down to set Desired CO2 level 96 Set Regulator on Supply tank to about 12 PSI Max Page 25 Incubators CO2 Tank order Airgas continuing rom 8 Taylor Angela M Rawena BelliAirgas com 8 Staerk Becky E Morrison Robert G Green Cindy E Norris Richard J Boedeker Elizabeth D RE RESUME coz ORDERS Airgas for Carbon Dioxide Every Two Weeks ee Requisition R1203786 Type corrected on date Opps Order Date 20 OCT 2011 25 Transac Delivery Date 28 0 2011 2 2 Commodity Total Sent Thursday October 25 2012 8 55 AM 992 1 16 Accounti Rowena Bell Airgas com Cc Staerk Becky K Morrison Robert G Green Cindy Norris Richard J Boedeker Elizabeth D Taylor Angela M Subject RESUME CO2 ORDERS Airgas for Carbon Dioxide Every Two Weeks Hello Rowena equestor Delivery Information Vendor Information Please add the following to our order due tomorrow 10 26 12 U M Tax Grou r Product CO2 2 tanks CDUSP50 zx 1 cA Date Needed 10 26 2012 Commodity Description Purchase Order P1203333 Frequency gt Item Text Add Commodity Every two weeks until 12 21 12 that s the Mayan calendar end date isn t it Commodity Line Total 992
17. 2096 15 PSIG 1 0 bar T C or IH 0 1 Userprogrammable high low Ambient to 85 4 37 98 66 3 7 qt 3 0 liters standard In 195 increments 1 3 3 3cm with removable silicone plug with filter 1 4 hose barbed 233 BTUH 86 Watts Shelves Dimensions Construction ourface Area Max per Chamber standard Maximum Construction Interior Volume Interior Exterior Outer Door Gasket Inner Door Gasket Electrical 370 380 3 1 381 Circuit Breaker Power Switch Convenience Receptacle Plug Alarm Contacts Data Outputs opt Dimensions Exterior Interior Weight Shipping Matar 18 5 x 18 5 47 x 47 stainless steel perforated 2 4 sq ft 0 2 sq m 25 0 ft 3 3 sq 4 15 6 5 cu ft 184 1 liters Type 304 polished stainless steel 18 gauge cold rolled steel powder coated Four sided molded magnetic vinyl Hemovable cleanable feather edged silicone 1 15V 50 50 Hz 9 5 FLA Operating range 90 125 230V 50 50 4 4 FLA Operating range 180 250 12 Amps Pole 75 Watts maximum matches cabinet voltage 115V NEMA 5 15P Plug 230V CEE 7 7 Plug Power interruption deviation of temp HH customer connections through jack on back of unit H5 485 0 1 0 5 4 20 milliamp select 25 3 W x 39 5 x 75 0 F B 8 x 100 3cm x 21 3 W x 25 8 H x 20 0 F BIbA 1cem x 58 x 50
18. 9 9 hrs For safe operation both the C 25 and C 25KC are designed with a safety switch that automatically stops the shaker mechanism when the lid is lifted In addition the C 25 and C 25KC are equipped with visual and audible alarms that alert the user to the following conditions end of a timed run Deviations of shaking speed Deviations of temperature setpoint Power failure Lid open A wide variety of platforms can be used with the C 25 or C 25KC Product Family C 25 C25 C 25 C 25KC C25KC C 25KC STLCC CPLS Morrison 2 19 2013 Page 20 Incubator Shaker Floor FV C 25 Specifications Speed Control Accuracy Indication 3 Digit LED in 1 rpm increments Stroke 1T 2 54 cm Temperature C above ambient temperature to 60 C Control Accuracy 0 25 Indication 3 Digit LED in 0 17 increments Ambient Operating 5 35 C 20 to 90 relative humidity non condensing Environment 0 1 hr to 99 9 hrs Shuts off at end of period Visible and audible waming indication when speed deviates Alarms more than 5 rpm and temperature more than 1 C from points and when timer has expired Timer Automatic restart after power Is restored setpoints and Automatic Restart operating status are retained in memory during power interruption 220 240V AC 50 60 Hz 1320 VA 30 X 18 76 X 46 cm Stainless Steel 443 WX29 D X334 H 113 X 74 X 85 cm Chamber Dimensions 420 Ibs 191 Net 450
19. H D 18x16x12 inch W H D 20x25x14 cm Weight 78lbs Temperature Dial Calibration Calibrating the actual chamber temperature to the temperature Do not adjust control to operate above printed on the knob dial should only be done if the chamber the oven s stated maximum operating temperature as measured by a known accurate device is off by temperature more than 15 F 8 C from any indicated dial setting The most accurate calibration possible for any analog type control is at a single temperature setting Therefore calibrate the control at the most frequently used temperature To calibrate Set dial temperature to a frequently used temperature then remove knob allen screw Be careful not to move the position of the shaft when removing the knob Allow oven to achieve a stable temperature Remove compression pin newer models only to access the trim screw recessed in shaf center Using a small blade driver turn the trim screw clockwise CW to adjust temperature down and CCW to adjust the temperature upward Make only small 1 16th adjustments at a time Allow the temperature to settle before making any subsequent adjustments NOTE Use pliers to keep outer shaft from turning when making trim screw adjustments STLCC CPLS Morrison 2 19 20 15 Incubator Refrigerated Shel Lab Bench Floor Model LI5 SN 09009810 p Microprocessor Control Temperature Control LED Display of Setpoint and Chamber Temper
20. Ibs 204 kg Gross Ambient temperature is defined as the temperature within one meter of the shaker mes LLL 2 InFermek 1 ipid 937 RE 7 2013 8 note gadget txt Mote 1 Windows Explorer Google Image Result bet Incubator Shakers 5OP Bay Incubator Floor Upright Reach in Sheldon Manf 1126 28 cu ft Reach In Incubator 120V Sheldon Mant Reach In The Model 28 incubator provides extra large capacities while minimizing the amount of floor space used This large space is kept uniform by intergrating a highly responsive mircoprocessor with the appropriatly sized heating element A totally independent secondary temperature controller offers the added security of over temperature protection Our unique forced air circulation system creates a one pass circulation pattern that provides both exceptional temperature uniformity and rapid heat recovery The chamber floors are ruggealy reinforced to support roller apparatus or shakers The HI28 is supplied with six 6 study shelves that will not sag or bend under heavy loads Microprocessor Control 5C to 70C Power 120V 8 5A 28 cuft internal Viewing Window Accomodates Roller Bottle Apparatus Power Coated White Interior Supplied with 6 Shelves 12 Month Warranty Hotlink to Shel Lab RI28 Incubator User Manual pdf STLCC CPLS Morrison 2 19 2013 Page 22 5 5 5 6 5 7 Incubator Shel
21. Incubators Ovens Sterilizers Refrigerators Freezers Ultra Calds Liquid Nitrogen Storage Containers Vacuum Pumps ThermalCyclers Shakers Power Supplies Water Baths Gel Dryers Refrigerated Vapor Traps Ice Machines Washers and many more Ace Lab Systems is to keeping alder equipment in service while keeping costs to a minimum On select equipment Ace provides component level repair on circuit boards where a new circuit board could costthousands have a B week lead time ar GBSOLETELI Many parts available for older equipment we service yet deemed obsolete Whether you are a Doctor using the equipment a Bia Med technician needing help a lab tech you can turn ta Ace for expert service Ace also sells used equipment at great prices Take look at same of aur used equipment now Soon you will be able ta browse through aur entire inventory online Ace can provide your organization with Total Service Contracts Preventative Maintenance Contracts ar a contract tailored to your needs Please email us the make model and serial ofthe unit a description af the services needed your location and we will promptly respond to your request Thank ou Far Service Questions ar Comments Email us Incubator OVEN 2 Cu ft Gravity Convection Quincy Labs via MIDSCI Model 30GC SN G3 008946 Capacity 2 0 Max Temp 450F 232C Min Ambient 25F or 37C specs but tested at 44C Watts 1200 Amps 10 43 W
22. Lab 28 cuft Main Controls Set Main Temperature Controller Enter desired set point temperature enter set paint made on the controller press either the Up ar Down arrow pad one time The digital display start ta blink going fram bright to dim V hile blinking the digital display is showing the set paint change the set point use the Up and Down arrow pads lf the arrow pads are nat pressed for five 5 seconds the display will stop blinking and will read the chamber temperature Mote that the Overtemperature Thermostat should be turned to its maximum position clockwise until the unit has stabilized at desired set point temperature Allow the incubator at least 24 hours 10 stabilize Calibration 15 recommended that calibration is done ance the unit is installed its working environment and has been stabile at set point for several hours Place a certified reference thermometer in the chamber where it can be easily viewed through the window Ge certain the thermometer 15 not touching any shelving Allow the temperature to stabilize again until the thermometer reads a constant value for one hour Compare the digital display with the reference thermometer If there is an Unacceptable difference put the display into calibration mode by pressing both the Up and arrow pads at the same time for five 5 seconds until the outside decimal points begin to flash While the decimal points are flashing the display
23. any systems are operational Main Temperature Control his control 15 marked SET TEMPERATURE and consists of the digital display and LP DOVVR arrow pads for inputting set point temperatures and calibration Heating Lamp This plot lamp 15 marked TEMPERATURE ACTIVATED and is ON when the unit 15 heating up to set point and is blinking when controlling temperature at set point Overtemperature Thermostat his control 15 marked SET OVERTEMPERATURE and 15 completely independent of the Main controller acts as an override in the event that the Main control falls In the GN position Overtemperature Light his pilot lamp is marked OVERTEMPERATURE ACTIVATED andis on when the thermostat has been activated Under normal operating conditions this pilot light should never be Circuit Breaker Non CE units Adjacent to power cord the circuit breaker 15 an added measure of protection against power source variations that if tripped must be reset by pushing in the button once the reason for interruption has been cleared Fuse units only Located at the back of the unit within the power inlet the fuse is an added measure of protection against power source variations that If blown must be replaced once the reason for the interruption has been cleared Note that the unit will not power up unless the fuse Is in place Incubator Refrigerated Control Panel Page 6 4 1 4 2 4 3 4 4 4 5 4 6 4 Check po
24. ature High and Low Limit Thermostats Capacity 3 2 cu ft Interior Electrical Outlet Fan Assisted Forced Air Circulation Hermetically Sealed Compressor Interior Dimensions WxDxH in cm 16 x 14 x 25 41 x 36 x 64 Exterior Dimensions WxDxH in cm 24 x 22 x 33 61 x 56 x 84 Temperature Range 0 C 45 at 21 C ambient Temperature Uniformity 0 5 C at 20 C Electrical Specifications Volts 120V Hertz 50 60Hz Watts 400W Amps Capacity of Standard B O D Bottles 62 Shelving 2 Supplied Interior Outlet 110V 1 Hot link to Sheldon Incubator User Manual pdf STLCC CPLS Morrison 2 19 2013 Page 4 Incubator Low Temp ShelLab Basic Operations Set Main Temperature Press either the Up or Down arrow ONE TIME While the display is blinking continue to press the up or down arrows to reach the desired set point Temporarily set the Over temperature dial to the maximum position clockwise until the desired set point is reach After the set point has stabilized turn the Over temperature dial back counter clockwise until the light goes out then clockwise again just past this mark STLCC CPLS Morrison 2 19 2013 Page 5 ST 8 8 8 3 1 3 2 3 3 3 4 3 5 3 6 3 7 Power Switch main power ron otf switch controls all power to the unit and must be in the HON position before
25. be rack funnel clamp funnel clamp funnel clamp funnel clamp microplate rack tower microplate rack 4 flat microplate rack 3 flat STLCC CPLS Morrison 2 19 2013microplate rack single Capacities MIDSCI LabCompanion http shop midsci com productdetail M50 AAH23336U Size 50 100 250 300ml 500ml 1000ml 2000ml 2800ml 4000ml 6000ml n a 250ml 500ml 1000ml 2000ml n a n a n a n a IS 971 R tj O1 c Page 16 Incubator Shaker Platform Options htto www gmi inc com Genlab Jeiotech 20971 20Series 20Incubator htm STLCC_CPLS Morrison 2 19 2013 Page 17 Incubators Floor Shaker Parts List CODE NUMBER PART NAME DESCRIPTION QUANTITY heel Rotation Pin Set and Shaking System Belt Pulley 1 Set J tvpe 4 thread 40 Limo Sv BLOC EE A142 T FAAS113 CAS 10 CHE 4101 CHE44 O0 8128 23 mes 5 Page 18 DET STLCC_CPLS Morrison 2 19 2013 Incubator Shaker Floor Belt Jeiotech Lab Companion 5t Lows Community College at BRD Park Center for Plant and Life Sciences 1005 Warson Ed 123 Creve Coeur 63132 Contact information Transportation Method Ship Date Currence y of Payment Validity 2 e a TECH Quotation 1 4 Gill Street Phone 781 376 0700 Fax 781 376 0704 Email customerservi
26. change raminder remote alarm contacts tracking low temp and high and low alarms ad the following options automatic tank Up Down Used 727 15 185 interiace and display to set values selectable Run Class 100 timing reminder appears after door is closed for five Enter when minutes message changes to describe done alarm conditions Audible Visual Alarm o Setpoint Set temp overtemp COs Heater On Indicator Temperature Display Alarm Silence 15012 Enter When an option and value has been Left Right set moves through options in each mode Optional Built In Gas Guard System G 0 Inject Indicator Mode Select m m m Unknown zone Z5 7 LJ 9 D 0 J 4 Done Incubator CO2 Activating Sterilizer Cycle Recommended at Semester Breaks and or before new Projects Turn on device on left side Use Mode button to select RUN Press and Hold HEAT button 5 seconds to activate 4 Follow messages remove HEPA remove Water 5 Press ENTER when ready to start Sterilization Cycle 6 Cycle runs through four phases The Cycle Starts with the Press of a Button is rampi total about 5 hours During the heat sterilization process the Micro processor control monitoring system s message center guides you through the cycle w
27. eiatech com 113 12 02 5t Lows Community College at Park Center for Plant and Life Sciences 1005 Warson Ed Room 123 Creve Coeur 63132 Belt for 5 27 1 SK 71 34 84 34 84 t SHIPPING CHARGES ARE NOTINCLUDED t PLEASE MOTE PART WILL BE SHIPPED WITHIN 1 2 DAYS AFTER WE RECEIVE PAYMENT Out of state sale exempt from sales tax D Drs 0 00 19 8 5 wy Incubator Shaker Floor NewBrunswick FV New Brunswick Scientific C25 Incubated Floor Model Shaker Part Number M1246 0000 44 W x 25 D x 34 Ti The C 25 Classic Incubator and C 25KC Classic Refrigerated Incubator Shakers are large capacity shakers utilizing an eccentric counter balanced drive to provide horizontal plane rotary motion in a 1 2 54 cm circular orbit A ELS Proportional Integral Pl Microprocessor controller with 3 instantaneous digital feedback controls the speed over a range 3 of 50 400 rpm The C 25 provides temperature control over a 7 range of 7 C above ambient to 60 C and the 25 provides m temperature control over a range of 15 C below ambient minimum 4 C to 60 C Ambient temperature is defined as the temperature within one meter of the shaker The shakers may be operated either continuously or in a timed mode via a programmable timer for shaking periods of 0 1 hr to 9
28. etup and after any significant change in operating system environment or extreme run conditions During auto tuning the controller heats to 75 of the set point temperature where it oscillates above and below the set point as many as three 3 times before loading the new tuning parameters After the tuning parameters are loaded it heats to the set point temperature Auto tuning is dependent on the operating conditions and can take up to 45 minutes It is best done before any experiment is run S Step 1 Press the Temp key one 1 time The setting in the SV display will flash Display Flashes Step 2 Press the UP key or DOWN v key to set the desired temperature Step 3 Press the ENTER key to finish setting the temperature Step 4 Press and hold down the A T key for one 1 second or until the A T LED illuminates and the PV TEMP and SV displays change to the following HEAT Flashes during auto tuning W ON W OFF Step 5 Press START STOP V key The TEMP DOOR HEAT LEDs will illuminate and the A T LED flashes to indicate that the controller is tuning itself When O T the A T LED light stops flashing the controller is 850x11 00im 0 41 pre 1 STLCC CPLS Morrison 2 19 2013 Page 15 Incubator Shaker Racks Clamps Attachment flask clamp flask clamp flask clamp flask clamp flask clamp flask clamp flask clamp flask clamp flask clamp flask clamp test tu
29. ial Ill 120V 41 W x 28 Dx 35 Control dial Thermostat Control dial STLCC CPLS Morrison 2 19 2013 Page 32 Incubator Chicago Surgical LabLine FV Model Cat 600 Cat 600 SN 0 69 gt 120V 500Watts 41 W x 26 Dx 40 H 4 17 Tm On Off Analog Dial Temp Control 0 9 STLCC CPLS Morrison 2 19 2013 Page 33 Incubator Fisher Scientific lsotemp FV 4 Woods 20 C Freezer ref STLCC_CPLS Morrison 2 19 2013 Fisher Scientific Isotemp Incubator STLCC 001804 25 w x 25 D x 45 H Fisher Scientific Isotemp Incubator STLCC 001805 25 w x 25 D x 45 Set at 36 8C Page 34 Incubator Microwave Oven Countertop Turntable FV GE 7 cu ft Typical PN DE68 40289B STLCC CPLS Morrison 2 19 2013 Page 35 Incubator Microwave Benchtop VWR Argos Compact Microwave Oven Argos Technologies STLCC_CPLS Morrison 2 19 2013 sell X The compact design of this 19 8L capacity oven makes it ideal for the crowded lab as it occupies only minimal counter top area The control panel is easy to read and use The Minute Plus feature sets the oven at high power with a single touch An internal turntable rotates during operation for even heat distribution Argos 111 071 Note Unit is not explosion proof and is not intended for use in acid digestion applications
30. imit Alarm RH option RH IS LOW 30 min Programmable Tank 1 is low switch to Tank 2 Gas Guard only TANKI LOW No Tank 2 is low switch to Tank 1 Bas Guard only TANK2 LOW mn No All alarm delays and ringback times are 240 seconds When multiple alarm conditions occur active messages are displayed in the message center one at a time updating at 5 second intervals Pressing Silence during multiple alarms causes all active alarms to be silenced and to ring back in 15 minutes The TEMP I5 LOW alarm is disabled when the Temp set point is 10 C The alarms are disabled when the CO set point is 0 0 Page 42 e 2 Incubator Sterilizer Model 370 Specifications Temperature Control Range Uniformity Tracking Alarm vertemperature Sensor Funetion Temperature Safety Sensor Controller Sterilization Cycle Cycle Temperature Cycle Length Contral Range Inlet Pressure Sensor Headability amp Setability Tracking Alarm Humidity HH Humidity Pan Display Fittings Access Port 0 Inlet Unit Heat Load 115V 230V 30 17C 5 C above ambient to 50 C 122F 30 37 C 37 98 6 LIser programmable low Precision thermistor Shuts off heat Independent thermastat Independent analog electronic Precision thermistor 140 C Z84F Under 12 hours Better than 20 1
31. ing both the and lawn arrow pads at the same time until the two outside decimal points begin flash While the decimal points are flashing the display can be calibrated by pressing the Up or Down arrow pads until the display reads the correct value Allow the incubator temperature to stabilize again and recalibrate t necessary Set Overtemperature Thermostat As mentioned previously the Lvertemperature Thermostat should be initially set to ts maximum position clackwise to allow the unit stabilize Once the incubator is stabile at the desired set point turn the Overtemperature Thermostat counterclockwise until the OVERTEMPERATURE ACTIVATED light turns on turn the Thermostat clockwise just until the light turns off Then turn the Thermostat clockwise 1 16 of an inch past the point where the light went out This will set the Cveremperature Thermostat at approximately 17 C above main Temperature set paint Accessory Outlet This unt has been supplied with 1 amp accessory outlet Inside the chamber to provide power for equipment such as magnetic stirrers rockers etc Be certain the apparatus draws 1 amp less The power switch on the front panel controls power to the outlet Mate that the apparatus may provide additional heat that could affect the temperature range of this incubator It is recommended that testing be done with the incubator and any accessory equipment to insure that the desired operating conditions ca
32. ith start up and cycle status messages The three sterilization cycle phases are heat sterilizing hold and cool http www thermo com eThermo CMA PDFs Product productPDF 7382 pdf Microsoft Internet Explorer ni File Edit GoTo Favorites Help Q O 3 292 GENI MS NL Address http thermo comfeThermo CMA PDFs Product productPDF_ 7382 pdf sB A Sterilizing Phase Chamber has reached the Cool Phase Incubator is cool to normal operating sterilization temperature and all microbial life is temperature you are then destroyed to replace the HEPA and sensor if applicable H 2 Page 40 d start BBE x 3 1 Te 5 Microsoft Excel amp Forma Steri a St Louis http www O Microsoft Po 12 09 Incubator CO2 ThermaForma 370 HEPA and In line Filter e Replacing the Filter Open the outer and inner glass door Remove the old filter by twisting and pulling downward Locate a replacement filter and write date of replacement on it Install the replacement see info at right filter and make sure it is pushed completely against the mounting cylinder ring e Setting the HEPA filter reminder Press the Mode key until the Config i
33. n be met Incubator Refrigerated Sheldon Operations Page 7 9194533 Incubator 3 7cft VWR Gravity Convection 48 5W 9 53 6 19 16 x 211 3 64 5W x 57 70 x 84 1H cm 2519 45 x 2279 35 x 331 3 120V 60 2 320W 88 kg 191 Ibs 69 9W x 65D 100 1H cm 27 5 x 259 x 3913 32 105 L 3 7 cu ft 14004 622 Each 1 928 56 STLCC CPLS Morrison 2 19 2013 Seven Segment LED Digital Display Two Year Parts and Labor Warranty VWR Gravity Convection Incubators are ideal for safe incubation with reduced air changes providing a stable environment while minimizing the potential of drying out samples The digital advanced adaptive microprocessor control system provides superior temperature accuracy The PT100 temperature sensor provides the best overall advantages in repeatability and Stability over extended time periods All incubator units feature a 3 3cm 1 3 stainless steel ventilation cap two doors one solid one glass high temperature grade foamed silicone rubber door gaskets over temperature and over current protection and sensor error detection Units are constructed with a durable powder coated steel exterior stainless steel interior two stainless steel shelves and glass wool insulation Units are also equipped with an internal 110V outlet for auxillary equipment Page 8 Incubator Shaker Floor LabCompanion BRDG Model 15 971 Incubated Shaker Cat AAH23336U
34. ndicator lights Press the right arrow until REPL HEPA XX is displayed Press the up down arrow to choose the number of months desired Press ENTE to save the number Press the MODE key to return to the RUN mode or right left to go to the next parameter To check the setting go to the CONFIG mode then right arrow until the NEW HEPA XX displays This is the number of days remaining until the replacement interval runs out A suggested interval of 6 months is the default setting however the interval may be adjusted due to environmental or other considerations LabSource Catalog 15497026 Manufactured By Thermo Forma Replacement in line filters Manufacturer s Part No 760210 Description NAPCO 8000 GAS FILTER PK Incubator accessories Gas Line Filters Incubator Accessory For Isotemp Large Capacity and 8000 Series CO2 Incubators Item No 15497026 130 40 PK SILCC CPLS Morrison 2 19 2013 HEPA Filter Information ThermoForma Part number 760175 SN TF0529411316 H Air Flow 4 CFM Resistance 44 inches w g Efficiency 99 97 0 3 microns 1 18 12 Info Main HEPA Filter For 3110 Series 310 Series w HEPA option Steri Cycle Series incubators Fisher Sci Cat 15 497 022 Thermo Scientific No 760175 Each for 66 03 Page 41 Incubators CO2 Thermo Warning Messages m 22 reminder set time expired REPLACE HEPA CU2 IS HIGH 15 min 15 RH is lower than RH Low L
35. of each operating mode Energy Mode When selecting the Energy mode the beeper will sound and the yellow indicator next to the digital display will illuminate The numbers on the display represent microjoules cm 100 Enter the specific microjoule level desired begin irradiation Stratagene recommends 120 000 microjoules for most membranes if selecting this amount the LED display will read 1200 If an error 15 made while entering the energy level press Reset to clear the display and then reenter the desired value Time Mode When selecting the Time mode the beeper will sound and the green indicator next to the digital display will illuminate The numbers on the display represent minutes Enter the specific length of exposure desired in minutes from 0 1 999 9 Autocrosslink Mode When selecting the Autocrosslink mode the beeper will sound and a preset exposure of 1200 microjoules 100 will be displayed No further entries are required The crosslinking will be complete in 25 50 seconds 5 50 5 11 008 41 Jic S Vereor 19 3 10 nate gadget Ext Notepad Bmorrison on Feclusterl AOL com Welcome to E http www artisan s 4 9 33 AM UV Crosslinking Standard Procedures Morrison p n e m gt gt Hate gadget Stratagene has found that a setting of 120 000 microjoules cm 1200 on the LED display is optimal fo
36. r attachment of RNA or DNA to nylon nitrocellulose or reinforced nitrocellulose membranes in any of the procedures listed above 1 Place one or two sheets of absorbent paper lightly dampened with transfer buffer such as 10x SSC on the floor of the Stratalinker UV crosslinker Place the membrane on top of the absorbent paper with the side with the attached nucleic acids facmg upwards enabling direct irradiation of the nucleic acids by the ultraviolet bulbs Note To obtain the best results place the membrane into the Stratalinker UV crosslinker while the membrane ts still damp but not dripping after the DNA or RNA transfer step 2 Close the door of the Stratalinker UV crosslinker 3 Press the button to initiate the desired function When the UV light bulbs turn on the display will immediately begin to count down from the entered value If the door is opened at any point during exposure the irradiation will stop and the remaining exposure value will be displayed Close the door to continue irradiation 4 When the irradiation is complete the beeper will sound for approximately 3 4 seconds The Autocrosslink setting will take approximately 25 50 seconds 5 Remove the membranes and close the door The irradiation is now complete Clean the floor of the Stratalinker UV crosslinker after each use to remove any residual salt Bmarrisan on Morrison Prot E AOL com w BIZ hkEn w
37. s transfer regulator Supply Tank Shut off valve Regulator Shut off valve Regulate pressure to instrument or device Regulator Coupling Nut E 2 ne we T amp 4 E ES 2 718 gt gt Turn Regulator shutoff valve on active tank Clockwise CW to full off position 2 Turn Tank shutoff valve on the current active tank Clockwise CW to full off position 3 Loosen the Regulator coupling nut with large pliers or wrench counter clockwise CCW to loosen 2 Ww 4i P Note on CO2 do not use washer Check for leaks using soap bubbles ON SPARE or NEW TANK 4 Add pipe thread teflon tape if available 5 Transfer the Regulator valve assembly to the spare or secondary full tank and tighten the coupling nut by turning it CW until resistance is felt Be careful not to strip the brass coupling nut or over tighten 6 Open the Regular shutoff valve by turning CCW to the full open position 7 Open the Tank shutoff valve by turning CCW slowly until a pressure of lt 20psi is achieved 8 Adjust the Regulator main valve to a setting of about 12 psi maximum on the meter 9 Verify and or reset CO2 pressure settings on the incubator and wait until desired set level is achieved STLCC CPLS Morrison 2 19 2013 Page 47 Crosslinker Stratalinker 1800 UV UV Stratalinker
38. sion indefinitely long which lays the ground for polymerase chain reaction Most commonly the pairs of nucleic bases A T and G C are formed of which the latter is more stable STLCC CPLS Morrison 2 19 2013 Page 51
39. units C and F Selectable Auto run or Manual run Audible visual and audio alarm OT and Door Open Temperature range Ambient 5 to 70 0 C Very good uniformity and stability Incolony sheath heater Magnetic type door sealing Easy and convenient door open and close Inner door glass No interference during outer door open Tempered safety glass and Sealing chamber perfectly Dial type handle for inner door Solid and Easy open and close Shelf bracket can be removed for easy cleaning with disinfectant Upgraded 3 set point memories in advance Save time without AUTO TUNING Internal tempered safety glass 5 mm Optional Perforated shelf Master On Off 25 Toggle Switch Present set Temperature Temperature STLCC CPLS Morrison 2 19 2013 Page 28 Incubator Bench LabCompanion FV Control Panel RUN lit when unit is of chamber chamber on and monitoring preset values for temp HEAT blinking or lit when heater is on A T flickers in auto tune mode EA 379 g W ON Lit in wait 2 mode for timer EE gaeooo 1 waiting off time DOOR Lit if door is open alarm if gt 1 min Heat will turn off AUTOTUNE Special test tune operation mode Lock hold for 3 sec to lock protect other keys hold again to release O T Lit if alarm and overheated START STOP Start or resume Note if inner
40. wer supply against unt serial plate They must match Flug service cord into the grounded electrical outlet Push the power switch to the position and turn the Overtemperature Thermostat to ts maximum position clockwise using a coin or flat edged tool Set Main Temperature Controller Enter desired set point temperature To enter set point mode on the controller press ether the Up or Down arrow pad one time The digital display will start to blink going fram bright to dim While blinking the digital display is showing the set point To change the set point use the Up and Down arrow pads If the arrow pads are not pressed for five 5 seconds the display will stop blinking and will read the temperature of the unit Mate that the Overtemperature Thermostat should be turned ta its maximum postion clockwise until the unit has stabilized at desired set point temperature Allow the incubator at least Z4 hours to stabilize Calibration t is recommended that calibration is once the unit installed In its working environment and has been stabile at set paint for several hours Place certified reference thermometer in the chamber certain the thermometer is nat touching any shelving Allow the temperature to stabilize aqain until the thermometer reads a constant value for one hour Compare the digital display with the reference thermometer If there 15 an unacceptable difference put the display into calibration mode by press
41. ww s Microsoft Pow 5 9 41 AM Incubator Hybridization Oven Rotation Fisher BRDG Fischer Biotech Model FBH110 110V Approx Dim 14 x 13 x 16 inches Hybridization Notes from Wikipedia is the process of establishing a non covalent sequence specific interaction between two or more complementary strands of nucleic acids into a single hybrid which in the case of two strands is referred to as a duplex Oligonucleotides DNA or RNA will bind to their complement under normal conditions so two perfectly complementary strands will bind to each other readily In order to reduce the diversity and obtain the most energetically preferred hybrids a technique called annealing is used in the laboratory practice However due to the different molecular geometries of the nucleotides a single inconsistency between the two strands will make binding between them less energetically favorable Measuring the effects of base incompatibility by quantifying the rate at which two strands anneal can provide information as to the similarity in base sequence between the two strands being annealed The hybrids may be dissociated by thermal denaturation also referred to as melting Here the solution of hybrids is heated to break the hydrogen bonds between nucleic bases after which the two strands separate In the absence of external negative factors the processes of hybridization and melting may be repeated in succes
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