HLA Antibody Luminex SOP OneLambda
Contents
1. BGNC bead background negative control serum fluorescent value for CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 12 of 15 Negative Control bead NC serum Negative Control Serum Cat LS NC validated for a given lot 10 2 10 3 of LABScreen beads For LABScreen PRA class and class II set positive NBG ratio cut off at gt 1 5 correlate well with positive reactions in the FlowPRA test For LABScreen Mixed set positive NBG ratio cut off at gt 2 2 correlate well with positive reactions in LAT Mixed For LABScreen Single Antigen calculate the following e The Working range NBG ratio maximum NBG ratio minimum e Relative NBG ratio cut off x working range NBG ratio minimum e Where x user defined percent cut off point within the working range for negative 1 gray area 2 weak positive 4 and strong positive 8 Positive negative cut off set at NBG ratio gt 15 of the working range NBG ratio calculated for each test serum gives results comparable to the LABScreen PRA assay 10 4 10 5 10 6 10 7 It is expected that the negative bead control NC value must be less than or equal to half of the positive bead control PC value Routinely expect a value less than 500 The positive bead control PC value should be greater than 500 and at least twice the NC bead value The PC NC ratio should be greater than 2 A lower ratio may be due to high NC backgro2. Place the probe in water in a sonicator bath for 2 5 minutes Replace sample probe and adjust the vertical height if necessary 9 PROCEDURE 9 1 Plate Preparation This procedure is the same for the LABScreen Single Antigen HLA Class or Class Il PRA Class or Class II and Mixed assay Only the bead set changes Note Sealing of the 96 well tray should be done carefully and completely to prevent well to well sample contamination especially when there is vortex step followed Seal the tray by pressing the seal against each rim of the 96 wells Do not re use tray seals Use a fresh seal for each step that requires application of a tray seal Step 1 Action Remove 1X wash buffer from storage Warm to room temperature 20 24 C for at least 1 hour prior to use Thaw serum samples and Negative Control serum LS NC at room temperature Assign a position on the 96 well plate for each of the sera and controls to be analyzed Document the positions see also Appendix Il Cover the unassigned wells of the plate with adhesive plastic plate cover Thoroughly vortex the bead vial to evenly resuspend the beads 0O AIO N Add 5 ul LABScreen beads to each test well Re vortex the beads every 2 minutes to keep the beads in suspension while distributing the beads CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 6 of 15 Add 20 ul of test serum to wells acc
3. e Remove seal carefully Remove wash buffer by flicking and blotting dry upside down 15 Repeat wash step 14 another time 16 Prepare fresh 1X dilution of PE goat anti human IgG conjugate 1 ul conjugate 99 ul 1X wash buffer per sample Store at room temperature in the dark Note To accommodate for pipetting losses prepare one additional volume of diluted conjugate CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 7 of 15 Vortexing the plate 10 seconds to disperse the beads Add 100 ul of 1X 17 conjugate anti human IgG to each well Carefully cover with a new tray seal and vortex 5 8 seconds Incubate for 30 minutes at room temperature in the dark on a rotating 18 platform 200 rotations per minute 19 Centrifuge at 1300 g for 5 minutes with no or low break 20 Remove seal carefully Remove conjugate by flicking and blotting dry 21 Repeat wash as in step 14 for 2 times 29 Vortexing the plate 10 seconds to disperse the beads Add 80 ul of 1X PBS to each well Carefully seal the plate and vortex 5 8 seconds Read plate on Luminex instrument 23 Note Tray may be stored up to 24 hours at 4 C in the dark if not acquired immediately 9 2 Instrument preparation Warm Up Prime Backflush Alcohol Flush and Wash Step Action 1 Turn on computer fluoroanalyzer and X Y platform 2 Double cli
4. Procedure 0001 Effective Date 02 15 11 Page 14 of 15 Appendix Il When assigning positions on the filter plate the following samples and sample order should be used to facilitating data analysis e ForLABScreen Mixed kit test all 20 samples in the following order negative EMWK1 EMWk2 EMWK3 EMWK4 EMWK5 EMWK6 EMWK7 EMWK8 EMWkg UCLA1 UCLA2 UCLA3 UCLA4 UCLA5 UCLA6 UCLA7 UCLA8 UCLA9 UCLA10 UCLA11 e ForLABScreen Single Antigen HLA Class and LABScreen PRA Class kits test 15 samples in the following order negative EMWK1 EMWkK2 EMWK3 EMWkK4 EMWK5 EMWK6 EMWK7 EMWkK8 EMWk9 UCLA1 UCLA2 UCLA3 UCLA4 UCLA11 e ForLABScreen Single Antigen HLA Class II and LABScreen PRA Class II kits test 17 samples in the following order negative EMWK1 EMWk2 EMWK3 EMWkK4 EMWK5 EMWK6 EMWkK7 EMWK8 EMWK9 UCLAS UCLA6 UCLA7 UCLA8 UCLAY UCLA10 UCLA11 CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 15 of 15 Appendix Ill The following nomenclature should be used for labeling sample runs and data batch files Patient list file name kit name TXT Batch folder name method used _ kit name _ center name Use the following abbreviation for method used o MAN for manual test of a current lot o AUTO for automation test of a current lot o Lot2 for manual test of a future lot Use the following abbreviation for kit names o LSM for LABScreen Mixed o PRA1
5. Reagent Formula Vendor amp Catalog Number Stock 100 Reagent alcohol Fisher Cat A962 4 70 Alcohol or comparable 70 Add 350 ml Alcohol to 150 ml Millipore water Note Ethyl or isopropyl alcohol may be used Luminex Store at 2 8 C up to manufacturer s expiration date e xMAP Classification Calibration Microspheres CAL1 Cat L100 CAL1 e xMAP Reporter Calibration Microspheres CAL2 Cat L100 CAL2 e xMAP Classification Control Microspheres CON1 Cat L100 CON1 e xMAP Reporter Control Microspheres CON2 Cat L100 CON2 Hazard Warning Contains Sodium Azide Use appropriate PPE and disposal precautions Consult MSDS PE Conjugated Goat Anti Human IgG 1000 tests One Lambda Cat LS AB2 Store in dark at 2 5 C up to manufacturer s expiration date Stock 100X Reconstitute with Millipore water Check the bottle label for the recommended volume to prepare stock This volume may vary lot to lot depending on the titer of antibody conjugate Centrifuge solution if not completely clear after standing at room temperature for Goat Anti Human IgG 4 2 hours Store at 2 5 C up to 6 months from reconstitution or manufacturer s expiration date if earlier Working 1X Add 1 ul 100X conjugate to 99 ul wash buffer Prepare fresh Store at room temperature in the dark up to 24 hours Return unused portion of 10X conjugate to storage for future use Calibrator and Control Microspheres Ha
6. for LABScreen PRA Class o PRA2 for LABScreen PRA Class II o SA1 for LABScreen Single Antigen HLA Class o SA2 for LABScreen Single Antigen HLA Class II Use the following abbreviation for institutes or centers participating in the workgroup o BWH for Brigham and Women s Hospital o EU for Emory University o UM for University of Manitoba o NWU for Northwestern University o UCLA for University of California Los Angeles o UP for University of Pittsburgh o WUSL for Washington University St Louis For example AUTO_SA1_NWU stores output and csv files from automation test using LABScreen Single Antigen class kits generated by Northwestern University The patient list used by this batch test is SA1 TXT CTOT sop0001 doc
7. kit products at or below 65 C up to manufacturer s expiration date Once thawed do not refreeze Store beads and wash buffer at 2 5 C up to three months or manufacturer s expiration date if earlier Hazard Warning Contains Sodium Azide LABScreen Mixed One Lambda Cat LSM12 Kit includes Class Class Il and MICA Antigen Beads and 10X Wash Buffer Store unopened kit products at or below 65 C up to manufacturer s expiration date Once thawed do not refreeze Store beads and wash buffer at 2 5 C up to three months or manufacturer s expiration date if earlier Hazard Warning Contains Sodium Azide LABScreen Negative Control Serum One Lambda Cat LS NC 20 tests Store frozen 20 C or below until first usage After thawing keep at 2 5 C up to 5 days Freeze aliquots or re freeze the original reagent vial 1time only if reagent is not used within 5 days Use before manufacturer s expiration date Wash Buffer LABScreen 10X Wash Buffer 13 ml bottle One Lambda Cat LSPWABUF Already included in the kits above Stored at or below 65 C together with the beads up to manufacturer s expiration date Once thawed do not refreeze Dilute 13 ml 10X buffer in 117 ml Millipore water to make 1X work solution Store at 2 5 C up to three months or until expiration date CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 4 of 15 10X D
8. CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 1 of 15 HLA ANTIBODY DETECTION LUMINEX One Lambda 1 PURPOSE This procedure describes the steps required to perform solid phase immunoassay to qualitatively detect IgG HLA Class and Class II antibodies and their specificities 2 SCOPE This procedure applies to both the screening and specific antibody identification assays utilizing One Lambda LABScreen Mixed LABScreen PRA Class LABScreen PRA Class II LABScreen Single Antigen HLA Class and LABScreen Single Antigen HLA Class Il combination for the Luminex 100 flow cytometer 3 DEFINITIONS AND ACRONYMS N A 4 RELATED PROCEDURES N A 5 SPECIMEN 5 1 Acceptable e Serum separated from fresh whole blood collected without anticoagulant using aseptic technique Store serum aliquot at 2 to 8 C until tested or freeze at 20 to 80 C for longer storage Separate serum from blood cells when stored or shipped to avoid hemolysis see also Appendix I 5 2 Unacceptable e Serum contaminated with bacteria immune complexes or other immunoglobulin aggregates If present these can cause increased non specific binding and erroneous results Note Aggregates may be removed by centrifugation or filtration prior to testing e Serum repeatedly frozen and thawed This can weaken antibody reactivity CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 2 of 15 6 REAGENTS
9. ck Luminex IS icon Warm Up Click Warm Up button located under the Home tab Click OK to continue The warm up procedure takes approximately 30 minutes Upon completion the Laser Status area in the status bar becomes green and the display reads Warmed Up Note Once instrument is warmed up the laser automatically shuts down if not used for 4 hours Check sheath fluid and waste reservoirs Fill sheath fluid to the blue line near the top The level must not fall below the bottom tubing Empty the waste reservoir to avoid back up into the system Prime Click Prime under the Maintenance tab to prime the instrument and remove air from the system s fluidic pathways Click OK to continue Upon completion the command list indicates whether the command succeeded with green text or the command failed with red text Note Prime 2 times before next step Backflush To remove obstructions from the cuvette Perform this step once a month to prevent system clogging Click Backflush Click OK to continue Upon completion the command list indicates whether the command succeeded with green text or the command failed with red text Alcohol Flush Click Eject Retract to eject the plate holder Add 70 alcohol into the black reservoir Click the Alcohol Flush OH Flush button under the Maintenance tab CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 8 of 15 Clic
10. da software to be able to analyze Luminex output Test Sort order could be either Alphabetical by Test name or Template Setup Order 14 Click OK to confirm change of options 9 5 Read Plate Step Action 1 Click Eject Retract and load plate into XY platform 2 Press Start Program begins 3 Luminex software saves the data as csv output files on the computer at completion of each run 9 6 Instrument shutdown Sanitize Wash and Soak Functions Wash Click Wash button under the Maintenance tab A confirmation 1 dialog box appears Click Eject Retreat Place sheath fluid into the reservoir or well indicated 2 Click the drop down arrow to the right of the Wash button An image of the CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 11 of 15 microtiter plate and reservoir in the plate holder appears 3 Select the location for the wash solution and click Eject Retract Click OK When the wash is complete the command list indicates whether the 4 command succeeded with green text or the command failed with red text Note Wash 2 times with sheath fluid 5 Sanitize Click Sanitize 6 Click Eject Retract to eject the plate holder Add 20 bleach to the reservoir and click Eject Retract to retract the plate 7 holder Click OK to continue Upon completion the command list indicates
11. g box appears Click OK and wait until CON2 completes The device status section in the status bar changes from Running to Standby 16 Note The message log on the Diagnostics tab shows the status of the CON1 and CON2 commands If an error occurs during system calibration the Diagnostics tab turns red 17 Click Wash to wash the system with sheath fluid after running the system calibrators A Confirmation Screen dialog box appears Click OK and wait until the wash completes The device status section in the status bar changes from Running to Standby 18 Note If an error occurs during system calibration an X appears in front of the Diagnostics tab title and the text turns red The command list on the Run Batch tab also reports that the command failed 19 If calibration is unsuccessful contact the supervisor If required contact vendor technical support 20 Print and file System Control Trend calibration report 9 4 Batch Set Up Step Action Download the acquisition template file for the kit and lot from OneLambda 1 website In Luminex100 IS software click Import Template under File menu and choose the template file 2 Single Locus Click New Batch under the Home tab to display template options 3 Double click to select the appropriate template 4 Type the batch name in the upper left corner 5 Click Load Patient List on the right Import the appropriate patient list from the desired folder The sample z numbers load a
12. k Eject Retract to retract the plate holder Click OK to continue Upon 8 completion the command list indicates whether the command succeeded with green text or the command failed with red text 9 Click Eject Retract to eject the plate holder Remove alcohol from the reservoir Fill with sheath fluid 10 Wash Click Wash button under the Maintenance tab A confirmation dialog box appears 11 Click the drop down arrow to the right of the Wash button An image of the microtiter plate and reservoir in the plate holder appears 12 Select the location for the wash solution and click Eject Retract Click OK When the wash is complete the command list indicates whether the 13 command succeeded with green text or the command failed with red text Note Wash 2 times with sheath fluid to following alcohol flush 14 The machine is ready for calibration 9 3 or batch set up 9 4 9 3 Calibration Calibration should be performed weekly or in circumstances mentioned in 8 5 Step Action 1 Vortex the calibration beads before use 2 Aliquot 2 3 drops of the calibration beads into designated wells 3 Click Eject Retract from the toolbar to eject the plate holder 4 Place the plate on the plate holder Click Eject Retract to retract plate Run System Calibrators Click New CAL Targ from the Maintenance tab to enter or confirm the calibration lot numbers Enter the values from the Certificates of Analysis inc
13. luded with the calibrators into the CAL1 and CAL2 entry fields in the Update CAL targets dialog box 6 Click OK to close dialog box Click CAL1 from the Maintenance tab A Confirmation Screen dialog box i appears g Click OK and wait until CAL1 completes The device status section in the status bar changes from Running to Standby 9 Click CAL2 from the Maintenance tab A Confirmation Screen dialog box appears Click OK and wait until CAL2 completes The device status section in the status bar changes from Running to Standby 10 Note The message log on the Diagnostics tab shows the status of the CAL1 and CAL2 commands If an error occurs during system calibration the Diagnostics tab turns red CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 9 of 15 Run System Controls Click New CON Targ from the Maintenance tab to 11 enter or confirm the system control lot numbers Enter the values from the Certificates of Analysis included with the system controls into the CON1 and CON2 entry fields 12 Click OK to close dialog box 13 Click CON1 from the Maintenance tab A Confirmation Screen dialog box appears 14 Click OK and wait until CON1 completes The device status section in the status bar changes from Running to Standby 15 Click CON2 from the Maintenance tab A Confirmation Screen dialo
14. ording to plate format Pipette up and down 3 times to mix Avoid generating bubbles Note Centrifuge serum for 5 minutes at 10 000 x g before adding to test well Add 20 ul Negative Control serum LS NC to one well per batch run Pipette up and down 3 times to mix Avoid generating bubbles Note Centrifuge serum for 5 minutes at 10 000 x g before adding to test well Cover the plate with adhesive tray seal by pressing the seal against each rim of the 96 wells Wrap the plate in foil or cover with a box to protect from light Incubate for 30 minutes at room temperature in the dark on a rotating platform 200 rotations per minute Return unused portions of control sera and beads to 4 C for future use Note For LS NC thawed for the first time from One Lambda shipment make 3 aliquots and freeze back to 20 C 10 Remove seal Add 150 ul of 1X wash buffer to each test well Cover with a new tray seal 11 Centrifuge at 1300 g for 5 minutes with no or low break 13 Remove seal carefully Remove wash buffer by flicking once Press plate upside down against a stack of paper tower to blot dry 14 Wash e Vortexing the plate 10 seconds to disperse the beads e Add 200ul of 1X wash buffer to each well Carefully Seal the plate with adhesive tray seal by pressing the seal against each rim of the 96 wells e Vortexing the plate 5 8 seconds Centrifuge at 1300 g for 5 minutes with no or low break
15. rrors or reagent failures have occurred 8 3 Daily instrument maintenance when in use e Alcohol flush to remove air bubbles from the cuvette e Check that vertical alignment of sample probe is appropriate for the plate used with the kit in use e After running samples sanitize with 20 bleach CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 8 4 8 5 Effective Date 02 15 11 Page 5 of 15 Perform Soak function at the end of each day to prevent salt crystals from forming in the probe Click Eject Retract to eject the plate holder Add at least 250 ul distilled water in well D1 Click again to close Click OK to continue Weekly instrument check Open instrument doors and visually inspect the analyzer for leaks corrosion and other signs of improper instrument function Check all visible tubing connections Check the XYP air intake filter for buildup of dust Run calibration beads and system controls weekly Additionally repeat calibration following Instrument relocation If system power turned off If a part is replaced If the delta calibration temperature shown on the system monitor is more than 3 degrees NOTE Run controls after each calibration 8 6 Monthly instrument maintenance Clean sample probe Remove probe Unsnap the light housing above the probe Unscrew the Cheminert fitting on top of the probe Gently grasp the probe and push up Remove the probe out of the top of the sample arm
16. ulbecco s Phosphate Buffered Saline PBS without Ca Mg Irvine Scientific Cat 9242 or equivalent Store at room temperature up to Water PBS manufacturer s expiration date 1X PBS Dilute 10X stock 1 10 with Millipore water Adjust pH to 7 4 Store up to one year at 4 C LAB MAP Sheath Fluid Luminex Corporation Cat 40 Sheath Fluid 50000 Store at room temperature up to manufacturer s expiration date Millipore Clinical Lab Reagent Water CLRW Deionized purified water 7 EQUIPMENT AND SUPPLIES 7 1 Equipment Item Vendor amp Catalog Number Analysis Software HLA Fusion Software One Lambda Inc Luminex 100 Luminex 100 IS Flow Analyzer Luminex Corporation Rotator Rotating platform 200 rotations per minute Various vendors Centrifuge Beckman TJ6 or comparable Vortex Fisher Vortex Genie 2 or comparable 7 2 Supplies Item Vendor amp Catalog Number 96 well plate U Corning 96 wells 330ul microplate Cat 3799 or bottom equivalent Tray Seal Tray Seal One Lambda Cat SSPSEA300 Microcentrifuge tubes ISC BioExpress Colorless tubes Replace last digit in order number for colors G green Y yellow O orange 1 6 ml Cat C3300 1 or equivalent 8 QUALITY CONTROL 8 1 Specimen preparation Centrifuge for 5 minutes at 10 000 x g prior to testing 8 2 Include the negative control sera with each test to determine if technical e
17. und for the serum a high HLA bead signal for the NC control or a low signal from the secondary antibody or from the LABScan 100 system directly The following results require evaluation by the Supervisor or Director prior to reporting Samples with a bead count less than 50 NC bead value greater than or equal to 1500 PC bead value less than or equal to 500 PC NC ratio less than or equal to 2 11 REFERENCES Luminex 100 IS User s Manual Version 2 0 Luminex Corporation 2001 LABScreen Product Insert One Lambda Inc Rev 17 12 SUPPLEMENTAL MATERIALS N A CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 13 of 15 Appendix 1 2 3 Serum samples were distributed in 1ml or 2ml aliquot on dry ice Sera should be stored at 80 C upon receiving To avoid repeated freeze thaw cycles during the period of study sera should be thawed once on ice thoroughly mixed by pipetting for 5 8 times and aliquoted 100 ul vial into 0 5 ml eppendorf Then sera should be frozen and stored at 80 C No more freeze thaw cycle will be accepted during the period of study When measuring HLA antibodies sera should be thawed at room temperature mixed by pipetting for 5 8 times and centrifuged 10 000 x g for 5 minutes Sera are kept at room temperature until use Unused sera should be stored at 2 8 C for no longer than 48 hours or discarded CTOT sop0001 doc CTOT HLA Antibody Testing
18. utomatically into the well template see also appendix III Note To move the sample list click on the first sample and drag to the proper well The other samples will align behind the first CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 10 of 15 7 Click Finish and go to step 13 8 Multiple Loci Click New Multi Batch under the Home tab 9 Click New Batch to display template options 10 Select appropriate template and follow steps 3 6 above Click Finish 11 Repeat steps 9 10 for each batch 12 Name the overall multi batch then click Finish To make sure the output is capable of being loaded into HLA Fusion for analysis purpose set the Luminex machine options under tools as the following Under General tab Default Batch Directory specify directory for CSV file storage make Analysis Display Digits 2 Check Display Confirmation Screens Check Enable Raw Data Storage Check Report Raw Fluorescence Under Data Export tab check the following Auto Export Batches Copy Output CSV to Common Ourput Dir All options under Additional Export Stats Alphabetical by test name under Test Sort order All options under Additional Batch Information Both 1 A1 2 B1 3 C1 under Export Location Label Style 13 Note Report Raw Fluorescence Trimmed Count Trimmed Mean and the first four options under Additional Batch Information are required for OneLamb
19. whether the command succeeded with green text or the command failed with red text 8 Wash Perform wash step 1 4 for a total of 3 washes with distilled water 9 soak solution distilled water Soak Click Soak A dialog box appears indicating the location to place Select a location from which to draw fluid using the microtiter plate and 19 reservoir image displayed Click Eject Retract 11 Verify the selected location is empty fill with distilled water and click Eject Retract Click OK to continue The command list on the Run Batch tab indicates that it is soaking Wait until the soak command completes before initiating 12 another command Upon completion the command list indicates whether the command succeeded with green text or the command failed with red text Note Soak 2 times with distilled water 13 Turn off instrument and computer 10 INTERPRETATION 10 1 To determine if a bead is positive Normalized Background Ratio NBG ratio is calculated as the following For LABScreen PRA or LABScreen Single Antigen NBG ratio S N SNC bead BG N BGNC bead For LABScreen Mixed NBG ratio S N SNC bead BG N BGNC bead Note If BG N BGNC bead lt 50 then use 50 as a default threshold value S N sample specific fluorescent value for bead N SNC bead sample specific fluorescent value for Negative Control bead BG N background negative control serum fluorescent value for bead N
20. zard Warning Contains Sodium Azide One Lambda Cat LS1A04 Kit includes Class Single Antigen Beads and 10X Wash Buffer Store unopened LABScreen Single kit products at or below 65 C up to manufacturer s Antigen HLA expiration date Once thawed do not refreeze Store Class Combi beads and wash buffer at 2 5 C up to three months or manufacturer s expiration date if earlier Hazard Warning Contains Sodium Azide CTOT sop0001 doc CTOT HLA Antibody Testing Procedure 0001 Effective Date 02 15 11 Page 3 of 15 LABScreen Single Antigen HLA Class Il Group 1 One Lambda Cat LS2A01 Kit includes Class II Single Antigen Beads and 10X Wash Buffer Store unopened kit products at or below 65 C up to manufacturer s expiration date Once thawed do not refreeze Store beads and wash buffer at 2 5 C up to three months or manufacturer s expiration date if earlier Hazard Warning Contains Sodium Azide LABScreen PRA Class One Lambda Cat LS1PRA Kit includes Class Antigen Beads and 10X Wash Buffer Store unopened kit products at or below 65 C up to manufacturer s expiration date Once thawed do not refreeze Store beads and wash buffer at 2 5 C up to three months or manufacturer s expiration date if earlier Hazard Warning Contains Sodium Azide LABScreen PRA Class Il One Lambda Cat LS2PRA Kit includes Class II Antigen Beads and 10X Wash Buffer Store unopened
Download Pdf Manuals
Related Search