User Manual - Sage Science
Contents
1. cassette product being used Master run Cassette protocols list Definitions 1 PX152 2 PROO272 f 4 Off Line 5 Off Line 7 Off Line Configure Pippin Link v 1 01 os fy 11111 a 7 lt _ 082412 new membrane control 2prcnt EtBr TS 6 75 Marker D Target tight cuts only 082412 new membrane lot080812 2prcnt range run4 TS 5 Marker A No Overflow Detection 8 22 12 test M epicentre 3 Marker C Band Capture only Test Folder Test Protocol 3 Marker C No Overfiow Detection 3 DF Marker H 3 Select the protocol to be shared by the group The protocol will be highlighted in blue The associated cassette definition will automatically highlight sage science 15 x 10 978 922 1832 www sagescience com support sagescience com 4 Press the Share Protocol button The will load the protocol on all instruments in the group The selected protocol will appear in the associated Protocol field in the group status area Share Protocol appears Share Protocol in status area butto Pippin Link v 1 01 ia 215 a Pxis2 2 PR00272 7 3 Ofline 5 Off Line l 6 OffLine 7 Ofline 7 8 Offline Configure ig 45 09 19 12 SH testing 091912 SH testing duster 1 duster 1 D Sees E nil ll o O rm i 082412 new membrane control 2prent EtBr TS 0 75 Marker D Target tight cuts only 082412 new membrane lot0808 12 2prent range run4 TS 1 5 Marker A2. instrument to hub e Calibration fixture e Rinse Cassette e International plug adapter sage science 978 922 1832 www sagescience com support sagescience com 2 Set up Set up of the Pippin Link control bundle requires connecting the instruments and laptop to the Ethernet hub Addresses are set in the software application and the connections to not need to be made in a sequential order However it is recommended though not essential that all devices are completely powered up the Pippin instruments should powered and the software launched as outlined in the instrument user manual Use the following guidelines 1 Connect the dummy VGA connectors to the video monitor ports found on the back panel of the Pippin instruments 2 Connect the Pippin power supplies to the back panel of the Pippin instruments 3 Note the serial number on the back panel of the instrument Place the Pippin Instruments on the lab bench in ascending order by serial number In the software the lowest serial number will map to Tab 1 etc 4 Plug the Pippin power supplies into electrical outlets 5 Power up each Pippin instrument by pressing the power buttons in the rear of the instruments 6 Connect the power supply for the Ethernet Hub and plug in the Ethernet power supply into an electrical outlet 7 Connect the power supply for the laptop computer and power up the computer 8 With Ethernet cables connect the Pippin instruments from the Ether
3. Close the lids on each instrument in the group ccceeeccccssccceeesececeesececeeescceeeuseceseuaeceeseeeeeeees 11 7 Press the Calibrate DUTO gasicucccnceonvecasaensaers En EaR A EEEE 12 8 Open the instrument lids and remove the optical calibration 6 13 9 inspect and prepare TNE gel 0 aE E NEEE Ea 13 10 Place the gel cassettes into the instruments tO be 13 11 VSS E o e E E E E E E E A EE A EE E ET E 13 12 Pressthe Test Continuity DUTTON 8 13 13 Open the lids TO all 00 00 E 14 14 LOA samples and or Markers coudsvsainsaunctencadnintoseans daixisdaacuinnuedhantdutbiaseasiinveigadlinrsaneimennousatedausiiares 14 15 Close the lids to all the inNStrUMents neisisissserisiscsrsireesesiirereronissessiii niesi erer innesti ir ninen 14 16 Pros aaan GOUD a mE ne Une er tan a en RE Oe en E ere er cee een 14 sage science 2 978 922 1832 www sagescience com support sagescience com Introduction Thank you for purchasing the Pippin Link control bundle Pippin Link includes all items required for the operation of group of Pippin Prep or BluePippin instruments from a single laptop computer The Pippin Link will operate 1 to 7 units simultaneously Specially modified Link equipped Pippin instruments must be ordered Please note the following important considerations e The Pippin Link control bundle and Pippin instruments will arrive preconfigured and user installable e Pippin Prep and BluePippin instrumen
4. No Overflow Detection 091912 2 DF duster test 091912 SH testing duster 1 100312 duster test SH1 8 22 12 test M epicentre 3 Marker C Band Capture only Test Folder Test Protocol 3 Marker C No Overfiow Detection 3 DF Marker H 91 5 Place the calibration fixture on the optical nests of each instrument in the group The dark side of the fixture should face down and completely cover the optical detectors Proper placement of the calibration fixture 6 Close the lids on each instrument in the group sage science 11 978 922 1832 www sagescience com support sagescience com 7 Press the Calibrate button The group status window will show the message Calibrating during the process Group status field will display Calibrating Calibrate Group button Pippin Link v 1 01 4 Off Line 6 Off Line 8 Off Line B 082412 new membrane control 2prent EtBr TS ge 0 75 Marker D Target tight cuts only 082412 new membrane lot080812 2prent range run4 TS 1 5 Marker A No Overflow Detection 1 5 DF Marker K 2 Marker B No Overflow Detection 100312 duster test SH1 2 EF Marker E v2 8 22 12 test M epicentre 3 Marker C Band Capture only Test Folder Test Protocol 3 Marker C No Overflow Detection 3 DF Marker H the calibration has been successful the Group Status field will display the message idle cal If the calibration has failed the message will revert to idle Pippin Lin
5. User Manual LINK Control bundle for multiple Pippin Prep or BluePippin instruments Version 1 01 Part No LNK 0001 978 922 1832 www sagescience com support sagescience com Sage Science Inc Suite 3150 500 Cummings Center Beverly MA 01915 2012 Sage Science Inc All rights reserved Sage Science Pippin Prep BluePippin and Pippin Link are trademarks of Sage Science Inc All other brands and name mentioned herein are property of their owners sage science 978 922 1832 www sagescience com support sagescience com Table of Contents SNE 3 1 KINE E TE 4 5 6 2 6 0 THEM IN SCOE 0 6 AS LAAN E OS E E E A IE EEE E E E E EN 7 TheM trunen Inter ICE sea 8 4 Using the Pippin Link Control Interface cc cccccccssecccceseccecesececeeececceeecceeseseceseuneceeseneceesensecetsgneess 9 L Select the master instrument sssssssssreresssssrsrrrresssssereresssssssreresessssettereesssssetrreeessssereresesssseene 9 2 Select the group 6 9 3 Select the protocol to be shared by the group eessssssssssessesesrerssrreessrrrssrreessrrressreresreressreresreresene 10 4 Press the Share Protocol button sssessessssssereressssssrreressssssssrrreessssserrreessssserrreressseeeerreessseeere 11 5 Place the calibration fixture on the Optical nests cccceseccccsssccecesseceeeesececceececeeeeeeessuseeetseneees 11 6
6. e Name field The default name is the instrument serial number The name that appears in this field will appear in the Tab heading in the Pippin Link interface sage science 978 922 1832 www sagescience com support sagescience com 4 Using the Pippin Link Control Interface 1 Select the master instrument One Pippin in the group of instruments must be selected a master The protocols that are stored on the master instrument will be used to operate the group 2 Select the group instruments This should include all instruments to be run as a group including the master Select master and group instruments Pippin Link v 1 01 aj pxis2 2 PR00272 7 3 Offline Parar p 23 Oct 2012 09 21 45 DISPLAY PROGRAM INFO EXIT W O SHUTDOWN Pippin Link v 1 01 master ide WO O de 091912 SH testing 091912 SH testing duster 1 duster 1 Instruments 1 and 2 are the group sage science 978 922 1832 www sagescience com support sagescience com When the master instrument is selected the protocols stored on the master instrument will be displayed in the Protocols With new instruments this field will be empty Refer to the Pippin Prep or BluePippin Operations Manual for instructions on programming protocols The cassette definitions are shown in the Cassettes field These are default definitions and must match the gel
7. faces Pippin Link v 1 01 1 PX152 i ERTS 3 Off Line 4 Off Line 5 Off Line O OTe P L L L 000 Sa 23 Oct 2012 09 21 45 DISPLAY PROGRAM INFO EXIT W O SHUTDOWN Pippin Link v 1 01 TI PXIS Instrument Serial Number appears in Tab heading d Pippin Link v 1 01 Up MEI 2 PROO272 Group Status will display Idle sage science 7 978 922 1832 www sagescience com support sagescience com The Instrument Interface Instrument Tab will provide access to the local instrument interface Refer to the Pippin Prep or BluePippin operations manual for detailed instructions on monitoring programming and analysis of Pippin DNA size selection 2 Worst 6 Offline 00008 Oone Main Protocol Editor Log Review File Man ager System 0 ptions Sample ID Current Elution Timer Reference Idle Separate Elute RE ceo eel he hae 3 O A E o ee gp 1 1 1 1 I 1 1 U 1 00 00 01 00 02 00 03 00 04 00 05 00 06 00 07 00 08 00 Time hh mm Protocol Name 091912 SH testing cluster1 2 Progress hh mm ss Voltage Y Clock Remote Access 1 4 02 Nov 2012 13 55 20 TEST START 1 02 00 03 00 04 00 05 00 06 00 07 00 08 00 sage science Time hh mm CALIBRATE f SNAPSHOT INFO SHUTDOWN Note In the instrument name may be edited in the System Options tab in th
8. k v 1 01 Group Status will display Idle cal sage science 12 978 922 1832 www sagescience com support sagescience com 8 Open the instrument lids and remove the optical calibration fixtures 9 Inspect and prepare the gel cassettes to be run on each instrument as outlined in the Pippin Prep and BluePippin Operations manuals 10 Place the gel cassettes into the instruments to be run 11 Close all lids 12 Press the Test Continuity button The group status window will show the message continuity during the process Group status field will Test Continuity button Pippin Link v 1 01 a T x Group Contro U pxis2 2 PR00272 lG Offline a Offline l5 Offline l 6 Offline 7 Off Line 6 Offline Cofffigure ETT ee oe display continuity 082412 new membrane control 2prent EtBr TS 0 75 Marker D Target tight cuts only 1 5 Marker A No Overflow Detection 1 5 DF Marker K 2 Marker B No Overflow Detection 8 22 12 test M epicentre Test Folder Test Protocol the continuity test has been successful the Group Status field will display the message idle cal If the calibration has failed the message will revert to idle cal gt Pippin Link v 1 01 5 2 PROO272 Group Status will display ready sage science 13 978 922 1832 www sagescience com support sagescience com 13 Open the lids to all instr
9. net ports on the back panel of the instruments to the Ethernet ports on the Ethernet Hub The cables do not need to be connected in any order into the hub The instruments will be auto detected by the Pippin Link software according to IP addresses that have been preset prior to shipment 9 With an Ethernet cable connect the laptop to the Ethernet Hub 10 Launch the Pippin Link Application on the laptop PC sage science 978 922 1832 www sagescience com support sagescience com 3 Software Overview The Main Screen When the Pippin Link application has been started the following screen will launch The Main screen displays the status of every Pippin in the group The adjacent tabs if pressed will display the software interface in the individual Pippin instruments in the group Group Status Pippin Link v 1 01 eyo ety Wel 2 l Offline 4 Offline l 5 Offline l 6 Offline Sule Ane l 8 Offline B ll 9 23 Oct 2012 09 21 45 ETA 4 Group Commands sage science 6 978 922 1832 www sagescience com support sagescience com Instrument Tabs The Pippin instrument software interface is accessed by pressing The Tabs on the main screen Ifan instrument in connected to the group the instrument s serial number will appear in the Tab heading When an instrument is connected to the Pippin Link the Group Status window will display the message Idle Instrument inter
10. ts cannot be operated within the same group due to the incompatibility of cassette definitions e Installing additional instruments that are not link equipped to a Pippin Link group requires hardware and software modifications to the instrument e Removing instruments from a Pippin Link group for the purpose of stand alone use will required the installation of a monitor and keyboard and mouse These items are not included with each link equipped instrument purchases However a monitor keyboard and mouse are included with the Pippin Link control bundle sage science 3 978 922 1832 www sagescience com support sagescience com 1 Unpacking The Pippin Control Bundle LNK 0001 will arrive with the following components e Windows based laptop computer with Pippin Link software installed The application icon will appear on the desktop e 8 Port Ethernet Hub Switch Netgear FS108 or similar e ethernet cable to connect the laptop to the hub e Pippin Link user manual this document e For stand alone Pippin operation not needed for Pippin Link operation o Computer monitor 0 Keyboard mouse and mouse pad ts Fippin Link Controller Pippin Link Desktop Icon Pippin link equipped instruments will arrive PIP O002 or BLU0002 with the following components e Pippin Prep L or BluePippin L instruments e Pippin Prep or BluePippin user manual e Dummy VGA monitor connector e Instrument Power Supply e Ethernet cable connects
11. uments 14 Load samples and or markers at outlined in the Pippin Prep or BluePippin operations manuals 15 Close the lids to all the instruments 16 Press Start Group Start Group button Pippin Link v 1 01 ici 1 PX152 2 PROO272 6 Off Line 8 Off Line Configure otoco 091912 SH testing 091912 SH testing duster 1 duster 1 082412 new membrane control 2prent EtBr TS 5 0 75 Marker D Target tight cuts only 082412 new membrane lot080812 2prent range run4 TS 1 5 Marker A No Overflow Detection 100312 duster test SH1 8 22 12 test M epicentre Test Folder Test Protocol 17 The group may be paused restarted or aborted sage science 14
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