BioPette Plus Single Channel User Manual 2010
Contents
1. PLUS BioPette Labnet International Inc PO Box 841 Woodbridge NJ 07095 USA Phone 1 732 417 0700 Fax 1 732 417 1750 email labnet labnetlink com www labnetlink com BioPette lt BioPette 6B 6C 5000 5000 ul 10000 10000 ul 1 D D 5mm 1 5000 D 6mm 10000 D 10mm gt BioPette CONTENTS 1 INTRODUCTION 2 SETTING THE VOLUME 3 ASPIRATING AND DISPENSING LIQUIDS 4 PRE RINSING 5 DENSE AND VISCOUS LIQUIDS 6 LABNET PIPETTE TIPS 7 RECOMMENDATIONS 8 RECALIBRATION 9 TROUBLESHOOTING 10 CLEANING AND STERILIZATION 11 PIPETTE KIT AND ACCESSORIES 12 SPARE PARTS 1 INTRODUCTION The BIOPETTE PLUS is a volumetric instrument de signed to measure and transfer liquids precisely and safely2. To remove the tip ejector in models BPP5000 and BPP10000 remove the ejector pushbutton Fig 3N and using a screwdriver unscrew the tip ejector by turning the screwdriver counter clockwise Damage to the piston or seal due to prolonged use with the aggressive liquids Disassemble the pipette as described above Replace the piston seal and O ring see Section 12 Rinse the inside of the shaft in distilled water and dry Lubricate the seal and O ring with the lubricant that has been included with each pipette The replacement of the piston requires conducting of calibration procedure Note The parts of BPP2 and BPP10 pipette should be lubricated evenly with a minimum amount of lubricant The pipette is reassemled improperly Disassemble the pipette and reassemble it observ ing the proper sequence of steps Fig 3 No lubricant on the sealing elements Remove the tip ejector Unscrew the shaft nut remove the shaft piston assembly seal and O ring Rinse the removed parts in distilled water and dry thoroughly Lightly lubricate the inside surfaces of the seal and the O ring with the included lubricant Reassemble the pipette in the reverse order Contamination of the inside of the pipette caused by extensive aspiration of chemically aggressive liquids or because liquid got inside the pipette Remove the tip ejector Unscrew the nut remove the shaft piston assembly seal and O ring Rinse the removed parts with distilled w
3. adjustment knob Fig 1B 4 BioPette to the direction diminishing counter readings to the required volume Before achieving the required volume slowly rotate the knob and observe carefully diminishing reading to avoid accidentally passing the setting value f the desired volume is higher than set volume shown by the counter the operator should turn the pipetting push button Fig 1A2 or the adjustment knob Fig 1B incre asing the value until the lower figure wheel comes 1 3 of a turn beyond the required setting and then slowly back ward until the setting reaches the desired volume Make sure not to pass the setting value If the knob is accidentally turned too far the process must be repeated The desired volume must always be set from the higher value in the order of decreasing value Following volume adjustment set the locking ring into the upper position thus locking the knob and preventing accidental volume change 3 ASPIRATING AND DISPENSING LIQUIDS Place a tip on the shaft of the pipette See Section 6 for the appropriate tip Press the tip on firmly using a slight twisting motion This will ensure an airtight seal Important Never aspirate liquids into the BIOPETTE PLUS without a tip attached Aspiration Press the pushbutton to the first positive stop Fig 2A Holding the pipette vertically immerse the tip into the sample liquid The depth to which the tip is immersed in the sample liquid depend
4. with the blue label on the pushbutton Tips 5000 These tips are used for volumes between 1000 ul and 5000 pl They are used with the BPP5000 models Tips 10000 These tips are used for volumes between 1000 and 10000 ul They are used with the BPP10000 models 7 RECOMMENDATIONS Observing the following recommendations will ensure maximum possible accuracy and precision of liquid sam pling Make sure to operate the BIOPETTE PLUS slowly and smoothly The depth of immersion in the sample liquid should be the minimum necessary and should remain constant during aspiration The BIOPETTE PLUS should be held in a vertical posi tion Change the tip when volume setting is changed or when a different liquid is to be aspirated Change the tip if a droplet remains on the end of the tip from the previous pipetting operation Each new tip should be pre rinsed with the liquid to be pipetted Liquid should never enter the BIOPETTE PLUS shaft To prevent this Press and release the pushbutton slowly and smoothly Never turn the pipette upside down Never lay the pipette on its side when there is liquid in the tip Never force the volume setting beyond its recommend ed limits When pipetting liquids with temperatures different from the ambient temperature it is recommended to pre rinse the tip several times before use Do not pipette liquids with temperatures above 70 BioPette When pi
5. 0032 1 0033 1 0033 23 1 0034 1 0035 1 0036 24 1 0037 1 0038 1 0038 25 1 0039 1 0040 1 0040 If the average aspirated volume exceeds the permissible value the following should be done Remove the pipetting pushbutton Fig 4A Warning The pipetting pushbutton consists of 2 parts the knob Fig 1A2 and the pushbutton Fig 1A1 After removal of the pushbutton both parts are separated Holding the volume adjustment knob to protect it against rotation insert the calibration key into the cuts of the calibration screw Fig 4B BioPette Turn the key clockwise to reduce the aspirated volume or counter clockwise to increase the volume One full turn of the calibration key changes the pipette aspira tion volume by the amount given in the table Fig 4C Take out the key and fix the pipetting pushbutton Fig 4D The pipetting pushbutton should be fixed by placing the knob on the arbor first Fig 142 and then the pushbutton Fig 1A1 Determine the average aspirated volume The average volume should be within the permissible range given in the table If the volume exceeds the values stated the recalibration procedure should be repeated When pipetting liquids with physical properties conside rably different from those of water follow the rules given in section 5 9 TROUBLESHOOTING If you notice an improper pipette operation identify the cause and eliminate the fault To do this foll
6. 8 5000 ul tips bulk pack K943 10000 ul tips bulk pack P3985 Carousel stand for 6 pipettes P3989 Linear stand for 6 pipettes BioPette 12 The spare parts indicated Fig 3 4 5 and 6 that is Pipetting pushbutton A1 Pushbutton A2 Knob Adjustment knob Shaft Ejector Shaft nut Piston assembly Spacer O ring Seal Calibration key Filter Ejector cap Ejector pushbutton Locking ring can be ordered from your Labnet representative type of pipette and name of the part for this pipette should be specified YZST lt m O O D gt Warning The replacement of the piston requires con ducting of calibration procedure according to section 8 Before returning a pipette to a Labnet representative please ensure that the pipette is completely free of any contamination chemical microbial or radioactive Contact Labnet at 1 732 417 0700 fax 1 732 417 1750 email labnet labnetlink com All rights reserved Product described in this manual is subject to availa bility and technical modification Errors excepted PZ HTL S A reserves the right to improve enhance or otherwise modify its products without prior notification 2010 PZ HTL S A 15
7. It can measure and transfer volumes from 0 1 to 10000 ul depending on the model The BIOPETTE PLUS pipettes are equipped with a digi tal counter which shows the pipetting volume The set volume is visible in the window on the handle The setting of the volume is done by turning the pipetting pushbutton knob Fig 1A2 or by turning the adjustment knob Fig 1B in the correct direction The pipette design allows the user to lock the volume setting by pushing the locking ring upwards Fig 1S The position of the ring is indicated by the symbols located on the handle The volume range of the aspired liquid is shown on the pipetting pushbutton Fig 1 1 The BIOPETTE PLUS pipettes are produced in 8 models covering the volume range from 0 1 ul to 10000 ul 1 Model Volume range ul BPP2 0 1 2 BPP10 0 5 10 BPP20 2 20 BPP100 10 100 200 20 200 1000 100 1000 5000 1000 5000 10000 1000 10000 Measurement and transfer of micro volumes BPP2 BPP10 DNA sequencing and enzyme assay applications BPP20 BPP100 Measurement and transfer of general BPP200 BPP1000 aqueous solution acids and bases BPP5000 BPP10000 Measurement and transfer of large volumes The liquid is aspirated into disposable tips attached to the pipette shaft Disposable tips ensure maximum safety and eliminate possibility of crosscontamination of the liquid samples To protect the user from
8. OLUME The volume display shown by the counter has three dig its which should be read from top to bottom In addition the lowest counter drum is printed in a scale which enables the setting of the volume in the minimum gradua tion range Examples of the meanings of the black and red digits BPP2 Pipettes BPP2 red Red figures at the bottom 1 100 ul red Increment 0 002 ul 1 25 ul BPP10 BPP20 Pipettes BPP10 BPP20 Red figures at the bottom 1 10 ul red Increment 0 02 ul 7 5 yl 12 50 BPP100 BPP200 Pipettes BBPP100 BPP200 Black figures only 1 5 5 Increment 0 2 ul 75 yl 125 ul BPP1000 BPP5000 red 7 2 Pipettes BPP1000 BPP5000 5 5 Red figures at the top ml 0 75 ml 125m Increment 2 ul 10 ul BPP10000 Pipettes BPP10000 ie Red figures at the top ml Increment 20 ul 75 ml The volume of the pipette is set by the knob in the pipet ting pushbutton Fig 1A2 or by the adjustment knob Fig 1B The volume adjustment can be performed when the locking ring is set in the lower position fig 5A When the desired volume is selected the the locking ring should be set in the upper position Fig 5B To attain the maximum accuracy set volume must be approached from a higher value by diminishing counter readings f the desired volume is lower than set volume shown by the counter the operator should turn the pipetting pushbutton Fig 1A2 or the
9. ater and dry thor oughly Lightly lubricate the inside surfaces of the seal and the O ring with the lubricant Reassemble the pipette in the reverse order BioPette If you find an increase in the pipetting force which may happen after repetitive autoclaving of the pipette Remove the tip ejector Unscrew the shaft nut and then remove the shaft piston assembly seal and O ring Rinse the removed parts in distilled water and dry Lubricate the internal surfaces of the seal and O ring with lubricant that has been included with each pipette Reassemble the pipette in opposite order Note All parts of the pipette can be autoclaved see Section 10 The shaft of the 5000 and 10000 models should be autoclaved without the filter If the problem continues after carrying out the above steps contact your Labnet representative Before returning the pipette please ensure that the pipette is completely free of any chemical radioactive or microbiological contamination which could pose a threat during transport and repair As far as it is possible clean the pipette 10 CLEANING AND STERILIZATION Cleaning External surfaces of the pipetting pushbutton the ejector pushbutton the handgrip the shaft nut and the adjust ment knob may be cleaned using a cloth dampened in isopropyl alcohol The remaining parts removed from the pipette during pipette disassembly may be washed with distilled water or isopropyl alcohol Sterilization Th
10. contaminated tips the BIOPETTE PLUS is equipped with a built in tip ejector Fig 1D The construction of the ejector enables the user to adjust the length This allows for proper fit and ejection of nearly every tip type available When using narrow tubes it may be necessary to remove the tip ejector It is simply removed by pulling down Adjusting of the Tip Ejector Length in 2 1000 ul pipettes Fig 6A The adjusting H spacers included in the box allow for adjusting the length of tip ejector by 1 or 2 An H0 spacer is inserted on a standard basis The out side shape of the spacer identifies the size change in 5000 and 10000 ul pipettes Fig 6B The length of tip ejector is adjusted by screwing in or screwing out the tip ejector stem with a screwdriver Turn the screwdriver counter clockwise to increase the length of tip ejector and clockwise to reduce the length of tip ejector The ejector can be adjusted in the range of 5 mm If above described method of ejector adjustment is not sufficient or the diameter of the ejector opening is too large to eject the tip it is necessary to put the ejector cap M onto the ejector Fig 6C The BIOPETTE PLUS is a high quality instrument which offers excellent accuracy and precision BioPette The accuracy and precision repeatability of liquid vol ume depend on the quality of tips used The values for accuracy and precision given in the table below were ob
11. d within one full turn of the key to the right or to the left only Recalibration conditions Ambient temperature and the temperature of the pipet te tips and liquid should be within the range 20 25 and stabilized during weighing within 0 5 C Measurements should be conducted using distilled water Balance sensitivity should be suitable for the volume to be controlled Recalibration procedure Set the dose volume depending on the pipette volume according to the following table Volume change Range Preset Permissible AV for full turn of the pipette cc Model volume volumes of the calibration volumes ul ul ul key ul 24 increments BPP2 041 2 0 2 0 176 0 224 0 06 BPP10 0 5 10 0 5 0 48 0 52 0 33 BPP20 2 20 2 1 94 2 06 0 63 BPP100 10 100 10 9 84 10 16 2 50 BPP200 20 200 20 19 76 20 24 6 30 BPP1000 100 1000 100 98 4 101 6 25 00 BPP5000 1000 5000 1000 994 1006 125 00 BPP10000 1000 10000 1000 975 1025 250 00 Perform 5 aspirations weigh each one and calculate the average value of the aspirations Calculate average aspirated volume in ul multiplying the average aspiration amount mg by the distilled water density coefficient ul mg which depends on temperature and pressure according to the following table Pressure kPa Temperature 95 0 101 3 105 0 20 1 0028 1 0029 1 0029 21 1 0030 1 0031 1 0031 22 1
12. e pipette can be sterilized in an autoclave at 121 C for 20 minutes After sterilization the pipette should be dried and cooled to room temperature 18 It is recommended to sterilize the pipettes in autoclave with an initial vacu um and drying cycle prior to sterilization unscrew the shaft nut slightly in the BPP2 BPP1000 pipettes and unscrew the shaft slightly in the BPP5000 and BPP10000 After autoclaving these parts should be screwed tight again to set the locking ring in lower unlocked position prior to sterilization The precision of the results should not alter if the pipet ting process and autoclaving are carried out as described in this manual Because a slight change in the accuracy of the dosage may occur it is recommended to check the calibration of the pipette after the initial first third and fifth autoclaving cycles and then after every 10 autoclaving cycles Pipette kit The pipettes are delivered in the kits including Pipette Instruction manual Calibration key Shelf Clip Ejector regulation spacers for pipette models BPP2 BPP1000 Ejector cap for pipette models BPP5000 BPP10000 Identification labels Filters for pipette models BPP5000 BPP10000 Lubricant The shelf clip assembly diagram is shown in Fig 7 Accessories Cat No Description P1000 10 R 10 tips racked P1000 200 R 200 ul tips racked P1000 1000 R 1000 ul tips racked P399
13. hen aspirating and dispensing large volumes of liquid If the filter becomes wet during liquid aspiration it should be replaced with a new one 4 PRE RINSING When pipetting liquids of higher viscosity or lower sur face tension than water e g sera or organic solvents a film of liquid is formed on the inside wall of the pipette tip This film can create an error Since the film remains relatively constant in successive pipetting operations with the same tip this error can be avoided by forming the film before transferring the first sample This is done by aspirating a sample and dispensing it back into the same vessel Since the film is already formed all of the following samples will have better accuracy and repeatability This pre rinsing operation should be repeated when the volume to be aspirated is changed or when a new tip is used BioPette 5 DENSE AND VISCOUS LIQUIDS The BIOPETTE PLUS specifications of accuracy and pre cision are based on pipetting distilled water The handling of liquids with physical qualities of density viscosity and surface tension differing extremely from water may need a gravimetrically checked compensation of the volume setting Normally the degree of error resulting from heavy or viscous liquids is negligible if the pipetting is done slowly and carefully It is most important to give the liquids some time to follow the change of pressure by holding the pipette tip in its position fo
14. ow the instructions in the sequence provided Replacement of parts should be required only occasionally and should not occur under normal pipette use Droplets of liquid remain in the pipette tip The tip is emptied too fast Decrease the speed of pressing the pipette push button The tip wettability has increased due to extensive use Replace the tip with a new one Droplets of air appear in the liquid aspirated into the tip The pipette tip immersion is too shallow Immerse the tip deeper according to the instructions The pipette tip is incorrectly pressed onto the pipette shaft Press the pipette firmly The tip is damaged or worn out due to extensive use Replace the tip with a new one The pipette incorrectly aspirates the liquid or liquid drops out from the tip The pipette tip is incorrectly pressed onto the pipette shaft Press the pipette tip firmly The shaft nut is loose Fig in the models BPP2 BPP1000 14 Tighten the shaft nut The sealing surface of the shaft is cracked or scored Remove the tip ejector Unscrew the shaft nut inspect the shaft and the piston assembly Replace the damaged parts see Section 12 When reasembling the pipette the nut should be hand tightened In the models BPP2 BPP10 and BPP20 the damage of the shaft may also cause a damage of the piston assembly Replace the damaged parts see Section 12 When reasembling the pipette the nut should be hand tightened
15. petting acids or corrosive solutions which emit vapors it is recommended to disassemble the shaft and to rinse the piston and seal with distilled water after finishing the pipetting operation 8 RECALIBRATION Labnet pipettes are calibrated by gravimetric method using Labnet tips and distilled water at the temperature 20 1 C according to EN ISO 8655 standard If during pipette operation you find that the accuracy error the difference between the real aspirated volume and the preset volume exceeds the permissible value given in the table in section 1 the pipette recalibration procedure should be carried out Before starting the recalibration it is necessary to check whether the following requirements have been fulfilled during error determination the ambient temperature and the temperature of the pipette tips and water was identical the density of the liquid used is close to that of distilled water a balance with appropriate sensitivity has been used Volume checked ul Balance sensitivity mg 0 1 10 lt 0 001 10 100 s 0 01 gt 100 0 1 mg ul conversion factor has been taken into account the requirements given in sections 3 and 7 have been fulfilled If the above conditions are satisfied and the accuracy error for selected volume given in section 1 exceeds the permissible value the pipette recalibration procedure should be carried out The recalibration can be performe
16. r at least 2 sec after the aspiration and the blow out stroke If in extreme cases this method of operation does not result in accurate values a compensation could be achieved as follows Weigh the liquid pipetted when the BIOPETTE PLUS is set to the nominal value Then calculate the offset from the nominal value m Corr val 2x val m weight of the sample Y density of liquid Check this operation once again and correct if neces sary Note the corrected value for further pipetting the same kind of liquid 6 LABNET PIPETTE TIPS Labnet tips are made from high performance polypropy lene and their quality guarantees the precision and accu racy associated with the BIOPETTE PLUS Strict control is maintained throughout the manufacturing process to ensure the highest quality The accuracy and precision figures for the BIOPETTE PLUS are only guaranteed when Labnet tips are used The use of inferior quality tips will seriously degrade the performance of the BIOPETTE PLUS Tips 10 These tips are used for volumes between 0 1 ul and 10 ul They are used with the BPP2 and the BPP10 models Tips 200 These tips are used for volumes between 2 ul and 200 ul They are used with the BPP20 BPP100 and the BPP200 models which are equipped with the yellow label on the pushbutton 7 Tips 1000 These tips are used for volumes between 100 ul and 1000 They are used with the BPP1000 models which are equipped
17. s on the model Model Immersion depth mm BPP2 lt 1 BPP10 lt 1 BPP20 BPP100 2 BPP200 BPP1000 2 BPP5000 3 5 BPP10000 Release the pushbutton slowly and smoothly to aspirate the sample Fig 2B Wait one second and then with draw the tip from the liquid When the pipette tip is immersed not as deeply as the recommended depth or when the pipetting pushbutton is rapidly released air may enter the disposable tip 5 Avoid touching the orifice of the tip Dispensing Place the end of the tip against the inside wall of the vessel at an angle of 10 to 40 degrees Press the pushbutton smoothly to the first stop Fig 2C Wait one second Press the pushbutton to the second stop to expel any remaining liquid Fig 2D Keeping the pushbutton depressed to the very end remove the pipette by drawing the tip against the inside surface of the receiving vessel Release the pushbutton to its starting position Fig 2E Eject the tip by pressing the tip ejector button Fig 2F Remember to change the tip whenever a different liquid is to be sampled or the volume setting is changed Filters A replaceable filter installed in a seat in the bottom part of the shaft is used in 5000 ul and 10000 ul pipettes Fig 3L The filter prevents the aspirated liquid from entering into the shaft and thus from polluting the inside of the shaft and the piston Using the filter is especially important w
18. tained using Labnet tips Only those tips guarantee correct operation of the pipettes and ensure accuracy and precision of liquid sampling Accuracy Precision Fit to tips 0 2 19 0 6 0 2 1 0 x27 1 3 2 0 1 5 0 7 10 Min 0 5 4 0 28 BPP10 5 0 x0 0 6 10 0 0 5 0 4 Min 2 3 0 1 5 20 10 210 0 5 20 0 8 0 3 Min 10 1 6 0 80 100 50 0 8 0 24 200 100 0 8 0 20 Min 20 T2 0 60 BPP200 100 0 8 0 25 Max 200 0 6 0 20 Min 100 1 6 0 40 1000 500 0 7 0 20 1000 1000 0 6 0 15 Min 1000 0 6 0 25 5000 2500 0 6 0 20 5000 5000 0 5 0 15 Min 1000 2 5 0 6 10000 5000 0 8 0 3 10000 10000 0 5 0 2 These specifications are obtained in forward mode using a gravimetric method with the temperature of the distilled water tips and all other conditions stabilized between 19 and 21 C Number of measurements minimum 10 The values given include all components of error due to both normal handwarming and the changing of the tip Performance test The pipette is calibrated in accordance with EN ISO 8655 Performance can be verified by checking the pipette using the procedures outlined in the EN ISO 8655 standard The pipette design enables the user to perform the recalibra tion process according to the rules presented in section 8 2 SETTING THE V
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