User Manual
Contents
1. t O 1 f 2 f 8 f Repetitive pulses 0 1 9 9 milliseconds in duration with 0 1 ms of resolution are generated on Output The repetition cycle time Tc can be set from 10 ms 60 sec in 10 ms steps Synchron pulses are generated at the beginning of every Output pulses Continual Pulses function is a special case of Gated Continual function when BioStim Controller ignores the Gate signal resulting continuous flow of pulses beginning at START event Gated Continual START event H a E L GATE Input t Tx Ty OUTPUT Uout i O SYNCHRON Output Repetitive pulses 0 1 9 9 milliseconds in duration with 0 1 ms of resolution are generated on Output The repetition cycle time Tc can be set from 10 ms 60 sec in 10 ms steps Synchron pulses are generated at the beginning of every Output pulses The flow of Output pulses are disabled if TTL low level is applied to the Gate input The pulses are never broken because an asynchronous Gate signal is synchronised internally Remotely controlled bursts can be realised in this function using another stimulator controller or a computer Continual Pulses function is a special case of Gated Continual function when BioStim Controller ignores the Gate signal resulting continuous flow of pulses beginning at START event Continual Bursts START event H a tl L OUTPUT Uout O SYNCHRON Output t 0 1 f Repetitive bursts of pulses 2 99 pulses cycle are generate2. F button is used to cancel a function if it is running If you cancel a function with F button you will get an access to Function Choice menu START button is used to start the sequence of pulses if a single or a delayed function is selected actually The repetitive functions can be interrupted temporarily with START button and they can be restarted again with it The operating modes of the Repetition Cycle and Gate Controller in BioStim Controller equipment Repetition Cycle This function can be used to generate periodical repetitive trigger events in such cases when the BioStim Controller is the master synchron generator of the data acquisition process in such cases if the external data acquisition equipment such as a PC is triggered from its Synchron Output In this function the Synchron output of the Repetition Cycle and Gate Controller provides repetitive Start events for the Pulse Pattern Generator on which any of the single patterns should be selected To use the Repetition Cycle function the Synchron Output of the Repetition Cycle and Gate Controller should be connected to the Start input of the Pulse Pattern Generator with the appropriate switch at the back side of the BioStim Controller The repetition cycle time Tc can be set from 80 ms 60 sec with 10 ms of resolution A wide 50 ms Synchron pulse is generated at the beginning of every Repetition Cycles These Synchron pulses are the synchron pulses of
3. the whole time sequence if the Repetition Cycle function is used and a single pattern is initiated on the Pulse Pattern Generator The Repetition Cycle and Gate Controller has got its own TTL compatible Output which is independent from the Output of the Pulse Pattern Generator The Output pulse of the Repetition Cycle and Gate Controller 0 1 24 9 milliseconds in width with 0 1 ms of resolution can be used for any other controlling task for instance muting the input of the preamplifier for a programmed period before appearing and during the stimulating pulse at the Output of the End stage if it would be necessary Gate Generator This function can be used to generate a programmed enabling period for the Gated Continual function of the Pulse Pattern Generator To use the Gate Generator function the TTL compatible Output of the Repetition Cycle and Gate Controller should be connected to the Gate Input of the Pulse Pattern Generator with an external cable at the back side of the BioStim Controller This combination of functions can be used to produce very long bursts of pulses The duration of the burst is defined by the Repetition Cycle and Gate Controller The timing data of the pulses and the pauses should be programmed at the Pulse Pattern Generator in its Gated Continual function The gate time Tg can be set from 80 ms 60 sec with 10 ms of resolution A wide 50 ms Synchron pulse is generated at the beginning of every Gate pe
4. BioStim BioStim Controller amp Computer Interface User s Manual SUPE LECH The parts of the BioStim universal biological stimulator system The BioStim system is divided into two functional parts The time parameters are generated by the fully digital BioStim Controller and the analogue voltage and current sources are implemented in the End stages The parts of the system are manufactured as independent equipments On one hand it results a more flexible and variable system because the similar parts e g the End stages are compatible and they can be changed easily On the other hand the divided system meets the special experimental requirements much better because the analogue End stages can be located close to the biological objects in the shielded environment but the digital BioStim Controller can be placed anywhere else BioStim Computer Interface is an alternate of the BioStim Controller in special cases You can find detailed descriptions about each elements of the BioStim system in the appropriate manuals BioStim Controller BioStim Controller is a dual processor system There are two independent RISC microcontrollers built into one cage One of them the Repetition Cycle and Gate Controller can be used to generate the periodical repetition trigger events or it can be used as a gate generator to enable or disable the output pulse sequence The other microcontroller is the Pulse Pattern Generator It is used to generate the d
5. If you modify the pulse width the actual pulse will be finished with the last duration but the next one will be produced with the new duration at the next START event
6. ction will be started automatically BioStim Computer Interface There is an alternate equipment BioStim Computer Interface in our choice to substitute the BioStim Controller in that cases when a computer software is used to generate the sequence of the stimulating pulses BioStim Computer Interface has got a universal connector to connect any of End stages of Supertech a power supply unit to supply the End stage and two TTL compatible digital input connectors Its TTL inputs are configured in logical OR function internally The TTL standard describes the voltage and load specifications of the inputs and outputs of a logic system what is supplied from 5V Nowadays most of the microcontrollers and logic systems use 3 3V of supply voltage internally The inputs of the BioStim Computer Interface accept the logic levels of the 3 3V non standard TTL systems correctly with optimal noise margins too Basically BioStim Computer Interface realizes the DC via Control function see later in this description of BioStim Controller If a sophisticated software is used it is not necessary to install the BioStim Controller it is enough to use the BioStim Computer Interface The disadvantage of this arrangement is that a fast computer and an appropriate software is always necessary to be used An independent universal stand alone stimulator can be formed only by using of the BioStim Controller Programming conventions and definition of terms in conn
7. d on the Output The time parameters of the components in the bursts are the same as in the Single Burst and the Delayed Burst functions The repetition cycle time Tc can be set from 10 ms 60 sec with 10 ms of resolution A Synchron pulse is generated at the rising edge of the first Output pulse once at the beginning in every bursts Flexible Burst START event H a E L OUTPUT U I out O Ttotal SYNCHRON Output A freely defined burst of pulses can be composed in this function The number of pulses in the burst can be set from 2 to 10 The length of the pauses and the durations of the pulses can be set independently from each other The pauses can be programmed from 0 1 ms to 500 0 ms with 0 1 ms of resolution The width of pulses can be programmed from 0 1 ms to 25 0 ms with 0 1 ms of resolution A Synchron pulse 50 us is generated at START event at the beginning of the first pause in other words at the beginning of delay time period Variable Pulse START event H 7 tt L OUTPUT adjustable width SYNCHRON Output A delay 1 250 milliseconds in width with 1 ms of resolution is occurring after START event A Synchron pulse is generated at the beginning of delay time period Just after the delay time an Output pulse is appearing The duration of the Output pulse can be set from 10 ws to 20 0 ms with very fine 10 ws of resolution The width of the Output pulse can be modified on the fly
8. ection with the BioStim Controller The functions are categorized in two groups The first group contains the single functions Single functions are initiated by the START event After a START event they generate their sequence one time only an example is Single Burst The other category is the group of the repetitive functions They repeat their sequence periodically based on an internal crystal pacer an example is Continual Bursts A subcategory of the single functions is the group of the delayed functions They are the same as their appropriate single equivalents but a programmable delay is occurring at the beginning of the sequence before the first Output pulse START event means a keypress on START button or a TTL rising edge appearing on Start input The two source of Start event can be used together or independently any time they are in logical OR relation You can clear all stored parameters if you press and hold F button down while BioStim Controller is switched on During programming actions the equipment checks the validity of the actual value If you try to accept with F button a number out of range the software will not allow it If more than one parameters are incoherent you will be asked to correct them UP and DOWN button is used to navigate in menus and to modify parameter values up or down respectively F button is used to select a menu item or accept a parameter value Furthermore
9. ifferent output pulse sequences as listed below The accuracy of the time parameters in BioStim Controller are guaranteed by internal crystal pacers All the programmed time parameters are stored in built in nonvolatile memories They hold the previously used values during switched off periods Easy programming operations are carried out in menu system with 4 button keypads The displays of the microcontrollers are 4 x 20 and 2 x 16 character alphanumeric models with backlight to provide good visibility BioStim Controller together with one of the End stages can be used as a stand alone stimulator but both of its microcontrollers have got bi directional digital control capabilities Start Input Gate Input and Synchron Output These TTL compatible control bits offer a huge versatility in the different applications BioStim Controller can be started externally with rising edges at Start Inputs from another equipment for instance a PC or it can be the master synchron generator if the external equipments are triggered from its Synchron Outputs as well BioStim Controller has got nonvolatile memories to store all parameters of the functions If you use the equipment in a fixed application you should program it one time only If you switch the BioStim Controller on it checks which function was used last time After it the parameters used by the actual function are checked If the parameters have got valid values preset the last used fun
10. ith 0 1 ms of resolution A Synchron pulse 50 ws is generated at the START event at the beginning of the delay time period Single Pulse function is a special case of Delayed Pulse function when delay time equals to zero Single Burst START event H a tl L OUTPUT Uout O SYNCHRON Output A programmed number 2 99 pieces of pulses are generated at the START event The width of the pulses and the pauses between the pulses can be set in the range of 0 1 9 9 milliseconds with 0 1 ms of resolution A Synchron pulse 50 ps is generated at the START event once at the beginning of the first Output pulse in every bursts Single Burst function is a special case of Delayed Burst function when delay time equals to zero Delayed Burst START event H a tt L OUTPUT Uout j O SYNCHRON Output A delay 1 250 milliseconds in width with 1 ms of resolution is occurring after START event Just after the delay time a programmed number 2 99 pieces of pulses are generated The width of the pulses and the pauses between the pulses can be set in the range of 0 1 9 9 milliseconds with 0 1 ms of resolution A Synchron pulse 50 us is generated at the START event once at the beginning of the delay time period in every bursts Single Burst function is a special case of Delayed Burst function when delay time equals to zero Continual Pulses START event H oS L OUTPUT Uout i O SYNCHRON Output i t
11. nput i t OUTPUT Uout j O t 0 Output is active while START button is pressed or TTL high level is applied to START Input This mode offers free control capability from any other equipment e g another stimulator controller or a TTL port bit of a computer DC by Long Timer START event H 7 tt L OUTPUT Uout O t 0 Ttim Output is active constant DC level adjustable by the helical potmeter on the End stage in a programmed period in the range of 10 600 sec with 10 sec of resolution This period starts at the beginning of the START event Wide Pulse Pause START event H e tl L OUTPUT Uout i O t 0 Tp 2Tp 5Tp Wide pulses and pauses they are equal in width are generated The duration of the pulses and the pauses can be programmed from 10 990 milliseconds in 10 ms steps Single Pulse START event H i ee L OUTPUT Uout i O SYNCHRON Output One Output pulse is generated 0 1 9 9 milliseconds in width with 0 1 ms of resolution at the START event A Synchron pulse 50 ws is generated at the beginning of the Output pulse Single Pulse function is a special case of Delayed Pulse function when delay time equals to zero Delayed Pulse START event H Lt L OUTPUT t O SYNCHRON Output A delay 1 250 milliseconds in width with 1 ms of resolution is occurring after START event Just after the delay time an Output pulse is generated 0 1 9 9 milliseconds in width w
12. riods These synchron pulses are accessible at the Synchron Output of the Repetition Cycle and Gate Controller The operating modes of the Pulse Pattern Generator in BioStim Controller equipment The actually realized 12 operating modes are presented in the Appendix They have got a graphical interpretation to explain them in fine details In a few cases there are more than one function with which a paradigm can be carried out You should always consider which function is the best one to your special task Further development Until now we have developed many different operating modes for BioStim Controller as it is listed above In spite of this if you can not find the appropriate function for your special task in our actual choice and this function seems to be interesting for other our customers we will develop a special operating mode especially for you It is our method how we improve the features of our equipment We collect all the notices and feedbacks of our customers and we implement their may be your knowledge into the features of BioStim Warranty We give you full warranty service including rest parts for the period of 3 years by default Longer warranty periods can also be defined and agreed the actual conditions should be discussed before placing the order International technical hotline by phone 36 20 9234 386 Technical hotline by email csaba niedetzky superte ch DC via Control START I
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