PS300B - Hoefer Inc
Contents
1. disegnato conforme mente all EN61010 1 2001 la norma di sicurezza elettrica Tuttavia dovrebbe essere usato degli opera tori solo correttamente addestrati Leggere questo manuale intero prima di usare lo strumento e l uso solo secondo le istruzioni Lo strumento deve essere sempre usato col piombo di terra della spina di alimentazione correttamente hanno messo a terra alla terra alla presa di corrente principale Usa il filo metallico e Vapparecchiatura solo intatti elettrici specifici per i voltaggi che lei usera Tutta Vapparecchiatura collegata all alto voltaggio dovrebbe essere conformemente a EN61010 1 2001 lt Tiene lo strumento come secco e pulito come possibile Pulire regolarmente con un morbido per spegnere il panno Lasciare lo strumento asciuga completamente prima dell uso Non opera lo strumento nell umidita estrema al di sopra di 80 Evitare la condensazione lasciando o piv l unit equilibra alla temperatura ambiente quando portare lo strumento da un piu freddo a un ambiente piu caldo Di permettere raffreddare sufficiente assicura che gli sbocchi dello strumento non sono coperti Viktig Informasjon Norwegian lt Hvis dette utstyret blir brukt i en mate ikke spesifisert ved Hoefer Inc beskyttelsen som ha blitt git av utsty ret kan bli svekket Dette instrumentet er utformet for innendors labora toriumbruk bare Bare tilbehor og deler godkjente eller forsynte ved Hoefer Inc2. manual PS300B Power Supply um PS300B IM Rev C0 07 12 AAtioete r Page Finder Important Informatora teased AE ii Waste Electrical and Electronic Equipment WEEE ee vii Important user Information viii Power supply function and description 1 Unpacking ussi suvussaan a asa ea Para k 3 Specification currada eege ge det A Parameter Vi Wisin 5 Power supply operation sasar sasana akasa E aa an dk ga 7 CONNECTIONS sn Z RA A AE AEK Ata 8 Operati OMG ass aa aa Aa ENE acia 9 Power Failure Detection PFD 12 ll ee elle 13 Replacing the fuses AAA 14 Care and maintenance 444444142421111 15 Technical service and repair een 15 Ordering Information 15 Important Information English If this equipment is used in a manner not specified by Hoefer Inc the protection provided by the equipment may be impaired lt This instrument is designed for indoor laboratory use only lt Only accessories and parts approved or supplied by Hoefer Inc may be used for operating maintaining and servicing this product Warning Because this instrument can develop suffi cient voltage and current to produce a lethal shock care must be exercised in its operation This instrument is designed in accordance with the EN61010 1 2001 electrical safety standard Never theless it should be used only by properly trained operators Read this entire man
3. Inc reserves the right to make changes in the specifica tions without prior notice o pviii Important user information All users must read this entire manual to fully under stand the safe use of the PS300B WARNING The warning symbol highlights instructions that must be followed to avoid personal injury It is important not to continue until all stated conditions are met and clearly understood Read the instructions to avoid hazardous conditions CAUTION The caution symbol high lights instructions that must be followed to avoid damage to the product or other equipment It is important not to continue until all stated conditions are met and clearly understood Note Note is used to indicate information important for trouble free and optimal use of the product CE certification This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is e used in a laboratory location and e used as a stand alone unit or e connected to other CE marked Hoefer Inc instruments or e connected to other products recommended or described in this manual and e used in the same state as it was delivered from Hoefer Inc except for alterations described in this manual Note The four pairs of output jacks always operate at the same voltage
4. The sum of the current of all four outputs is displayed This value can never exceed 500 mA Power supply function and description The Hoefer PS300B power supply can be used with many electrophoresis applications such as running standard PAGE gels multiple PAGE minigels horizontal agarose gels semidry trans fer and minigel tank transfers The LED display reports power supply status Parameters are set by pressing UP DOWN arrow buttons in combi nation with a SELECT button Four pairs of output jacks accept 4 mm plugs which operate in parallel The output jacks are color coded recessed for safety and accept 4 mm shielded banana plugs that come standard with most Hoefer equipment The user interface consists of a three digit LED readout and five buttons on a membrane keypad Diagrams of the front and rear panel are shown on the following pages The PS300B operates in constant voltage or constant current modes Power output is controlled by setting a maximum value for volt age up to 300 V or current up to 500 mA The maximum output power at any time is 90 Watts Run duration can be continuous or programmed to a maximum duration of 999 minutes Fig 1 Front view of PS300B Power Supply 3 digit display UP DOWN arrows SELECT display highlighted parameter CONSTANT V or A toggle START STOP mm polari
5. Todo equipo conectado al voltaje alto debe ser de acuerdo con EN61010 1 2001 Mantiene el instrumento tan seco y limpio como posible Enjugue regularmente con un suave el trapo h medo Permita que el instrumento seque completa mente antes de uso No opera el instrumento en la humedad extrema encima de 80 Evite condensaci n permitiendo la unidad equilibra a la temperatura ambiente al tomar el instrumento de un m s fr o a un ambiente m s tibio Permitir refrigeraci n suficiente asegure que las aberturas del Viktig Information Swedish lt om denna utrustning anv nds i ett s tt som inte har specificeras av Hoefer Inc skyddet tillhandah ll vid utrustningen kan skadas Detta instrument formges f r inomhuslaboratorium anv ndning bara Bara medhj lpare och delar godk nde eller levererade vid Hoefer Inc kan anv ndas f r fungera underh lla och servicing denna produkt varna D rf r att detta instrument kan utveckla tillr ck lig sp nning och str m att producera en d dlig st t m ste vas omsorg i dess funktion Detta instrument formges i verensst mmelse med EN61010 1 2001 elektriska s kerheten standarden Icke desto mindre b r det anv ndas bara av riktigt utbildade operat rer Lis denna hela handbok f re anv nda instrumentet och anv nd bara enligt under visningarna lt Instrumentet m ste alltid anv ndas med jorden blyet av kraften repet riktigt grounded till j
6. kan bli brukt for drive vedlikeholde og betjene dette produktet Varsler Fordi dette instrumentet kan utvikle tilstrek kelig spenning og str m til 4 produsere et d delig sjokk m bli vd bekymring i dets drift Dette instrumentet er utformet i samsvar med EN61010 1 2001 elektrisk sikkerhetsstandard Likevel burde bli brukt det bare av skikkelig utdannede oper at rer Les denne hele h ndboken f r bruking instru mentet og bruken bare gi til instruksjonene Instrumentet m alltid bli brukt med jorden blyet av kraftkabelen som riktig ha blitt jordet til jord p hovedledningen utl p lt Bruker bare uskadd elektrisk ledningsfremforing og utstyr som er spesifikk for spenningene du vil bruke All utstyr koplet til h yspenning burde v re i samsvar med EN61010 1 2001 Beholder instrumentet som t rker og rengjor som mulig Visk regul rt med et mykt fuktig stoff La instru mentet t rker komplett f r bruk lt Driver instrumentet i ekstrem fuktighet ikke ovenfor 80 Unng kondensasjon ved la enheten equili brate til omgivelsestemperatur ved taen instrumentets fra et kaldere til et varmere milj Til tillate tilstrekkelig kj lig sikrer at ventilasjon s pningene av instrumentet er ikke dekket Wazne Informacje Polish Je eli ten sprz t jest wykorzystywany w spos b nie okre lone przez Hoefer Inc do ochrony przewidzianej przez urz dzenie mo e zosta obni ony lt Instrument t
7. minen varmistaa ett v lineen ilmarei t peitet n Information Importante French Si cet quipement est utilis dans une mani re pas sp cifi par Hoefer Inc la protection fourni par V guipement pourrait tre diminu e Cet instrument est con u pour l usage de laboratoire int rieur seulement Seulement les accessoires et les parties ont approuv ou ont fourni par Hoefer Inc pourrait tre utilis pour fonctionner maintenir et entretenir ce produit lt Avertissant Parce que cet instrument peut d velopper la tension et le courant suffisants pour produire un choc mortel le soin doit tre exerc dans son op ration Cet instrument est conform ment con u a l EN61010 1 2001 norme de s curit lectrique N anmoins piii il devrait tre seulement utilis par les op rateurs convenablement entrain s Lire ce manuel entier avant utiliser l instrument et usage seulement selon les instructions lt Linstrument toujours doit tre utilis avec l avance de terre du cordon d alimentation correctement a fond a la terre a la sortie principale Utiliser le fil et l quipement lectriques seule ment intacts sp cifiques pour les tensions que vous utiliserez Tout 6guipement connect a haute tension devrait tre conform ment a EN61010 1 2001 lt Garder l instrument aussi sec et propre comme possi ble Essuyer r guli rement avec un doux touffer du tissu Laisser instrume
8. of the buffer during the run If the voltage never exceeds 180 V during the run then the gels will run at 60 mA for the entire run If the volt age increases to 180 V then the power supply will automatically crossover to constant voltage mode in order to keep voltage from exceeding 180 V Now output current will slowly decrease below the set limit of 60 mA because the 180 V set limit is now the limiting value in the circuit e pll e pl2 Power Failure Detection PFD The Power Failure Detection feature will save the time voltage and current settings in the event of a power outage and automatically continue the run when the power is restored To alert the user that a power failure occurred during the run the error message E6 flashes at the end of the run instead of the usual OFF message The E6 error message means that despite the power outage the power supply successfully supplied the programmed voltage and current for the entire length of time that was programmed The time shown at the end of the run is the time the gel ran since power was restored not the total time of the run The default settings for Power Failure Detection are Run Time Setting PFD Code Timed Run 1 999 minutes ON PF1 Continuous Run 0 OFF PFO The Power Failure Detection feature can be deactivated during a timed run 1 Press the SELECT button to move the indicator light under the Clock symbol 2 Press the UP and DOWN butt
9. the actual V or A output or the time press the SELECT button to toggle between the values In the case of a continuous run press START STOP to terminate the run The LED display reads OFF A timed run automatically stops with a warning beep The word OFF and the conditions at the end of the run are flashed on the display so that the user can record the end conditions Pushing the SELECT button will return the power supply to the last run settings Pressing the CONSTANT button will reset the power supply Example Set up conditions for running 2 minigels each at a constant current of 30 mA 1 Press the CONSTANT button to position the indicator light under A 2 Press the UP and DOWN buttons to set the LED display value 60 2 minigels x 30 mA each 3 Press the SELECT button to position the indicator light under V Observe that the volt limit has been automatically set to the maximum value of 300 V Setting the voltage limit to the maximum setting ensures that the entire separation is run at 60 mA for as long as possible Should the buffer or gel lose too much conductivity during the run the operational 300 V or 90 W limits may be reached at which point crossover automatically occurs and V becomes the limiting variable The current would slowly drop below 60 mA at this point Note If this crossover condi tion occurs and is not desired change the voltage or current setting to the po
10. acting both surfaces of the gel A constant volt run with low volt setting may result in a current below the 4 mA limit Press any button to clear the error code Check and correct all potential resistance problems Check buffer volumes Check all voltage leads Check the fit of power supply adapters if used The output current may have fallen below the 4 mA limit of the power supply Press any button to clear the error code Restart the run if desired Press any button to clear the error code Restart the run if desired Press any button to clear the error code See page 12 for information on the PFD option Press any button to clear the error code See page 12 for information on the PFD option Check for any short circuit or evidence of electrical arcing Buffer concentration too high A constant current run with low current may result in a voltage below the 10 V limit Press any button except the START STOP button to clear the Error Message Other Problems No display and or fan not operating Check that the power supply is plugged into a working receptacle Check that the main power switch is on Check fuses e pl3 CAUTION Turn the mains power supply switch off and detach the power cord before replacing input mains fuses e pl4 Replacing the fuses e Unplug the power cord from the power supply e Locate the fuse compartment on the rear panel e Use a screwdriver to pry out the fuse c
11. bruikt handhavend en onderhouden van dit product Waarschuwend Omdat dit instrument voldoende spanning en stroom kan ontwikkelen om een dodeli jke schok te produceren moet zorg in zijn operatie worden geoefend lt Dit instrument is in overeenstemming met de EN61010 1 2001 elektrische veiligheidsstandaard ontworpen Niettemin zou het enkel door goed getrainde bedieningslieden moeten worden gebruikt Lees dit volledige handboek voor het gebruik het instrument en gebruik enkel volgens de instructies Het instrument moet altijd met de aardeleiding van het stroomsnoer correct grondde naar aarde aan het hoofdafzetgebied worden gebruikt Gebruik enkel onbeschadigde elektrische draad en uitrustings specifiek voor de spanningen u zult gebruiken Alle uitrustingen sloten aan aan hoogspan ning zou in overeenstemming met EN61010 1 2001 moeten zijn Houd het instrument zo droge en schone zoals mogelijk Bij Wis regelmatig met een zacht temper doek Verhuur het instrument droogt volledig voor het gebruik lt Bedien niet het instrument in extreme vochtigheid bovenstaande 80 Vermijd condensatie door het verhuren van de eenheid in evenwicht brengt naar omgevingstemperatuur wanneer nemen het instru ment van een kouder naar een lievere omgeving lt Om toe te staan voldoende afkoelen verzeker dat de luchtopeningen van het instrument niet bedekt zijn T rke Tietoa Finnish Jos t t varusteita k ytet n tava
12. dbringende chok skal pleje bruges i dets drift Dette instrument er designet i overensstemmelse med EN61010 1 2001 elektrisk sikkerhedstandard Alligevel skulle det bruges bare af passende traenede operatorer Laes denne hel h ndbog for bruging instrumentet og brug bare i henhold til instruktionerne lt Instrumentet skal altid bruges med jordblyet af netledningen rigtigt jordede til jord p hovedledning sudlobet lt Bruger bare uskadt elektrisk trad og udstyr som v re specifik for spaendingerne du vil bruge Alt udstyr forbundet til hoj spaending skulle vaere i overensstem melse med EN61010 1 2001 lt Beholder instrumentet s tor og ren som mulig Tor regul rt med et bl dt fugtigt stof Lad instrument torken komplet for brug o pii lt Driver ikke instrumentet i yderst fugtighed ovenfor 80 Undg kondensation ved lade enheden equili brate til omgivende temperatur ved tageen instru mentets fra et koldere til et varmere miljo At tillade tilstr kkelig afk ling forsikrer at lufthullerne af instrumentet er ikke d kket Belangrijke Informatie Dutch lt Indien deze uitrusting in een manier wordt gebruikt die niet door Hoefer is gespecificeerd Nv de bescherming die door de uitrusting is verzorgd kan worden geschaad lt Dit instrument is voor binnenlaboratoriumgebruik enkel ontworpen Enkel onderdelen en delen keurden goed of leverden door Hoefer Nv kan voor het bedienen worden ge
13. de am Hauptausgang verwendet werden Nurunbesch digte elektrische Leitung und Ausr s tung spezifisch f r die Stromspannungen verwenden die Sie verwenden werden Die ganze mit der Hoch spannung verbundene Ausriistung sollte in Uberein stimmung mit EN61010 1 2001 sein Das Instrument ebenso trocken halten und reinigen wie m glich Wischen Sie regelm ig mit einem weichen befeuchten Sie Stoff Lassen Sie das Instru ment trocken v llig vor dem Gebrauch lt Das Instrument in der u ersten Feuchtigkeit ber 80 nicht bedienen Vermeiden Sie Kondensation die Einheit equilibrate zur Umgebungstemperatur laBend wenn Sie das Instrument von einem k lteren bis eine w rmere Umgebung nehmen Um das gen gend Abk hlen zu erlauben stellen Sie sicher dass die ffnungen des Instrumentes nicht bedeckt werden Informazioni Importanti Italiano Se quest apparecchiatura usata in un modo specificato da Hoefer Inc la protezione fornito dall apparecchiatura potrebbe essere indebolita Questo strumento disegnato per l uso di laboratorio interno solo Solo gli accessori e le parti hanno approvato o hanno fornito da Hoefer Inc potrebbe essere usato per oper are per mantenere e per revisionare questo prodotto Avvertendo Perch questo strumento pu sviluppare il voltaggio sufficiente e la corrente di produrre una scossa letale la cura deve essere esercitata nella sua operazione Questo strumento
14. en jest przeznaczony do u ytku w labora toriach kryty tylko Tylko akcesori w i cz ci zatwierdzone lub dostarczone przez Hoefer Inc mog by wykorzystane do eksp loatacji utrzymania i obs ugi tego produktu Uwaga Poniewa ten akt prawny mo e by rozwini cie odpowiednich napi cie i bie cych do wyprodu kowania miertelnego szoku opiek musi by wykony wane w dzia aniu lt Ten instrument zosta zaprojektowany zgodnie z tym EN61010 1 2001 Bezpiecze stwo elektryczne standard Niemniej jednak nale y stosowa jedynie przez odpowiednio przeszkoleni operator w Znajd pa stwo to ca y podr cznika przed zastosowaniem instrumentu i stosowa jedynie zgodnie z instrukcjami lt Instrument musi zawsze by wykorzystane z ziemi doprowadzi do zasilania detonuj cego w a ciwie uzasadnione na ziemi w sieci wodoci gowej rynku zbytu lt Wykorzystanie tylko nieuszkodzona elektrycznych drut w i urz dzenia specjalne do napi zap ac wykorzystania Wszystkie urz dzenia pod czone do wysokiego napi cia powinny by zgodne z EN61010 1 2001 Kontrolowa instrumentu jako suche i czyste jak to mo liwe Wytrze regularnie przy pomocy mi kkiego wilgotnej szmatki Niech si instrumentem ca kowicie wysuszy przed u yciem Nie prowadz do instrumentu w skrajnych wilgotno ci powy ej 80 Zapobiec kondensacji najmu przez jednostk r wnowa y do temperatury pokojowej przy podejmowa
15. ing safety tips will reduce the risk of electrical shock 1 Turn off the power supply before connecting or discon necting the voltage leads 2 Keep fingers away from metal components when connecting or disconnecting the voltage leads Fig 4 The output jacks are recessed and color coded e p8 Connections Connect the mains power cord Select the appropriate mains power cord for your region and connect it to the power cord receptacle on the rear panel and to a suitable grounded AC power outlet Place the power supply so that the fan on the back panel and the vents on the bottom are not obstructed e Connect the HV power leads Connect the apparatus to the power supply by plugging the lead connectors into one pair of the recessed output jacks Fig 4 Turn on the main power switch on the rear panel The front panel should be illuminated black jacks Note When changing timer between O and 1 a message PF1 or PFO will briefly be displayed indicating that the Power Failure Detection Feature is either on PF1 or off PFO See page 12 for more information on Power Failure Detection Operation 0 Select constant mode Press the CONSTANT button to toggle between constant voltage and constant current mode The selection is indicated by a green light e Adjust limit Press the UP and DOWN buttons to set the constant parameter to the desired value Check other r
16. k bezpe nostn normy P esto e by m ly b t pou ity pouze dn vy kolen oper tor st cel toto ru n p ed pou it m n stroje a pou it pouze v souladu s pokyny P stroj mus b t v dy pou vaj se na v konu zemi v st ra spr vn zemn ny k zemi na s ti v stce Vyu it pouze nepo kozen elektrick dr ty a vybaven pro nap t budete pou vat V echna za zen spojen s vysok m nap t m by m la b t v souladu s EN61010 1 2001 Si ponech n stroje jako such a ist jako mo n Ot ete pravideln s a m kk vlhk m had kem Nech je n stroj nenastaven pln p ed pou it m Nejsou provozov na na n stroj v extr mn vlhkost nad 80 P ede lo kondenzaci o pron jmu jednotky na okoln teplotu nech p i p ij m n n stroj z chladn j do tepl ho prost ed Pro umo n n dostate n chlazen zajistit aby otvory n stroje jsou nevztahuje Vigtig Information Danish Hvis dette udstyr bruges i en made ikke specificeret ved Hoefer Inc den beskyttelse som er blevet forsynet af udstyret kan maske svaekkes Dette instrument er designet for indendors laboratori umbrug bare Bare tilbeh r og del godkendede eller forsynede ved Hoefer Inc kan maske bruges for drive funktionsfejl og betjening dette produkt lt Advare Fordi dette instrument kan udvikle tilstraek kelig spaending og strom at fremstille et do
17. niu instrumentu z ch odniejsze w cieplejszych rodowiska Aby umo liwi wystarczaj ce ch odzenia zapewniaj e rozci cia of the instrument nie obj te ubezpieczeniem Informa es Importantes Portuguese Se este equipamento usado numa maneira n o espe cificada por Hoefer Inc que a protecc o fornecida pelo equipamento pode ser comprometida Este instrumento projectado para uso de interior de laborat rio s S acess rios e partes aprovaram ou forneceu por Hoefer Inc pode ser usada para operar manter e servicing este produto Advertindo Porque este instrumento pode desen volver voltagem suficiente e corrente produzir um choque letal cuidado deve ser exercitado em sua operac o lt Este instrumento projectado de acordo com o o pv EN61010 1 2001 condic o de seguranca el ctrica Nao obstante deve ser usado s por operadores adequada mente treinados Leia este manual inteiro antes de usar o instrumento e use s de acordo com as instrucc es lt Oinstrumento sempre deve ser usado com o chumbo de terra do cord o de poder corretamente baseou a terra nos canos saida principais Usa fio el ctrico s intacto e equipamento espec fico para as voltagens que voc usar Todo equipamento conectado a voltagem alta deve ser de acordo com EN61010 1 2001 Mantem o instrumento tao seco e limpo como poss vel Limpe regularmente com um pano h mido macio Deixe o instrumento seca
18. nt seche completement avant l usage Ne pas fonctionner l instrument dans l extr me humidit au dessus de 80 Eviter la condensa tion en laissant l equilibrate d unit la temp rature ambiante en prenant l instrument d un plus froid un environnement plus chaud lt Permettre le refroidissement suffisant garantir que les conduits de l instrument ne sont pas couverts Wichtige Informationen German Wenn diese Ausr stung gewisserma en nicht angege ben durch Hoefer Inc verwendet wird kann der durch die Ausr stung zur Verf gung gestellte Schutz verschlechtert werden lt Dieses Instrument wird f r den Innenlaborgebrauch nur daf r entworfen lt Nur Zus tze und Teile genehmigten oder lieferten durch Hoefer Inc kann fiir das Funktionieren das Aufrechterhalten und die Wartung dieses Produktes verwendet werden lt Die Warnung Weil dieses Instrument geniigend Strom spannung und Strom entwickeln kann um einen t dli chen Sto zu erzeugen muss Sorge in seiner Operation ausge bt werden lt Dieses Instrument wird in Ubereinstimmung mit dem EN61010 1 2001 elektrischen Sicherheitsstandard daf r entworfen Dennoch sollte es nur von richtig erzo genen Maschinenbedienern verwendet werden Lesen Sie dieses komplette Handbuch vor dem Verwenden des Instrumentes und verwenden Sie nur gemaB den Instruktionen lt Das Instrument muss immer mit der Erdleitung der Macht Schnur richtig niedergelegt zur Er
19. ompartment There are two fuses in the compartment Check and replace any blown fuses with a fuse of the same size and ratings e FUSES 2 250 V 3 15 A 5 x 20 mm fast blow Care and maintenance e Turn the mains power switch off and unplug the power cord before cleaning e Use a soft cloth dampened with water or a mild cleaning solution to clean the cabinet and display e If spilled liquids contact the circuit boards unplug the power supply and allow to dry completely Call Hoefer Inc for advice before using Technical service and repair Hoefer Inc offers complete technical support for all of our products If you have any ques tions about how to use this product or would like to arrange to repair it please call or fax your local Hoefer Inc representative Ordering information Qty Code no PS300B Power Supply 1 PS300B universal voltage Power Cord US 1 PSCORD 115V Power Cord Euro 1 PSCORD 230V User Manual 1 PS300B IM Fuse 250 V3 15AFB pack 5 PSF3A FB 5X20 5 x 20 mm e pl5 Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support amp hoeferinc com Web www hoeferinc com Hoefer is a registered trademark of Hoefer Inc O 2012 Hoefer Inc All rights reserved Printed in the USA Hoefer
20. ons to enter in the run time in minutes 3 Press both the UP and DOWN buttons at the same time The display will briefly show PFO indicat ing that the PFD mode is disabled 4 Press both the UP and DOWN buttons at the same time again The display will show PF1 indicat ing that the PFD mode is enabled Troubleshooting The power supply has built in error messages to help troubleshoot problems with the electrical circuit The error messages and possible corrective actions are listed below Error code El E3 E4 E5 E6 E7 Explanation No Load Detected The voltage leads to the electrophore sis cell are not attached The current load is below 4 mA the lower limit of the power supply Change in Load Resistance Open circuit was detected at some point after a successful start Buffer is leaking from the cells There is a loose electrical connection in the circuit Power Failure during a continuous run no timer being used Power Failure during a timed run with PFD option disabled Run was not completed Power Failure during a timed run with PFD option enabled Despite the power outage the run was completed successfully Short Circuit Load current exceeded 500 mA The voltage load is below 10 V the lower limit of the power supply Possible solutions Check all connections Verify that buffer volumes are sufficient to contact all electrode wires and that buffer is cont
21. orden p det huvudutloppet Anv nder bara undamaged elektrisk trad och utrust ning specifik f r sp nningarna du ska anv nda All utrustning kopplats som till h g spanning skulle vara i verensst mmelse med EN61010 1 2001 lt Haller instrumentet d torkar och reng r som m jlig Torka regelbundet med en mjuk fuktig trasa Lat instrumentet torka fullstandigt fore anvandningen lt Fungerar inte instrumentet i extrem fuktighet ver 80 Undvik kondensering vid lata enheten equili brate till omgivande temperatur nar ta instrumentet fran en kallare till en varmare milj Atttill ta tillr cklig kyla ser till att halen av instru mentet inte t cks o pvi English Ea French R German R Ea Italian R Esa Spanish La Swedish R Waste Electrical and Electronic Eguipment WEEE This symbol indicates that the waste of electrical and electronic eguipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your eguipment Ce symbole indigue que les d chets relatifs a guipement lectrique et lectronique ne doivent pas tre jet s comme les ordures m nag res non tri es et doivent tre collect s s par ment Contactez un repr sentant agr du fabricant pour obtenir des informations sur la mise au rebut de votre quipement Dieses S
22. r completamente antes de uso lt Nao opera o instrumento em humidade extrema acima de 80 Evite condensa o deixando o equili brate de unidade a temperatura ambiental quando tomar o instrumento de um mais frio a um ambiente mais quente lt Permitir esfriar suficiente assegura que as aberturas do instrumento n o s o cobertas Informaci n Importante Spanish Si este equipo es utilizado en una manera no especifi cado por Hoefer S a la protecci n proporcionado por el equipo puede ser da ada Este instrumento es dise ado para el uso interior del laboratorio s lo S lo accesorios y partes aprobaron o suministraron por Hoefer S a puede ser utilizado para Operar para mantener y para atender a este producto lt Advirtiendo Porque este instrumento puede desar rollar voltaje y corriente suficientes para producir un golpe mortal el cuidado debe ser ejercitado en su operaci n Este instrumento es dise ado de acuerdo con el EN61010 1 2001 est ndar el ctrico de seguridad No obstante debe ser utilizado s lo por operarios adecuadamente capacitados Lea este manual entero antes de utilizar el instrumento y el uso s lo seg n las instrucciones El instrumento siempre debe ser utilizado con el plomo de la tierra del cable de alimentaci n moli correcta mente a la tierra en la salida de red Utiliza alambre y equipo el ctricos s lo ilesos espec ficos para los voltajes que usted utilizar
23. ssa ei m ritetty Hoeferille Inc suojelu ehk isty varusteille saattaa olla avuton lt T m v line suunnitellaan sis laboratoriok yt lle vain Vain lis varusteet ja osat hyv ksyiv t tai toimitti Hoeferin oheen Inc voi k ytt k ytt miselle valvoalle ja servicing t m tuote Varoittaminen Koska t m v line voi kehitt riitt v j nnitteen ja virran tuottaa kuolettavan j rkytyksen huolta t ytyy harjoittaa toiminnossaan T m v line suunnitellaan EN61010 1 2001 s hk turvallisuusstandardin mukaisesti Silti pit isi k yt t vain ohi oikeasti koulutetut k ytt j t Lue t m kokonainen manuaalinen ennen v linett ja k ytt vain ohjeiden mukaan lt V linett t ytyy k ytt aina valtanuoran maalyijyst perusti oikein maadoittaa s hk verkkoaukossa K ytt vain undamaged s hk metallilankaa ja varus teita t sm llinen j nnitteille k ytt Kaikki varusteet yhdistetty korkeaan j nnitteeseen pit isi olla EN61010 1 2001IN mukaisesti Pit v lineen yht kuiva ja puhdas kuin mahdollinen Pyyhi s nn llisesti pehme ll kostealla kankaalla Anna v line kuivua t ysin ennen k ytt lt Ei k yt v linett extreme ilmankosteudessa 80 n yl puolella V lt tiivistymist antamalla yksik n equili brate ymp r iv n l mp tilaan kun ottaminen v line kylmempi l mpim mp n ymp rist n lt Sallia riitt v n j hdytt
24. t Crossover from constant A to constant V Voltage limit 000000000000 voltage Current limit AT current Fig 3 Power supply crossover resistance increasing over time Crossover from constant V to constant A Voltage limit 9 7 e voltage Current limit lt A current resistance decreasing over time There are some conditions when the power supply will operate at the upper limit of 90 W of constant power When this occurs both the constant V and the constant mA lights will be lit at the same time WARNING Use only one hand when making or breaking a connection to avoid making a complete circuit across your chest Also while connecting or disconnecting the leads do not allow any part of your body to contact a grounded surface Make sure your other hand is not touching anything that grounds you WARNING If you are in the middle of a run and wish to connect or disconnect the leads always turn the power supply off press START STOP Wait for the display to read OFF and the high voltage light to turn off Power supply operation Recommended leads 4 mm banana plugs fixed insulating sheaths which are found on most Hoefer equipment Will also accept e 4 mm banana plugs unsheathed or retractable sheaths e Stackable plugs Exposed metal surfaces on voltage leads can potentially lead to electrical shocks If using these types of voltage leads the follow
25. t limitations e The maximum power supply output is 90 W so the maxi mum current of 500 mA is available at 180 V or less e At the maximum voltage setting of 300 V the current is limited to 300 mA Note In most cases only one parameter can be held constant at a time Parameter overview Power parameters Because the power output limit is 90 W and W V x A the maximum current of 500 mA can be delivered only if voltage is lt 180 V Conversely the maximum voltage of 300 V can be delivered only if the current is lt 300 mA Programmed limits Variable Range Increment Voltage 10 300 V 1 volt Current 4 500 mA 1 milliamp Time 0 999 minutes 1 minute Timer O continuous run Timer 1 999 timed run in minutes Power supply output limits The PS300B can be programmed to limit voltage V or current mA The power supply will operate at the set limits provided the load or power requirement does not exceed the power supply rating Crossover occurs when the second parameter limit is reached as system resistance changes Fig 3 At this point the second parameter becomes constant and the previously unchang ing parameter adjusts downward as system resistance changes System resistance is affected by discontinuous buffers changes in tempera ture and buffer depletion When the power supply is delivering power the light above the CONSTANT button indicates which parameter is being held constan
26. ty Output jacks 1LL polarity e p2 Fig 2 Rear view of PS300B Power Supply Unpacking Unwrap all packages carefully and compare contents with the packing list making sure all items arrived If any part is missing contact your local Hoefer Inc sales office Inspect all components for damage that may have occurred while the unit was in transit If any part appears damaged contact the carrier immediately Be sure to keep all packing material for damage claims or to use should it become necessary to return the unit Fan Fuses Power Cord In ON OFF Switch e p3 e p4 Specifications Power requirement Frequency 50 60 Hz Line voltage 100 240 VAC 144 VA User interface 3 digit red LED membrane keypad Output 10 300 V 1 V increment 4 500 mA 1 mA increment 90 W maximum 4 pairs output jacks in parallel Output connector 4 mm plugs Timer 1 999 min 1 min increment Environmental operating Indoor use 4 40 C conditions Relative humidity 0 80 noncondensing Altitude lt 2000 m Pollution degree 2 Installation category Dimensions h x wx d 33 5 x 12 5 x 28 cm 13 2 x 4 9 x 11 0 in Weight 2 4 kg 5 3 Ib Safety protection circuits Short circuit protection Open circuit protection Product certifications EN61010 1 2001 EN61326 A3 2003 Class B CE WEEE RoHS Please note the following power outpu
27. ual before using the instrument and use only according to the instructions The instrument must always be used with the earth lead of the power cord correctly grounded to earth at the mains outlet Use only undamaged electrical wire and equipment specific for the voltages you will use All eguipment connected to high voltage should be in accordance with EN61010 1 2001 Keep the instrument as dry and clean as possible Wipe regularly with a soft damp cloth Let the instrument dry completely before use Do not operate the instrument in extreme humidity above 80 Avoid condensation by letting the unit equilibrate to ambient temperature when taking the instrument from a colder to a warmer environment To permit sufficient cooling ensure that the vents of the instrument are not covered Dule it Informace Czech Pokud by toto za zen je pou ito zp sobem kter neni podle Hoefer ochrana poskytovan na z klad Inc za zen m e b t naru ena lt Tento n stroj je ur en pro vnit n pou it v laborato i pouze Pouze p slu enstv a sti schv len nebo poskyt nut ch Hoefer Inc mohou b t pou ity pro provoz dr bu a dr b tohoto v robku Pozor Proto e tento n stroj m e vyvinout dostate n nap t a proud kter m vyr b t a smrtiacej ok p e mus b t vykon v na v jeho provoz lt Tento n stroj je ur en v souladu s EN61010 1 2001 elektric
28. un parameter Press the SELECT button to check the set value of the other non constant run parameter This parameter is set by default to the maximum output of the power supply This is the normal setting for many electrophoretic separations Optional If there is another upper limit desired for this non constant parameter use the UP and DOWN buttons to set it to the desired value The power supply can then operate in Crossover mode as explained on page 5 o Set the timer optional Press the SELECT button to move the green indicator light to under the clock symbol Continuous run Set the timer at O to start a contin uous run The power supply will operate indefinitely until stopped manually The timer will start counting up indicating the length of the run in minutes Timed run Press the UP and DOWN buttons to set the timer to the desired run time from 1 to 999 minutes The timer counts down during the run indicating the time remaining Note The power supply slowly increases to the set value when starting a run The constant parameter can be adjusted during a run e plo Start the run Press the START STOP button The green light above the START STOP button indicates that voltage and current are being applied to the output jacks The green light above the CONSTANT button indicates which parameter V or A is limiting and constant The LED display shows the numerical value of
29. wer supply s operational maximum 500 mA or 300 V 4 5 Press the SELECT button to position the indicator light under the Clock symbol If desired use the UP button to set the timer to a desired duration If the run is continuous set the timer value to 0 Press the START STOP button to start the run Example Set maximum mA 30 mA per gel and maxi mum V 180 V settings with automatic crossover The power supply can automatically crossover between two different maximum settings if desired This will be illustrated below 1 Press the CONSTANT button to position the indi cator light under A Press the UP and DOWN buttons to increase the LED display value to 60 2 minigels x 30 mA each Press the SELECT button to position the indicator light under V Observe that the volt limit has been automatically set to the maximum value of 300 V Press the UP and DOWN buttons to adjust this value down to 180 V Press the SELECT button to position the indicator light under the Clock symbol If desired use the UP button to set the timer to a desired duration If the run is continuous set the timer value to O Press the START STOP button to start the run Both Voltage and Current are monitored during the run The power supply initially outputs 60 mA constant current 2 minigels x 30 mA each The voltage output will be lt 180 V but may rise during the electrophoresis run owing to gradual deple tion
30. ymbol kennzeichnet elektrische und elektronische Ger te die nicht mit dem gew hnlichen unsortierten Hausm ll entsorgt werden d rfen sondern separat behandelt werden miissen Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des Herstellers auf um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come rifiuti municipali indifferenziati e devono invece essere raccolti separatamente Per informazioni relative alle modalita di smantellamento delle apparecchiature fuori uso contattare un rappresentante autorizzato del fabbricante Este simbolo indica que el equipo el ctrico y electr nico no debe tirarse con los desechos dom sticos y debe tratarse por separado Contacte con el representante local del fabricante para obtener mas informaci n sobre la forma de desechar el equipo Denna symbol anger att elektriska och elektroniska utrustningar inte far avyttras som osorterat hushallsavfall och maste samlas in separat Var god kontakta en auktoriserad tillverkarrepresentant f r information angaende avyttring av utrustningen e pvii Please read this entire manual to fully understand the safe and effective use of this product If you have any comments on this manual please send them to us at Hoefer Inc 84 October Hill Road Holliston MA 01746 support hoeferinc com Hoefer
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