EZSeries User Manual
Contents
1. Leica Microsystems Switzerland Ltd Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Specifications Due to the technology of the camera the speci fications are changing regularly Please visit our website www eica microsystems com education for the latest brochure downloads to find the up to date specifications Leica E Series User Manual Specifications Specifications Operating environment Feature Specification Temperature Operating Storage 10 C to 40 C 50 F 104 F 20 C to 55 C 4 F 131 F Relative humidity maximum Operating Storage 10 to 90 noncondensing Altitude maximum Operating Nonoperating 0 m to 2 000 m 0 ft to 6 561 ft 0 m to 10 668 m 0 ft to 35 000 ft Pollution Degree Rating Weight 4 6 kg Ports and connectors Feature Specification EZ4 E or EZ4 W USB one standard USB 2 0 Typ B EZ4 E or EZ4 W Video one standard HDMI connector Typ A EZ4 W Network adapter one RJ 45 connector EZ4 E or EZ4 W Memory card reader supports up to 32 GB Integrated power supply 100 V 240 V 50 60 Hz Automatic adjustment to local voltage Power consumption max 30 W EZ4 E or EZ4 W 15 W ES2 EZ4 Leica E Series User Manual Specifications Dimensions Leica E Series User Manual Dimensions Leica ES2 de 2 So Ss 37 Dime2. Leica E Series User Manual The Camera Remote Con SCAN SSID If OK is selected you will see a list of WiFi access points nearby Pairing Cameras With Remotes Pairing The Leica EZ4 E or Leica EZ4 W and the Li remote control can be paired and then only respond to each other This can be helpful when using multiple cameras and remote cont rols 1 Press the button to start or end the process lil In order to achieve successful pairing and avoid pairing by mistake the second step must be performed within 4 seconds il Ifa timeout is displayed after 4 seconds press the Pair button again to start the process 2 Press and hold the button on your remote you want to define as the pairing button until a corresponding confirmation is displayed on the HD monitor All of the buttons except the 4 button can be used for this Leica E Series User Manual 3 The microscope only responds to the remote control commands from this remote control Resetting to factory setting 1 Press the button 4 to start the process 2 Press the button until a corresponding confirmation is displayed on the HD monitor The Camera Remote Control Optional Care Transport Contact Persons Care Transport Contact Persons General Instructions e Keeping all optical components clean is important for maintaining good optical performance e Always protect the microscope and camera with the plastic cover wh
3. ETES gt RESOLUTION SETUP WIFI only for EZ4 W il The RESOLUTION menu enables you to define the resolution for the live image still image capture and video capture individually This provides optimum results in every situation LIVE resolution of the live image Defines the resolution of the live image CAPTURED resolution of the captured image Defines the resolution of the captured image on the SD card MOVIE resolution of videos Indicates the resolution of the captured videos on the SD card Leica E Series User Manual The Camera Remote Control Optional SETUP CAMERA Camera Settings Main menu Sub menu setup camera EXPOSURE PRESSOK O me gt SETUP CAMERA SETUP ETHERNET NONE o SETUP WIFI only for EZ4 W a The camera settings enable you to control internal parameters such as the date or time reset the camera etc RESET CAMERA This command resets the camera to the factory settings All user informa tion such as white balance resolution etc is reset SET DATE TIME This command sets the date and time as well as the format for display ing them The following formats are available for selection YYMMDD DDMMYY MMDDYY Leica E Series User Manual FLIP IMAGE Select to flip image 180 AUDIO BEEP You can decide to have a tone from the camera for some functions LANGUAGE Select the language of the On Screen Display SELECTING IMAGE OVERLAYS
4. Leica ES2 Leica EZ4 Leica EZ4 E Leica EZ4W User Manual MICROSYSTEMS Congratulations You have just purchased a Leica E Series stereo microscope an excellent choice Viewing entire objects under magnification imparts perspectives and discoveries that would not be possible with the naked eye In developing our stereomicroscopes we have placed great emphasis on simple self explan atory operation However please take the time to read the instruction manual and the notes on operating safety to learn about all the features and capabilities of your instrument so that you can use it safely and to its best advantage Should you have any questions please consult your local Leica representative We are gladly at your service Leica E Series User Manual 2 afety and Use eica EZ4 Leica EZ4 E or Leica EZ4 W yepieces only for Leica EZ4 Photography Using the Leica EZ4 E or Leica EZ4 W et Set a Fa A po T D lt MD lt D ui A da w N ui NI w LS a he Camera Remote Control Optional Care Transport Contact Persons 68 Specifications 70 Dimensions 72 Leica E Series User Manual Contents Safety and Use Adjusting the Resistance of the Focus Drive 32 Symbols Used 7 Safety Instructions 8 Regulatory Compliance Information 10 Changing Eyepieces Only for Leica EZ4 Open Version 34 Instructions for Use 1
5. 2 this device must accept any interference including interference that may cause unde sired operation of the device Canada IC Le pr sent appareil est conforme aux CNR d Industrie Canada applicables aux appareils radio exempts de licence L exploitation est autoris e aux deux conditions suivantes 1 l appareil ne doit pas produire de brouillage et 2 l utilisateur de l appareil doit accepter tout brouillage radio lectrique subi m me si le brouillage est susceptible d en compromettre le fonctionnement Leica E Series User Manual Australia and New Zealand Notices This device equipment complies with the Australian and New Zealand regulatory approv als requirements Safety and Use Instructions for Use Intended use The E Series microscopes are optical instru ments for improving the visibility of objects through magnification and illumination It is used for observing and documenting Place of use Use the instrument in enclosed dust free rooms at 10 C to 40 C only Protect it from oil chemicals and extreme humidity It must be at least 10 cm from the wall and away from flammable substances Leica E Series User Manual Interference Avoid large temperature fluctuations direct sunlight and vibrations These conditions can distort measurements and micrographic images In warm and warm damp climatic zones Li the individual components require special care in order to prevent the build up of
6. Data transmis sion equipment operating in the 2 4 GHz ISM band and using wide band modulation techniques Harmonized EN covering essen tial requirements under article 3 2 of the R amp TTE directive Regulatory Compliance Information FCC Requirements for Operation in the United States FCC radio frequency interference warnings and instructions This equipment has been tested and found to comply with the limits for a Class B digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reason able protection against harmful interference in a residential installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accor dance with the instructions may cause harm ful interference to radio communications However there is no guarantee that interfer ence will not occur in a particular installation If this equipment does cause harmful interfer ence to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the follow ing measures e Reorient or relocate the receiving antenna e Increase the separation between the equip ment and receiver Leica E Series User Manual e Connect the equipment into an outlet on a circuit different from that to which the receiver is connected e Consult the dealer or an experienced radio TV techn
7. SELECT OVERLAY Press to select another overlay Press to activate the selected overlay and leave the menu A If you want to quickly display other overlays press when the live image is displayed Q a to show the next or previous respective overlay CONFIGURING IMAGE OVERLAYS CONFIG OVERLAY Depending on the setting this does not show an overlay only shows the overlay in the live image or shows it in both the live image and the image capture The Camera Remote Control Optional SETUP ETHERNET IP ADDRESS Main menu Sub menu Ethernet You can decide if you want the IP address to be set by your network Dynamic or by you using the onscreen keyboard Static COLOR IP ADDRESS DHCP Dynamic EXPOSURE RESOLUTION If you selected STATIC for the IP address you can input the following using the onscreen keyboard SETUP CAMERA SETUP ETHERNET SETUP WIFI e IP address e IP subnet mask e Gateway IP address il This menu allows you to customize parameters relevant to working in Ethernet mode Consult with your local IT expert for information relating to your local network Main menu Sub menu Ethernet COLOR IP ADDRESS M STATIC EXPOSURE IP ADDRESS STATIC 192 168 1 200 RESOLUTION IP SUBNET MASK 255 255 255 0 SETUP CAMERA GATEWAY IP ADDRESS 192 168 1 1 SETUP ETHERNET SETUP WIFI SETUP WIFI only for EZ4 W pa Leica E Series Us
8. The image is now saved on the SD card Images which are on the SD card can be viewed using the optional remote control See Camera Remote Control on page 55 Get Set 6 While in SD mode you can connect the provided HDMI cable from the camera to an HD monitor or HD projector to share the image Adjusting Settings While in SD Mode Setting a new white balance Return to factory settings Press and hold the service button for 5 seconds Make sure that there is no sample in the Wait for the beep The camera will now reset to beam path and that a standard illumina factory settings tion is available when you reset the camera Press and hold the capture button for 5 seconds Wait for the beep A new white balance is set Leica E Series User Manual Get Set WiFi Mode Capturing and Adjusting Images Using a Wireless Device only available for Leica EZ4 W 1 Switch on the power to the Leica EZ4 W microscope The status light will blink red Wait until the status light is green and one of the mode button s is also green This may take approximately 30 seconds Leica E Series User Manual 3 If a mode button other than WiFi mode is lit green then press the WiFi button and wait until both the status LED and the WiFi mode button is green Follow the directions of your WiFi view ing device to connect to the inter nal WiFi network of the camera WiFi Password leicamicro Open the appropriate app
9. fungus Use in clean rooms Leica E Series microscopes can be used in clean rooms without any problems Safety and Use Handling electrical components Never install any other plug NEMA 5 15P or unscrew any mechanical components unless expressly instructed to do so in the instructions The microscope must be connected to a grounded outlet Always position the microscope so that A you can disconnect it from the power supply at any time The power cable is provided as the power disconnect device Instructions for Use Continued Intervention from the user The microscopes of the E Series contain Li no components that can be main tained or repaired by the user Exceptions are mentioned specifically in this manual Unless otherwise specified in this manual repair and service work may be carried out by authorized Leica technicians only Unauthorized alterations to the instru ment or noncompliant use shall void all rights to any warranty claims Leica E Series User Manual Maintenance Leica E Series microscopes are basically maintenance free To ensure that it always operates safely and reliably we recom mend that you take the precaution of contact ing the responsible service organization Here you can arrange for periodic inspections and conclude a maintenance contract recom mended Accessories Only those accessories may be used that are listed in this user manual or for which safe use has been confi
10. on your mobile device or appropriate software on your wireless enabled PC for viewing capturing and adjusting the camera image Get Set Ethernet Mode Capturing and Adjusting Images Using a Network Connect one end of an ethernet cable not provided to the camera and the other end of the cable to the ethernet connection in your facility 2 Switch on the power to the Leica EZ4 E or EZ4 W microscope The status light will blink red Wait until the status light is green and one of the mode button s is also green This may take approximately 30 seconds If a mode button other than ethernet mode is lit green then press the ethernet button and wait until both the status LED and the ethernet LED mode button is green 5 Follow the directions of your network compatible device to connect to your facili ties network and select the camera Open the appropriate app on your mobile device or appropriate software on your wireless enabled PC for viewing capturing and adjusting the camera image Leica E Series User Manual Get Set The Camera Remote Control Optional The remote control is designed to work A only when the camera is in SD mode and when the camera is used in combination with an HD display The only function which works in any mode is the Info function which shows details about the camera Leica E Series User Manual The Camera Remote Control Optional Optional Remote Control The
11. the illustration 2 Store the instrument so that it is protected against dust The Correct Interpupillary Distance The ideal image The interpupillary distance is correctly Li set if you see a single circular image field when looking at a specimen You may need a short time to become familiar with using the instrument Not to worry after a little while it will become automatic Leica E Series User Manual Note for eyeglass wearers l Ifyou wear glasses fold the eyecups back otherwise fold the eyecups forwards Leica ES2 Setting the eye distance 1 Look into the eyepieces 2 Hold the eyepieces with both hands Push the eyepieces together or separate them until you see a circular image Using the LED Illuminator Illuminating the specimen 1 Position a specimen in the middle of the baseplate 2 Switch the two LED illuminators on or off as desired A After switching off the LEDs remain lit for about another 4 seconds Leica E Series User Manual Tips on use Use incident light for plastic opaque objects Use transmitted light for transmitted light specimens or transparent objects Use the combination of both LED illumina tors for partially transparent objects Leica ES2 Focusing Focusing raises and lowers the stereo Focusing microscope using the focusing drive The 1 Set the magnification changer to the lowest specimen is brought into sharp focus as soon as l
12. 2 Eyepieces and Eyecups Only for Leica EZ4 Open Version 35 Dioptric Correction Only for Leica EZ4 Open Version 36 Graticules Only for Leica EZ4 Open Version 38 Overview Leica ES2 15 Graticules Preparation 39 Transport Placement and Storage 16 Inserting the Graticules 40 The Correct Interpupillary Distance 17 Using the LED Illuminator 18 Focusing 19 Introduction 42 Magnification Display 20 The Basics 43 Adjusting the Resistance of the Focus Drive 21 Leica EZ4 E or Leica EZ4 W Overview 44 Unpacking 46 Overview Leica EZ4 23 ZE Instrument Variant Leica EZ4 E or Leica EZ4 W 24 USB Mode Capturing and Adjusting Images Using a Computer 48 Transport Placement and Storage 25 SD Mode Capturing Images Without a Computer or The Correct Interpupillary Distance 26 Without a Wireless Device 50 Switching on the LED Illuminator 27 Adjusting Settings While in SD Mode 52 Controlling the Automatic Shutoff 28 WiFi Mode Capturing and Adjusting Images Using a Wireless Device Illumination Types 29 only available for Leica EZ4 W 53 Focusing 30 Ethernet Mode Capturing and Adjusting Images Using a Network 54 Magnification Display 31 Leica E Series User Manual 4 Contents Continued he Camera Remote Control Optional Optional Remote Control 56 Viewing Images and Movies With the Optional Remote Control 57 Calling up the Camera Menu 58 COLOR Automatic White Balance 59 COLOR Manual White Balance 60 EXPO
13. 6 A or 10 A fuse Only connect the instrument to a grounded power socket that has been installed according to regulations The power supply voltage has to correspond to the voltage speci fied on the identification label Make sure that the ground connection is neither defective nor interrupted since otherwise a malfunction poses a risk of fatal injury Never unplug the power plug with moist or wet hands There is danger of an electric shock Safety Instructions Continued Instrument safety and EMC Our device has been designed produced and tested in compliance with e IEC EN 61010 1 Electrical equipment for measurement control and laboratory use Safety Part one general requirements e EN60950 1 Information technology equip ment Safety Part one general require ments e Radio interference suppression in compli ance with EN 55011 class B e Noise immunity DIN EN 61326 1 in compliance with Leica E Series User Manual The device meets the requirements of EU directives e 2006 95 EG e 2014 30 EU e 2011 65 EU Low voltage directive EMC directive RoHS directive and carries the CE mark directive The device must be disposed of in accordance with 2012 19 EU WEEE Intended for indoor use only in all EU member states EFTA states and Switzerland Safety and Use Leica EZ4W EN 300328 Electromagnetic compatibility and Radio spectrum Matters ERM Wide band transmission systems
14. Leica EZ4 Graticules Preparation Care instructions The graticule must always be clean and dust free Never touch the graticule with bare hands Always hold the graticule by the edges Leica E Series User Manual Variant 1 10x and 20x eyepieces The 10x and 20x adjustable eyepieces include an insert at the bottom with a snap mount 1 Gently press the two small tabs on the snap mount together and pull out the insert Eyepieces Only for Leica EZ4 Variant 2 16x eyepieces On the 16x adjustable eyepieces the insert is fixed to the eyepiece by a screwed connection 1 Screw the insert out counterclockwise Inserting the Graticules Inserting the graticules is the same for all 4 Put the insert back into the eyepiece 6 Align the graticules by turning the eyepiece eyepiece types Depending on the variant you have to in the tube either press or twist the insert into place 1 Place the insert with the knurled side on the work surface 2 Grasp the graticule so that you see the scale in laterally correct form 3 Carefully take hold of the edge of the grati cule and push it sideways into the holder in the insert Make sure that both plastic holders snap into place 5 Leica E Series User Manual Eyepieces Only for Leica EZ4 Photography Using the Leica EZ4 E or Leica EZ4 W Introduction Thank you for purchasing the Leica EZ4 E or Leica EZ4 W by Leica Microsystems The special design of the Lei
15. SURE 61 RESOLUTION 62 SETUP CAMERA Camera Settings 63 SETUP ETHERNET 64 SETUP WiFi 65 Pairing Cameras With Remotes 67 Care Transport Contact Persons 69 Specifications 71 Leica ES2 73 Leica EZ4 E or Leica EZ4 W 75 Leica EZ4 10x Eyepieces 77 Leica EZ4 without Eyepieces 79 Leica E Series User Manual 5 Safety and Use Symbols Used D Warning of a danger This symbol indicates especially impor A tant information that must be read and complied with Failure to comply can cause the following Read and understand user manual before using this device e Hazards to personnel e Functional disturbances or instruments damaged Warning of hazardous electrical voltage This symbol indicates especially impor tant information that if not observed can cause the following e Hazards to personnel e Functional disturbances or instruments damaged Leica E Series User Manual Danger due to hot surface A This symbol warns against touching hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Safety and Use Safety Instructions A Before installing operating or using the instrument it is mandatory to read this user manual In particular please observe all safety instructions The Safety Concept brochure contains addi tional safety information regarding service work requi
16. aching the power cable 1 Switch on the power to the EZ4 E or EZ4 W microscope The status light will blink red Wait until the status light is green and one of the mode button s is also green This may take approximately 30 seconds Get Set 4 If a mode button other than SD mode is lit green then press the SD mode button and wait until both the status LED and the SD mode button is green Insert the SD memory card into the slot on the side of the Leica EZ4 E or EZ4W until the card clicks into place SD Mode Capturing Images Without a Computer or Without a Wireless Device Continued The capture button LED will turn green The Leica EZ4 E or Leica EZ4 W is now ready to capture images onto the SD memory card Capturing an image Focus the microscope at high magnification then change to the magnification at which you wish to capture the image Do not refocus the microscope You can also use the HDMI output and an HD display not provided as a focusing aid 5 To capture an image onto the SD memory card lightly press the capture button located on the side of the Leica EZ4 E or EZ4 W camera Leica E Series User Manual e You will hear a beep to acknowledge the button was pressed e The status LED flashes red while the image is being captured e You will hear another beep when the Leica EZ4 E or Leica EZ4 W has completed captur ing the image and the status LED will return to steady green
17. ca EZ4 E or EZ4 W makes it a universal highly versatile tool for viewing microscopic specimens and capturing still images or video Leica E Series User Manual Photography Using the Leica EZ4 E or Leica EZ4 W The Basics Your Leica HD camera has many possibili i ties for various applications and condi tions of use Below are some basic concepts to help you get the most performance for your application Leica E Series User Manual Modes of use 1 SD mode e capture onto SD card e use remote control 2 WiFi mode only available for EZ4 W e Wireless connection to wireless device tablet phone PC 3 USB mode e Cabled connection to PC 4 Ethernet mode e Cabled connection to external network 5 Capture button e Captures image onto the SD card Photography Using the Leica EZ4 E or Leica EZ4 W il Note direct connection to an HD display is possible in all modes Aspect ratio is 16 9 except in USB mode where the aspect ratio is 4 3 Leica EZ4 E or Leica EZ4 W Overview 1 LED status indicator e Blinking red means starting up e Green means ready 2 Capture button for SD card capture 3 Mode selection panel 4 SD card slot Leica E Series User Manual Photography Using the Leica EZ4 E or Leica EZ4 W Leica EZ4 E or Leica EZ4 W Overview Continued 1 HDMI for connecting the provided HDMI cable 2 Ethernet socket for connecting an ethernet cable cable not pr
18. ct the lowest magnification again but do not look into the eyepieces Carry out steps 6 to 8 for each objective individually Rotate the eyepiece counterclockwise in the direction as far as it will go 5 diopter settings Dioptric Correction Continued 7 Look into the eyepiece 8 Slowly rotate the eyepiece individually in the direction until the eye sees the object sharply imaged 9 Select the highest magnification and refocus if necessary Now if you adjust the magnification from the highest to the lowest level the specimen is always brought into sharp focus If not repeat the process Leica E Series User Manual Eyepieces Only for Leica EZ4 Graticules Only for Leica EZ4 Open Version Graticules Available eyepiece inserts The transparent graticules can be A Graticules 10 mm 0 1 mm inserted in the adjustable eyepieces of the Leica EZ4 They enable easy measurements at the specimen 5 mm 0 05 mm 100 Div 0 002 100 Div 0 001 150 Div 0 0005 5 mm 0 1 mm i For equipment with a graticule in one eyepiece two adjustable eyepieces are needed Since one eye is focussed on the Stage micrometers 50 mm 0 1 0 01 mm graduation eyepiece with the graticule it needs a second 1 0 001 graduation adjustable eyepiece so that it can be aligned Crosshairs Crosshairs optically with the first Leica E Series User Manual Eyepieces Only for
19. e for eyeglass wearers l Ifyou wear glasses fold the eyecups back otherwise fold the eyecups forwards Leica EZ4 Leica EZ4 E or Leica EZ4 W Setting the eye distance 1 Lookinto the eyepieces 2 Hold the eyepieces with both hands Push the eyepieces together or separate them until you see a circular image Switching on the LED Illuminator Automatic shutoff If the illumination is not operated for 60 minutes it switches off automatically The illumination can be reactivated by pressing a button on the membrane keypad LED illumination 1 Position a specimen in the middle of the baseplate Leica E Series User Manual Switch the two LED illuminators on or off as For a detailed description of the illumina desired Li tor refer to page 29 Use incident light for plastic opaque objects Use transmitted light for transmitted light specimens or transparent objects Use the combination of both LED illumina tors for partially transparent objects Leica EZ4 Leica EZ4 E or Leica EZ4 W Controlling the Automatic Shutoff Automatic shutoff The factory settings of the EZ4 models Li switch the instrument off automatically after one hour without user interaction This automatic shutoff helps to conserve the light emitting diodes and save energy Leica E Series User Manual Disabling the automatic shutoff To disable the automatic shutoff follow these steps 1 Switch the microscope of
20. en the instrument is not in use Included in the delivery pack age of the microscope Be sure power to the camera is off when the dust cover is in place e If any optical surface becomes badly coated with dust or dirt it should be cleaned by blowing the surface with a syringe or brush ing it off with a camel hair brush before attempting to wipe the surface clean e Optical surfaces should be cleaned using a lint free cloth lens cloth or cotton swab moistened with a commercially available glass cleaner Do not use alcohol Leica E Series User Manual Avoid excessive use of solvents The lint free cloth lens cloth or cotton swab should be soaked with solvent but not so wet that solvent runs over the lens Protect the microscope from moisture fumes and acids and from alkaline caustic and corrosive materials Do not store chemicals in the vicinity Protect the microscope from oil and grease Never grease or oil mechanical parts or sliding surfaces It is advisable to enter a service agreement with Leica Service Care Transport Contact Persons Transport Always use both hands to carry the microscope There is a handle on the back of the microscope and an undercut in the front for this purpose Contact address If your instrument no longer works perfectly contact your Leica representative You can find information on worldwide Leica representatives on the Leica website www leica microsystems com
21. er Manual The Camera Remote Co SETUP WiFi only for Leica EZ4 W Main menu Sub menu WiFi CTT INTERAC STANDALONE RESOLUTION SECURITY OPTIONS NONE i SETUP CAMERA PASSWORD 8 CHR TE SETUP ETHERNET CHANNEL AUTO gt SETUP WIFI ENABLE SSID BROADCAST fa This menu allows you to customize parameters relevant to working in WiFi mode INTERFACE STAND ALONE The camera is the access point INTERFACE NETWORK Your network provides the access point and the camera connects to your network by WiFi NAME SSID This is the camera name which will appear You can change the name using the onscreen keyboard Leica E Series User Manual SECURITY OPTIONS NONE This means there is no password SECURITY OPTIONS WPA2 PSK AES This allows you to create a password PASSWORD Create a password using the onscreen keyboard CHANNEL When stand alone is selected as the interface this allows the WiFi channel to be set automatically or by the user ENABLE SSID BROADCAST When stand alone is selected as the interface you can decide if you want the camera name to be seen as available to connect WIFI PASSWORD leicamicro The Camera Remote Control Optional SETUP WiFi Continued Main menu COLOR EXPOSURE RESOLUTION SETUP CAMERA SETUP ETHERNET SETUP WIFI Sub menu WiFi INTERFACE NAME SSID SECURITY OPTIONS PASSWORD 8 CHR SCAN SSID M NETWORK ICC50 W_2345 NONE OK
22. evel 10 it is in the focal point of the objective The focusing drive can be operated either left or right handed 2 Set the working distance to approx 100 mm for coarse focusing Focusing drive torque Leica E Series User Manual Leica ES2 3 Set the magnification changer to the second level 30 4 Use the focusing drive once again for fine focus After you have adjusted the image Li sharpness at high magnification it is maintained even if you switch to a lower magnification parfocal Magnification Display You can read the configured magnifica a tion on the right hand rotary knob of the Leica ES2 The additional magnification provided by the eyepieces is already incorpo rated in the scale Leica E Series User Manual Leica ES2 Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too easy or too difficult to adjust No problem the resistance can be adjusted individually depending on your personal preferences 1 Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing Leica E Series User Manual Leica ES2 Leica EZ4 Leica EZ4 E or Leica EZ4 W Overview Leica EZ4 1 10x fixed eyepieces for eyeglass wearers 2 Carrying handle 3 Magnification changer 4 Focusing drive 5 Integrated LED incident light illumination 6 Illumination control 7 In
23. f 2 Press and hold the and buttons on the light control unit simultaneously while you switch on the microscope The LEDs flash twice slowly and then remain switched on The automatic shutoff is now disabled Leica EZ4 Leica EZ4 E or Leica EZ4 W Enabling the automatic shutoff To re enable the automatic shutoff follow these steps 1 Switch the microscope off 2 Press and hold the G and buttons on the light control unit simultaneously while you switch on the microscope The LEDs flash three times slowly and then remain switched on The automatic shutoff is now re enabled Illumination Types Three level incident light illumination Regulating brightness The incident light illumination is made The brightness is adjusted using the and i up of 5 LEDs Two three or five diodes are keys and affects all switched on LEDs switched on depending on the level Accord ingly the angle of incidence of the light beam on the object is switched from high to low DOD D Press 1x Press 2x Press 3x Press 4x Press 1x Five diodes greatest Top three diodes shadow Two diodes side light Incident light off Switch on transmitted light brightness for incident less uniform lighting for emphasizes structures and for transparent objects light objects objects with highly uneven increases the contrast and transmitted light surfaces specimens Leica E Series User Manual Le
24. ica EZ4 Leica EZ4 E or Leica EZ4 W Focusing Focusing raises and lowers the stereo Focusing microscope using the focusing drive The 1 Set the magnification changer to the lowest specimen is brought into sharp focus as soon as level 8 it is in the focal point of the objective The focusing drive can be operated either left or right handed 2 Set the working distance to approx 100 mm for coarse focusing Focusing drive torque Leica E Series User Manual Leica EZ4 Leica EZ4 E or Leica EZ4 W 3 Set the magnification changer to the second level 35 4 Use the focusing drive once again for fine focus After you have adjusted the image sharp Li ness at high magnification it remains constant even if you switch magnification levels parfocal Magnification Display You can read the configured magnifica i tion on the right hand rotary knob of the Leica EZ4 models The additional magnification provided by the eyepieces is already incorpo rated in the scale Leica E Series User Manual Leica EZ4 models with open tubes On the Leica EZ4 model without i eyepieces only the magnification factors of the magnification changer are indicated 0 8 1 1 25 1 6 2 2 5 3 3 5 Different total magnifications are possi ble depending on which eyepieces are used with the Leica EZ4 The magnification of the eyepieces is indicated on their outer surfaces e g 10x 20 16x 16 or 20x 12 Leica EZ4 Le
25. ica EZ4 E or Leica EZ4 W Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too easy or too difficult to adjust No problem the resistance can be adjusted individually depending on your personal preferences 1 Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focus ing Leica E Series User Manual Leica EZ4 Leica EZ4 E or Leica EZ4 W Eyepieces Only for Leica EZ4 Changing Eyepieces Only for Leica EZ4 Open Version Changing eyepieces Various eyepieces are available for the Leica EZ4 allowing the stereomicroscope to be adapted even better to the specific require ments The following eyepieces are available for selec tion e Eyepieces replaceable fixed or adjustable 10x 20 16x 16 or 20x 12 e Eyepieces for eyeglass wearers 10x 20B and 16x 15B fixed and adjustable The interchangeable eyepieces also offer the following advantages e The dioptric range is adjustable from 5 to 5 e Measurement graticules can be used see page 38 and the following Leica E Series User Manual Adjustable eyepieces for eyeglasses with straight eyecups Eyepieces Only for Leica EZ4 Eyepieces and Eyecups Only for Leica EZ4 Open Version Eyecups The eyecups can be replaced Risk of infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infection
26. ician for help FCC information to user This product does not contain any user service able components and is to be used with approved antennas only Any product changes or modifications will invalidate all applicable regulatory certifica tions and approvals FCC guidelines for human exposure This equipment complies with FCC radiation exposure limits set forth for an uncontrolled environment This equipment should be installed and operated with minimum distance of 20 cm between the radiator and your body This transmitter must not be co located or oper ating in conjunction with any other antenna or transmitter Safety and Use FCC caution e Any changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate this equipment e This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation e This device and its antenna s must not be co located or operation in conjunction with any other antenna or transmitter Regulatory Compliance Information Continued Canada IC This device complies with Industry Canada licence exempt RSS standard s Operation is subject to the following two conditions 1 this device may not cause interference and
27. mote control to show the camera menus on the monitor 3 Press the A w and gt buttons to select a menu item 4 Press the button to confirm a menu item 5 Press the camera menus button again to hide the Leica E Series User Manual The Camera Remote Control Optional COLOR Automatic White Balance Enabling automatic white balance Main menu Sub menu color 1 Press the E button on the remote control Baw 2 Call up the COLOR entry EXPOSURE WB MODE RESOLUTION ELSA 3 Set the value for WB MODE to AUTO SETUP CAMERA BLUE SETUP ETHERNET BLACK LEVEL SETUP WIFI HUE SATURATION 4 Press the El button to exit the menu SETUP WIFI only for EZ4 W The values for RED and BLUE cannot be adjusted if the WB MODE setting is set to AUTO The COLOR function makes it possible to adapt the camera chip to the ambient light so that color neutral images can be acquired Leica E Series User Manual The Camera Remote Control Optional COLOR Manual White Balance Main menu Sub menu color COLOR SET WB PRESS OK EXPOSURE WB MODE EM MANUAL RESOLUTION RED LEVEL SETUP CAMERA BLUE LEVEL SETUP ETHERNET BLACK LEVEL SETUP WIFI HUE SATURATION SETUP WIFI only for EZ4 W The COLOR function makes it possible to adapt the camera chip to the ambient light so that color neutral images can be acquired Leica E Series User Manual The Came
28. nsions Leica E Series User Manual Leica ES2 Continued IPD 50 to 75 W Leica EZ4 E or Leica EZ4 W 372 75 Max 279 100 75 Max Leica E Series User Manual Dimensions Leica EZ4 E or Leica EZ4 W Continued IPD 50 to 75 198 Leica EZ4 10x Eyepieces n da n t E Leica E Series User Manual Leica EZ4 10x Eyepieces Continued IPD 50 to 75 Leica EZ4 without Eyepieces Leica E Series User Manual Dimensions Leica EZ4 without Eyepieces Continued The statement by Ernst Leitz in 1907 With the User For the User describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems We have developed five brand values to live up to this tradition Pioneering High end Quality Team Spirit Dedication to Science and Continuous Improvement For us living up to these values means Living up to Life INDUSTRY DIVISION The Leica Microsystems Industry Division s focus is to support customers pursuit of the highest quality end result Leica Microsystems provide the best and most innovative imaging systems to see measure and analyze the microstructures in routine and research industrial applications materials science quality control forensic science investigation and educational applications www leica microsystems com Leica Microsystems an international c
29. ompany with a strong network of worldwide customer services Active worldwide Tel Fax Australia North Ryde 61 288703500 2 9878 1055 Austria Vienna 43 148680500 1486805030 Belgium Diegem 32 27909850 2790 98 68 Brazil S o Paulo 55 11 2764 2411 11 2764 2400 Canada Concord Ontario 1 8002480123 847 405 0164 Denmark Ballerup 45 44540101 44540111 France Nanterre Cedex 33 811000664 156052323 Germany Wetzlar 49 6441294000 6441294155 India Mumbai 91 226 1880 200 226 1880 333 Italy Milan 39 02 574 861 02 574 03392 Japan Tokyo 81 3 5421 2800 3 5421 2896 Korea Seoul 82 2514 65 43 251465 48 Netherlands Rijswijk 31 70 4132 100 70 4132 109 People s Rep of China Hong Kong 852 25646699 2564 4163 Shanghai 86 21 6039 6000 21 6387 6698 Portugal Lisbon 351 21 388 9112 21 385 4668 Singapore 65 6550 5999 6564 5955 Spain Barcelona 34 934949530 93 494 95 32 Sweden Kista 46 8625 45 45 86254510 Switzerland Heerbrugg 41 717263434 717263444 United Kingdom Milton Keynes 44 8002982344 1908 577640 USA Buffalo Grove Illinois 1 8002480123 847 405 0164 eua MICROSYSTEMS
30. ovided 3 USB for connecting the provided USB cable Front Infrared window Receives the signal from the optional remote control Leica E Series User Manual Photography Using the Leica EZ4 E or Leica EZ4 W Unpacking Please do not discard any of the packing materials They should be used for safely storing and transporting the instrument should the need arise The contents include CD with imaging software If you ordered the handheld remote you received HDMI cable and USB cable the remote with batteries Leica E Series User Manual Photography Using the Leica EZ4 E or Leica EZ4 W Get Set Leica E Series User Manual Get Set USB Mode Capturing and Adjusting Images Using a Computer 10x eyepieces have a fixed circular field Li of view of 18 mm or more diameter The Leica EZ4 E or Leica EZ4 W s field of view however is rectangular with a diagonal of 16 4 mm to ensure an even field on the digital image As a result the image field of the camera is smaller than the object field seen through the eyepieces Installing and connecting 1 Insert the provided CD into your computer and follow the instructions to load the soft ware Leica E Series User Manual 2 Insert the provided USB cable into the camera s USB port and attach the other end of the cable to a USB 2 0 port of your computer The status light will blink red Wait until the status light is green and one of the mode but
31. ra Remote C Adjusting the white balance manually recommended Move the specimen out of the field of view so that only the illumina tion is seen 1 Press the E button on the remote control Call up the COLOR entry Set the WB MODE to MANUAL If necessary correct the values for RED and BLUE until the image shows a uniform gray area Press the E button to exit the menu EXPOSURE Automatic exposure Main menu Sub menu exposure 1 Press the E button on the remote control COLOR EXPOSURE RESOLUTION EXP MODE 2 Call up the command for automatic exposure see diagram at the left 3 Set the value for EXP MODE to AUTO SETUP CAMERA BRIGHTNESS Der tah 4 Correct the values for BRIGHTNESS and GAMMA until you obtain the desired results for the image Manual exposure 1 Press the E button on the remote control Main menu Sub menu exposure 2 Call up the command for manual exposure see diagram at the left COLOR gt BIA EXP MODE MANUAL EXPOSURE 3 Set the value for EXP MODE to MANUAL RESOLUTION GAIN SETUP CAMERA BRIGHTNESS 4 Correct the values for EXPOSURE GAIN and GAMMA until you SETUP ETHERNET GAMMA obtain the desired results for the image SETUP WIFI SETUP WIFI only for EZ4 W Leica E Series User Manual The Camera Remote C RESOLUTION Main menu Sub menu resolution EXPOSURE
32. rements and the handling of the microscope accessories as well as general safety instructions You can combine individual system articles with articles from external suppliers Please read the user manual and the safety requirements of the supplier To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals Leica E Series User Manual Warning of a danger This symbol indicates especially impor A tant information that is mandatory to read and observe Failure to follow these instructions may pose hazards to personnel cause instrument malfunctions or damage the instrument Hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to follow these instructions may pose hazards to personnel cause instrument malfunctions or damage the instrument Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Safety and Use EC Declaration of Conformity Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Decla ration of Conformity Technical safety To connect the Leica E Series microscopes to power use a power cable that meets the power cable specifications in your country The power socket used has to be equipped with a 1
33. remote control can only work in SD mode except for the i button which will display information about the camera on an HD display The remote must be pointed at the IR receiver of the camera without obstructions Leica E Series User Manual The Camera Remote Control Optional Start stop video recording Save still image to SD card Stop continue live image pause play movie clip View files on SD card Move to previous image on SD card Move to next image on SD card Brightness adjustment up or down Pairing button Show hide information box White balance set button Call up the camera menu Arrow keys for navigation OK to confirm Viewing Images and Movies With the Optional Remote Control A If you have purchased the optional remote the Leica HD camera shows images and movie clips directly on an HD moni tor 1 Thumbnail view mm first press e up down left right select thumbnail e Show image in full screen or playback movie clip e next image previous image Playback fullscreen view e CA gt Play movie clip e left right gt fast forward rewind 2 To return to live view press mma Thumbnail view Leica E Series User Manual The Camera Remote Control Optional Calling up the Camera Menu A The remote control can only work when Opening and closing the camera menu the camera is in SD mode 1 Point the remote control towards the camera 2 Press the E key on the re
34. rmed by Leica Microsystems Danger of infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Safety and Use Dangers during use The microscope illumination is in the exempt group risk group 0 according to EN 62471 2008 when used according to its intended use Never look directly into the LED beam A of the illumination equipment either with or without optical instruments as this increases the risk class Failure to observe this notice poses a risk of eye damage Leica ES2 Leica E Series User Manual Leica ES2 Overview Leica ES2 1 10x fixed eyepieces for eyeglass wearers 2 Carrying handle 3 Two level magnification changer 10x and 30x 4 Focusing drive 5 Integrated LED incident light illumination 6 Integrated LED transmitted illumination 7 Switch for incident and transmitted light Leica E Series User Manual Leica ES2 Transport Placement and Storage Built in carrying device Setting up the microscope All Leica E Series microscopes are 1 Place the microscope on a flat tabletop l equipped with a carrying handle for convenient and safe transport 2 Connect the power supply to a grounded socket Leica E Series User Manual Leica ES2 Packing the device away 1 After use wind up the cable as shown in
35. s of the eye The risk can be minimized by the use of individual eyecups Eyeglass eyepiece with adjust Eyepiece for users who do not Fixed eyepiece with inclined able diopter and straight wear eyeglasses with adjustable eyecup eyecups diopter and inclined eyecup lt Leica E Series User Manual Eyepieces Only for Leica EZ4 Dioptric Correction Only for Leica EZ4 Open Version Dioptric correction The adjustable eyepieces for the Leica EZ4 are also available with built in diop tric correction allowing the microscope to be used without glasses even by those with vision problems The correction comprises a maxi mum of 5 diopter settings Leica E Series User Manual Users with normal vision do not need adjust able eyepieces An adjustable eyepiece is suffi cient for mild shortsightedness If vision is extremely poor or when graticules are used two adjustable eyepieces are needed When the diopters are regulated exactly the sharpness is constant over the entire magnifi cation range parfocal Once the diopters have been set the procedure does not have to be repeated Simply restore your diopter values on the eyepiece before beginning work with the stereomicroscope Eyepieces Only for Leica EZ4 Adjusting Te Set the dioptric correction for both eyepieces to 0 Select the lowest magnification and focus on a flat specimen Select the highest magnification and read just the sharpness Sele
36. tegrated LED transmitted illumination Leica E Series User Manual Leica EZ4 Leica EZ4 E or Leica EZ4 W Instrument Variant Leica EZ4 E or Leica EZ4 W 1 10x fixed eyepieces for eyeglass wearers 2 Integrated digital camera with SD card slot 3 Mode selection panel EZ4 E or EZ4 W 4 Carrying handle 5 Magnification changer 6 IRreceiver for optional remote control 7 Focusing drive 8 Integrated LED incident light illumination 9 Illumination control 10 Integrated LED transmitted illumination Leica E Series User Manual Leica EZ4 Leica EZ4 E or Leica EZ4 W Transport Placement and Storage Built in carrying device Setting up the microscope Packing the device away All Leica E Series microscopes are 1 Place the microscope on a flat tabletop 1 After use wind up the cable as shown Li equipped with a holder for convenient in the illustration and safe transport 2 Connect the power supply to a grounded socket 2 Store the instrument so that it is protected against dust Leica E Series User Manual Leica EZ4 Leica EZ4 E or Leica EZ4 W The Correct Interpupillary Distance The ideal image The interpupillary distance is correctly Li set if you see a single circular image field when looking at a specimen You may need a short time to become familiar with using the instrument Not to worry after a little while it will become automatic Leica E Series User Manual Not
37. ton s is also green This may take approximately 30 seconds 3 Switch on the power of the EZ4 E or EZ4 W microscope Get Set USB Mode Capturing and Adjusting Images Using a Computer Continued 5 Ifa mode button other than USB mode is lit Note the image aspect ratio while in USB Note the image resolution on the HD green then press the USB mode button and mode is 4 3 To avoid distortion if your Li projector or monitor is limited to the live wait until both the status LED and the USB projector or monitor is set to a 16 9 aspect ratio image resolution setting in the software mode button is green you will need to adjust the aspect ratio of the projector or monitor to 4 3 using the controls of the projector or monitor 6 You are ready to launch the Leica software Follow the software instructions to adjust and capture an image 7 While in USB mode you can connect the provided HDMI cable from the camera to e an HD monitor or HD projector to share the SS image 4 3 aspect ratio 4 3 aspect ratio image on a screen set to an aspect ratio of 16 9 Leica E Series User Manual Get Set SD Mode Capturing Images Without a Computer or Without a Wireless Device In order to use this mode you must have an SD card not provided with free memory on it If the SD memory card is full the capture button LED will not light green and you will not be able to capture any more images Leica E Series User Manual Att
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