DEN-1 - User manual
Contents
1. and have it checked by a repair and maintenance technician DURING OPERATION Do not operate the unit in environments with aggressive or explosive chemical mixtures Do not operate the unit if it is faulty or has been installed incorrectly Do not use outside laboratory rooms The Select and Install buttons are used only for calibration of the unit according to p 3 4 Do not press the buttons in other cases as this can cause calibration reset and recalibration will be needed BIOLOGICAL SAFETY Itis the user s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment Page 3 2 General Information DEN 1 densitometer has been designed for solution turbidity measurement in the range of 0 3 5 0 McFarland units 100x10 1500x10 cells ml DEN 1 is capable of measuring solution turbidity in a wider range 5 0 15 0 McFarland units however it is necessary to remember that in this case the standard deviation values increase DEN 1 densitometer is used for determining concentration of cells bacterial yeast cells in the fermentation process for detection of susceptibility of microorganisms against antibiotics for identification of microorganisms with various test systems for measuring optical density at fixed wavelength and for quantitative evaluation of soluted substance concentration The operation principle is based on optical density measurement wi2. fig 2 2 0 0 means that the unit is calibrated and ready for operation if no any tube inserted CC flashing means that the unitis not calibrated Calibrate the unit EE means that operator error message Switch OFF the unit and then switch ON again 4 4 Shake the tube with the solution it is recommended to use vortex e g Personal vortex V 1 plus for shaking and insert it into the socket fig 2 1 The McFarland value for the solution will be shown on the display fig 2 2 4 5 Glass and transparent plastic tubes 16 or 18 mm in external diameter can be used for work with DEN 1 densitometer An adapter must be used for work with tubes which are 16 mm in external diameter A Note The unit must be calibrated each time a tube type e g with different outer diameter bottom shape or different material transparent plastic tubes is changed 4 6 After finishing the operation switch OFF the unit using the Power switch position O Disconnect the power supply from electric circuit Fig 2 Front panel Page 7 5 Specifications The unit is designed for operation in cold rooms incubators and closed laboratory rooms at ambient temperature from 4 C to 40 C and maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C 5 1 Light SOURCE Size i si sds ieewesha stints riean ada aaea roaa an araea a e arana LED 9 25 Wavelengths aer ali a ira iso as
3. A 565 15nm 5 3 McFarland unitrang anena sias iii oi iii 0 3 15 0 McF 5 4 Display resOlUtION L Lisas ias s sa asian caves ainiai io i ii i i i 0 1 McF 5 5 Accuracy of the full scale 2 0 2 ceecceeeceeeeeceneeeeeeeeeeeeeeeeteecaeeeeesessnaaeeeeeeneaees 3 5 6 Measurement ime si sreothan airea e aE ESO EA A aE 1 sec 5 72 Sample VOIUM ss iais hace dass eee a aarti eee 2 ml minimum 5 8 Recommended external diameter of tube 18 mm or 16 mm using adapter A 16 59 Display shes seeds ins seks aaa eee sta as vais aa I aa a ta I LED 910 DIMENSIONS draonien tl ii sis iii ia a 165x115x75 mm 5 11 Input current power CONSUMPTION ee aka aka 12V 80 mA 1W 5 12 External power supply unit input AC 100 240 V 50 60 Hz output DC 12 V SIS WEIG gt Lasse sietas tens are i ais teed sc face i i a aa i E esate 0 7 kg Accurate within 10 prionai Description Catalogue number accessories A 16 Adaptor for tubes 16 mm in external diameter BS 050102 AK CKG16 Calibration kit for glass tubes BS 050102 BK 16 mm in diameter CKG18 Calibration kit for glass tubes BS 050102 CK 18 mm in diameter Biosan is committed to a continuous programe of improvement and reserves the right to alter design and specifications of the equipment without additional notice Page 8 6 Maintenance 6 1 If the unit reguires maintenance disconnect the unit from the electric circuit and contact Biosan or your local Biosan representa
4. D Medical Biological Research amp Technologies DEN 1 Densitometer Suspension turbidity detector Me Unity biofan Operating Manual for version Certificate V 1AW Contents 1 Safety Precautions General Information Getting Started Operation Specifications Maintenance Warranty and Claims oOo nN O oa A OO N Declaration of Conformity Page 2 1 Safety Precautions The following symbol means Caution Make sure you have fully read and understood the present Manual before using the equipment Please pay special attention to sections marked by this symbol GENERAL SAFETY Use only as specified in the operating manual provided The unit should be saved from shocks and falling After transportation or storage keep the unit under room temperature for 2 3hrs before connecting it to the electric circuit Use only cleaning and decontamination methods recommended by the manufacturer Do not make modifications in design of the unit ELECTRICAL SAFETY Connect only to a external power supply unit with voltage corresponding to that on the serial number label Use only the external power supply unit provided with this product Ensure that the power switch and external power supply unit are easily accessible during use Disconnect the unit from the external power supply unit before moving If liquid penetrates into the unit disconnect it from the external power supply unit
5. d differently Page 4 3 Getting started 3 1 Unpacking Remove packing materials carefully and retain them for future shipment or storage of the unit Examine the unit carefully for any damage incurred during transit The warranty does not cover in transit damage 3 2 Complete set Package contents Standard set DEN 1Densitometer suspension turbidity detector 1 pce external power supply UNIt oe eee cee eee ceeteeeeeeeeeeeeeeeeeeeceeeeeeteeseaeeeeess 1 pce gt Operating Manual Certificate ciana 1 copy Optional accessories As16 adapter fOr tubes aiian a a on request CKG16 calibration kit for glass tubes 16 mm in diameter on request CKG18 calibration kit for glass tubes 18 mm in diameter on request 3 3 Set up Plug the external power supply unit into the 12 V socket at the rear side of the unit fig 1 2 Place the unit on the horizontal even working surface 12 34 Fig 1 Rear panel 3 4 Calibration The device is pre calibrated at the factory for operation with the glass tubes 18 or 16 mm in external diameter see the label on the bottom side of the unit at temperature range from 15 C to 25 C and saves calibration data when being switched off Note For work with other type tubes e g with different outer diameter bottom shape or different material e g transparent plastic tubes it is necessary to perform calibration in the specified
6. oltage Directive 2006 95 EC RoHS 2011 65 EC WEEE 2002 96 EC amp 2012 19 EU SIA BIOSAN Ratsupites 7 build 2 Riga LV 1067 Latvia EN 61326 1 Electrical equipment for measurement control and laboratory use EMC requirements General requirements EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements We declare that this product conforms to the requirements of the above Directive s Z AP Signature Svetlana Bankovska Aleksandr Shevchik Managing director Engineer of R amp D 42 06 2013 A2 06 2043 Page 11 Biosan SIA Ratsupites 7 build 2 Riga LV 1067 Latvia Phone 371 6742 6137 Fax 371 6742 8101 http www biosan lv Version 1 04 October 2013
7. s many times as many points the chosen calibration curve has If a standard is not available press the Select button for the next calibration value to be displayed After installing the last standard value 5 0 or skipping it the Select button the unit exit the calibration mode automatically The unit is ready for operation Note If pressing the Install button during the calibration process does not cause switching to the next standard value it means that the currently inserted in the densitometer socket Standard has lower turbidity value than the previous standard The reason is that the inserted standard solution turbidity does not correspond to the required value the standard is to be shaken or replaced After finishing the calibration switch OFF the unit using the Power switch position O Disconnect the external power supply unit from electric circuit 4 Operation Recommendation during operation e Keeping the unit switched on for 15 min before starting the operation for the operation mode stabilisation is recommended e lf flat bottomed tubes are used the solution level should be higher than 7 mm from a tube bottom if round bottomed tubes are used the level should be higher than 12 mm from a tube bottom 4 1 Connect the external power supply unit to electric circuit 4 2 Switch ON the unit using the Power switch Fig 1 1 on the rear panel position I 4 3 The following indication may be shown on the display
8. th digital presentation of the results in McFarland units The unit is calibrated at the factory and saves calibration data when being switched off However in can be calibrated by 2 6 points in 0 5 5 0 McFarland unit range if necessary Both commercial standards e g produced by bioMerieux Lachema etc and the standards prepared in the laboratory can be used for calibration Table 1 shows the data provided by the McFarland standard manufacturer bioMerieux Table 1 Interpretation of McFarland Standard results into corresponding numeric va lues of bacterial suspension concentration and their optical density at 550 nm McFarland Composition Interpretation Standard Concentration Bacterial Theoretical optical BaSO concentration density at 550 nm 05 2 40x10 mol l 150x10 cells ml 0 125 4 4 80x10 mol l 300x10 cells ml 0 25 2 9 60x10 mol l 600x10 cells ml 0 50 3 1 44x10 mol l 900x10 cells ml 0 75 4 1 92x10 mol l 1200x10 cells ml 1 00 5 2 40x10 mol l 1500x10 cells ml 1 25 Bacterial concentration depends on microorganism size The numbers represent an average value valid for bacteria For yeasts which are larger in size these numbers should be divided by about 30 Values correspond to optical densities of bacterial suspensions The BaSO solutions optical density values differ because the particle size and form differ from those of bacteria and light is diffracte
9. tive 6 2 All maintenance and repair operations must be performed only by gualified and specially trained personnel 6 3 Standard ethanol 75 or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and decontamination of the unit Page 9 7 Warranty and Claims 7 1 The Manufacturer guarantees the compliance of the unit with the requirements of Specifications provided the Customer follows the operation storage and transportation instructions 7 2 The warranted service life of the unit from the date of its delivery to the Customer is 24 months Contact your local distributor to check availability of extended warranty 7 3 If any manufacturing defects are discovered by the Customer an unsatisfactory equipment claim shall be compiled certified and sent to the local distributor address Please visit www biosan lv Technical support section to obtain the claim form 7 4 The following information will be required in the event that warranty or post warranty service comes necessary Complete the table below and retain for your records Model DEN 1 Densitometer Suspension turbidity detector Serial number Date of sale Page 10 8 Declaration of Conformity Eguipment name Type of eguipment Directive Manufacturer Applied Standards Signature Date Declaration of Conformity DEN 1 Densitometer EMC Directive 2004 108 EC Low V
10. tubes Perform calibration in the following sequence from the lower calibration value to the higher values 0 5 1 0 2 0 3 0 4 0 and 5 0 Use atleast 2 points for calibration Page 5 To perform calibration 3 4 1 3 4 2 3 4 3 3 4 4 3 4 5 3 4 6 3 4 7 3 4 8 Page 6 Switch ON position I the unit with the Power switch Fig 1 1 on the rear panel Press the Select button fig 1 3 on the rear panel use a thin pin of maximum diameter 2 mm for pressing the Select and Install buttons A flashing 0 5 indication will be shown on the display showing that the unit is ready to save calibrations value of the first point 0 5 McF Note Shake the tube with the standard solution if necessary it is recom mended to use vortex e g Personal Vortex V 1 plus for shaking Insert the tube with the standard solution corresponding to the calibration point value into the socket of Densitometer fig 2 1 Press the Install button using a pin fig 1 4 First point 0 5 will be saved in the unit memory and the next calibration value will be displayed flashing 1 0 indication will be displayed Note It is recommended to calibrate as many points as possible to obtain precise results The minimum requirement is to calibrate 2 points closest to the working range limits e g 0 5 and 5 0 for operation in 0 5 5 0 McF unit range Repeat steps 3 4 3 3 4 4 until the calibration is completed 1 5 times i e a
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