Perfusion Valve Controller User`s Manual
Contents
1. rf Figure 1a ne Figure 1b Syri ringe E yringe yringi Stopcock Stopcock Luer Female to Barb Connector Luer Female to Barb Connector Tygon Tubing Tygon Tubing Tube to Tube connector PS C Flex tubing Y Connector Pinch Valve C Flex tubing Normally Open Side ais to Tube connector Pinch Valve Normally Closed Side Normally Open Side Tube to Tube connector Tygon tubing Normally Closed Side Tygon tubing PE 160 Tubin yr g Fits inside Tygon tubing This line flows to waste PE 160 Tubing a Fits inside Tygon tubing Manifold Manifold Publication 5713 001 REV A 2 Perfusion Valve Controller Model VC 8 Warner Instruments Operating Directions conta The VC 8 Valve Controller is initially assembled by first attaching the VALVE BRACKET to the RING STAND as shown in Figure 2 below This is followed by insertion of C Flex tubing into the pinch valves Depending on the flow arrangement you re using cut eight 8 or sixteen 16 pieces of C Flex tubing to length Attach a tube to tube connector to each end if using the system shown in Figure 1a Attach a tube to tube con nector to only the lower end if using the system shown in Figure 1b Mount the tubing to the valves as shown in Figure 1 NOTES 1 A slight stretch of the tubing while mounting will facilitate insertion into the pin2. valve is in the off position Pinch valves are designed to work with silicone C Flex or other similar type tubing All systems are supplied with a small quantity of C Flex tubing Response times for these valves varies with solution viscosity and tubing However typical times are 20 to 50 ms NOTE Minimizing the length of C Flex or silicone tubing used will reduce the gas permeability of the system Features e Choice of 3 valve types e 8 channel system e Spill sensor Manual and computer control Publication 5713 001 REV A s Perfusion Valve Controller Model VC 8 Warner Instruments Operating Directions Setup Before beginning setup take inventory of the plastic fittings supplied with the complete system These fittings include eight 8 each of 1 16 ID tube to tube connectors 1 16 ID tube to tube Y connectors 1 16 ID Luer to female barb connectors and stopcock with Luer connector Connectors are shown in order below Plastic tubing can be attached to these connectors with little effort DD STD TA Valve System Figures 1a and 1b below illustrate two configurations wherein the perfu sion system operates in either a stopped flow or continuous flow to waste mode In general the shortest response time of the system to selection of a solution will be achieved by keeping the tubing length between the MAN IFOLD and sample as short as possible
3. Perfusion Valve Controller Model VC 8 WARNER INSTRUMENTS A Harvard Apparatus Company CE Publication 5713 001 REV A WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any ch
4. all valves Ext Analog In In BNC connection for valve selection Active valve opened by analog voltage Event Marker Logic level pulse 500 ms nominal rear panel BNC connector Manifold 2 1 4 1 or 6 1 nominal dead space Power 110 to 130 or 200 to 250 VAC single phase 50 60 Hz 20 W Operating Temperature 10 to 40 C 50 to 104 F Dimensions H x W x D 89 x 203 x 305 mm 3 5 x 8 0 x 12 in Weight Shipping Weight 3 7 kg 8 lb 4 6 kg 10 Ib Operating Conditions Equipment is intended to be operated in a controlled laboratory environment Temperature 0 to 40 C Altitude Sea level to 2000 m Relative Humidity 0 to 95 Publication 5713 001 REV A Declaration of Conformity CE MARKING LVD amp EMC Application of Council Directive 73 23 EEC 89 336 EEC Standard s to which conformity is declared EN61010 1 1993 EN55022 Class A EN61000 3 2 EN61000 3 3 EN50082 1 1992 EN61000 4 2 EN61000 4 3 ENV50204 EN610000 4 4 EN610000 4 8 EN610000 4 11 Manufacturer s Name Warner Instruments LLC Manufacturer s Address 1125 Dixwell Avenue Hamden CT 06514 T 203 776 0664 Eguipment Description Valve Controller Eguipment Class Class I ITE Class A Model Numbers VC 8 Controller I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Hamden Connecticut U S A Signature Jud MA Full Name Ralph Abate A WARNER INSTRUMENTS A Harvard App
5. anges as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 2 Perfusion Valve Controller Model VC 8 Warner Instruments Table of Contents Introductorio 2 FOIOS aula lalla 2 Operating DIrectionS srsesrrresee riser se see ee see ce seenenee 3 SAD RR RR OR aaa 3 Valve SYSIOM ii a ara Ai 3 Overflow SO aii ia aaa 4 Control Description ind 6 Front Panella aida E 6 PTL ANOQUIS A s lmele 6 Command Input Toggle mimsimaavaanannaanannannannannannenni 6 OVEMIOW od iaa 7 Rear Panels A TAO 7 D Gonnecior s nale 7 A lare ian 7 Standby I ento snob O Ne ion di 7 EXE ANQIOG 2 lalla doi 8 Event Oll sisimas a 8 Power Input Module wemararnnananesanananenaaananennaana 8 AC Conversion asl 10 Instructions for Use qa nia 9 Fow Adjustment cocoa 9 Maintenance 10 CleaniNg AA tenet ree deters AM ATA 10 Wii id ni 11 APPO ii E 12 Specifications a fra aaa 12 Declaration of Conformity aramauaseananeannseanasnaunne 13 Publication 5713 001 REV A Perfusion Valve Controller Model VC 8 Warner Instruments Introduction The VC 8 Valve Control System lies at the heart of a multi valve perfusion sys tem designed to automate and control the delivery of solutions to Warner Instruments imaging and recording chambers However its flexible design allows the system to be used in many applicat
6. aratus Company Position Business Manager
7. ch valve Keep the length of C Flex tubing as short as is feasible 2 The solenoid pinch valves will not transition between open and closed states prior to insertion of the C Flex tubing syringe holder 0 Ty syringe i i Tygon tubing valve bracket C Flex tubing valve controller ring stand Next attach the SYRINGE HOLDER to the RING STAND Affix a stopcock with Luer connector to each syringe and mount into the SYRINGE HOLD ER Now cut six 8 pieces of Tygon tubing just long enough to run from the stopcock to the C Flex tubing Attach a Luer to female barb connector to the top end and a tube to tube connector to the valve end and make attachments to the syringe and for each channel Finally attach a tube to tube connector to the C Flex tubing on the out board end of the valve and attach a short piece of Tygon tubing Insert a short length of PE 160 tubing into the Tygon tubing and make a connec tion to the manifold Overflow Sensor An overflow or spill sensor is has been provided to assist you in providing a level of protection to your microscope while using the VC 8 Valve Control System The overflow sensor itself is a Ag AgCl probe which grounds through the chamber bath A resistance of 10 MA or less between the probe and bath will trigger the overflow circuitry Place the probe adjacent your Publication 5713 001 REV A s Perfusion Valve Controller Model VC 8 Warner In
8. e Flow Adjustment Flow rates can be adjusted by raising or lowering the reservoir holder as well as by adjusting the height of each reservoir within the holder The table below lists the approximate flow rates for a reservoir at the spec ified height with the supplied Teflon tubing Reservoir height Approximate flow rate 61 cm 24 in 14 ml min mon 2n 20m Flow rates as a function of reservoir height Publication 5713 001 REV A Perfusion Valve Controller Model VC 8 Warner Instruments Maintenance Cleaning Do not use alcohol aromatic hydrocarbons or chlorinated solvents for cleaning They may adversely react with the plastic materials used to man ufacture the system If salt solution spills on the valve assembly it should be cleaned as soon as possible with a soft cloth dampened with a mild solu tion of detergent and water NOTE Teflon and Lee Mini Valves must be completely flushed with distilled water after each use Permanent damage will result if saline solution is allowed to crystallize inside the valve The exterior of the CONTROLLER may be cleaned periodically to remove dust grease and other contamination It is not necessary to clean the inside Use a soft cloth dampened with a mild solution of detergent and water Avoid abrasive cleaners Publication 5713 001 REV A 5 Perfusion Valve Controller Model VC 8 Warner Instruments Warranty The VC 8 is warranted to be free from defec
9. heme Applied Voltage V Valve Selection Event Out The Event Marker output produces a 500 ms logic level output 5 V each time a valve is turned on At all other times the Event Marker output is low OV Power Input Module A polarized 3 conductor IEC320 CEE 22 connector is used for line mains power input to the instrument A removable cordset terminated with a NEMA 5 15P connector is standard A fuse holder contains a protective fuse in series with the high side brown or black wire of the mains The holder accepts 5 x 20 mm fuses of the type indicated Publication 5713 001 REV A s Perfusion Valve Controller Model VC 8 Warner Instruments Control Description Conto AC Conversion ATTENTION PLEASE READ BEFORE APPLYING POWER TO YOUR UNIT The unit uses only one fuse the second one is sent as spare Follow these instructions to change the unit s from 120 VAC or 220 VAC Power Entry Module Fuse holder side view Push out drawer to access the Spare Fuse Il Active Fuse i A Carefully pry open the fuse holder from the inside Depending on AC Voltage being by using a small flat screwdriver used turn VAC selector switch A to 110 VAC or 220 VAC Replace fuse according to voltage being used For 120 VAC 0 50 Amp 5 x 20 mm Slow Blow For 220 VAC 0 25 Amp 5 x 20 mm Slow Blow Instructions for Us
10. ions not using Warner equipment The complete system includes the valve controller a valve bracket with valves connecting cable C Flex tubing an MP Series manifold a ring stand eight 60 cc syringes 25 feet of Tygon tubing and an assortment of tubing connectors A new and exciting feature to the VC 8 system is the Spill Sensor Probe This probe is used to detect the presence of an overflow condition and when activated it will automatically shut off all valves protecting your microscope optics The controller can independently regulate the function of up to eight valves Individual valves can be controlled via a manual switch an external analog signal or an external digital TTL signal An event marker pulse gen erated each time a valve is switched on is provided at the rear of the instru ment for recording into your acquisition system Valve transitions opened or closed occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions Valve options are pinch Teflon and Lee miniature types Pinch valves are sup plied as standard equipment They are the simplest from a maintenance standpoint since solutions do not come into contact with the valve body and the tubing is easily exchanged Pinch valves are dual acting with both nor mally open and normally closed sides If the valve input line is split using a Y connector solution can flow to waste while the
11. struments Operating Directions conta perfusion chamber such that the maximum solution height say just prior to an actual overflow is achieved when the solution contacts the tip of the probe Contact between the probe and your solution will activate the over flow circuitry and close all the valves The OVERFLOW SENSOR plugs into the OVERFLOW IN BNC on the rear of the controller Publication 5713 001 REV A 2 Perfusion Valve Controller Model VC 8 Warner Instruments Control Description Front Panel The front panel contains TTL inputs for each valve a 3 position toggle switch for manually setting the state of each valve and an LED displaying the active status for each valve There is also a 2 position toggle switch for selecting the command input mode a SPILL SENSOR indicator and a power switch with power on LED POWER 1 2 3 4 5 7 8 OVER FLOW j 5 j 3 gt EXTERNAL ON ON ON ON ON ON ON ON ANALOG 3 or or 3 orr 3 or A or or hor Mor GO EXTERNA EXT EXT EXT EXT EXT EXT EXT EXT DIGITAL INPUT1 INPUT2 INPUT3 INPUT4 INPUTS INPUT amp INPUT INPUTS p WARNER DIGITALINPUTS WG 8 VALVE CONTROLLER INSTRUMENTS TTL Inputs TTL inputs front panel BNC s and ON OFF TOGGLE SWITCHES are pro vided for all 8 channels of the VC 8 controller Operation of the front panel TTL inputs is selected by placing the 2 position COMMAND INPUT TOG GLE into external digital mode Use of these inputs can allo
12. tor for the VALVE SET and 4 BNC connections for OVERFLOW IN STANDBY IN EXTERNAL ANALOG IN and EVENT OUT valves Y CAUTION 38883388 X A NOT FOR CLINICAL WARNER USE ON HUMANS INSTRUMENTS 115 220 60 50 Hz 0 5 0 25A SlowBlow OVERFLOW STANOBY EXT ANALOG EVENT IN N N OUT 3 OOO O MADE IN USA D Connector A 15 pin D type female connector is used to connect the VALVE BRACK ET and valves to the CONTROLLER Overflow In This BNC is used to connect the OVERFLOW SENSOR to the controller Place the sensor into position and plug it into this BNC Standby In This BNC is provided to allow the placing of the VALVE CONTROLLER into standby mode Standby mode disables EXT ANALOG IN inputs and closes all valves Activation of this input is selected by placing the FRONT PANEL COM MAND input toggle into external analog mode and a TTL level high G to 5 V places the instrument in standby Publication 5713 001 REV A gt O gt o o o D o O e Gi gt ks 2 wn lt D a Warner Instruments Control Description Conto Ext Analog In This BNC is used to operate valves using analog voltage settings The volt age applied to this input selects the valve to open All other vales are closed Switching voltages closes the open valve and open the other Valve selec tions are overridden by the OVERFLOW SENSOR and the STANDBY BNC The table below indicates the selection sc
13. ts in materials and workman ship for a period of two years from the date of shipment If a failure occurs within this period we will repair or replace the faulty component s at our discretion This warranty does not cover failure or damage caused by phys ical abuse or electrical stress e g exceeding specified input limits Shipping charges to the factory are the customer s responsibility Return shipping of the repaired unit will be paid by Warner Instruments Publication 5713 001 REV A 3 Perfusion Valve Controller Model VC 8 Warner Instruments Appendix Valves 12 VDC 0 25 A to maintain pinch Tubing 1 32 ID x 3 32 OD tubing Valve Bracket Delrin mounts on 3 8 or 1 2 ring stand Connection Cable 2 4 meter 8 ft connecting cable terminated with quick disconnects on valve end and 15 pin male D type connector on controller end Reservoirs 60 cc capacity syringes Reservoir Holder Delrin Holds eight syringes with thumb screws for each reservoir Valve Controller front panel Power Two position on off with power on LED Input Select Two position selecting analog or digital inputs Switch Selection On manual Offor External External Input 5 V TTL compatible BNC Connector Overflow LED indicating overflow condition Valve Controller rear panel Overflow In BNC connection to OVERFLOW SENSOR Triggered when sensed resistance falls below 10 MQ Standby In BNC connection to activate standby mode TTL high closes
14. w the opening of more than one valve at the same time from a digital source such as your computer Placing the ON OFFTOGGLE SWITCH into the on position places the asso ciated valve into its open state A lit LED indicates the open state of the valve This setting is overridden by the overflow sensor Placing the ON OFF TOGGLE SWITCH into the off position places the valve into its closed state An unlit LED indicates the closed state of the valve Placing the ON OFF TOGGLE SWITCH into the ext position activates the associated BNC inputs A logic level low 0 V applied to the BNC places the associated valve into the closed state Correspondingly a logic level high 3 to 5 V places the associated valve into the open state A lit and an unlit LED indicates the open and closed states of the valve respectively These settings can be overridden by the overflow sensor Command Input Toggle The COMMAND INPUT TOGGLE is used to select between the front panel external digital controls and the rear panel external analog controls Publication 5713 001 REV A Perfusion Valve Controller Model VC 8 Warner Instruments Control Description Conto Overflow The OVERFLOW LED indicates an overflow condition sensed by the OVER FLOW SENSOR Activation of this circuit by an overflow condition immedi ately overrides all other settings and closes all valves Rear Panel The rear panel contains the power input module with fuse a 15 pin D con nec
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