Home

Agilent 6400 Series Triple Quad LC/MS System

Contents

1. E Recent Documents ii F Wrap Tet az Open E Merge amp Center A SaveAs gt A Prepa gt F 7 Publish p Eee omone ereen Figure 24 Excel Options button d Click Trust Center in the navigation pane e Click Trust Center Settings See Figure 25 Agilent 6400 Series Triple Quad LC MS System Installation Guide 49 2 2 Software Installation and Configuration Step 7 Install and configure Microsoft Excel 2007 Excel Options Popular Help keep your documents safe and your computer secure and healthy Formulas Proofing Protecting your privacy Save Microsoft cares about your privacy For more information about how Microsoft Office Excel helps to protect your privacy please see the privacy statements Advanced how the Microsoft Office Excel pri statemen Customize Microsoft Offi nline priva atemen mer Experience Improvement Program Add Ins E a Resources Learn more about protecting your privacy and security from Microsoft Office Online Microsoft Window ri nter Microsoft Trustworth mputin Microsoft Office Excel Trust Center The Trust Center contains security and privacy settings These settings help keep your computer secure We recommend that Trust Center Settings you do not change these settings i Figure 25 Excel Options window f Make sure that the Trust access to the VBA project object model check box is marked Trust Center Trusted Publishers Macro Settings
2. To view the high vacuum gauge reading click the Cell tab in the Tune Context and check that the high vacuum gauge reading is within range If not see To reset the Collision Cell gas flow rate on page 77 2 Start the Data Acquisition program change the Context to Tune and start an Autotune After the autotune has completed you may need to wait up to 30 minutes to allow for the calibrant solution to be pumped out of the Triple Quad This minimizes any background signal attributable to the calibrant In addition you can sonicate the nebulizer in a small graduated cylinder filled with acetonitrile for 10 minutes 3 Change the Context to Acquisition click File gt Load and then load the method MMI ES Pos MRM Reserpine Checkout m The checkout method includes the following acquisition parameters 2 ul injection isocratic from channel A at 0 4 mL min 1 minute run time 250 C Drying gas temperature 5 L min Drying gas flow can be optimized typical 10 to 13 L minutes 60 psi nebulizer pressure can be optimized typical 35 to 40 psi 2000 V capillary voltage 250 C Vaporizer temperature 2000 V charge voltage 37 V collision cell energy can be optimized typical between 37 to 41 V 240 V fragmentor voltage can be optimized typical between 140 to 250 V 400 V Delta EMV can be optimized typical between 200 to 400 V starting with Acquisition program version B 02 01 MS1 Resolution set to Wide and MS2 Resolution
3. Chonsiogan Twp Label Peounorln Poan ia Vane Mam feomtss fosa figs 10 som of er Figure 47 MS Q00 Chromatogram tab Agilent 6400 Series Triple Quad LC MS System Installation Guide 109 110 Reference 6460 6430 Reserpine Checkout Method Acquisition Parameters Agilent 6400 Series Triple Quad LC MS System Installation Guide www agilent com In This Book This book contains installation configuration verification and start up tasks to operate your Triple Quad LC MS System Agilent Technologies Inc 2006 2010 Tenth Edition May 2010 G3335 90081 Revision B ee Agilent Technologies
4. Method Items E Sy Signal definition x M Noise measurement Noise definition RMS vyiAX 1 0 Noise regions fot 0 0 350 A min Enter time ranges separated by commas For example 1 2 4 5 J Automatically compute when chromatograms are integrated Figure 33 Calculate Signal to Noise dialog box 4 Generate a printout of each signal to noise calculation report for each solvent blank and reserpine injection Include the chromatogram in the printout 5 Open the Excel spreadsheet D MassHunter Support Checkout Sensitivity Checkout Report xls Fill in the values to calculate the average signal to noise and save the spreadsheet The average signal to noise must be greater than that listed in Table 4 Table 4 Minimum average signal to noise Instrument Model Source Average Signal to Noise 1x RMS 6460 ESI with Agilent Jet Stream Technology 1000 1 6430 ESI 300 1 6410 ESI 150 1 6410 Multimode 150 1 6 Generate a printout of the Excel signal to noise report Agilent 6400 Series Triple Quad LC MS System Installation Guide 75 3 installation Completion and System Verification S N B c a oe E 1 16460 Triple Quad LC MS Sensitivity Checkout Report 2 3 4 4 Instrument Serial Number US83310889 5 6 Date 28 Aug 2008 7 8 9 10 Signal to Noise 11 Blank 1 0 27 12 Blank 2 0 38 13 Blank 3 0 42 14 Blank 4 0 3 15 Blank 5 0 41 16 Blank Ave
5. connect the nitrogen gas source to the center connector of one T union Connect two 10 cm lengths of 1 4 inch teflon gas tubing to each side of the T union Use the nuts and ferrules supplied with the T union Attach the other end of the 10 cm lengths of 1 4 inch teflon gas tubings from step c to the two BMT 4 gas filters Use the nuts and ferrules supplied with the BMT 4 gas filters Use a small length of gas tubings to connect each BMT 4 gas filter to a BHT 4 gas filter Use the nuts and ferrules from the BMT 4 and BHT 4 gas filters Attach the other end of each BHT 4 gas filter to a 10 cm length of 1 4 inc teflon gas tubing Use the nuts and ferrules supplied with the BHT 4 gas filters Outlet to LC MS BHT 4 BMT 4 Inlet from nitrogen gas source Figure 10 Gas filter con nected first in series then in parallel Connect the other end of each 10 cm length of gas tubing from step f to the ends of the remaining T union Use the nuts and ferrules supplied with the T union Connect the gas tubing from the BHT 4 gas filter from step g to the nitrogen gas fitting on the back of the LC MS system Use the supplied nut and ferrule kit p n 5183 0393 Secure the gas filter in a vertical position with tie wraps Agilent 6400 Series Triple Quad LC MS System Installation Guide 1 20 Instrument Installation Step 4 Connect the Triple Quad instrument 1 8 inch nitrogen gas connection for collision g
6. e0 e0e 9209 E T This chapter contains acquisition parameters for the HPLC Chip and reserpine checkout method Agilent Technologies 105 6 Reference pine Checkout Method Acquisition Parameters 6460 6430 Reserpine Checkout Method Acquisition Parameters The following graphics give the default acquisition parameters for the 6460 and 6430 POS MRM Reserpine Checkout method Sample Properties hALS SL BinPump SL Column SL DAD SL MSQQQ Method Path D MassHunter methods 6460 ESI_ AJT Pos MRM Checkout m Pre run Script E Post un Script E Description 6460 Triple Quad LC MS ESI with Agilent Jet Stream TechnologylPositive MRM Reserpine Checkout N Figure 41 Properties tab Sample Properties h ALS SL BinPump SL Column SL DAD SL MSQQQ Options Carryover Reduction Injection High Throughput Standard injection Disable overlapped injection Injection with Needle Wash C Start overlap when sample is flushed out C Use Injector Program Total lines C Start overlap after p a minutes Injection Volume 2 a ul Automatic delay volume reduction Needle Wash Wash needle in wash vessel and repeat a times Wash needle in Flush Portfor 10 seconds Figure 42 ALS tab 106 Agilent 6400 Series Triple Quad LC MS System Installation Guide Reference 6 6460 6430 Reserpine Checkout Method Acquisition Parameters Figure 43 Bin Pump tab Fig
7. is 100 1 8x RMS noise Agilent 6400 Series Triple Quad LC MS System Installation Guide 93 4 Installation and Verification of Other Sources Step 3 Analyze the results A Chromatogram Results ive tiQhwvGlaioc gt ALE A A R BH minutes X TIC MAM gt chip_100amol_3 d x102 Noise RMS 0 52 SNR 3 720min 210 2 3 70 0 MAM 575 50000 gt 694 49844 chip_100amol_3 d x101 Noise RMS 0 30 SNR 3 710min 69 1 1 MRM 575 50000 gt 823 39844 chip_100amol_3 d 101 Noise RMS 0 36 SNR 3 721 min 36 2 3721 0 MAM 575 50000 gt 937 50000 chip_100amol_3 d x101 Noise RMS 0 24 SNR 3 719min 320 0 39 0 0 5 1 1 5 2 25 3 3 5 4 45 55 6 5 7 ta Counts vs Acquisition Time min Figure 36 Calculated Signal to noise for 575 5 to 937 5 Transition 94 Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation and Verification of Other Sources 4 Step 3 Analyze the results LS 37 50000 694 39844 823 39844 Figure 37 Mass Spectra for 575 5 m z Agilent 6400 Series Triple Quad LC MS System Installation Guide 95 4 Installation and Verification of Other Sources Step 3 Analyze the results 96 Agilent 6400 Series Triple Quad LC MS System Installation Guide Agilent 6400 Series Triple Quad LC MS System Installation Guide 5 Other Setup Tasks To start the Data Acquisition program 98 To stop MassHunter processes in
8. the user guides to the location of your choice The installation program automatically copies the MassHunter user guides onto your computer so that they can be opened from online Help The user guides are also available on the installation disk in the Manuals folder Click this button only if you want an additional set of user guides copied to the location of your choice Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration 2 Step 5 Install the Qualitative Analysis program The Qualitative Analysis and Quantitative Analysis programs are pre installed on the shipping PC when ordered together with the instrument as a bundle If the programs are already installed continue with Step 7 Install and configure Microsoft Excel 2007 on page 47 1 Check that no other program is running on your system including Windows Update Installation of the Qualitative Analysis program will fail if Windows Update is running even in the background 2 Install the Qualitative Analysis program a b Put the Qualitative Analysis installation DVD into the DVD drive If you are installing only the service pack for the Qualitative Analysis program continue at step 4 From the installation disk double click Setup exe In the License Agreement screen click I accept the terms of the license agreement then click Next In the Choose Destination Location screen click Next to accept the defaul
9. 11 10 fmol ul HSA P1 F7 43mm 9min hsa mrm 575 unitm 10fmol 1002 d Calibration 3 1 12 10 fmolful HSA P1 F 43mm Smin hse mrm 575 unitm 10fmol 1003 d Calibration 3 1 13 100 fmol ul HSA P1 F8 43mm Smin hse mrm 575 unit m 100fmol r001 d Calibration 4 1 14 100 fmol ul HSA P1 F8 43rmm Smin hse mrm 575 unitm 100fmoH002 d Calibration 4 j1 15 1100 fmol ul HSA P1 F8 43rm Smin hse mem 575 unitm 100fmol r003 d Calibration 4 1 Cl Figure 34 HPLC Chip MS Worklist 90 Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation and Verification of Other Sources 4 2 Create the QQQ acquisition method as shown below Nanoflow Pump Solvents Flow Max pressure Stop time Post time Gradient Capillary Pump Solvent Flow Max pressure Al 3 97 acetonitrile water with 0 1 formic acid B1 90 10 acetonitrile water with 0 1 formic acid 600 nL min with fast gradient solvent consumption 175 bar 9 minutes 0 minutes Time B 0 3 0 7 70 0 7 1 3 A1 97 3 water acetonitrile with 0 1 formic acid 4 00 uL min primary flow 200 uL min 175 bar Micro wellplate sampler Injection volume Needle wash Needle flush solvent Bottom sensing Vial offset ALS Therm 1 uL enabled for 5 sec in flushport 20 80 methanol water with 0 1 formic acid On 0 On 40 C HPLC Chip MS Interface Injection flush volume 4 uL Pumps Chip Cube Timetable QQQ MRM Method Ionization mode Time filter
10. 84 To install the G1978B multimode source 84 To verify the operation of the G1978B multimode source 85 G1971B APPI Source 86 To install the G1971B APPI source 86 To verify the operation of the G1971B APPI source 87 HPLC Chip MS Cube Interface 88 Step 1 Prepare performance verification sample 88 Step 2 Prepare the HPLC Chip MS 90 Step 3 Analyze the results 93 5 Other Setup Tasks 97 To start the Data Acquisition program 98 To stop MassHunter Engines 98 To stop MassHunter processes in case of unrecoverable errors 98 To view the logbook 98 To configure the network interface card 99 To confirm configuration of NICs 100 To remove the MassHunter Workstation software 102 To reconfigure the instrument 103 6 Reference 105 6460 6430 Reserpine Checkout Method Acquisition Parameters 106 6 Agilent 6400 Series Triple Quad LC MS System Installation Guide Agilent 6400 Series Triple Quad LC MS System Installation Guide ee 1 7 e Instrument Installation 8 e e a e g Step 1 Unpack and set up the Triple Quad instrument 8 Step 2 Prepare to install the system 9 Step 3 Set up the rough pump 13 Step 4 Connect the Triple Quad instrument 16 Step 5 Install the source 23 Step 6 Install the Agilent 1100 or 1200 series LC system 29 This chapter describes the steps that are needed to install the Triple Quad instrument a Figure 1 6460 Triple Quad LC MS wN mnn ee Agilent Technologies 1 Instrument Instal
11. G1947B APCI Source 82 To install the G1947B APCI source 1 Install these parts from the APCI enablement kits that ship with the G1947B APCI source into the Aux module on the Triple Quad instrument APCI High Voltage Power Supply p n G1946 80058 Pour the Electrospray calibrant back into its original bottle or another suitable container rinse the calibrant bottle with acetonitrile pour the APCI calibrant into the calibrant bottle and attach the calibrant bottle back onto the CDS Remove the electrospray source and install the APCI source onto the spray chamber mounts Connect the vaporizer heater and APCI corona needle cables to the respective connectors on the left side panel of the Triple Quad instrument Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation and Verification of Other Sources 4 To verify the operation of the G1947B APCI source There is no sensitivity checkout with the G1947B APCI source on the Triple Quad instrument To verify its proper operation you need to run a check tune 1 Check the tuning of the APCI source a b In the Context list click Tune in the Data Acquisition program Load the autotune file that was generated with the electrospray or multimode source In the Manual Tune tab mark the Calibrant check box then acquire the data Verify that the masses in the MS1 and MS2 tabs are correct for the loaded source before you manually tune the peaks Verify th
12. MassHunter Data Acquisition program 98 Agilent 6400 Series Triple Quad LC MS System Installation Guide Other Setup Tasks 5 To configure the network interface card The PC shipped with the Triple Quad instrument is equipped with two network cards LAN cards The first LAN card is for connection to the house network via 10 100 1000 Base T network card The IP address for this card can either be static or dynamically assigned e g obtained by a DHCP server By default TCP IP is configured as the network protocol for this card which is built in to the PC The second LAN card in slot 1 is dedicated for instrument communication with the Triple Quad instrument and the Agilent 1100 1200 Series LC system This network card should NOT be connected to the site LAN To verify is the Instrument LAN network card settings are correct follow these steps 1 Click Start gt Settings gt Control Panel 2 Double click Network Connections 3 Right click the icon for the network card for the house LAN click Properties then configure the network card as needed Because this network card is independent from the card used for instrument control it may be configured using a static IP address or using a DHCP server 4 Right click the icon for the network card for the Triple Quad instrument click Properties then check that these parameters are set Subnet Mask 255 255 255 0 Gateway leave blank Because network configurati
13. Masshunter Reporting Quant Ll Solver Add in Cancel Masshunter Addin Loader Agilent 6400 Series Triple Quad LC MS System Installation Guide All MassHunter add in check boxes marked Software Installation and Configuration 2 Step 11 Configure the instrument In this step you configure the Triple Quad instrument and the 1100 1200 LC 1 Open the Agilent MassHunter folder on the desktop then double click Acq Tools 2 Double click the Instrument Configuration tool 3 In the Instrument Configuration dialog box a If you want to change the name of the instrument type a new name for Instrument Name b Click Agilent 6400 Series Triple Quadrupole c Mark the Agilent 1100 1200 1290 System Access check box d Click Configure E Instrument Configuration Instrument Name Instrument 1 Mass Spectrometer Agilent 6400 Series Triple Quadrupole 192 168 254 12 C Agilent 1100 1200 System Access Agilent 35900E A D Converter Figure 28 Instrument Configuration Dialog Box 4 When configuration is completed click OK Agilent 6400 Series Triple Quad LC MS System Installation Guide 55 2 Software Installation and Configuration Step 12 Copy the support folder to the computer In this step you copy the Support folder from the Data Acquisition installation disk to the D MassHunter folder 1 In a Windows Explorer window open the installation disk drive 2 Right click the Support folder and click Copy 3 Right cl
14. S N 0 17 18 Area Height 19 Reserpine 1 1207 2 924 00 404 00 20 Reserpine 2 1368 5 973 00 419 00 21 Reserpine 3 1583 7 959 00 412 00 22 Reserpine 4 1132 6 1044 00 448 00 23 Reserpine 5 2086 1091 00 471 00 24 Reserpine Ave S N 1476 998 20 430 80 AVE 25 67 80 27 94 STD 26 6 79 6 49 RSD 27 28 SignaltoNoise _1475 1 29 Reserpine Ave Blank Ave PASS If verification in negative mode is required verify the instrument with the parameters listed in Table 5 Use the Agilent ES Negative Ion Performance Standard p n G1946 85005 that is included with your instrument Table5 Electrospray Negative Mode Sample Acid Red 4 10 ng pl in 50 50 water IPA Concentration after dilution 1 pg ul Injection volume 1 pl Total sample amount injected 1pg Sample order number G1946 85005 Solvents 50 50 isocratic 100 Water no modifiers 100 Acetonitrile no modifiers Method ESI Neg MRM Acid Red 4 Checkout m Worklist ESI Neg MRM Acid Red 4 Checkout wkl 76 Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation Completion and System Verification 3 To reset the Collision Cell gas flow rate Most parameters like the Collision Cell gas flow rate should work with values set at the factory In case the default flow rate does not give a high vacuum gauge reading in the range of 1 9 to 2 3 x 10e for a 6460 6430 and 2 7 to 3 3 x 10 for 6410 you will need to do two steps De
15. Step 7 Install and configure Microsoft Excel 2007 47 Step 8 Install Service Packs for Data Acquisition 51 Step 9 Install Quantitative Analysis Reporting 52 Step 10 Check Excel Add Ins for MassHunter 54 Step 11 Configure the instrument 55 Step 12 Copy the support folder to the computer 56 Step 13 Confirm the LC firmware revision 58 Step 14 Finish the installation 59 If you purchased a bundled Triple Quad instrument system the MassHunter Workstation software has been installed for you In this case skip to Step 7 Install and configure Microsoft Excel 2007 and complete the steps that remain ee Agilent Technologies 31 2 Software Installation and Configuration Step 1 Set up the computer system 1 8 Set up the PC a Verify that the line voltage is correct b Unpack the PC and put it on a suitable benchtop or table c Connect the keyboard mouse monitor and printer to the computer Verify that two network cards are available in the PC One of these is usually a built in card and the second is located in an accessory slot Follow the installation documentation supplied with the PC to install and verify components as needed Install the network hub a Put the hub on the table next to the PC b Plug the power supply into the hub c Connect the power supply to a power outlet Connect one end of a Category 5 shielded twisted pair STP cable to any port 1 to 7 on the network hub Connect the other en
16. Table 1 6430 and 6460 Performance Verification Summary MRM Mode 6460 ESI with Agilent Jet Stream Technology Positive MRM Mode 6430 6460 ES G1948B Positive MRM Mode Sample Reserpine 5 ng pL Concentration after 500 fg pL dilution Injection volume 2uL Total sample amount 1 pg injected Sample order number p n G2423A Solvent 70 30 acetonitrile water Method 6460 ESI_AJT Pos MRM Checkout m Worklist 6460 ESI_AJT Pos MRM Checkout wkl Performance 1000 1 1x RMS specifications Reserpine 5 ng pL 500 fg pL 2 uL 1 pg G2423A 70 30 acetonitrile water 6400 ESI Pos MRM Checkout m 6400 ESI Pos MRM Checkout wkl 300 1 1 x RMS Table 2 6410 Performance Verification Summary MRM Mode ES G1948B Positive MRM Mode ES G1978B Positive MRM Mode Sample Reserpine 5 ng pL Concentration after dilution 500 fg pL Injection volume 2 ul Total sample amount injected 1 pg Sample order number p n G2423A Solvent 70 30 acetonitrile water Method 6400 ESI Pos MRM Checkout m Worklist 6400 ESI Pos MRM Checkout wkl Performance specifications 150 1 1 x RMS Reserpine 5 ng pL 500 fg pL 2uL 1 pg G2423A 70 30 acetonitrile water MMI ES Pos MRM Reserpine Checkout m MMI ES Pos Reserpine Checkout wkl 150 1 1 x RMS Agilent 6400 Series Triple Quad LC MS System Installation Guide 65 3 Installation Completion and System Verification Step 3 Review methods and run worklist Before you begin cop
17. disk then for each service pack file Double click the name of the most recent service pack to run the installation program You may need to open folders inside the service pack folder to find the installation file The name of service pack installation files typically end in SP x exe Follow the instructions to install the service pack Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration 2 Step 6 Install the Quantitative Analysis program 1 Check that no other program is running on your system Installation of the Quantitative Analysis program will fail if Windows Update is running even in the background 2 Install the Quantitative Analysis program a b Put the Quantitative Analysis installation disk into the disk drive If you are installing only the service pack for the Quantitative Analysis program continue at step 5 From the root directory of the installation disk double click setup_quant exe When prompted select the language for the installation In the Welcome to the InstallShield Wizard window click Next to continue with the installation In the License Agreement window click I accept the terms in the license agreement then click Next In the Destination Folder window click Next to accept the default destination location The default is C Program Files Agilent MassHunter Workstation Quant In the Customer Data Folder window click the C
18. in the Adaptors and Bindings tab the LAN card that is communicating to the Triple Quad instrument is listed first If it is not select the card click the arrow on the right and move it to the top Then click OK to apply the setting Agilent 6400 Series Triple Quad LC MS System Installation Guide 101 5 Other Setup Tasks To remove the MassHunter Workstation software 1 If you have any data methods libraries or other files or directories that you want to save move or copy them to a directory outside of those to be deleted 2 Click Start gt Settings gt Control Panel then double click Add or Remove Programs 3 Remove these programs Agilent MassHunter WorkStation Data Acquisition Agilent MassHunter WorkStation Qualitative Analysis Agilent MassHunter WorkStation Quantitative Analysis 4 Close all applications except Explorer Reboot the computer You are now ready to load new software 102 Agilent 6400 Series Triple Quad LC MS System Installation Guide Other Setup Tasks 5 To reconfigure the instrument If you have more than one stack of HPLC modules from which to choose or a new supported LC module is installed the Instrument Configuration registry must be cleared for MassHunter Workstation 1 Put the Triple Quad instrument into standby mode 2 Close the MassHunter Acquisition program 3 Stop MassHunter processes See To stop MassHunter processes in case of unrecoverable errors on page 98 4 Start the Instru
19. in the range of 1 9 to 2 3 x 10e torr To view the high vacuum gauge reading click the Cell tab in the Tune Context and check that the high vacuum gauge reading is within range If not see To reset the Collision Cell gas flow rate on page 77 2 Start the Data Acquisition program change the Context to Tune and start an Autotune After the autotune has completed you may need to wait up to 30 minutes to allow for the calibrant solution to be pumped out of the Triple Quad This minimizes any background signal attributable to the calibrant In addition you can sonicate the nebulizer in a small graduated cylinder filled with acetonitrile for 10 minutes Parameters in the method can be modified by your Agilent representative for optimal response 3 Change the Context to Acquisition click File gt Load and then load the method 6400 ESI Pos MRM Checkout m The checkout method includes the following acquisition parameters 2 uL injection isocratic from channel A and B 70 30 at 0 4 mL minute 1 minutes run time 325 C Drying gas temperature 10 L minute Drying gas flow can be optimized typical 10 to 13 L minute 35 psi nebulizer pressure can be optimized typical 20 to 35 psi 4000 V capillary voltage 37 V collision cell energy can be optimized typical between 37 to 41 V 240 V fragmentor voltage can be optimized typical between 140 to 250 V 200 V Delta EMV can be optimized typical between 200 to 400 V MS1 Resol
20. system Step 3 Setup the rough pump 13 Step 4 Connect the Triple Quad instrument 16 Step 5 Install the source 23 Step 6 Install the Agilent 1100 or 1200 series LC system 29 2 Software Installation and Configuration Step 1 Set up the computer system 31 32 Step 2 Check PC network card configuration 33 Step 3 Prepare for installation 35 Step 4 Install the Data Acquisition program 36 Step 5 Install the Qualitative Analysis program 41 Step 6 Install the Quantitative Analysis program 43 Step 7 Install and configure Microsoft Excel 2007 47 Step 8 Install Service Packs for Data Acquisition 51 Step 9 Install Quantitative Analysis Reporting 52 Step 10 Check Excel Add Ins for MassHunter 54 Step 11 Configure the instrument 55 Step 12 Copy the support folder to the computer 56 Step 13 Confirm the LC firmware revision 58 Step 14 Finish the installation 59 3 Installation Completion and System Verification 61 Step 1 Condition the 1100 1200 LC 62 Step 2 Prepare the performance evaluation samples 64 Step 3 Review methods and run worklist Step 4 Verify the Triple Quad sensitivity To reset the Collision Cell gas flow rate Agilent 6400 Series Triple Quad LC MS System Installation Guide 66 73 71 Contents 4 Installation and Verification of Other Sources 81 G1947B APCI Source 82 To install the G1947B APCl source 82 To verify the operation of the G1947B APCl source 83 G1978B Multimode Source
21. the supplied bottle Always rinse the graduated pipettes and volumetric flasks thoroughly with deionized water before and between each use Use polypropylene labware for preparing performance evaluation samples since glass vessels introduce unacceptable levels of sodium Always rinse the autosampler vials and caps with the solvent mix used for sample dilution before filling them with the performance verification samples This minimizes any background that can be contributed by the vials and caps The vials may be run uncapped if the septa are found to be a source of background contamination Before you begin be sure that you have the following 64 1 mL graduated pipette p n 9301 1423 2 x 100 mL volumetric flasks p n 9301 1344 Positive mode performance evaluation sample Agilent G2423A Plastic bottle for storing first dilution p n 9301 1433 Transfer 1 mL of 5 ng uL reserpine p n G2423A to a 100 mL volumetric flask Use a clean graduated pipette Dilute to the 100 mL mark with 70 30 acetonitrile water Transfer 1 mL of the first dilution to a 100 mL volumetric flask Use a clean graduated pipette Dilute to the 100 mL mark with 70 30 acetonitrile water This provides 500 fg uL at 2 uL injection volume which equals the final 1 pg uL reserpine concentration that is needed for performance verification Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation Completion and System Verification 3
22. 0 Series Triple Quad LC MS System Installation Guide 11 1 Instrument Installation Step 2 Prepare to install the system e Take an inventory of the contents of the packages f Record serial numbers The serial number label for the Triple Quad instrument is located on the lower left corner to the left of the instrument power push button 2 Use four people one at each corner of the instrument to lift the Triple Quad instrument onto a stable bench or table NOTE Do NOT lift from under the power switch bracket on the front left corner of the instrument The bracket will bend See Figure 6 Figure 6 Do not lift from under power switch bracket WARNING Do not lift the Triple Quad without assistance The instrument weighs up to 115 kg 255 Ibs Lift with your knees not with your back Keep your back straight while you bend your knees 12 Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 Step 3 Set up the rough pump 1 Unpack the mist filter exhaust hose and pump oil drip pan from the shipping kit 2 Remove the rough pump from its shipping container a Cut the straps that secure the cardboard box to the wooden pallet b Lift off the cardboard box c Set aside the two bottles of pump oil and funnel d Set aside the oil return kit The felt plugs and screen inside the packaging are not needed and may be discarded e Use a pair of needle nose pliers to remove the metal clips tha
23. 1 Never use aliphatic aromatic or halogenated hydrocarbons in the CDS These solvents are not compatible with the O ring in the CDS 10 Turn on the Triple Quad front power switch to start the pump down of the Triple Quad The rough pump will become quieter within a few seconds G1978B Multimode Source 1 Install these parts from the Multimode and APCI enablement kits that ship with the G1978B Multimode source into the Aux module on the Triple Quad LC MS APCI High Voltage Power Supply p n G1946 80058 Multimode HV board p n G1960 61015 Valve board APCI HV PS cable p n G1960 60802 Valve board APCI Needle Interlock cable p n G1960 60856 Multimode HV board cable p n G1960 60858 Multimode Power Data cable p n G1960 60878 MMI L Low Concentration Tuning Mix p n G1969 85020 Connect the multimode and corona needle cables to the respective connectors on the left side panel of the Triple Quad LC MS Put the nebulizer in the nebulizer adjustment fixture supplied in the shipping kit and check that the nebulizer needle is properly adjusted The nebulizer needle should be even with the end of the nebulizer nozzle 4 Install the nebulizer in the spray chamber Install the spray chamber on the spray chamber mount close the spray chamber and fasten the latch You may need to adjust the latch to ensure that the O ring seals completely Use a 14 inch x 5 16 inch wrench to loosen the lock nut then adjust the l
24. 80078 to nitrogen gas source Use the supplied nut and ferrule kit p n 5183 0392 Keep some tubing for step c and step d Attach the other end of the of the tubing from the nitrogen gas source to the BHT 4 gas filter Use the nuts and ferrules supplied with the BHT 4 gas filter Use a piece of 1 4 inch teflon gas tubing to connect the BMT 4 gas filter to the BHT 4 gas filter Use the nuts and ferrules supplied with the BMT 4 and BHT 4 gas filters Connect a new length of 1 4 inch teflon gas tubing to the other end of the BHT 4 gas filter Use the other nut and ferrule kit supplied with the BHT 4 gas filter Connect the gas tubing from the BHT 4 gas filter from step d to the nitrogen gas fitting on the back of the LC MS system Use the supplied nut and ferrule kit p n 5183 0392 Secure the gas filter in a vertical position with tie wraps This configures the filters in series with the supply tubing See Figure 9 as an example for assembling the gas trap in the correct configuration Outlet to LC MS BHT 4 BMT 4 Inlet from nitrogen gas source Figure 9 Gas filter con nected in series Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 Step 4 Connect the Triple Quad instrument For 6460 Locate the 2 1 4 inch brass T unions 0100 0088 and loosen the brass nuts on the T unions Use an appropriate length of the 1 4 inch teflon gas tubing G1946 80078 to
25. Agilent 6400 Series Triple Quad LC MS System Installation Guide Ee Agilent Technologies Notices Agilent Technologies Inc 2006 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 63335 90081 Edition Tenth Edition May 2010 Printed in USA Agilent Technologies Inc 5301 Stevens Creek Blvd Santa Clara CA 95051 Windows and Microsoft is a U S regis tered trademark of Microsoft Corporation Windows NT is a U S registered trade mark of Microsoft Corporation Software Revision This guide is valid for the B 03 01 revision or higher of the Agilent MassHunter Worksta tion Software Data Acquisition program and compatible Qualitative Analysis and Quantitative Analysis programs until super seded Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular
26. Data Acquisition Software then follow the instructions on the screen to install the software In the License Agreement screen click I accept the terms of the license agreement then click Next In the Choose Destination Location screen click Next to accept the default destination location The default is C Program Files Agilent MassHunter Workstation Acq In the Choose location for customer data files screen check that the default of D MassHunter is displayed in the text box then click Next See Figure 18 Agilent MassHunter Workstation Installation Choose location for customer data files Agilent Technologies Select a folder for customer specific files like data files and methods You can type a new folder or select a new folder with the Browse button Click Next when the folder has been selected D MassHunter Browse lt Back Cancel Figure 18 Choose location for customer data files screen gt In the Start Copying Files screen review the settings then click Next The Setup Status screen now appears gt If you get a message that indicates that the screen resolution is not set to the required 1280 x 1024 resolution click OK The best resolution for MassHunter is either 1600 x 1200 for 4 x 3 aspect ratio or 1920 x 1080 for 16 x 9 Agilent 6400 Series Triple Quad LC MS System Installation Guide 37 2 38 Software Installation and Configuration If you see a Notepad window that prompts
27. G2453 85050 5 M formic acid p n G2453 85060 Start up the Data Acquisition program Condition the LC as specified in its installation document Disconnect any column s that may be installed Rinse the LC solvent bottles three times with hot 60 C water then rinse them three times with methanol Add the 500 mL of flushing solvent p n G1969 85026 to a clean solvent bottle and connect it to channel A Set up the pump module to deliver the flushing solvent for 15 minutes at 3 mL minute Set up the method for LC only acquisition Do 5 injections with the flushing solvent to clean the injector path and sample loop Flush out system overnight with the flushing solvent a Set up the pump module for 0 5 mL minute flow with the flushing solvent Set the drying gas flow to 10 L minute Set the nebulizer pressure to 40 psi Set the drying gas temperature to 350 C For 6460 Set sheath gas temperature to 350 C For 6460 Set sheath gas flow to 10 L minute Move the flushing solvent into the Triple Quad spray chamber by right clicking on the MS QQQ icon and clicking LC gt MS in the Data Acquisition program oo a oOo S h Flush the system overnight with the flushing solvent Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation Completion and System Verification 3 8 After flushing overnight with the flushing solvent put 100 isopropyl alcohol in a clean solvent bottle and connect it to channe
28. Hunter Workstation software supports Excel 2007 or Office 2007 1 Close all MassHunter Workstation programs 2 Click Start gt All Programs gt Agilent gt MassHunter Workstation gt Acq Tools gt Remove MassHunter Processes to make sure that all MassHunter processes are stopped 3 Start the installation a Follow the instructions that came with Excel 2007 or Office 2007 to start the installation b If you are prompted to enter the Product Key do so If you do not enter the product key now Excel 2007 will remind you later which may interfere with report automation c If you are installing Excel 2007 or if you are installing Office 2007 and you want to install all Office options click Install Now Continue at step 5 d If you are installing Office 2007 and you want to customize your installation then click Customize 4 If Excel 2007 is already installed or you are doing a customized Office 2007 installation For Excel 2007 check that the disk icon next to Microsoft Office appears white If not right click Microsoft Office and click Run all from My Computer Then click Continue and continue the installation as instructed For Office 2007 check that the disk icon next to each of these programs appear white If not right click the program name and click Run all from My Computer Agilent 6400 Series Triple Quad LC MS System Installation Guide 47 2 Software Installation and Configuration Step 7 Install and configu
29. Quad mainframe to the sheath gas fitting on the ESI with Agilent Jet Stream Technology 10 Connect the PEEK tubing from the selection valve inside the front cover to the top of the nebulizer Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 Step 5 Install the source High voltage connection Vaporizer port Nebulizer gas Sheath gas Figure 14 Connections for ESI with Agilent Jet Stream Technology 11 Rinse the calibrant bottle three times with hot 60 C water and then three times with methanol Then rinse it once with acetonitrile 12 Add the ESI L Low Concentration Tuning Mix to the calibrant bottle Never use aliphatic aromatic or halogenated hydrocarbons in the CDS These solvents CAUTION f a are not compatible with the O ring in the CDS 13 Turn on the Triple Quad front power switch to start the pump down of the Triple Quad The rough pump will become quieter within a few seconds Agilent 6400 Series Triple Quad LC MS System Installation Guide 25 1 26 Instrument Installation Agilent G1948B Electrospray Before you begin check that you have these parts Agilent G1948B Electrospray interface the G1948A Electrospray interface is not supported Nebulizer spacer kit p n G1946 60254 ESI L Low Concentration Tuning Mix p n G1969 85000 Remove the foil that covers the spray chamber mount and remove the shipping cover from the Electrospray spray ch
30. Time Filter Width Dry gas flow Intelligent Sample Loading is activated Valve to enrichment at 7 5 min positive HPLC Chip on 0 07 5 L min Agilent 6400 Series Triple Quad LC MS System Installation Guide 91 4 92 Installation and Verification of Other Sources Step 2 Prepare the HPLC Chip MS Dry gas temp 325 C Capillary Voltage 1700 1800 V is typical Time segments 1 Delta EMV 0 V can go up to 200 V if more noise is desired MRM scan segments Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation and Verification of Other Sources 4 c gt A l4 step 3 Analyze the results Step 3 Analyze the results 1 In the Quantitative Analysis program create a batch and add the samples 2 Create a calibration curve and use the Curve Fit Assistant to optimize the curve to obtain a best fit that includes all points and optimizes Accuracy for the data points Calibration Curve i Type Quadratic m Origin Ignore 7 his jx oy Q Peptide 3 4 Levels 4 Levels Used 12 Points 12 Points Used 0 QCs x105 y 1 9858 x 2 1325 1144 x 35 7246 g R 2 0 99973531 Concentration fg uL Figure 35 HSA Calibration Curve Example You can use the Qualitative Analysis program to calculate the signal to noise ratio in Qualitative Analysis 3 For 100 amol uL in the 575 50 to 937 50 transition range check that the minimum signal to noise ratio defined from 0 5 to 3 0 minutes
31. Trusted Locations D R For macros in documents not in a trusted location Add ins Disable all macros without notification Disable all macros with notification ActiveX Settings Disable all macros except digitally signed macros C Enable all macros not recommended potentially dangerous code can run Message Bar Developer Macro Settings External Content M Trust access to th object model Privacy Options Figure 26 Mark the Trust access to the VBA project object model check box g Click OK in the Trust Center window h Click OK in the Excel Options window 50 Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration 2 Step 8 Install Service Packs for Data Acquisition 1 Put the Data Acquisition installation disk into the disk drive 2 From within a Windows file manager window check the installation disk for any folder that includes the word Service Packs 3 If one or more service pack files or folders exist on the installation disk then for each service pack file a Double click the name of the most recent service pack to run the installation program You may need to open folders inside the service pack folder to find the installation file The name of service pack installation files typically end in SP x exe b Follow the instructions to install the service pack Agilent 6400 Series Triple Quad LC MS System Installation Guide 51 2 52 Software Instal
32. Tuning Mix p n G1969 85000 1 Remove the top cover by loosening the T10 lock screw and lifting off the instrument chassis 2 Remove the electrospray interface with Agilent Jet Stream Technology from the packaging inside the instrument chassis next to the e Module See Figure 13 i Figure 13 Packaging for ESI with Agilent Jet Stream Technology 3 Remove the foil that covers the spray chamber mount and remove the shipping cover from the Electrospray spray chamber Agilent 6400 Series Triple Quad LC MS System Installation Guide 23 1 24 Instrument Installation Put the nebulizer in the nebulizer adjustment fixture supplied in the shipping kit and check that the nebulizer needle is properly adjusted The nebulizer needle should be even with the end of the nebulizer nozzle Install the nebulizer in the spray chamber Install the spray chamber on the spray chamber mount close the spray chamber and fasten the latch You may need to adjust the latch to ensure that the O ring seals completely Use a 14 inch x 5 16 inch wrench to loosen the lock nut then adjust the latch to the proper fit then tighten the lock nut so that the latch maintains its adjustment Connect the high voltage and vaporizer cables to the connectors on the left side panel of the instrument Connect the 1 8 inch nebulizing gas tubing from the Triple Quad mainframe to the nebulizer gas fitting Connect the 1 8 inch sheath gas tubing from the Triple
33. abled saan eh DS Autoconf iguration Enabled sa ate ees IP Address s s o es si o oo o 130 27 112 267 Subnet Mask gt gt gt aa aana a a 255 255 255 8 Default Gateway s a ao o a a a 130 22 112 1 DHCP Server 130 27 48 128 DNS Servers a aaaaa aa a a 130 27 48 128 1386 27 32 128 Primary WINS Server 130 29 154 21 Secondary WINS Server 130 27 152 33 Lease Obtained Wednesday September 17 2003 3 52 11 PM Lease Expires Wednesday September 24 2663 3 52 11 PM Figure 38 Example LAN Configuration 4 Check that the first Local Area Connection listed is the network card used to control the Triple Quad instrument 5 The default address for the LAN card should be 192 168 254 1 Default IP address 255 255 255 0 Subnet Mask Other Setup Tasks 5 6 Check that the second Local Area Connection listed contains the information for house LAN By default this connection comes configured for DHCP 7 If the network cards are not properly configured modify their settings a b Right click My Network Places then click Properties Right click the LAN card that is to be used to control the Triple Quad instrument then click Properties Double click the TCP IP protocol Configure the card using the IP addresses from above then click OK to apply the settings Click Advanced Scroll down to the Advanced Settings menu Check that
34. ainer rinse the calibrant bottle with acetonitrile pour the APCI APPI calibrant into the calibrant bottle and attach the calibrant bottle back onto the CDS Remove the currently installed source and install the APPI source onto the spray chamber mounts Connect the APPI vaporizer heater cable to its connector on the left side of the Triple Quad instrument Then connect the APPI serial cable to the USB Serial Converter cable that was installed in step 1 of this topic Connect the APPI power supply cable to the APPI DB9 power connector and screw it in This connector contains both serial and power interfaces Connect the other end of the serial cable to the Serial connector on the Smart card interface which is located on the left side of the instrument chassis Plug the APPI power supply into an AC outlet using the power cord supplied with the APPI interface kit Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation and Verification of Other Sources 4 To verify the operation of the G1971B APPI source There is no sensitivity checkout with the G1971B APPI source on the Triple Quad instrument To verify its proper operation you need to run a check tune to verify performance 1 Check the tuning of the APPI source a b In the Context list click Tune in the Data Acquisition program Load the autotune file that was generated with the electrospray or multimode source In the Manual Tune tab mark the Cal
35. alculate signal to noise for each injection a Load each solvent blank and reserpine sample data file into the Qualitative Analysis program b Generate Extracted Ion Chromatograms of the 195 1 ion Agilent 6400 Series Triple Quad LC MS System Installation Guide 73 3 Installation Completion and System Verification Uuad sensitivity ace Chromatogram definition Type eic O Integrate when ce extracted MS Chromatogram Advanced Excluded Masses MS level MS MS v Scans Multiple reaction monitor x m z value s 195 1 Do cycle sum C Merge multiple masses into one chromatogram Figure 32 Extract Chromatogram dialog box c Smooth the extracted chromatogram by setting Function to Quadratic Cubic Savitzky Golay and Function width to 11 points Smoothing function Quadratic Cubic avitzky Golay Function width i TA points Gaussian width points d Integrate each reserpine peak and click Calculate Signal to Noise e Calculate the signal to noise using Height f Under Noise Measurement for Noise definition select RMS and select X 1 g For Noise regions type 0 100 0 350 Make sure that the noise region does not include the reserpine peak 74 Agilent 6400 Series Triple Quad LC MS System Installation Guide M Signal measurement Installation Completion and System Verification E Method Editor Calculate SignaHo Notse AlI
36. amber Install the nebulizer spacer as follows a Use a flat bladed screwdriver to remove the two nebulizer shoulder screws from the top of the spray chamber b Use the two m3x8 Torx T10 screws to install the nebulizer spacer onto the top of the spray chamber c Install the two nebulizer shoulder screws into the top of the nebulizer spacer Put the nebulizer in the nebulizer adjustment fixture supplied in the shipping kit and check that the nebulizer needle is properly adjusted The nebulizer needle should be even with the end of the nebulizer nozzle 4 Install the nebulizer in the spray chamber Install the spray chamber on the spray chamber mount close the spray chamber and fasten the latch You may need to adjust the latch to ensure that the O ring seals completely Use a 14 inch x 5 16 inch wrench to loosen the lock nut then adjust the latch to the proper fit then tighten the lock nut so that the latch maintains its adjustment Connect the 1 8 inch nebulizing gas tubing from the Triple Quad mainframe to the nebulizer gas fitting Connect the PEEK tubing from the selection valve inside the front cover to the top of the nebulizer Rinse the calibrant bottle three times with hot 60 C water and then three times with methanol Then rinse it once with acetonitrile Add the Electrospray calibrant to the calibrant bottle Agilent 6400 Series Triple Quad LC MS System Installation Guide CAUTION Instrument Installation
37. ard into the LC detector module If a detector module is not present install the Agilent G1369A LAN Interface card into the pump module Before you install the LAN Interface card set switches 5 and 6 to the ON position This will force the Agilent LAN Interface Card to use its default IP address and subnet mask Note For a G1315C D DAD SL the LAN interface is built into the module main board To set the G1315C D to the proper IP address set switch positions 7 and 8 to the ON or UP position By default switch positions 7 and 8 are in the down position Refer to the Agilent 1200 Series Diode Array and Multiple Wavelength Detector User Manual part number G1315 90012 for more information The default IP address is 192 168 254 11 and the default subnet mask is 255 255 255 0 Agilent 6400 Series Triple Quad LC MS System Installation Guide 29 1 Instrument Installation JETTITE Do not use any other LAN interface card besides the G1369A e g the older Jet aia A Direct card J4100A 30 Agilent 6400 Series Triple Quad LC MS System Installation Guide Agilent 6400 Series Triple Quad LC MS System Installation Guide 2 Software Installation and Configuration Step 1 Set up the computer system 32 Step 2 Check PC network card configuration 33 Step 3 Prepare for installation 35 Step 4 Install the Data Acquisition program 36 Step 5 Install the Qualitative Analysis program 41 Step 6 Install the Quantitative Analysis program 43
38. as supply Do not use 1 4 inch nitrogen gas connection for drying gas supply Figure 11 Power and gas connections on back left of Triple Quad 8 Set the pressure on the nitrogen supply regulator at 80 to 100 psi 550 to 690 kPa Turn on the nitrogen gas for a few minutes to purge the tubing and gas conditioner before you connect the nitrogen gas supply to the Triple Quad Regulator pressures above 110 psi 760 kPa cause nitrogen waste due to release from the bleed valve on the flow control module Use the tubing cutters p n 8710 1709 supplied in the ship kit to cut an appropriate length of the 1 8 inch copper supply tubing p n 5180 4196 to connect from the outlet fitting on the collision cell nitrogen tank regulator to the nitrogen gas conditioner p n RMSN 2 for the collision cell gas then cut a second length of the 1 8 inch copper supply tubing to connect from the nitrogen gas conditioner to Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 Step 4 Connect the Triple Quad instrument the collision cell gas inlet fitting on the left side of the Triple Quad see Figure 11 Secure the gas filters in a vertical position with tie wraps Remote start connection LAN connection from HUB switch Rough pump power cord connection AC line power connection Figure 12 Power and electrical connection at back left side of Triple Quad 10 Set the pressure on the Collision Cell supp
39. at you have sufficient abundance for the tune peaks that the tune peaks have peak widths of 0 7 0 05 amu and that mass assignments are within 0 15 amu of the target mass assignments 2 Save the tune file Agilent 6400 Series Triple Quad LC MS System Installation Guide 83 4 Installation and Verification of Other Sources G1978B Multimode Source For more information on installing the multimode source please refer to the multimode user guide and set up guide To install the G1978B multimode source CAUTION If the multimode source is not already installed on your Triple Quad instrument do these steps 1 Install these parts from the Multimode and APCI enablement kits that ship with the G1978B Multimode source into the Aux module on the Triple Quad instrument For all models APCI High Voltage Power Supply p n G1946 80058 Multimode HV board cable p n G1960 60858 Do not install the G1960 65015 Multimode HV board on the 6460 or you may damage the ESI with Agilent Jet Stream Technology The Multimode HV board is already installed on the 6460 and is backward compatible with the G1978B multimode source 84 The Multimode Power Data cable is already installed on the 6460 Pour the electrospray tuning mix back into its original bottle or another suitable container rinse the calibrant bottle with acetonitrile pour the MMI L tuning mix into the calibrant bottle and attach the calibrant bottle back onto the CDS Rem
40. atch to the proper fit then tighten the lock nut so that the latch maintains its adjustment Connect the 1 8 inch nebulizing gas tubing from the Triple Quad mainframe to the nebulizer gas fitting Agilent 6400 Series Triple Quad LC MS System Installation Guide 27 1 Instrument Installation 7 Connect the PEEK tubing from the selection valve inside the front cover to the top of the nebulizer 8 Rinse the calibrant bottle three times with hot 60 C water and then three times with methanol Then rinse it once with acetonitrile 9 Add the Multimode calibrant to the calibrant bottle Never use aliphatic aromatic or halogenated hydrocarbons in the CDS These solvents CAUTION f oie are not compatible with the O ring in the CDS 10 Turn on the Triple Quad front power switch to start the pump down of the Triple Quad The rough pump will become quieter within a few seconds 28 Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 Step 6 Install the Agilent 1100 or 1200 series LC system Before you begin check that you have these parts e HPLC grade methanol or better e HPLC grade water or better 1 Install the Agilent 1100 or 1200 LC system Follow its installation documents Other LCs are not supported 2 Connect the remote start cable from the Triple Quad to a Remote Start connector on the LC sampler pump or other detector module only 3 Install an Agilent G1369A LAN Interface c
41. case of unrecoverable errors 98 To view the log book 98 To configure the network interface card 99 To confirm configuration of NICs 100 To remove the MassHunter Workstation software 102 To reconfigure the instrument 103 This chapter contains tasks that are related to setting up your Triple Quad instrument For complete information on using the your Triple Quad instrument refer to the list of resources found in the Quick Start Guide ott Agilent Technologies 97 5 Other Setup Tasks To start the Data Acquisition program You can start the Data Acquisition program in one of two ways e Double click the Data Acquisition icon in the Agilent MassHunter program group or e Click Start gt Programs gt Agilent gt MassHunter Workstation gt Data Acquisition To stop MassHunter Engines 1 Click Programs gt Agilent gt MassHunter WorkStation gt Launcher 2 Click Stop to stop the engines All engines will become red To start the engines click Start To stop MassHunter processes in case of unrecoverable errors When you stop MassHunter processes loss of data can occur e Click Programs gt Agilent gt MassHunter Workstation gt Acq Tools gt Remove MassHunter Processes To view the log book e Double click the Acq System Logbook icon in the Agilent MassHunter program group or click Start gt Programs gt Agilent gt MassHunter Workstation gt Acq System Logbook You can also view the logbook from within the
42. chem agilent com en US Support Downloads firmware Pages LC aspx and check that you have the most recent firmware for your instrument If you need to update your LC firmware follow the instructions on the web site Make sure you use the latest firmware update tool as instructed on the web site Check that the firmware for the G1369A Agilent LAN Interface card is the latest version available a Start a Command Prompt in Windows b Type telnet 192 168 254 11 in the Command Prompt window and press Enter c Type in the Telnet window The LAN Interface card displays its current configuration information The LAN Interface card requires 1 10 firmware or higher If an upgrade is needed please contact your local Agilent Sales Office Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration 2 Step 14 Finish the installation 1 Create a system recovery disk of your installed system Follow the instructions for the Backup Solution Bundle G1030 64002 included in the Agilent 6400 Series Triple Quad LC MS System bundle 2 Install or enable any other program that you will use with your MassHunter workstation such as MassHunter Optimizer or Compliance Refer the installation guide or quick start guide for these programs 3 Make sure that the trust settings in Excel are set for all MassHunter users including the guest account that is set up for compliance See step 6 of Step 7 Ins
43. click the Cell tab in the Tune Context and check that the high vacuum gauge reading is within range If not see To reset the Collision Cell gas flow rate on page 77 2 Start the Data Acquisition program change the Context to Tune and start an Autotune After the autotune has completed you may need to wait up to 30 minutes to allow for the calibrant solution to be pumped out of the Triple Quad This minimizes any background signal attributable to the calibrant In addition you can sonicate the nebulizer in a small graduated cylinder filled with acetonitrile for 10 minutes Parameters in the method can be modified by your Agilent representative for optimal response 3 Change the Context to Acquisition click File gt Load and then load the method 6460 ESI_AJT Pos MRM Checkout m The checkout method includes the following acquisition parameters 2 uL injection isocratic from channel A and B 70 30 at 0 4 mL minute 1 minutes run time 325 C Drying gas temperature 10 L minute Drying gas flow can be optimized typical 10 to 13 L min 20 psi nebulizer pressure can be optimized typical 20 to 35 psi 400 C Sheath gas temperature 12 L minute Sheath gas flow 4000 V capillary voltage 500 V nozzle voltage 41 V collision cell energy can be optimized typical between 37 to 41 V Agilent 6400 Series Triple Quad LC MS System Installation Guide 67 3 68 Installation Completion and System Verification 150 V fragmentor
44. d of the cable to the 10 100 Base T network card in slot 1 of the PC The card should be labeled LC MS Do not connect to port 8 on the hub switch Connect a second Category 5 STP LAN cable from one of the open ports 1 to 7 on the hub to the Agilent LAN Card in the 1100 or 1200 pump or detector Connect a third Category 5 STP LAN cable from one of the open ports 1 to 8 on the hub to the LAN interface connection on the LAN MS control card in the Triple Quad instrument Install the printer See the installation documentation supplied with the printer Turn on the printer hub monitor and PC in that order The computer comes loaded with software and the appropriate drivers for the installed accessories The initial Administrator password is provided on a sticker on the PC 32 Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration Step 2 Check PC network card con Step 2 Check PC network card configuration 1 Click Start gt Control Panel gt Network Connections 2 From the list of network connections right click the network connection that corresponds to the instrument LAN connection then click Properties 3 Scroll down the list to Internet IP Protocol click it then click Properties _ Local Area Connection 2 Properties General Authentication Advanced This connection uses the following items 8 QoS Packet Scheduler Broadcom Advanced Server Pro
45. e indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met Agilent 6400 Series Triple Quad LC MS System In This Guide This guide contains information to install the Agilent 6400 Series Triple Quad LC MS System 1 instrument Installation This chapter describes the steps that are needed to install the Triple Quad instrument 2 Software Installation and Configuration This chapter describes the steps that are needed to install the MassHunter software 3 Installation Completion and System Verification In this chapter you finish the hardware setup 4 installation and Verification of Other Sources This chapter contains the steps needed to install and verify the operation of the APCI and APPI sources 5 Other Setup Tasks This chapter contains tasks that are related to setting up your Triple Quad instrument 6 Reference This chapter contains parameters for verifying performance Agilent 6400 Series Triple Quad LC MS System Installation Guide 3 Agilent 6400 Series Triple Quad LC MS System Content 1 Instrument Installation 7 Step 1 Unpack and set up the Triple Quadinstrument 8 Step 2 Prepare to install the
46. gram Driver Description Transmission Control Protocol Internet Protocol The default wide area network protocol that provides communication across diverse interconnected networks Show icon in notification area when connected Notify me when this connection has limited or no connectivity Figure 15 Local Area Connection Properties dialog box General tab Agilent 6400 Series Triple Quad LC MS System Installation Guide 2 33 2 Software Installation and Configuration Step 2 Check PC network card configuration 4 Make sure the Network Adapter is set to these settings IP address 192 168 254 1 Subnet mask 255 255 255 0 Internet Protocol TCP IP Properties General You can get IP settings assigned automatically if your network supports this capability Otherwise you need to ask your network administrator for the appropriate IP settings Obtain an IP address automatically IP address 192 168 254 1 Subnet mask 255 255 255 0 Default gateway Obtain DN Use the following DNS server addresses Preferred DNS server Alternate DNS server Figure 16 Internet Protocol TCP IP Properties dialog box 34 Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration 2 Step 3 Prepare for installation CAUTION 1 If this installation is an upgrade use the Add or Remove Programs command in Control Panel
47. gram is pre installed on the shipping PC when ordered together with the instrument as a bundle If the program is already installed continue at Step 7 Install and configure Microsoft Excel 2007 on page 47 If this is an upgrade from the same revision such as from B 03 01 to B 03 01 SP1 continue at Step 5 Install the Qualitative Analysis program on page 41 1 Check that no other program is running on your system including Windows Update 2 Put the Data Acquisition installation disk into the disk drive The welcome screen appears See Figure 17 hee Agilent Technologies Agilent 6400 Series Triple Quad LC MS System Installation Welcome Uninstall Software Install Software Update Firmware Install Manuals ERD Agilent 6400 Series Triple Quad LC MS System Installation Agilent Agilent 6400 Series Triple Quad LC MS System Installation 7 g Follow these steps to install the MassHunter Data Acquisition program for the 6400 6400 Series Triple Quad Series Triple Quad LC MS LC MS System Installation Privacy Statement Terms of Use Agilent Home Agilent 2000 2010 Figure 17 Installation Welcome Screen 3 If this is an upgrade installation click Uninstall MassHunter Data Acquisition Software then follow the instructions on the screen to remove the current program Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration 2 4 Click Install MassHunter
48. guration 2 Step 6 Install the Quantitative Analysis program tl Library Editor Lae MS Quantitati TOF Quantitati Z Unknowns Analysis Figure 21 Desktop with Icons 3 If you want to install the Quantitative Compliance program a Open the folder SetupCompliance on the Quantitative Analysis installation disk b Double click setup exe 4 The compliance program is installed 5 From within a Windows file manager window check the installation disk for any folder that includes the word Service Packs Agilent 6400 Series Triple Quad LC MS System Installation Guide 45 2 Software Installation and Configuration 6 If one or more service pack files or folders exist on the installation disk then for each service pack file a Double click the name of the most recent service pack to run the installation program You may need to open folders inside the service pack folder to find the installation file The name of service pack installation files typically end in SP x exe b Follow the instructions to install the service pack 46 Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration 2 Step 7 Install and configure Microsoft Excel 2007 If you are doing an upgrade installation continue at Step 8 Install Service Packs for Data Acquisition on page 51 For new installations do this step even if Excel is already installed on your system Agilent Mass
49. hange button and select the path used to store data files click the OK button then click Next This data file location is typically the path used by the MassHunter Data Acquisition Workstation to store acquired data Agilent 6400 Series Triple Quad LC MS System Installation Guide 43 2 Software Installation and Configuration Step 6 Install the Quantitative Analysis program i When the Choose Desktop Icons window appears mark the check boxes for the instruments that apply Clear the check boxes for the instruments that do not apply Click Next Figure 20 ie Agilent MassHunter Workstation Quantitative Analysis InstallShield x Choose Desktop Icons Please choose icons to appear on your desktop Desktop icons appear for different presentations of Quantitative Analysis which are formatted to match the instrument characteristics QQQ triple quadrupole MS single quadrupole Q TOF quadrupole time of flight TOF time of flight Figure 20 Choose desktop icons window j When the Ready to Install the Program window appears click Install to begin When the installation begins you will see the Installing Agilent MassHunter Workstation Quantitative Analysis window k When the InstallShield Wizard Completed message is displayed click Finish Confirm that the icons you selected appear on your desktop Figure 21 Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Confi
50. heckout 460 BackgroundScan001 d Blank 1 2 _ AirBlank Vial 1 D MassHunter Data Checkout 6460 AirBlank001 d Blank 1 3 Solvent Blank Vial 2 D MassHunter Data Checkout 6460 SolventBlank001 d Blank al 4 Solvent Blank Vial 2 D MassHunter Data Checkout 6460 SolventBlank002 d Blank 1 5 Solvent Blank Viel 2_ D MassHunter Data Checkout 6460 SolventBlank003 d_ __ Blank 1 6 Solvent Blank Vial 2 D MassHunter Data Checkout 6460 SolventBlank004 d Blank 1 d Solvent Blank Vial 2 D MassHunter Data Checkout 6460 SolventBlank005 d Blank 1 8 Reserpine 500 fg Vial 3 D MassHunter Data Checkout 6460 ReserpineCheckout001 Sample 1 9 Reserpine 500 fg Vial 3 D MassHunter Data Checkout 6460 ReserpineCheckout002 Sample 1 10 gt v Reserpine 500 fg _ Vial3 D MassHunter Data Checkout 6460 ReserpineCheckout003 Sample_ iL 11 __ v Reserpine 500 fg Vial 3 D MassHunter Data Checkout 6460 ReserpineCheckout004 Sample 1 12 v Reserpine 500 fg Vial 3 D MassHunter Data Checkout 6460 ReserpineCheckout005 Sample 1 Worklist For Help press F1 6460 ESI_ AJT Pos MRM Checkout m D MassHunter 6460 ESI_AJT Pos MRM Checkout wkl is running Disk free space 189 65 GB CAP NUM SCRL Figure 31 Reserpine checkout worklist 1 Review the worklist to be sure that the method and data paths are correct and that the data file names given in the worklist are unique and have not already been acquired 2 Run the worklist 3 When the worklist is finished c
51. ibrant check box then acquire the data Verify that the masses in the MS1 and MS2 tabs are correct for the loaded source before you manually tune the peak Verify that you have sufficient abundance for the tune peaks that the tune peaks have peak widths of 0 7 0 05 amu and that mass assignments are within 0 15 amu of the target mass assignments 2 Save the tune file Agilent 6400 Series Triple Quad LC MS System Installation Guide 87 4 Installation and Verification of Other Sources HPLC Chip MS Cube Interface 88 Follow the steps in this section to do verification with the HPLC Chip MS Cube Interface Step 1 Prepare performance verification sample 1 Prepare the HSA peptides standard p n G2455 85001 by adding 500 uL of 15 85 acetonitrile water with 0 1 formic acid to the HSA peptide standard 500 pmol vial Mix well on a vortex mixer to completely dissolve the standard The resulting stock solution is 1 pmol uL and contains 7 peptides Only 1 peptide will be used for the quantitation checkout Create each of the dilutions listed in Table 6 a In a conical bottom polypropylene autosampler vial put the volume of standard in Table 6 b Add the volume of 15 85 acetonitrile water with 0 1 formic acid solvent in Table 6 The conical bottom polypropylene autosampler vials p n 5188 2788 and top with the appropriate caps p n 5182 0541 are included in the shipment Step E is optional and is used for collec
52. ick the D MassHunter folder and click Paste 4 In Windows Explorer right click the D MassHunter Support folder and click Properties Support Properties 2 x General Sharing Security Customize foal Suppor Type File Folder Location D MassHunter Size 58 1 MB 60 992 589 bytes Size on disk 58 4 MB 61 329 408 bytes Contains 159 Files 17 Folders Created Today August 29 2008 8 11 39 PM Advanced Attributes Figure 29 Support Properties dialog box 5 Clear the Read only check box and then click Apply The Confirm Attribute Changes dialog box will be displayed 56 Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration 2 Step 12 Copy the support folder to the computer Confirm Attribute Changes You have chosen to make the Following attribute changes unset read only Do you want to apply this change to this folder only or do you want to apply it to all subfolders and files as well O Apply changes to this folder only Figure 30 Confirm Attribute Changes dialog box 6 Click Apply changes to this folder subfolders and files then click OK Agilent 6400 Series Triple Quad LC MS System Installation Guide 57 2 58 Software Installation and Configuration Step 13 Confirm the LC firmware revision Start the Data Acquisition program Click File gt Print gt Instrument Configuration Go to http www
53. indow type the following and press Enter gt msepeek 1605 b Close the DOS window gt bye Agilent 6400 Series Triple Quad LC MS System Installation Guide 79 3 Installation Completion and System Verification To reset the Collision Cell gas flow rate 80 Agilent 6400 Series Triple Quad LC MS System Installation Guide Agilent 6400 Series Triple Quad LC MS System Installation Guide ee 4 Installation and Verification of Other e Sources G1947B APCI Source 82 To install the G1947B APCI source 82 To verify the operation of the G1947B APCl source 83 G1978B Multimode Source 84 To install the G1978B multimode source 84 To verify the operation of the G1978B multimode source 85 G1971B APPI Source 86 To install the G1971B APPI source 86 To verify the operation of the G1971B APPI source 87 HPLC Chip MS Cube Interface 88 Step 1 Prepare performance verification sample 88 Step 2 Prepare the HPLC Chip MS 90 Step 3 Analyze the results 93 This chapter contains the steps needed to install and verify the operation of the APCI multimode and APPI sources If the APCI multimode APPI or HPLC Chip MS source was purchased with the Triple Quad instrument you will need to install the source and verify its operation You can complete only a check tune with the different source types G1948B and G1978B Make sure you use the correct tune calibrant ot Agilent Technologies 81 4 Installation and Verification of Other Sources
54. ks 4 If one or more service pack files or folders exist on the installation disk then for each service pack file Double click the name of the most recent service pack to run the installation program Agilent 6400 Series Triple Quad LC MS System Installation Guide CAUTION Software Installation and Configuration 2 You may need to open folders inside the service pack folder to find the installation file The name of service pack installation files typically end in SP x exe b Follow the instructions to install the service pack If you plan to use a PCL6 printer download the print driver patch from http support microsoft com kb 935843 This patch is needed to correctly print reports in A4 format to HP PCL6 printers Set the Microsoft Image Writer as the default printer a Click Start gt Control Panel b Double click Printers and Faxes c Right click Microsoft Office Document Image Printer and click Set as Default Printer The Microsoft Image Writer formats the Excel workbooks three to four times faster for networked printers because the formatting page set up information is stored on the local computer The report is processed by the Microsoft Image Writer but it is actually printed on the printer that is selected in the MassHunter program If you do not set up a default printer such as the Microsoft Office Document Image Printer reporting can fail 7 Install the Save as PDF add in a On the Qualitative Ana
55. l A Prime the channel and then flush for 30 minutes at 1 mL minute with the 100 isopropyl alcohol to remove the flushing solvent 9 Put 250 mL of 100 methanol in a clean solvent bottle connect it to channel A and prime the channel 10 Install the 2 1 x 50 mm x 1 8 micron SB C18 Rapid Resolution column supplied with the instrument Flush the column out with 100 methanol for 1 hour at 1 mL minute minute 11 Use the high purity solvents that are shipped with the system Acetonitrile p n G2453 85050 Water p n 8500 2236 5 M formic acid p n G24538 85060 Add 1 mL of the 5 M formic acid solution to 1 liter of water for a final concentration of 5 mM 12 Pour 1 liter of acetonitrile in another clean solvent bottle 13 Connect the 1 liter of acetonitrile to channel A and 1 liter of water with 5 mM formic acid to channel B with 70 30 ratio and flush the system for 1 hour at 1 mL minute Make sure to flush through the Rapid Resolution checkout column Make sure that the solvent stream is going into the Triple Quad MS spray chamber Agilent 6400 Series Triple Quad LC MS System Installation Guide 63 3 Installation Completion and System Verification Step 2 Prepare the performance evaluation samples In this step you dilute the supplied performance evaluation samples to the concentrations needed for the Triple Quad system checkout Use the diluted samples within a day of dilution Refrigerate the intermediate first dilution in
56. lation Step 1 Unpack and set up the Triple Quad instrument Do not open the shipping containers until an Agilent representative is present to verify the contents of each carton Warranty claims for missing items are honored only if an Agilent representative is on site to verify the contents of each shipping container as it is unpacked Checkout and tuning samples are shipped separately to comply with safety regulations and to ensure prompt delivery Make sure that these samples have been stored correctly as indicated in the instructions in the box oa A Ww N Carefully examine all containers for external signs of damage If damage is discovered immediately contact the carrier and the Agilent Service Management Retain shipping containers and material until contents are checked for completeness and instrument performance is verified Check off each item on the packing list and verify the serial numbers Record the serial numbers in the installation documentation Report any discrepancies to Agilent Technologies Check that site preparation is complete which includes power and gas supplies and chemical supplies needed for installation and performance verification Check the line voltage Verify the proper line voltage and outlet ratings according to the instructions given in the Site Preparation Guide Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 Step 2 Prepare to install the syste
57. lation and Configuration Step 9 Install Quantitative Analysis Reporting Do this step only if you need to upgrade Quantitive Analysis Reporting 1 Check that no other program is running on your system Installation of the Quantitative Analysis Reporting program will fail if Windows Update is running even in the background 2 Install the Quantitative Analysis Reporting program a b Put the Quantitative Analysis installation disk into the disk drive If you need only to install the service pack for Quantitative Reports continue at step 3 From the root directory of the installation disk double click setup_quantreport exe In the Welcome to the InstallShield Wizard window click Next to continue with the installation In the License Agreement window click I accept the terms of the license agreement then click Next If needed in the Destination Folder window click Change to change the default destination location for the report templates The default is D MassHunter Report Templates Quant Click Next to continue When the Ready to Install the Program window appears click Install to begin When the installation begins you will see the Installing Agilent MassHunter Workstation Quantitative Analysis Reporting window When the InstallShield Wizard Completed message is displayed click Finish 3 From within a Windows file manager window check the installation disk for any folder that includes the word Service Pac
58. ly regulator at 10 to 30 psi 70 to 210 kPa Agilent 6400 Series Triple Quad LC MS System Installation Guide 21 1 Instrument Installation CAUTION Do not attach the Collision Cell gas fitting to the nitrogen supply regulator at 80 to 100 psi 550 to 690 kPa The Collision Cell Gas Flow Module should not receive a supply greater than 30 psi 210 kPa The Collision Cell can use the same nitrogen supply as the regular supply i e for drying gas nebulizer CDS pressure if it is the required purity 99 999 but it must be down regulated to 30 psi 210 kPa 11 Connect the remote start cable to its connector on the back left side of the Triple Quad See Figure 12 12 Connect the rough pump power cord into the rough pump connector on the back left side of the Triple Quad see Figure 12 13 Verify the front power switch is in the OFF position 14 Plug the Triple Quad power cord into the Triple Quad power connector on the Triple Quad and the other end of the Triple Quad power cord into the wall outlet See Figure 12 22 Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 Step 5 Install the source Step 5 Install the source You can use an electrospray the multimode source or the HPLC Chip for 6410 check out ESI with Agilent Jet Stream Technology 6460 only Before you begin check that you have these parts e Electrospray with Agilent Jet Stream Technology e ESI L Low Concentration
59. lysis or Quantitative Analysis installation disk open the folder Supplemental tools Excel2007 b Double click SaveAsPDFandXPS exe c Follow the instructions to install the add in This plug in lets you save and send reports in PDF format If you remove a MassHunter program and then install it again you may also need to re install the PDF plug in Agilent 6400 Series Triple Quad LC MS System Installation Guide 53 2 54 Figure 27 Software Installation and Configuration Step 10 Check Excel Add Ins for MassHunter 1 Close all MassHunter Workstation programs 2 Click Start gt All Programs gt Agilent gt MassHunter Workstation gt Acq Tools gt Remove MassHunter Processes to make sure that all MassHunter processes are stopped 3 Start Excel 2007 If you are warned that Excel is unable to find a MassHunter Add In click OK 4 Open the Add Ins dialog box a Click the Microsoft Office button then click Excel Options b From the navigation pane click Add Ins c Click the Go button next to the Manage drop down list 5 Verify that check boxes are marked for all add ins that begin with Masshunter then click OK See Figure 27 Add Ins Add Ins available Analysis ToolPak OK _ Analysis ToolPak VBA L Conditional Sum Wizard z Euro Currency Tools Internet Assistant VBA Lookup Wizard Browse 4 Masshunter Addin Loader z V Masshunter Reporting Acq Automation Masshunter Reporting Qual
60. m Step 2 Prepare to install the system Before you begin check that you have these parts e Utility knife and needle nose pliers 1 Unpack and set up the Triple Quad instrument a Cut the tie wraps around the package See Figure 2 Figure 2 Triple Quad instrument shipping container b Lift the boxes off the top of the instrument See Figure 3 Agilent 6400 Series Triple Quad LC MS System Installation Guide 9 1 Instrument Installation Step 2 Prepare to install the system Figure 3 Instrument unboxed on shipping pallet 6460 pictured c Remove the front top right back left and lower front left cosmetic covers so that you can remove the shipping brackets You do not need to remove the upper front left cover To remove the top cover remove the two screws at the back side of the top cover that secure the back of the top cover to the instrument chassis see Figure 4 These screws are only needed when shipping the instrument You do not need to reinstall the screws when you reinstall the top cover 10 Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 Step 2 Prepare to install the system gt P Figure 4 Remove shipping screws from back of top panel The screws to remove are circled in this picture d Remove the shipping brackets a total of 7 You will need to use a 13 mm wrench Figure 5 Triple Quad on shipping pallet with covers removed 6460 pictured Agilent 640
61. ment Configuration program Programs gt Agilent gt MassHunter Workstation gt Acq Tools gt Instrument Configuration 5 Under Mass Spectrometer click the Triple Quad instrument Leave all check boxes cleared EP Instrument Configuration Instrument Name Instrument 1 Mass Spectrometer Agilent 6400 Series Triple Quadrupole 192 168 254 12 C Agilent 1100 1200 System Access C Agilent 35900E A D Converter Figure 39 Instrument Configuration 6 Click Configure Agilent 6400 Series Triple Quad LC MS System Installation Guide 103 5 Other Setup Tasks 7 Start the MassHunter Workstation Acquisition program and confirm that only the MS instrument is available 8 Close the Acquisition program 9 Stop MassHunter processes 10 Open Instrument Configuration again 11 Click the Triple Quad instrument and mark the Agilent 1100 1200 1290 System Access check box Agilent BOUU Senes Wuadrupole Lime of Fight 192 158 25412 O Agilent 6200 Series Time of Flight Agilent 1100 1200 1290 System Access 192 168 254 11 C Agilent 35900E A D Converter Figure 40 Instrument Configuration dialog box 12 Click Configure The registry has now been cleared and configured for any new LC module s 104 Agilent 6400 Series Triple Quad LC MS System Installation Guide Agilent 6400 Series Triple Quad LC MS System n Installation Guide a 6460 6430 Reserpine Checkout Method Acquisition Parameters 106 5 ee
62. ons vary widely from site to site get assistance from an IT representative when you connect to the site network Configuration by an Agilent Customer Engineer is on a best effort basis only Agilent 6400 Series Triple Quad LC MS System Installation Guide 99 To confirm configuration of NICs In this step you confirm that the correct LAN configuration exists 1 Click the Start button and click Run 2 Type CMD for the command prompt A command window appears 3 At the command prompt type ipconfig all See Figure 38 for an example screen oft Windows 2666 Version 5 66 2195 lt C Copyright 1985 2000 Microsoft Corp iC gt ipconfig all Windows 2000 IP Configuration Host Name Primary DNS Suffix Node Type IP_Roweiiig Enabled No WINS Proxy Enabled No DNS Suffix Search List ses agilent com picard_ps AYvP IS Ethernet adapter Local Area Connection 2 Connection specific DNS Suffix Description I Intel R gt PRO 1066 MI Desktop Adapter Physical Address 6 7 E 11 98 41 DHCP Enabled a No TP AGAPS S E7 0 2 Eaa eh fe ep a 192 168 254 1 Subnet Mask s s o e a aoa 255 255 255 8 Default Gateway DNS Servers Eth rast adapter Local Area Connection ConnectYewesmecific DNS Suffix scs agilent com Description eeDrvaucom Netktreme Gigabit Ethernet for hp Physical Address 66 B CD B3 AF B4 DHCP En
63. ove the electrospray source and install the multimode source onto the spray chamber mounts Connect the multimode and corona needle cables to the respective connectors on the left side panel of the Triple Quad instrument Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation and Verification of Other Sources 4 To verify the operation of the G1978B multimode source 1 Check the tuning of the Multimode source a b In the Context list click Tune in the Data Acquisition program Load the autotune file that was generated with the electrospray or multimode source In the Manual Tune tab mark the Calibrant check box then acquire the data Verify that the masses in the MS1 and MS2 tabs are correct for the loaded source before you manually tune the peaks Verify that you have sufficient abundance for the tune peaks that the tune peaks have peak widths of 0 7 0 05 amu and that mass assignments are within 0 15 amu of the target mass assignments 2 Save the tune file Agilent 6400 Series Triple Quad LC MS System Installation Guide 85 4 Installation and Verification of Other Sources G1971B APPI Source 86 To install the G1971B APPI source 1 Install the APPI USB to Serial Converter Cable p n 8121 1013 to the one of the available USB ports on the SmartCard on the left side of the Triple Quad instrument Pour the Electrospray calibrant back into its original bottle or another suitable cont
64. purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend U S Government Restricted Rights Soft ware and technical data rights granted to the federal government include only those rights customarily provided to end user cus tomers Agilent provides this customary commercial license in Software and techni cal data pursuant to FAR 12 211 Technical Data and 12 212 Computer Software and for the Department of Defense DFARS 252 227 7015 Technical Data Commercial Items and DFARS 227 7202 3 Rights in Commercial Computer Software or Com puter Software Documentation Safety Notices CAUTION A CAUTION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until th
65. re Microsoft Excel 2007 Microsoft Office Excel Office Shared Features Office Tools Click Continue and continue the installation as instructed Microsoft Office Professional 2007 Installation Options Customize how Microsoft Office programs run Microsoft Office Microsoft Office Outlook Microsoft Office PowerPoint Microsoft Office Publisher Microsoft Office Visio Viewer Microsoft Office Word Office Shared Features Office Tools Figure 22 Microsoft Office installation options For Excel 2007 you will only see Micro soft Office Excel Office Shared Features and Office Tools 5 When you are prompted to get updates click Go to Office Online Follow the instructions to update Excel 2007 or Office 2007 Make sure Service Pack 2 is installed for optimal performance of MassHunter reporting Cin cia Microsoft Office Professional 2007 has been successfully installed Visit Microsoft Office Online to get free product updates help and online services Qose Figure 23 Click the Go to Office Online button to update Excel 48 Agilent 6400 Series Triple Quad LC MS System Installation Guide Software Installation and Configuration Step 7 Install and configure Microsoft Excel 2007 6 Check that Excel security for MassHunter Workstation add ins is configured a Open Excel N b Click the Microsoft Office Button Ba c Click Excel Options See Figure 24 CET Ap
66. s and a pair of needle nose pliers to secure the oil return tubing Rough pump hose Exhaust hose Oil mist filter Ballast valve Oil return line Oil level sight glass Oil drain plug Oil drip pan Figure 7 Example rough pump Note that your pump may look slightly different 7 Check that the ballast valve is closed Closing the ballast valve prevents additional air from being introduced to the pump This allows the rough pump to reach a lower operating pressure CAUTION The ballast valve must be opened fully counterclockwise periodically to allow oil trapped in the mist filter to return to the pump If the oil is not returned to the pump the pump will eventually run dry and be damaged 14 Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation 1 8 Find the Low Profile Exhaust Adaptor p n G1960 20003 and the O ring p n 0905 1592 Put the O ring on the exhaust adaptor 9 Locate one KF 25 swing clamp p n 0100 1398 open the swing clamp and use it to clamp the exhaust adaptor onto the oil mist filter Connect the 1 2 inch I D Tygon exhaust tubing to the exhaust adaptor Agilent 6400 Series Triple Quad LC MS System Installation Guide 15 1 Instrument Installation Step 4 Connect the Triple Quad instrument CAUTION 1 Connect the rough hose p n G1969 20123 that is supplied in the ship kit to the KF 25 rough pump hose connection on the back left of the instrumen
67. set to Unit Agilent 6400 Series Triple Quad LC MS System Installation Guide 71 3 72 Installation Completion and System Verification Edit the method to ensure that for channel A the 75 25 methanol water solution with 5mM ammonium formate is selected as the LC solvent Click File gt Load and then load the method MMI ES Pos MS2 Scan m Edit the method to ensure that for channel A 75 25 methanol water solution with 5mM ammonium formate is selected as the LC solvent Put the vials into the LC autosampler Position 1 An empty uncapped vial Position 2 A vial containing the solvent used for dilution this is the solvent blank Position 3 A vial containing the reserpine sample 500 fg uL From the File menu click Load and then load the worklist MMI ES Pos Reserpine Checkout wkl The worklist is set up to do one injection of the solvent blank using the ESI Pos MS2 Scan m method in order to collect background ion data then using the ESI Pos MRM Reserpine Checkout m method for the remaining runs one injection of the empty vial five injections of the solvent blank and five injections of the reserpine sample Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation Completion and System Verification 3 iple Que z Sample Name Sample Position Data File Sample Type Level Name Inj Yol yl Comment 1 BackgroundScan vi __ D MassHunter Date C
68. t Connect the rough pump hose to the KF 25 inlet adapter on the rough pump Use the supplied hook loop fastener tape to secure the drain bottle into a secondary containment tub Connect the spray chamber drain tube to the 1 inch fitting on the drain bottle The drain tube can also be routed out the front of the Triple Quad if needed Attach the calibrant delivery system CDS inlet module waste tubing from the solvent selection valve on the Triple Quad to the 4 inch fitting on the drain bottle Connect a length of the 12 inch Tygon tubing to the 12 inch fitting on the drain bottle then connect the other end of the tubing to a vent connection that is separate from the vent used for the rough pump The drain bottle vent must be located away from the rough pump vent to prevent the rough pump exhaust from contaminating the Triple Quad spray chamber 16 Agilent 6400 Series Triple Quad LC MS System Installation Guide Instrument Installation Step 4 Connect the Triple Quad instrument Spray chamber drain tube Drain bottle vent CDS inlet and LC waste fitting Waste drain bottle Secondary containment customer supplied Figure 8 Drain bottle Agilent 6400 Series Triple Quad LC MS System Installation Guide 1 Instrument Installation Step 4 Connect the Triple Quad instrument 7 Install the gas filter For 6410 6430 18 Connect an appropriate length of the 1 4 inch teflon gas tubing G1946
69. t secure the pump to the wooden pallet 3 Set the pump into the pump oil drip pan The pan is used to catch any small amounts of oil that may seep out of the pump The pan can also contain all of the oil in the pump in case of a catastrophic seal failure 4 Fill the rough pump with Inland 45 fluid The rough pump is drained of vacuum pump oil prior to shipment Remove the oil fill cap and use the funnel to add Inland 45 pump fluid until the level in the site glass is approximately 2 3 full 5 Install the oil mist filter Remove the plugged outlet connector and install the new KF 25 fitting that comes with the pump Do not use the outlet nozzle as described in the Edwards manual and on the warning tag Install the mist filter on the outlet port KF 25 fitting See figure on the next page Make sure the mist filter drain plug is facing the rough pump ballast valve toward the motor end of the pump 6 Install the oil return line a Remove the drain plug from the mist filter and install the drain adapter b Install the brass restrictor into the black oil return tubing c Connect the oil return tubing between the rough pump ballast port fitting and the oil mist filter drain adapter Make sure the end of the tubing with the brass restrictor is installed at the ballast port fitting Agilent 6400 Series Triple Quad LC MS System Installation Guide 13 1 Instrument Installation Step 3 Set up the rough pump d Use the plastic hose clip
70. t destination location The default is C Program Files Agilent MassHunter Workstation Qual In the Choose location for customer data files screen check that the default of D MassHunter is displayed in the text box then click Next See Figure 19 Agilent 6400 Series Triple Quad LC MS System Installation Guide 41 2 42 Software Installation and Configuration Agilent MassHunter Workstation Installation Choose location for customer data files Agilent Technologies Select a folder for customer specific files like data files and methods You can type a new folder or select a new folder with the Browse button Click Next when the folder has been selected ID MassHunter g h Browse lt Back Cancel Figure 19 Location for data files In the Start Copying Files screen review the settings then click Next If you get a message that indicates that the screen resolution is not set to the required 1280 x 1024 resolution click OK The best resolution for MassHunter is either 1600 x 1200 for 4 x 3 aspect ratio or 1920 x 1080 for 16 x 9 If you see a Notepad window that prompts you to install other options close the Notepad window 3 When the InstallShield Wizard Complete message is displayed click Finish 4 From within a Windows file manager window check the installation disk for any folder that includes the word Service Packs 5 If one or more service pack files or folders exist on the installation
71. tall and configure Microsoft Excel 2007 4 Install the programs that are part of the Agilent Software Backup Solution and do a system back up of the computer Refer to the H1172A Backup Solution Installation and User Guide and the Agilent Software Backup Solution Hardware and Software Installation Checklist for details Agilent 6400 Series Triple Quad LC MS System Installation Guide 59 2 Software Installation and Configuration Step 14 Finish the installation 60 Agilent 6400 Series Triple Quad LC MS System Installation Guide Agilent 6400 Series Triple Quad LC MS System Installation Guide c00 ee 3 Installation Completion and System gt Verification Step 1 Condition the 1100 1200 LC 62 Step 2 Prepare the performance evaluation samples 64 Step 3 Review methods and run worklist 66 Step 4 Verify the Triple Quad sensitivity 73 To reset the Collision Cell gas flow rate 77 This chapter contains the steps needed to complete the hardware setup and verify the performance specifications of the installed system ee Agilent Technologies 61 3 62 Installation Completion and System Verification Step 1 Condition the 1100 1200 LC Before you begin be sure that you have these parts FPF WH High purity HPLC grade isopropanol supplied by customer Premixed Flushing Solvent p n G1969 85026 High Purity Water p n 8500 2236 High Purity Methanol p n 8500 1867 High Purity Acetonitrile p n
72. termine the optimal flow rate DAC steps to achieve the correct high vacuum gauge reading Enter this value into a Data Acquisition configuration file 1 Determine the optimal Flow Rate a Open a DOS window click Start gt Run type cmd and click OK b Type telnet 192 168 254 12 5123 to open a back door into the Triple Quad s MS Interface Card firmware c Press Enter d Type msepeek 1605 at the gt prompt to view the current value in DAC Steps for the Flow Rate You should get a response like this 61577 0 backdoor command msepeek 1605 12 61577 0 MSE_READ address 1605 value 520 520 is the factory default value e Find the high vacuum gauge reading in the Cell tab of the Manual Tune program f Use the syntax below to adjust the value for register 1605 until the high vacuum gauge reading in the Manual Tune program is in the range of 1 9 to 2 3 x 10e gt msepoke 1605 value For example to set the 1605 register value to 550 type the following and press Enter gt msepoke 1605 550 You may need to try numbers that are above and below the default value g Write this number down Agilent 6400 Series Triple Quad LC MS System Installation Guide 77 3 Installation Completion and System Verification 2 Edit the acquisition parameter file so that the newly determined value will be applied whenever Data Acquisition starts up and will be used for tuning and data acquisition d e Open
73. the D MassHunter instr default folder then open the appropriate xml file with Notepad 6460 G6460AHWParams xml 6430 G6430AHWParams xml 6410 G6410BHWParams xml Locate the set point for the Collision Cell gas flow between the lt flowrate gt and lt flowrate gt tags In this example the set point for the flow rate is 520 lt cellFlow gt lt flowA gt 1 lt f lowA gt lt flowB gt 0 lt flowB gt lt flowrate gt 520 lt flowrate gt lt cellFlow gt Change the flow rate set point to the value that you wrote down as the optimal flow rate In this example the value is changed to 550 lt cellFlow gt lt flowA gt 1 lt flowA gt lt flowB gt 0 lt flowB gt lt flowrate gt 550 lt flowrate gt lt cellFlow gt Click File gt Save to save the file Click File gt Exit to close the file 3 Restart the Data Acquisition program a b 78 Close the Data Acquisition program Close all MassHunter engines from the MassHunter Acquisition Engine launcher Click the Acq System Launcher icon Restart the MassHunter engines Start the Data Acquisition program You should now read the high vacuum value which you targeted for and can proceed with the installation as described Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation Completion and System Verification To reset the Collision Cell gas flow rate 4 Verify the Collision Cell gas flow a In the DOS w
74. ting information Table6 Dilutions for HSA peptides standard Sample Volume of Standard Volume of Solvent Final Concentration A 10 uL of 1 pmol pL solution 90 pL 100 fmol pL B 10 pL of A 90 pL 10 fmol pL C 10 pL of B 90 pL 1 fmol pL D 10 pL of C 90 pL 100 amol pL E 10 pL of D 90 pL 10 amol uL Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation and Verification of Other Sources 4 4 Create a blank vial of 15 85 acetonitrile water with 0 1 formic acid Use the same conical vials and caps used for the HSA samples 5 Prepare a vial containing 60 40 acetonitrile water with 1 TFA to clean the injector before doing low level samples This solution works well for solubilizing hydrophobic peptides If a clean blank cannot be achieved after running several injections of this solution remove the needle seat with seat capillary and place it seat side down in a beaker with this solution then sonicate for 5 to 10 minutes Agilent 6400 Series Triple Quad LC MS System Installation Guide 89 4 Installation and Verification of Other Sources Step 2 Prepare the HPLC Chip MS e Copy the files from the Support folder to the Methods and Worklists folders according to Table 7 Table 7 Methods and worklists to copy Copy From To 6400 HPLC Chip HSA Checkout m D MassHunter Support Checkout 6400 Methods and D MassHunter Methods Worklist Pos HPLC Chip HSA Checkout 6400 HPLC CHip HSA Checko
75. to remove programs that you will be upgrading if they are installed on your system Do not remove programs for which you need only to install a service pack Excel 2003 Agilent MassHunter Workstation Qualitative Analysis Agilent MassHunter Workstation Quantitative Analysis Agilent MassHunter Workstation Quantitative Analysis Reporting Agilent MassHunter Workstation Optimizer Remove only the programs that you plan to upgrade at this time Do not remove the Agilent MassHunter Workstation Data Acquisition program at this time Optional Back up the data method and worklist files in the D MassHunter folder Use the back up method of your choice The uninstallation program keeps your data method and worklist files It also backs up your tuning and hardware setting files which are restored during installation Do not move remove or rename the D MassHunter folder or your tuning and hardware settings will not be saved Run Windows Update to make sure you have the latest critical updates and security fixes Make sure Windows Update is completed before you continue If you do not run Windows Update your system may be vulnerable to security problems Confirm that the LAN power management has not been reactivated by a Windows Update program Agilent 6400 Series Triple Quad LC MS System Installation Guide 35 2 36 Software Installation and Configuration Step 4 Install the Data Acquisition program The Data Acquisition pro
76. ts 2 volts 3 volts 4 volts 5 volts 6 volts volts The current setting is 8 volts Line Voltage is this is only for shipment press return to use current one or Input the item number to select line voltage For example in the United States the closest nominal voltage for the Triple Quad instrument is 208V so you would select option 3 The ESI with Agilent Jet Stream Technology uses 95 input voltage duty cycle to heat properly Improper settings can severely hamper its performance Agilent 6400 Series Triple Quad LC MS System Installation Guide 39 2 40 Software Installation and Configuration b When prompted confirm input voltage set in the previous step OK to proceed Y YES gt y Clearing old Retreiving 192 168 254 12 9QQDefaultParms 888 Nominal line lt Shipping 1986 volts volts volts A volts volts volts volts Is this correct y 1 Sending 192 168 254 12 home sc4 qqq f ileupdate Sending 192 168 254 12 qqq qqqServer Sending 192 168 254 12 qqq QQQ_DSP_FW ldr update the files and reboot qqqServer firmware voltage Default gt 3 208 volts current one or to select line voltage 3 set to 208 c Reboot the LC MS interface card or turn on and off the instrument when prompted Once the firmware update has completed you will be prompted to close the DOS window 9 Optional Click Install MassHunter Data Acquisition Manuals to copy
77. ure 44 Column tab Agilent 6400 Series Triple Quad LC MS System Installation Guide 107 6 Reference 6460 6430 Reserpine Checkout Method Acquisition Parameters Sample Properties hALS SL BinPump SL Column SL DAD SL MS r Tune file gt Stop time on Source Chromatagram Instrument Diagnostics atunes TUNE XML No limits Pump Scan segments om I Browse e C 20 Ud Precursor lt Product Collision Compound Name irer bn MS1 Res ea MS2Res Dwell Fragmentor Energy Polarity p lon source Time filtering l gt Reserpine 150 ESI v Agilent Jet Stream V Peak width 003 min r Time segments Start i Delta Delta i Scan Type Div Valve EMV EMV E Stored IE o MAM Toms 200 o m ae cycles s Bas ms eycle Figure 45 MS Q00 tab 6460 only Acquisition bs i Chromatogram Instrument Diagnostics m Source parameters Gas Temp Ba G 326 E Zy Paste Gas Flow 10 Imin 10 0 min Nebulizer 20 psi 200 psi Paste to AIl Sheath Gas Temp 400 C E Segments Sheath Gas Flow 12 Imin 120 Imin Positive Negative Capillary 4000 v 3500 v 2000 nA Nozzle Voltage fo v lo y Chamber Current 0 18 pA Figure 46 MS QQQ Source tab for ESI with Agilent Jet Stream Technology 6460 only 108 Agilent 6400 Series Triple Quad LC MS System Installation Guide Reference 6 6460 6430 Reserpine Checkout Method Acquisition Parameters
78. ut wkl D MassHunter Support Checkout 6460 Methods and D MassHunter Worklists Worklist Pos HPLC Chip HSA Checkout If you want to create your own method and worklist do these steps 1 Create a worklist in MassHunter Acquisition program to run the prepared samples See Figure 34 If you are time limited you can do a single injection of each but triplicate injections are preferred The 10 amol HSA level is optional Remember to condition a new chip with 500 fmol of HSA 3 times Then clean up with blanks and the solubilizing solution f Sample Name Sample Position Method Data File Sample Type Level Name Inj Vol pl Comment 1 blank P1 D1 43mm Smin hser mem 575 unitm Iblank r001 d Blank ji j 2 blank P1 D1 43mm Smin hse mrm 575 unit m blank 1002 d Blank 1 3 blank P1 D1 43mm Smin hse mrm 575 unitm jblank 1003 d Blank a 4 100 amol ul HSA PI F5 43mm Smin hse mem 575 unitm 100amol 001 d Calibration 1 1 5 100 amol ul HSA PI F5 43mm Smin hse mrm 575 unit n 100amol1002 d Calibration 1 j1 6 100 amol ul HSA P1 F5 43mm Smin hse mrm 575 unitm 100amol 003 d Calibration 1 1 Z 1 fmol ul HSA P1 F6 43mm 3min hsa mrm 575 unitm 1fmol r001 d Calibration 2 a j 8 1 fmol ul HSA P1 F6 43mm Imin hsa mrm 575 unitm 1fmol r002 d Calibration 2 1 g 1 fmol ul HSA P1 F6 43mm Smin hse mrm 575 unit m Timol 1003 d Calibration 2 10 10 fmol ul HSA P1 F 43mm Smin hse mrm 575 unit m 10fmol r001 d Calibration 3 a
79. ution set to Wide and MS2 Resolution set to Unit Agilent 6400 Series Triple Quad LC MS System Installation Guide 69 3 70 Installation Completion and System Verification Edit the method to ensure that for channels A and B 70 30 acetonitrile water with 5mM formic acid is selected as the LC solvent Click File gt Load and then load the method 6400 ESI Pos MS2 Scan m Edit the method to ensure that for channel A 70 30 acetonitrile water with 5mM formic acid is selected as the LC solvent Put the vials into the LC autosampler Position 1 An empty uncapped vial Position 2 A vial containing the solvent used for dilution this is the solvent blank Position 3 A vial containing the reserpine sample 500 fg uL From the File menu click Load and then load the worklist 6400 ESI Pos MRM Checkout wkl The worklist is set up to do one injection of the solvent blank using the 6400 ESI Pos MS2 Scan m method in order to collect background ion data then using the 6400 ESI Pos MRM Checkout m method for the remaining runs one injection of the empty vial five injections of the solvent blank and five injections of the reserpine sample Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation Completion and System Verification 3 For 6410 G1978B Multimode Interface Positive MRM Mode 1 Verify that the Collision Cell gas flow rate produces a high vacuum gauge reading in the range of 2 7 to 3 3 x 10e 5 torr
80. voltage can be optimized typical between 140 to 250 V 200 V Delta EMV can be optimized typical between 200 to 400 V MS1 Resolution set to Wide and MS2 Resolution set to Unit Edit the method to ensure that for channels A and B 70 30 acetonitrile water with 5mM formic acid are selected as the LC solvent Click File gt Load and then load the method 6460 ESI_AJT Pos MS2 Scan m Edit the method to ensure that for channels A and B 70 30 acetonitrile water with 5mM formic acid is selected as the LC solvent Put the vials into the LC autosampler Position 1 An empty uncapped vial Position 2 A vial containing the solvent used for dilution this is the solvent blank Position 3 A vial containing the reserpine sample 500 fg uL From the File menu click Load and then load the worklist 6460 ESL AJT Pos MRM Checkout wkl The worklist is set up to do one injection of the solvent blank using the 6460 ESI_AJT Pos MS2 Scan m method in order to collect background ion data then using the 6460 ESI_AJT Pos MRM Checkout m method for the remaining runs one injection of the empty vial five injections of the solvent blank and five injections of the reserpine sample Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation Completion and System Verification 3 For 6410 6430 6460 G1948B Electrospray Interface Positive MRM Mode 1 Verify that the Collision Cell gas flow rate produces a high vacuum gauge reading
81. y the methods and worklists according to your instrument model Table 3 Methods and worklists to copy If you have Copy From To 6460 ESI with 6460 ESI_AJT Pos M82 Scan m D MassHunter Support D MassHunter Methods Agilent Jet 6460 ESI_AJT Pos MRM Checkout m Checkout 6460 Methods and Stream Worklist Pos Technology 6460 ESI_AJT Pos MRM Checkout wk D MassHunter Support D MassHunter Worklists Checkout 6460 Methods and Worklist Pos 6410 6430 6400 ESI Pos MRM Reserpine Checkout m D MassHunter Support D MassHunter 6460 ESI 6400 ESI Pos MS2 Scan m Checkout 6400 Methods and Methods ESI Pos Reserpine Checkout wkl Worklist Pos ESI Pos MRM Checkout D MassHunter Support Checkout 6400 Methods and Worklist Pos ESI Pos MRM Checkout D MassHunter Worklists Depending on your instrument model go to For 6460 ESI with Agilent Jet Stream Technology Positive MRM Mode on page 67 For 6410 6430 6460 G1948B Electrospray Interface Positive MRM Mode on page 69 For 6410 G1978B Multimode Interface Positive MRM Mode on page 71 66 Agilent 6400 Series Triple Quad LC MS System Installation Guide Installation Completion and System Verification 3 For 6460 ESI with Agilent Jet Stream Technology Positive MRM Mode 1 Verify that the Collision Cell gas flow rate produces a high vacuum gauge reading in the range of 1 9 to 2 3 x 10e torr To view the high vacuum gauge reading
82. you to install other options close the Notepad window When the InstallShield Wizard Complete message is displayed click Finish 5 Click Upgrade MS Firmware and press Enter 7 When prompted type Y for yes to proceed with update ex D WWDemoCD B15 OQQFW_47 QqQUpdate exe D WWDemoCD B15 gt QQQFWl_47 QqQUpdate exe version 1 60 Enter instrument ip 192 168 254 12 192 168 254 12 communications check PASSED SmartcardIU detected MSD controller 192 168 254 12 will be updated f gt gt DO NOT DISRUPT POWER TO MSD DURING THE UPDATE lt lt t OK to proceed Y YES Agilent 6400 Series Triple Quad LC MS System Installation Guide Type the IP address of the Triple Quad instrument 192 168 254 12 CAUTIO Software Installation and Configuration 2 8 If the instrument is using an ESI with Agilent Jet Stream Technology a When prompted enter the country specific nominal input voltage Verify with a Digital Multi Meter DM using the AC Voltage measuring function uments and Settings Administrator My Documents QQQ_B2018 QQ0FW_ Enter instrument ip 192 168 254 12 192 168 254 12 communications check PASSED SmartcardIU detected MSD controller 192 168 254 12 will be updated tt gt gt DO NOT DISRUPT POWER TO MSD DURING THE UPDATE lt lt tt OK to proceed CY YES gt y Clearing old firmware Retreiving 192 168 254 12 QQQDefaultParms 908 Nominal line voltage O lt Shipping Default gt 198 vol

Download Pdf Manuals

Related Search

Related Contents

Keychain Barcode Scanner User Guide  Wave BitLocker Manager  Emerson CKD1612 CD Player User Manual  Ergonomic Lift Table Manual  König PCI WLAN  Hydricpure  Istruzioni per l`uso TOP 280 EUROTOP 280  V7 CAT 6 Cable FSTP Foiled Shielded Halogen Free Grey 10m  Oparation Manual  Manual de instrucciones terminal de mano V1.3  

Copyright © All rights reserved. Failed to retrieve file