EDP3 Plus Single Channel User Manual 2002
Contents
1. then the hidden modes in order to switch them on DILUTE TITRATE MANUAL GLP then the snp option sound When a hidden mode is displayed switch it on or off using the ARROW keys Then press RESET or a trigger to lock the mode state Pressing a trigger also starts a cycle in the selected mode Pc wn DILUTE mode switched on in MULTI mode mu using ARROW keys an Litt If all modes are switched on pressing the mode key repeatedly cycles through them as follows PIPET MULTI DILUTE TITRATE MANUAL GLP Operation in all modes is shown in the pages following Within each mode pressing and holding the mope key opens the OPTIONS Menu appropriate for that mode Switch the options on or off and set increasing or decreasing values for some options with the arrow keys To lock options in memory press RESET or a TRIGGER Pressing a TRIGGER starts the cycle as well Options Common to All Modes SND OPTION Sound on off volume level IN MULTIDISPENSE mode press and hold the move key to reach the options menu Then press the mope key repeatedly until snp is displayed Use either arrow key to turn this option on or off and set the volume level between 1 soff and 7 loud The on off state and volume level are set for all modes PERY MULTI Sed ant SND OPTION turned on with volume set to level 4 With snp switched on the following sounds are heard PIPET MODE End of pickup HIGH BEEP2. Table applies to EDP3 Plus in Pipet mode Advanced Modes and Options PIPET MODE Basic operation of pipet mode is described on pages 4 5 OPTIONS AVAILABLE amp MIX CC SEQ FP SPEED PIPET amp MIX OPTION Piston moves up and down while the trigger is pressed mixing the sample and another liquid in the tip In PIPET Mode press and hold the mope key to reach the options menu Then click the mope key until amp mix appears on the display The first time used the display will flash orr Use the arrow keys to turn on the option and set the volume to be mixed from 5 to 100 of nominal volume PIPET A MILE j PIPET amp MIX OPTION selected with 50 pL mix volume 5 nn LLL br Press RESET and set the aspiration volume with the arrow keys Then immerse the tip end into the sample and press either trigger to aspirate the set volume Pressing and holding either trigger dispenses the set volume then mixes the mix volume in the tip by moving the piston up and down while the trigger is pressed To stop mixing and dispense the tip contents release the trigger Alternately a single touch to the trig ger dispenses without any mixing occurring To turn off the mix option hold the mope key until amp mix is dis played then scroll the mix volume with the arrow keys to the min imum or maximum value Releasing and pressing the last ARRow key turns the option off SEQ OPTION IN PIPET MODE Volume S
3. VAC Volts Alternating Current VDC Volts Direct Current 26 Appendix A GLP Modes GLP mode is a special mode for viewing tracking and saving var ious usage log information e g the number of pipetting cycles or number of days in use eLp mode could also be used to track non service issues such as the number of days a particular pipette is used for a particular lab task and so on GLP mode is accessed though the mutt mode In murti mode press and hold the mope key to reach the options and hidden modes click mope until GLP is reached then switch on eLp mode with the ARROW key Press RESET and click mope until GLP is reached i GLP mode is on showing the Pres ent set of usage logs SiP Pr amp S a GLP mode shows various usage logs for EDP3 Plus The first level of GLP logs without options is reached by pressing Reset to cycle through these logs in turn as shown below i The cycle count since the last service entry D SEr Press RESET SE 165 i Total cycle count ALL of EDP3 Plus in thousands it J Press RESET ah i Number of days dAS since the last service entry das ie GLP Options OPTIONS AVAILABLE AL dAL Add GLP options are accessed from within the eLp mode by a long press of the mope key Cycle through each in turn by clicking Mope Cycle count alarm set to 10000 cycles ARROW keys to set in thousands note that as the num Bo ber gets into 10 000s the significant unit 1 7 Fn
4. End of dispense Low BEEP Return to home DOUBLE HIGH BEEP MULTIDISPENSE MODE End of pickup HIGH BEEP After each dispensed aliquot HIGH BEEP After last aliquot DOUBLE HIGH BEEP ONE EXTRA LOW BEEP Bottom of blowout Low BEEP Return to home DOUBLE HIGH BEEP DILUTE MODE After all pickups 1 to n 1 HIGH BEEP After last pickup DOUBLE HIGH BEEP ONE EXTRA LOW BEEP Bottom of blowout Low BEEP Return to home DOUBLE HIGH BEEP MANUAL MODE After full pickup HIGH BEEP At end of dispense HIGH BEEP Bottom of blowout Low BEEP Return to home DOUBLE HIGH BEEP TITRATE MODE After full pickup HIGH BEEP At end of dispense HIGH BEEP Bottom of blowout Low BEEP Return to home DOUBLE HIGH BEEP GENERAL ALL MODES Key press SHORT CLICK Low battery warning LouD warBLeE continuous alternating high low beep after every motor movement when there is a low battery condition even when snd is turned off Error LOUD WARBLE when any error condition is present even if SND is turned off CC OPTION Cycle counter Each pipetting cycle is counted In any mode except MULTIDISPENSE press and hold the mope key to reach the options menu Then press the mope key repeatedly until cc is displayed Use either arrow key to turn this option on or off The display shows incrementing pipetting cycles in the lower left up to 999 then rolls over to O You can reset the counter to O at any time by pressing and holding reset until t
5. FP volume to start again and the pown arrow scrolls through the fixed volume settings in descending order rolling over after the first FP volume Turn the FP option off by holding the mope key to open the options menu and pressing the mope key repeatedly until you reach FP ON Then press either arrow key Notes 1 The arrow keys select the next FPn in sequence NOT the next higher or lower volume 2 If the FP option is set on scrolling through the pipet mode options by holding then repeatedly pressing the move key also scrolls through all FP volumes even where no entry was made 11 MULTIDISPENSE MODE For basic operation mULTIDISPENSE Mode see pages 5 6 OPTIONS AVAILABLE AUTO SEQ SND SPEED MODE SWITCH ON DILUTE TITRATE MANUAL GLP AUTO OPTION IN MULTI MODE Autopace Automatic repeat dispensing at settable time interval with one trigger press AutoPace allows multiple aliquots of the same volume to be dispensed repeatedly at equal time intervals after the dispense trigger is pressed once In murti mode press and hold the mope key to reach the options menu Then click the mope key until auto appears on the display The first time used the display will flash orf Use the arrow keys to set the time interval between dispenses The range is 0 5 sec onds to 25 seconds in 0 1 second increments When the time interval is set press either trigger to pick up the set vo
6. idea to store the EDP3 Plus pipette on the stand when itis not in use This practice will provide a safe storage place and the EDP3 Plus will always be fully charged Note no charging takes place if the EDP3 Plus pipette is above approximately 90 of its charging capacity when connected to a charger When charging is complete the outer box on the battery symbol flashes once per second to show trickle charging is taking place A fully charged battery is indicated by the battery symbol dimming briefly every few seconds 20 Replacing the Battery ALL USER SETTINGS WILL BE LOST IF YOU REMOVE THE BATTERY To preserve your settings plug in the optional wall power supply cord before removing the battery Open the battery compartment by pressing upward with the thumb on the grooved area of the compartment door BATTERY COVER x CHARGING CONTACTS CHARGING CONTACTS UNDERSIDE SEEN FROM BELOW FIGURE 6 BATTERY COMPARTMENT AND CHARGING CONTACTS 1 Remove the old battery by lifting the unattached end slight ly and sliding it out Lift Slightly 71 BATTERY be L PLUG 2 Align the replacement battery connector plug with the socket in the battery compartment Slide the battery into place You will see all the display segments flash on briefly then hear EDP3 Plus set itself to zero a few seconds ofter you plug in the connector ALIGN PLUG WITH SOCKET AND SLIDE BATTERY INTO PLACE FIGURE 7 R
7. in premature seal wear and damage to the piston Exposure of internal compo nents to corrosive fumes can be reduced by using RAININ tips with aerosol barrier filters Service Calibration and Repair RAININ maintains two state of the art Repair and Calibration Service Centers in the United States RAININ Service Center 7500 Edgewater Drive Oakland CA 94621 USA Tel 800 662 7027 Fax 510 564 1683 RAININ Service Center RAININ Road Woburn MA 01801 USA Tel 800 662 7027 Fax 781 935 7631 Note for Customers outside the U S A Service is also available in many other countries through author ized RAININ distributors Visit the website www rainin global com to see a listing of RAININ distributors and service providers in your country or region Replacement Parts Parts for E3 Series 10 pL to 1000 pL A Shaft B Tip Ejector C Seal D O ring E Stroke Spring F Seal Retainer G Shaft Coupling E3 10 E3 20 E3 100 E3 200 E3 1000 6202 064 6202 065 6202 066 6202 229 6202 230 6202 071 6202 071 6202 073 6202 231 6202 232 6200 138 6200 143 6200 150 6200 154 6200 161 6200 139 6200 170 6200 151 6200 155 6200 162 6200 195 6200 197 6200 197 6200 199 6107 108 6200 196 6200 198 6200 201 6200 200 6107 106 6107 063 6107 063 6107 063 6107 063 6107 063 OQ 7 Io 00 gt 24 Replacement Parts cont d Parts for SE3 Series 10 uL to 1000 pL A Shaft B Tip Ejector C Seal D O ring E Stroke Spring F Seal Retainer G Shaft Coupling
8. moves to the left uu Silent alarm announced on display Day alarm silent set for 90 days Use ARROW key to set from 1 to 999 days j Add service entry Use ARROW key to toggle between Yes or No If Yes is selected Ade sr C 5 click the trigger to begin making an entry 27 The next screens show a service entry being made If Add was selected in the last screen click the trigger to open the next screen to set the date with the month number flashing j Use ARROW keys to select the month move to the next field with the trigger key then set the day and year the same way df 6 i62 j Finish the service entry by setting an ID value 01 to 99 with the up down ARROW keys fol mii lowed by pressing either triiger to complete the ut service entry id Making a service entry does two things a The current present cc and date logs are saved with the date and technician ID at the next lower menu level and b the current logs are zeroed and counting will start again when you start pipetting again As you cycle through the modes eLp mode show PrES the present set of logs i Press the DOWN ARROW at this screen to see the last saved set of logs BLP PrES Pressing the pown ARROw opens the last saved set level 1 which you can cycle through using Reset Then the pown arrow opens the next last set at level 2 and so on to 40 levels The up ARRow takes you to more recent entries Sample GLP Menu
9. the first sum volume then press the trigger repeatedly to dispense all the sequenced volumes Press RESET after the last volume in the sequence Then the display shows the pickup volume needed to complete the sequence in this case the sum of 34 uL and 22 pL cu WaT p Display shows second pickup needed to com SED plete the sequence dx Shu Press the trigger to pickup the second sum volume then press the trigger repeatedly to dispense all the sequenced volumes Press reset after the last volume in the sequence After volume sequencing has been invoked all sequential vol umes will be stored in memory Up to 16 volumes can be stored in a sequence Note sea and auto options can be used at the same time in muti mode DILUTE MODE In murti mode press and hold the mope key to reach the options and hidden modes click mope until piLuTe is reached then switch on DILUTE Mode with the ARRow key Press RESET and click mope until DILUTE is reached In DILUTE Mode EDP3 Plus picks up first the diluent n1 then an air gap CAir then the sample n2 and dispenses the diluent and sample all at once The first time piLute mode is used the n1 value is 25 of nominal volume and the n2 value is 50 of nominal volume The air gap is fixed Press the uP or DOWN ARROW until the desired value is shown for n1 the diluent volume PERF i ent DILUTE mode with 25L set for n1 the diluent Lactate volume Press the res
10. value only is shown 10 To see the sea index number during the pipetting sequence momentarily press reset enough times until the display shows one of the sequence index numbers at the pickup HOME posi tion then start the sequence The sea number will now show at Home and the cc number will show when the piston is away from HOME FP OPTION IN PIPET MODE Fixed Volume Pipetting up to six discrete volumes can be loaded for routine work or standard protocols In PIPET Mode press and hold the mope key to reach the options menu Then press the mope key repeatedly until FP is displayed If FP option is off switch it on with either arrow key then press and release the mope key The display will read FP1 with flashing o l in the volume area PEREP i PIPET Set the first fixed volume with the ARROW keys In this case 22 5 pL was set for FP1 FPI ee Sui PCP i PIPET Press MODE to scroll to the next fixed volume 5 a and set with the ARROW keys In this case FPe 4 isu 41 3 uL was set for FP2 row APET Press MODE to scroll to the next fixed volume and set with the ARROW keys In this case EPJ oe Su 82 9 uL was set for FP3 When the desired number of FP volumes have been set press RESET to begin pipetting with the volume set in FP1 In this mode normal operation of the arrow keys is suspended The UP ARROW scrolls through the fixed volumes in ascending order FP1 FP2 FP3 etc then rolls over after the last
11. volume at 0 0 pL and pret press RESET to begin volume sequencing in PIPET TF re nn j mode at LLL di When all volumes in the sequence have been set as shown above pressing Reset will let you begin volume sequencing in pipet mode With a tip attached and immersed in sample press either trigger to pick up the first volume then dispense that volume into a vessel using either trigger Press the trigger again to pick up the second volume in the sequence and dispense it with either trigger and so on After dispensing the last volume the sequence begins over Turn the sea option off by holding the mope key to open the options menu and pressing the mope key repeatedly until you reach the sea menu Then press either aRRow key Notes 1 Volume sequencing can be started from any point in the sequence by scrolling through volumes with the reset key then pressing either trigger at the desired starting volume 2 You can edit a previously stored sequence of for example 10 volumes to have only four volumes by setting new values as needed for the first four volumes then setting zero as the fifth value If at some future point you enter a non zero value as the fifth volume volumes 6 though 10 of your original stored sequence are recalled from memory 3 With sea and cc options both selected the same display seg ments are used to show both sequence index number and cycle count Normally in pipet mode with sea and cc both enabled the cc
12. EDP3 Plus Advanced Ergonomic Electronic Pipette a 9 pipettes for i volume ranges from 0 5 uL o 20 mL 200 uL EDP3 Plus shown RAININ TABLE OF CONTENTS IIMOCUCTION E is siia o aa avant suas aeaa aa a Aaa Liat Unpacking i e Set Up Initial Charge Sleep Mode Rapid Charge Stand Tip Selection Tip Immersion Depth Filloas nitial OP rdtiON eaire piai EEEE E ea Modes amp Options serien a a a Basic Operation PIPET and MuLTI modes PIPET MODE ci o a anior inn Ei MULTIDISPENSE MODE Switching on Hidden Modes and Options Options Common to All Modes SND OPTION sseeceeeseeeeeteeneeeees CC OPTION SPEED OPTION iiinn Advanced Modes and Options PIPETMODE Yaa tam a AE AA Cee E aAA A ATUNA a A PIPET CMIX OPTIONS oii ain n N E i EA SEQ OPTION IN PIPET MODE 2 FP OPTION IN PIPET MODE 1 MULTIDISPENSE MODE crsa dts cei cen beatin dhs a Adages ee 12 AUTO OPTION IN MULTIDISPENSE MODE l 12 SEQ OPTION IN MULTIDISPENSE MODE isce 12 DILUTEsMODE a rassa n EAA cate a lan AT Ta eA a a TaT re Wie AT 14 DILUTE BEMICOPTON irira aips eea EEA vin EARE 15 SEQ OPTION IN DILUTE MODE 0ssceecceecetteeeeetteeteeneestenteneeeents 16 TIRATE MODE a eonctis hain ESNEA E EEEO E E EEEE TNE nerd 17 MANUAL MODE ineine iina ae A R a 18 Battery charging Wall Power Supply s s s 20 Battery charg
13. EPLACING THE BATTERY 3 Replace the battery compartment door Battery life is dependent on such factors as m Pipette volume range m Full range or part range pipetting m Multidispensing 21 Tip Ejector Arm Removal The tip ejector can be removed if necessary Three types of tip ejector are used and all can be removed with minimum effori do not use force Up to 2000 pl 5000 yL 10 mL 20 mL Tet l l l FIGURE 8 REMOVING THE TIP EJECTOR ARM For models up to 2000 uL press in the quick release tabs on the ejector arm and pull the ejector down For 5000 uL amp 10 mL models grasp the top of the ejector arm and pull outward then downward For 20 mL models pull the lower part of the tip ejec tor away from the upper part do not remove the upper part To replace the ejector arm on all models except 20 mL insert the shaft through the large opening align the top with the tip ejector pushrod and push until the ejector arm snaps in place For 20 mL models place the large opening over the shaft and align the rod in the lower part of the tip ejector with the hole in the upper part and press firmly Care and Maintenance EDP3 Plus pipettes are sophisticated laboratory instruments and should be treated with appropriate care Designed to need very lit tle maintenance your EDP3 Plus should give years of trouble free service provided it is treated with proper care and the operating reco
14. In murti mode press and hold the mope key to reach the options and hidden modes click mope until TITRATE is reached then switch ON TITRATE Mode with the arrow key Press RESET and click mope until TITRATE iS reached Titration is simple with EDP3 Plus In titrate mode any volume up to full nominal volume can be aspirated and then titrated very accurately under trigger control PER TAT TITRATE mode showing full nominal volume mar y Change this volume with UP DOWN arrow keys LOLU Hi if desired 17 A settable fast dispense feature Fd allows rapid dispensing of the set Fd volume then titration under trigger control as described above Pressing RESET once twice if cc option is also on allows you to set the fast dispense volume if desired tae Taal ql p Fast dispense volume of 500 pL set Fd cow Press either trigger to begin titration The set aspirate volume is picked up Pressing and releasing either trigger first dispenses the Fd volume if set then titrates the EDP3 Plus s smallest increment 1 1000th of the nominal volume Holding the trigger titrates the sample at increasing speed until the trigger is released again THAT DEPEMSE 1 Display always shows actual volume titrated Gc Su MINSI DGPEMSE 1 feted fT Trigger pressed and released to titrate a single incremental volume MINST DEFENSE 1 FSu Trigger pressed and released to titrate a single incremental volume MANUAL MODE In muLTI mode pre
15. Logs The following table is a representation of 6 sample sets of GLP logs Each field of the table is displayed one at a time on the LCD To navigate through the entries use the reset key to move to the right and the uP Down arrow keys to move vertically At any point in the eLp logs pressing and holding reset takes you to the eLp PrES position Cycle count Cycle Days since since count last service Tech Log Set last service total service date ID GLP PrES SEr 320 ALL 12 dAS 2 n a GLP 1 SEr 2617 ALL 11 6 15 2 02 GLP 2 SEr 2524 ALL 9 4 15 2 02 GLP 3 SEr 2491 ALL 6 3 15 2 02 GLP 4 SEr 2605 ALL 4 2 15 2 02 GLP 5 SEr 1572 ALL 1 1 15 2 02 28 EC Declaration of Conformity according to ISO IEC Guide 22 and EN45014 Manufacturer s Name Rainin Instrument LLC Manufacturer s Address 7500 Edgewater Drive Oakland CA 94621 USA declares that the following product Product Name EDP3 Plus Electronic Motorized Microliter Pipette Model Number EDP3 Plus Product Options E3 RCS Rapid Charge Stand E3 WPS Wall Power Supply conforms to the following Product Specifications Safety EN61010 1 1995 EC1010 1995 Al A2 EMC EN61326 04 1997 Emissions EN55011 1998 CIRSPR 11 1997 Class A Conducted Emissions and Radiated Emissions EN61326 04 1997 Immunity EN61000 4 2 1995 IEC6100 4 2 A1 1998 01 ESD Immunity N61000 4 3 1996 RF Immunity 80 100MHz NV50204 1996 RF I
16. SE3 10 SE3 20 SE3 100 SE3 200 SE3 1000 A 6200 140 6200 145 6200 147 6200 382 6200 383 B 6200 133 6200 144 6200 148 6202 231 6202 232 C 6200 138 6200 143 6200 150 6200 154 6200 161 D 6200 139 6200 170 6200 151 6200 155 6200 162 E 6200 195 6200 197 6200 197 6200 199 6107 108 F 6200 196 6200 198 6200 201 6200 200 6107 106 G 6107 063 6107 063 6107 063 6107 063 6107 063 Parts for E3 and SE3 series 2000 yL to 10 mL A Piston O ring B Cylinder C Cylinder O ring D Shaft E Tip Ejector F Seal G Stroke Spring H Seal Retainer 2000 pL 5000 pL 10 mL A 6200 167 6107 112 6107 113 B n a 6200 365 6200 371 c n a 6200 364 6200 370 D E3 Series 6202 214 6202 222 6202 223 D SE3 Series 6200 169 6200 362 6200 368 E 6200 168 6200 373 6200 374 F 6200 166 n a n a G 6107 109 n a n a H 6107 107 n a n a Common parts for 5000 pL and 10 mL E3 and SE3 series Tube of grease Filters 6100 555 6190 164 pack of 100 6190 165 pack of 1000 Parts for E3 20ML Piston O ring Cylinder Cylinder O ring Shaft Tip Ejector Tube of grease Filters 6202 299 6202 301 6202 300 6202 302 6202 298 6100 555 6190 221 pack of 100 6190 222 pack of 500 Parts common fo all EDP3 Plus models Battery Battery Cover Wall Power Supply US Wall Power Supply UK Wall Power Supply EUROPE Wall Power Supply JAPAN 6107 040 6107 085 E3 WPS E3 WPS240V E3 WPS220V E3 WPS100V 25 Specifications These manufacturer s specifications shou
17. ast Auto Autopace automatic repeat dispensing at settable time interval after trigger press SND Sound on ofF voLuME 1 Soff fo 7 loud GLP Good Laboratory Practice user logs The next few pages describe 1 basic operation of EDP3 Plus in PIPET and MuLTI modes without options 2 switching on advanced modes and 3 advanced operation using all modes and options Basic Operation PIPET amp MULTI modes EDP3 Plus is shipped ready for basic operation PIPET and MULTI modes are switched on other modes are hidden The hidden modes can be switched on as needed in murti mode described later PIPET MODE Select Volume with Arrow Keys ARROW keys operate when the piston is at the zero point ready to pick up sample The DOWN arrow reduces and the uP ARROW increases the volume Touch either ARROW key to change volume by one rice j increment Press and hold the ARRow PFET i i nan key the volume changes while you 90 hold down the key slowly at first then with increasing speed Volume Scrolling While pressing the arrow key note the changing volume setting pauses at 25 50 and 75 of the pipette s nominal volume then continues at the faster rate At maximum volume pressing the uP ARRow rolls the volume over fo begin again at the minimum volume 0 5 of maximum vol ume Pressing the Down arrow at the minimum volume rolls over to the maximum volume This allows rapid volume change from high to low or vi
18. attery for about 15 min utes initially you can use EDP3 Plus while it is charging Connect the wall power supply to a power outlet matching the line voltage printed on the label 120 VAC 60 Hz US 220 VAC 50 Hz Europe 240 VAC 50 Hz UK 100 VAC 50 Hz Japan Connect the other end of the cord to the power socket on the back of EDP3 Plus After 15 minutes the pipette will be charged enough for a typical day s pipetting and fully charged after approximately 60 minutes The battery symbol outline in the top right corner of the display flashes while charging Sleep Mode To extend battery life the EDP3 Plus display sleeps affer fen minutes of inactivity i e ten minutes after the last keystroke or trigger press Press any key or trigger fo wake the display Rapid Charge Stand E3 RCS Rapid Charge Stand charges three EDP3 Plus pipettes in sequence With one EDP3 Plus on the stand charging proceeds as if the wall power supply were connected directly to the pipette But when charging more than one EDP3 Plus the one placed on the stand first will be charged first then the others sequentially To charge a particular pipette remove other pipettes and place the desired pipette in the stand See page 20 for more information Tip Selection To ensure proper leak free fit and conformance to specifications use only RAININ tips with EDP3 Plus pipettes When loading tips press the EDP3 Plus shaft into the end of the tip with only suf
19. ce versa If you roll over through the high or low values the display pauses briefly at the maximum and minimum volumes as it rolls over Aspirate or Dispense with Trigger When the desired volume is set attach a tip press the shaft into the end of the tip with only enough force to make a positive seal Immerse the tip end into the sample and touch either trigger to aspirate To dispense touch the tip end against the vessel side wall and press either trigger After blowout remove the tip from the vessel and press the tip ejector button to discard the used tip Press either trigger once to aspirate then press either trigger again to dispense You can even press both triggers at once to aspirate and dispense use the trigger which is most convenient MULTIDISPENSE MODE 1 Press mope button until pickup muti shows on the LCD 2a Set aliquot size using either ARRow KEY EDP3 Plus automati cally computes the number of aliquots that can be dispensed and shows the number 8x 6x etc in the lower left of the display When aliquot siize is set and trigger is pressed EDP3 Plus aspirates the maximum volume of sample need ed To ensure accuracy on the last aliquot EDP3 Plus always picks up slightly more sample than will be pipetted PERF WTI j MULTIDISPENSE mode selected on a 200 pL EDP3 Plus with 50 yL aliquot volume nna We 5 LLLI Jil OR 2b Select number of aliquots with RESET ARRow keys Press either ARROW KEY to se
20. d 6190 222 pack of 500 Initial Operation Before initial operation you should charge EDP3 Plus for about 15 minutes as described on page 2 Before pipetting for the first time take time to familiarize yourself with the key functions and practice scrolling through modes and options as described on the following pages The first time the EDP3 Plus pipette is used it will default to Piper mode If not press the mope key until pipet shows on the LCD see below Per Battery Level Indicator oot wi LCD Liquid Crystal Display MODE KEY z UP ARROW RESET KEY E DOWN ARROW DUAL ACTION TRIGGERS FIGURE 3 EDP3 PLUS INTERFACE 200 uL MODEL SHOWN Modes amp Options EDP3 Plus has six modes of operation with several options per mode listed below BASIC MODES OPTIONS AVAILABLE PIPET amp MIX SEQ FP CC SPEED MULTI AUTO SEQ SPEED SND ADVANCED MODES SMTH ON AND CFF IN MULTI MODE DILUTE amp MIX SEQ CC SPEED TITRATE CC SPEED MANUAL CC SPEED GLP SEE APPENDIX A Abbreviations amp mix Mix Piston moves up and down as trigger is held mix ing the contents of the tip SEQ Volume Sequencing a sequence of different volumes is aspirated or dispensed depending on mode FP Fixed Point up to six discrete volumes can be pro grammed for routine work or standard protocols cc Cycle Counter each pipetting cycle is counted sPEED Aspiration Dispense speed 1 slow to 10 f
21. d to set fifth sequen sm mue i f inn tial volume of 100 pL n HOG H lie UP DOWN arrow keys used to set last sequential n n volume by entering O mim Ube Press either trigger to begin operation Each sequential volume is picked up in turn by another trigger press Then the display will show the addition of all the volumes ready to dispense CRPEHSE yoo Total of all sequence volumes shown ready to dispense mi f zi Press either trigger to dispense all dilution sequence volumes Notes 1 If you included air gaps in the sequential volume settings the value displayed may not reflect the actual volume of liquid in the tip You must subtract the addition of all air gaps from this total 2 sea and mix options can be used together in piLutE mode With sea and mix option both selected in piLute mode opera tion is the same as described above for pi ute with sea option except that pressing and holding the trigger when dispensing the tip contents first dispenses the tip contents then mixes the mix volume in the tip Releasing the trigger stops mixing and dispenses the tip contents 3 If sea and cc options are both selected the same display segments are used for both options In piLute mode the cc value is shown at the pickup home position and the sea index number is shown during aspira tion Then the cc number is shown during dispense and increments as the piston reaches the home position TITRATE MODE
22. equencing in pipet mode a sequence of up to 16 vol umes is picked up and dispensed In PIPET mode press and hold the mope key to reach the options menu Then press the mope key repeatedly until sea is displayed Turn this option on using either Arrow key Then press the RESET key to enable the sequential volumes to be set The first time sea option is invoked the display shows n1 with the EDP3 Plus nominal volume as a default Change this volume as needed with the arrow keys then use reset to scroll though the sequence volumes setting values with the arrow keys The fol lowing example setting three sequential volumes assumes that volume sequencing has not been invoked before After volume sequencing has been invoked all sequential volumes will be stored in memory Up to 16 volumes can be stored in a sequence 9 PERY Display shows n1 first sequential volume set PPET at the nominal pipette volume in this example BEC 100 pL Gann af POLE H First volume is reset to 50 pL with ARROW keys een When editing a sequence the volume flashes Me c nn and the PICKUP symbol disappears Press Li LLL RESET to reach the next volume APET Second volume is changed from 0 0 pL to 25 pL BED aa with ARROW keys Press RESET to reach the next ae CSO yy vme PIPET Third volume is changed from 0 0 pL to 12 5 pL BEC with ARROW keys Press RESET to reach the next i aa l g5 ul volume r To finish the series leave
23. et key fo allow n2 the sample volume to be set with the UP Or DOWN ARROW PERE i Sample volume n2 set to 75pL ne 7 Gul When both diluent and sample volumes have been set press RESET fo begin operation in pilute mode Press either trigger to pick up the diluent volume Then lift the tip from the diluent and press the trigger again to pick up a fixed volume of air PERF Fixed volume of air is picked up to separate the diluent and sample Place the tip end into the sample and press either trigger again to pick up the sample eee volume Now the diluent air gap and sam AIR GAP ple are in the tip and the display shows the total volume of the diluent and the sample volumes SAMPLE DISPENSE niina Total volume of diluent and sample nnn iLL To dispense the diluent and sample together press either trigger DILUTE mode can also be used with the following options amp MIX CC SEQ SPEED DILUTE amp MIX OPTION Piston moves up and down mixing tip contents In DILUTE Mode press and hold the mope key to reach the options menu Then click the mope key until amp mix appears on the display The first time used the display will flash orf Use the arrow keys fo set the volume to be mixed Allowable range is from 5 to 100 of the nominal volume row i aug DILUTE mode with MIX option set to mix 100 pL mu same as sample plus diluent in this case nn PLL When the mix volume is set operation
24. fi cient force to make a positive seal Tip Immersion Depth Recommended depths for tip insertion into sample are shown below EDP3 Plus Volume Range Immersion Depth 10 uL 0 5 10 uL 1 2 mm 20 uL 2 20 uL 2 3 mm 100 uL 10 100 uL 2 3mm 200 uL 20 200 uL 2 4mm 1000 uL 100 1000 uL 2 4mm 2000 uL 200 2000 uL 3 6mm 5000 uL 500 5000 uL 3 6mm 10 mL ImL 10 mL 4 10mm 20 mL 2mL 20 mL 4 10mm Tip immersion depth is important If these depths are exceeded the volume measured may be inaccurate possibly out of specifi cation Tip angle hold EDP3 Plus within 20 degrees of vertical 2 Filter EDP3 Plus 5000 uL 10 mL and 20 mL pipettes use a filter in the end of the shaft to help prevent liquid entering the shaft and con faminating the piston Using such a filter is important when pipet ting large volumes Replace the filter if it gets wet End of End of End of 5000 pL 10 mL 20 mL Shaft Shaft Shaft Filter Filter Filter FIGURE 2 FILTER ORIENTATION The 5000 uL and 10 mL pipettes use the same filter oriented as shown in the diagram above For 5000 yL insert the small diam eter into the shaft For 10 mL insert the large diameter into the shaft Filter part numbers are 6190 164 pack of 100 and 6190 165 pack of 1000 The filter for 20 mL is a cylinder which can be installed in either orientation Filter part numbers 6190 221 pack of 100 an
25. he display reads O PERY j PIPET CC OPTION selected with 25 pL set volume and 43 cycles counted n 43 Sow To start the count at any cycle number press RESET momentarily This allows the desired cycle number to be set with arrow keys SPEED OPTION Aspiration dispense speed 1 slow to 10 fast EDP3 Plus is delivered with the speen option set to 10 This set ting can be changed for any mode as desired High speeds 9 or 10 are best for aqueous samples and slower speeds are useful for viscous foaming or shear sensitive samples PERF i PIPET SPEED set to 10 in PIPET mode DPED mn Hu In any mode press and hold the mope key to reach the options menu Then press the mope key repeatedly until speen is dis played Use the arrow keys to set the desired speed The maximum speeds of larger volume models are limited to pre vent fountaining or air intake when aspirating Large volume maximum speeds 5000 uL 8 10 mL 8 20 mL 6 SPEED FULL SCALE FULL SCALE DELAY BEFORE BLOWOUT HOLD AT END SETTING PICKUP DISPENSE BLOWOUT DURATION CONSTANT 10 0 7 s 0 7 s 0 0 s 0 13 s 1 0s 9 1 0s 1 0s 0 4 s 0 22 s 1 0s 8 15s 1 58s 0 6 s 0 30 s 1 0s 7 1 9 s 1 9 s 0 8 s 0 38 s 1 0s 6 24s 24s 0 9 s 0 50 s 1 0s 5 2 85 2 85 1 1 s 0 52 s 1 0s 4 3 2 s 3 2 s 1 58 0 58 s 1 0s 3 3 8 s 3 8 s 1 7 0 69 s 1 0s 2 4 55s 45s 1 9s 0 80 s 1 0s 5 3 s 5 3 S 2 5 S 1 04 s 1 0s
26. ing Rapid Charge Stand 20 Replacing the Battery 0 ccc cece eeseeeeeeeeteeeeteeteeetees 21 Tip Ejector Arm Removal ireren 22 Care and Maintenance en 22 AUIOCIOVING i s a E A atnieaio occaconte arose T 23 Troubleshooting and Repairs 23 Service Calibration and Repoir s ee enteeees 24 Replacement Parts ccccccceseeeeteeeceeeteeettteeteeeeneeteeneees 24 Specifications Electrical specifications 0 cc cece 26 ADPe Ndix A GLP MODE 7ra anei e aea ae E raaa ETA 27 EC Declaration of Conformity 0 0 0 cee Inside Back Cover Contacting ROININ cece cece ete eteeteeeteees Back Cover FIGURES Figure 1 200 uL EDP3 Plus Pipette 2 0 0 eee eeeeeeeeee Figure 2 Filter Orientations jc wacie erer 3 Figure 3 EDP3 Plus Interface Maid Figure 4 Measuring Unknown VolUME 0 ccceeeeeeees 19 Figure 5 Charging Devices 20 Figure 6 Battery Compartment and Charging Contacts 21 Figure 7 Replacing the Battery 21 Figure 8 Removing the Tip Ejector AM 0 cece 22 This device EDP3 Plus is intended for use only as an electronic pipette for pipetting liquids as described in this manual It is not intended for any other use Note that pipette specifications and other information in this manual may be changed without prior notice EDP EDP3 EDP3 Plus and LTS are trademarks of Rainin Instrument LLC 1999 2002 Rainin Instrument LLC All rights rese
27. is the same as described for piLute except that pressing and holding the trigger dispenses the tip contents then mixes the mix volume in the tip Releasing the trigger stops mixing and dispenses the tip contents Alternately a single touch to the trigger dispenses completely with out any mixing occurring SEQ OPTION IN DILUTE MODE Volume sequencing in piLute mode a sequence of up fo 16 vol umes can be aspirated then dispensed all at once In DILUTE mode press and hold the mope key to reach the options menu Then press the mope key repeatedly until sea is displayed Turn this option on using either Arrow key Then press the RESET key Use the up pown arrow keys to set values for the sequenced volumes Note there is no air setting when using the sea option but any sequence volume can be used for an air gap DILUTE mode with SEQ option 100 pL set as first sequential volume Press RESET and then use the up pown arrow keys to set the next volume in the series 1 UP DOWN arrow keys used to set second BEG PL om sequential volume of 50 pL ne Li Continue using the ReseT and up pown arrow keys to set the remaining volumes in the series Finish the series at any point by entering O as the value 1 UP DOWN arrow keys used to set third sequen tial volume of 100 pL E me nn nJ LL pl gt UP DOWN arrow keys used to set fourth sequen BED i tial volume of 50 pL Li c A J na LL ae UP DOWN arrow keys use
28. ld be used as guidelines when establishing your own performance specification in accor dance with ISO 8655 EDP3 Plus Specifications Volume Increment Accuracy Precision Model uL uL uL uL lt 10 pL 1 0 01 2 5 0 025 1 2 0 012 5 1 5 0 075 0 6 0 03 10 1 0 01 0 4 0 04 20 uL 2 0 02 7 5 015 2 0 0 04 10 1 5 0 15 0 5 0 05 20 10 0 2 0 3 0 06 100 pL 10 0 1 3 5 0 35 1 0 0 1 50 08 04 0 24 0 12 100 08 08 0 15 0 15 200 uL 20 0 2 25 05 1 0 0 2 100 08 08 0 25 0 25 200 08 16 0 15 03 1000 pL 100 1 0 3 0 3 0 0 6 0 6 500 08 40 0 2 1 0 1000 08 80 0 15 1 5 2000 uL 200 2 0 3 0 60 0 6 1 2 1000 08 80 0 2 2 0 2000 08 16 0 0 12 24 5000 uL 500 5 0 24 12 0 0 6 3 0 2500 0 6 15 0 0 2 5 0 5000 0 6 30 0 0 16 80 lOmL 1mL 10 0 5 0 50 0 0 6 6 0 5 mL 1 0 50 0 0 2 10 0 10 mL 08 80 0 0 16 16 0 20mL 2mL 20 0 5 0 100 0 0 60 12 0 10 mL 1 0 100 0 0 20 20 0 20 mL 0 8 160 0 0 16 32 0 Specifications are subject fo change without notice Electrical specifications This device is intended for use only with the power sources with RAININ part numbers listed below No other power sources may be used with this device Wall Power Supply Input P N E3 WPS 120VAC 60 Hz P N E3 WPS220V 220VAC 50 Hz P N E3 WPS100V 100VAC 50 Hz P N E3 WPS240V 240VAC 50 Hz Wall Power Supply Output All P Ns 5 5VDC 1 04 A Regulated Nominal Battery P N 6107 040 Li lon 3 6 VDC Nominal 400mAh Nominal Explanation of symbols A Ampere Hz Hertz mAh Milliamp Hour
29. ling and carefully remove the shaft Note how the parts fit onto the piston Inspect the seal assembly and piston for contamination The piston should be shiny and free of corrosion Clean with dis tilled water or isopropyl alcohol Dry with a lint free tissue and reassemble after inspecting the interior of the shaft for any contamination If piston corrosion or staining is evident do not use the pipette Call 800 662 7027 for Pipette Service Leaks Inaccurate Sampling Abnormal Stroke 1 2 Loose shaft Tighten coupling by hand Split or cracked shaft Remove the tip ejector and inspect the shaft Replace the shaft if necessary If the shaft was dropped remove it to see if the piston is bent If so return the instru ment for service Worn seal and or o ring Models below 5000 pL use a poly ethylene seal and o ring Check the seal and o ring replacing them as necessary To replace pull off the old seal and o ring position the new seal and o ring on the piston assembly and reassemble the pipette Do not lubricate any components EDP3 Plus has a dry seal except for 5000 uL 10 mL and 20 mL models which use a small amount of grease on the seal 23 After pipetting concentrated acids or highly corrosive solutions disassemble EDP3 Plus and inspect and clean the piston assem bly shaft and seal with distilled water Dry all components thor oughly and reassemble Extensive contact with corrosive fumes may result
30. lume Then press the trigger again to begin dispensing auto matically at the set time interval Pressing either trigger pauses autodispensing until a trigger is pressed again when autodis pensing restarts At the end of the last dispensed aliquot press RESET fo begin the next pickup MULTIOISPENSE AUTO j Autodispensing set in MULTI mode Note AUTO symbol flashes when paused and is uu 5 nn steady when active ck i LIL He SEQ OPTION IN MULTI MODE Volume Sequencing in MULTIDISPENSE Mode a sequence of up to 16 volumes is dispensed from a pickup In mutt mode press and hold the mope key to reach the options menu Then press the mope key repeatedly until sea is displayed Turn this option on using either Arrow key Then press RESET The first time sea option is invoked the display shows 1x with the nominal full volume of the pipette model To set sequential volumes press RESET fo scroll though the sequenced volumes setting values with the arrow keys PERY WAT Display showing the SEQ option invoked for the EG first time on a 1000 pL model E3 ONNAN w MULL d Press RESET to begin volume sequencing in MULTIDISPENSE mode The display shows the addition of all volumes to be sequenced and the number of times the total volume can be picked up and the sequences dispensed WAT First volume is set to 275 pL with ARROW keys im When editing a sequence the volume flashes tag 2 j 5 and the PICKUP symbol disappears Press
31. ma iIi RESET to reach the next volume WATI Second volume is set to 125 uL Press RESET to Ser reach the next volume ae ie Sw WTI Third volume is set to 85 pL Press RESET to BE reach the next volume nJ GSu WLT Fourth volume is set to 55 pL Press RESET to SED reach the next volume ad SS WaT To finish the series leave volume at 0 0 uL and BEG m press RESET to begin volume sequencing in a 5 U jil MULTIDISPENSE mode Press either trigger to pick up the total volume Press either trigger again to dispense the first volume Continue pressing and releas PERY WLT Sum of sequenced volumes and number of times SED the total volume can be picked up ly SYOw ing a trigger to dispense all volumes sequentially After the last volume in the sequence is dispensed display shows Ox with RESET flashing press RESET fo start the next pipetting cycle If the sum of the sequential volumes is more than the full scale pickup volume of the pipette the seqential volume series is split into two or more pickups This situation is shown by a horizontal dash in front of the number of pickups ric MULTI 1 3 R Display shows that more than one pickup is SEG needed for the sum of the sequenced volumes In the example the sum of the sequenced volumes is 1045 The first pickup volume is 989 sum of all sequence volumes that can be picked up which must be less than the pipette s volume Press the trigger to pick up
32. mmendations in this manual are followed The most important factor in taking proper care of the EDP3 Plus pipette is to keep the mechanism dry and clean The following simple rules should be strictly observed 1 Never allow liquid to enter the shaft where it can contact the piston or seal 2 Never pick up liquid without a tip attached 3 Never invert the EDP3 Plus pipette or lay it on its side with liq uid in the tip Always hold it upright and store it upright if pos sible The Rapid Charge Stand can be used to hold and charge three EDP3 Plus pipettes 22 Never use solvents to clean EDP3 Plus Instead use a lint free wipe dampened with water to clean the instrument Keep the keyboard display dry Never attempt to recharge EDP3 Plus with any other device than either the RAININ Model E3 WPS Wall Power Supply or the Model E3 RCS Rapid Charge Stand Severe damage to the internal electronics would result Autoclaving Autoclavable parts of the Model EDP3 Plus are the shaft and the tip ejector 121 C 1 bar 15 20 minutes Do not autoclave the complete EDP3 Plus pipette or any parts other than the shaft and tip ejector Troubleshooting and Repairs Warning On models from 10 200 uL when removing the shaft from the body ensure the spring seal and o ring do not fall off the piston Sample Splash liquid inside the mechanism l 2 Refer to Figure 8 to remove the tip ejector arm Unscrew the shaft coup
33. mmunity 900 MHz N61000 4 4 1995 Electrical Fast Transient Immunity N61000 4 5 1995 Surge Immunity N61000 4 6 1996 Conducted Immunity EN61000 4 11 1994 08 Supplementary Information Responsible Signatory Jim Petrek VP R amp D Dote April 22 2002 This Declaration of Conformity applies only to products which have the CE mark attached WARNING This equipment generates and uses radio frequency energy and if not installed and used properly i e in strict accordance with the instruction manual may cause harmful interference to radio communications It has been tested and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC rules which are designed to provide reasonable pro tection against such interference when operated in a commercial environment Operation of this equipment in a residential area is likely to cause interference in which case the user at his own expense will be required to take whatever meas ures may be required to correct the interference Limited Warranty See the enclosed Limited Warranty and Limitations of Liability Statement Please complete and return the Warranty Registration Card on receipt of your pipette RAININ pipettes are calibrated with RAININ tips To assure excellent reproducibility and performance use only RAININ tips as recom mended in this manual Specified performance is guaranteed only when RAININ fip
34. ount of sample in the tip To dispense press the DISPENSE trigger To complete the blowout stroke double click the DISPENSE trigger or click RESET Measuring Unknown Volumes in Manual Mode Set the maximum volume with the arrow key Immerse the tip end into the unknown volume Press either trigger to start aspirating and measuring The display will set to zero and then increase while you hold the trigger and EDP3 Plus will begin aspirating Click or press and hold the trigger until the complete volume to be measured is inside the tip If you overshoot and pick up some air after the liquid just click the other trigger DISPENSE until the air is expelled and the liquid is level with the tip orifice When all the liquid is in the tip with no air visible the display shows the exact volume of the liquid picked up upy A Pick up unknown B Display shows the volume until it is all volume picked up in tip a FIGURE 4 MEASURING UNKNOWN VOLUME The display can be zeroed with liquid in the tip if desired e g to measure a volume of one sample then a volume of another Press RESET for more than one second or press the DOWN ARROW to zero the display after measuring the first volume Then press a trigger to measure the second pickup Press the up arrow to see the total of the two measurements GLP MODE Refer to Appendix A GLP Mode in the back of this manual Battery Charging Wall Power Supply Unlike other types of battery
35. rved EDP3 Plus Pipettes are manufactured under U S patents 4 671 123 4 905 526 5 187 990 5 614 153 6 254 832 B1 and 6 299 841 LTS LiteTouch Tip Ejection System is protected by U S patents 6 168 761 B1 and 6 171 553 B1 Other U S and national patents pending 2 Introduction EPD3 Plus electronic pipette is an enhanced version of the original RAININ EDP3 It offers advanced operation with additional modes and options not found in the original EDP3 Model DISPLAY a 4 KEY PAD Te T e DUAL TRIGGERS E TIP EJECTOR BUTTON lt eee suas FINGER HOOK IMPACT RESISTANT BODY 3 E SHAFT TIP EJECTOR ARM 3 TIP FIGURE 1 200 uL EDP3 PLUS PIPETTE Unpacking The EDP3 Plus package should contain 1 EDP3 Plus Electronic 4 Performance Check Report Pipette with Battery Warranty Card 2 Operator Manual 5 Sample Tips 3 Quick Reference Guide The Wall Power Supply or the Rapid Charge Stand one of which MUST be purchased to recharge the EDP3 Plus battery are shipped in a separate box Unpack and check the contents against this list If anything is miss ing call Technical Service 800 543 4030 If any damage is evident file a claim with the shipping carrier who is responsible for damage incurred in transit Save the shipping packages if you file a claim Set Up Initial Charge EDP3 Plus is shipped with the battery charged But as it may have been stored before delivery charge the b
36. s are used Contacting RAININ Technical Information Phone 800 543 4030 Fax 781 938 1152 E mail fech support rainin com Pipette Service Phone 800 662 7027 Fax 781 935 7631 E mail service rainin com Direct Order Line Phone 800 472 4646 Fax 781 938 1152 E mail pipets rainin com Web Wwww rainin com From outside the U S Massachusetts Office 1 781 935 3050 California Office 1 510 564 1600 RAININ Japan Phone 03 5689 8311 Fax 03 5689 2670 Other International Offices and Distributors See www rainin global com E mail global rainin com from outside the U S Web www rainin global com from outside the U S RAININ Rainin Instrument LLC 7500 Edgewater Drive Oakland CA 94621 3027 a METTLER TOLEDO Company Prices and specifications are subject fo change without notice 150 Copyright 2000 2002 Rainin Instrument LLC CERTIFIED 9920 235 Rev D
37. ss and hold the mope key to reach the options and hidden modes click mope until MANUAL is reached then switch On MANUAL mode with the Arrow key Press RESET and click MODE until MANUAL is reached Using the arrow keys select the maximum sample size you wish to pipette The first trigger you touch is the aspirate trigger and the other becomes the DISPENSE trigger wa j MANUAL mode selected with 75 uL as the set pickup volume 30A iU To pick up the set volume immerse the tip into sample and press and hold the aspirate trigger This resets the display to zero then the volume increases until the set volume is picked up Press and hold the pDIsPENsE trigger until the desired volume is dispensed To blow out any remaining sample press the Reset key momentarily or if at Home position double click the dispense trigger You can also pick up or dispense one increment at a time by momentarily pressing the appropriate trigger After setting maxi mum sample size and immersing the tip into sample the first time you press and release the aspirate trigger the display will Zero then show the smallest possible increment of volume for the par ticular EDP3 Plus model Then each time you press the aspirate trigger momentarily one increment of sample is drawn into the tip If you press and hold the trigger the aspiration rate will increase to the preset speed When you release the trigger aspiration will stop The display always reads the actual am
38. t aliquot size Then set the total number of aliquots you wish to dispense by momentorily pressing RESET When the display flashes press the ARRow KEY to set the num ber of aliquots you wish to dispense Note you cannot increase the number of aliquots above the default maximum Press the trigger to enter the settings and start the aspiration EDP3 Plus will pick up only the amount of sample necessary to accurately dispense the number of aliquots selected 5 After aspiration each time you press a trigger one aliquot is dis pensed Touch off the droplets from each tip against the vessel wall fo ensure accuracy as each aliquot is dispensed PoP WTI j MULTIOEPEHSE j Aan an ty 45 LLLI il 3 500 il Multidispense Pickup Multidispense Dispense The counter will count down to zero and reset will flash on the dis play Press the Reset key or double click either trigger to expel the extra liquid remaining in the tip In mukti mode you can dispense all the liquid in the tip at any time by pressing RESET Switching on Hidden Modes and Options As shipped EDP3 Plus will operate in basic configuration Clicking the mope key toggles between pipet and murti modes Advanced modes DILUTE TITRATE MANUAL nd GLP are hidden Click the mope key to reach murti mode then press and hold the MODE key to reach the options and hidden modes Click move repeatedly to cycle through the options available in MULTI Mode SPEED AUTO SEQ
39. the Li lon battery in the EDP3 Plus has no memory effect and does not need to be fully discharged every month or so It will provide up to 3000 full stroke cycles fewer in large volume range models before needing to be recharged To recharge EDP3 Plus connect the wall power supply fo a power outlet matching the line voltage printed on the label 120VAC 60Hz US 220VAC 50Hz Europe 240VAC 50Hz UK 100VAC 50Hz Japan Connect the other end of the cord to the power socket on the back of EDP3 Plus After 15 minutes the instrument will be charged enough for a typical day s pipetting and fully charged after approximately 60 minutes The battery symbol outline in the top right corner of the display flashes while charging eal a Ee EDP3 Plus can be used while charging FIGURE 5 CHARGING DEVICES E3 WPS Wall Power Supply left and E3 RCS Rapid Charge Stand right Battery Charging Rapid Charge Stand E3 RCS Rapid Charge Stand will charge three EDP3 Plus pipettes one at a time in sequence Charging contacts are located under the pipette head and shown in the diagram in Figure 6 With only one EDP3 Plus on the stand charging will proceed as if the wall power supply were connected directly to the pipette However when charging more than one EDP3 Plus the first one placed on the stand will be charged first To charge a particular pipette remove other pipettes and place the desired pipette in the stand It is a good
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