Home

Agilent 1220 Infinity LC System

1. 174 1220 Infinity LC Preventive Maintenance and Repair 6 Autosampler 5 Install the new rotor seal and isolation seal Ensure the 6 Install the stator ring with the short of the two pins facing metal spring inside the isolation seal faces towards the towards you at the 12 o clock position Ensure the ring valve body sits flat on the valve body M 7 Install stator head Tighten the bolts alternately two turns at a time until the stator head is secure 1220 Infinity LC 175 6 Preventive Maintenance and Repair Next Steps 9 Slide the waste tube into the waste holder in the leak tray 10 On completion of this procedure Install the front cover 176 1220 Infinity LC Preventive Maintenance and Repair Autosampler Exchanging the Metering Seal When Tools required Parts required Preparations Poor injection volume reproducibility Leaking metering device 1 4 inch wrench supplied in the tool kit 4 mm hex key supplied in the tool kit 3 mm hex key supplied in the tool kit p n Description 1 5063 6589 Metering seal pack of 2 for 100 pl analytical head 1 5063 6586 Plunger Select Start in the Tools function in the LMD Software Change piston Remove the upper front cover assembly 1 Remove the two capillaries from the metering head 2 Remove the two fixing bolts and pu
2. 158 1220 Infinity LC Preventive Maintenance and Repair 6 Solvent Delivery System 3 Disconnect the DCGV cable unscrew the two holding 4 Place the new DCGV into position Make sure that the screws and remove the valve valve is positioned with the A channel at the bottom right position Tighten the two holding screws and connect the cable to its connector Channel A 5 Replace the DCGV cover Reconnect the waste funnel 6 Reconnect the tube from the inlet valve to the middle with the waste tube holder in the top cover Insert waste position of the DCGV and then the solvent tubes at tube in the holder in the waste pan and clip tube to the channel A and B of the DCGV DCGV cover Solvent tubes e EU Petit 17 2 4 Connecting tube DCGV to PIV B c amp E P 1220 Infinity LC 159 6 Preventive Maintenance and Repair Manual Injector Overview of Maintenance Procedures Table 41 Overview of Maintenance Procedures Procedure Typical Frequency Time Required Flushing the injector After using aqueous buffers or 5 minutes salt solutions Exchanging the injection valve After approximately 10000 to 10 minutes seal 20000 injections or when the valve performance shows indication of leakage or wear Flushing the Manual Injector CAUTION The use of aqueous buffers or salt solutions can lead to crystal formation Crystal formation may cause scratches on the in
3. CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 224 1220 Infinity LC Appendix 9 Solvent Information 1220 Infinity LC Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Prevent any crystallization of buffer solutions This will lead into a blokkage damage of the flow cell If the flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents e g Acetonitrile or Methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive again
4. 1 Locate the cuvette holder on the desk 2 Unscrew the bracket 192 1220 Infinity LC Preventive Maintenance and Repair 6 3 Insert the cuvette with the sample into the holder The clear side of the cuvette must be visible Light path Clear side Reset the lamp counter as described in the User Interface documentation Turn the lamp ON Give the lamp more than 10 minutes to warm up Perform Wavelength Verification Calibration to check the correct positioning of the lamp 8 Replace the bracket and fix the cuvette Next Steps 9 Install the cuvette holder in the instrument 10 Perform your verification 1220 Infinity LC 193 6 Preventive Maintenance and Repair Detector Correcting Leaks When If a leakage has occurred in the flow cell area or at the capillary connections Tools required Tissue Two 1 4 inch wrenches for capillary connections Parts required Description None Remove the lower front cover 2 Use tissue to dry the leak sensor area 3 Observe the capillary connections and the flow cell area for leaks and correct if required 4 Replace the front cover Leak plane Waste outlet Leak sensor assembly 194 1220 Infinity LC Preventive Maintenance and Repair 6 Algae Growth in HPLC Systems 1220 Infinity LC The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instru
5. Agilent Technologies Notices Agilent Technologies Inc 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64280 90010 Edition 07 10 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreemen
6. ece 3 Agilent 1220 Infinity LC Description Agilent 1220 Infinity LC electronics 38 Solvent Delivery System 39 Overview 39 Degasser 39 Principles of Operation 40 Compressibility Compensation 43 Variable Stroke Volume 45 Using the Pump 46 Injection System 48 Manual Injector 48 Autosampler 51 Column Oven 62 Detector 63 This chapter provides general information about the functionality and use of the Agilent 1220 Infinity LC system and its components n Agilent Technologies 37 3 Agilent 1220 Infinity LC Description Agilent 1220 Infinity LC electronics All electrical connectors are placed on the rear left side of the instrument Available connectors 38 Power connector female end LAN connector Agilent 1220 Infinity LC to controlling PC CAN connectors Agilent 1220 Infinity LC to additional Agilent 1200 Series Module USB connector for future use RS232 connector APG Remote connector 12V DC Output 8 bit configuration switch see LAN Configuration on page 22 5 Main Board fuses 250Vac T3 15A 2110 1417 Fuse F1 Degasser Pump Injector Motors Fuse F2 Injector sensors Column Oven Ext 24V Connector Fuse F3 Processor Core 5V 15V 15V supply on Mainboard Fuse F4 VWD incl D2 Lamp Fuse F5 VWD Heater FAN Next to each fuse is a LED Red LED indicates the fuse is blown If one of the fuses is blown the green LED of the power switch flashes Fuse Netfilter 250Vac TIOAH 2110 1004 1220 Infinit
7. r A A ll hi Ji l u J l y A l 20 30 Time min Figure 24 Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards To check photometric accuracy of the Agilent 1220 Infinity LC VWD it is necessary to have more precise information on path lengths of the VWD flow cells The correct response is expected response correction factor Details of the Agilent 1220 Infinity LC VWD flow cells are shown in Table 29 on page 66 1220 Infinity LC 65 3 Agilent 1220 Infinity LC Description Table 29 Correction factors for Agilent VWD flow cells Flow cell type Cell Part number Pathlength Path length Correction volume nominal actual factor Standard flow cell 14 ul G1314 60086 10mm 10 15 0 19 mm 10 10 15 Semi micro flow cell 5 yl G1314 60083 6 mm 6 10 0 19 mm 6 6 10 Micro flow cell 2 ul G1314 60087 3mm 2 80 0 19 mm 3 2 8 High Pressure flow cell 14 ul G1314 60082 10 mm 10 00 0 19 mm 6 5 75 Be aware that there are additional tolerance of gasket thickness and its compression ratio which are considered to be very small in comparison with the machining tolerance 1220 Infinity LC 1220 Infinity LC 4 Test Functions and Calibration Agilent 1220 Infinity LC System 69 Installation Check 69 Module Info 70 State Info 71 Solvent Delivery System 72 LeakTest 71
8. 2 Installation Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the Agilent 1220 Infinity LC is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the Agilent 1220 Infinity LC Table 15 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF manually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full duplex 28 Storing the settings permanently with Bootp If you want to change parameters of the Compact LC using the Bootp follow the instructions below 1 Turn off the Agilent 1220 Infinity LC 2 Change the Configuration Switch settings of the Agilent 1220 Infinity LC to Bootp amp Store mode 3 Start the Agilent Boo
9. 2 Stator head 0101 1417 3 Stator screw 1535 4857 214 1220 Infinity LC Parts for Maintenance and Repair 7 Column Oven Table 56 Column oven part number Description Part Number Complete column oven assembly G4280 60040 Heater door assembly G4280 60017 1220 Infinity LC 215 7 Parts for Maintenance and Repair Detector 216 Standard Flow Cell Table 57 Standard flow cell part numbers Item Description Part Number Standard flow cell 10mm 14 uL 40 bar 1 Cell screw kit quantity 2 2 Conical spring kit quantity 10 3 Ring 1 PEEK kit quantity 2 4 Gasket 1 small hole KAPTON quantity 10 5 Window quartz kit quantity 2 6 Gasket 2 large hole KAPTON quantity 10 7 Ring 2 PEEK kit quantity 2 G1314 60086 G1314 65062 79853 29100 G1314 65065 G1314 65063 79853 68742 G1314 65064 G1314 65066 1220 Infinity LC Parts for Maintenance and Repair 7 Cell screw Conical springs Ring 1 PEEK Gasket 1 small hole Window Quartz Gasket 2 large hole Ring 2 PEEK o oc FrwWn Detector Lamp Table 58 Variable Wavelength Detector lamp Description Part Number Deuterium lamp G1314 60100 1220 Infinity LC 217 7 Parts for Maintenance and Repair Detector Optical Unit and Fan Assembly Table 59 VWD optical unit part number Description Part Number Complete optical unit assembly G1314 60061 Fan
10. Exchange the lamp Please contact your Agilent service representative Every time the deuterium lamp or the tungsten lamp is switched on or off a heater self test is performed If the test fails an error event is created As a result the temperature control is switched off Probable cause 1 Defective connector or cable 2 Defective heater 1220 Infinity LC Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative 129 5 130 Error Information Heater Power At Limit The available power of the heater reached either the upper or lower limit This event is sent only once per run The parameter determines which limit has been hit 0 means upper power limit hit excessive ambient temperature drop 1 means lower power limit hit excessive ambient temperature increase Probable cause Suggested actions 1 Ambient conditions have changed too much Verify that the reproducibility of your results during the run so that optimum results may is not affected not be guaranteed Expose the detector to more stable ambient conditions Holmium Oxide Test Failed The holmium oxide test in the detector has failed During the holmium test the detector moves the holmium filter into the light path and compares the measured absorbance maxima of the holmium oxide filter with expected maxima If the measured maxima are outside the limits the error messag
11. 1220 Infinity LC Test Functions and Calibration Scan Parameters UV Lamp On Blank Scan VWD only Sample Scan Export Data Switches on the UV lamp Scans a blank spectrum solvent only over the specified wavelength range at the specified resolution You specify the wavelength range in the from and to fields and the resolution in the step field Scans the sample spectrum over the specified wavelength range at the specified resolution You specify the wavelength range in the from and to fields and the resolution in the step field Exports the selected data in csv format Using the options in the Export Filter section you can select to export only the peak maxima all peak data the peak maximum and four adjacent values to left and right or all data points 4 93 4 Test Functions and Calibration Detector 94 1220 Infinity LC 1220 Infinity LC 5 Error Information What are Error Messages 98 General Error Messages 99 Compensation Sensor Open Compensation Sensor Short Fan Failed 100 Leak 101 Leak Sensor Open 101 Leak Sensor Short 102 OpenCover 102 Remote Timeout 103 Shut Down 103 Synchronization Lost 104 Timeout 104 Pump Error Messages 105 Encoder Missing 105 Index Adjustment 105 Index Limit 106 Index Missing 106 Initialization Failed 107 Missing Pressure Reading Motor Drive Power 108 Pressure Above Upper Limit Pressure Below Lower Limit Pressure Signal Missing Pump Configuration 110
12. C the oven tries to attain a temperature of 30 C 30 C is used as the start temperature Ifthe current oven temperature is above 30 C but below 50 C the current oven temperature is used as the start temperature Ifthe current oven temperature is above 50 C an error message is displayed The oven must then be allowed to cool to below 50 C before the test can be run Oven Test Evaluation At the end of the Oven Test the slope of the temperature rise of the left and right channels are evaluated The test passes if both slopes are gt 4 C min 82 1220 Infinity LC Test Functions and Calibration 4 Oven Calibration Oven Calibration Description The oven calibration procedure enables the oven temperature to be measured against an external calibrated measuring device Normally temperature calibration is not required during the lifetime of the instrument however in order to comply with local regulatory requirements the 2 point calibration and verification procedure may be performed Running the Oven Calibration For the measurement and calibration procedure we recommend a measuring device that provides the necessary resolution and precision for example Hereaus Quat340 quartz surface temperature measurement sensor Contact your local Agilent Technologies support representative for ordering information Install the calibrated temperature measuring device Select the Oven Calibrationin the user interface Wait fo
13. Gasket 2 large hole Ring 2 PEEK RFID tag Figure 29 Description Standard flow cell 10 mm 14 pL 40 bar Gasket 1 small hole i d 1 mm o d 7 9 mm KAPTON 7A 1 2 9 z UO lay A 3 8 Cp T CLA vom 8 SE v CA A YY 9 6 Standard Flow Cell 1 Press the release buttons and remove the lower front cover to gain access to the flow cell area 2 Disconnect the inlet and outlet capillaries 3 Unscrew the thumb screws in parallel and remove the flow cell 4 Disassembling the Flow Cell a Unscrew the cell screw using a 4 mm hexagonal wrench b Remove the SST rings using a pair of tweezers 1220 Infinity LC Preventive Maintenance and Repair 6 CAUTION Window surfaces scratched by tweezers Window surfaces can easily be scratched by using tweezers to remove the windows gt Do not use tweezers to remove the windows 1220 Infinity LC c Use adhesive tape to remove the PEEK ring the window and the gasket d Repeat step 1 through step 3 for the other window Keep the parts separate otherwise they could get mixed up 5 Cleaning the Flow Cell Parts a Pour isopropanol into the cell hole and wipe clean with a piece of lint free cloth b Clean the windows with ethanol or methanol Dry with a piece of lint free cloth 6 Reassembling the Flow Cell a Hold the flow cell cassette horizontal and place the gasket in position Ensure that both cell holes can be seen through the holes of gaske
14. Pressure Too High Check 72 Purge Pump 73 Autosampler 75 Maintenance Positions 75 Injector Steps 77 Alignment Teaching 78 Gripper Verification 81 Column Oven 82 Oven Test 82 Oven Calibration 83 Detector 84 Cell Test 84 Dark Current Test 85 Holmium Oxide Test 86 Intensity Test 87 Filter Grating Motor Test 89 Detector Calibration 90 Test Chromatogram 91 Spectral Scan 92 XE Agilent Technologies 67 4 Test Functions and Calibration This chapter describes the tests calibrations and tools that are available with the Instrument Utilities software or the Lab Advisor 68 1220 Infinity LC Test Functions and Calibration 4 Agilent 1220 Infinity LC System Installation Check The Installation Check switches on all available modules purges the system for five minutes at 1 mL min tests the flow path by applying a pressure up to 200 bar and switches on the oven if available and detector The pump and detector are mandatory for this check the oven and autosampler are optional The installation check passes if the following conditions are met All modules switch on successfully within the timeout period 120 seconds The pump achieves 200 bar after 5 minutes The oven reaches 2 K above its actual temperature The detector lamp ignites and the detector reaches a Ready state 1220 Infinity LC 69 4 70 Test Functions and Calibration Module Info Module Info Description The Module Info tool collects diagn
15. built in holmium oxide filter to verify wavelength accuracy see also Wavelength Calibration The test evaluates the results automatically and provides a spectrum of the holmium oxide filter To eliminate effects due to absorbing solvents the test should be done with water in the flow cell On completion of the test the results are displayed automatically Holmium Oxide Test Report VWD Holmium Spectrum Absorbance AU 57 f th l 2 14 X IN ide MEER IL ds 0 a ry 200 300 400 500 600 Wavelength nm VWD Holmium Test Results Specification Measured Result Deviation from wavelength 1 360 8 nm s nm 0 0 nm Pas Deviation from wavelength 2 418 5 nm Lewd nm 0 1 nm Pa Deviation from wavelength 3 536 4 nm 1 1 nm 0 0 nm Pa 1220 Infinity LC Test Functions and Calibration 4 Evaluating the VWD Holmium Oxide Test The test is evaluated by the instrument and the measured maxima are displayed automatically The test fails if one or more of the maxima lies outside the limits Table 35 Limits Absorbance Limits Maxima 360 8 nm 1 to 1 nm 418 5 nm 1 to 1 nm 536 4 nm 1 to 1 nm Intensity Test VWD Intensity Test Description The intensity test measures the intensity of the UV lamp over the full VWD wavelength range 190 800 nm The test evaluates the results automatically and provides an intensity spectrum The test evaluates the highest int
16. module components are defective 3 Defective main board Exchange the main board 1220 Infinity LC 109 5 Error Information Pressure Signal Missing The pressure signal from the damper is missing The pressure signal from the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120mV across the damper connector Probable cause Suggested actions 1 Damper disconnected Ensure the damper is connected correctly to the main board 2 Defective damper Exchange the damper Pump Configuration At switch on the gradient pump has recognized a new pump configuration The gradient pump is assigned its configuration at the factory If the gradient valve is disconnected and the gradient pump is rebooted the error message is generated However the pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable cause Suggested actions 1 Gradient valve disconnected Reconnect the gradient valve 110 1220 Infinity LC Error Information 5 Pump Head Missing The pump head end stop in the pump was not found When the pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 seconds indicated by an increase in motor current If the end point is not found within 20 seconds the error message is generated Probable cause Suggested actio
17. 210 W 717 BTU Maximum 0 55 C 32 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Non condensing Up to 2000 m 6500 ft Up to 4600 m 14950 ft For storing the instrument Installation Category Il Pollution Degree 2 For indoor use only All materials recyclable 12 1220 Infinity LC Performance Specifications Introduction 1 Performance Specifications Agilent 1220 Infinity LC Table 2 Performance Specifications Agilent 1220 Infinity LC Type Specification Safety features Control and data evaluation Communications GLP features Extensive diagnostics error detection and display leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Agilent EZChrom Compact Agilent ChemStation Agilent Instrument Utilities Agilent Lab Advisor Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN Early maintenance feedback EMF electronic records of maintenance and errors Performance Specifications Agilent 1220 Infinity LC Pump 1220 Infinity LC Table3 Performance Specifications Agilent 1220 Infinity LC Pump Type Specification Hydraulic system Settable flow range Flow range Dual plunger in series pump with proprietary servo controlled variable stroke drive floating plungers and passive inlet valve 0 001 10 ml min in 0 001 ml min
18. 5 The gradient valve in the quaternary pump has drawn excessive current causing the electronic fuse to open Probable cause 1 Defective gradient valve 2 Defective connection cable front panel to main board 3 Defective main board Suggested actions Restart the quaternary pump If the error message appears again exchange the gradient valve Exchange the connection cable Exchange the LPM board 115 5 116 Error Information Wait Timeout When running certain tests in the diagnostics mode or other special applications the pump must wait for the plungers to reach a specific position or must wait for a certain pressure or flow to be reached Each action or state must be completed within the timeout period otherwise the error message is generated Possible Reasons for a Wait Timeout Pressure not reached Pump channel A did not reach the delivery phase Pump channel B did not reach the delivery phase Pump channel A did not reach the take in phase Pump channel B did not reach the take in phase Solvent volume not delivered within the specified time Probable cause Suggested actions 1 Flow changed after starting test Ensure correct operating condition for the special application in use 2 Defective pump drive assembly Exchange the defective pump drive assembly Zero Solvent Counter Pump firmware version A 02 32 and higher allow to set solvent bottle fillings in the data system
19. 64280 80004 VWD heater G1314 60113 Lamp cable G4280 81607 VWD heater board G1314 65826 VWD temp sensor board G1314 65802 NOTE Repairs to the optical unit require specialist knowledge 218 1220 Infinity LC P 1220 Infinity LC e e e n e oa 7 0 905 698 Qeu9 Upgrading the Agilent 1220 Infinity LC e e Oven Upgrade 220 ES Agilent Technologies 219 8 Upgrading the Agilent 1220 Infinity LC Oven Upgrade Tools required None Parts required Description Oven upgrade kit Switch off the instrument Remove the lower front cover Disconnect the column and remove it Remove the leak tube ao A WN Press the knurled portion at either side of the column tray inwards and remove the column tray Unpack the oven upgrade kit and separate the two parts 7 Click the oven into position in place of the column tray The electrical connection to the oven is made automatically Replace the leak tube Click the oven insulation into place in the lower front cover with the cutout in the oven insulation support at the bottom 10 Replace the column and reconnect the capillaries 11 Replace the lower front cover 12 Open the Instrument Utilities software navigate to the Module Service Center and click Add new oven Follow the instructions to reconfigure your instrument 13 In the instrument control software autoconfigure the instrument to register the change to the instrument configuration 220 1
20. If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probable cause Suggested actions 1 Volume in bottle below specified volume Refill bottles and reset solvent counters 2 Incorrect setting of limit Make sure the limits are set correctly 1220 Infinity LC Autosampler Error Messages Error Information 5 These errors are specific to the autosampler Arm Movement Failed 1220 Infinity LC The transport assembly was unable to complete a movement in one of the axes The processor defines a certain time window for the successful completion of a movement in any particular axis The movement and position of the transport assembly is monitored by the encoders on the stepper motors If the processor does not receive the correct position information from the encoders within the time window the error message is generated See figure Figure 22 on page 57 for axes identification Arm Movement 0 Failed X axis Arm Movement 1 Failed Z axis Arm Movement 2 Failed Theta gripper rotation Arm Movement 3 Failed Gripper gripper fingers open close Probable cause 1 Mechanical obstruction High friction in the transport assembly Defective motor assembly Defective sample transport assembly flex board Defective main board Suggested actions Ensure unobstructed movement of the transport assembly Exchange the sample transport as
21. Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly Exchange the pump drive assembly 4 Defective main board Exchange the main board Temperature Out of Range The temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable cause Suggested actions 1 Defective main board Exchange the main board 1220 Infinity LC 113 5 Error Information Valve Failed Valve 0 Failed valve A Valve 1 Failed valve B Valve 2 Failed valve C Valve 3 Failed valve D One of the valves of the multi channel gradient valve has failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable cause Suggested actions 1 Gradient valve disconnected Ensure the gradient valve is connected correctly 2 Connection cable inside instrument not Ensure the connection cable is connected connected correctly 3 Connection cable inside instrument Exchange the connection cable defective 4 Gradient valve defective Exchange the gradient valve 114 1220 Infinity LC Valve Fuse 1220 Infinity LC Valve Fuse 0 Channels A and B Valve Fuse 1 Channels C and D Error Information
22. VWM board ataregular interval at least once per year for example prior to an Operational Qualification Performance Verification procedure and when chromatographic results indicate the detector may require recalibration Test Chromatogram 1220 Infinity LC A built in pre defined test chromatogram on the VWM board is processed through ADC like normal signals from the diodes and can be used to check the ADC and the data handling system The signal is available at the analog output and on the GPIB The run time of the chromatogram is depending on the setting for response time peak width If no stop time is set the chromatogram will repeat continuously Response Time Stop Time 0 06 sec 0 8 min 0 12 sec 0 8 min 0 25 sec 0 8 min 91 4 Test Functions and Calibration Response Time Stop Time 0 50 sec 0 8 min 1 00 sec 1 6 min 2 00 sec 3 2 min Default settings 4 00 sec 6 4 min 8 00 sec 12 8 min The test chromatogram has four main peaks with the following absorbances Peak Absorbance approx 38 mAU 100 mAU 290 mAU Siw n 20 mAU Spectral Scan The Spectral Scan tool is available for diode array and variable wavelength detectors DAD MWD and VWD It allows you to scan a spectrum over a specified wavelength range and export the data to a csv comma separated values file that can be used in other applications for example Microsoft Excel 92 1220 Infinity LC
23. action Absorbing solvent Ensure the flow or air bubble in cell is filled with flow cell water and free from air bubbles Dirty or Exchange the contaminated flow cell flow cell windows 1220 Infinity LC Test Functions and Calibration 4 Dark Current Test 1220 Infinity LC VWD Dark Current Test Description The dark current test measures the leakage current from the sample and reference circuits The test is used to check for defective sample or reference diodes or ADC circuits which may cause non linearity or excessive baseline noise During the test the lamp is switched off Next the leakage current from both diodes is measured The test evaluates the results automatically Evaluating the VWD Dark Current Test Table32 Limits Sample circuit lt 7900 counts Reference circuit lt 900 counts Probable causes of test failure Table 33 Sample circuit noise exceeds limit Cause Corrective action Defective sample Exchange the diode sample diode Defective sample Exchange the ADC board sample ADC board Table 34 Reference circuit noise exceeds limit Cause Corrective action Defective reference diode Exchange the reference diode Defective Exchange the reference ADC reference ADC board board 85 86 4 Test Functions and Calibration Holmium Oxide Test VWD Holmium Oxide Test Description The holmium oxide test uses three characteristic absorbance maxima of the
24. from the Outlet Ball Valve Remove the waste tubing and disconnect the inlet valve tubing Remove the capillary at the bottom of the Pumphead O a A c N Using a 4 mm hexagonal key loosen the two pumphead screws stepwise and remove the pumphead from the pump drive Pump head screws 1220 Infinity LC 151 6 Preventive Maintenance and Repair Exchanging the Pump Seals and Seal Wear in Procedure Exchanging the Pump Seals When Seal leaking if indicated by the results of the leak test Tools required Wrench 1 4 inch 4 mm hexagonal key Parts required Description 5063 6589 Metering seal pack of 2 for 100 pl analytical head 0905 1420 PE seals pack of 2 5022 2159 Restriction capillary Preparations Switch off the pump at the main power switch Remove the upper front cover 1 Place the pump head on a flat surface Loosen the lock 2 Remove the support rings from the plunger housing and screw two revolutions and while holding the lower half lift the housing away from the plungers of the assembly carefully pull the pump head away from the plunger housing Pump head zi j a Lock screw Support ring Plunger housing Plunger housing l Plunger 152 1220 Infinity LC Preventive Maintenance and Repair Solvent Delivery System 3 Using one of the plungers carefully remove the seal from the pump head be careful not to break the plunger Remove wear retainers if st
25. increments 0 2 10 0 ml min 13 1 14 Introduction Table3 Performance Specifications Agilent 1220 Infinity LC Pump Type Specification Flow precision Flow accuracy Pressure Pressure pulsation Compressibility compensation Recommended pH range Gradient formation optional Composition Range Composition Precision lt 0 07 RSD or lt 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pl min whatever is greater Operating range 0 60 MPa 0 600 bar 0 8820 psi up to 5 ml min Operating range 0 20 MPa 0 200 bar 0 2950 psi up to 10 ml min lt 2 amplitude typically lt 1 96 at 1 ml min isopropanol at all pressures gt 1 MPa 10 bar User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Low pressure dual mixing gradient capability using proprietary high speed proportioning valve Delay volume 800 1100 ul dependent on back pressure 0 95 or 5 100 user selectable lt 0 2 RSD at 0 2 and 1 ml min Performance Specifications Agilent 1220 Infinity LC Autosampler Table4 Performance Specifications Agilent 1220 Infinity LC Autosampler Type Specification Pressure Injection range Operating range 0 60 MPa 0 600 bar 0 8820 psi 0 1 100 ul in 0 1 pl increment
26. leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition 103 Error Information Synchronization Lost During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause 1 CAN cable disconnected 2 Defective CAN cable 3 Defective main board in another module The timeout threshold was exceeded Probable cause 1 Theanalysis was completed successfully and the timeout function switched off the module as requested 2 Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Suggested actions Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly Exchange the CAN cable Switch off the system Restart the system and determine which module or modules are not recognized by the system Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition Restart the anal
27. limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs Threshold Limit Values according to the American Conference of Governmental Industrial Hygienists Table 61 UV Radiation Limits Exposure day Effective Irradiance 8 hours 0 1 uW cm 10 minutes 5 0 W cm Typically the radiation values are much smaller than these limits Table 62 UV Radiation Typical Values Position Effective Irradiance Lamp installed 50 cm distance average 0 016 pW cm2 Lamp installed 50 cm distance maximum 0 14 pW cm2 1220 Infinity LC Appendix 9 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure L lt 70 dB A AtOperator Position Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test 1220 Infinity LC 229 9 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 230 Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requir
28. of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1220 Infinity LC In This Book 1220 Infinity LC In This Book This manual contains information on how to use maintain repair and upgrade the Agilent 1220 Infinity LC System Introduction This chapter provides an overview of the Agilent 1220 Infinity LC available configurations site requirements and specifications Installation This chapter provides an overview on shipment content and installation Agilent 1220 Infinity LC Description This chapter provides general information about the functionality and use of the Agilent 1220 Infinity LC system and its components Test Functions and Calibration This chapter describes the tests calibrations and tools that are available with the Instrument Utilities software or the Lab Advisor Error Information This chapter provides information on the error messages that might be displayed and gives the possible causes and suggestions on their solutions Preventive Maintenance and Repair Preventive Maintenance PM is an Agilent Technologies recommended proce
29. only Safety flap flex board It is strongly recommended that the exchange of the safety flap and flex board is done by Agilent trained service personnel Transport assembly parts The adjustment of the motors and the tension on the drive belts are important for correct operation of the transport assembly It is strongly recommended that exchange of drive belts and the gripper assembly is done by Agilent trained service personnel There are no other field replaceable parts in the transport assembly If any other component is defective flex board spindles plastic parts the complete unit must be exchanged Cleaning the autosampler Electrical shock hazard Liquid dripping into the autosampler could cause shock hazard and damage to the autosampler gt Drain all solvent lines before opening any fittings 1220 Infinity LC The autosampler covers should be kept clean Clean with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth that liquid can drip into the autosampler Maintenance Functions Certain maintenance procedures require the needle arm metering device and gripper assembly to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position 165 6 Preventive Maintenance and Repair Exchanging the Needle Assembly When Tools required Part
30. safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1220 Infinity LC 223 9 Appendix Safety Symbols Table 60 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the N instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Z Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced Z by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up EA UMS alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions
31. the Agilent 1120 Infinity LC pump it is recommended to connect the salt solution to one of the lower gradient valve ports and the organic solvent to one of the upper gradient valve port It is best to have the organic channel directly above the salt solution channel Regular flushing with water of all DCGV channels is recommended to remove all possible salt deposits in the valve ports Before operating the pump flush the vacuum degasser optional with at least two volumes 3 ml especially when the pump has been turned off for some time for example overnight and volatile solvent mixtures are used in the channels Prevent blocking of solvent inlet filters never use the pump without solvent inlet filter Growth of algae should be avoided Check the purge valve frit and column frit regularly A blocked purge valve frit can be identified by black or yellow layers on its surface or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 ml min with an open purge valve When using the pump at low flow rates for example 0 2 ml min check all 1 16 inch fittings for any signs of leaks When exchanging the pump seals also exchange the purge valve frit When using buffer solutions flush the system with water before switching it off Check the pump plungers for scratches when changing the plunger seals Scratched plungers will lead to micro leaks and will decrease the lifetime of the seal After changing the p
32. to ensure correct operation The metering device is always flushed after injection to ensure minimum carry over The six port injection valve unit only 5 ports are used is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosampler and directly connects the flow from the pump to the column During injection and analysis the valve unit directs the flow through the autosamplers which ensures that the sample is injected completely into the column and that any sample residue is removed from the metering unit and needle from before the next sampling sequence begins Sampling Sequence The movements of the autosampler components during the sampling sequence are monitored continuously by the processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence can t be completed successfully an error message is generated Solvent is bypassed from the autosamplers by the injection valve during the sampling sequence The sample vial is selected by a gripper arm from a static sample rack The gripper arm places the sample vial below the injection needle The required volume of sample is drawn into the sample loop by the metering device Sample is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The in
33. valve unit at port 1 and flows directly to the column through port 6 Figure 19 Bypass Position 53 3 Agilent 1220 Infinity LC Description Injection System Next the needle is raised and the vial is positioned below the needle The needle moves down into the vial and the metering unit draws the sample into the sample loop Figure 20 Drawing the Sample When the metering unit has drawn the required volume of sample into the sample loop the needle is raised and the vial is replaced in the sample tray The needle is lowered into the needle seat and the injection valve switches back to the mainpass position flushing the sample onto the column 54 1220 Infinity LC 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Figure 21 Mainpass Position Sample Injection Sampling Unit The sampling unit comprises three main assemblies needle drive metering device and injection valve The replacement sampling unit excludes the injection valve and metering head assemblies Needle Drive The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly The upper and lower needle positions are detected by reflection sensors on the sampling unit flex board while the needle in vial position is determined by counting the motor steps from the upper needle sensor position An
34. 1 Syringe plastic 9301 0411 1 Syringe adapter 9301 1337 1 Screw cap vial clear 6 mL 9301 1377 1 pack of 100 Screw caps for 6 mL vials pack 9301 1379 1 of 100 Solvent reservoir 1 L 9301 1420 1 Bottle head assembly G1311 60003 1 Accessory Kit Contents for G4288B Table9 Accessory Kit Contents for G4288B Description Part Number Quantity Accessory kit complete G4288 68755 Fitting onepiece fingertight 0100 2562 1 PTFE tubing 0 052 ID 0890 1195 5m Flexible tubing to waste 0890 1711 3m 1220 Infinity LC 19 2 20 Installation Table9 Accessory Kit Contents for G4288B Description Part Number Quantity Crossover patch cable 5023 0203 1 Waste accessory kit 5062 8535 1 PTFE silicon septa 16 mm 5188 2758 1 pre slit pack of 100 Syringe 50 pL 5190 1501 1 Syringe plastic 9301 0411 1 Syringe adapter 9301 1337 1 Screw cap vial clear 6 mL 9301 1377 1 pack of 100 Screw caps for 6 mL vials pack 9301 1379 1 of 100 Solvent reservoir 1 L 9301 1420 1 Solvent reservoir amber 1 L 9301 1450 1 Bottle head assembly G1311 60003 2 Accessory Kit Contents for G4290B Table 10 Accessory Kit Contents for G4290B Description Part Number Quantity Accessory kit complete 64290 68755 Fitting onepiece fingertight 0100 2562 1 PTFE tubing 0 052 ID 0890 1195 5m Flexible tubing to waste 0890 1711 3m Crossover patch cable 5023 0203 1 Waste accessory kit 5062 8535 1 Syringe plastic 9301 0411 1 S
35. 220 Infinity LC 1220 Infinity LC 9 Appendix General Safety Information 222 Solvent Information 225 Radio Interference 227 UV Radiation 228 Sound Emission 229 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 230 Declaration of Conformity for HOX2 Filter 231 Agilent Technologies on Internet 232 RE Agilent Technologies 221 9 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired The operator of this instrument is advised to use the equipment in a manner as specified in this manual 222 Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switche
36. 25260 6 Passive inlet valve G1312 60066 7 Outlet ball valve G1312 60067 8 Screw lock 5042 1303 9 Purge valve assembly G4280 60061 10 Screw M5 60 mm 0505 2118 1220 Infinity LC Parts for Maintenance and Repair 7 Solvent Delivery System 1220 Infinity LC 201 7 Parts for Maintenance and Repair Outlet Ball Valve Assembly Table 45 Outlet Ball Valve part numbers Item Description Part Number Complete outlet ball valve G1312 60067 assembly 202 1220 Infinity LC Parts for Maintenance and Repair 7 Purge Valve Assembly Table 46 Purge Valve part numbers Item Description Part Number Complete purge valve 64280 60061 assembly 1 Valve body no part number 2 PTFE frit pack of 5 01018 22707 3 Gold seal 5001 3707 4 Cap pack of 4 5062 2485 1220 Infinity LC 203 7 Parts for Maintenance and Repair Passive Inlet Valve Assembly Table 47 Passive Inlet Valve part numbers Item Description Part Number Complete passive inlet valve G1312 60066 assembly 204 1220 Infinity LC Bottle Head Assembly 1220 Infinity LC Parts for Maintenance and Repair Table 48 Bottle head part numbers 7 Item Description Part Number Complete bottle head assembly G1311 60003 1 Ferrules with lock ring pack of 5063 6598 10 2 Tube screw pack of 10 5063 6599 3 Wire marker No part number 4 Solvent tubing 5 m 5062 2483 5 Frit adapter pack of 4 5062 8517 6 Solvent inlet filter 20 um
37. 50 Blue polypropylene Clear PTFE red rubber 5182 3458 Green polypropylene Clear PTFE red rubber 5182 3457 Red polypropylene Clear PTFE red rubber 5182 3459 Table 28 Screw Caps Description Septa 100 Pack Blue polypropylene Clear PTFE red rubber 5182 0717 Green polypropylene Clear PTFE red rubber 5182 0718 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Table 28 Screw Caps Description Septa 100 Pack Red polypropylene Clear PTFE red rubber 5182 0719 Blue polypropylene Clear PTFE silicone 5182 0720 Green polypropylene Clear PTFE silicone 5182 0721 Red polypropylene Clear PTFE silicone 5182 0722 1220 Infinity LC 61 3 Agilent 1220 Infinity LC Description Column Oven 62 The column oven is based on a resistor heater matt with two thermal sensors to provide constant temperature in the whole column area A build in over temperature cut off fuse inhibits overheating The inner volume of the oven capillary is 6pl Maximum column length is 25cm 10 inch Operational range is 5 degree above ambient at least 10 C up to 60 C max specified flow rate is 5ml min at 60 C Never operate the column oven with open front cover to ensure a correct column temperature always operate with closed front cover The counterpart of the oven isolation is fixed at the inner side of the front cover 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Detector Detector The Agilent 1220 Infinity LC vari
38. 5041 2168 205 7 206 Parts for Maintenance and Repair Hydraulic Path Table 49 Hydraulic path part numbers Item Description Part Number 1 Outlet capillary pump to G1312 67305 injection device Bottle head assembly bottleto G7311 60003 passive inlet valve or vacuum degasser 2 Solvent tube vacuum degasser G4280 60034 to DCGV 3 Capillary plunger 1 to damper G4280 81300 4 Capillary damper to plunger2 G4280 81301 5 Connecting tube DCGV to 5067 4693 passive inlet valve 6 Waste tube reorder pack 5m 5062 2461 Not visible Damper 600 bar G1311 60065 Not visible Pump drive G1311 60001 Not visible DCGV G4280 60004 Not visible Fan 3160 1017 1220 Infinity LC Parts for Maintenance and Repair 7 Solvent Delivery System 1220 Infinity LC 207 7 Parts for Maintenance and Repair Injection System Manual Injector Injection Valve Assembly Table 50 Manual Injection Valve part numbers Item Description Part Number Complete manual injection 5067 4102 valve assembly including 20 pL loop capillary and needle port Manual injection valve 5067 4202 excluding loop capillary and needle port 1 Bearing ring 1535 4045 2 Rotor seal PEEK 0101 1409 3 Stator head 0101 1417 4 Needle port 5067 1581 5 Stator screw 1535 4857 Hex key 9 64 inch for stator 8710 0060 screws not shown 208 1220 Infinity LC 1220 Infinity LC Sample Loops Table 51 Sample loops for manual i
39. 6 Preventive Maintenance and Repair Exchanging the Dual Channel Gradient Valve DCGV Tools required Parts required Preparations Screwdriver Pozidriv 1 p n Description 1 G4280 60004 Dual channel gradient valve Switch off the pump at the power switch Remove the upper front cover to gain access to the pump mechanics The lifetime of the dual channel gradient valve can be maintained by regularly flushing the valve especially when using buffer solutions If using buffer solutions flush all channels of the valve with water to prevent precipitation of the buffer Salt crystals can be forced into an unused channel and form plugs that may lead to leaks of that channel such leaks will interfere with the general performance of the valve When using buffer solutions and organic solvents in the Agilent 1220 Infinity LC Pump it is recommended to connect the buffer solution to the lower port of the gradient valve and the organic solvent to the upper port It is best to have the organic channel directly above the salt solution channel A salt solution B organic solvent Solvent tubes 1 Disconnect the connecting tube waste tube and the 2 Pressthe lower sides of the cover to unclip it Remove the solvent tubes from the DCGV unclip them from the tube COVer clips and place them into the solvent cabinet to avoid flow by hydrostatic pressure DCGV Connecting tube DCGV to PIV
40. Exchange the metering plunger and seal Exchange the metering drive motor Exchange the ASM board 119 5 Error Information Missing Vial No vial was found in the position defined in the method or sequence When the gripper arm picks a vial out of the sample tray the processor monitors the gripper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable cause Suggested actions 1 Novial in the position defined in the method Install the sample vial in the correct position or or sequence edit the method or sequence accordingly 2 Incorrect gripper alignment Align gripper 3 Defective gripper assembly defective Exchange the gripper assembly gripper fingers or belt 4 Defective transport assembly flex board Exchange the transport assembly Missing Wash Vial The wash vial programmed in the method was not found When the gripper arm picks a vial out of the sample tray the processor monitors the gripper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable cause Suggested actions 1 No wash vial in the positi
41. Failed The needle arm failed to move down into the needle seat The lower position of the needle arm is monitored by a position sensor on the sampling unit flex board The sensor detects the successful completion of the needle movement to the needle seat position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated Probable cause Suggested actions 1 Needle installed incorrectly or wrong needle Ensure the correct needle type is used and type too long installed correctly 2 Defective or dirty position sensor Exchange the sampling unit flex board 3 Defective motor Exchange the needle drive motor 4 Sticking spindle assembly Exchange the spindle assembly or sampling unit assembly 5 Defective ASM board Exchange the ASM board 122 1220 Infinity LC Error Information 5 Needle Up Failed The needle arm failed to move successfully from the seat or out of the vial to the upper position The upper position of the needle arm is monitored by a position sensor on the sampling unit flex board The sensor detects the successful completion of the needle movement to the upper position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated Probable cause Suggested actions 1 Defective or dirty position sensor Exchange the sampling unit flex board 2 Defective motor Exchan
42. Pump Head Missing 111 Restart Without Cover Servo Restart Failed 112 110 111 99 100 107 109 109 3E Agilent Technologies 95 5 96 Error Information Stroke Length 112 Temperature Limit Exceeded 113 Temperature Out of Range 113 Valve Failed 114 Valve Fuse 115 Wait Timeout 116 Zero Solvent Counter 116 Autosampler Error Messages 117 Arm Movement Failed 117 Initialization Failed 118 Initialization with Vial 118 Invalid Vial Position 119 Metering Home Failed 119 Missing Vial 120 Missing Wash Vial 120 Motor Temperature 121 Needle Down Failed 122 Needle Up Failed 123 Safety Flap Missing 123 Valve to Bypass Failed 124 Valve to Mainpass Failed 124 Vial in Gripper 125 Detector Error Messages 126 ADC Hardware Error 126 Calibration Failed 126 Filter Check Failed 127 Filter Missing 127 Grating Filter Motor Test Failed 128 Grating Missing 128 Heater Current Missing 129 Heater Failed 129 Heater Power At Limit 130 Holmium Oxide Test Failed 130 Illegal Value From Air Inlet Temperature Sensor 131 Illegal Value From Temperature Sensor 131 1220 Infinity LC Error Information 5 Lamp Current Missing 132 Lamp Ignition Failed 132 Lamp Voltage Missing 133 Wavelength Check Failed 133 This chapter provides information on the error messages that might be displayed and gives the possible causes and suggestions on their solutions 1220 Infinity LC 97 5 Error Information What are Er
43. Suggested actions 1 Blocked passive inlet valve Exchange the inlet valve 2 Defective pump drive assembly Exchange the pump drive assembly 3 Defective main board Exchange the main board Missing Pressure Reading The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure readings from the damper every 1ms If the readings are missing for longer than 10 seconds the error message is generated Probable cause Suggested actions 1 Damper disconnected Ensure the damper is connected clean and seated correctly 2 Defective damper Exchange the damper 3 Defective main board Exchange the main board 1220 Infinity LC 107 5 Error Information Motor Drive Power The current drawn by the pump motor exceeded the maximum limit Blockages in the flow path are usually detected by the pressure sensor in the damper which result in the pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper the pressure increase cannot be detected by the pressure sensor and the module will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the module is switched off and the error message is generated Probable cause Suggested actions 1 Flow path blockage in front of the damper Ensure the capillaries and frits between the pump head and damper inlet are free from blockage 2 Blocked out
44. Too High Check Pressure Too High Check Description This test checks the flow path for a blockage and tries to identify the module that is causing the blockage If the blockage is in the autosampler the test can identify whether the blockage occurs in the needle or needle seat The pump and autosampler are necessary to run the Pressure Too High Check Pressure Too High Check Evaluation Start Conditions The pump and autosampler are brought to the READY state and an operating pressure of 200 bar is applied to the system The pump ripple is measured and the start of the test is delayed until the ripple is within the defined limits typically 1 of operating pressure Test Part 1 Part 1 of the test tries to determine in which part of the system the pressure problem lies After the system has achieved the start conditions the autosampler valve is switched from mainpass to bypass and the pressure slice is tested against a limit If the limit is exceeded the pressure problem lies in the autosampler otherwise it lies somewhere in the rest of the flow path Test Part 2 In Part 2 of the test an empty vial is driven to the needle and the valve is switched from bypass to mainpass so that the pressure should drop dramatically The pressure drop is checked against a limit 1220 Infinity LC Test Functions and Calibration 4 If the autosampler is identified as the source of the problem and the limit is not reached the proble
45. able wavelength detector is designed for highest optical performance GLP compliance and easy maintenance with Deuterium lamp for highest intensity and lowest detection limit over a wavelength range of 190 to 600 nm Optional flow cell cartridges standard 10 mm 14 ul high pressure 10 mm 14 ul micro 3 mm 2 ul semi micro 6 mm 5 ul are available and can be used depending on the application needs Easy front access to lamp and flow cell for fast replacement and Built in holmium oxide filter for fast wavelength accuracy verification Match the Flow Cell to the Column Figure 23 on page 64 shows recommendations for flow cells that match the column used If more than one selection is appropriate use the larger flow cell to get the best detection limit Use the smaller flow cell for best peak resolution 1220 Infinity LC 63 3 Agilent 1220 Infinity LC Description Detector Column length lt 5cm 10cm 20 cm gt 40 cm 64 Internal column diameter Recommended flow cell Typical peak width 0 025 min 0 05 min 0 1 min Standard flow cell 0 2 min 1 5 ml min Typical flow 0 2 ml min 0 2 0 4 ml min 0 4 0 8 ml min rate Figure 23 Choosing a Flow Cell Flow Cell Path Length Lambert Beer s law shows a linear relationship between the flow cell path length and absorbance Absorbance log T log 2g C d where is the transmis
46. adio Interference 227 UV Radiation 228 Sound Emission 229 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 230 Declaration of Conformity for HOX2 Filter 231 Agilent Technologies on Internet 232 Contents 6 1220 Infinity LC 1220 Infinity LC 1 Introduction Agilent 1220 Infinity LC Configurations 8 Site Requirements 9 Power Considerations 9 Power Cord 10 Bench Space 10 Environment 11 Physical Specifications 12 Performance Specifications 13 This chapter provides an overview of the Agilent 1220 Infinity LC available configurations site requirements and specifications RE Agilent Technologies 1 Introduction Agilent 1220 Infinity LC Configurations Available configurations of Agilent 1220 Infinity LC The Agilent 1220 Infinity LC is available in three different configurations Possible components include isocratic pump dual channel gradient pump with degasser manual injector autosampler column oven and detector Each configuration comes with at least one pump one injection system and one detector and includes Agilent Instrument Utilities Software Gradient pump Autosampler Column oven Variable Variable Variable wavelength wavelength wavelength detector detector detector G4286B G4288B G4290B A Solvent Selection Valve SSV Upgrade Kit G4280 68708 is available 8 1220 Infinity LC Introduction 1 Site Requirements A suitable environment is important to ensu
47. alization positions of the moving components are sensed by reflection sensors mounted on the flex board These positions are used by the processor to calculate the actual motor position An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly Using the Autosampler Supported trays for the autosampler Table 22 Supported trays for the Autosampler Description Part Number Tray for 100 x 2 ml vials G1313 44510 Halftray for 15 x 6 ml vials G1313 44513 Halftray for 40 x 2 ml vials G1313 44512 Half tray combinations Half trays can be installed in any combination enabling both 2 ml and 6 ml vials to be used simultaneously Numbering of vial positions The standard 100 vial tray has vial positions 1 to 100 However when using two half trays the numbering convention is slightly different The vial positions of the right hand half tray begin at position 101 as follows Left hand 40 position tray 1 40 Left hand 15 position tray 1 15 Right hand 40 position tray 101 140 Right hand 15 position tray 101 115 1220 Infinity LC 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Choice of Vials and Caps For reliable operation vials used with the Agilent 1220 Infinity LC autosampler must not have tapered shoulders or caps that are wider than the body of the vial The vials and caps shown with their part numbers in the tables below have been successfully tested usin
48. alytical head The analytical head is driven by the stepper motor connected to the drive shaft by a toothed belt The drive nut on the spindle converts the circular movement of the spindle to linear motion The drive nut pushes the sapphire plunger against the tension of the spring into the analytical head The base of the 55 3 56 Agilent 1220 Infinity LC Description plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides the movement of the plunger in the analytical head The home position of the plunger is sensed by an infra red sensor on the sampling unit flex board while the sample volume is determined by counting the number of steps from the home position The backward movement of the plunger driven by the spring draws sample from the vial Table 20 Analytical Head Technical Data Standard 100 pl Number of steps 15000 Volume resolution 7 nl motor step Maximum stroke 100 ul Pressure limit 600 bar Plunger material Sapphire Injection Valve The two position 6 port injection valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass end positions No valve adjustments are required after replacing internal components Table21 Injection Valve Technical Da
49. ance and Repair Agilent 1220 Infinity LC System 198 Table 43 System part numbers Description Part Number Power supply 0950 4997 Agilent 1220 Infinity LC main board G4280 65050 Cabinet kit G4280 68713 Front door top G4280 60102 Front door bottom G4280 60001 PSS board Power switch board G4280 65001 FSL board Status LED board G4280 65802 Connecting tube 5067 4693 SSV G4280 68708 Fan ALS G4280 80004 Power switch cable 8121 1833 Cable status LED G4280 81602 Cable temp sensor G4280 81620 Light pipe status G4280 40007 Power switch coupler G4280 40016 Leak plane man inj G4280 44013 Leak panel bottom G4280 44500 Leak plane pump G4280 44501 Leak plane ALS G4280 44502 Holder temp sensor G4280 44016 Leak sensor 5061 3356 1220 Infinity LC Parts for Maintenance and Repair 7 Table 43 System part numbers Description Part Number Main board fuses 3 15AT 2110 1417 Netfilter fuses 10AT 2110 1004 1220 Infinity LC 199 7 Solvent Delivery System 200 Parts for Maintenance and Repair Pump Head Assembly Table 44 Pump Head part numbers Item Description Part Number Complete pump head G1312 60064 assembly includes items marked with 1 Sapphire plunger 5067 4695 2 Plunger housing including G1312 60062 spring 3 Support ring 5001 3739 Backup ring G4220 24103 4 Seal pack of 2 or 5063 6589 Seal pack of 2 for 0905 1420 normal phase applications 5 Pump chamber housing G1312
50. and press Enter to exit with storing parameters c c WINDOWS system32 cmd exe Agilent Technologies G429860 DEGB88080800880 exit Connection to host lost Gi gt Figure 12 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 134 40 27 99 Automatic configuration with Bootp When automatic configuration with Bootp is selected and the LAN interface is powered on it broadcasts a BOOTP Bootstrap Protocol request that contains its MAC hardware address A BOOTP server daemon searches its database for a matching MAC address and if successful sends the corresponding configuration parameters to the compact LC as a BOOTP reply These parameters become the active TCP IP parameters immediately and the TCP IP connection can be established Configuring the Agilent Bootp service program The examples shown in this chapter will not work in your environment You need your own IP address Subnet Mask and Gateway address Ensure that the Agilent 1220 Infinity LC configuration switch is set to either Bootp or Bootp amp Store 33 2 34 Installation Ensure that your instrument is powered off If the Agilent Bootp Service program is not already installed on your PC install it from the folder Bootp on your software CD ROM The Agilent Bootp Service is placed in the
51. bers Item Description Part Number 6 Head body 01078 27710 Screw M5 60 mm long for 0515 2118 mounting assembly not shown 211 7 212 Parts for Maintenance and Repair Sampling Unit Assembly Table 54 Autosampler sampling unit part numbers Item Description Part Number Sampling unit assembly 64280 60027 excluding injection valve and analytical head 1 Sampling unit connector board G1313 66503 SUD 2 Belt gear for metering unit and 7500 0697 needle arm 3 Stepper motor for metering 5062 8590 unit and needle arm 4 Loop capillary 100 uL 01078 87302 5 Analytical head assembly 100 01078 60003 pL 6 Capillary injection valve to 61313 87301 analytical head 160 mm x 0 25 mm 7 Safety cover 61329 44115 8 Injection valve assembly 0101 1422 9 Waste tube injection valve 61313 87300 120 mm 11 Needle seat assembly 0 17 61313 87101 mm ID 2 3 pL 12 Seat adapter 61313 43204 13 Safety flap G1313 44106 14 Flex board G1313 68715 15 Needle assembly for G1313 87201 G 1313 87101 or G1313 87103 needle seat Clamp kit including needle G1313 68713 clamp and two clamp screws 1220 Infinity LC 213 1220 Infinity LC 7 Parts for Maintenance and Repair Injection System Injection Valve Assembly Table 55 Injection valve part numbers Item Description Part Number Complete injection valve 0101 1422 assembly Isolation seal 0100 1852 1 Rotor seal PEEK 0101 1416
52. board VWM board Detector Calibration Wavelength Verification Calibration Wavelength calibration of the detector is done using the zero order position and 656 nm alpha emission line and beta emission line at 486 nm emission line positions of the deuterium lamp The calibration procedure involves three steps First the grating is calibrated on the zero order position The stepper motor step position where the zero order maximum is detected is stored in the detector Next the grating is calibrated against the deuterium emission line at 656 nm and the motor position at which the maximum occurs is stored in the detector Finally the grating is calibrated against the deuterium emission line at 486 nm and the motor position at which the maximum occurs is stored in the detector 1220 Infinity LC Test Functions and Calibration 4 The wavelength verification calibration takes about 2 5 minutes and is disabled within the first 10 minutes after ignition of the lamp because initial drift may distort the measurement When the lamp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically When to Calibrate the Detector The detector is calibrated at the factory and under normal operating conditions should not require recalibration However it is advisable to recalibrate after maintenance flow cell or lamp after repair of components in the optical unit after exchange of the optical unit or
53. ce is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Fan Failed The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 100 1220 Infinity LC Error Information 5 Leak A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries Leak Sensor Open The leak sensor in the module has failed open circuit The curre
54. ch 4 mm hexagonal key Parts required Description 5067 4695 Plunger Preparations Switch off the pump at the main power switch Remove the upper front cover 1 Place the pump head on a flat surface Loosen the lock 2 Remove the support rings from the plunger housing and screw two revolutions and while holding the lower half lift the housing away from the plungers of the assembly carefully pull the pump head away from the plunger housing Pump head A f e SC 0 NS CO QB O Support ring 2 Plunger housing f ms 53 p d m Plunger housing i p dd Plunger 1220 Infinity LC 155 6 Preventive Maintenance and Repair 3 Check the plunger surface and remove any deposits The plunger surface can be cleaned with either alcohol or tooth paste If the plunger is scratched replace it Piston surface 4 Reassemble the pump head assembly Pump head Support ring Plunger housing 156 1220 Infinity LC Preventive Maintenance and Repair 6 Solvent Delivery System Reinstalling the Pump Head Assembly When When reassembling the pump Tools required 4 mm hexagonal key 1 Slide the Pumphead Assembly onto the Pumpdrive 2 Using a 4 mm hexagonal key tighten the Pumphead screws stepwise with increasing torque Pump head screws 3 Reconnect the capillaries and tubing 4 Reinstall the front cover 1220 Infinity LC 157
55. d 1220 Infinity LC Test Functions and Calibration 4 Gripper Verification ALS Gripper Verification Description The verification procedure uses several vial positions as reference points to verify the gripper alignment is correct If verification indicates one or more positions are out of alignment the alignment procedure should be done Verifying the Gripper Positions Vial positions 1 10 55 81 and 100 can be used for position verification 1 Insert empty capped vials into the vial tray at the positions to be verified 2 Select the first vial position in the vial position menu 3 Select Go to selected position 4 If the fingers of the gripper arm are aligned centrally above the vial select the Pick vial button to verify the gripper arm lifts the vial out of the tray correctly If there is a misalignment the gripper must be realigned 5 Select the Put vial button to verify the gripper replaces the vial correctly If there is a misalignment the gripper must be realigned 6 Repeat the procedure for the next vial position 1220 Infinity LC 81 4 Test Functions and Calibration Column Oven Oven Test Oven Test Description The Oven Test is used to evaluate the heating performance of the two Peltier elements The heating rate over a 10 K interval from the start temperature is determined The start temperature must be between 30 C and 50 C and is determined as follows Ifthe current oven temperature is below 30
56. d on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired 1220 Infinity LC Appendix 9 the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite
57. displaced by the first plunger and the remaining half volume is directly delivered into the system During the drawing stroke of the first plunger the second plunger delivers the drawn volume into the system For solvent compositions from the solvent bottles A and B the controller divides the length of the intake stroke into certain fractions in which the gradient valve connects the specified solvent channel to the pump input Table 17 Isocratic pump details Dead volume 800 1100 uL depending on back pressure Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active inlet valve SST gold sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby Adapter SST gold Purge valve SST gold PTFE ceramic PEEK Degasserchamber TFE PDD Copolymer FEP PEEK PPS Table 18 Gradient Pump Details Delay volume 800 1100 uL dependent on back pressure Materials in contact with mobile phase MCGV PTFE Pump head SST gold sapphire ceramic 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Table 18 Gradient Pump Details Active inlet valve SST gold sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby Adapter SST gold Purge valve SST gold PTFE ceramic PEEK Damping unit Gold SST Degasserchamber TFE PDD Copolymer FEP PEEK PPS Compressibility Compensation 1220 Infinity LC Principles of compressibility compensation The compressibil
58. due to hydrostatic pressure 3 Using the 14 mm wrench unscrew the purge valve and remove it 4 Remove the plastic cap with the gold seal from the purge valve 1220 Infinity LC Preventive Maintenance and Repair 6 Solvent Delivery System 5 Using a pair of tweezers or a toothpick remove the frit Valve body dG Figure 27 Purge Valve Parts 6 Place a new frit into the purge valve with the orientation of the frit as shown above 7 Reinstall the cap with the gold seal NOTE If the gold seal is deformed exchange it before reinstalling 8 Insert the purge valve into the pump head and locate the pump outlet capillary and the waste tube 1220 Infinity LC 149 6 Preventive Maintenance and Repair Solvent Delivery System 9 Tighten the purge valve and reconnect outlet capillary and waste tubing Outlet capillary Purge valve Waste tube Figure 28 Exchanging the Purge Valve 150 1220 Infinity LC Preventive Maintenance and Repair 6 Removing the Pump Head Assembly When Before exchanging the seals Before exchanging the plungers Tools required Wrench 1 4 inch 4 mm hexagonal key Preparations Switch off pump at the main power switch WARNING Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed Remove the upper front cover Using a 1 4 inch wrench remove the outlet capillary Disconnect the capillary
59. dure designed to reduce the likelihood of electro mechanical failures Failure to perform preventive maintenance may reduce the long term reliability of your Agilent 1220 Infinity LC Contents Contents Introduction 7 Agilent 1220 Infinity LC Configurations 8 Site Requirements 9 Physical Specifications 12 Performance Specifications 13 Installation 17 Unpacking Your System 18 LAN Configuration 22 Agilent 1220 Infinity LC Description 37 Agilent 1220 Infinity LC electronics 38 Solvent Delivery System 39 Injection System 48 Column Oven 62 Detector 63 Test Functions and Calibration 67 Agilent 1220 Infinity LC System 69 Solvent Delivery System 72 Autosampler 75 Column Oven 82 Detector 84 Error Information 95 What are Error Messages 98 General Error Messages 99 Pump Error Messages 105 Autosampler Error Messages 117 Detector Error Messages 126 1220 Infinity LC 1220 Infinity LC Contents 6 Preventive Maintenance and Repair 135 PM Scope of Work and Checklist 137 Early Maintenance Feedback 138 Solvent Delivery System 141 Manual Injector 160 Autosampler 164 Detector 184 Algae Growth in HPLC Systems 195 Parts for Maintenance and Repair 197 Agilent 1220 Infinity LC System 198 Solvent Delivery System 200 Injection System 208 Column Oven 215 Detector 216 Upgrading the Agilent 1220 Infinity LC 219 Oven Upgrade 220 9 Appendix 221 General Safety Information 222 Solvent Information 225 R
60. e Switch off pump at the main power switch Remove the upper front cover 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet ball valve 2 Using the 14 mm wrench loosen the valve and remove it from the pump body 3 Check that the new valve is assembled correctly and that the gold seal is present if the gold seal is deformed it should be replaced Figure 26 Outlet Ball Valve 1 Outlet ball valve complete assembly G1312 60067 4 Reinstall the outlet ball valve and tighten the valve 1220 Infinity LC Preventive Maintenance and Repair 6 Solvent Delivery System 5 Reconnect the valve capillary Valve capillary Outlet ball valve e 1220 Infinity LC 147 6 Preventive Maintenance and Repair Exchanging the Purge Valve Frit or the Purge Valve When Tools required Parts required Preparations 148 Frit when plunger seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 5 ml min of H30 with purge valve opened Purge valve if internally leaking Wrench 1 4 inch Wrench 14 mm Pairof tweezers or toothpick p n Description 5 01018 22707 PTFE frit pack of 5 1 6G4280 60061 Purge valve Switch off pump at the main power switch Remove the upper front cover 1 Using a 1 4 inch wrench disconnect the pump outlet capillary at the purge valve 2 Disconnect the waste tube Beware of leaking solvents
61. e causes of test failure Cause Corrective action Lamp off Switch on the lamp Old lamp Exchange the lamp Absorbing solvent Ensure the flow or air bubble in cell is filled with flow cell water and free from air bubbles Dirty or Run theCell Test contaminated If the test fails flow cell exchange the flow cell windows VWD Cell Test Description on page 84 Filter Grating Motor Test 1220 Infinity LC VWD Filter Grating Test Description The actual position of the filter motor and grating motor is defined as the number of steps from the reference sensor positions The filter grating test counts the number of motor steps required to move the filter motor and grating motor back to the reference sensor position If the number of steps required to reach the reference positions are the same as the expected step number the test is passed If a motor fails to move or loses motor steps the test fails The test evaluates the results automatically VWD Filter Grating Test Results Probable causes of test failure 89 4 90 Test Functions and Calibration Table 38 Filter Motor Test Cause Corrective action Defective filter Exchange the motor assembly filter motor assembly Defective VWM Exchange the board VWM board Table 39 Grating Motor Test Cause Corrective action Defective filter Exchange the motor assembly filter motor assembly Defective VWM Exchange the
62. e is generated Probable cause Suggested actions 1 Misaligned defective grating assembly Ensure the flow cell is inserted correctly and is free from contamination cell windows buffers and so on Run the filter motor test to determine if the grating motor assembly is defective Exchange the filter motor assembly Run the grating motor test to determine if the grating assembly is defective If defective replace the optical unit 1220 Infinity LC Error Information 5 Illegal Value From Air Inlet Temperature Sensor This temperature sensor located on the detector main board delivered a value outside the allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause Suggested actions 1 The temperature sensor is defect Please contact your Agilent service representative 2 Detector is exposed to illegal ambient Verify that the ambient conditions are within the conditions allowed range Illegal Value From Temperature Sensor This temperature sensor delivered a value outside the allowed range The parameter of this event equals the measured temperature in 1 100 centigrade As a result the temperature control is switched off Probable cause Suggested actions 1 Dirty or defective sensor Please contact your Agilent service representative Links 2 Detector is exposed to illegal ambient Verify that the ambient condit
63. e maintenance and repair procedures for the detector that can be carried out without opening the main cover Table 42 Detector maintenance and repair Procedure Typical Frequency Notes Exchanging the deuterium If noise and or drift exceeds your application limits A VWD test should be performed after lamp or lamp does not ignite replacement Exchanging the flow cell If the application requires a different flow cell type A VWD test should be performed after replacement Repairing the flow cell If leaking or if intensity drops due to contaminated A pressure tightness test should be flow cell windows done after repair Drying the leak sensor If leak has occurred Check for leaks 184 1220 Infinity LC Preventive Maintenance and Repair Exchanging the Deuterium Lamp When If noise or drift exceeds application limits or lamp does not ignite Tools required Screwdriver POZI 1 PT3 Parts required p n Description 1 G1314 60100 Deuterium lamp Preparations Turn the lamp OFF WARNING Injury by touching hot lamp If the detector has been in use the lamp may be hot gt If so wait for lamp to cool down 1 Press the release buttons and remove the lower front 2 Unscrew the heater assembly and remove it cover to have access to the lamp area 1220 Infinity LC 185 6 Preventive Maintenance and Repair Detector 3 Unscrew disconnect and replace the lamp Insert fix and 4 Replace the heater assemb
64. e ripple unstable run leak test for verification Pressure ripple unstable run leak test for verification Solvent dripping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit b ml min H20 with purge open indicates blockage Leaks at lower pump head side unstable retention times pressure ripple unstable run leak test for verification 141 6 Preventive Maintenance and Repair Table 40 Simple repair procedures overview Procedure Typical frequency Notes Removing the pump head Before exchanging the seals assembly or plungers Exchanging the Plungers If scratched Seal life time shorter than normally expected check plungers while changing the seals Instrument is partially energized when switched off The power supply still uses some power even if the switch on the front panel is turned off gt To disconnect the Agilent 1220 Infinity LC pump from line unplug the power cord Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet suppli
65. eat position Lowers the needle into the sample Metering device draws the defined injection volume Lifts the needle out of the vial Comments Command also switches the valve to bypass if it is not already in that position Command also lifts the needle to the upper position Command also positions the vial at the seat and lifts the needle to the upper position Command also positions the vial at the seat lifts the needle and lowers the needle into vial Command can be done more than once maximum draw volume of 100pl cannot be exceeded Use Plunger Home to reset the metering device Command also switches the valve to bypass if it is not already in that position TI 4 Test Functions and Calibration Table 30 Injector Step Commands Step Action Comments Vial to Tray Returns the selected vial to the tray Command also lifts the needle position to the upper position Needle into Seat Lowers the needle arm into the seat Command also returns the vial to the tray position Valve Mainpass Switches the injection valve to the mainpass position Reset Resets the injector Alignment Teaching ALS Alignment Teaching Description The Alignment Teaching tool is required to compensate for small deviations in positioning of the autosampler gripper that may occur after the module has been disassembled for repair It requires that a 100 vial tray be inserted into the autosampler The alignment procedure uses two
66. ed by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components 2 In order to prevent damage always use an ESD protection when handling electronic boards and components 142 1220 Infinity LC Preventive Maintenance and Repair 6 Checking and Cleaning the Solvent Filter WARNING Small particles can permanently block the capillaries and valves of the pump Damage to the Agilent 1220 Infinity LC pump gt Always filter solvents gt Never use the pump without solvent inlet filter If the filter is in good condition the solvent will freely drip out of the solvent tube hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube Cleaning the Solvent Filter When If solvent filter is blocked Parts required Description 1 Concentrated nitric acid 35 1 Bidistilled water 1 Beaker Preparations Remove solvent inlet tube from the Inlet Valve 1 Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 65 for one hour 2 Thoroughly flush the filter with bidistilled water remove all nitric acid some capillary columns can be damaged by nitric acid 3 Replace the filter 1220 Infinity LC 143 6 Preventive Maintenance and Repa
67. edback to be adapted to specific user requirements The wear of autosampler components is dependent on the analytical conditions Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument The autosampler provides two EMF counters Each counter increments with autosampler use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The autosampler provides the following EMF counters Injection valve counter This counter display the total number of switches of the injection valve since the last reset of the counter Needle movements counter This counter displays the total number of movements of the needle into the seat since the last reset of the counter EMF counters for the variable wavelength detector 1220 Infinity LC The user settable EMF limits for the EMF counter enables the early maintenance feedback to be adapted to specific user requirements The useful lamp burn time is dependent on the requirements for the analysis high or low sensitivity analysis wavelength and so on Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument The detector module provides a EMF counter for the lamp The counter increments with lamp use and can be assigned a maximum li
68. embly Suggested actions Re connect the filter motor on the main board Replace the optical unit or main board 127 5 Error Information Grating Filter Motor Test Failed The motor test has failed test Test 0 Failed Filter motor Test 1 Failed Grating motor During the motor tests the detector moves the motor to the end position while monitoring the end position sensor If the end position is not found the error message is generated Probable cause Suggested actions 1 Defective motor assembly Please contact your Agilent service representative Grating Missing The grating motor is not detected Probable cause Suggested actions 1 Grating motor is not connected Re connect the filter motor on the main board 2 Cable connector defective Replace the optical unit or main board 128 1220 Infinity LC Heater Current Missing Error Information 5 The lamp heater current in the detector is missing During lamp ignition the processor monitors the heater current If the current does not rise above the lower limit within one second the error message is generated Probable cause 1 Lamp disconnected 2 Ignition started without the top foam in place 3 Defective VWM board 4 Defective or non Agilent lamp 5 Defective power supply Heater Failed Suggested actions Ensure the lamp is connected Replace the top foam and turn on the lamp Please contact your Agilent service representative
69. ements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product E Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1220 Infinity LC Appendix 9 nformity for HOX2 Fi Declaration of Conformity for HOX2 Filter Declaration of Conformity We herevrith infonm you that the Holmium Oxide Glass Filter Type Hoya HY 1 Part No 79880 22711 meets the following specification of absorbance maxima positions ac orv O De Banian Number Wavdength ccura Bandwidth h Inm mo 79853C 1050 360 8ron i 2 rm 6rm 418 5rm o mmo ee C G1314A B C 1100 1200 360 9rm t l1mm 6rm 418 5rm OL bea O The variation in Measured Wavelength depends on the different Optical Bandvridth Agilent Technologies guarantees the trace ability of the specified absorb ance maxima to a National Institute of Standards amp Technology NIS T Hohmium Oxide Solution Standard with a lot to lot tolerance of 0 3 ram The wavelength calibration filter built into the Agilent Technologies UV VIS detectors is made of this material amd meets these specifications It is therefore s
70. ensity average intensity and lowest intensity across the full wavelength range The test is used to determine the performance of the lamp and optics see also cell test To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The shape of the intensity spectrum is primarily dependent on the lamp and grating Therefore intensity spectra will differ slightly between instruments On completion of the test the intensity spectrum and intensity values are displayed VWD Cell Test Description on page 84 The test should not be performed using the micro flow cell since the reduction in light intensity will cause the test to fail 1220 Infinity LC 87 4 Test Functions and Calibration Intensity Test Report VWD Intensity Spectrum intensity counts 3 2 a JA 2000003 jd d 1750004 i 1500004 125000 Jf 100000 J 75000 j 500004 25000 4 VWD Intensity Test Results Specification Measured Accumulated lamp on time Highest intensity Average intensity Lowest intensity Evaluating the VWD Intensity Test Table 36 Limits Intensity Limits counts Highest gt 320000 Average gt 160000 Lowest gt 6400 gt 320000 ots gt 160000 cts 6400 cts 94 35 h 7123680 cts P assed 951488 cts Passed 36384 cts Passed 1220 Infinity LC Test Functions and Calibration 4 Table 37 Probabl
71. ent Compressibility Solvent pure Compressibility 10 bar Isobutanol 100 Isopropanol 100 Methanol 120 1 Propanol 100 Toluene 87 Water 46 Variable Stroke Volume 1220 Infinity LC Due to the compression of the pump chamber volume each plunger stroke of the pump generates a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation is dependent mainly on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate pressure pulsations of smaller amplitude than larger stroke volumes at the same flow rate In addition the frequency of the pressure pulsations are higher This decreases the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes result in less flow ripple and improve composition ripple The pump uses a processor controlled spindle system to drive its plungers The normal stroke volume is optimized for the selected flow rate Low flow rates use a small stroke volume while higher flow rates use a larger stroke volume When the stroke volume for the pump is set to AUTO mode the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended 45 3 46 Agilent 1220 Infinity LC Description Using the Pump Hints for successful use of the Agilent 1220 Infinity LC pump When using salt solutions and organic solvents in
72. etected within the scan range the error message is generated Probable cause 1 Lamp is OFF 2 Incorrect flow cell installation 3 Flow cell contamination or air bubbles 4 Intensity too low 5 Current step value too far from maximum Suggested actions Switch on the lamp Ensure the flow cell are installed correctly Clean replace flow cell windows or remove air bubbles Replace lamp Enter a different calibration step value different scan range Repeat the calibration 1220 Infinity LC Probable cause 6 Misaligned defective grating assembly 7 ProbableCause Filter Check Failed Error Information 5 Suggested actions Run the grating motor test to determine if the grating assembly is defective If defective replace the optical unit Exchange the VWM board The automatic filter check after lamp ignition has failed When the lamp is switched on the detector moves the cutoff filter into the light path If the filter is functioning correctly a decrease in lamp intensity is seen If the expected intensity decrease is not detected the error message is generated Probable cause 1 Filter motor defective 2 Defective or missing filter Filter Missing The filter motor is not detected Probable cause 1 Filter motor is not connected 2 Cable connector defective 1220 Infinity LC Suggested actions Run the filter motor test to determine if the motor is defective Exchange the filter ass
73. g a minimum of 15 000 injections with the Agilent 1220 Infinity LC autosampler Table 23 Crimp Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5181 3375 5183 4491 Clear glass 2 5182 0543 5183 4492 5183 4494 write on spot Amber glass 2 5182 3376 5183 4493 5183 4495 write on spot Polypropylene 1 5182 0567 5183 4496 wide opening Polypropylene 0 3 9301 0978 wide opening Table 24 Snap Top Vials continued Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0544 5183 4504 5183 4507 Clear glass 2 5182 0546 5183 4505 5183 4508 write on spot Amber glass 2 5182 0545 5183 4506 5183 4509 write on spot 59 3 60 Agilent 1220 Infinity LC Description Table 25 Screw Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0714 5183 2067 5183 2070 Clear glass 2 5182 0715 5183 2068 5183 2071 write on spot Amber glass 2 5182 0716 5183 2069 5183 2072 write on spot Table 26 Crimp Caps Description Septa 100 Pack Silver aluminum Clear PTFE red rubber 5181 1210 Silver aluminum Clear PTFE red rubber 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber 5181 1215 Green aluminum Clear PTFE red rubber 5181 1216 Red aluminum Clear PTFE red rubber 5181 1217 Table 27 Snap Caps Description Septa 100 Pack Clear polypropylene Clear PTFE red rubber 5182 05
74. ge the needle drive motor 3 Sticking spindle assembly Exchange the spindle assembly or sampling unit assembly 4 Defective ASM board Exchange ASM board Safety Flap Missing The safety flap was not detected Before the needle moves down into the needle seat to inject sample the safety flap locks into position Next and the gripper checks the safety flap by trying to move the safety flap away from the needle If the gripper is able to move beyond the safety flap position safety flap not in position the error message is generated Probable cause Suggested actions 1 Safety flap missing or broken Exchange the safety flap 1220 Infinity LC 123 5 Error Information Valve to Bypass Failed The injection valve failed to switch to the bypass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Defective injection valve Exchange the injection valve 2 Defective ASM board Exchange the ASM board Valve to Mainpass Failed The injection valve failed to switch to the mainpass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fa
75. ging the Gripper Arm When Defective gripper arm Tools required Straightened paper clip Parts required p n Description 1 G1313 60010 Gripper assembly Preparations Select Start in the Tools function in the LMD Software ChangeGripper Turn off the power to the Instrument Remove the upper front cover 1 Identify the slit below the gripper motor and the gripper 2 Rotate the arm approximately 2 5cm 1 inch to the left arm release button and insert the straightened paper clip into the slit 1 2 e CO qw NUS DG 1220 Infinity LC 181 6 Preventive Maintenance and Repair Autosampler 3 Rotate the gripper arm slowly from left to right and apply a 4 Hold the paper clip in place press the gripper release gentle pressure to the paper clip The clip will engage on button and rotate the gripper arm to the right an internal catch and the rotation of the arm will be blocked 182 1220 Infinity LC Preventive Maintenance and Repair 6 Autosampler 5 The gripper arm will come off 6 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left Next Steps 7 On completion of this procedure Install the front cover 8 Turn the power to the Instrument ON 1220 Infinity LC 183 6 Preventive Maintenance and Repair Detector Introduction This section describes simpl
76. head 157 recommended pH range 14 reference conditions 16 repair procedures injection seal 161 repairs metering plunger 177 metering seal 177 needle assembly 166 needle seat assembly 170 overview of simple repairs 184 rotor seal 173 restart without cover 111 rotor seal exchange 173 running the leak test 73 79 S safety class 222 safety features system 13 safety general information 222 symbols 224 sample loops 48 sample trays 58 numbering of vial positions 58 sample volume 49 sampling sequence 51 sampling unit 55 sapphire piston 40 40 screwdriver pozidriv 1 158 236 seal wear counters 138 seals 177 selection valve failed 110 servo restart failed 112 setable flowrange 13 shut down 103 simple repairs autosampler 164 site requirements power cords 10 site requirements bench space 10 environment 11 power considerations 9 solvent delivery system 39 solvent inlet filters 46 Solvent Selection Valve 8 solvent filters checking 143 cleaning 143 prevent blockage 46 solvent information 225 specifications 14 performance 16 physical 12 SSV 8 standards 12 stator 56 step commands 77 stepper motor 55 storing the settings permanently 28 stroke length 112 stroke volume 40 45 synchronization lost 104 system configurations 8 T TCP IP parameter configuration 24 telnet configuration 30 temperature limit exceeded 113 temperature out of range 113 temperature sensor 101 temperature 14 tests wavelength cal
77. humidity as described in the following sections ASTM drift tests require a temperature change below 2 C hour 3 6 F hour measured over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 1220 Infinity LC 11 1 Introduction Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 30 kg 66 Ibs Dimensions 640x370x420 mm height x width x depth Line voltage Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL EN Housing 25 2x14 6x16 5 inches 100 240 VAC 10 Wide ranging capability 50 or 60 Hz 5 240 VA
78. ibration 90 theta axis 57 timeout 104 tools screwdriver pozidriv 1 158 transport assembly 57 transport mechanism 51 troubleshooting error messages 98 U unknown error 2055 116 URL 232 using the cuvette holder 192 UV radiation 228 V vacuum degasser 46 valve frit 148 valve proportioning 39 variable reluctance motor 40 variable stroke volume 45 verifying the gripper position 77 vial contents temperature 14 vial numbering 58 vialracks 51 vials 51 59 VWD EMF counters 139 1220 Infinity LC Index WwW wait timeout 116 waste electrical and electronic equipment 230 wavelength accuracy 16 calibration 90 range 190 600nm 16 WEEE directive 230 weight 12 wrench 1 4 inch 73 79 148 151 151 152 152 155 155 157 157 wrench 14 mm 144 148 X X axis 57 Z Z axis 57 zero solvent counter 116 1220 Infinity LC 237 www agilent com In This Book This manual contains information on how to use maintain repair and upgrade the Agilent 1220 Compact LC System The manual contains the following chapters Introduction Installation Agilent 1220 Infinity LC Description Test Functions and Calibratiojn Error Information Preventive Maintenance and Repair Parts for Maintenance and Repair Upgrading the Agilent 1220 Infinity LC Appendix O Agilent Technologies 2010 Printed in Germany 07 10 G4280 90010 RES Agilent Technologies
79. ical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals plungers and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Exchange the pump drive assembly Index Missing The encoder index position in the module was not found during initialization During initialization the first plunger is moved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable cause Suggested actions 1 Disconnected or defective encoder cable Ensure the encoder cable are not damaged or dirty Make sure the cables are connected securely to the main board 2 Defective pump drive assembly Exchange the pump drive assembly 106 1220 Infinity LC Error Information 5 Initialization Failed The module failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable cause
80. ill present Seal 4 Insert new seals into the pump head Pump head Support ring Plunger housing 1220 Infinity LC 153 6 Preventive Maintenance and Repair CAUTION Seal Wear in Procedure This procedure is required for standard seals only 5063 6589 It will damage the normal phase application seals 0905 1420 gt Never perform the seal wear in procedure with normal phase application seals 154 1 Place a bottle with 100 ml of Isopropanol in the solvent cabinet and attach a bottle head assembly including tubing to the bottle 2 Connect the inlet tube from the bottle head directly to the Inlet Valve 3 Connect one end of the restriction capillary 5022 2159 to the purge valve Insert the other end into a waste container 4 Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 mL min 5 Close the purge valve and set the flow to a rate adequate to achieve a pressure of 350 bar 6 Pump for 15 minutes at this pressure to wear in the seals 7 Turn off the pump and slowly open the purge valve to release the pressure from the system 8 Disconnect the restriction capillary and reinstall the bottle containing the solvent for your application 9 Rinse your system with the solvent used for your application 1220 Infinity LC Preventive Maintenance and Repair 6 Exchanging the Plungers When When scratched Tools required Wrench 1 4 in
81. ils to reach the mainpass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Defective injection valve Exchange the injection valve 2 Defective ASM board Exchange the ASM board 124 1220 Infinity LC Error Information 5 Vial in Gripper The gripper arm attempted to move with a vial still in the gripper During specific stages of the sampling sequence no vial should be held by the gripper The autosampler checks if a sample vial is stuck in the gripper by closing and opening the gripper fingers while monitoring the motor encoder If the gripper fingers are unable to close the error message is generated Probable cause Suggested actions 1 Vial still in gripper Remove the vial using the Release Vial function in the user interface Reinitialize the autosampler 1220 Infinity LC 125 5 Error Information Detector Error Messages 126 These errors are specific to the variable wavelength detector ADC Hardware Error A D Converter hardware is defective Probable cause 1 A D Converter hardware is defective Calibration Failed Suggested actions Replace the optical unit The intensity maximum was not found during wavelength calibration Calibration 0 Failed Zero order calibration failed Calibration 1 Failed 656 nm calibration failed During zero order and 656 nm calibration the detector searches for the intensity maximum If the maximum is not d
82. in place in the Agilent 1220 Infinity LC These repairs are described in following sections Overwiew of procedures Procedure Typical Frequency Time Required Exchanging the needle When needle shows indication of damage 15 minutes assembly or blockage Exchanging the seat assembly When the seat shows indication of damage 10 minutes or blockage Exchanging the rotor seal After approximately 30000 to 40000 30 minutes injections or when the valve performance shows indication of leakage or wear Exchanging the metering seal When autosampler reproducibility 30 minutes indicates seal wear Exchanging the gripper arm When the gripper arm is defective 10 minutes The power supplies still use some power even if the power switch on the front panel is turned off Repair work at the autosampler can lead to personal injuries e g shock hazard when the autosampler cover is opened and the instrument is connected to power Make sure that it is always possible to access the power plug Remove the power cable from the instrument before opening the cover Do not connect the power cable to the Instrument while the covers are removed 164 1220 Infinity LC Preventive Maintenance and Repair 6 Exchanging Internal Parts Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the autosampler unit from the Agilent 1220 Infinity LC these repairs have to be done by trained service personal
83. instrument The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrument from a power outlet that has no ground connection Never use a power cord other than the Agilent Technologies power cord designed for your region Use of cables not supplied by Agilent Using cables that have not been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 10 Bench Space The dimensions and weight of the Agilent 1220 Infinity LC allow it to be placed on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches at the rear for air circulation and electric connections Make sure that the bench intended to carry the Agilent 1220 Infinity LC is designed to bear the weight of the instrument The Agilent 1220 Infinity LC should be operated upright 1220 Infinity LC Introduction 1 Environment CAUTION Your Agilent 1220 Infinity LC will work within specifications at ambient temperatures and relative
84. ion If the anode voltage falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective or non Agilent lamp Exchange the lamp 2 Defective power supply Please contact your Agilent service representative 3 Defective VWM board Please contact your Agilent service representative Wavelength Check Failed The automatic wavelength check after lamp ignition has failed When the lamp is switched on the detector waits 1 minute to warm up the lamp Then a check of the deuterium emission line 656 nm via the reference diode is performed If the emission line is more than 3 nm away from 656 nm the error message is generated Probable cause Suggested actions 1 Calibration incorrect Recalibrate the detector 1220 Infinity LC 133 5 Error Information Detector Error Messages 134 1220 Infinity LC 1220 Infinity LC 6 Preventive Maintenance and Repair PM Scope of Work and Checklist 137 Early Maintenance Feedback 138 EMF counters for the pump 138 EMF counters for the autosampler 139 EMF counters for the variable wavelength detector 139 Solvent Delivery System 141 Introduction 141 Checking and Cleaning the Solvent Filter 143 Exchanging the Passive Inlet Valve 144 Exchanging the Outlet Ball Valve 146 Exchanging the Purge Valve Frit or the Purge Valve 148 Removing the Pump Head Assembly 151 Exchanging the Pump Seals and Seal Wear in Procedure 152 Exchanging the Plungers 155 Reinstalling the P
85. ions are within the conditions allowed range 1220 Infinity LC 131 5 Error Information Lamp Current Missing The lamp anode current is missing The processor continually monitors the anode current drawn by the lamp during operation If the anode current falls below the lower current limit the error message is generated Probable cause 1 Lamp disconnected 2 Top foam removed while lamp is on 3 Defective or non Agilent lamp 4 Defective VWM board 5 Defective power supply Lamp Ignition Failed Suggested actions Ensure the lamp connector is seated firmly Replace the top foam and turn ON the lamp Exchange the lamp Please contact your Agilent service representative Please contact your Agilent service representative The lamp failed to ignite The processor monitors the lamp current during the ignition cycle If the lamp current does not rise above the lower limit within 2 5 seconds the error message is generated Probable cause 1 Lamp disconnected 2 Defective or non Agilent lamp 3 Defective power supply 4 Defective VWM board 132 Suggested actions Ensure the lamp is connected Exchange the lamp Please contact your Agilent service representative Please contact your Agilent service representative 1220 Infinity LC Error Information 5 Lamp Voltage Missing The lamp anode voltage is missing The processor continually monitors the anode voltage across the lamp during operat
86. ir Exchanging the Passive Inlet Valve When If leaking internally backflow Tools required Wrench 14 mm Pair of Tweezers Parts required Description G1312 60066 Passive Inlet Valve Preparations Place the solvent bottles under the pump 1 Remove the upper front cover 2 Disconnect the solvent inlet tube from the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow 3 Unscrew the adapter from the inlet valve optional 4 Using a 14 mm wrench loosen the inlet valve and remove the valve from pump head Figure 25 Passive Inlet Valve 144 1220 Infinity LC Preventive Maintenance and Repair 6 Passive Inlet Valve part number G4280 60036 O Ring part number 0905 1684 5 Insert the new valve into the pump head 6 Using the 14 mm wrench turn the nut until it is hand tight 7 Reconnect the adapter at the inlet valve optional 8 Reconnect the solvent inlet tube to the adapter Reinstall the front cover After an exchange of the valve it may take several ml of pumping with the solvent used in the current application before the flow stabilizes at a ripple as low as it used to be when the system was still working properly 1220 Infinity LC 145 6 Preventive Maintenance and Repair Exchanging the Outlet Ball Valve When Tools required Parts required Preparations 146 If leaking internally Wrench 1 4 inch Description G1312 60067 Outlet ball valv
87. is no mechanical blockage of the pump head assembly or pump drive assembly 3 Defective pump drive assembly Exchange the pump drive assembly 4 Defective main board Exchange the main board Stroke Length The distance between the lower plunger position and the upper mechanical stop is out of limits pump During initialization the module monitors the drive current If the plunger reaches the upper mechanical stop position before expected the motor current increases as the module attempts to drive the plunger beyond the mechanical stop This current increase causes the error message to be generated Probable cause Suggested actions 1 Defective pump drive assembly Exchange the pump drive assembly 1220 Infinity LC Error Information 5 Temperature Limit Exceeded The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the main board If excessive current is being drawn for long periods the temperature of the circuits increases If the temperature exceeds the upper limit the error message is generated Probable cause Suggested actions 1 High friction partial mechanical blockage in Ensure the capillaries and frits between the the pump drive assembly pump head and damper inlet are free from blockage 2 Partial blockage of the flowpath in front of Ensure the outlet valve is not blocked the damper 3 Defective pump drive assembly
88. ity of the solvents in use affects retention time stability when the back pressure in the system changes for example ageing of the column To minimize this effect the pump provides a compressibility compensation feature that optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without compressibility compensation the following happens during a stroke of the first plunger the pressure in the plunger chamber increases and the volume in the chamber is compressed depending on backpressure and solvent type The volume displaced into the system is reduced by the compressed volume When a compressibility value is set the processor calculates a compensation volume that is depending on the backpressure in the system and the selected compressibility This compensation volume is added to the normal stroke volume and compensates for the previously described loss of volume during the delivery stroke of the first plunger Optimizing the compressibility compensation setting The default compressibility compensation setting is 46 x 10 bar This setting represents an average value Under normal conditions the default setting reduces the pressure pulsation to values below 1 of system pressure that 43 3 Agilent 1220 Infinity LC Description are sufficient for most applications and for all gradient analyses For applications using sensit
89. ive detectors the compressibility settings can be optimized by using the values for the various solvents If the solvent in use is not listed in the compressibility tables when using isocratic mixtures of solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings When using mixtures of solvents it is not possible to calculate the compressibility of the mixture by interpolating the compressibility values of the pure solvents used in that mixture or by applying any other calculation In these cases the following empirical procedure has to be applied to optimize your compressibility setting 1 Start the pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Use degassed solvent only Check the tightness of the system with the pressure test 3 Your pump must be connected to control software with which the pressure and ripple can be monitored 4 The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition Table 19 Solvent Compressibility Solvent pure Compressibility 10 bar Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 44 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Table 19 Solv
90. jection seal Always rinse the valve with water after using aqueous buffers or salt solutions 1 Switch the valve to the INJECT position 2 Use the pump to flush the sample loop and seal grooves 3 Use the needle port cleaner supplied with the valve and syringe to flush the needle port and vent capillary 160 1220 Infinity LC Preventive Maintenance and Repair 6 Exchanging the Injection Valve Seal When Poor injection volume reproducibility Leaking injection valve Tools required Hex key 9 64 inch supplied in the tool kit Parts required Description Rotor seal Vespel 1 Loosen the three stator screws Remove the stator head 2 Remove the stator ring 1220 Infinity LC 161 Preventive Maintenance and Repair 3 Remove the rotor seal 5 Install the stator ring Ensure the pin in the stator ring is 4 Install the new rotor seal 6 aligned with the hole in the valve body Install the stator head onto the valve 162 1220 Infinity LC Preventive Maintenance and Repair 6 7 Secure stator head in place with the stator screws Tighten each screw alternately 74 turn until the stator head is secure 1220 Infinity LC 163 6 Preventive Maintenance and Repair Autosampler Introduction The autosampler is designed for easy repair The most frequent repairs can be done from the front of the instrument with the instrument
91. jection valve switches to the bypass position 51 3 52 Agilent 1220 Infinity LC Description 2 The plunger of the metering device moves to the initialization position w ce N c cw A The gripper arm selects the vial At the same time the needle lifts out of the Seat The gripper arm places the vial below the needle The needle lowers into the vial The metering device draws the defined sample volume The needle lifts out of the vial If the automated needle wash is selected the gripper arm replaces the sample vial positions the wash vial below the needle lowers the needle into the vial then lifts the needle out of the wash vial The gripper arm checks if the safety flap is in position 10 The gripper arm replaces the vial Simultaneously the needle lowers into the seat 11 The injection valve switches to the mainpass position Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position In this position the mobile phase flows through the autosamplers metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over 1220 Infinity LC 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Injection System Figure 18 Mainpass Position When the sample sequence begins the valve unit switches to the bypass position Solvent from the pump enters the
92. l using the Release Vial function in the user interface Reinitialize the autosampler 1220 Infinity LC Invalid Vial Position Error Information 5 The vial position defined in the method or sequence does not exist The reflection sensors on the transport assembly flex board are used to check automatically which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated Probable cause 1 Incorrect tray or trays installed 2 Incorrect vial positions defined in the method or sequence 3 Tray recognition defective dirty sample tray or defective transport assembly flex board Metering Home Failed 1220 Infinity LC Suggested actions Install the correct trays or edit the method or sequence accordingly Exchange the transport assembly Ensure the coding surfaces of the sample tray are clean located at the rear of the sample tray The metering plunger has failed to move back to the home position The home position sensor on the sampling unit flex board monitors the home position of the plunger If the plunger fails to move to the home position or if the sensor fails to recognize the plunger position the error message is generated Probable cause 1 Dirty or defective sensor 2 Broken plunger 3 Defective metering drive motor 4 Defective ASM board Suggested actions Exchange the sampling unit flex board
93. let ball valve Exchange the outlet ball valve 3 High friction partial mechanical blockage in Remove the pump head assembly Ensure there the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 4 Defective pump drive assembly Exchange the pump drive assembly 5 Defective main board Exchange the main board 108 1220 Infinity LC Error Information 5 Pressure Above Upper Limit The system pressure has exceeded the upper pressure limit Probable cause Suggested actions 1 Upper pressure limit set too low Ensure the upper pressure limit is set to a value suitable for the analysis 2 Blockage in the flowpath after the damper Check for blockage in the flowpath The following components are particularly subject to blockage purge valve frit needle autosampler seat capillary autosampler sample loop autosampler column frits and capillaries with low internal diameters e g 0 12 mm id 3 Defective damper Exchange the damper 4 Defective main board Exchange the main board Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit Probable cause Suggested actions 1 Lower pressure limit set too high Ensure the lower pressure limit is set to a value suitable for the analysis 2 Leak Inspect the pump head capillaries and fittings for signs of a leak Purge the module Run a pressure test to determine whether the seals or other
94. ll the head assembly away from the sampler Notice that the closed side of the metering head faces upwards 1220 Infinity LC 177 6 Preventive Maintenance and Repair 3 Remove the two fixing bolts from the base of the metering head assembly 5 Use a small screwdriver to carefully remove the seal Clean the chamber with lint free cloth Ensure all particular matter is removed 4 Disassemble the metering head assembly 6 Install the new seal Press the seal firmly into position 178 1220 Infinity LC Preventive Maintenance and Repair 6 7 Place the piston guide on top of the seal 8 Reassemble the metering head assembly Carefully insert the plunger into the base The closed side of the metering head must be on the same side as the lower one of the two capillary drillings 2 9 Install the fixing bolts Tighten the bolts securely 10 Install the metering head assembly in the autosampler Ensure the large hole in the metering head is facing downwards VU t 2 S La fm 1220 Infinity LC 179 6 Preventive Maintenance and Repair Autosampler 11 Reinstall the capillaries Next Steps 12 On completion of this procedure Install the front cover 13 Select End in the Tools function in the LMD Software Change piston 180 1220 Infinity LC Preventive Maintenance and Repair 6 Autosampler Exchan
95. ls Double beam photometer Deuterium lamp 190 600 nm 0 35 x 10 AU at 230 nm 3 x 10 AU hr at 254 nm gt 2 AU 5 upper limit tinm 6 5 nm typical Standard 14 pl volume 10 mm cell path length and 40 bar 588 psi maximum pressure High pressure 14 pl volume 10 mm cell path length and 400 bar 5880 psi maximum pressure Semi micro 5 ul volume 6 mm cell path length and 40 bar 588 psi maximum pressure Micro 2 ul volume 3 mm cell path length and 40 bar 588 psi maximum pressure 2 sec time constant under specified conditions See NOTE below the table See NOTE below the table Self calibration with deuterium lines verification with holmium oxide filter Can be repaired on component level ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions cell path length 10 mm response time 2 s flow 1 ml min LC grade methanol Linearity measured with caffeine at 272 nm nm 16 1220 Infinity LC P 1220 Infinity LC c00 ee 2 099 Installation o e o e e e Unpacking Your System 18 e LAN Configuration 22 To dofirst 22 TCP IP parameter configuration 24 Configuration Switches 24 Initialization mode selection 25 Link configuration selection 28 Storing the settings permanently with Bootp 28 Manual Configuration 29 This chapter provides an overview on shipment content and installation NOTE 7 To install the Agilen
96. lunger seals pressurize the system according to the wear in procedure Preventing blockage of solvent filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the pump This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong the lifetime of the solvent filter and will maintain the performance of the pump 1220 Infinity LC Agilent 1220 Infinity LC Description 3 e Use a sterile if possible amber solvent bottle to slow down algae growth Filter solvents through filters or membranes that remove algae Exchange solvents every two days or refilter Ifthe application permits add 0 0001 0 001M sodium azide to the solvent Place a layer of argon on top of your solvent Avoid exposure of the solvent bottle to direct sunlight Never use the system without a solvent filter installed 1220 Infinity LC 47 3 Agilent 1220 Infinity LC Description Injection System This chapter provides an overview of the operational principles of the Injection Systems Manual Injector and Autosampler Manual Injector The Agilent 1220 Infinity LC manual injector uses a Rheodyne 6 port sample injection valve part number 5067 4202 Sample is loaded into the external 20 ul sample loop through the injection port at the front of the valve The valve has a PEEK injection seal A make befo
97. ly and column filter causing increased system pressure Algae appear as white or yellowish white deposits on filters Typically black particles from the normal wear of the piston seals do not cause the PTFE frits to block over short term use 195 196 Preventive Maintenance and Repair Short lifetime of solvent filters bottle head assembly A blocked solvent filter in the bottle especially when only partly blocked is more difficult to identify and may show up as problems with gradient performance intermittent pressure fluctuations etc Algae growth may also be the possible source of failures of the ball valves and other components in the flow path Preventing and or reducing the algae problem Always use freshly prepared solvents especially use demineralized water that has been filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle part number 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase 1220 Infinity LC 1220 Infinity LC 7 Parts for Maintenance and Repair Agilent 1220 Infinity LC System 198 Solvent Delivery System 200 Injection System 208 Manual Injector 208 Autosampler 210 Column Oven 215 Detector 216 RE Agilent Technologies 197 7 Parts for Mainten
98. ly reconnect the lamp Replace the front cover Reset the lamp counter as described in the Utilities software documentation Give the lamp more than 10 minutes to warm up 5 6 7 Turn the lamp ON 8 9 Perform Wavelength Calibration to check the correct positioning of the lamp 186 1220 Infinity LC Preventive Maintenance and Repair 6 Exchanging a Flow Cell When If the application requires a different type of flow cell or the flow cell needs maintenance Tools required Two 1 4 inch wrenches for capillary connections Preparations Turn the lamp OFF 1 Press the release buttons and remove the lower front 2 Disconnect the inlet and outlet capillaries cover to gain access to the flow cell area 1220 Infinity LC 187 6 Preventive Maintenance and Repair Detector 3 Unscrew the thumb screws in parallel and remove the flow cell 4 Insert the new flow cell and fix the thumb screws 188 1220 Infinity LC Preventive Maintenance and Repair 6 Next Steps 6 To check for leaks establish a flow and observe the flow cell outside of the cell compartment and all capillary connections 7 Replace the front cover 1220 Infinity LC 189 Preventive Maintenance and Repair Repairing the Flow Cell Parts required oN oorwon 190 G1314 60086 G1314 65063 Cell screw Conical springs Ring 1 PEEK Gasket 1 small hole Window Quartz
99. m above the seat 4 Connect the seat capillary fitting to port 5 of the injection valve 6 Ensure the needle is aligned with the seat If required bend the needle slightly until the needle is aligned correctly 1220 Infinity LC 171 6 Preventive Maintenance and Repair Next Steps 7 On completion of this procedure Install the front cover 8 Select End in the Tools function Change Needle 172 1220 Infinity LC Preventive Maintenance and Repair 6 Exchanging the Rotor Seal When Poor injection volume reproducibility Leaking injection valve Tools required 1 4 inch wrench supplied in the tool kit Hex key 9 16 inch supplied in the tool kit Parts required p n Description 1 0100 1853 Rotor seal Vespel 1 0100 1849 Rotor seal Tefzel 1 0101 1416 Rotor seal PEEK Preparations Remove upper front cover Remove the leak tubing if necessary CAUTION Removing the stator head The stator face is held in place by the stator head When you remove the stator head the stator face can fall out of the valve gt Carefully handle the valve to prevent damage to the stator face 1220 Infinity LC 173 6 Preventive Maintenance and Repair Autosampler 1 Remove all capillary fittings from the injection valve ports 2 Loosen each fixing bolt two turns at a time Remove the bolts from the head VQ 3 Remove the stator head and stator ring
100. m lies in the needle needle loop or metering drive otherwise the problem lise in the needle seat or needle seat capillary If the autosampler is not the source of the problem the problem lies either in the pump typically the filter or frit or after the autosampler heater capillary or column If the pressure drop limit is not achieved the problem lies in the pump otherwise the problem occurs after the autosampler Purge Pump 1220 Infinity LC Purge Pump Description The Purge Pump tool enables you to purge the pump with solvent at a specified flow rate for a specified time For multi channel pumps and pumps with solvent selection valve SSV you select the channels to purge each channel can be purged with different conditions You can select a flow rate between 1 and 5 mL min in steps of 1 mL min You can select a time from 1 2 3 5 7 10 and 15 minutes The G1361A Prep Pump has an automatic purge cycle there are no user configurable options Purging the Pump To purge the pump 1 Prepare each channel with the appropriate purge solvents 2 Select Purge Pump from the tool selection screen 3 In the Purge Configuration dialog box If necessary select the channel s that you want to purge For each selected channel select a Flow and a purge Time Click OK to close the Purge Configuration dialog box 4 When the request to open the purge valve appears open the purge valve on the pump then click OK to close the mes
101. ment or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light also stimulates their growth Small algae growth is seen even in distilled water after some time Instrumental problems associated with algae Algae deposit and grow everywhere within the HPLC system causing deposits on ball valves inlet or outlet resulting in unstable flow or total failure of the pump small pore solvent inlet filters to plug resulting in unstable flow or total failure of the pump small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure column filters to plug resulting in high system pressure flow cell windows of detectors to become dirty resulting in higher noise levels Since the detector is the last module in the flow path this problem is less common Symptoms observed with the Agilent 1220 Infinity LC In contrast to other HPLC systems such as HP 1090 and HP 1050 Series which use helium degassing algae have a better chance to grow in systems such as the Agilent 1220 Infinity LC where helium is not used for degassing most algae need oxygen and light for growth The presence of algae in the Agilent 1220 Infinity LC can cause the following to occur Blockage of PTFE frits part number 01018 22707 purge valve assemb
102. mit which provides visual feedback in the user interface when the limit is exceeded The counter can be reset to zero after the lamp is exchanged The detector provides the following EMF counters 139 6 Preventive Maintenance and Repair arly Maintenance ree Iback Deuterium lamp on time This counter shows the total burn time of the deuterium lamp in hours 140 1220 Infinity LC Preventive Maintenance and Repair 6 Solvent Delivery System Introduction 1220 Infinity LC The Agilent 1220 Infinity LC pump is designed for easy repair The procedures described in this section can be done with the pump in place in the rack The most frequent repairs such as exchanging the plunger seals or purge valve seal can be done from the front of the pump Table 40 Simple repair procedures overview Procedure Typical frequency Notes Checking and cleaning the solvent filter Exchanging the Passive Inlet Valve Exchanging the Outlet Ball Valve Exchanging the Purge Valve Frit or the Purge Valve Exchanging the Purge Valve Frit or the Purge Valve Exchanging the Pump Seals Seal Wear in Procedure If solvent filter is blocked If internally leaking If internally leaking If internally leaking If the frit shows indication of contamination or blockage If pump performance indicates seal wear After exchanging the pump seals Gradient performance problems intermittent pressure fluctuations Pressur
103. nance and Repair Autosampler 5 Insert the new needle 1 Align the needle in the seat 6 Reconnect the sample loop fitting to the needle fitting then tighten the screw firmly 2 7 Use Needle Up to lift the needle to a position approximately 2mm above the seat I i 168 1220 Infinity LC Preventive Maintenance and Repair 6 Next Steps 9 Oncompletion of this procedure Install the front cover 10 Select End in the Tools function Change Needle 1220 Infinity LC 169 6 Preventive Maintenance and Repair Exchanging the Needle Seat Assembly When When the seat is visibly damaged When the seat capillary is blocked Tools required 1 4 inch wrench supplied in accessory kit Flat head screwdriver Parts required p n Description 1 G1313 87101 Needle seat assy 0 17 mm i d 2 3 pl Preparations Select Start in the Tools function in LMD Software Change Needle Remove the upper front cover Use the Needle Up command in the Change Needle function to lift the needle an addition 1 cm 1 Disconnect the seat capillary fitting from the injection 2 Usea small flat head screwdriver to ease out the needle valve port 5 seat 170 1220 Infinity LC Preventive Maintenance and Repair 6 3 Insert the new needle seat assembly Press the seat firmly into position 5 Use Down to position the needle approximately 2 m
104. njector Parts for Maintenance and Repair 7 Description Stainless Steel PEEK Sample loop 5 uL 0101 1248 0101 1241 Sample loop 10 pL 0100 1923 0101 1240 Sample loop 20 uL 0100 1922 0101 1239 Sample loop 50 pL 0100 1924 0101 1238 Sample loop 100 pL 0100 1921 0101 1242 Sample loop 200 pL 0101 1247 0101 1227 Sample loop 500 pL 0101 1246 0101 1236 Sample loop 1 mL 0101 1245 0101 1235 Sample loop 2 mL 0101 1244 0101 1234 209 7 210 Parts for Maintenance and Repair Autosampler Autosampler Main Assemblies Table 52 Autosampler main assembly part numbers Description Part Number Autosampler complete 64280 60230 Transport assembly 61329 60009 Sampling unit assembly excluding injection 64280 60027 valve and analytical head Analytical head assembly 01078 60003 Injection valve assembly 0101 1422 Vial tray 61313 44510 Gripper assembly 61313 60010 Waste capillary 64280 87304 Cable sampling unit 64280 81615 Cable sample transport 64280 81616 Needle port 5067 1581 Analytical Head Assembly Table 53 Analytical head 100 uL part numbers Item Description Part Number Complete analytical head 01078 60003 assembly 1 Plunger assembly 5063 6586 2 Screw M4 40 mm long 0515 0850 3 Adapter 01078 23202 4 Support seal assembly 5001 3739 5 Metering seal pack of 2 5063 6589 1220 Infinity LC 1220 Infinity LC Parts for Maintenance and Repair 7 Table 53 Analytical head 100 pL part num
105. ns 1 Pump head not installed correctly screws Install the pump head correctly Ensure nothing not secured or pump head not seated e g capillary is trapped between the pump correctly head and body 2 Broken plunger Exchange the plunger Restart Without Cover The module was restarted with the top cover and foam open The sensor on the main board detects when the top foam is in place If the module is restarted with the foam removed the module switches off within 30 s and the error message is generated Probable cause Suggested actions 1 The module started with the top cover and Reinstall the top cover and foam foam removed 1220 Infinity LC 111 5 112 Error Information Servo Restart Failed The pump motor in the module was unable to move into the correct position for restarting When the module is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Ensure the pump assembly cables are not damaged or dirty Make sure the cables are connected securely to the main board 2 Mechanical blockage of the module Remove the pump head assembly Ensure there
106. nt through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service board representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative 1220 Infinity LC 101 5 Error Information Leak Sensor Short The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective flow sensor 2 Leak sensor incorrectly routed being pinched by a metal component Open Cover The top foam has been removed Probable cause 1 Foam not activating the sensor 2 Dirty or defective sensor 102 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Suggested actions Please contact your Agilent service representative Please contact your Agilen
107. on 2 Using Default When Using Default is selected the factory default parameters are taken These parameters enable a TCP IP connection to the LAN interface without further configuration Default Parameter Figure 5 Using Default Principle Using the default address in your local area network may result in network problems Take care to change it to a valid address immediately Table 14 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Since the default IP address is a so called local address it is not routed by any network device Thus the PC and the Agilent 1220 Infinity LC must reside in the same subnet You may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the Agilent 1220 Infinity LC You may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the Agilent 1220 Infinity LC is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network you may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the Agilent 1220 Infinity LC are not cleared automatically If you do not change them they are still available when switching back to the Using Stored mode 27
108. on defined in the Install the wash vial in the correct position or method edit the method accordingly 120 1220 Infinity LC Motor Temperature Error Information 5 One of the motors of the transport assembly has drawn excessive current causing the motor to become too hot The processor has switched OFF the motor to prevent damage to the motor See figure Figure 22 on page 57 for motor identification Motor 0 temperature X axis motor Motor 1 temperature Z axis motor Motor 2 temperature Theta gripper rotation motor Motor 3 temperature Gripper motor motor for gripper fingers The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable cause 1 1220 Infinity LC Mechanical obstruction High friction in the transport assembly Motor belt tension too high Defective motor Defective transport assembly flex board Suggested actions Ensure unobstructed movement of the transport assembly Exchange the transport assembly Switch OFF the autosampler at the power switch Wait at least 10 minutes before switching on again Exchange the transport assembly Exchange the transport assembly 121 5 Error Information Needle Down
109. opening a Telnet session 1 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and click OK 2 Type the following at the system DOS prompt C N telnet IP address gt or c gt telnet host name gt E C AWINDOWS system32 cmd exe C gt telnet 134 40 27 95 Figure 7 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle or the default IP address When the connection was established successfully the Agilent 1220 Infinity LC responds with the following ct Telnet 1xx xx xx xx Agilent Technologies G4296A DEGHHHHHHH gt Figure 8 A connection to the module is made 3 Type and press enter to see the available commands 30 1220 Infinity LC Installation 2 ex Telnet 15x xx xx xx Agilent Technologies G4296A DEGHHHHHHH gt description lay help info display current LAN settings ip lt x x x x gt set IP Addr sm amp x x x x t Subnet Mas set Default Gateway exit shell Figure 9 Telnet Commands Table 16 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X X gt sets new ip address sm lt X X X X gt sets new subnet mask gw lt X X X X gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter use the syntax parameter value for example ip 134 40 27 230 Then pre
110. or Year and Week MAC 0030D31210A0 of assembly MAC address Made in Germany Country of Origin 1220 Infinity LC Installation 2 LAN Configuration 2 Connect the instrument s LAN interface to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable BBaaada Figure 1 Location of LAN interface and MAC label 1220 Infinity LC 23 2 24 Installation TCP IP parameter configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are P address Subnet Mask Default Gateway The TCP IP parameters can be configured by the following methods by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol by manually setting the parameters using Telnet The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch Configuration Switches The configuration switch can be accessed at the rear left side of the instrument The Agilent 1220 Infinity LC is shipped with switches 7 and 8 set to ON which means that the ins
111. or the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of pump components is dependent on the analytical conditions Therefore the definition of the maximum limits needs to be determined based on the specific operating conditions of the instrument The Agilent 1220 Infinity LC pump provides a series of EMF counters for the pump head Each counter increments with pump use and can be assigned a maximum limit that provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The pump provides the following EMF counters Pump liquimeter The pump liquimeter displays the total volume of solvent pumped by the pump head since the last reset of the counters The pump liquimeter can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed Seal wear counters The seal wear counters display a value derived from pressure and flow both contribute to seal wear The values increment with pump usage until the counters are reset after seal maintenance Both seal wear counters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed 138 1220 Infinity LC Preventive Maintenance and Repair 6 EMF counters for the autosampler The user settable EMF limits for the EMF counters enable the early maintenance fe
112. ormation on UV radiation 228 on cuvette holder 192 on solvents 225 initialization mode selection 25 initialization failed 107 initialization pump 41 INJECT 48 50 injecting sample 48 injection seal 161 tefzel 49 vespel 49 injection sequence 52 injection valve 51 55 56 inlet valve 144 installation delivery checklist 18 site requirements 9 installing the autosampler sample trays 58 Internet 232 1220 Infinity LC Index L Lab Advisor 137 lamp type 16 LAN Bootp amp Store 25 Bootp service 33 Bootp 25 configuration with Bootp 33 initialization mode selection 25 link configuration selection 28 manual configuration with telnet 30 manual configuration 29 storing the settings permanently 28 TCP IP parameter configuration 24 using default 27 using stored 26 leak sensor open 101 leak sensor short 102 leak 101 linearity 16 16 line power consumption 12 voltage and frequency 12 link configuration selection 28 liquimeter 138 LMD 13 LOAD 48 49 M mainpass 52 maintenance functions 165 maintenance exchanging lamps 185 using the cuvette holder 192 make before break 48 manual configuration of LAN 29 1220 Infinity LC materials in contact with mobile phase 42 42 message ADC hardware error 126 calibration failed 126 calibration lost 133 filter check failed 127 filter missing 127 grating missing 128 grating filter motor defective 128 heater current missing 129 heater failed 129 hea
113. orrect operation of the autosampler the alignment procedure must be carried out in the correct sequence and in full that is without skipping any part To align the ALS 1 2 3 Insert a 100 vial tray into the autosampler Place capped vials into positions 15 and 95 Press Start gt gt The gripper arm moves to a position above vial 15 Answer Yesto reset the correction values to their factory defaults or No to leave them as they are Use Arm Down to move the fingers of the gripper as close as possible to the top of the vial without touching Use amp 25B2 and amp 25BC for rotation and amp 25BA and amp 25C4 for movement left and right to adjust the gripper posion in the horizontal plane 7 Use Open Gripper to open the gripper fingers 8 Use Arm Down to move the gripper arm down a further 5 mm until the vial cap and the rubber of the gripper fingers are the same height Check that the vial is in the center of the gripper fingers and readjust the position if necessary step 6 10 When you are satisfied that the gripper position is correct press Continue The gripper arm moves to a position above vial 95 11 Repeat steps 6 to 9 to align the gripper at position 95 12 Press Continue On completion of the calculation the values for both X and theta are rounded to one decimal place The correction values are stored permanently in the non volatile memory of the sampler and the sampler is initialize
114. ositions the plunger at the home position Change Gripper The Change Gripper function moves the gripper to the front of the autosampler enabling easy access to the gripper release mechanism Start moves the gripper to the front of the sample tray area End repositions the gripper at the home position Arm Position Move Arm Home Park Arm Moves the gripper arm to its home position for better access and exchange of trays Secures the gripper arm to the park position behind the sampling unit Before parking the gripper arm ensure there is no vial in the gripper 1220 Infinity LC Injector Steps 1220 Infinity LC Test Functions and Calibration 4 Each movement of the sampling sequence can be done under manual control This is useful during troubleshooting where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands that move the autosampler components to predefined positions enabling the specific step to be done Table 30 Step Valve Bypass Plunger Home Needle Up Vial to Seat Needle into Sample Draw Needle Up Injector Step Commands Action Switches injection valve to the bypass position Moves the plunger to the home position Lifts the needle arm to the upper position Moves the selected vial to the s
115. ostic information from a module and writes the resuls to a file You can view the results in three tabs General The General tab shows information about the module s firmware and options in a two column table Tables The Tables tab allows you to display the contents of all available diagnostic tables for the module You click the sign to open a table or the sign to close an open table Signals The Signals tab shows the plots of the available diagnostic signals from the module The signal plots that are available are module dependent where available both short term and long term plots are displayed for a signal Displaying Module Info To display the information for a selected module 1 Select Tools from the explorer pane The Tools screen shows all available modules for the current instrument 2 In the Tools screen select the module for which you want to display information from the Module Selection pane 3 In the Tools Selection pane select Module Info 4 Click Start The module information for the selected module is displayed 1220 Infinity LC Test Functions and Calibration 4 State Info The State Info tool displays the current status of all Agilent 1220 Infinity LC modules the status is continuously updated Unless aborted the tool runs continuously for 60 minutes You click Abort to stop the tool 1220 Infinity LC 71 4 Test Functions and Calibration Solvent Delivery System 72 Pressure
116. r 39 3 40 Agilent 1220 Infinity LC Description Principles of Operation The liquid runs from the solvent reservoir through the degasser to the DCGV and from there to the inlet valve The pump assembly comprises two substantially identical plunger chamber units Both plunger chamber units comprise a ball screw drive and a pump head containing one reciprocating sapphire plunger A servo controlled variable reluctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first plunger to move at twice the speed of the second plunger The solvent enters the pump head close to the bottom limit and leaves it at its top The outer diameter of the plunger is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between The first plunger has a stroke volume in the range of 20 100 uL depending on the flow rate The microprocessor controls all flow rates in a range of 1 uL 10 mL min The inlet of the first plunger chamber unit is connected to the inlet valve which is opened or closed allowing solvent to be drawn into the first plunger pump unit The outlet of the first plunger chamber unit is connected through the outlet ball valve and the damping unit to the inlet of the second plunger chamber unit The outlet of the purge valve assembly is then connected to the chromatographic system 1220 Infini
117. r the oven to reach the first set point 40 C Aa O N Measure the temperature of the heat exchanger and enter the value in the field Wait for the oven to reach the second set point 50 C c 6 Measure the temperature of the heat exchanger and enter the value in the field 7 Click OK to save the calibration values to the oven or Cancel to abort the calibration process 1220 Infinity LC 83 4 Test Functions and Calibration Detector Cell Test 84 VWD Cell Test Description The cell test compares the intensity of the deuterium lamp measured by the sample and reference diodes unfiltered and not logarithmized when the grating is in the zero order position The resulting intensity ratio sample reference is a measure of the amount of light absorbed by the flow cell The test can be used to check for dirty or contaminated flow cell windows When the test is started the gain is set to 1 To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The test should not be performed using the micro flow cell since the reduction in light intensity will cause the test to fail Evaluating the VWD Cell Test Results The intensity ratio is dependent on the degree of contamination of the flow cell windows and on the type of flow cell used The lower the ratio the more light is absorbed by the flowcell Table 31 Probable causes of excessive flowcell absorbance Cause Corrective
118. re break passage in the stator ensures flow is not interrupted when the valve is switched between the INJECT and LOAD positions and back again Load position Inject position Figure 15 Rheodyne 6 port sample injection valve 48 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Using the Manual Injector The Injection Seal The manual injector is supplied with a PEEK injection seal as standard Injecting Sample Ejection of mobile phase When using sample loops larger than 100 pl mobile phase may be ejected from the needle port as the mobile phase in the sample loop decompresses Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Needle port Waste to column Sample loop 1220 Infinity LC LOAD Position In the LOAD position see Figure 16 on page 49 the pump is connected directly to the column ports 2 and 3 connected and the needle port is connected to the sample loop At least 2 to 3 sample loop volumes more if better precision is required of sample should be injected through the needle port to provide good precision The sample fills the loop and excess sample is expelled through the vent tube connected to port 6 from pump Figure 16 LOAD Position 49 3 Agilent 1220 Infinity LC Desc
119. re optimal performance of the instrument Power Considerations The Agilent 1220 Infinity LC power supply has wide ranging capabilities Consequently there is no voltage selector at the instrument WARNING Instrument is partially energized when switched off The power supply still uses some power even when the power switch on the front panel is turned OFF Repair work at the detector can lead to personal injuries e g shock hazard when the detector cover is opened and the instrument is connected to power To disconnect the detector from the power line unplug the power cord WARNING Incorrect line voltage to the instrument Shock hazard or damage to your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument only to the specified line voltage CAUTION In case of an emergency it must be possible to disconnect the instrument from the power line at any time Make sure that there is easy access to the power cable of the instrument so that the instrument can quickly and easily be disconnected from the line voltage Provide sufficient space next to the power socket of the instrument to allow the cable to be unplugged 1220 Infinity LC 9 1 Power Cord Different power cords are offered as options with the system The female ends of all power cords are identical The female end plugs into the power input socket at the rear left side of the
120. ription INJECT Position In the INJECT position see Figure 17 on page 50 the pump is connected to the sample loop ports 1 and 2 connected All of the sample is washed out of the loop onto the column The needle port is connected to the vent tube port 5 Needle port from pump to column Sample loop Figure 17 INJECT Position Needles CAUTION Needle can damage valve Always use the correct size needle The manual injector is not supplied with syringes or needles Use needles with 0 028 inch outer diameter 22 gauge x 2 inch long needle without electro taper and with 90 point style square tip 50 1220 Infinity LC Agilent 1220 Infinity LC Description 3 Autosampler 1220 Infinity LC Introduction to the Autosampler Three sample rack sizes are available for the autosampler The standard full size rack holds 100 x 1 8 ml vials while the two half size racks provide space for 40 x 1 8 ml vials and 15 x 6 ml vials respectively Any two half size rack trays can be installed in the autosampler simultaneously The analytical head device provides injection volumes from 0 1 100 ul The autosamplers transport mechanism uses an X Z Theta movement to optimize vial pick up and return Vials are picked up by the gripper arm and positioned below the sampling unit The gripper transport mechanism and sampling unit are driven by motors Movement is monitored by optical sensors and optical encoders
121. ror Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook 98 1220 Infinity LC Error Information 5 General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Compensation Sensor Open The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1220 Infinity LC 99 5 Error Information Compensation Sensor Short The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistan
122. s Up to 1500 pl with multiple draw hardware modification required 1220 Infinity LC Introduction 1 Table4 Performance Specifications Agilent 1220 Infinity LC Autosampler Type Specification Replicate injections 1 99 from one vial Precision lt 0 25 RSD from 5 100 ul lt 1 RSD 1 5 yl variable volume Minimum sample volume 1 ul from 5 ul sample in 100 pl microvial or 1 pl from 10 pl sample in 300 pl microvial Carryover Typically 0 1 0 05 with external needle cleaning Sample viscosity range 0 2 50 cp Sample capacity 100 x 2 ml vials in 1 tray 40 x 2 ml vials in tray 15 x 6 ml vials in tray Agilent vials only Injection cycle time Typically 50 s depending on draw speed and injection volume Performance Specifications Agilent 1220 Infinity LC Column Oven Table5 Performance Specifications Agilent 1220 Infinity LC Column Oven Type Specification Temperature range 5 degrees above ambient to 60 C Temperature stability 0 15 C constant Composition and Flow Rate Temperature accuracy 0 8 C Column capacity one 25 cm column Internal volume 6 ul 1220 Infinity LC 15 1 Introduction Performance Specifications Agilent 1220 Infinity LC VWD Table6 Performance Specifications Agilent 1220 Infinity LC VWD Type Specification Comment Detection type Light source Wavelength range Noise Drift Linearity Wavelength accuracy Band width Flow cel
123. s network all the time But on the other hand You may not have any configuration method other than Bootp If this is the case you start the Bootp Server temporarily power on the Agilent 1220 Infinity LC using the initialization mode 25 2 26 Installation Bootp amp Store wait for the Bootp cycle to be completed close the Bootp Server and power off the Agilent 1220 Infinity LC Then you select the Using Stored initialization mode and power on the Agilent 1220 Infinity LC again From now on you can establish the TCP IP connection to the instrument using the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter Figure 3 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when you want the Agilent 1220 Infinity LC to obtain its parameters from a Bootp Server each time it is powered on the recommended initialization mode is Bootp Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the Agilent 1220 Infinity LC The TCP IP connection is established using these parameters The parameters must have been configured previously by one of the described methods Non Volatil RAM Stored Parameter Figure 4 Using Stored Principle 1220 Infinity LC 1220 Infinity LC Installati
124. s required Preparations When the needle is visibly damaged When the needle is blocked inch wrench supplied in accessory kit 25mm Hex key supplied in accessory kit A pair of pliers p n Description 1 G1313 87201 Needle assembly for G1313 87101 or G1313 87103 needle seat Select Change Needle in the Tools function in the Instrument Utilities or Lab Advisor Software and select Start When the needle is positioned approximately 15 mm above the needle seat remove the upper front cover Personal injury To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not bend the safety flap away from its position or attempt to remove the safety cover gt Do not attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle 166 1220 Infinity LC Preventive Maintenance and Repair 6 Autosampler 1 Select Needle Down until the needle screw is aligned 2 Remove the sample loop fitting from the needle fitting with the hole in the safety cover NC DAD 3 Loosen the fixing screw 1 and lift out the needle 2 4 Select Needle Down to move the needle arm to its lowest position The needle arm must be in its lowest position before installing the new needle otherwise leaks at the needle seat will occur due to incorrect needle installation 1220 Infinity LC 167 Preventive Mainte
125. sage box 73 4 Test Functions and Calibration During purging the General tab shows the current channel that is being purged and the remaining purge time The Signals tab shows a plot of pressure against time for the complete purge cycle 5 When the purge time has elapsed and the request to close the purge valve appears close the purge valve on the pump then click OK to close the message box The pump purge process is complete 74 1220 Infinity LC Autosampler Maintenance Positions 1220 Infinity LC Change Needle Test Functions and Calibration 4 The Change Needle function moves the safety flap out of position and positions the needle for easy exchange and alignment Start Up Down End moves the safety flap away from the needle and positions the needle approximately 15 mm above the needle seat moves the needle arm up stepwise moves the needle arm down stepwise The lowest position is used to align the needle at the correct position in the needle arm repositions the safety flap around the needle Change Piston The Change Piston function draws the piston away from the home position relieving the tension on the spring In this position the analytical head assembly can be removed and reinstalled easily after maintenance 75 4 76 Test Functions and Calibration Start draws the piston away from the home position relieving the tension on the spring End rep
126. sembly Exchange the sample transport assembly Exchange the sample transport assembly Exchange the main board 117 5 118 Error Information Initialization Failed The autosampler failed to complete initialization correctly The autosampler initialization procedure moves the needle arm and transport assembly to their home positions in a predefined sequence During initialization the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 Defective sampling unit flex board Exchange the transport assembly 3 Defective transport assembly flex board Exchange the transport assembly 4 Defective sampling unit motor Exchange the defective sampling unit motor 5 Defective ASM board Exchange the ASM board Initialization with Vial The autosampler attempted to initialize with a vial still in the gripper During initialization the autosampler checks correct operation of the gripper by closing and opening the gripper fingers while monitoring the motor encoder If a vial is still in the gripper when initialization is started the gripper fingers cannot close causing the error message to be generated Probable cause Suggested actions 1 Vial still in gripper Remove the via
127. sion defined as the quotient of the intensity of the transmitted light I divided by the intensity of the incident light Io is the extinction coefficient which is a characteristic of a given substance under a precisely defined set of conditions of wavelength solvent temperature and other parameters is the concentration of the absorbing species usually in g l or mg l and is the path length of the cell used for the measurement Therefore flow cells with longer path lengths yield higher signals Although noise usually increases a little with increasing path length there is a gain in signal to noise ratio For example the noise increases by less than 10 but a 1220 Infinity LC Agilent 1220 Infinity LC Description 3 70 increase in signal intensity is achieved by increasing the path length from 6 mm to 10 mm When increasing the path length the cell volume usually increases in our example from 5 to 13 ul Typically this causes more peak dispersion As Figure 24 on page 65 demonstrates this does not affect the resolution in the gradient separation in our example As a rule of thumb the flow cell volume should be about 1 3 of the peak volume at half height To determine the volume of your peaks take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3 Analysis of pesticide standard 6 mm optical path length Absorbance 10 mm optical path length
128. sor short 102 eak 101 metering home failed 119 missing pressure reading 107 missing vial 120 missing wash vial 120 motor drive power 108 motor failed 121 needle down failed 122 needle up failed 123 pressure above upper limit 109 pressure below lower limit 109 pump head missing 111 pump configuration 110 remote timeout 103 safety flap missing 123 selection valve failed 110 servo restart failed 112 shut down 103 stroke length 112 synchronization lost 104 234 temperature limit exceeded 113 temperature out of range 113 timeout 104 valve to bypass failed 124 valve to mainpass failed 124 vial in gripper 125 wavelength check failed 133 error message wait timeout 116 error zero solvent counter 116 errror unknown error 2055 116 exchanging dual channel gradient valve DCGV 158 injection seal 161 passive inlet valve 144 purge valve frit 148 purge valve 148 F fan failed 100 flow cell correction factors 65 types and data 16 flow precision 14 14 flowrange 13 flow unstable 195 G general error messages 99 gradient valve DCGV 158 gradient formation 14 gripper fingers 57 gripper arm repair 181 gripper 57 gripper position verification 77 half trays 58 hexagonal key 4mm 151 152 155 157 holmium oxide declaration of conformity 231 hydraulic path 39 hydraulic system 13 l increased system pressure 195 index limit 106 index adjustment 105 index missing 106 inf
129. ss Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return 1220 Infinity LC 31 2 Installation 5 Use the and press Enter to list the current settings information about the LAN interface MAC address initialization mode Initialization mode is Using Stored TCP IP Properties actiue fiddress 134 40 27 95 active TCP IP settings 255 255 248 0 134 48 24 1 TCP IP status here ready connected to PC with controller software e g Agilent ChemStation here not connected no connections Figure 10 Telnet Current settings in Using Stored mode 6 Change the IP address in this example 134 40 27 99 and type to list current settings E C WINDOWS system32 cmd_exe telnet 134 40 27 95 gt ip 134 40 27 99 change of IP setting to gt LAN Status Page Initialization mode is Using Stored IP Address 134 40 27 25 active TCP IP settings Subnet Mask 255 255 248 0 Gateway 134 480 24 1 134 40 27 99 255 255 248 8 134 490 24 1 stored TCP IP settings in non volatile memory connected to PC with controller no connections software e g Agilent ChemStation here not connected Figure 11 Telnet Change IP settings 32 1220 Infinity LC 1220 Infinity LC Installation 2 7 When you have finished typing the configuration parameters type exit
130. st stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl 05 gt 2COCly 2HCI This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol 225 9 Appendix Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF 226 1220 Infinity LC Appendix 9 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1220 Infinity LC 227 9 Appendix UV Radiation 228 Emissions of ultraviolet radiation 200 315 nm from this product is
131. start up group and is started automatically during the boot process of the PC Open the Bootp Settings window and enter the default settings for your setup Launch the Manager The Bootp Manager screen opens showing all network hardware that has been added initially empty Click Add to enter the enter the module specific information MAC address from the label on the instrument host name IP address comment instrument name location subnet mask if different gateway if required Click OK The parameters are added to the Bootp Manager and to the TabFile Click Exit Manager and OK to exit the Agilent Bootp Service Turn on the instrument wait about 30 60 seconds and view the LogFile see Figure 13 on page 35 It should display the request from the detector with the hardware MAC address 1220 Infinity LC 1220 Infinity LC Installation 2 02 03 05 16 33 56 PM Status BOOTP Request received at outer most layer Status BOOTP Request received from hardware add ess 0030D30A0838 Status found 134 40 27 95 WADI1171 Status Host IP Address is 134 40 29 56 Status Reply to BOOTP Request has been sent Status BOOTP Request finished processing at outer most layer Figure 13 LogFile the detector has received the parameter When using this Bootp mode the parameters are not written into the non volatile memory of the detector 35 2 Installation LAN Configuration 36 1220 Infinity LC 1220 Infinity LC
132. t Always use new gaskets b Place the the window on gasket c Place the PEEK ring on the window d Insert the conical springs Make sure that the conical springs point towards the window otherwise the window might break when the cell screw is tightened e Screw the cell screw into the flow cell and tighten it 7 Repeat the procedure for the other side of the cell 8 Reconnect the inlet and outlet capillaries 9 Test the flow cell for leaks If there are no leaks insert the flow cell into the detector 10 Perform Wavelength Calibration to check the correct positioning of the flow cell Wavelength Verification Calibration on page 90 11 Replace the lower front cover 191 6 Preventive Maintenance and Repair Using the Cuvette Holder This cuvette holder can be placed instead of a flow cell in the variable wavelength detector Standard cuvettes with standards in it for example National Institute of Standards amp Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications When Tools required None Parts required p n Description 1 61314 60200 Cuvette Holder If your own standard should be used to checkout the instrument 1 Cuvette with the standard e g NIST certified holmium oxide sample 1 61313 87201 Preparations Remove the normal flow cell Have cuvette with standard available Needle assembly for G1313 87101 or G1313 87103 needle seat
133. t 1220 Infinity LC System it is highly recommended to follow the eed ges installation instructions step by step RE Agilent Technologies 2 Installation Unpacking Your System Damaged Packaging CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the Agilent 1220 Infinity LC may have been damaged during shipment Signs of damage Do not attempt to install the Agilent 1220 Infinity LC Delivery Checklist 18 Ensure all parts and materials have been delivered with the Agilent 1220 Infinity LC The delivery checklist is shown below Please report missing or damaged parts to your local Agilent Technologies sales and service office Table7 Agilent 1220 Infinity Checklist Description Quantity Agilent 1220 Infinity LC 1 Power cable 1 Flow cell Installed Instrument Utilities DVD 1 Installation guide 1 Accessory kit see below 1 1220 Infinity LC Installation 2 Accessory Kit Contents for G4286B Table8 Accessory Kit Contents for G4286B Description Part Number Quantity Accessory kit complete G4286 68755 Fitting onepiece fingertight 0100 2562 1 PTFE tubing 0 052 ID 0890 1195 5m Flexible tubing to waste 0890 1711 3m Crossover patch cable 5023 0203 1 Waste accessory kit 5062 8535 1 PTFE silicon septa 16 mm 5188 2758 1 pre slit pack of 100 Syringe 50 pL 5190 1501
134. t service representative 1220 Infinity LC 1220 Infinity LC Remote Timeout Error Information 5 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Shut Down Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation An external instrument has generated a shut down signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Suggested actions Fix the
135. t with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss
136. ta Standard Motor type 4 V 1 2 A stepper motor Seal material Vespel Tefzel available Number of ports 6 Switching time lt 150 ms 1220 Infinity LC Gripper Flex board 1220 Infinity LC Theta axis Agilent 1220 Infinity LC Description 3 Injection System Transport Assembly The transport unit comprises an X axis slide left right motion a Z axis arm up down motion and a gripper assembly rotation and vial gripping Theta motor Gripper motor Figure 22 Transport Assembly The transport assembly uses four stepper motors driven in closed loop mode for accurate positioning of the gripper assembly for sample vial transport The rotational movement of the motors is converted to linear motion X and Z axes by toothed belts connected to the drive spindles The rotation theta axes of the gripper assembly is transferred from the motor by a toothed belt and series of gears The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly The stepper motor positions are determined by the optical encoders mounted onto the stepper motor housing The encoders monitor the position of the motors continually and correct for position errors automatically e g if the 57 3 58 Agilent 1220 Infinity LC Description gripper is accidentally moved out of position when loading vials into the vial tray The initi
137. ter power at limit 130 holmium oxide test failed 130 102 102 illegal value from air inlet senso 131 ignition without cover illegal value from heater sensor 131 lamp current missing 132 lamp ignition failed 132 lamp voltage missing 133 remote timeout 103 wavelength check failed 133 metering device 55 missing pressure reading 107 motor drive power 108 multi draw option 51 N needle drive 55 55 needle type 50 needles 50 numbering of vials 58 0 operating temperature 12 operational pressure range 14 P performance specifications 14 performance specifications 16 pHrange 14 photometric accuracy 65 physical specifications humidity 12 line voltage and frequency 12 operating temperature 12 power consumption 12 safety information 12 weight and dimensions 12 piston chamber 39 piston 40 46 plateaus leak test PM 137 power considerations 9 consumption 12 cords 10 precision 49 75 81 pressure above upper limit 109 pressure below lower limit 109 pressure pulsation 45 14 43 pressure operating range 14 pressure pulsation pressure 39 preventive maintenance 137 proportioning valve high speed 39 PTFE frit 148 pump head missing 111 pump configuration 110 pump failure 195 pump head reinstalling 157 pump overview 39 pump piston 46 pump 235 Index functional principle 40 hints for successful use 46 purge valve frit 46 purge valve 148 radio interference 227 reassembling the pump
138. tp Service and open its window 4 If necessary modify the parameters for the Agilent 1220 Infinity LC according to your needs using the existing configuration 1220 Infinity LC Installation 2 5 Press OK to exit the Bootp Manager 6 Turn on the Agilent 1220 Infinity LC and view the Bootp Server window After some time the Agilent Bootp Service will display the request from the LAN interface The parameters are now stored permanently in the non volatile memory of the Compact LC 7 Close the Agilent Bootp Service and turn off the Agilent 1220 Infinity LC 8 Change the Configuration Switch settings of the Agilent 1220 Infinity LC to Using Stored mode 9 Power cycle the Agilent 1220 Infinity LC The Agilent 1220 Infinity LC can now be accessed via LAN without the Agilent Bootp Service Manual Configuration 1220 Infinity LC Manual configuration alters the set of parameters stored in the non volatile memory only of the Agilent 1220 Infinity LC it does not affect the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to activate the stored parameters if the initialization mode selection switches allow it Non Volatile RAM Stored Parameter Figure 6 Manual Configuration Principle 29 2 Installation With Telnet Whenever a TCP IP connection to the Agilent 1220 Infinity LC is possible TCP IP parameters set by any method the parameters may be altered by
139. tray positions as reference points because the tray is rectangular a two point alignment is sufficient to correct all other vial positions on the tray When the correction calculation is complete the values for both X and theta are rounded to one decimal place On completion of the alignment procedure the corrected gripper positions are stored in the module s firmware To ensure correct operation of the autosampler the alignment procedure must be carried out in the correct sequence and in full that is without skipping any part 78 1220 Infinity LC ALS Alignment Controls Test Functions and Calibration 4 Button Description Keyboard Shortcut A lt v Arm Up Arm Down Open Gripper Close Gripper Start gt gt Continue gt gt Restart Rotate the gripper by increasing theta Move the gripper horizontally to the left Move the gripper horizontally to the right Rotate the gripper by decreasing theta Lifts the gripper arm Lowers the gripper arm Opens the gripper Closes the gripper Starts the execution of the procedure Shown only at the start Jumps to the next step of the procedure Shown only during alignment Restarts the execution of the step Cursor Up Cursor Left Cursor Right Cursor Down Page Up Page Down Enter Enter 1220 Infinity LC 79 4 80 Test Functions and Calibration Running the ALS Alignment Teaching To ensure c
140. trument is set to a default fixed IP address 192 168 254 11 To configure the LAN SW1 and SW2 must be set to OFF Table 12 Factory Default Settings Initialization Init Mode Using Default switches 7 and 8 set to ON Link Configuration Speed and duplex mode determined by auto negotiation 1220 Infinity LC Table 13 Installation 2 Initialization mode selection The following initialization init modes are selectable Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default 1220 Infinity LC Bootp When the initialization mode Bootp is selected the Agilent 1220 Infinity LC tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the Agilent 1220 Infinity therefore the parameters are lost with the next power cycle of the instrument Bootp Server Figure 2 Bootp Principle Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the Agilent 1220 Infinity LC Thus after a power cycle they are still available This enables a kind of bootp once configuration of the Agilent 1220 Infinity LC Example You may not want to have a Bootp Server active in hi
141. ty LC Agilent 1220 Infinity LC Description 3 Solvent Delivery System Chamber 1 Chamber 2 Purge valve To column Inlet valve To waste From solvent bottle Seal Plunger 1 Plunger 2 Ball screw drive Gear Motor with encoder Figure 14 Principle of the pump When turned on the pump runs through an initialization procedure to determine the upper dead center of the first plunger The first plunger moves slowly upwards into the mechanical stop of chamber and from there it moves back a predetermined distance The controller stores this plunger position in memory After this initialization the pump starts operation with the set 1220 Infinity LC 41 3 42 Agilent 1220 Infinity LC Description parameters The inlet valve is opened and the down moving plunger draws solvent into the first chamber At the same time the second plunger moves upwards delivering into the system After a controller defined stroke length depending on the flow rate the drive motor is stopped and the inlet valve is closed The motor direction is reversed and moves the first plunger up until it reaches the stored upper limit and at the same time moves the second plunger downwards The sequence then starts again moving the plungers up and down between the two limits During the up movement of the first plunger the solvent in the chamber is pushed through the outlet ball valve into the second chamber The second plunger draws in half of the volume
142. uitable for vravelength calibration of these detectors within the specified vravelength accuracy of the respective detector over its vravelength range Jaoury 13 2006 P N 89550 90501 Revision E Agilent Technologies DUHEUEEUEULULLULE HUELLA DINIE EHeciveby Jan 13 2006 1220 Infinity LC 231 9 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis 232 1220 Infinity LC Index Index GLP features 13 A absorbance Beer Lambert 64 adapter 144 Agilent Technologies 232 algae growth 46 algea information 225 analytical head 55 ASTM environmental conditions 11 reference and conditions 16 AUTO mode 45 autosampler EMF counters 139 introduction 51 simple repairs 164 transprot assembly parts 165 ball screw drive 40 band width 6 5 nm 16 Beer Lambert law 64 benchspace 10 blockage 108 Bootp amp Store 25 automatic configuration 33 initialization modes 25 service 33 storing the settings permanently 28 using default 27 1220 Infinity LC using stored 26 buffer application 46 buffer solution 158 bypass 52 C caps 59 cleaning the autosampler 165 column oven 62 communications 13 compensation sensor open 99 compensation sensor short 100 composition precision 14 composition range 14 compressibility compensation 14 43 condensation 11 configuration
143. ump Head Assembly 157 Exchanging the Dual Channel Gradient Valve DCGV 158 Manual Injector 160 Overview of Maintenance Procedures 160 Flushing the Manual Injector 160 Exchanging the Injection Valve Seal 161 Autosampler 164 Introduction 164 Exchanging the Needle Assembly 166 Exchanging the Needle Seat Assembly 170 Exchanging the Rotor Seal 173 Exchanging the Metering Seal 177 Exchanging the Gripper Arm 181 Detector 184 E Agilent Technologies 135 136 Preventive Maintenance and Repair Introduction 184 Exchanging the Deuterium Lamp 185 Exchanging a Flow Cell 187 Repairing the Flow Cell 190 Using the Cuvette Holder 192 Correcting Leaks 194 Algae Growth in HPLC Systems 195 Preventive Maintenance PM is an Agilent Technologies recommended procedure designed to reduce the likelihood of electro mechanical failures Failure to perform preventive maintenance may reduce the long term reliability of your Agilent 12 20 Infinity LC 1220 Infinity LC Preventive Maintenance and Repair 6 PM Scope of Work and Checklist 1220 Infinity LC Preventive Maintenance Scope of Work and Checklist To perform a preventive maintenance PM follow the PM Scope of Work and PM Checklist step by step The PM Scope of Work and PM Checklist documents can be found in the on the DVD for the Lab Advisor Software 137 6 Preventive Maintenance and Repair Early Maintenance Feedback EMF counters for the pump The user settable EMF limits f
144. with Bootp 33 configurations 8 control system 13 correction factors for flow cells 65 counter autosampler 139 detector 139 injection valve 139 liter 138 needle movement 139 sealwear 138 cuvette holder 192 D data evaluation 13 declaration of conformity 231 delay volume 42 42 delivery checklist 18 description 48 detection type 16 detector EMF counters 139 features 63 dimensions 12 drift 16 dual plunger in series design 39 dual channel gradient valve 158 E Early maintenance feedback 13 EE 2060 110 electronic waste 230 electrostatic discharge ESD 142 EMF pump head 138 EMF counters pump 138 encoder missing 105 environment 11 error message ADC hardware error 126 error messages fan failed 100 filter check failed 127 filter missing 127 grating missing 128 error messages arm movement failed 117 calibration failed 126 compensation sensor open 99 compensation sensor short 100 233 Index encoder missing 105 grating filter motor defective 128 heater current missing 129 heater power at limit 130 heater failed 129 holmium oxide test failed 130 ignition without cover 102 102 illegal value from air inlet sensor 131 illegal value from heater sensor 131 index adjustment 105 index limit 106 index missing 106 initialization with vial 118 initialization failed 107 118 invalid vial position 119 amp current missing 132 amp ignition failed 132 amp voltage missing 133 eak sensor open 101 eak sen
145. y LC Agilent 1220 Infinity LC Description 3 Solvent Delivery System This chapter provides an overview on the operational principles of the Solvent Delivery System Pump and optional Degasser Overview The pump is based on a two channel dual plunger in series design that provides all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by one pump assembly that can generate a pressure up to 600 bar The solvents are degassed by a vacuum degasser and solvent compositions are generated on the low pressure side by a high speed proportioning valve The dual channel gradient pump includes a built in dual channel online vacuum degasser The isocratic pump of the Agilent 1220 Infinity LC has no degasser The pump assembly includes a pump head with an inlet valve and an outlet valve A damping unit is connected between the two plunger chambers A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head Degasser 1220 Infinity LC The dual channel gradient pump comes with a built in online degasser The degasser is switched on automatically when the pump is switched on even if the flow is set to 0 mL min A constant vacuum of 75 Torr 100 mbar is created in the vacuum chamber of the two channels The solvent flows through a Teflon AF tube with an internal volume of 1 5 mL channel inside the vacuum chambe
146. yringe adapter 9301 1337 1 1220 Infinity LC Installation 2 Table 10 Accessory Kit Contents for G4290B Description Part Number Quantity Solvent reservoir 1 L 9301 1420 1 Solvent reservoir amber 1 L 9301 1450 1 Bottle head assembly 61311 60003 2 Optional Tool Kit for Agilent 1220 Infinity LC Table 11 Optional Tool Kit for Agilent 1220 Infinity LC Description Part Number Quantity Tool kit complete 64296 68715 Mounting tool for flangeless 0100 1710 1 nut Wrench 1 4 inch to 5 16 inch 8710 0510 2 Wrench open 14 mm 8710 1924 1 Wrench 1 2 inch amp 9 16 inch 8720 0025 1 Seal insert tool 01018 23702 1 Hex key 4 mm 15 cm long 8710 2392 1 T handle Hex key 9 64 inch 15 cm long 8710 2394 1 T handle Hex key 3 mm 12 cm long 8710 2411 1 Hex key 2 5 mm 12 cm long 8710 2412 1 straight handle Screwdriver Pozidriv shaft 8710 0899 1 1220 Infinity LC 21 2 Installation LAN Configuration 22 To do first The Agilent 1220 Infinity LC has an on board LAN communication interface 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear left side of the Instrument next to the configuration switch Part number of the detector G4280 61000 main board Revision Code 2B ZZ 747 4 Vend
147. ysis where required 1220 Infinity LC Error Information 5 Pump Error Messages These errors are specific to the pump Encoder Missing The optical encoder on the pump motor in the module is missing or defective The processor checks the presence of the pump encoder connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Defective or disconnected pump encoder Ensure the connector is clean and seated connector correctly 2 Defective pump drive assembly Exchange the pump drive assembly Index Adjustment The encoder index position in the module is out of adjustment During initialization the first plunger is moved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals plungers and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Exchange the pump drive assembly 1220 Infinity LC 105 5 Error Information Index Limit The time required by the plunger to reach the encoder index position was too short pump During initialization the first plunger is moved to the mechan

Agilent 1220 Infinity LC System

Contents

Download Pdf Manuals

Related Search

Related Contents