Pipetman Classic Users Guide
Contents
1. oipekman EEN The World Pipetting Standard EN USER S GUIDE EN IMPORTANT NOTICE Do not lubricate this pipette F The use of lubricant cancels the S warranty of this pipette P GILSON pipetman EEE Contents 1 INTRODUCTION 2 PaRTs CheCk LIsT 3 DesCRIPTION 4 seTTING The VOLUMe 5 PIPeTTING 6 GeNeRaL GUIDeLINes fOR GOOD PIPeTTING 7 aCCess ORles 8 GLP fea TURes 9 TROUbLesh OOTING 10 Leak TesT 11 MalINTeNaNCe 12 Clea NING aND DeCONTa MINaTION 13 sPeCIfICaTIONs 14 sPaRe Pa RTs eC DeCLa RaTION Of CONFORMITY Wa RRa NTY 1 INTRODUCTION Page The Pipetting Standard Gilson s PIPETMAN Classic is designed and manufactured to provide you witha range of robust accurate and precise pipettes The PIPETMAN Classic is a fully adjustable air displace ment pipette with the selected volume shown on adigital indicator volumeter gt Eight models cover a range from 0 2 uL to 10 mL for many applications P2 and P10 measurement and transfer of micro volumes DNA sequencing and enzyme assays P20 P100 P200 P1000 measurement and transfer of general aqueous solutions acids and bases P5000 P10ml measurement and transfer of large volumes N USER S GUIDE 2 PARTS CHECK LIST Just take a moment to verify that the following items are present PIPETMAN Classic User s Guide Safety bag Certificate of conformity including bar code2. D Seal O ring 5 sets F144861 F144862 F2 Tip ejector Adapter F144879 F144879 P20 F123600 and P100 F123615 Description Service Kit 1st level F144495 F144496 Service Kit 2nd level F161972 F161973 Seal O ring 5 sets F144863 F144864 P200 F123601 and P1000 F123602 Description Service Kit 1st level F144497 F144498 Service Kit 2nd level F161974 F161978 Seal O ring 5 sets F144865 F144866 P5000 F 123603 and P10ml F161201 Description service kit 1st level f 144499 f 144503 Push button assembly f 144787 f161281 seal O ring 5 sets f 144867 f 161829 Tip holder f 123608 f161263 pipebman EER EC DECLARATION OF CONFORMITY The company Hereby certifies on its sole responsibility that the products listed below comply with the requirements of the following European Directives Annex II self declared Villiers le Bel May 2 4 2012 D fee A El Sayed H Le Dorze General Manager Quality Manager C P GILSON 20 AN USER S GUIDE WARRANTY Gilson warrants this pipette against defects in materialundernormal use and service for a period of 12 monthsfromthe date of purchase This warranty shall not apply to pipettes which are subject to abnormal use and or improper or inadequate maintenance contrary to the recommendations given in the User s guide including but not limited to pipettes which have been subjected to physical damage improper handling or spilla
3. N USER S GUIDE tissue impregnated with soap solution to remove all dirty marks If the pipette is very dirty a brush with soft plastic bristles may be used 4 Wipe the entire pipette and the tip ejector with a soft cloth or lint free tissue soaked with distilled water Refit the tip ejector and allow the pipette to dry Internal The following components only can be immersed in a cleaning solution connecting nut tip ejector tip holder piston assembly seal and O ring D Disassemble the pipette as described in the Chapter 11 Maintenance Set aside the upper part in a clean dry place Clean the individual components of the lower part of the pipette using an ultrasonic bath 20 minutes at 50 C or with a soft cloth and brushes Small round brushes with soft plastic bristles may be used toclean the interior of the tip holder 4 Rinse the individual components with distilled water Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue O Reassemble the pipette as described in the Chapter 11 Maintenance Autoclaving The upper part body and the piston assembly of the pipette are not autoclavable Only the following parts may be autoclaved tip ejector tip holderand connecting nut The O ring and seal are not autoclavable they may be cleaned or replaced with the one specified in Chapter 14 Spare Parts Clean the parts to be autoclaved especially the tip ho
4. Replace both parts Tips fall offor Low quality tips Use PIPeTMaN Dla MOND tips do not fit Damaged tip holder Replace the tip holder correctly Damaged tip ejector Replace tip ejector ejector spacer is missing Mount the spacer on the tip ejector The ejector spacer is damaged Replace the ejector spacer The tip ejector is loose assemble the tip ejector properly The ejector lock is misaligned a lign the ejector lock However if you can t solve the problem contact your Gilson representative 10 LEAK TEST This test may be performed at any time to check that the pipette does notleak especially after performing a main tenance or decontamination procedure If a pipette fails this test replace the O ring and seal After making sure thatthe pipette is correctly reassembled repeat this test For the P2 to P200 models aD Fit a PIPETMAN DIAMOND Tip Set the pipette to the maximum volume given in the specifications and pre rinse Aspirate the set volume from a beaker of distilled water Maintain the pipette in the vertical position and wait for 20 seconds Ifa water droplet appears at the end of the tip there is a leak If you see no droplet re immerse the tip below the surface of water N USER S GUIDE The water level inside the tip should remain constant if the level goes down there is a leak For the P1000 to P10ml models Fit a PIPETMAN DIAMOND tip Set the pipette to the maximum volume
5. of the pipette until it is gripped firmly by the metal tip ejector rod Changing the Tip holder no tools required MD Remove the tip ejector see above Unscrew the connecting nut by turning it counter clockwise Carefully separate the lower and upper parts Remove the piston assembly O ring and seal Clean autoclave or replace the tip holder Reassemble the pipette refer to the figure page 13 Tighten the connecting nut turn clockwise Refit the tip ejector see above Servicing the Piston Assembly You may remove the piston assembly for cleaning pur poses only Ifthe piston assembly is changed the pipette must be adjusted and calibrated in a Gilson authorized Service Center aa The piston assembly must not be autoclaved Remove the tip ejector see above 2 Unscrew the connecting nut by turning it counter clockwise Carefully separate the lower and upper parts Remove the piston assembly O ring and seal Clean and decontaminate the piston assembly O Reassemble the pipette refer to the figure page 13 IN USER S GUIDE Tighten the connecting nut turn clockwise Refit the tip ejector see above TTT Color coded push button i F Connecting nut Piston assembly gt seal tt O ring _ gt e gt Tip holder gt lt _ __ lip ejector pipetman EEN Changing the O ring The O ring and seal are o
6. sticker 3 DESCRIPTION ge Please refer to the following chapters for a full description of the different parts and functions of the pipette Color coded push button Thumbwheel Tip ejector button Connecting nut body or handle JA DlaMOND tip pipetman EEN 4 SETTING THE VOLUME The volume of liquid to be aspi P10 P2 rated is set using the volumeter 1 0 The dials are colored either 7 black or red to indicate the Li d 1 25pL 7 5 pL position of the decimal point depending on the model see P100 P200 examples 0 1 7 2 5 5 75ul 125 ul Model Color of volumeter numbers Black Red Increment P2 uL 0 01 ul 0 002 pL P5000 P10ml P10toP20 pL O1pL 0 02 pL 71 0 P100 P200 pL 02u P1000 P5000 0 01mL mL 0 002 mL P10ml mL O mL 0 02 ml a Au 1 25mL 7 5mL The volume is set by turn Pushen ing the thumbwheel or the push button The push button makes it easier and quicker to set volumes especially when wearing gloves The thumb wheel may be turned to slowly reach the required setting To obtain maximum accuracy when setting the volume pro ceed as follows gt when decreasing the volume setting slowly reach the required setting making sure not to overshoot the mark gt whenincreasing the volume setting pass the required value by 1 3 ofa turn and then slowly decrease to reach the volume making sure not to overshoot the mark N USER S GUIDE 5 PIPE
7. TTING For optimum performance use of PIPETMAN DIAMOND Tips with your PIPETMAN Classic is strongly recommended These tips made from pure polypropylene have the Gilson logo engraved on their collar ensuring that you have a genuine Gilson product Plastic tips are for a single application they must not be cleaned for reuse USS PIPETMAN Classic can also be used with the main tip brands Fitting the tips To fita new PIPETMAN DIAMONDTip push the tip holder into the tip using a slight twisting motion to ensure a firm and airtight seal ES forthe P2 andP10 models a dual position adapter plastic is required to fit DL10 tips long tips or D10 tips short tips The metallic rod of the tip ejector is shaped so that the adapter may be clipped to it in either position P2 and P10 models are delivered with the adapter in place positioned in the longer slot ready to use DL10 tips When D10 tips which are shorter are used the adapter must be repositioned in the shorter slot as follows Pull the adapter down from the metallic rod Turn the adapter through 180 Refit the adapter so that the end of the metallic rod engages the shorter slot of the adapter Short tips Long tips Dual position adapter Pre rinse the tips for P2 and P10 Some liquids e g protein containing solutions and organic solvents can leave a film of liquid on the inside wall of the tip pre rinse the tip to minimize any errors that may
8. be related to this phenomenon Pre rinsing consists of aspirating the first volume of liquid and then dispensing it back to waste Subsequent volumes that you pipette will have levels of accuracy and precision within specifications pipetman EEN Aspirate D Press the push button to the first stop this corresponds to the set volume of liquid 2 Hold the pipette vertically and immersethetip inthe liquid see immersion depth table page 7 Release the push button slowly and smoothly to top posi tion to aspirate the set volume of liquid Wait one second time depends on model see table then withdraw the pipette tip from the liquid You may wipe any droplets away from the outside of the tip using a medical wipe however if you do so take care to avoid touching the tip s orifice First stop Second stop Dispense Place the end of the tip against the inside wall of the recipient vessel at an angle of 10 to 40 2 Press the push button slowly and smoothly to the first stop Wait for at least a second then press the push button to the second stop to expel any residual liquid from the tip Keep the push button pressed fully down and while removing the pipette draw the tip along the inside surface of the vessel N USER S GUIDE 4 Release the push button smoothly Eject the tip by pressing firmly on the tip ejector button 6 GENERALGUIDELINES FOR GOOD PIPETTING Makesurethat
9. e lifetime of the piston see Chapter 7 Accessories Do not pipette liquids having temperatures above 70 C or below 4 C The pipette can be used between 4 C and 40 C but the specifications may vary according to the temperature refer to the ISO 8655 2 standard for conditions of use 7 ACCESSORIES To make pipetting more comfortable and more secure Gilson has developed several accessories To avoid the possibility of liquid running back into the pipette store the pipette vertically CaRROUseL Pipette stand 7 pipettes f161401 sINGLe pipette holder f161406 To identify or personalize your pipette COLORIS clips are available COLORIs clips mixed colors set of 10 f 161301 COLORIs clips yellow set of 10 f 161303 COLORIs clips blue set of 10 f 161305 WithTHE JIMMY hands free microtube opener you can open both snap cap and screw cap microtubes 4 To protect the piston when pipetting corrosive liquids you can use a specific tip holder and filter for the model P1000 8 GLP FEATURES The SerialNumberis engraved on the body of the pipette It provides unique identification of your pipette and the date of manufacture Ex AA 10369 The Bar Code on the box and the certificate of conformity provide traceability of your pipette 9 TROUBLESHOOTING N USER S GUIDE A quick inspection of the pipette may help you to detect a problem 33 gt Youmayd
10. en genuine PIPETMAN DIAMOND Tips are used Each pipette is inspected and validated by qualified technicians in accordance with the Gilson Quality System Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 standard by type testing The adjustment is carried out under strictly defined and monitored conditions ISO 8655 6 16 N USER S GUIDE Gilson Maximum Permissible Errors Single channel Maximum Permissible Errors Model Volume Gilson ISO 8655 Reference ul systematic Random systematic Random error ul error uL error ul error ul P2 f 144801 D10 DL10 P20 f123600 D200 lt 0 10 lt 0 10 lt 0 10 lt 0 10 P200 f123601 D200 D300 P5000 f 123603 D5000 Heo Ie Re Wo aaa A am N N The data given in the tables conform to the ISO 8655 2 Standard With a precise pipetting technique see Chapter 6 General guidelines for good pipetting the P2 model may be used to aspirate volumes as low as 0 1 uL and the P10 model as low as 0 5 uL oipeLman EEN 14 SPARE PARTS Service Kit 1st level includes 3 piston seals 3 O rings _1tip holder Service Kit 2ndlevel includes 1 push button O 1 connecting nut B 1 tip ejector Q oronly forthe P2andP10models 1 tip ejector and 1 adapter IN USER S GUIDE Description Service Kit 1st level F144501 F144502 A B F1 F2 Service Kit 2nd level F161970 F161971 C
11. ge or exposure to any corrosive environment This warranty shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates Gilson s sole liability under this warranty shall be limited to at Gilson s sole option repair or replacement of any defective components of pipettes or refund of the purchase price paid for such pipettes THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESS OR IMPLIED INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL PUNITIVE INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR IMPLIED WARRANTY 21 cassie W 22 23 LT801120 C 2012 Gilson SAS All rights reserved May 2012 English Printed in France Specifications subject to change without notifications errors omitted www gilson com sales gilson com service gilson com training gilson com Gilson Inc World Headquarters 3000 Parmenter Street PO Box 620027 Middleton WI 53562 0027 USA Tel 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 Gilson S A S 19 avenue des Entrepreneurs BP 145 F 95400 Villiers le Bel France Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 P GILSON
12. given in the specifications Aspirate the set volume from a beaker of distilled water Maintain the pipette in the vertical position and wait for 20 seconds If a water droplet appears at the end of the tip there is a leak 11 MAINTENANCE Routine maintenance will help keep your pipette in good condition ensuring a continued high level of performance Maintenance is limited to cleaning or au toclaving the parts specified under Chapter 12 Cleaning and Decontamination or to replacing the push button connecting nut tip ejector tip holder seal and O ring PIPETMAN P2 and P10 should not be disassembled so you may only replace the push button tip ejector dual position tip ejector and its adapter With these pipettes if the tip holder is damaged the piston may also be damaged After replacing any parts you should verify the performance of your pipette following the verification procedure available on the Gilson website www gilson com If the pipette needs to be readjusted please contact your local Gilson authorized Service Center pipetman EEN Changing the Tip ejector Toremove the tip ejector keep the tip ejector button depressed and pull down on the flanged upper part of the tip ejector with the other hand To refit the tip ejector keep the tip ejector button depressed slide the end of the tip ejector over the end of the tip holder and push the plastic end of the tip ejector back into the body
13. lder Put the parts in an autoclaving sack Autoclave for 20 minutes at 121 C 0 1 MPa Check that the parts are dry before re assembling the pipette Set the pipette aside to stabilizeat room temperature 15 pipetman EEN Chemical Decontamination You may choose to decontaminate your pipette chemi cally in accordance with your own procedures Whatever decontaminant you use check with the supplier of the decontaminant that it is compatible with stainless steel and the plastics used in the construction of the pipette PA Polyamide PBT Polybutylene Terephtalate PC Polycarbonate PC PBT Polycarbonate Polybutylene Terephtalate POM Polyoxymethylene or PVDF Poly vinylidene Fluoride Upper Part handle ED Wipe the upper part handle of the pipette with a soft cloth or lint free tissue impregnated with the chosen decontaminant Wipe the upper part of the pipette with a soft cloth or lint free tissue soaked with distilled water or sterile water Lower Part Volumetric module The following components only can be immersed in a decontaminant solution connecting nut tip ejector tip holder piston assembly seal and O ring 13 SPECIFICATIONS PIPETMAN Classic is a high quality pipette that offers excellent accuracy and precision The figures given in the Gilson Maximum Permissible Errors table page 17 were obtained using PIPETMAN DIAMOND Tips These figures are guaranteed only wh
14. n the piston they must not be autoclaved if worn or damaged in any way chemical or mechanical they must be replaced The dimensions of the O ring vary depending on the model of pipette CD Remove the tip ejector see above Unscrew the connecting nut by turning it counter clockwise Carefully separate the lower and upper parts 4 Remove the piston assembly O ring and seal Clean or replace the seal then the O ring O Reassemble the pipette refer to the figure page 13 Tighten the connecting nut turn clockwise Refit the tip ejector see above 12 CLEANING AND DECONTAMINATION PIPETMAN Classic is designed so that the parts normally in contact with liquid contaminants can easily be cleaned and decontaminated However because the models P2 and P10 contain miniaturized parts it is best not to disassemble these pipettes yourself please contact your local Gilson authorized Service Center 35 gt You may refer to the decontamination procedure available on the Gilson website www gilson com Liquid must never enter the upper part handle of any pipette Cleaning The pipette must be cleaned as described below before it is decontaminated Soap solution is recommended for cleaning PIPETMAN Classic External D Remove the tip ejector Wipe the tip ejector with a soft cloth or lint free tissue impregnated with soap solution Wipe the entire pipette with a soft cloth or lint free 14
15. ownloadfrom the Gilson website www gilson com the 2 minute inspection which shows how to perform a quick diagnosis of your pipette Before returning any pipette to your local Gilson Service Center ensure that it is completely free of chemical biological or radioactive contamination Refer to Chapter 12 Cleaning and Decontamination Please use the included safety bagtoreturn the pipette to yourlocalGilson Service Center The following table may help you to identify and correct the problem you might encounter Symptom Possible Cause Pipette is Damaged tip holder leaking sample Worn O ring or seal Pipette won t Worn O ring aspirate Damaged tip holder Connecting nut is loose Damaged or corroded piston Improper repair or assembly Action Replace the tip holder Replace both parts Replace both parts Replace the tip holder Tighten connecting nut Return pipette to supplier see Chapter 11 Maintenance pipetman EEE Symptom Possible Cause Action Pipette is Improper repair or assembly see Chapter 11 Maintenance inaccurate Unscrew tip holder Tighten connecting nut Connecting nut is loose Tighten connecting nut Pipette is Tip holder is loose Tighten connecting nut not precise Connecting nut is loose Tighten connecting nut Incorrect operator technique Operator training Damaged or corroded piston Return pipette to supplier Damaged tip holder Replace the tip holder Worn O ring or seal
16. youoperate Table Immersion Depth the push button slowly and Wait Time and smoothly Model Immersion Wait Time Depth mm seconds When aspirating keep the tip at a constant depth below the surface of the liquid refer to the table Change the tip before as pirating a different liquid sample or reagent 4 Change the tip if a droplet remains at the end of the tip from the previous pipetting operation Each new tip should be pre rinsed with the liquid to be pipetted O Liquid should never enter the tip holder to prevent this press and release the push button slowly and smoothly never turn the pipette upside down never lay the pipette on its side when there is liquid in the tip If you use the same tip with a higher volume pre rinse the tip For volatile solvents you should saturate the air cushion ofyour pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample When the temperature of the liquid is different from the ambient temperature pre rinse the tip several times before use 10 You may remove the tip ejector see Chapter 11 Maintenance to aspirate from very narrow tubes After pipetting acids or other corrosive liquids that emit vapors remove the tip holder rinse the piston 7 oipeLman BEER O ring and seal with distilled water For the model P1000 by using a specific tip holder equipped with a filter you can increase th
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