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1. O 0 0 lt CO 0 o O D lon Source lon Source Block Description Cat No Quantity Description Cat No Quantity lon source assembly complete 109850 0250 Filament 120320 0030 Magnet TSQ Quantum GC XLS 1870001 98195 1 Filament spacer 70111221 1 Magnet ee 70001 98195 Filament retainer clip 70111 22071 _ Magnet support sss 1R119850 0710 5 lon source heater board 70111 60780 1 E Sowcespacerassemby _ 119800045 1 Source block 70111 60791 1 5 ee 76485 0032 O Source alignment studs 70111 22056 1 _ Lens 1 TSQ Quantum 19650 04104 10111 22070 1 ss Lens 2 TSQ Quantum GC 119650 0416 1 Lens retainer screw 70111 22060 1 tens 3 TSQQuantumGC 11965004223 1 _ Lens housing 119690 04200 Inlet Valve Lens insulator _ 19650 04258 lon volume El stainless steel 70111 22036 1 Balvalveseal replacement kit 16461 2002 1 lon volume 7011 22037 1 Iplet valve seal replacementkit 1192650003 1 lon volume closed exit CEI stainless steel lon volume holder stainless steel 70001 20532 1 Mechanical Rotary Vane Pump lon volume closed exit CEI 10111 22202 1 Description Cat No Quantity ees rotary vane pump 1L A0301 15101 1 lon volume closed exit inert 702. 4 Silver seal for detector base 29037000 _ Ds 5 Terminal fitting for detector base 347054586 11111 T S 8 x 1 retaining nut for detector base 35002134 EN d 5 i Threaded locking ring 35001468 1 E 4 Q D UltraFast Module Option UFM PTV FID Configuration TRACE GC Ultra UltraFast Module Option UFM S SL FID 3 Item Description Cat No Quantity Configuration TRACE GC Ultra E sss Injector guide ss 24104482 M 2 2 Siver seal for detector base 29037000 0 Terminal fitting for detector base 34705457 _ Net lt gt 2 2 es M8 x 1 PTV retaining nut for detector base 33002130 L7 DCL 1 A E 5 Supporting disk 36812760 1 emm lt gt i UltraFast Module Option UFM PTV FID Configuration TRACE GC Ultra 3 146 TriPlus GC Autosampler e Flexible sampling solutions for liquid headspace and SPME e Quick installation for easy start up Double productivity with simultaneous operation of two different Thermo Scientific GC or GC MS systems The Thermo Scientific TriPlus autosampler is the evolution of autosamplers based on three dimensional XYZ space movements of the syringe holder Besides conventional injection of liquid or headspace this platform delivers unique pluses conceived to increase your lab s efficiency and productivity TM e Snap on interchangeability of liquid headspace
3. Isolate the detector from the electronics If the spiking disappears clean the jet and the collector Increase the FID temperature to at least 150 C ww thermoscientific com chromatography C C a B 5 2 gt CD v o A V V D C2 D CD S 3 15 GC Troubleshooting continued oymptom Cause Recommended Solutions Peak Related Problems Peaks Broadening Column flow too high Reduce the flow to slightly above optimum The sample is overloading the column Reduce the amount and or concentration of the sample cn 0 0 D O O lt C 0 o O O D Double Peaks Injection speed too low Inject more rapidly in a smooth Wrong autosampler injection speed or mode Use a higher speed Peak Fronting Column or detector overloaded Decrease the injected amount Decrease the analyte concentrations Increase the split ratio ssn Column temperature too low lmereasethetemperature ssn E Stationary phase too thin Useathikerflmcolum s Poor injection technique Repeat with better injection technique Ghost Peaks Contaminated carrier gas sss Replace the cylinder Replace the filter see page 3 080 C m Contamination from laboratory glassware Ensure the glassware is clean and contamination free Decomposition of injected
4. Baseline Rising Air is leaking into the system Trace and repair the leak Baseline Rising Column contaminated Recondition the column Under Temperature Program Control Baseline High otanding Current Carrier gas flow rate too high Reduce the carrier gas flow Check the oven temperature ensuring that it doesn t exceed the column upper limit Recondition the column Replace the column Loose connections Ensure that all interconnections and screw connections are tight Baseline Irregular Detector contaminated Bake out the detector Clean the detector shape Dip After Solvent Peak Baseline Irregular shape S shaped Baseline High Frequency Noise Excessive column bleed during column temperature programming Oxygen contamination is decomposing the stationary phase Contaminated detector Reduce the upper column temperature Bake out the column Install a high temperature column Install oxygen filters in the carrier gas line Check the pneumatic and inlet systems for leaks Use correct gas purity with low oxygen content Isolate the detector from the electronics If noise disappears clean the collector Baseline Spiking Detector temperature higher than column maximum temperature Loose column fittings Column too close to flame when using an FID FID temperature too low when using an FID Tighten fittings accordingly Lower the column to the correct position 2 3mm below the tip of the Jet
5. C Tailing Column oven temperature too low Increase the column oven temperature Do not exceed the recommended maximum temperature for the stationary phase Wrong stationary phase Replace the column according to the column manufacturer s literature Incorrect column position in inlet Reinstall the column solvent Peak Tailing septum purge flow too low and or split splitless vent flow too low Too large injection size Reduce the injection size Thermo Scientific Chromatography Columns and Consumables 2012 2013 3 158 oymptom Cause Recommended Solutions Unresolved Carrier gas flow rate too high Reduce the carrier gas flow rate uum Column deteriorated Replace the column s Injection technique is not adequate Choose a correct injection technique Discrete Bleed from the column ccs Condition or change the column sse High intensity Bleed from the septum Replacetheseptum s E TRE Sample vial septa are contaminating the sample Discard sample Store samples upright in a refrigerator Use Teflon faced eaks septa with the Teflon facing downwards i e towards the sample Results Related Problems Low Concentration not compatible with the dynamic Reproducibility range ofthe detection system of Peak Area Ensure that the sample concentration is suitable for the detection system Evaluate the sample preparation sequences Compare the results with a series of standard
6. 1 Cooled Heated tray holder configurable in field as primary 24010190 n OG Primary cooled heated tray positions 1 36 for 1 2 2 omL vials 24010195 7 Primary cooled heated tray positions 1 33 for 10mL vials 24010200 D 1 Secondary cooled heated tray positions 97 192 for 94010215 1 12 25mLVas d secondary cooled heated tray positions 34 66 for 10mL vials 24010220 1 Washing Stations Description Cat No Quantity E Standard 5 10mL washing station 1905080 1 _2X100mLwashingstation sme 19050400 MM Fast washing station 19050401 1 Washing Stations ww thermoscientific com chromatography 3 147 O 0 0 lt v o o O D Thermo Scientific Chromatography Columns and Consumables 2012 2013 3 148 Turrets and Oven Description Turret AS HS incubation oven agitator SPME Description SPME fiber holder kit only for TriPlus with turret s n above 20066157 SPME fiber conditioning station only for TriPlus s n above 20066529 Septum Caps Description septum cap for SSL suitable for HS and SPME septum cap for Merlin Valve SSL PTV Bar Code Reader Description Bar code reader Labels for bar code reader Cat No 25401050 35408070 Cat No 19050434 19050502 Cat No 34750004 35001056 Cat No 19050490 38706782 Quantity Quantity SPM
7. 1 _ E lon volume key thumbscrew 119650 0206 1 Columnmeasuingtol 119640 0550 1 Heater RTD Spring 96000 20176 1 5 volume tool 19270 0001 1 _ Heater RTD spring thumbscrew 119650 0208 Front ferrule 1 8in brass A0101 08500 1 Test mix octafluoronapthalene and decafluorobenzophenone DFBZ Transfer line ferrule 1 16in to 0 4mm A0101 18100 1 graphite vespel Polaris Q External lonization lon Trap GC MS e Stop matrix interference with the power of MS e Qutstanding sensitivity in full scan mode Reliability robustness and library searchable data from external ionization source The Thermo Scientific PolarisQ is a high quality benchtop ion trap GC MS system with El CI Negative Cl MS MS and direct sample probe capability Proprietary Pulsed Positive lon Negative lon Chemical lonization PPINICI and variable buffer gas options enhance ppb sensitivity and performance in the toughest sample matrices External ionization ensures data integrity while providing robust reliable performance lon Source Description Cat No Quantity E Clionvolume su 119650 0230 1 ___ EI ion volume 119650 0220 7 El Cl combo ion volume 9650 0240 _ Flamen s 11201 60287 Filament spacer 119650 0235 96000 60087 _ lon volume holder 70001 20532 1 Mechanical Rotary Vane Pump Description Cat No Quantity Oil rotary vane pump 1L A0301 151
8. Flame Photometric Detector FPD TRACE GC Ultra Item Description Cat No Quantity QO i a A 2A Graphiteseal ssn 19004890 2 _ o EN D RR Dessus ml o o 2C 19004589 1 B gt voL 1900588 i e 2E Viton O ring 19004589 1 S VN EIER MEER C ES p 5 FPD deactivated Silcosteel jet 40404512 _ NEM 6 FangeandrelevantViton O ring 388142385 T sss EE 7 Interferentialflterforsulphur 28107000 8 4 ones o 7 _ Interferential filter for tin 28107001 1 TRACE GC Ultra oz i pm OM neo mem 3 Heater sensor coil cable 23043479898 1 10 fixing tool 20502150 1 o ee A Maintenance kit for FPD Detector Base Body TRACE GC Ultra and FOCUS GC Item Description Cat No Quantity 1 Adapter for flow measurement 35004107 1 2_ Brassblindcap 35001 1 Detector Base Body 3 Silicon seal 29033037 10 TRACE GC Ultra and 40401800 2 MEE 2903010 0 E 6 dapterforcapillarycolumn 3472543868 2 i 7 Graphite ferrule See page 3 091 mA o nes 1 uM UltraFast Module Option UFM S SL FID Configuration TRACE GC Ultra Item Description Cat No Quantity Silver seal for injector base 290336298 10 z 2 Terminal fitting for capillary column 34705458 1 Tope S 3 Wain retaining nut for injector base 35002132 1 gt
9. any solvents used and have the appropriate Material Safety Data Sheets MSDSs readily available All electrically powered instruments should be shut down and unplugged before starting Eye protection should also be worn The following table lists common GC problems encountered the possible causes and solutions for your quick reference oymptom Cause Recommended Solutions Baseline Related Problems Baseline Drifting Accumulation of stationary phase Remove the end section of the column Accumulation of impurities in the column Check impurity levels in the gas source Use correct gas purity Replace or install appropriate Gas Filters see page 3 080 Perform a leak test Check the tightness of the connections on the carrier gas line Column is baking out Allow enough time for the column to stabilize Purge valve left closed during acquisition Alter the GC program See your GC user manual for details Baseline Falling Carrier gas leak in the system Baseline Falling i NN Inadequate purge flow rate enna Increase the purge flow ME a High Purge valve left closed for too long ohorten the purge time Initial Value ani Nm o VNPT TENOR oM vae dnb AM vcr Pre filters are dirty when using a quadrupole Contact your service representative MS detector Accumulation of impurities in the column Check impurity levels in the gas source Use correct gas purity Replace or install appropriate Gas Filters see page 3 080
10. injections Check and replace the syringe at regular intervals Check and replace septum at regular intervals Poor split flow or ratio control Monitor the flow Replace the in line filter Carrier gas flow rate too low Increase the carrier gas flow rate Locate and remove possible with obstructions in the carrier gas line Check the injector column ferrules Poor Sensitivity Increased Retention Time Poor Sensitivity with Normal Retention Time Oven or injector parameters are not optimized Adjust the oven parameters Adjust the injector parameters O C O D j D gt CD V 0 x A V D C2 r CD S Retention Times Decreasing Retention Times Column is in poor condition or wrong column type used Stationary phase deteriorated by oxygen and or water Stationary phase loss due to column bleeding Increasing carrier leakage Condition the columns Change the column Use a carrier gas free of oxygen and water Replace or install appropriate gas filters see page 3 080 to 3 082 Reduce the column temperature Check the septum and column connections Increasing Carrier gas supply running out Replace the bottle Low Drifting or unstable pneumatic controller Monitor the column pressure or flow Reproducibility of Check and replace the controller if necessary Retention Times Unstable column temperature Check the main oven do
11. sample Decrease the injection port temperature Use the on column injection technique Dirty injection solution Carry out adequate clean up of sample prior to injection Broad Ghost Contaminated inlet or pneumatics Remove the column and bake out the inlet Use a high quality septum Peaks Replace the split vent filter Install an in line filter between the pneumatics and the inlet Incomplete elution of previous sample Increase the final oven program temperature or total run time Increase the column flow rate Increase the initial oven temperature Reduce the injection volume On column Install a retention gap On column Carrier gas flow too high Reduce the carrier gas flow rate Irregular Chair oolvent flooding of column shaped Peaks No Peaks After solvent Peak Incorrect column position in S SL injector too high Check the column position No Peaks at All Clogged syringe needle sss Replace or repair the syringe sss Column broken or disconnected Checkthecolumnandconnections s Defective electrometer or amplifier Check electrometer or amplifier and associated connections Replace if required Defective recording device h Replace the recording device FID flame is out Clean FID jet check detector gas flows and re light flame s no a Sample Peak Column degradation causing activity Inject a test mixture and evaluate the column
12. 01 1 C C a B 5 D gt O CD ON O A V V G zi CD CD gt eb D S 2 Inlet Valve Upgrade Option Description Cat No Quantity Ball valve seal replacement 7646120027 1 Inlet valve seal replacement kit 119265 0003 1 Accessories Description Cat No Quantity len wrench kit metri 3481201007 luminum oxide 000 60340 __ MM Back ferrule 1 8in brass 0101 02500 1 Calibration compound 5001030005 1 Filament c 19101 60287 Front ferrule 1 8in brass 8500 ess Test mix 100ng uL decafluorobenzophenone benzophenone A0301 23057 1 methylstearate Test mix 100pg uL 10pg uL and 1pg uL 96000 98044 1 Qecafluorobenzophenone Transfer line ferrule 1 16in to 0 4mm graphite Vespel A0101 18100 1 ww thermoscientific com chromatography 3 153 TSQ Quantum GC and TSQ Quantum GC XLS Triple Quadrupole GC MS MS e Higher compound sensitivity More matrix selectivity More productivity The Thermo Scientific TSQ Quantum GC is the latest evolution in triple quadrupole GC MS MS The TSQ Quantum GC provides unprecedented sensitivity for target compound analysis in complex samples Timed selected reaction monitoring t SRM unique to the 50 Quantum GC detects compounds at the expected elution time with optimum sensitivity independent of the total number of compounds a feature no other GC triple quadrupole MS can provide
13. 1082100 n _ DIP gold crucibles 0574200 1 _ lon volume for exchange CI operation 1082200 DIP HT quartz crucibles 1006070 r 0 lon volume for exchange ACE operation 1082250 1 mU _ Crucible holder 0568770 lon volume for exchange FAB operation 1082240 T_T wire units 0744860 SEV Unit electron multiplier for E DES MATES 800 1 DIP aluminum crucibles for solid samples 0568761 200 _ E Septum 12 5 PTFE coated 055331 1 __ Source heater AI 1 Ferrule Vespel 1 16 transfer line 0492490 1 ww thermoscientific com chromatography 3 155 GC Technical Information GC Method Selection and Optimization The following flow chart briefly describes the common steps in GC method development and optimization Review Preliminary method applications library development Validate anaytes _ applications index PP TraceGOLD TG 5MS N po O 0 0 lt O C CO 0 C o O t Thermo Scientific Chromatography Columns and Consumables 2012 2013 3 156 Improve sensitivity Increase injection volume Reduce film thickness Produces sharper peaks Reduce column diameter Reduces HETP and increases pressure to maintain linear flow rate Yes Validate Method optimization Increase retention Reduce temperature ramp rate Increases the distribution co efficient and increases analyte dwell time in the stationary phase Reduce ca
14. 111 22147 lon volume holder inert 70001 205327 Accessories E Lens 1 TSQ Quantum GC XL5 18119650 3414 or 102 180 Quantum 6CXLS 5 070 1R119650 3416 ___ TACNO Lens 3 TSQQuantumGCXLS 188119650 3423 EU uL NONE d uem s GC source Q00 Heather XLS TSQ 70111 60840 sss Ball Plunger 5 40 modified 10111 22000 RM UE EE JN LS tor en ais T Bin Dios 2 Assy Lens 1 2 3 GC Source 10111 60842 EEE NY Heated XLS TSQ OC MERE Lou C E Lens Circlip lon Trap DSQ 119650 0428 Calibration compound FC 43 50010 30059 _ IN Lens 1 2 3 Holder Source XLS TSQ 70111 22197 _ 40101 18100 Quad Spacer Ceramic Heated anann o0 ula or n M E 00 XLS TSQ RE Electron multiplier with flange 70001 98175 1 5 E D m Mee _ n E Ceramic Heather PCB Assy XLS TSQ 70111 60848 1 e E o e 3 E 3 154 DFS High Resolution GC MS e Ultra high sensitivity analysis of dioxins BFRs and other POPs e Highest sample throughput at lowest LOQs Highest reliability of analytical results The Thermo Scientific DFS is the highest performance highest resolution GC MS ever built for target compound analysis and structural elucidation and the first completely new instrument of its cla
15. 12 2013 3 152 ITQ Series External lonization GC lon Trap MS Excellent sensitivity in full scan operation e Advance to the power of MS for incredible selectivity in the dirtiest of matrices e External ionization source for maximum productivity reliability and classical library searchable spectra The Thermo Scientific ITQ Series is designed with the ability to upgrade The ITQ Series offers a range of operating modes from full scan MS in mind protecting your investment by adapting to your lab s changing and MS MS MS to positive and negative chemical ionization Dual work flows and needs over time If your needs change upgrade your modes for sequential full scan and MS MS or positive ion negative ion instrument to gain access to new features greater flexibility more chemical ionization PPINICI allow you to acquire both types of data power Better yet regardless of your choice you will have the most in a single injection Variable damping gas an option available exclusively sensitive GC ion trap mass spectrometer available giving you lower on the ITQ Series further improves GC MS sensitivity up to 5X or more detection limits even in matrix across a broad range of real world samples Data Dependent scanning allows you to quickly collect data confirm the identity of compounds and further reduce sample cleanup costs lon Source Spare Parts lon Trap Spare Parts Description Cat No Quantity Description Cat No Quanti
16. 150 DSQ Series Single Quadrupole GC MS Systems e Sensitivity and large dynamic range for demanding analyses e Proven performance for difficult samples e Analytical flexibility for applications from research to routine The Thermo Scientific 050 Series of single quadrupole GC MS systems includes the DSQ and DSQ II mass spectrometers The 050 Series mass spectrometers are available with either the TRACE GC Ultra gas chromatograph or the compact FOCUS GC Featuring an innovative curved prefilter for reduction of neutral noise resulting in excellent sensitivity and lower detection limits the DSQ Series of single quadrupole GC MS products are suitable for a wide variety of applications G O o CE 3 ED 60 ED O gt O CD 6 CD A V V z CD 5 CD S je z i E z DSO II Spare Parts DSO Spare Parts Description Cat No Quantity Description Cat No Quantity Magnet yoke 119850 0710 1 lon source assembly complete 119850 0250 1 MM C CIT 5 E o MEE 1 2 NE E auc e Maget 19650 02200 1 Magetsupot 99850 0710 Jon volume 19650 02380 1 Source 19800 0475 _ Pu E aa EU ee M io ee Flament spaver insulator 100259 1 m MEN M NUN CC M CU wes 1777 puc m RE ee E Du M TC c s c MM c E 4 ene a ee a T 1 l
17. 20 minutes purging air content sss a 2 lise the temperature to 100 C at 5 C min Raise the temperature to 100 C at S C min Hold for 30 minutes rra Hold for 30 minutes rcs Raise to 150 C at 5 C min Raise to 150 C at 5 C min Oe s 10 20 s Raise to 250 C at 5 C s Hold Tor 40 minutes Hold Tor 40 minutes sasnmnminnininniniininininnninintinnn v ooo LL faise to 250 C at 5 C min Ral to 300 C atS C min Hold for 40 minutes s Hold for 40 minutes ss Se 10 280 Cat S 8196 10 380 C at 11 Hold for 30 minutes Hold for 30 minutes Although quite a long procedure it will result in longer lifetimes and lower bleed for your column Performance Recovery The performance of the column may exhibit signs of deterioration end will not lower the separation effectiveness by the same ratio as over time as a result of many different causes Some of these such 50cm column length A last resort in column regeneration is column as contamination by high boiling or strongly retained compounds washing Column washing uses a pressurized vessel to force solvent can be cleared by repeating the column conditioning until a stable through the column in a reverse direction The selection of the solvent baseline is achieved is dependent on the nature of the samples that have been analyzed Other contamination such as non volatile compounds pieces of septa and t
18. E Fiber Conditioning Station Bar Code Reader AI AS 3000 and AI AS 3000 Series Il Autosamplers e Automated sampling systems for all Thermo Scientific GC and GC MS systems e Performance and simplicity in liquid sampling e Tool Free upgradability from 8 to 105 vial positions Engineered to meet the highest level requirements of ruggedness and ease of use the 3000 Series Il automatic sampling system is ideal for high throughput and QA QC control on the Thermo Scientific GC and GC MS product portfolio Utmost precision and ease of use of the plug and inject concept are combined in two available configurations 8 position single module system for small batches of samples 105 position tray system for high sample throughput Boost productivity further with the Gemini configuration which allows two autosamplers to be mounted on the same GC serving two injection ports simultaneously AIAS 3000 and Al AS 3000 Series Il Autosamplers Description Cat No Quantity __ Waste bottle complete with septum and Setof 5 n 19004646 i Tray 8 position holder sss 24010140 MEM Tray 105 position assembly 25117445 1 _ Centering Plate for Merlin Adapter S SL injector 36812734 1 _ onpi Ona sin RA p m TM IM ponite Q P O a 5 9 Co D Q gt O O CD Co Cn m CD Cn V V a ES 90 zi CD feb CD c AS 3000 Il 105 vial Autosampler ww thermoscientific com c
19. Injection Method The identification of the most appropriate injection method relies on the sample type and the boiling f point to be used in the separation The diagram below summarizes this selection process High b p PTV split injection Thermally stable Cn Low b p Split E High E High b p PTV or on column Q Thermally unstable Low b p PTV or on column High b p Low b p Low High b p Low b p On column or spli Large volume splitless e ev N e a E 2 e e E gt lt e pes c 4 o o per N E pen 3 162 Column Conditioning All Columns Except TRACE TR 1MS and TR WaxMS It is recommended that before the column is subjected to any thermal 1 Heat the column from 50 to the maximum operating temperature at gradients all oxygen has been removed because the presence of oxygen 5 C min and hold for one hour The maximum operating temperatures in the system can shorten the column lifetime Removal of oxygen can be for all TRACE GC columns are provided below It is important to stay achieved by purging the columns with oxygen free carrier gas for a minimum within the maximum temperature range for the column of 20 minutes at 40 C using an approximate head pressure of 100kPa 2 Monitor the detector signal during conditioning until a stable baseline Altho
20. Thermal Conductivity Detector TCD TRACE GC Ultra and FOCUS GC Item Description 1 Adapter for capillary column Kit Retaining nut for 6mm OD metal column 2 and ferrule 3 Brass front back ferrule for 1 8in OD metal column A Retaining nut for 1 8in OD column and ferrule Nitrogen Phosphorus Detector NPD TRACE GC Ultra Item Description Cat No 34709542 35003062 Cat No 25901110 Quantity Quantity u a f 7 Thermal Conductivity Detector TCD TRACE GC Ultra and FOCUS GC 1 NPD collecting electrode 2 Ceramic insulator 3 Collecting electrode pin 4 Retaining screw 5 TS 2 source standard 5 TS 1 source for ENS mode 6 NPD jet 6 NPD deactivated silcosteel jet 7 Jet removing tool 20501900 Electron Capture Detector ECD TRACE GC Ultra Item Description 1 Upper seal aluminum 2 ECD collecting electrode 3 ECD fixing tool 4 Lower seal silver 5 ECD blocking nut 6 Adapter for stack configuration 7 Heating block for stacked configuration including heater and RTD 8 Coverfor heating block Cat 29032608 24104449 Nitrogen Phosphorus Detector NPD TRACE GC Ultra Electron Capture Detector ECD TRACE GC Ultra ww thermoscientific com chromatography G O CE 3 ED Cp ED O gt O CD 6 CD m D V V z CD 3 CD Ne je z i 26 z 3 145 a
21. a WexMS A sss 2509C TROT 3390 3500 7 JU WaXMS B Css TR B095 36000 370C tt sts S AG OEP TG OCP TD ses AD Osee 82070 3300 35000 1G UPP TZ TG OPP TC s 390 ees 3300C 350C sts S IGALE IZ TGALCI ess 5 o 3300 35000 HI a TRBiodiesel M 3000 32000 i st s JIEM A30 TBiodieselF 2800 3000 _ 7 NE OT NN Ut 93020 3500 RDAS cnn 330 C 0E cosas 93020 39000 eee o TR Pesticide Tn 90 39 C Pesticide ll 300 C 3200 TR Pesticide IV 300 C 320 C ww thermoscientific com chromatography C C a B 5 D gt O CD V CO O 0 V V C D C2 D z C2 3 5 gt D 3 163 Column Conditioning for the TRACE TR WaxMS and TR 1MS Columns This procedure will ensure an ultra low bleed for the columns entire lifetime and is only required once Once performed future installation of the column need only be followed by a 30 minute hold at the maximum temperature limit After installing the column according to the instrument manufacturers instructions follow the procedure below O 0 0 lt CO 0 o O D oteps TR WaxMS TR 1MS 1 Equilibrate the column at 40 with carrier gas flow Equilibrate the column at 40 with carrier gas flow ess L i L20 minutes purging air content s for
22. a column performance that accounts for the effects of unretained elution time where th is the adjusted retention time and is the standard deviation of the peak t mv wes This value also remains constant as retention gaps and guards are used Depending on the method of peak width calculation different efficiencies can be reported This leads to two popular measures 2 Ner 16 Where W is the tangential peak width 13 496 peak height tr Y 5 54 Where W is the width measured at half height 5096 peak height HEEP Her Height Equivalent to an Effective Plate Her L Ner Where L is the column length The smaller the Ne the more efficient the column s performance HETP H Height Equivalent to a Theoretical Plate is a measure of column efficiency where L is the column length and N is the number of theoretical plates L N is based on actual tg rather than adjusted retention times tg Linear Velocity u Mobile phase flow rate expressed in cm s and Is expressed as us Lt Where L is the column length and tm is the breakthrough time of an unretained peak Phase Ratio The ratio of the volume of mobile phase to the stationary phase An important value when changing the column dimensions in a method column ID um 4 x film thickness um Resolution A measure of the separation of two peaks taking into account both the difference in elution time and the peak widt
23. and SPME e Quick mechanical installation of mainframe and accessories e Self alignment procedure and self recognition of all installed accessories e Simultaneous automation of independent sampling methods on two GC GC MS units Rugged SPME automation configurations Sold under license from Supelco C C O B 5 2 gt CD V CO O A V V Ci C zi CD C ge le D 2 Syringe Holders Description Cat No Quantity E Syringe holder for 50mm needles 5 10 100 250pl syringes 28400010 E Syringe holder for 50mm needles 0 6 10 100 500pL syringes 25401015 gt syringe holder for 80mm syringe needles 25401020 1 o 5 10 100 25010 syringes 7 syringe holder for 80mm syringe needles 05 10 100 500yL syringes 20008 EM Y Syringe holder for TriPlus headspace 36803008 1 _ oyringe holder for SPME fiber 25401080 1 Sample Trays Description Cat No Quantity Ambient tray holder configurable in field as primary or secondary 36812755 h m Primary ambient tray positions 1 150 for 1 2 2 5ml vials 240101500 1 _ E Primary ambient tray positions 1 54 for 10 20mL vials 24010160 1 _ E Secondary ambient tray positions 151 300 for 1 2 2 5ml vials 24010155 c 1 amp E Secondary ambient tray positions 55 108 for 10 20mL vials 240101058 4
24. e optimum flow rate to achieve nitrogen s very low HETP leads to long analysis times see figure H He HETP mm Helium has a slightly lower efficiency than nitrogen but the optimum flow rate is higher Also small changes in flow rate of helium around the optimum will not affect efficiency as greatly as with nitrogen For many hydrogen is the carrier gas of choice It shows higher efficiency than helium and at a higher flow rate The variation in HETP 10 20 30 4 5 6 amp 0 70 8 830 with changes in flow rate is also far lower making it more forgiving and reproducible There is however a slight risk of an explosive atmospheric build up in the oven A van Deemter plot of efficiency against linear flow rate for three carrier gases Recommended Flow Rates and Velocities for Capillary Columns Carrier Gas 0 25mm ID 0 32mm ID 0 53mm ID mL min cm min mL min cm min mL min cm min 6 J 1 6 50 2 0 50 1 9 50 0 M MEG GE MEE MNA MN C C O a B 5 D gt O CD ON O x A V V C D C2 D z C2 3 gt D 2 Recommended Detector Gas Flow Rates Detector Air mL min mL min Make Up mL min ED e 350 FID 350 35 30 E M M Seance aan m 2 ME E NE P Unretained Compounds Detector Analyte FID Methane et NPD cman AC TCD MS Methane Butane T i a ww thermoscientific com chromatography 3 161 WEM Selection of
25. epeller 1812004120 Forceps 1876360 0008 1 LENS 0404 1130 0c 1 ISQToa Kit sg 0 k lens2 _ _ 1R920404 1140 1 Does not include exchange tool 11208800071 1 E Lens 3 RF Lens 120101150 1 _ Source Exchange Tool 1R120406 2000 lon Volume 1R120404 4112 Column Measuring Tool 1R120461 0010 1 lon Volume El CI Combo m 18120404 413 Manifold o ring 1R3815 360 1 CI lon Source Cartridge Rit 120101400 1 Vent valve o ring 183814 111 1 Dual Filament 1R120404 1900 1 Oil mist filter 1R76505 0036 1 Electron Multiplier 1876022 14633 1 _ Column Components T10 Torxhead Key 7 1R3812 5T10 _ Ds Description Cat No Quantity 20 Torxhead Key sss 1R3812 5T20 1 Graphite Vespel ferrule NU E 1R3812 5T30 M for 0 25mm columns E octofluoronapthalene 1R120151 TEST 1 ii ee enzophenone Graphite Vespel ferrule 1R 76458 2019 10 for O 32MM COMMS nn Graphite Vespel ferrule 1R76458 2020 10 S Tor S3mmcolumn inane 2 hole graphite Vespel 1R76458 2018 10 E ferrule for lt 0 32mm column 0 E No hole graphite Vespel ferrule 1R76458 2009 10 Sil Tite ferrule for 0 10 0 25mm ID column 1R76458 2000 10 NE ferrule for 0 32mm columns 1R76458 2024 10 Sil Tite ferrule for 0 53mm columns 1R76458 2026 10 gt Nut for SilTite ferrules 1R76458 2001 5 E Nickel coated nut 1R76256 0060 5 g for graphite Vespel ferrule E E 2 2 eo E E 3
26. herefore the contamination It is also dependent on the stationary or ferrule metal can result in poor peak shape due to band broadening phase Generally 2mL of pentane is suitable for non polar contamination at the injection step This can be cured by the removal of a section from With methanol used for more polar samples the front end of the column The amount removed is dependent on the degree of contamination the size of injection and the ID of the column but generally 50cm should be sufficient As the efficiency of the column is proportional to the square root of its length the removal of the front Thermo Scientific Chromatography Columns and Consumables 2012 2013 3 164 Notes e C C 3 5 ia gt CD V O x A V V D D CD 3 5 e D 2 ww thermoscientific com chromatography 3 165
27. hromatography 3 149 50 Innovative Single Quadrupole GC MS Unique Full Source Removal capability delivers maximum uptime and unstoppable productivity e ExtractaBrite solid inert ion source provides robust operations and low detection limits Versatile and flexible for a broad range of applications Re think the way your lab operates with the Thermo Scientific 150 single quadrupole GC MS system The ISQ GC MS incorporates an innovative source design that keeps the 150 cleaner longer With heat throughout the ion optics the 150 can analyze more samples per day with maximum uptime Easy to learn easy to use and easy to maintain the 150 was developed to meet the needs of the modern analytical laboratory O 0 0 lt CO 0 O O lon Source Components Mechanical rotary vane Pump Description Cat No Quantity Description Cat No Quantity E El lon source Cartridge Kit 120404 4100 1 _Oil rotary vane pump A0301 159101 1 lon 1R120404 1105 E Ellon Volume 18120401111 1 Accessories Em eere ean a Description Cat No Quantity NU dila ed Noc EE Aluminum oxide 32000 60340 1 lon Volume Locking Ring 1R120404 1118 1 sette visione ient ituN EM EN EN O nnne Calibration Compound Repeller Spring pkg of5 1R76485 1000K 5 berfuorotributylamine 2 R
28. hs t t R o a 0 5 W W Where t and t are the two retention times and W and W are baseline peak widths Selectivity The relative retention of two adjacent peaks Selectivity can be calculated using capacity factor o Ke Trennzahl Number A value to describe a separation The Trennzahl number is calculated from the resolution between two consecutive homologous hydrocarbons The Trennzahl number represents the number of peaks that can be included between the two hydrocarbon peaks tk t T R2 R1 wi T m Where tr equals analyte retention time and WA equals peak width at half height van Deemter Equation This is a relationship that considers the effect of linear velocity on the HETP or H where A accounts for eddy diffusion B describes the molecular diffusion of the vapor in the direction of the column axis C refers to the resistance to transfer from the stationary to mobile phase and u is the linear velocity of the mobile phase H A Bucy u Carrier Gas Choice The choice of carrier gas is a compromise between a number N of considerations among them efficiency and speed as well as availability safety and cost The three most common carrier gases used are nitrogen helium and hydrogen Nitrogen shows the lowest HETP making it the most efficient of the gases High quality nitrogen is readily available and inexpensive compared to other options However th
29. or and cooling flap Monitor the column temperature GC column is in poor condition Condition the column Change the column Retention Times are Inconsistent Air is leaking into the system at the injector seal Trace and repair the leak or the carrier gas manifold If you need more assistance or information on specific detection systems please contact us and request our Troubleshooting Guide ww thermoscientific com chromatography 3 159 O 0 0 lt CO 0 o O Thermo Scientific Chromatography Columns and Consumables 2012 2013 3 160 GC Equations Adjusted Retention Time t An analyte s retention time tg minus the elution time of an unretained peak tm th th tn Adjusted retention time is also equivalent to the time the analyte spends in the stationary phase Capacity Factor k Expression that measures the degree of retention of an analyte relative to an unretained peak where tp is the retention time for the sample peak and tn is the retention time for an unretained peak A measurement of capacity will help determine whether retention shifts are due to the column capacity factor is changing with retention time changes or the system capacity factor remains constant with retention time changes E tr tm tm Thus the higher the capacity factor the longer the retention time k Effective Theoretical Plates A measure of
30. rrier linear flow rate Increases interaction with the stationary phase Increases retention and resolution but also peak broadening Increase film thickness More interaction with the sample Change the phase More polar columns retain polar compounds better and vice versa Increase column length Maintain linear velocity Improve resolution Reduce temperature ramp rate Increases the distribution co efficient and increases analyte dwell time in the stationary phase Reduce carrier linear flow rate Increases interaction with the stationary phase Increases retention and resolution but also peak broadening Change column dimensions Longer column smaller ID Increase film thickness Can provide discrimination based on time spent in stationary phase Change the phase More polar columns retain polar compounds and vice versa providing analyte discrimination Increase speed Increase temperature ramp rate Reduces the distribution constant analytes spend less time in the stationary phase Increase carrier linear flow rate Decreases interaction with the stationary phase Shorter column Shorter analysis time Decrease film thickness Analytes spend less time in the stationary phase Consider UltraFast GC Can reduce analysis time by a factor of 20 GC Troubleshooting Before you start any troubleshooting it is essential to observe safe laboratory practices Know the chemical and physical properties of
31. ss in more than 15 years It delivers unprecedented flexibility and productivity for large sample series and can optionally be equipped with two TRACE GC Ultra units simultaneously installed at the same ion source The DFS is designed for unattended operation with highest sample capacity and ultimate sampling flexibility MAT95 Double Focusing Sector Field Mass Spectrometer Proven performance for target compound confirmation in environmental food safety and forensic sports doping analysis Versatile for structure elucidation using multiple inlet systems The Thermo Scientific MAT95 XP has been the high end workhorse in the analysis of polychlorinated dioxins and furans for many years It combines unprecedented sensitivity with the ruggedness of a benchtop instrument It is highly versatile and can be equipped with various ionization methods such as El Cl FAB ESI and C C a B 5 D gt O CD ON O A V V Ci C zi CD C gt le D a S 2 High Resolution MS General Spares DFS MAT95 900 High Resolution MS General Spares DFS MAT95 900 Description Cat No Quantity Description Cat No Quantity Filament El Operation Tungsten DIP aluminum crucibles for liquid for DFS MAT95 900 3 ape e Filament Cl Operation Rhenium DIP aluminum crucibles for liquid for DFS MATS5 900 s ee E samples ss nara c lon volume for exchange El operation
32. ty E Magnet support thumbscrew 76483 01259 Endcapelectrode 19650 0520 _ Compression spring n 76485 0032 7 s SPACE ae 119650 0540 Setscrews 76905 0405 1 _ Ring electrode sss 19650 0525 Magnet supportyoke 5 1196500710 Exit enS 119650 0530 _ P Magnet oyj 1R70001 38195 1 Exitlensspacer yj 19650 0515 1 i lon volume holder standard 10001 20532 n 1 NNNM Helium inlet 119650 0532 1 lon volume EI 19650 0220 _ E lon volume Cho 119090 0230 1 InletValve Upgrade Option E D e Description Cat No Quantity lon volume closed exit CEI 119650 0221 T Bat valve seal replacement kit 76461 2002 1 a lon volume holder CElonly 70001 20532 T 1 inlet valve seal replacementkit 49265 0008 1 E Lensheaterring 11965004227 _ ensassembly 8680 0180 Mechanical Rotary vane Pump Lens 1 1196504014 1 Description ss Cat Quantity _ eee 7 Lens3 _ 19650 0418 _ lens spacer 1198650026 Accessories Lens retainer elip 19650 0428 _ Description Cat No Quantity E Flament 20320 0030 Allen wrench kit metric 3812 0100 1 Filament spacer insulator 19650 02355 Alumnumoxde 32000 60340 1 ML LLL LL nnn Calibration compound gk te 1 on source PCB 98000 60087 perfluorotributylamine E lon source 19650 0205 1 Backferule l in brass 0101 02500
33. ugh all Thermo Scientific columns have been pre conditioned is reached Due to the factory pre conditioning of the column this we recommend that they are conditioned after installation by following should be achieved in approximately one hour This duration may be these steps longer in the case of thick films and polar phases Maximum Operating Temperatures for TraceGOLD and TRACE GC Columns Column Maximum Operating Temperature Column Maximum Operating Temperature BP 330 le ae a LU _IGXLBMS OC 36076 55a TAS 340 C 350 C for films lt 1 5um TG 5MS D eee TG SOP 330 C 350 C TR 5MS 360 C 370 C for films lt 1 5um TG 5MSAMINE 300eC 36 0 350 C 360 C for films gt 330 3506 ASAD L 0070 CTG5HT 380 C 400 C s lG35MS 3000 32000 T RA 280 C ls a TG 3BMSAMINE age 1 3600 37 300 32096 25 ey Nu sul ERE st _ eC for films lt 1 um I amp 190MM8 nm 280 C 28096 TRWax 740 C 260 C for films gt 10pm 18 624 VC I _ TR WaxMS 2 80 300 C UTRERAP 240 C 250 GMS OC 260 Ds EN MH Ab Cfor mse lum 77077 164701MS 5s 2606 2806 5a 370 C for 2 65um films TG 225MS 000007 M JIEMS s TRFAME 250 C 260 0 sts s S DLA 0 2occ 260 C IG WexMS cos RSD 340 C 360 0
34. uu lens3 119650043 1 _ Lens spacer oss 19650 0426 1 DSO II Accessories and Spares Lens retainer clip 119650 0428 1 pe Inlet Valve Upgrade Option nn d19800 0535 1 Description Cat No Quantity a Prefilter lens retainer clip 120173 0002 _ Ball valve seal replacement kit 16461 2002 iD n uo EE pubes e Bo DQUUU P T a NM m Lens retainer clip 120320 00408 1 Description Cat No Quantity M iocos EE MM Allen wrench kit metric 3812 0100 1 2 onvolumeindexscrew 120320 0042 Aluminumoxde 32000 603440 E Filament 12032000090 1 Backfemule 1 8in brass A0101 02500 1 cr DO I o Calibration compound sss 4 5 Filament retainer 120320 0050 1 perfluorotributylamine 1202718601 QUT a T TA E du em Front ferrule 1 8in brass 40101 08500 Test mix octafluoronaphthalene 120150 TEST 1 benzophenone sss Transfer ine fere 1 1in to HN Ne graphite vespel Mechanical rotary vane Pump Description Cat No Quantity Oil rotary vane pump A0301 15101 1 ww thermoscientific com chromatography 3 151 cn O 0 0 D O lt CO 0 o O O Thermo Scientific Chromatography Columns and Consumables 20
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