Installation and User Manual for PiezoSleep
Contents
1. 08 14 C Viewing sensor signals for multiple active channels Signal Panel Tab 15 D Monitoring percent sleep for all active channels Sleep Summary Tab 17 E Monitoring decision statistics for all active channels Decision Statistics Tab 18 F Current file and setup information display Setup Info Tab cceeceeeseeeeeeees 19 G Monitoring signals from auxiliary sensors Auxiliary Data Talb ccccccseeeee 20 3 File INfOPMATION cccccccceccccccceccccecccecccceceuceceneaceceguenecueaueaeaneaeeneaceeauecesneaeentanentanes 21 1 Introduction The PiezoSleep software was written and compiled in LabVIEW National Instruments Austin TX to monitor and record high throughput mouse behavior tracking experiments The program creates several files for classifying behavioral states The base name for the files is provided by the user when the experiment starts up and extensions and suffixes are added to distinguish the different files The basic pressure signal from the piezoelectric sensors is sampled at 120 Hz and saved with a bin extension Features associated with sleep and wake behaviors are extracted from the pressure signal and saved every 2 seconds in a file with the FeatVec extension The FeatVec file is read in by an analysis program to characterize the sleep and wake behaviors over the duration of the experimental recording The breath rate is estimated during sleep s2. 2014 Signal Solutions LLC PiezoSleep Figure 16 Decision Statistics tab showing histograms of 4 channels simultaneously The Page box can be used to switch to other active channels if more than 4 channels are active F Current file and setup information display Setup Info tab After data collection has begun current information and settings will be displayed on the Setup Info tab as shown in figure 17 The display includes Start Time Number of active channels Primary and Secondary DAQ selections and their channel information pseudo LED showing Auxiliary DAQ usage File Path and Light and Dark transition times These selections were made by the user at the start of the data acquisition session The start time is stored in the data file header so it is saved with the data to create an absolute time axis for computing statistics related to the light and dark periods as well as confirming the date the experiment began 20 3 PiezoSleep eo iz mmn LS eB Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Amdkary Data ng Sig p ry ry Start Time 11 31 50 AM 2 19 2014 Number of active channels 100 Primary DAQ USB 6225 Recording Channels 1 to 80 Secondary DAQ USB 6218 Recording Channels 81 to 100 Actual Num of DAQs in use 2 Using Auxillary DAQ File Path 3 C Users signal solutions Documents Lab VIEW Data tt bin H Dark to Light Time 7 0 M 0 H M Light to Da
3. so relative amplitudes can be observed The primary purpose of these graphs is to view signals from all cages 16 quickly to see if there are problems broken connections amplifiers not plugged in dead mice etc However by right clicking on the y axes of these graphs you can turn on the auto scale to see more detail for weaker signals To go to a fixed scale set to manual scale and directly click on the y axis numbers maximum and minimum and type over to set the limits There is a 2 second delay between what is happening in the cage and what appears on the screen A Bres a L kaas Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Auxiliary Data J J Channel 1 Animali Channel 2 Animal2 o Channel 4 Animal4 Page Total pages pl 25 Copyright 2014 Signal Solutions LLC pezoslee si B F PiezoSieep oe a Sa Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Auxiliary Data Channel 1 Animali Channel 2 Animal2 0 0100757 0 05 0 005 0 97 0 005 0 010988 Channel 4 Animal4 0 0944681 Channel 3 Animal3 0 018701 0 0134681 0 01 0 0 01 0 0157927 0 0120692 _Page Total pages wy z spl 25 Copyright 2014 Signal Solutions LLC PiezoSleep STOP Fi
4. 6 8 10 Sleeping _ Breath rate of selected channel ZSZ Copyright 2014 Signal Solutions LLC FH PiezoSleep Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Auxiliary Data Channel to analyze 7 1 Filtered signal of the selected channel 15 4 1 at 3 3 E 0 5 3 lt 05 O 5 A oe Ag 45 3 a o Time Welcome Power spectrum of Select number of DAQs to use for this session 50 60 1 me a 70 3 u 80 w E E 0 99 a E lt 100 co 95 110 120 i 1 1 1 0 25 5 75 10 12 5 15 1 Frequency Hz Figure 1 Starting windows for PiezoS leep program From the dialog window select the number of DAQs being used for the session B If you are using a single DAQ the window shown in Fig 2 appears containing a table listing all NI DAQ s currently plugged in and recognized by your PC Select the DAQ you wish to use by clicking on its name inside the table cell and then press OK A small window displaying your DAQ selection as shown in Fig 2B will appear Press OK to continue In case of using two DAQs A primary and secondary DAQ must be chosen This affects how the sequence of channels is assigned in PiezoS leep The program first prompts you to select the primary DAQ as shown in Fig 3A The primary DAQ is the one connected to the first set of cages For example in Fig 3A USB 6225 is selected as t
5. SIGNALS Sensor Systems Signal Analysis Software Installation and User Manual for PiezoSleep Version 1 May 2014 High Throughput Pressure and Motion Recording Software with Real Time Classification and Monitoring of Animal Sleep Wake Behavior Ta PiezoSleep i is J n Am Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Auxiliary Data Channel to analyze J 1 Filtered signal of the selected channel 1 Sleeping Histogram of the selected channel 0 1 50 7 40 Observed s 8 Amplitude jd l 0 i i i 1 i D i l 1 i i Time sec 8 10 8 6 4 2 0 2 4 6 8 10 Awake Sleeping Power spectrum of selected channel Breath rate of selected channel AN Ww in Amplitude dB rate Fz oh he Gn D o ge Copyright 2014 Signal Solutions LLC PiezoSleep Signal Solutions LLC Lexington KY www sigsoln com Copyright 2014 Installation and User Manual for PiezoSleep Ver 1 0 Anuj Agarwal and Kevin D Donohue May 2014 Contents Wee HO CUNO IN erea E E E E EE E EEA tesa tned Sgeasenivosadent test soeeeoess 3 A WS et HU ON eenean eneee E EEEE EEE EEEE EEEE E EEEE EA A EE EEE 4 2 Bala aC Onana MONON oee R EE E EE A EET 5 A Starting a data acquisition Session ccccceeccceeeeceeeceeeeceeeeseeeeseeeesaueeseueeseeeeseaess 5 B Selecting and monitoring a channel signal Single Channel Tab
6. The file with the Feat extension and _ br padded to the base name stores the breath rates for all channels The file with the FeatVec extension stores the feature vector used to calculate the sleep Statistics The file with the bin extension and _Aux padded to the base name stores the auxiliary data for all channels The FeatVec file is used by the analysis programs in the MouseRecDataTools toolbox developed by Signal Solutions LLC These files are updated to the disk at least once every 2 seconds during the data acquisition session Therefore if the system crashes due to a power failure data up to the point when the computer system failed are saved The program samples the piezoelectric signals at 120 Hz and 16 bits 2 bytes per sample per channel A 16 channel recording will write 332 MB of data per day If disk storage is limited it may be necessary to periodically stop data collection remove existing data files and restart the recording with a new file This however complicates 22 file and data management It is best to have a computer with sufficient hard disk space and removable media to transport the large files to other systems for archiving and analysis The size of the raw piezo data file can be estimated in terms of the number of days and active channels by the following formula _ 2 24 60 120 d C B 10 x 20 74 dC where dis the number of days C is the number of active channels and B is the file size in mega
7. a file name as shown in Fig 7 Animal motion pressure data will be saved to this file for later processing If a file of the same name already exists in the location the program will prompt you with an option to overwrite the existing file as shown in Fig 8 It is recommended that this file be given a descriptive name that identifies the nature of the experiment or mice and the date the experiment began It is also recommended that NO extension be given to the file name since PiezoSleep adds its own extensions that are used in later programs the bin extension is given to the files with the raw sensor data The software that analyzes data after the experiment uses the base name to look for processed files and opens them automatically If they have different names and extensions the automatic loading will not work and you will be prompted to search for them with a file directory navigator ce Enter file name to store data from this session p ee do Libraries Documents LabVIEW Data Organize New folder A JY Favorites Documents library T Desktop LabVIEW Data pr Downloads Recent Places SkyDrive Arrange by Folder v Name Date modified d LVAutoSave 1 31 2014 12 26 PM di Dependencies 1 30 2014 9 39 AM eee Jy LVRSS 1 30 2014 9 34 AM A Document J MouseRec_BR tests 1 28 2014 10 03 AM T PEA I Glyphs 12 20 2013 4 59 PM d Icon Templates 12 13 2013 3 18 PM Pictures dO Remote Developmen
8. aining phase of the classifier i e animal is ill dead or has a very unusual respiratory pattern during sleep The bimodal pattern in the histogram below is exploited by adaptive thresholding used in later analysis programs which seeks a minimum point between the clusters modes of decision statistics Real time analysis is limited in that it uses the same threshold over all channels and can vary by as much as 8 from the later analysis using an adaptive threshold The primary purpose of real time monitoring in the acquisition program is to alert those monitoring the experiment If problems arise they can be verified by direct observation of the animal or corrected during the experiment More accurate sleep and wake parameters are generated with the sleepstats program in the MouseRecDataTools toolbox 19 0 Pez Shee Si la mu Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Raw Data Channel 37 144740 Channel 38 141795 5000 4000 3000 3 2000 1000 0 Dy 1 l l l l I I l i i 10 8 6 4 2 0 2 4 6 8 1 amplitude pa 40 141992 I 1 I I I I I j I I 10 8 6 4 2 0 2 4 Si 8 3 amplitude Crane 39 144763 5 ia E 2000 2000 3 1000 1000 0 i I i I I i l l i 0 l l l l I l 1 10 8 6 4 2 0 2 4 6 8 1 10 8 6 4 2 0 2 4 6 E cy amplitude amplitude Page Total Pages 10 18 i z ae Copyright
9. am of the selected channel alm 01 50 So 0 05 lu 0 Amplitude Observed hth o he Oo l Time sec 8 Power spectrum of selected channel AN l Breath rate of selected channel A as n I ee in ho ul I L I Amplitude dB Breath rate Hz 5 7 5 10 12 5 ___ Frequency Hz _ Copyright 2014 Signal Solutions LLC PiezoSleep Figure 13 Single Channel tab showing the recorded signal top left sleep decision statistic top right power spectrum bottom left and the breath rate frequency bottom right for an individual channel C Monitoring sensor signals for all active channels Signal Panel tab To simultaneously view sensor signals for the active channels click the Signal Panel tab The Signal Panel will appear as shown in Fig 14 and display 4 active channel signals over the last 8 seconds If there are more than 4 active channels the Page text box can be incremented to page through the rest of the channels The animal IDs with their corresponding channel number are displayed over each plot The thin green bar virtual LED above each plot on the Signal Panel is the sleep indicator A lit indicator implies detected sleep as seen for channel 1 in the figure The y axes of the graphs on the Signal Panel are fixed from 1 to 1 These graphs by default do not auto scale the signal like the filtered signal plot on the Single Channel panel
10. bytes MB The Feat and FeatVec file are considerably smaller in size as compared to the piezo bin file The Feat file consumes space of about 0 34 MB day and the FeatVec file consumes roughly 1 4 MB day for each channel as compared to the 20 74 MB day for the bin file The auxiliary data is sampled at 120 Hz and 36 bits 4 bytes per sample per channel The size of the auxiliary data file B estimated in terms of the number of days d and active channels C is given by B 41 48 dC A 4 channel recording will write 166 MB of auxiliary data per day
11. e a i Figure 15 Sleep percentage summary 18 E Monitoring decision statistics for all active channels Decision Statistics tab To simultaneously view the sleep wake decision statistics for the active channels click the Decision Statistics tab which shows histograms for 4 active channels at a time as illustrated in Fig 16 The animal IDs with their corresponding channel number are displayed over each plot If there are more than 4 active channels the Page text box can be incremented to view results from the rest of the channels This provides a quick way to examine the data collection and sleep wake behavior over the experiment Unusual behaviors or data collection problems can be identified while the experiment is running which can be addressed by direct observation of the animal For example after running the program for 24 hours with a normal animal a clear bimodal distribution should appear in the histograms as a result of mapping sleep signal dynamics into positive values and wake signal dynamics into negative values If the histogram is not bimodal it could be the result of noise or weak signals for which there are a number of Causes e an electrically or mechanically noisy environment e poor animal contact with the piezoelectric sensor on the floor of the cage sometimes cause by too much bedding e faulty amplifier or sensor e unusual animal behavior not observed in the tr
12. gure 14 Signal Panel tab displaying piezoelectric sensor signals for 4 active channels The Page box is used to switch to other active channels when more than 4 channels are active A Y axis fixed default and B y axis switched to auto scale shown with a simulated sleep signal plugged in channel 1 17 D Monitoring percent sleep for all active channels Sleep Summary tab The percentage sleep for each channel can be observed at any point during the recording in the Sleep Summary panel shown in Fig 15 The percentage is based on the sleep threshold and the histogram formed from the accumulating data The example shown below has one simulated sleep signal plugged into channel 1 with detected sleep percentage greater than 99 and the rest are noise only channels with sleep percentages all less than 8 Outliers can be identified here early in the experiment and can be followed up with direct observation of the animal or checking the function of the sensor e g ensure cable is plugged in 49 PiezoSleep x all p mmm Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Auxiliary Data Sleep Summary Channel Percent Sleep 4 Number Update in s 6 r 9 51 5 35 7 42 6 55 5 88 5 88 5 88 5 61 0 15 0 15 0 15 0 15 0 15 0 15 0 15 0 15 x PiezoSleep On ow amp WN oO pa ee N ma Ww D ma uw R
13. have made the following selections Primary DAQ device Secondary DAQ device Number of active channels USB 6225 USB 6218 100 File Path gq C Users signal solutions Documents LabVIEW Data tt bin H Dark to Light time Light to Dark time Animal ID s Select one option from the menu below Channel number ID i _and hit OK Re select DAQ device s ree Re select Number of Active Channels Animab Re select Animal ID s and Light Dark times Animal Re select Filename and Location Animal Animal9 No re selection needed Animall0 Animalil Animall2 Animall3 Animall4 xj wj Figure 9 Prompt displaying user selections and the re selection options H Next the program prompts for the use an auxiliary DAQ as shown in Fig 10 This DAQ can be used to record auxiliary data such as room temperature light intensity humidity etc If you select NO the program starts the data acquisition Steps and J given below contain instructions for setting up the auxiliary DAQ If you are not using an auxiliary DAQ jump to step K for further instructions r k E E o d Figure 10 Prompt to select the use of an auxiliary DAQ 12 The auxiliary DAQ is set up in a way similar to the primary DAQ The program prompts you to first select the DAQ device followed by the number of active channels and then the channel IDs Figure 11 shows these prompts sequentia
14. he primary DAQ i e cages 1 to 80 maximum channels supported by USB 6225 should be connected to it After selecting the primary DAQ the program will prompt you to select the secondary DAQ as shown in Fig 3B The cages numbered 81 in case of USB 6225 and onwards should be connected to this DAQ If you are using two DAQs of the same kind the primary and secondary DAQs can be identified by their serial numbers listed next to the device name in the table 49 Select DAQ om Select the DAQ device you want to use from the table and press OK Device USB 6218 _USB 6000 Figure 2 Prompt to select a single DAQ Consists of a table listing all DAQs currently plugged in your PC Click on a name inside the table and press OK Select Primary DAO E 53 Select Secondary DAQ iiss ene Select the Primary DAQ device from the table and Select the Secondary DAQ device from the table and press OK press OK Device Serial No A Device Serial No USB 6218 25728695 usBe 6218 25728695 USB 6225 24297480 _USB 6000 25963964 USB 6000 25963964 A B Figure 3 Prompt to select the A primary and B secondary DAQ If you are using two DAQs of the same kind the primary and secondary DAQs can be identified by their serial numbers listed next to the device name in the table C Next the program prompts for the number of active channels with the dialog bo
15. ional Instruments folder under the Program files x86 folder in C drive When removing this software the PiezoSleep program can be identified in the Program option under the Control Panel from the PC menus and removed lf you have a USB DAQ plug it in after the software installation is complete If you have a PCI card turn off the computer now and install it Turn the computer back on for the next step If you are using two USB DAQs to extend the number of channels collecting data they need to be synchronized using external digital lines called PFI lines present on the DAQ Physically connect the digital lines PFI O and PFI 1 of one DAQ to the PFI O and PFI 1 of the other DAQ respectively When launching the PiezoSleep software the order of channel assignments to the DAQs can be selected 2 Data acquisition and monitoring A Starting a data acquisition session A Launch PiezoS leep either from the desktop shortcut or from the start menu or from the location it was saved installed on the hard drive The windows shown in PiezoSleep software can simultaneously acquire piezo sensor data from two NI DAQs This is useful if the number of animals to be monitored is larger than the maximum channels supported by a single DAQ Select the number of DAQs you will be using to Fig 1 appear after collect data in the dialog window launching the program Sleeping Histogram of the selected channel am i 1 ic o e a 2 0 2 4
16. lly B 4 Enter Number of Active Auxiliary Channels oc 5c Select the Auxiliary DAQ device from the table and press OK A 4H Select Auxiliary DAQ Enter the number of auxiliary channels to record integer value from 1 to 8 Device Serial No j USB 6218 25728695 Number of Active Channels T Enter Auxiliary IDs Auxiliary IDs Channel ID Number Ambient Light Temperature Pressure Humidity Figure 11 Steps to set up an auxiliary DAQ A Select the DAQ device B Enter the number of active channels C Enter the auxiliary channel IDs 13 J Next the program displays your auxiliary selections and a re selection menu as shown in Fig 12 If needed you can re select the auxiliary DAQ device number of active channels or channel IDs If no re selection is needed then select the last option and click OK to start data acquisition i 4 Aux user selections and re selection menu ee You have made the following selections Auxiliary DAQ device Number of active channels USB 6000 4 Auxillary ID s Channel number ID 1 Ambient Light Select one option from the menu below and hit OK Temperature 2 3 Pressure 4 Humidity Re select Auxiliary DAQ Device A Re select Number of Auxiliary Channels de Re select Auxiliary ID names o No re selection needed Figure 12 Prompt di
17. nant peak at around 3 Hz The amplitude on the y axis is expressed in the logarithmic unit decibels dB The sleep decision statistic comprises of a histogram plot showing the sleep wake distribution for the selected channel The sleep wake state with about a 4 second lag from real time is shown via a green LED located just above the panel which lights up to indicate sleep Detection is based on comparing the decision statistics to a sleep threshold which by default is Zero and is depicted on the histogram by a blue vertical line Larger positive decision statistics indicate a greater likelihood of sleep while negative decision statistics indicate a greater likelinood of wake In Fig 13 the green LED is lit indicating a current sleep state and the histogram shows a larger concentration on the positive side indicating that the animal has been asleep for some time The breath rate plot displays the most recent 32 minutes of data updating itself once every 2 seconds For the first 32 minutes after starting the program the breath rate plot will accumulate data and from there on plot the most recent 32 minutes of data The breath rate plot in Fig 13 shows that the breathing frequency of the animal was around 3 Hz 15 3 PiezoSleep L a m ecli o a Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Auxiliary Data Channel to analyze 1 Filtered signal of the selected channel Sleeping Histogr
18. r of active channels selected If you select No then you have the option to enter IDs manually for each active channel or use the default channel labels 3 Enter Number of Active Channels Enter the number of active channels to record integer value from 1 to 80 Number of Active Channels 7 31 Figure 5 Import request for spreadsheet E After the spreadsheet prompt is complete or dismissed another dialog box will pop up as shown in Fig 6 The dark to light and light to dark transition times can be entered in the designated area where the times are in military format 0 to 24 These time values are stored in the data file headers and used in a later analysis program to compare sleep behavior during the light and dark periods If light and dark onset times are not critical then these can be left in their default settings and disregarded or changed in the analysis program This entry will not affect the data acquisition In this same window the animal IDs can also be changed to meaningful labels if desired These ID labels are also stored in the header so subsequent analysis programs can identify each channel with these labels When finished entering this information click OK to proceed gj Channel Setup E H Dark to Light Time 07 H Figure 6 Prompt to enter labels for recorded channels and the light and dark transition times used during the experiment Next the program prompts for
19. rk Time 19 0 0 Copyright 2014 Signal Solutions LLC PiezoSleep Figure 17 Setup Info tab G Monitoring signals from auxiliary sensors Auxiliary Data tab The Auxiliary Data tab simultaneously displays 4 signals from auxiliary sensors as shown in Fig 18 If there are more than 4 active channels the Page text box can be incremented to page through the rest of the auxiliary channels The auxiliary IDs with their corresponding channel number are displayed over each plot Auxiliary data tab provides a quick way to notice ambient disruptions such as in temperature or humidity control as these can have a pronounced effect on the sleep wake characteristics 21 i PiezoSleep Single Channel Signal Panel Sleep Summary Decision Statistics Setup Info Auxiliary Data Ambient Light Temperature 4 92994 4 5 4 35 3 25 1 86731 Pressure idi vr p Copyright 2014 Signal Solutions LLC PiezoSleep Figure 18 Auxiliary Data tab example showing 4 pseudo auxiliary channels Note that the output on y axis seen here is in terms of voltage and is not calibrated to its respective units 3 File Information PiezoSleep saves three piezo data files and one auxiliary data file if selected for use The file with a base name provided at the beginning of the data acquisition session and the bin extension stores the raw data from the piezo sensors
20. s XP operating systems 32 or 64 bit and an available PCI slot for NI PCI DAQs or a USB port for the NI USB DAQs The installation of the PiezoSleep software automatically installs drivers for the NI DAQ if they are not already installed Updated drivers and the runtime engine required to run PiezoSleep are freely available from National Instruments at http www ni com drivers A li Installation To install the real time sleep wake monitoring software and supporting National Instruments NI software download and unzip the installation package via instructions given with your purchase Transfer the file to the hard drive of the computer where data will be collected and unzip the file Once unzipped the directory PiezoSleep_1 should have been created Inside this directory is one labeled volume and inside this is an application file named setup Double click execute this file to start the installation process Follow the instructions in the prompts to complete the installation Once the installation process is complete a shortcut named PiezoSleep will be created on the desktop Double clicking on the shortcut will launch the software program The PiezoSleep program can be also accessed from the start menu under the PiezoSleep folder or from the hard drive location it was saved to during the installation The software requires the NI LabVIEW Run Time Engine 2013 which is installed along with PiezoSleep and is present in the Nat
21. splaying user selections and the re selection options for the auxiliary DAQ K The program now starts recording and displaying data Under each tab the waveforms statistics will begin to appear in a few seconds the details of which are explained in the following paragraphs L When data collection is finished click the STOP button located in the bottom of the window A new test can then be started by clicking the arrow button located on the top left corner or the program can be terminated by clicking the X in the upper right corner to close the window 14 B Selecting and monitoring a channel signal Single Channel tab The Single Channel tab displays the most recent 8 seconds of the recorded signal along with additional graphical descriptions As shown in Fig 13 the tab consists of four panels displaying the pressure signal top left sleep decision statistic top right power spectrum bottom left and the breath rate frequency bottom right for a given channel To view a particular channel the channel number can be typed into the Channel to analyze text box or incremented up and down with the arrows to the left of the text box To facilitate real time observation the PiezoSleep program computes and updates the power spectrum breath rate and the sleep decision statistics every 2 seconds The power spectrum shows the signal s power distribution over a range of frequencies The power spectrum example in Fig 13 shows one domi
22. t 10 5 2013 4 09 PM Subversion BE Videos o AppBuilderCache 10 5 2013 4 04 PM D vicnvrt 10 5 2013 12 46 PM d VIObjCache 10 5 2013 12 45 PM d LVInternalReports 7 2013 12 14 PM d Probes 11 12 2012 7 37 PM tt bin 1 31 2014 12 35 PM a Homegroup J Computer amp 0s C 7 4 fe MEL File name v binary files bin OK lr 7 Figure 7 Prompt to enter a filename where data is to be stored during the experiment C Users signal solutions Documents LabVIEW Data test bin This file already exists Do you want to replace it Cancel Figure 8 Overwrite existing file G After selecting the filename and location the program displays your selections as shown in Fig 9 The display includes the DAQ device name primary and or secondary number of active channels file path light and dark onset times and animal ID names It also contains a re selection menu which gives you the option to re select the DAQ device number of active channels animal ID names or filename and location If no re selection is needed then select the last option and click OK to continue Please note that if you re select the DAQ device you will also be prompted to re select the number of active channels and the animal ID names Similarly if you re select the number of active channels you will also be prompted to re select the animal ID names 11 3 User selections and re selection menu You
23. tates every 2 seconds and stored in a file with a suffix and extension _br Feat This file is also open by the analysis program for characterizing the sleeping breath rates In addition to data related to the pressure signal the program can also measure and save auxiliary data from a separate data acquisition module and saves to a file with the suffix and extension Aux bin This feature requires an additional analog to digital converter to input parameters such as ambient temperature light intensity humidity etc While the recording is in progress the graphical user interface of PiezoSleep provides a variety of graphs statistical summaries and current sleep wake states to monitor the data collection for real time monitoring The PiezoSleep software supports the use of either PCI or USB NI data acquisition devices NIDAQ for simultaneous recording and monitoring of multiple channels Examples of DAQ devices are PiezoS eep is not limited to these only The NI USB6009 DAQ records 1 to 8 input channels The NI PCI6220 DAQ records 1 to 16 input channels The NI PCI6224 DAQ records 1 to 32 input channels The NI USB6218 DAQ records 1 to 32 input channels The NI USB6229 DAQ records 1 to 32 input channels The NI PCI6225 DAQ records 1 to 40 input channels The NI USB6225 DAQ records 1 to 80 input channels The software described in this document automatically detects all DAQ s configured on your PC Software will run on a PC with Windows 7 or Window
24. x shown in Fig 4 This is done so that only the necessary channels will be recorded The maximum number of channels is based on the DAQ capabilities and is listed in the prompt Type in the maximum number of channels if all cages are being used in the experiment If fewer channels than the maximum will be used then the number of active channels can be entered to save storage space and result in the later analysis programs running more efficiently For example if only four animals are to be monitored cages 1 to 4 then 4 should be entered as the number of active channels In this case the mice should be placed sequentially in the cages i e the first one in cage 1 the next in cage 2 Ifa cage is skipped in the sequence then enter the number of channels corresponding to the highest cage number containing an animal For example if mice are in cages 1 2 5 and 6 then 6 should be entered for the number of active channels Channels 3 and 4 in this case will be recorded but they will contain only noise and should be marked as empty on the channel labels so they can be ignored in future analyses D After you enter the number of active channels the dialog box shown in Fig 5 appears asking if you wish to import animal ID names from an Excel spreadsheet If you have a spreadsheet file xls or xlsx with the animal IDs the format should be a series of names text format under column A and the number of rows should be equal to the numbe
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