HI 96801 Refractometer for Sucrose Measurements
Contents
1. DISTILLED WATER de ee 3 Press the ZERO key If no error messages appear your unit is calibrated For a description of ERROR MESSAGES see page 7 Note The 0 0 screen will remain until a sample is measured or the power is turned off ATC x n m ATC LI LI eau esd 4 Gently absorb the ZERO water standard with a soft tissue Use care not to scratch the prism surface Wipe off the surface completely The instrument is ready for sample measurement Note If instrument is turned off the calibration will not be lost e MEASUREMENT Verify the instrument has been calibrated before taking measurements 1 Wipe off prism surface located at the bottom of the sample well Ow E 2 Using plastic pipettes drip sample onto the prism surface Fill the well completely Note If the temperature of the sample differs significantly from the temperature of the instrument wait approximately 1 minute to allow thermal equilibration 7 FR FP 3 Press the READ key Measurement is displayed in units of BRIX Note The ATC tag blinks and automatic temperature compensation is disabled if the temperature exceeds the 10 40 C 50 1049F range 4 Remove sample from the sample well by absorbing on a soft tissue 5 Using plastic pipettes rinse prism and sample well with distilled or deionized water Wipe dry The instrument is ready for the next sample a Q DISTILLED WATER MAKING A2. Fax 02 2758 683 Germany Tel 07851 9129 0 Fax 07851 9129 99 Greece Tel 210 823 5192 Fax 210 884 0210 Indonesia Tel 210 4584 2941 Fax 210 4584 2942 Japan Tel 03 3258 9565 Fax 03 3258 9567 Korea Tel 02 2278 5147 Fax 02 2264 1729 Malaysia Tel 603 5638 9940 Fax 603 5638 9829 Singapore Tel 6296 7118 Fax 6291 6906 South Africa Tel 011 615 6076 Fax 011 615 8582 Taiwan Tel 886 2 2739 3014 Fax 886 2 2739 2983 Thailand Tel 66 2619 0708 Fax 66 2619 0061 United Kingdom Tel 01525 850 855 Fax 01525 853 668 USA Tel 401 765 7500 Fax 401 765 7575 80 2 I L0896NVIA For e mail contacts and a complete list of Sales and Technical offices please see www hannainst com
3. STANDARD BRIX SOLUTION To make a Brix Solution follow the procedure below e Place container such as a glass vial or dropper bottle that has a cover on an analytical balance e Tare the balance e To make an X BRIX solution weigh out X grams of high purity Sucrose CAS 57 50 1 directly into the container e Add distilled or deionized water to the container so the total weight of the solution is 100g e Note Solutions above 60 Brix need to be vigorously stirred or shaken and heated in a water bath Remove solution from bath when sucrose has dissolved The total quantity can be scaled proportionally for smaller containers but accuracy may be sacrificed Example with 2596 Brix Vo Brix g Sucrose g Water g Total 25 25 000 75 000 100 000 TO CHANGE TEMPERATURE UNITS To change the temperature measurement unit from Celsius to Fahrenheit or vice versa follow this procedure 1 Press and hold the ON OFF key continuously for aproximately 15 seconds The LCD will display the all segment screen followed by a screen with the model number on the primary display and the version number on the secondary display Continue pressing the ON OFF key 15 seconds HBAR Bn i BBBB i17 2 While continuing to hold the ON OFF key press the ZERO key The temperature unit will change from C to F or vice versa ae Cor F BATTERY REPLACEMENT To replace the instrument s battery follow these steps Turn the inst
4. Instruction Manual HI 96801 Refractometer for Sucrose Measurements FINN instruments w hannainst com WwW Dear Customer Thank you for choosing a Hanna product This manual will provide you with the necessary information for the correct use of the instrument Please read it carefully before using the meter If you need additional technical information do not hesitate to e mail us at tech cOhannainst com This instrument is in compliance with C directives TABLE OF CONTENTS PRELIMINARY EXAMINATION scettr nito 2 GENERAL DESCRIPTION satio ri teil e aet etae ta 3 EE ni vicc sores ere 3 PRINCIPLE OF OPERATION Luanda dinne 4 FUNCTIONAL DESCRIPTION cadentia reinen eine uertit cene 5 DISPLAY ELEMENTS ceci orta qnia Rn esi Quat tiu Rice 6 WARNING cuia din A a RE Rc EN 6 ERROR ME ASAE OE EE ER PE 7 CALIBRATION PROCEDURE ERE n uhi 8 MEASUREMENT PROCEDURE aaret raude 9 MAKING A STANDARD BRIX SOLUTION 10 CHANGING TEMPERATURE UNITS csscscossssssiessstsscosssaissesnisestensnsnnsacensenisseitsssniisiiosorsiniee 10 BATTERY REPLACEMENT eintritt deett 11 WARRANTY c 1 PRELIMINARY EXAMINATION Remove the instrument from the packing materials and examine carefully to ensure no damage has occurred during shipping If any damage has occurred notify your Dealer or closest Hanna Customer Service Center Each HI 96801 instrument is supplied with e 9 V battery Instruction manu
5. al Note Save all packing material until you are sure that the instrument functions correctly A defective instrument must be returned in its original packing MODEL IDENTIFICATION HI 96801 All rights are reserved Reproduction in whole or in part is prohibited without the written consent of the copyright owner Hanna Instruments Inc Woonsocket Rhode Island 02895 USA GENERAL DESCRIPTION The HI 96801 Digital Sucrose Refractometer is a rugged portable water resistant device that benefits from Hanna s years of experience as a manufacturer of analytical instruments The HI 96801 is an optical instrument that employs the measurement of refractive index to determine the 96 Brix of sugar in aqueous solutions The method is both simple and quick Samples are measured after a simple user calibration with deionized or distilled water Within seconds the instrument measures the refractive index of the sample and converts it to Brix concentration units The HI 96801 digital refractometer eliminates the uncertainity associated with mechanical refractometers and is easily portable for measurements in the field The measurement technique and temperature compensation employ methodology recommended in the ICUMSA Methods Book Internationally recognized body for Sugar Analysis Temperature in C or F is displayed simultaneously with the measurement on the large dual level display along with icons for Low Power and other helpful message codes Key
6. bration HI Top display Sample exceeds maximum n H rnm H C measurement range va ULL Wrong calibration used to zero M i ee bes oe instrument Use deionized or 3 LM distilled water Press Zero e HI Top disola 1 Wrong calibration used to zero 1 SETUP Fadory Calibration Tag Cal DN Ei instrument Use deionized or 2 Measurement in Progress Tag ae Weter Press Zero 3 Battery blinks when low battery condition detected t LO Top displa Temperature exceeds ATC low limit 4 Calibration Tag Cal segment 0 10 C during calibration 5 Automatic Temperature Compensation F blinks when temperature exceeds 10 40 C 50 104 F range t HI Top display Temperature exceeds ATC high limit Cal segment O 3 40 C during calibration 6 Primary Display displays measurement and error messages i 7 Temperature Units l aoe 8 Secondary Display displays temperature measurements when blinking jj Prism surface ifisiticiently covered temperature has exceeded operation range 0 80 C 32 176 F f Too much external light for measurement Cover sample well MEASUREMENT GUIDELINES D with hand e Handle instrument carefully Do not drop LED light is not detected e Do not immerse instrument under water D ene e Do not spray water to any part of instrument except the sample well located over the prism e The instrument is intended to measure Sucrose solutions Do not expose
7. e reflected off the interface The critical angle can be used to easily calculate the refractive index according to the equation sin 0 E n n Where n is the refractive index of the lower density medium n is the refractive index of the higher density medium Refraction Critical Angle Total Internal Reflection 6 Bcritical 6 Bcritical 6 gt B critical 0 angle of incidence N4 Ny refractive index 0 angle of refraction In the HI 96801 light from an LED passes through a prism in contact with the sample An image sensor determines the critical angle at which the light is no longer refracted through the sample The HI 96801 automatically applies temperature compensation to the measurement and converts the refractive index of the sample to sucrose concentration in units of percent by weight Brix Sample Well Linear Image Sensor FUNCTIONAL DESCRIPTION TOP VIEW BOTTOM 1 Liquid Crystal Display LCD 2 Read Key User Measurement 3 Zero Key User Calibration 4 On Off 5 Primary Display 6 Secondary Display 7 Stainless Steel Sample Well and Prism 8 Battery Cover 9 Battery Compartment ST TE ERROR MESSAGES Error Code Description General failure Cyde power to N instrument If instrument still has ATC B error contact Hanna r1 Ti ras r1 Sample is reading lower than the H Li H H LO Top display 0 96 BRIX standard used for meter LI LI Li i T cali
8. features include e Dual level LCD Automatic Temperature Compensation ATC Easy setup and storage Battery operation with Low Power indicator BEPS e Automatically turns off after 3 minutes of non use SPECIFICATIONS Range 0 to 85 Brix 0 to 80 C 32 to 176 F Resolution 0 1 Brix 0 190 0 1 F Accuracy 0 2 Brix DIT 0 5 F Temperature Compensation Automatic between 10 and 40 C 50 104 F Measurement Time Approximately 1 5 seconds Minimum Sample Volume 100uL cover prism totally Light Source Yellow LED Sample Cell SS ring and flint glass prism Case Material ABS Enclosure Rating IP 65 Battery Type Life 1 X 9V 5000 readings Auto Off After 3 minutes of non use Dimensions 19 2 W x 10 2 D x 6 7 Hem Mass 420g 3 PRINCIPLE OF OPERATION The Brix determination is made by measuring the refractive index of a solution Refractive Index is an optical characteristic of a substance and the number of dissolved particles in it Refractive Index is defined as the ratio of the speed of light in empty space to the speed of light in the substance A result of this property is that light will bend or change direction when it travels through a substance of different refractive index This is called refraction When passing from a material with a higher to lower refractive index there is a critical angle at which an incoming beam of light can no longer refract but will instead b
9. instrument or prism Battery segment blinking 4S 77 lt 5 of battery life is remaining to solvents that will damage it This includes most organic solvents and extremely hot or cold solutions Temperature values are blinking i Temperature measurement out of 0 0 or 80 0 C Me gas sampling range 0 0 to 80 0 C e Particulate matter in a sample may scratch the prism Absorb sample on soft tissue and rinse sample well with deionized or distilled water between samples e Use plastic pipettes to transfer all solutions Do not use metallic tools such as needles spoons or tweezers as these will scratch the prism Outside temperature compensation ATC segment blinking range 10 to 40 Factory calibration lost SETUP segment blinking Contac Hanna CALIBRATION Calibration should be performed daily before measurements are made when the battery has been replaced or between a long series of measurements 1 Press the ON OFF key then release Two instrument test screens will be displayed briefly a LCD segment followed by the percentage of remaining battery life When LCD displays dashes the instrument is ready m BBBB 100 BBBB BREE c50 2 Using plastic pipettes fill the sample well with distilled or deionized water Note If the ZERO sample is subject to intense light such as sunlight or another strong source cover the sample well with your hand or other shade during the calibration Z
10. rument OFF by pressing the ON OFF key ON e Turn instrument upside down and remove the battery cover by turning it counterclockwise Extract the battery from its location Replace with fresh 9V battery making certain to observe polarity e Insert the back battery cover and fasten it by turning clockwise to engage WARRANTY HI 96801 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions This warranty is limited to repair or replacement free of charge Damage due to accident misuse tampering or lack of prescribed maintenance is not covered If service is required contact your dealer If under warranty report the model number date of purchase serial number and the nature of the failure If the repair is not covered by the warranty you will be notified of the charges incurred If the instrument is to be returned to Hanna Instruments first obtain a Returned Goods Authorization Number from the Customer Service Department and then send it with shipment costs prepaid When shipping any instrument make sure it is properly packaged for complete protection To validate your warranty fill out and return the enclosed warranty card within 14 days from the date of purchase SALES AND TECHNICAL SERVICE CONTACTS Australia Tel 03 9769 0666 Fax 03 9769 0699 China Tel 10 88570068 Fax 10 88570060 Egypt Tel amp
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