BR 12 Manual.indd
Contents
1. Faulty display screen PHD On EHO FDIC AL 2 Contact technical support The cap is not If dangerous or potentially dangerous sample is properly sealed or the _ contained in the tube apply the proper tube is faulty decontamination procedure No display on the screen One or several tubes are not sealed tightly Motor control device damaged due to incorrect voltage selection Return unit to Omni International for replacement of motor control device Unit powers on with a loud BANG Power off the unit and select appropriate voltage Incorrect voltage selected Power on the unit If problem persists contact Omni International for assistance Unit powers on but motor does not turn Speed is too high or Tube leakage High temperature or See solutions on page 13 pressure inside tubes 20 8 3 REMOVING THE IOCKING RING 1 Remove two adjacent 7mL tube holders 2 Unscrew the three set screws around the locking ring using a 050 Allen Wrench 3 Use the 58 to 62 Spanner Wrench provided to loosen the locking ring from the BR12 hub 4 Finish unscrewing the locking ring by hand and remove from the hub 58 to 62 Spanner Wrench ae 050 Allen Wrench 21 9 1 TRANSPORTING THE BEAD RUPTOR 12 The Bead Ruptor 12 must be shipped in its original packaging Before transporting the equipment itis necessary to 1 Close the lid of the Bead Ruptor 12 and cover with the plastic bag 2 Align the slots2. SHOCK Although this eguipment is fully insulated and grounded it is important for all users to be aware of the po tential hazard of using liquids close to a power supply If any liguids are spilled immediately disconnect the instrument from the main power supply remove the power cord from the AC power input on the rear panel and clean the eguipment and the surrounding area DO NOT reconnect the eguipment until it has been fully inspected 2 1 UNPACKING THE BEAD RUPTOR 12 CAUTION DO NOT lift the Bead Ruptor 12 by holding the lid It must be lifted by gripping the sides of the unit and holding it from the bottom 1 Place the crate on a level floor surface 2 Using a screwdriver or power drill remove the 16 screws from the bottom edge of the crate 3 Lift the lid from the crate 4 Lift and remove the inner cardboard box 5 Unscrew the two external knob screws from the back of the unit to release from the support plate 6 Slide the Bead Ruptor away from the knob screw holes 7 Lift the unit up and off of the crate bottom DO NOT LIFT THE UNIT BY HOLDING THE LID 8 Place the Bead Ruptor on a clean horizontal and stable surface and remove the plastic bag PLEASE NOTE Do not discard the crate amp packaging foam This packaging must be used in the event the Omni Bead Ruptor 12 needs to be returned to Omni for any reason SHIPPING THE BEAD RUPTOR IN ANY OTHER PACKAGING WILL VOID ALL WARRANTIES Remove the warning sticke
3. glass beads 0 1mm glass beads 2 38mm metal beads DESCRIPTION ORDER NUMBER Bead Ruptor Tube Sample Pack 19 SAMPLE It is Strongly recommended that only Omni Tubes be used with the Bead Ruptor 12 Other tubes might not be capable of withstanding the forces generated by the Bead Ruptor 12 11 7mL Bead Mill Tubes These Tubes are not pre filled with beads BEAD MEDIA TUBE SIZE 7 0 1mm Empty Sample Tubes with 50 19 654 a Micro Organism Lysing Bead Mix ae 7 0 5mm Empty Sample Tubes with Tough Micro Organism Lysing Bead Mix l Tissue Homogenizing Bead Mix Ceramic l 7 2 8mm Empty Sample Tubes with Hard 50 19 653 el Tene nomogenara enamn PO Metal ae 7 2 38mm Empty Sample Tubes with Hard 19 655 Tissue Grinding Bead Mix mL Bead Tubes are not certified RNase DNase Free and are not currently available pre filled mL mL mL mL mL mL _ Empty Sample Tubes 1 000 19 651 7mL Tube Specifications Treatable Volume e Polypropylene PP e Minimum 1mL 1g e Screw cap tube ONLY e Maximum 5mL 4g e Maximum centrifugation speed of 5 000g e Used with or without buffer It is strongly recommended that Omni 7mL Tubes be used in conjunction with the Bead Ruptor 12 Our tests have shown that Omni Tubes are the only tubes available that are capable of withstanding the forces generated by the Bead Ruptor 12 3 4 IMPORTANT SAMPLE TUBE INFORMATION Dry fresh or frozen samples can be homogenized with or without buffer
4. infringe upon any process patents Notes cautions and warnings within the text of this manual are used to emphasize important and critical instructions This Omni International product is warranted to be free from defects in material and workmanship for a period of ONE YEAR from the date of delivery Omni International will repair or replace and return free of charge any part which is returned to its factory within said period transportation prepaid by user and which is found upon inspection to have been defective in materials or workmanship For the first 90 days both parts and service are without charge For the balance of the period parts will be provided but service will be charged at established labor rates This warranty does not include normal wear from use it does not apply to any instrument or parts which have been altered by anyone other than an employee of Omni International nor to any instrument which has been damaged through accident negligence failure to follow operating instructions the use of electric currents or circuits other than those specified on the plate affixed to the instrument misuse or abuse Omni International reserves the right to change alter modify or improve any of its instruments without any obligation whatever to make corresponding changes to any instrument previously sold or shipped THE FORGOING OBLIGATION IS IN LIEU OF ALL OBLIGATIONS AND LIABILITIES INCLUDING NEGLIGENCE AND ALL WARRANTIES OF MERCHANT
5. safety ground disconnected CAUTION Use only accessories and consumables recommended by the manufacturer Accuracy and operating lifetime may be affected if alternative products are used Any damages caused by non recommended parts are not covered by the manufacturer s warranties 2 2 UNIT OVERVIEW Lid Latch 2mL Tube Holder mL Tube Holder Tube Carriage 120V 220V Switch amp Fuse ON Off Switch Shipping Screw Holes AC Power Input 2 3 COMPONENTS Prior to operation please remove all parts from the shipping container and inspect for damaged or missing parts If any parts are found to be damaged or mising please contact Omni International at 1 800 776 4431 Description Motor Drive Unit 115V or 220V 1 Tube Carriage 12x2mL amp 4x7mL Semi Permanent Ring Spare Fuse Instruction Manual Ouick Start Guide MD Power Cable LT710 115V or LT712 220V 3 1 TUBES TUBE CARRIAGE One Tube Carriage is available for the Bead Ruptor 12 Tube Carriage for 12x2mL 4x7mL screw cap tubes 2mL Tube Volume and Tissue Size Minimum 300uL 30mg Maximum 1 8mL 19 mL Tube Volume and Tissue Size Minimum 1mL 1g Maximum 5mL 4g A maximum volume of 41 6mL can be processed with this tube carriage configuration per run 4x5mL 12x1 8 41 6mL Use the chart on page 10 to select the best tubes for your sample type or to view a selection of Bead Ruptor specific applications visit www omni inc c
6. ABILITY OR OTHERWISE EXPRESSED OR IMPLIED IN FACT OR BY LAW AND STATE OUR ENTIRE AND EXCLUSIVE LIABILITY AND BUYERS EXCLUSIVE REMEDY FOR ANY CLAIM OF DAMAGES IN CONNECTION WITH THE SALE OR FURNISHING OF GOODS OR PARTS THEIR DESIGN SUITABILITY FOR USE INSTALLATION OR OPERATION OMNI INTERNATIONAL WILL IN NO EVENT BE LIABLE FOR ANY SPECIAL OR CONSEOUENTIAL DAMAGES WHATSOEVER AND THEIR LIABILITY UNDER NO CIRCUMSTANCES WILL EXCEED THE CONTRACT PRICE FOR THE GOODS FOR WHICH LIABILITY IS CLAIMED READ ALL INSTRUCTIONS BEFORE USING SAVE THIS USER MANUAL The Bead Ruptor 12 has been engineered for maximum functionality as well as safety however basic safety precautions and common sense must always be demonstrated when using any electrical product Do not attempt to modify any part of the Bead Ruptor 12 If you experience problems with or have questions about your Bead Ruptor 12 contact your authorized dealer or call Omni at 1 800 776 4431 or 770 421 0058 WARNING DO NOT allow the machine to be submerged in any liguid DO NOT use in any setting other than an indoor laboratory DO NOT plug power cord into an incorrect outlet Keep this product away from heated surfaces To reduce the risk of burns electrocution fire or injury Use this product only for its intended purpose as described in this booklet Do not use attachments not recommended by the manufacturer DO NOT operate the product if it is damaged in any way RISK OF ELECTRIC
7. L INC v v ES The user can adjust two different homogenization settings with this interface The UP arrow and DOWN arrow buttons are used to adjust the speed and time parameters 4 3 START UP OF THE EQUIPMENT Turn the Bead Ruptor 12 on by pressing the ON OFF switch located on the rear panel near the AC power input A Menu displays the programming mode Programming mode displays the last program run before power was removed Example Cycle Time di TIME sec 35 SPEED HIGH Speed Setting Low 3m s Med 4m s System Status High 5m s e Speed denotes energy imparted to the samples in m s and can be set at 3m s low 4m s med or 5m s high Time denotes the processing time in seconds Time can be set from 0 01 seconds to 0 99 seconds in 1 second increments 4 4 SPEED SETTING Homogenization with the Bead Ruptor 12 is the result of the collision of the bead matrix and sample within the Bead Ruptor tubes The frequency of collision and energy of impact determine the effectiveness of the disruption process and are controlled by the Bead Ruptor 12 speed setting the motion of the tube carriage the bead media and the tube fill volume The Speed of the Bead Ruptor is presented in m s which is a specific representation of the forces applied to the sample due to the velocity ofthe tube carriage movement and the speed and geometry ofthe motion Using RPM for example is not representative of the bead movement a
8. aned with a sponge or a damp cloth moistened with a non abrasive cleaner In case another decontamination procedure is to be applied please first contact technical support to ensure the compatibility of the new procedure with the instrument 19 DO NOT attempt to service the Bead Ruptor 12 in a manner other than those discussed in this manual For any issue that is unsuccessfully corrected using this guide please contact your authorized dealer or call Omni International at 800 776 4431 8 1 ERROR MESSAGES Possible Causes Action s Close Door i 1 Check that nothing prevents the lid from closing NEE IA a AOpen 2 Press lid and ensure the handle is locked properly 1 Turn off the unit PONS HI 2 Contact technical assistance 8 2 TROUBLESHOOTING Possible Causes 1 Check main voltage 2 Check that the voltage of the unit matches that No Power being delivered by the main power supply 3 Make sure that the unit is plugged in properly 4 Check and replace fuses if blown Wn 1 Turn off the unit i 2 Contact technical support 1 Turn off the unit Wait a few seconds Turn the unit back on The fan does not work Display screen shows incomplete or CPU needs to be reset unusual characters 1 Check main voltage 2 Check that the voltage of the unit matches that No Power being delivered by the main power supply 3 Make sure the unit is plugged in properly 4 Check and replace fuses if blown
9. ead Ruptor 12 User Manua WARRANTY INFORMATION SECTION 1 IMPORTANT SAFEGUARDS SECTION 2 UNPACKING THE BEAD RUPTOR 12 2 1 Unpacking The Bead Ruptor 12 2 2 Unit Overview 2 3 Components 2 4 Additional Accessories SECTION 3 BEAD RUPTOR SAMPLE TUBES 3 1 Tubes amp Tube Carriage 3 2 Tube Selection Guide 3 3 Ordering Information 3 4 Important Sample Tube Information 3 5 Tube Carriage Chart SECTION 4 SET UP amp OPERATION 4 1 Loading Sample Tubes 4 2 Keypad Layout 4 3 Start Up of the Equipment 4 4 Speed Setting SECTION 5 PROGRAMMING 5 1 Programming SECTION 6 RECOMMENDED SETTINGS SECTION 7 MAINTENANCE 7 1 Cleaning The Bead Ruptor 12 Section 8 Troubleshooting 8 1 Error Messages 8 2 Troubleshooting SECTION 9 TRANSPORT STORAGE amp SERVICE 9 1 Transporting The Bead Ruptor 24 9 2 Storage 9 3 Service 9 4 Decontamination Requirement SECTION 10 SPECIFICATIONS This manual is a guide for the use of the Bead Ruptor 12 Bead Mill Homogenizing System and accessories Data herein has been verified and validated It is believed adequate for the intended use of the instrument If the instrument or procedures are used for purposes over and above the capabilities specified herein confirmation of the validity and suitability should be obtained otherwise Omni International does not guarantee results and assumes no obligation or liability This publication is not a license to operate under or a recommendation to
10. estions regardsing your application please call Omni Technical support at 1 800 776 4431 BEAD MATRIX SETTING APPLICATION cycle x time pause Bacteria Muscle Brain Liver ANIMAL Kidney Lung Heart Spleen Leaves Leaves Seeds Stems Stems Roots Roots Flowers 19 628 High 2 x 45 min RNA Isolation sol Soil 19 624 DNA Isolation Our Applications database can help you find the best solution quickly Our constantly expanding database consists of carefully reviewed customer published applications to help you find the right laboratory homogenizer for any sample type www omni inc com applications 18 7 1 CLEANING THE BEAD RUPTOR 12 The houseing of the unit can be cleaned with a sponge or damp cloth moistened with water or alcohol WARNING For safety purposes and to prevent any damage of the unit the recommendations listed below should be strictly followed ALWAYS disconnect the power cord before cleaning DO NOT spray water directly on the unit expecially in the air vents and the fan DO NOT use any type of scrapers DO NOT use caustic solvents or acetone Harsh solvents can damage the finish of the housing and lid Example of Decontamination Procedure If a tube breaks when a run is in progress decontaminate parts that may have been contaminated withan appropriate disinfectant The decontamination procedure is the sole responsibility of the user Parts that may have been contaminated can be cle
11. nd processing energy because RPM only indicates the motor speed 5 1 PROGRAMMING The Bead Ruptor 12 is programmable for Speed Processing Energy and Time The Bead Ruptor memory will store the last program settings used After powering on the LCD screen will display the last used speed and time settings The UP and DOWN arrow keys respectivly increase or decrease the value of the active variable speed or time by holding down the arrow button 12 SIDE TIME OMNI TERNATIONAL INC M M ES Active Variable System Status Display Speed SPEED m s Time CYCLE TIME sec Operating Range Speed Energy Low Medium High From 1 second to 99 seconds Storable Programs e If run is stopped or paused system status will read RUN CANCELLED At the end of the program RUN COMPLETE will be displayed CAUTION Wait until the unit stops completely and displays RUN COMPLETE before opening the lid PLEASE NOTE e Homogenization settings cannot be changed once the run is in progress e The Bead Ruptor 12 is designed for non stop use and does not require a cool down period between runs The user can pause or stop a run by pressing the STOP button at any time 17 Please Note These protocols are provided as a basic guidline only the listed settings might need to be modified for your particular sample type viscosity weight volume cell concentration and other factors that effect homogenization If you have any qu
12. nstrument or parts being returned If no Decontamination Certificate is attached and a potential radioactive or biological hazard is detected or suspected by Omni International the equipment will not be serviced until proper decontamination and certification is complete The sender will be contacted for instructions as to the dispostion of the equipment Disposition costs will be borne by the sender WARNING Itis a violation of federal law to transport biologically hazardous or radioactive materials without proper packaging labeling and appropriate warnings 23 10 1 SPECIFICATIONS TT EYES Height EEE YETT Weight 44S 17kg S Energy Performance Range 3m s 4m s 5m s Low Med High lt 70db Electrical Requirements 115 volts 60Hz or 220 volts 50Hz Standards Approval Compliance CE certified Register your Bead Ruptor amp tell us about your research Please visit the web address below to register your new Bead Ruptor You will also be asked to provide us with information about your re search In return we ll send you a Starbucks gift card Register today www omni inc com warranty 24 LA N N N N OMNI INTERNATIONAL C omni inc com 935 C Cobb Place Blvd Kennesaw GA 30144 Toll Free 800 776 4431 Phone 770 421 0058 Patents Pending PN 03 25 1 28
13. om applications 3 2 TUBE SELECTION GUIDE LYSE DISRUPT RNA HOMOGENIZE GRIND MICROBIAL Bacteria V Y Y XV o _ Yeast 47 YY Fungi Y 47 4 4 o po o Spores Yo 4 4 4 ANIMAL Skin tt V EZ EEE v m Nees KA s po Artery hh Intestine KA OK Tumors KA xy x Brain KA KA ys tiver Kidney tug Heat FISH INSECTS 3 3 ORDERING INFORMATION 2mL amp 0 5mL PRE FILLED BEAD MILL SAMPLE TUBES TUBE BEAD DESCRIPTION QUANTITY DNAse RNAse UNTREATED SIZE SIZE FREE PART PARTH Mix Glass 2mL 0 5mm _ Tough Micro Organism 50 19 622 19 622C mm gw IO 9 IO Organism Lysing Mix Garnet 2mL 0 7mm _ Hard amp Fibrous Tissue 50 19 624 19 624C 0 5mL 0 25mm RNA Extractions 50 19 625 N A Carbide Micro Organism amp Soil Homogenizing Mix 2mL 1 4mm Soft Tissue 50 19 627 19 627C Homogenizing Mix mL 0 2 8mm Hard Tissue 19 628 19 628C Homogenizing Mix Hard Tissue Grinding Mix 19 620 19 620C Untreated Tubes are clean but not certified DNAse or RNAse free ni Bulk Sample Tubes No Beads Empty sample tubes with caps and O Rings Beads are not included 00 mL Empty Sample Tubes with Caps 1000 19 649 Empty Sample Tubes with Caps 19 650 Bead Ruptor Tube Sample Pack Includes 4 each of the following pre filled sample tubes 1 4mm ceramic beads 2 8mm ceramic beads 0 5mm
14. on the bottom of the unit with the support hooks on the base of the shipping crate 3 Lower the unit onto the hooks and slide it backward until the back of the unit touches the supporting edge 4 Secure the unit to the support plate with the included knob screws Tighten the screws as much as possible 5 Place the cardboard box over the Bead Ruptor 6 Use a screwdriver or power drill to secure the lid to the top of the crate with 16 screws 22 PLEASE NOTE The Bead Ruptor 12 MUST be shipped in its original packaging Failure to do so can result in serious damage to the unit and will void all warranties 9 2 STORAGE The unit can be used in a cold room but it must be stored in a dry area at a temperature ranging from 0 C 32 F to 50 C 122 F 9 3 SERVICE It is recommended that the speed of the Bead Ruptor 12 be calibrated annually to ensure maximum operation For this or any other product servicing please contact Omni International at 800 776 4431 9 4 DECONTAMINATION REQUIREMENT Should an instrument or component that has been used with radioactive or pathogenic material require factory or field service comply with the following procedure to ensure the saftey of service personnel Clean the parts to be serviced of all encrusted material and decontaminate them There must be no radioactivity detectable by survey equipment Obtain a decontamination Certificate from Omni International Complete the certificate and attach to the i
15. r from the back of the unit and check that the voltage switch matches the voltage delivered by your electric power supply CAUTION If the voltage supplied to the eguipment is higher than what is indicated on the voltage switch the electronics can be irreversibly damaged voiding the warranty Ensure that the air vents are not covered Allow at least 6 inches of space around air vents for proper motor ventilation CAUTION DO NOT connect the unit to the main power supply until installation is complete and the correct voltage is selected Plug the Bead Ruptor 12 into the power supply using the supplied power cord In the event of a malfunction or breakdown grounding provides a path of least resistance for electric current to reduce the risk of electric shock This instrument is eguipped with an electric cord which is grounded to the chassis housing The plug must be plugged in to an outlet that is properly installed and grounded in accordance with all local codes and ordinances WARNING DO NOT modify the plug or cord that is provided Damaged or worn power cords should be repaired or replaced immediately by a gualified electrician DO NOT turn the unit upside down the Bead Ruptor 12 must always rest on all four feet to avoid damaging internal components or breaking the housing DO NOT operate the unit when the housing is removed potentially lethal voltage exists within the instrument DO NOT operate the unit with the
16. using the Bead Ruptor 12 and Bead Ruptor sample tubes Omni offers unique reinforced 2mL tubes with an O ring seal in the cap These tubes can withstand the motion velocity and force applied to the tube carriage In the event of tube leakage or tube breakage Omni recommends the following solutions e Use the Tubes as single use or disposable Do not submerge the tubes in liquid nitrogen Add more buffer to decrease the force applied by the beads to the tube walls Ensure tube caps are tightened completely e If using bulk beads and tubes add the recommended amount of beads to the tube 4 1 LOADING SAMPLE TUBES Users can simultaneously homogenize 1 to 16 samples per run The user may load tubes in any position for processing DO NOT RUN AN EMPTY TUBE CARRIAGE IDENTIFICATION OF PARTS Tube Carriage ap 2mL Tube Holder 2 Semi Permanent Locking Ring CAUTION The drive hub can become very hot during operation Use caution when removing tubes from the Tube Carriage Tube Holders Lid Locking Handle Note 7mL tubes are inserted upside down unlike the 2mL tubes which go in right side up 1 Place Bead Ruptor Tubes into the Tube Holders Drive Hub 2 Close the lid until the locking lid handle clicks 14 4 2 KEYPAD LAYOUT The keypad of the Bead Ruptor 12 see diagram below consists of an LCD screen 4 arrow buttons and a START and STOP button 12 DD TIME OMNI INTERNATIONA
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