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User`s Manual

1. Freeze Dryers Type Model 775 followed by two digit number based on model size followed by another two digit number based on options 77400 followed by two digit number based on options 79340 followed by two digit number based on options 767 followed by two digit number based on model size followed by another two digit number based on options 79600 followed by two digit number based on options 74200 followed by two digit number based on options 738 followed by two digit number based on model size followed by another two digit number based on options Manufacturer Labconco Corporation 8811 Prospect Avenue Kansas City MO 64132 USA Ph 816 333 8811 Fax 816 363 0130 labconco labconco com is in conformity with the standards listed below EN61010 1 EN61326 1 EN55022 EN61000 3 2 3 following the provisions of the following directives 89 336 EEC 2002 95 EC RoHS 2004 108 EC 2006 95 EC when installed and operated in accordance with the manufacturers installation and operating instructions Krusa Cry AO USA lt 3 3Bl 2001 Mark Schmitz Place and date of issue Nee President Research and Engineering Labconco Corporation Labconco P N 3696002 Rev H 56 Product Service 816 333 8811 or 1 800 522 7658
2. User s Manual FreeZone Plus 4 5 Liter Cascade Freeze Dry Systems Models 73820 Series 73860 Series 73870 Series To receive important product updates complete your product registration card online at register labconco com Protecting your laboratory environment 7 LABCONCO Labconco Corporation 8811 Prospect Avenue Kansas City MO 64132 2696 800 821 5525 816 333 8811 FAX 816 363 0130 E MAIL labconco labconco com HOME PAGE www labconco com Please read the User s Manual before operating the equipment Copyright 2008 2009 2012 Labconco Corporation All rights reserved The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change Warranty Labconco provides a warranty on all parts and factory workmanship The warranty includes areas of defective material and workmanship provided such defect results from normal and proper use of the equipment The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco whichever is sooner except the following e Purifier Logic Biosafety Safety Cabinets and PuriCare Lab Animal Research Stations carry a three year warranty from date of installation or four years f
3. ACCESSORIES The following accessories are available for the freeze dryer PART DESCRIPTION 1472100 Vacuum Pump Two stage direct drive pump 117 liters minute 115 VAC 50 60 Hz single phase 4 6 amps Includes Pump Exhaust Filter 1473400 Cat 1473400 7739402 Vacuum Pump Two stage direct drive pump 117 liters minute 230 VAC 50 60 Hz single phase 2 4 amps Includes Pump Exhaust Filter 1473400 Cat 1473400 1467700 Vacuum Pump Two stage direct drive pump 195 liters minute 115 VAC 50 60 Hz single phase Includes Pump Exhaust Filter Cat 1473400 7739403 Vacuum Pump Two stage direct drive pump 195 liters minute 230 VAC 50 60 Hz single phase Includes Pump Exhaust Filter Cat 1473400 7584000 Vacuum Pump Chemical Resistant Hybrid Combination two stage rotary vane pump and two stage chemistry diaphragm pump 98 115 liters minute 115 VAC 50 60 Hz single phase 7584002 Vacuum Pump Chemical Resistant Hybrid Combination two stage rotary vane pump and two stage chemistry diaphragm pump 98 115 liters minute 230 VAC 50 60 Hz single phase Product Service 816 333 8811 or 1 800 522 7658 49 Appendix D Freeze Dryer Accessories PART DESCRIPTION 1472200 Pump Inlet Filter Disposable filter that prevents oil back streaming and protects vacuum pump from submicron particles Fits Vacuum Pumps 1472100 and 7739402 1473400 Pump Exhaust Filter Disposable filter that removes visible oil mist and odor f
4. ss v 5 x 4 3 amp 2 a H 3 N mn s 2 2 a Ss 5 g OD R YG lt 2 amp gt T a E g 2 Z 8 23 Z B Z 5 S 2 lt s 2 5 5 gt oD u D Q a S S Q Q oo Pa Component Material lt amp amp D n is a e Valve Stem Acetal Delrin C D D D D Collector Lid Acrylic D i D D D Hoses Gaskets Neoprene C D D C D D amp Valve Bodies Flask Top Silicon Rubber C D D D D Chamber amp Stainless Steel C Fittings C Moderate degradation Limited use D Severe degradation infrequent use recommended immediate thorough cleaning required e Most common compounds used in freeze drying processes if allowed to enter the vacuum pump will degrade the oil and cause damage to the vacuum pump e Sugars and proteins typically will have minimal negative effect on any of the materials of construction When using compounds in the freeze dryer that are hostile to the materials of construction it is imperative the equipment is thoroughly cleaned after use e Rubber and plastic components that have been exposed to damaging compounds should be removed and flushed with water 12 Product Service 816 333 8811 or 1 800 522 7658 Dioxane Methyl t Butyl Ether BTBE Pyridine J Chapter 3 Getting Started e The oil in the vacuum pump should be checked often It must be changed if it is cloudy shows particles or is discolored The useful life of vacuum pump oil can be exte
5. 30 60 300 or 600 second intervals e Press SELECT to return to display operating parameters or after a short delay the display will automatically switch to show operating parameters Automatic Start Up To run the Auto Mode press the panel switch labeled REFRIGERATION AUTO The green LED above the switch illuminates and the refrigeration system starts When the collector reaches 40 C the vacuum pump will start The Temperature and Vacuum Graph indicate collector temperature and system vacuum The LCD display shows the actual temperature of the collector When the vacuum in the system 1s above 5 mBar the vacuum display indicates HI At 5 mBar and below the display shows the actual vacuum When the system vacuum is between 0 450 and 0 133 mBar the lower green vacuum graph LED will flash When the system vacuum level is lt 0 133 mBar the green LED will be lit steadily indicating that most samples may be added Manual Start Up To manually run the freeze dry process press the REFRIGERATION MAN switch This starts the refrigeration system The green LED above the switch illuminates When the collector temperature reaches 40 C the vacuum pump may be started by pressing the VACUUM switch The Temperature and Vacuum Graph indicate collector temperature and system vacuum The LCD display shows the actual temperature of the collector When the vacuum in the system is above 5 mBar the vacuum display indicates HI At 5 mB
6. 522 7658 APPENDIX B FREEZE DRYER DIMENSIONS Model 73860xx INCHES CENTIMETERS 2295 44 Product Service 816 333 8811 or 1 800 522 7658 Appendix B Freeze Dryer Dimensions Model 73870xx ENTIMETERS gt k Ir ii i 0000000 UDAL DUO DO0UOU DOU DOU DOU OOU NOL OODQO0OD BANDON ANA MONONA AL OONOODOATOAIAAASAAGABAN NMN DUAHANABNOANOTOOLAQAHAUOMI DO DOU IND OO0 DOU OO0 000000000 DODOOIUDIOODODIOOOUOIOODUI ka m CY 19 3 Bae Thee 45 Product Service 816 333 8811 or 1 800 522 7658 Appendix B Freeze Dryer Dimensions Model 73820xx INCHES CENTIMETERS I es o os I cry ce SS co Ce 46 Product Service 816 333 8811 or 1 800 522 7658 APPENDIX C FREEZE DRYER SPECIFICATIONS This Appendix contains technical information about the freeze dryer including electrical specifications environmental operating conditions and wiring diagrams Electrical Specifications Catalog minal l oma Max with Operating w o Nominal Vacuum Range Vacuum Pump Pump 7386020 115V 103 127 60 1 u i6 jp 1oy sono so J i p u te 7386021 115V 103 127 6 1 u 16 100v 90 110 50 1 u l6 7386040 230V 187 253 60 1 55 l0 7386041 230V 187 253 60 1 55 10 a ES a 7387020 MSV 103 127 60 1 it e 100v 90 110 50 1 u 16 7387021 115V 103 127 60 1 u l6 pt toov
7. DRYING CHAMBER NOT INSTALLED Press and hold SELECT to change the status Press MENU and the display shows PURGE VALVE NOT INSTALLED Press and hold SELECT to change the status Press MENU and the display shows DEFROST HEATER NOT INSTALLED Press and hold SELECT to change the status Press MENU and the display shows MOISTURE SENSOR INSTALLED Press and hold SELECT if you want to disable the moisture sensor Press MENU and the display shows SHELL FREEZER NOT INSTALLED Press and hold SELECT to change the status Press MENU and the display shows HOLD SELECT TO CLEAR ALL TOTAL HOURS If you want to reset Total Hours press and hold SELECT until an audible beep is heard The display defaults to the main freeze dry display information If you do not want to set Total Hours press MENU and the display shows PRODUCTION MENU MASTER RESET 40 Product Service 816 333 8811 or 1 800 522 7658 Chapter 8 Modifying Your Freeze Dryer Display The routine repeats by pressing MENU or wait approximately 10 seconds for the display to default to the main freeze dry information display information In order to store offsets in memory you Must Press and Hold MENU until display shows PASSWORD Wait approximately 10 seconds and the display defaults to the main freeze dry display information This can be done anytime throughout the Modifying the Display routine Product Service 816 333 8811 or 1 800
8. It is shipped enabled as the factory default setting Modifying the Display To enter an offset or to configure the alarms perform the following steps Press and hold MENU button approximately 10 seconds until the display shows PRODUCTION MENU MASTER RESET It is recommended that you do NOT perform a Master Reset because all offsets and configurations that have been entered will be lost If you must perform a Master Reset hold SELECT approximately 5 seconds This will reset Vacuum units to mBar Vacuum set point to 0 mBar Temperature units to C Refrigeration total hours to 0 Vacuum pump total hours to 0 RS232 transmission rate to 10 seconds Temperature offset to 0 C Vacuum offset to 0 mBar Low line voltage to 0 V offset High line voltage to 0 V offset Moisture sensor to enabled Refrigeration to operate in Manual Mode The display will default to the main freeze dry display information If you do not want to restore all factory settings but want to adjust offsets press MENU briefly and the display shows COLLECTOR TEMP OFFSET OC Press SELECT until the appropriate offset is shown Each number represents 1 C 38 Product Service 816 333 8811 or 1 800 522 7658 Chapter 8 Modifying Your Freeze Dryer Display Press MENU and if the drying chamber has been installed the display shows SDC TEMP OFFSET OC Press SELECT until the appropriate offset is shown Each number represents 1 C Press MENU and
9. K 901o N S50 J i Wo ao yo Te 7387040 230V 187 253 60 1 55 10 rasroat 20v 187 253 6o f i f ss f oo Ene ve ee E E E 7387061 230V 198 254 5o 1 55 10 B8070 20v 198254 S0 f 1 55S 10 198 254 La o a y e Product Service 816 333 8811 or 1 800 522 7658 47 Appendix C Freeze Dryer Specifications Catalog boas Nowal Max with Nominal P 8 Vacuum Vacuum 7382020 115V 103 127 60 7382021 115V 103 127 60 1 Environmental Conditions 48 Indoor use only Maximum altitude 6562 feet 2000 meters Ambient temperature range 41 to 104 F 5 to 40 C Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C Main supply voltage fluctuations not to exceed 10 of the nominal voltage Transient overvoltages according to Installation Categories II Overvoltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed Used in an environment of Pollution degrees 2 1 e where normally only non conductive atmospheres are present Occasionally however a temporary conductivity caused by condensation must be expected in accordance with IEC 664 Heat reyected from freeze dyer excluding the vacuum pump Less than 4320 BTU HR Product Service 816 333 8811 or 1 800 522 7658 AppPENDIx D FREEZE DRYER
10. high stage system refrigerates the condenser of the low stage system This takes place in the heat exchanger The low stage and high stage systems are balanced by design however sometimes that balance can be disrupted during operation and the unit will not function properly If the unit is overloaded or if the unit is defrosted and restarted in a short time interval system unbalance is possible If system unbalance occurs turn the freeze dryer off and allow the unit to stabilize at ambient temperature for 2 to 4 hours If any repairs are required on the refrigeration module contact your local laboratory supply dealer If your laboratory supply dealer is unable to provide assistance please call Labconco Repairs should only be undertaken by a refrigeration technician or through an authorized Labconco service agency 36 Product Service 816 333 8811 or 1 800 522 7658 CHAPTER 8 MobDIFYING YOUR FREEZE DRYER DISPLAY Your freeze dryer has been carefully calibrated and tested before shipping however under certain circumstances it may be necessary to adjust the calibration of the temperature and or vacuum display or to modify alarm points Adjustments may be necessary if the freeze dryer requires service or if it is operating on a line voltage outside the voltage range at which the alarm was preset This DOES NOT mean that the freeze dryer may be operated at a voltage outside the ranges shown in Appendix C Temperature and Vacuum
11. in nine convenient Sizes Flat bottom No threads reduces spills hooks or springs provides better are needed balance Entire flask may be autoclaved Adapters Add the adapters for connecting the Fast Freeze Flasks to the valve ports on your drying chamber or manifold Choose borosilicate glass or stainless steel adapters in 72 and diameters Flask Complete Flask Flask krasy Top Dimensions Size Flask Bottom Top Adapter mash BONOm Diameter H x D 40 ml 7540000 7542000 7544000 VoM 76mm 34mm 80 ml 7540200 7542200 7544000 Yas 115mm x 34mm 120 ml 7540300 7542300 7544200 y4 68mm x 59 2mm 150 ml 7540400 7542400 7544200 Ya 85mm x 59 2mm 300 ml 7540600 7542600 7544200 Ya 145mm x 59 2mm 600 ml 7540800 7542800 7544400 34 135mm x 90 2mm 750 ml 7541100 7542700 754400 34 162mm x 90 2mm 900 ml 7540900 7542900 7544400 34 190mm x 90 2mm 1200 ml 7541000 7543000 7544400 y4 240mm x 90 2mm 2000 ml 7541200 7543200 7544400 y4 380mm x 90 2mm Valves on the freeze dry system drying chambers and manifolds offered in this catalog fit adapters of both 2 and 3 4 sizes Size information is provided for selecting adapters for older Labconco models and other freeze dryers 7544810 Replacement Filter Paper 1000 10 packages of 100 1 diameter 28mm Pore size 17 micron Shipping weight 0 5lb Appendix D Freeze Dryer Accessories Lyph Lock Flasks Borosilicate glass Compatible with adapters with i all major ei
12. in vapor pressure and ultimately overloads the collector and vacuum pump Samples that have eutectic temperatures of 20 C or lower should be placed on the freeze dry system one flask at a time so that the vacuum in the system may recover before adding another sample to the system If the vacuum does not recover the capacity of the freeze dry system has been exceeded and the sample should be removed Product Service 816 333 8811 or 1 800 522 7658 3 Chapter 1 Introduction If there 1s a problem with a particular type of sample melting when placed on the freeze dry system dilution of the sample with more water or providing some insulation around the flask to decrease the rate of heat absorption by the sample may help If the eutectic temperature of the sample is 40 to 60 C the freeze dry system selected for use must be equipped with cascade type refrigeration so that the collector temperature can be cooled to below 75 C or a dry ice solvent trap may be used between the collector and the vacuum pump Samples Containing Volatile Substances In certain cases the solvent in a sample to be freeze dried may contain volatile components such as acetonitrile methanol acetic acid formic acid or pyridine In addition to these substances having an effect on the eutectic temperature they may increase the vapor pressure at the surface of the sample Also compared to water they will require the absorption of less heat for sublimation
13. the display shows VACUUM OFFSET O uBar Press SELECT until the appropriate offset is shown Each number represents 0 001mBar Press MENU and the display shows LOW LINE OFFSET 0 Press SELECT until the appropriate offset is shown If the freeze dryer is operating on nominal 115V each number represents 2V If 3 is entered into the display the low line alarm activates when the voltage drops to approximately 97V for example 103 2x3 97V If the Freeze Dryer is operating on nominal 230V each number represents 4V if 3 is entered into the display the low line alarm activates when the voltage drops to approximately 194V for example 206 4x3 194V There will be no alarm indicated if DISABLED 1s selected Press MENU and the display will show HIGH LINE OFFSET 0 Press SELECT until the appropriate offset is shown If the freeze dryer is operating on nominal 115V each number represents 2V If 3 is entered into the display the high line alarm activates when the voltage raises to approximately 133V for example 127 2x3 133V Ifthe Freeze Dryer is operating on nominal 230V each number represents 4V if 3 is entered into the display the low line alarm activates when the voltage drops to approximately 266V for example 254 4x3 266V There will be no alarm indicated if DISABLED is selected Product Service 816 333 8811 or 1 800 522 7658 39 Chapter 8 Modifying Your Freeze Dryer Display Press MENU and the display shows
14. tight and sealed properly against the chamber Inspect valves for cracks or cuts Inspect gasket s for cracks or defects Ensure the gasket is clean Apply a thin coat of vacuum grease to the gasket Keep in mind too much vacuum grease can actually cause leaks Check the drain line and ensure the drain plug is installed and the tube appears to be in good condition Check all rubber vacuum tubing for signs of deterioration or cracking Check all connections and make sure they are secure and leak tight When checking the sample valves pull as much vacuum as possible If a vacuum indication is displayed wiggle or rotate the valves and watch the gauge for any fluctuations Fluctuations can indicate a potential vacuum leak If the valve seems to be in good condition remove the valve and apply a thin coat of vacuum grease to the stem and the outside sealing surface of the valve body and reinstall the valve DO NOT APPLY VACUUM GREASE TO THE SAMPLE VALVE GASKETS WHICH SEAL TO THE WALL OF THE DRYING CHAMBER If the valve still seems to be the source of the problem remove the valve and stopper the hole with a rubber stopper Continue checking the other valves Make sure that the sample valve bulkhead nuts are tight Either tighten by hand or use the wrench supplied with chambers To use the wrench first remove the knob and stem assembly and also the valve body The pins on the wrench fit into the round holes of the nut The nut should be snug but a
15. x x Plus the following Power Cords Chamber Lid If you did not receive one or more of the components listed for your freeze dryer or if any of the components are damaged contact Labconco Corporation immediately for further instructions 10 Product Service 816 333 8811 or 1 800 522 7658 Chapter 3 Getting Started Setting Up Your Freeze Dryer After you verify receipt of the proper components move your freeze dryer to the location where you want to install it Then follow the steps listed below Vacuum Pump Connection A vacuum pump as described in Chapter 2 Prerequisites 1s required to operate your freeze dry system properly The freeze dryer is equipped with a 3 4 ID heavy wall vacuum hose for connecting the collector chamber to the vacuum pump Place the vacuum pump near the benchtop freeze dryer models For console models from the back of the unit place the vacuum pump on the floor of the cabinet Connect the vacuum pump power cord to the receptacle on the back of the cabinet labeled vacuum pump If the vacuum pump has an off on switch turn the switch on The vacuum pump will be controlled by the freeze dryer Connect the vacuum pump inlet port to the vacuum tube on the back of the cabinet with the vacuum hose and clamps provided If necessary cut the hose to proper length to allow for gentle bends without kinks Models rated for use on 230V have a reverse IEC connector for attaching the va
16. 21 C and at this temperature the vapor pressure is about 1 16 that of water at 0 C Although the eutectic temperature is not dependent upon the concentration of sodium chloride the vapor pressure of the water would decrease as the concentration of sodium chloride increased This is due to the fact that as the solute concentration increases less of the surface area of the frozen sample is occupied by water In general most solutions or biological samples will have a eutectic temperature of 10 to 25 C However if the sample contains a simple sugar such as glucose or 1f the sample is animal or plant tissue the eutectic temperature may be as low as 30 to SO0 C Freeze Dry Capacity The volume of a sample that can be freeze dried at one time is related to factors discussed previously and the size and design of the freeze dry system With any given instrument the capacity is based on the surface area of the sample the eutectic temperature and concentration of the sample and the rate and amount of heat transferred to the frozen sample Of these factors the eutectic temperature is the most important factor in determining the amount of sample that can be freeze dried at one time particularly when flasks are used This is because as the eutectic temperature decreases the vapor pressure decreases but the rate of heat absorption by the sample does not change This tends to promote melting of the sample which leads to a marked increase
17. 522 7658 41 APPENDIX A FREEZE DRYER COMPONENTS The following is a list of components that are available for your freeze dryer The parts shown are the most common replacement parts If other parts are required contact Product Service en a 73860xx 73870xx 73820xx 7388401 7383601 Back Panel Assembly 230V00 o P 7406601 7383501 Motor Fan 230V 00Hz _ _ _ f 7406602 7383502 Motor Fan 230V 50Hz _ _ _ 7591806 7382101 Compressor 230V 50 Hz Lo Stage f 7591807 7382102 Compressor 230V 60 Hz Lo Stage f 7591805 7382100 Compressor 115V 60 Hz Hi Stage f 7591806 7382101 Compressor 230V 50 Hz Hi Stage _ 7591807 7382102 Compressor 230V 60 Hz Hi Stage 6 f 1302300 1302300 Switch gt S O 8 7445500 7445500 Printed Circuit Board lt 9 7728000 7728000 DrainPug S O f 1342100 1342100 Power Cord 230V 60 Hz Not Shown NEMA 6 20 f 1336500 1336500 Power Cord 230V 50 Hz Not Shown CEE 7 7 Ff 1332601 1332601 Power Cord 230V 50 Hz Not Shown BS 1363 P 1332701 1832701 Power Cord 230V 50 Hz Not Shown CH2 16P_ 7389403 7382301 Wire Harness 230V Not Shown Wire Harness Control 115V amp 230V Not Shown 42 Product Service 816 333 8811 or 1 800 522 7658 Appendix A Freeze Dryer Components Models 73860xx and 73870xx rh B s B O e i i 43 Product Service 816 333 8811 or 1 800
18. Display An offset may be added to the collector temperature or vacuum display Use an accurate traceable known reference to compare the readings Temperature offset may be varied 5 C in 1 C increments Vacuum offset may be varied 0 020 mBar in 0 001 mBar increments Line Voltage Alarm Points The freeze dryer is designed to alarm if the line voltage varies more than 10 from nominal line voltage Models rated to operate on 115V are preset to alarm if the voltage drops below approximately 103V or rises above approximately 127V Models rated to operate on 230V will alarm if the voltage drops below approximately 206V or rises above approximately 254V Some models are designed to operate below these ranges see Appendix C In these cases it is permissible to lower the low voltage alarm point to prevent nuisance alarms from occurring Low line limit may be lowered in 5 increments of approximately 2 to 3V increments for 115V models or disabled Low line limit may be lowered in 5 increments of approximately 4 to SV increments for 230V models or disabled High line limit may be raised in 5 increments of approximately 2 to 3V increments for 115V models or disabled Product Service 816 333 8811 or 1 800 522 7658 37 Chapter 8 Modifying Your Freeze Dryer Display High line limit may be raised in 5 increments of approximately 4 to 5V increments for 230V models or disabled Moisture Sensor Alarm The moisture sensor alarm may be disabled
19. Drying Manifold Installation Chemical Resistance of Freeze Dryer Components Solvent Safety Precautions CHAPTER 4 USING YOUR FREEZE DRYER Freeze Dryer Controls Operation Checklist Vacuum Pump Ballast Setting Operating the Freeze Dryer Set Up Automatic Start Up Manual Start Up Setting the Operating Vacuum Level Pre Freezing Samples Adding Sample Shut Down Defrosting BK BR WN Re NNUNU AA DW Alarms Power Failure Line Voltage Out of Range Temperature Out of Range Service Vacuum Pump Moisture in Collector CHAPTER 5 MAINTAINING YOUR FREEZE DRYER CHAPTER 6 USING THE RS232 RECEPTACLE Computer Connection for Computer Interface CHAPTER 7 TROUBLESHOOTING Vacuum Pump Gaskets Tubing Connections Sample Valves System Components amp Collection Chamber Isolation Refrigeration Module Operation CHAPTER 8 MODIFYING YOUR FREEZE DRYER DISPLAY Temperature and Vacuum Display Line Voltage Alarm Points Moisture Sensor Alarm Modifying the Display APPENDIX A FREEZE DRYER COMPONENTS APPENDIX B FREEZE DRYER DIMENSIONS APPENDIX C FREEZE DRYER SPECIFICATIONS Electrical Specifications Environmental Conditions APPENDIX D FREEZE DRYER ACCESSORIES DECLARATION OF CONFORMITY 44 47 47 48 49 56 CHAPTER 1 INTRODUCTION Congratulations on your purchase of a Labconco FreeZone Freeze Dry System which is designed for laboratory lyophilization procedures The refrigerant used in the refrigeration system 1s CFC free so it wi
20. Requirements The freeze dryer should be located in an area that provides an unobstructed flow of air around the cabinet This air cools the refrigeration system The refrigeration system draws air through the rear panel and exhausts it through the sides A minimum of 3 must be allowed between the back and both sides of the freeze dryer and adjacent wall surfaces Restriction of airflow during operation could adversely affect performance 6 Product Service 816 333 8811 or 1 800 522 7658 Chapter 2 Prerequisites Refer to Appendix C Freeze Dryer Specifications for dimensional drawings of the Freeze Dryer The space for benchtop models must be large enough to accommodate the vacuum pump which will be used with the freeze dryer Vacuum Pump Requirements A vacuum pump must be provided by the user A vacuum pump with a displacement of 86 liters per minute and 0 002 mBar ultimate pressure is adequate for most samples The inlet fitting on the vacuum pump must be suitable for 3 4 ID vacuum hose which is provided It is recommended that the vacuum pump is equipped with an exhaust filter to minimize oil mist exhausting from the vacuum pump The operating vacuum level may be set on the freeze dryer The higher the pressure is set the more likely it is that oil mist will be exhausted Vacuum pumps used with 115V models should be equipped with a 115V 15 Amp NEMA 5 15P plug Vacuum pumps used with 230V models should be equipped with a reverse IEC
21. TO CLEAR IT Press and hold SELECT for five seconds until the error message is cleared from the display Line Voltage Out of Range If the voltage supplied to the freeze dryer varies beyond allowable limits the alarm will be activated When an alarm occurs press MENU and the display shows LINE VOLTAGE ERROR The alarm self cancels when the proper voltage is restored The high and low alarm points are preset at the factory to correspond to the normal allowable voltage variations based on the nominal voltage specified for the freeze dryer Some models may be operated outside the normal voltage limits See Appendix C If necessary the high and low alarm points may be adjusted for these models See Chapter 8 Product Service 816 333 8811 or 1 800 522 7658 23 Chapter 4 Using Your Freeze Dryer Temperature Out of Range An alarm activates if the collector temperature rises above 40 C 60 C for Models 73820xx If this occurs the display shows COLLECTOR TEMPERATURE HOLD SELECT TO CLEAR IT Press and hold SELECT for five seconds until the error message 1s cleared from the display Service Vacuum Pump The vacuum pump normally plugs into the vacuum pump electrical receptacle on the back of the freeze dryer When the freeze dryer has accumulated a total of 1000 operating hours the alarm indicator will flash Press MENU to display the alarm CHANGE VACUUM OIL HOLD SELECT TO CLEAR IT Press and hold SELECT for five seco
22. The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen 15 days of delivery Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various federal state or local regulations All users of this equipment are required to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Labconco Corporation is held harmless with respect to user s compliance with such regulations Contacting Labconco Corporation If you have questions that are not addressed in this manual or if you need technical assistance contact Labconco s Customer Service Department or Labconco s Product Service Department at 1 800 821 5525 or 1 816 333 8811 between the hours of 7 00 a m and 6 00 p m Central Standard Time Part 7387900 Rev B ECO G733 TABLE OF CONTENTS CHAPTER 1 INTRODUCTION Freeze Dry Process Freeze Dry Rates Freeze Dry Capacity Samples Containing Volatile Substances Safety Symbols CHAPTER 2 PREREQUISITES Electrical Requirements Location Requirements Vacuum Pump Requirements Chamber or Manifold Requirements CHAPTER 3 GETTING STARTED Unpacking Your Freeze Dryer Freeze Dryer Components Setting Up Your Freeze Dryer Vacuum Pump Connection Electrical Connection Drying Chamber or
23. ar and below the display will show the actual vacuum When the system vacuum is between 0 450 and 0 133 mBar the lower green vacuum graph LED will flash When the system vacuum level is lt 0 133 mBar the green LED will be lit steadily indicating that samples may be added Product Service 816 333 8811 or 1 800 522 7658 19 Chapter 4 Using Your Freeze Dryer Setting the Operating Vacuum Level The vacuum level may be set by the user to optimize the freeze dry process Normally the sublimation rate will increase if there is less vacuum a higher pressure in the freeze dryer A good starting place is to set the vacuum so its level is equivalent to about 10 C colder than the eutectic or collapse temperature of the sample Adjustments to the vacuum level must be made for various freeze drying conditions Factors that must be considered are whether the sample 1s freeze dried on heated shelves or in glassware attached to manifold valves the volatility of the sample itself the size of the sample and the heat energy supplied to the sample Some guidelines for setting the freeze dryer are shown below These show pre freezing temperatures and vacuum levels Exact protocols must be determined by the user for the specific samples that are being freeze dried When the vacuum control is set to operate at less vacuum the ice holding capacity of the collector may be decreased Solidification Eutectic Pre Freeze Vacuum Set Point Temperature Tempera
24. ate a text file or spreadsheet to tabulate and plot the data Consult your laboratory supply dealer regarding the latest software available The following describes how to use an IBM compatible computer with Hyper Terminal software included with Windows 95 98 XP or 2000 operating systems to collect the RS232 data from your freeze dryer 1 Make sure that the freeze dryer is properly connected to the communication port on the computer 2 Open Hyper Terminal software 1 Windows 95 or 98 use START PROGRAMS ACCESSORIES 11 Windows 2000 use START ROGRAMS ACCESSORIES COMMUNICATIONS The first time Hyper Terminal is opened a dialogue box requesting an area code and phone number appears Enter the appropriate numbers and continue 28 Product Service 816 333 8811 or 1 800 522 7658 Chapter 6 Using the RS232 Receptacle 3 The Connection Description dialogue box opens Type in a user defined name and select an icon for the new connection Press OK 4 The Connect To dialogue box opens Using the down arrow selection button select the communication port to which the cable has been connected Press OK Product Service 816 333 8811 or 1 800 522 7658 29 Chapter 6 Using the RS232 Receptacle 5 The Com X Properties dialogue box opens Enter the appropriate data properties and press OK 6 When the freeze dryer main power switch is on the data is transmitted and update
25. ce and the heat removed from the collector to convert the water vapor into ice Product Service 816 333 8811 or 1 800 522 7658 1 Chapter 1 Introduction Freeze Dry Rates The efficiency of the freeze drying process is dependent upon the surface area and the thickness of the sample the collector temperature and vacuum obtained the eutectic point and solute concentration of the sample It is important to remember these factors when trying to obtain efficient utilization of your freeze dry system A listing of selected materials and their approximate drying times are shown in Table 1 for your reference Table 1 SAFE TEMPERATURE AND DRYING TIMES FOR SELECTED MATERIALS Material Hours 10mm Thick Approx 4 40 24 48 Total sample quantities are contingent on various freeze dryer capacities Up to the point of overloading the system the greater the surface area of the sample the faster the rate of freeze drying By contrast for a given surface area the thicker the sample the slower the rate of freeze drying This is based on the fact that the heat of sublimation is usually absorbed on one side of the frozen sample and must travel through the frozen layer to vaporize water at the other surface In addition as the sample is freeze dried the water vapor must travel through the layer of dried material The thicker the sample the greater the chance that the dried layer may collapse which would cause an additional decrease in th
26. cleaned up NOTE The disposal of substances used in connection with this equipment may be governed by various Federal State or local regulations All users of this equipment are urged to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Product Service 816 333 8811 or 1 800 522 7658 13 CHAPTER 4 Usinc Your FREEZE DRYER After your Freeze Dryer has been installed as detailed in Chapter 3 Getting Started you are ready to begin using your Freeze Dryer Read this chapter to learn how to e operate the controls e understand the display e connect samples WARNING Do not use the freeze dryer in a manner not specified by the manufacturer refer to Appendix C Freeze Dryer Specifications The electrical protection properties of the freeze dryer may be impaired if the freeze dryer is used inappropriately 14 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Using Your Freeze Dryer Freeze Dryer Controls The control panel for the freeze dryer is shown below with a description about its function a O E D O mo TEMP 0 1 LCD Display Displays system operating parameters set up parameters and alarm messages 2 Menu Switch This switch is used to change the display from operating system parameters to set up parameters 3 Select Switch Used to selec
27. cuum pump power cord A plug is supplied that can be installed on the vacuum pump power cord if necessary Make certain that the oil in the vacuum pump is at the proper level Electrical Connection Plug the power cord into the receptacle on the back of the freeze dryer and plug the other end into a suitable power receptacle Drying Chamber or Drying Manifold Installation Install the clear lid with 3 0 inch hole on top of the collector chamber The supplied drying chamber or drying manifold which may be purchased separately may be positioned directly above the 3 inch hole in the collector chamber lid Center the rubber gasket that came with the chamber over the hole in the lid Then place the chamber over the gasket When vacuum is applied to the system the chamber will be held securely in place Product Service 816 333 8811 or 1 800 522 7658 11 Chapter 3 Getting Started Chemical Resistance of Freeze Dryer Components The FreeZone Freeze Dry System is designed to be chemically resistant to most compounds that are commonly used in freeze drying processes However by necessity the freeze dryer is comprised of a number of different materials some of which may be attacked and degraded by certain chemicals The degree of degradation is dependent on the concentration and exposure duration Some of the major components of the FreeZone Freeze Dry System that are susceptible to degradation are as follows Acids Buffers Solvents lt
28. d at the time intervals selected by the user LABCONCO HyperTerminal File Edit View Call Transfer Help Dle al 3 alal l B lt T 40V 18 SF 999C 999PG6 9 gt B lt T 40V 18 SF 999C 999PG6 9 gt B lt T 40V 18 B lt T 40V 18 B lt T 40V 18 SF 9990 999PG 9 gt B lt T 40V 18 SF 999C 999PG6 9 gt B lt T 40V 18 SF 9990 999PG 9 gt B lt T 40V 18 SF 999C 999PG6 9 gt B lt T 48V 18 SF 999C 999PG6 9 gt 30 Product Service 816 333 8811 or 1 800 522 7658 CHAPTER 7 TROUBLESHOOTING Refer to the following if your freeze dryer fails to operate properly If the suggested corrective actions do not solve your problem contact Labconco for additional assistance FreeZone Freeze Dry Systems that are clean dry and without samples attached should reach a vacuum of 0 133 mBar within 10 minutes and should achieve an ultimate vacuum of 0 033 mBar within 18 hours when the refrigeration is operating If the freeze dryer does not obtain a satisfactory vacuum perform the following maintenance tests I Vacuum Pump First make sure that the vacuum pump operates If it fails to operate check the electrical connections of the freeze dryer to the power source and then check the electrical connection of the vacuum pump to the freeze dryer If the vacuum pump has a power switch make sure that it is turned on If the vacuum is not adequate when the vacuum pump is operating proceed with the following steps 1 Check the oil and ensure i
29. d manifolds Serum Bottles Serum Bottles Stoppers and seals are supplied in packages of 100 Threaded Vials Stoppers and threaded vials with Screw Caps are supplied in packages of 200 Size Vials with Stoppers Screw Caps 5 ml 7762300 7762200 10 ml 7762600 7762200 Seal Crimper Secures tear away Aluminum Seals 7578000 Seal Crimper for 13 mm corkage Shipping weight 3 lbs 1 4 kg 7578100 Seal Crimper for 20 mm corkage Shipping weight 3 lbs 1 4 kg Size 20 mm 13 mm Split Aluminum _ Sleeve Type Corkage Corkage Stoppers Seals Stoppers 2 ml 7575010 7576010 7577010 3 ml 7575210 7576010 7577010 5 ml 7573010 7576210 7577110 757710 10 ml 7573210 7576210 7577110 7577510 20 ml 7573410 7576210 7577110 7571510 30 ml 7573610 7576210 7577110 7577510 50 ml 7573810 7576210 7577110 7577510 100 ml 7574010 7576210 7577110 7577510 125 ml 7574210 7576210 7577110 7577510 52 Product Service 816 333 8811 or 1 800 522 7658 Fast Freeze Flasks Fast Freeze Flasks are specially designed to be easier to handle faster to load and more convenient to use than other freeze dry glassware now in your laboratory Compatible with all major brands of laboratory freeze dry equipment Fast Freeze Flasks eliminate the risk of contamination from vacuum grease reduce spillage of valuable samples and require no washers gaskets or retainers How to select Fast Freeze Flasks for your Freeze Dry System Select fast Freeze Flas
30. down when the temperature reaches 10 0 10 20 30 40 C 10 20 30 40 50 60 for Models 73820xx When the collector temperature 1s 40 C 60 C for Models 73820xx or lower the green indicator will light 12 Alarm Indicator This red LED indicates that a system alarm has occurred Press the Menu Switch to display the alarm message on the LCD display 13 Main Power Switch Turns the freeze dryer on or off Not shown located on the left side of the cabinet Operation Checklist The following checklist should be followed prior to each use of your freeze dryer 1 Wipe the interior of the collector chamber with a soft cloth or paper towel to remove any accumulated moisture 2 Check the collector chamber drain hose to ensure that the hose 1s free of moisture and that the drain plug is securely installed The freeze dryer will not start 1f moisture 1s detected 3 Using a soft lint free cloth or paper towel wipe the collector chamber lid gasket to remove any dirt and contaminants that could cause a vacuum leak Vacuum grease is not required on the lid gasket to obtain a proper vacuum seal 4 Remove the drying chamber or accessory manifold from the connection port and using a soft lint free cloth or paper towel wipe the port gasket and sealing surfaces of the drying chamber manifold to remove any dirt and contaminants that could cause a vacuum leak Reinstall the drying chamber or manifold on the port Vacuum grea
31. e rate of freeze drying The surface area and thickness of the sample can usually be ignored when each sample contains only a few milliliters However for larger volumes the samples should be shell frozen to maximize the surface area and minimize the thickness of the sample The volume of the freeze dry flask should be two to three times the volume of the sample 2 Product Service 816 333 8811 or 1 800 522 7658 Chapter 1 Introduction In order for lyophilization to occur ice must be removed from the frozen sample via sublimation This is accomplished by the collector and the vacuum pump The collector which should be at least 15 to 20 C colder than the eutectic temperature melting temperature of the sample traps vapor as ice Since the vapor pressure at the collector is lower than that of the sample the flow of water vapor is from the sample to the collector Since this vapor diffusion process occurs very slowly under normal atmospheric conditions a good vacuum is essential to maintain an efficient rate In many applications the maintenance of a vacuum of 0 133 mBar or less 1s recommended The rate of freeze drying is directly proportional to the vapor pressure and the vapor pressure is dependent upon both eutectic temperature and solute concentration of the sample For example a solution of sodium chloride and water would freeze dry at a slower rate than pure water The eutectic temperature of a sodium chloride solution is about
32. ers with a 9 pin D sub male serial connector should use Connect Cable Labconco part number 7537800 to connect a computer to the freeze dryer The purpose of the RS232 interface is to send data to a data logging computer to monitor the state and activity of the freeze dryer This data is half duplex data The data properties are as follows Data Rate 2400 Baud 8 Bit word length 1 Start bit 1 Stop bit No parity is transmitted Standard ASCII character set ae e Product Service 816 333 8811 or 1 800 522 7658 27 Chapter 6 Using the RS232 Receptacle The time between data transmissions may be varied by the user to occur at 10 30 60 300 or 600 second intervals Press the MENU button until the RS232 screen appears on the display RS 232 TRANSMISSION RATE 10 SECONDS Press select until the desired time interval is shown The format of the transmitted message is as follows B lt T 48 V 18 SF 999 C 999 PG 9 gt Where B Base Unit T Collector Temperature 48 Collector Temperature in Celsius V Vacuum 18 Vacuum Level in Micro Bars SF Shell Freezer Temperature not available on 4 5L models 9 Feature not installed or out of range C Vacuum Chamber Temperature not available on 4 5L models PG Purge not available on 4 5L models There are several commercially available software packages which can read RS232 data and enter the data into a computer program such as a word processor to cre
33. eze dryer manually use at least two 2 persons and follow safe lifting guidelines If your freeze dryer was damaged in transit you must file a claim directly with the freight carrier The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen 15 days of delivery Labconco Corporation and its dealers are not responsible for shipping damage Do not return goods without the prior authorization of Labconco Unauthorized returns will not be accepted Do not discard the carton or packing material for your freeze dryer until you have checked all of the components and installed and tested the freeze dryer Freeze Dryer Components Locate the model of FreeZone Plus Cascade 4 5 Liter Freeze Dry System you received in the following table Verify that the components listed are present and undamaged Power Cords Catalog Description le ppg C ce oe 7386021 4 5L Benchtop Cascade Freeze Dryer 115 60 x x 7386030 4 5L Benchtop Cascade Freeze Dryer 230 50 x 7386031 4 5L Benchtop Cascade Freeze Dryer 230 50 x x 7386040 4 5L Benchtop Cascade Freeze Dryer 230 60 x zo 6 x x 7386060 4 5L Benchtop Cascade Freeze Dryer 230 60 x 7386061 4 5L Benchtop Cascade Freeze Dryer 230 50 x x 7386070 4 5L Benchtop Cascade Freeze Dyer 230 60 x x E 1336400 332701 P
34. gh x 7 7 8 diameter 304 stainless steel with 34 vacuum connections For use with dry ice and solvent Cools to approximately 75 C Product Heater 115V For use in chamber 7522800 Provides 3 heated shelves operating at 43 C 110 F Product Heater 230V For use in chamber 7522800 Provides 3 heated shelves operating at 43 C 110 F Clear Drying Chamber with 8 Valves For bulk drying or drying in flasks Drying Rack Unheated Provides three shelves For use in Chambers 7443500 and 7522800 Clear Drying Chamber For bulk drying 20 Port Manifold 10 high x 27 2 wide x 8 5 8 deep Type 304 stainless steel manifold with 20 freeze dry valves 48 Port Ampule Manifold 28 5 8 high x 5 wide x 5 deep Type 304 stainless steel with 48 each 1 4 tube stems Sample Valve Kit Includes neoprene valve body knob and installation parts 16 Port Drying Chamber 13 high x 138 diameter 304 stainless steel with 16 freeze dry valves Product Service 816 333 8811 or 1 800 522 7658 51 Appendix D Freeze Dryer Accessories Serum Bottles and Vials Perfect for long term storage of freeze dried samples Labconco Serum Bottles and Treaded Vials are specifically designed for lyophilization applications Their uniform thin wall construction ensures even freezing and drying Bottles and vials are ideal containers for use in the FreeZone Stoppering Tray Dryer Serum bottles also connect to valve ports on drying chambers an
35. he vacuum pump when there is liquid in the collector chamber This could result in damage to the vacuum pump 22 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Using Your Freeze Dryer Alarms A number of unusual events may occur during a lyophilization procedure that can adversely effect the operation of the freeze dryer If an event occurs the alarm indicator flashes and the beeper sounds The beeper automatically mutes itself after one minute The specific alarm can be identified by pressing MENU Pressing MENU multiple times displays multiple alarms 1f they have occurred The following out of specification conditions initiate an alarm Power Failure If a power failure occurs during a freeze dry process the vacuum control valve allows air to bleed into the freeze dryer system If the failure is of a short duration and the collector does not warm up excessively when power is restored the freeze dryer restarts and resume operation of the refrigeration and vacuum systems If the power failure lasts for a longer duration and the collector warms above safe limits when the power is restored the freeze dryer will not automatically restart This prevents melted sample from being drawn into the collector and prevents liquid from being sucked into the vacuum pump When power is restored the alarm indicator flashes and the alarm beeper sounds When the MENU button is pressed the display shows POWER FAIL HOLD SELECT
36. ht Flat Bottom 1 ml 7572710 7570610 2 ml 7570810 4 ml 5 ml 7571010 10 ml 7571210 25 ml 50 ml ti the Oxygen Natural Gas Sealing Torch 7578500 Torch specifically designed for flame sealing freeze dry ampules Seals all types of heat resistant glass Connects to natural gas butane or propane and oxygen with 1 4 ID hose connectors Shipping weight 3 Ibs 1 4 kg Ampule Valve Adapters 7593401 Adapter connects ampules to valve ports or stainless steel stems Ten per package Shipping weight 0 5 lbs 0 2 kg Trident Adapter 7762700 Three Way Adapter permits attachment of three ampules to a single valve port Ampules are attached using Ampule Valve Adapters or 1 8 surgical tubing not provided Cavity in adapter body can be filled with cotton fiber media to help prevent contamination between samples Shipping weight 3 oz 0 1 kg Product Service 816 333 8811 or 1 800 522 7658 55 Protecting your FA lanoratory environment as LABCONCO CORPORATION 816 333 8811 800 821 5525 8811 Prospect Avenue FAX 816 363 0130 Kansas City MO 64132 2696 www labconco com CE Declaration of Conformity We Labconco Corporation 8811 Prospect Avenue Kansas City MO 64132 USA Ph 816 333 8811 Fax 816 363 0130 labconco labconco com being the representative party in the EU solely responsible for this certification hereby declare that the following product Equipment Laboratory Equipment FreeZone
37. ks based on your sample sizes Flasks should be filled no more than one third of their volume so that maximum surface area is achieved and efficient lyophilization is assured A complete Fast Freeze Flask includes a rubber top glass bottom and a supply of filter paper Tops bottoms and filter paper are available separately as replacement components Adapters are required to attach flasks to freeze dry valve ports Appendix D Freeze Dryer Accessories Compatible with all major brands of laboratory freeze dry apparatus Variety of adapters available You may select from glass or stainless steel adapters available straight or with 45 bend Required order separately No complex filter retainer is necessary For those who use filters they are easily inserted between the adapter and top of the flask One hun dred filters are sup plied with each flask Top seals easily The flexible silicone rubber top snaps on and off easily yet provides a reliable high vacuum seal Sia The complete Fast Freeze Flask has only two components ea high strength Horizontal shell borosilicate freezing Flask glass bottom may be placed in to withstand a horizontal posi extreme tion and rotated temperatures in a bath for shell and high freezing vacuum a flexible non contaminating silicone rubber top No vacuum grease is necessary Wide mouth opening loads fast cleans easily Available
38. le allow system vacuum to return to 0 133 mBar or lower Any combination of valves and sample sizes may be utilized at one time provided that the system vacuum and collector temperature remain sufficiently low to prevent melting of the frozen sample 3 When all the frost has disappeared from the outer surface of the sample container and no cold spots can be detected by handling the container the sample is nearly dry To be certain of low final moisture content dry the sample for several hours past this point 4 To remove a container after drying is complete turn the plastic knob on the valve to the VENT position which closes the valve and vents the container Should backfilling with an inert gas be required connect the gas supply line to the vent port on the valve before turning the plastic knob on the valve to vent position The sample container may now be removed In the vent position the bevel on the valve knob should point away from the sample port Product Service 816 333 8811 or 1 800 522 7658 21 Chapter 4 Using Your Freeze Dryer 5 Ampules may be flame sealed while connected to a valve by using a sealing torch Care must be taken not to burn the valve An insulation material placed between the valve and the torch is recommended Shut Down At the end of a run or when a sufficient amount of condensate accumulates on the collector coil to obstruct the flow of vapor to the collector chamber the Freeze Dryer should be defr
39. ll not endanger the environment The unit is easy to install and maintain Proper care and maintenance of this product will result in many years of dependable Service Freeze Dry Process Freeze drying is an important process in sample preparation and for the preservation and storage of biologicals pharmaceuticals and foods Of the various methods of dehydration freeze drying lyophilization is especially suited for substances that are heat sensitive Other than food processing e g coffee whole dinners freeze drying has been extensively used in the development of pharmaceuticals e g antibiotics and preservation of biologicals e g proteins plasma viruses and cell lines The nondestructive nature of this process has been demonstrated by the retention of viability in freeze dried viruses and microorganisms Freeze drying 1s a process whereby water or other solvent is removed from frozen material by converting the frozen water directly into vapor without the intermediate formation of liquid water The basis for this sublimation process involves the absorption of heat by the frozen sample in order to vaporize the ice the use of a vacuum pump to enhance the removal of water vapor from the surface of the sample the transfer of water vapor to a collector and the removal of heat by the collector in order to condense the water vapor In essence the freeze dry process is a balance between the heat absorbed by the sample to vaporize the i
40. nded if the vacuum pump is operated for an extended period of time after a freeze dry run This allows contaminants to be purged from the hot oil This must be done with the inlet to the pump blocked off to prevent air from free flowing through the pump If the pump is operated at an elevated vacuum level gt 10mBar oil may be expelled from the pump and damage could occur Another way to extend the life of the vacuum pump is to install an optional secondary trap in the line between the freeze dryer and the vacuum pump Contact Labconco for ordering information With prudent maintenance the FreeZone Freeze Dry System will provide years of service Warranty on the affected parts will be voided if maintenance has been obviously neglected If you have questions about using specific compounds in the freeze dryer contact Labconco Technical Service at 1 800 821 5525 or 816 333 8811 or e mail labconco labconco com 4 amp CAUTION Solvent Safety Precautions Solvents used in the freeze dryer may be flammable or hazardous to your health Use extreme caution and keep sources of ignition away from the solvents When using flammable or hazardous solvents the vacuum pump must be vented to or operated inside a fume hood Hazardous materials such as strong acids or bases radioactive substances and volatile organics must be handled carefully and promptly cleaned up if spilled If a sample is spilled in the collector chamber it must immediately be
41. nds until the error message is cleared from the display This resets the 1000 hour timer It may be necessary to service the vacuum pump more frequently than every 1000 hours depending on the operation of the freeze dryer The pump oil should be regularly monitored to verify that it is clean Moisture in Collector If there is liquid in the collector chamber and either a REFRIGERATION button or the VACUUM button is pressed neither function will start and an alarm will be activated When MENU 1s pressed the display shows MOISTURE IN COLLECTOR When the collector is drained the alarm self cancels This feature prevents the inadvertent start of the vacuum pump when liquid is in the collector and thereby can extend the life of the vacuum pump A few drops of liquid may remain in the drain hose after draining When the vacuum pump 1s started these drops may be sucked into the collector chamber This is normal 24 Product Service 816 333 8811 or 1 800 522 7658 CHAPTER 5 MAINTAINING YOUR FREEZE DRYER Under normal operation the freeze dryer requires little maintenance The following maintenance schedule is recommended As needed 1 Clean up all spills remove liquids from the chamber Clean lid and gasket using soft cloth sponge or chamois and a mild non abrasive soap or detergent 3 Check oil level of the vacuum pump It should be between MIN and MAX If the oil level is less than an inch 25 4 mm above MIN add
42. oil to proper level 4 If oil shows cloudiness particles or discoloration drain the pump and replace with fresh oil 5 Utilization of acids requires immediate cleaning and neutralization after a run or physical damage to the collector chamber and collector coil will result 6 Check the collection chamber for condensed or frozen solvents and dispose of appropriately Completely empty the collector chamber before the next run or before attempting to start the vacuum pump 7 Ifsample valves leak or stems become difficult to turn pull the stem out of the rubber valve body and apply a thin coat of vacuum grease to the stem Reinsert the stem into the valve body Product Service 816 333 8811 or 1 800 522 7658 25 Chapter 5 Maintaining Your Freeze Dryer Monthly 1 The rubber components on the freeze dryer may eventually deteriorate and require replacement The effective life of rubber parts depends upon both their usage and the surrounding environment Check all rubber hoses and gaskets and replace any that show signs of hardening permanent set or deterioration 2 Using a soft cloth sponge or chamois and a mild non abrasive soap or detergent clean the acrylic chamber lid 3 Using a soft cloth sponge or chamois and a mild non abrasive soap or detergent clean the exterior surfaces of the freeze dryer Liquid spray cleaners and polishes may be used on the exterior surfaces Do not use solvents to remove stains from the exterior
43. op to attach flasks to freeze dry valve ports 3 4 valve 7568400 Connects 24 40 STj Flask Top to 1 2 valve 7568600 Connects 24 40 STj Flask Top to 3 4 valve Complete Complete Dimensions Flask Size Flask Flask Bis Si Poe a sea ers Flask Bottom 19 38 STj 24 140 STj Hx ID 25 ml 7550000 7550000 7552000 7556000 7559000 7557000 37mm x 34mm 50 ml 7550200 7554200 7552000 7556000 7559000 7557200 67mm x 34mm 100 ml 7550400 7554400 7552200 7556200 7559200 7557400 50mm x 59 2mm 250 ml 7550600 7554600 7552200 7556200 7559200 7557600 110mm x 59 2mm 500 ml 7550800 7554800 7552400 7556400 7559400 7557800 103mm x 90 2mm 750 ml 7550900 7554900 7552400 7556400 7559400 7557900 145mm x 90 2mm 1000 ml 7551000 7555000 7552400 7556400 7559400 7558000 187mm x 90 2mm Valves on the freeze dry system drying chambers and manifolds offered in this catalog fit adapters of both 1 2 and 3 4 sizes Size information is provided for selecting adapters for older Labconco models and other freeze dryers 54 Product Service 816 333 8811 or 1 800 522 7658 Appendix D Freeze Dryer Accessories Ampules Labconco Ampules are fabricated of highest quality borosilicate glass for strength and durability They are available in five different configurations to provide the ideal ampule for your specific lyophilization requirements from tray drying to flame sealing applications Ampules Ampules are supplied in packages of 100 Pre Scored Size Straig
44. osted First release system vacuum by turning the plastic knob on a valve to the open position or by pulling the collector chamber drain plug out of the drain hose Now press the Vacuum Switch on the control panel to turn the vacuum pump OFF Press the Refrigeration Switch next to the illuminated LED to turn OFF the refrigeration system Turn OFF the Main Power Switch on the right hand side of the cabinet Defrosting The following procedure should be followed when defrosting the collector coil 1 Pull the collector chamber drain hose out from the right hand side of the freeze dryer and remove the drain plug Place the drain hose in a suitable container to collect the condensate that will melt off the collector coil 2 Remove the collector chamber lid and allow ambient room air to melt the ice Dispose of the liquid appropriately Flush the collector chamber with water and wipe chamber dry 4 Ifrapid defrost is desired pour warm water over the collector coil Do not allow the liquid to enter the vacuum port on the upper rear of the chamber 5 Reinstall the drain hose plug and slide drain hose back into the side of the cabinet Dispose of the liquid appropriately U Utilization of acid requires immediate cleaning and neutralization after defrost or physical damage to the collector chamber and collector coil will result Do not attempt to chip ice from the collector coil as damage may occur to the coil Never attempt to start t
45. plug This plug is included with 230V models This will allow the vacuum pump to be plugged into the receptacle on the back panel of the freeze dryer See Appendix D Freeze Dryer Accessories for vacuum pumps available from Labconco Chamber or Manifold Requirements A 12 port drying chamber is included with the unit This allows samples to be attached to the freeze dryer See Appendix D Freeze Dryer Accessories for available chambers and manifolds Product Service 816 333 8811 or 1 800 522 7658 7 CHAPTER 3 GETTING STARTED Now that the site for your freeze dryer is properly prepared you are ready to unpack inspect install and test your freeze dryer Read this chapter to learn how to unpack and move your freeze dryer set up your freeze dryer connect the electrical supply source to your freeze dryer properly exhaust your freeze dryer safely use solvents with your freeze dryer 8 Product Service 816 333 8811 or 1 800 522 7658 Chapter 3 Getting Started Unpacking Your Freeze Dryer Carefully unpack your freeze dryer and inspect it for damage that may have occurred in transit If your freeze dryer is damaged notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier The Benchtop FreeZone Plus Cascade 4 5 Liter Freeze Dry System weighs over 125 lbs 57 Kg The Console FreeZone Plus Cascade 4 5 Liter Freeze Dry System weighs over 176 lbs 80 Kg If you must lift the fre
46. r or moderate injury CAUTION used without the safety alert C AUTI ON symbol indicates a potentially hazardous situation which if not avoided may result in property damage It is important that you understand the warnings listed throughout this manual before you operate the freeze dry system Product Service 816 333 8811 or 1 800 522 7658 5 CHAPTER 2 PREREQUISITES Before you install your freeze dryer you need to prepare your site for installation Carefully examine the location where you intend to install your freeze dryer You must be certain that the area 1s level and of solid construction An electrical source must be located near the installation site Carefully read this chapter to learn e the electrical supply requirements e the vacuum pump requirements Refer to Appendix C Freeze Dryer Specifications for complete freeze dryer electrical and environmental conditions specifications and requirements Electrical Requirements The freeze dryer requires a dedicated electrical outlet This outlet requires a 20 Amp circuit breaker or fuse for models rated at 115V 60 Hz An outlet equipped with a 15 Amp circuit breaker or fuse is required for models rated at 230V 50 60 Hz See Freeze Dryer Components in Chapter 3 for detail plug specifications If the plug supplied with the Freeze Dryer does not match the available receptacle remove this plug and replace it with an approved plug of the suitable style Location
47. roduct Service 816 333 8811 or 1 800 522 7658 9 Chapter 3 Getting Started Catalog Description i 7387020 4 5L Console Cascade Freeze Dryer 115 6o x _ 7387021 4 5L Console Cascade Freeze Dryer 115 60 x x 7387030 4 5L Console Cascade Freeze Dryer 230 50 Ix 7387031 4 5L Console Cascade Freeze Dryer 230 50 x x 7387040 4 5L Console Cascade Freeze Dryer 230 60 X 7387041 4 5L Console Cascade Freeze Dryer 230 0 x x 7387060 4 5L Console Cascade Freeze Dryer 230 50 Ixl 7387061 4 5L Console Cascade Freeze Dryer 230 50 x X 7387070 4 5L Console Cascade Freeze Dryer 230 50 x 7387071 45L Console Cascade Freeze Dryer 230 50 x x 4 5L 105C Benchtop Freeze Dryer 115 6o x fof fof 7382021 45L 105C Benchtop Freeze Dryer 115 60 x x 7382030 45L 105C Benchtop Freeze Dryer 230 50 x 7382031 4 5L 105C Benchtop Freeze Dryer 230 50 x x 7382032 4 5L 105C Benchtop Freeze Dryer 230 50 _ XJ 7382033 4 5L 105C Benchtop Freeze Dryer 230 50 x X 7382034 4 5L 105C Benchtop Freeze Dryer 230 50 X 7382035 4 5L 105C Benchtop Freeze Dryer _ 230 50 x 1x 7382040 4 5L 105C Benchtop Freeze Dryer 230 60 x 7382041 4 5L 105C Benchtop Freeze Dyer 230 60
48. rom vacuum pump exhaust Fits Vacuum Pumps 1472100 and 7739402 1473200 Replacement Element Oil Mist Pump Exhaust Filter Fits Pump Exhaust Filter 1473400 1473300 Replacement Element Odor Pump Exhaust Filter package of 5 Fits Pump Exhaust Filter 1473400 or Vacuum Pumps 1472100 and 7739402 1988000 Vacuum Pump Oil 1 Liter A molecularly distilled hydrocarbon oil with low vapor pressure For Vacuum Pumps 1472100 and 7739402 7772000 Soda Acid Trap Secondary trap that prevents migration of corrosive chemicals into vacuum pump 7772100 Replacement Media for Soda Acid Trap 7772700 Vacuum Pump Oil 1L A mineral oil with low vapor pressure For Vacuum Pumps 7438700 7438800 7769600 7769800 7670400 Replacement Filter Element Disposable filter that removes oil mist from the vacuum pump exhaust Fits Vacuum Pumps 7438700 7438800 7469600 and 7469800 7584300 Vacuum Pump Oil For Hybrid vacuum pumps 7584000 7584002 7772500 Carbon Solvent Trap Secondary trap that prevents migration of organic solvents into vacuum pump 50 Product Service 816 333 8811 or 1 800 522 7658 Appendix D Freeze Dryer Accessories PART DESCRIPTION 7772600 7538000 7509200 7509201 7443500 7442100 7442900 7522300 7868500 7509600 7522900 Replacement Activated Carbon Media for Carbon Solvent Trap Secondary Vacuum Cold Trap Provides additional protection for the vacuum pump when processing low eutectic samples 9 34 hi
49. rom date of shipment from Labconco whichever is sooner SteamScrubber amp FlaskScrubber Glassware Washers carry a two year warranty from date of installation or three years from date of shipment from Labconco whichever is sooner Blood Drawing Chairs carry a ten year warranty Carts carry a lifetime warranty Glassware is not warranted from breakage when dropped or mishandled This limited warranty covers parts and labor but not transportation and insurance charges In the event of a warranty claim contact Labconco Corporation or the dealer who sold you the product If the cause is determined to be a manufacturing fault the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation Under no circumstances shall Labconco Corporation be liable for indirect consequential or special damages of any kind This statement may be altered by a specific published amendment No individual has authorization to alter the provisions of this warranty policy or its amendments Lamps and filters are not covered by this warranty Damage due to corrosion or accidental breakage is not covered Returned or Damaged Goods Do not return goods without the prior authorization from Labconco Unauthorized returns will not be accepted If your shipment was damaged in transit you must file a claim directly with the freight carrier Labconco Corporation and its dealers are not responsible for shipping damages
50. se is not required on the port gasket to obtain a proper vacuum seal 5 Check that each sample valve is closed or in the vent position Vacuum Pump Ballast Setting Most vacuum pumps are equipped with a gas ballast mechanism The freeze dry process requires high vacuum Therefore it is recommended that the gas ballast be closed during the operation of the freeze dry system If the gas ballast is left open for extended periods of operation the oil can be pumped out the exhaust causing the pump to fail 16 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Using Your Freeze Dryer Operating the Freeze Dryer Set Up The freeze dryer may be configured to automatically start the vacuum pump when the collector temperature reaches 40 C The display units for vacuum may be selected to be mBar Pascal Pa or Torr and the temperature may be displayed as F or C The run time of the refrigeration system and the vacuum pump may be monitored To configure your freeze dryer turn the main power switch ON and press MENU The display shows VACUUM UNITS mBar Pa e Press SELECT until the desired units are flashing e Press MENU e The display shows VACUUM SET POINT XXXX XXY Where YYY is the units selected above e Press SELECT until the desired vacuum operating level is displayed See Setting the Operating Vacuum Level for guidelines e Jf 0 is selected the vacuum control is disabled and the vac
51. surfaces as they may damage the finish Annually 1 Every 12 months or more often if the freeze dryer is operated in a dusty environment the refrigeration system condenser should be cleaned Using a vacuum cleaner with brush attachment clean the condenser to ensure proper airflow for peak performance Front Access Panel Removal Console Models The front stainless steel panel can be removed by placing a flat blade screwdriver in the rectangular hole in the center of the kick panel Pivot the handle downward to disengage the latch and simultaneously pull the bottom edge of the lower panel forward The lower panel is installed by first sliding the top edge under the lip on the plastic control panel Pivot the bottom inward and push until the latch is secure 26 Product Service 816 333 8811 or 1 800 522 7658 CHAPTER 6 Usinc THE RS232 RECEPTACLE The operation of the freeze dryer can be monitored using a computer when it is connected to the RS232 receptacle on the rear panel The computer cannot control the operation of the freeze dryer The monitored parameters are the collector temperature in C and vacuum in microbars Computer Connection for Computer Interface Check your computer to see which type of serial port 1s provided then use a connecting cable below 1 Computers with a 25 pin D sub male serial connector should use Connect Cable Labconco part number 7537801 to connect a computer to the freeze dryer 2 Comput
52. t a rubber stopper in the small hole in the rear of the collector chamber and another in the drain port in the bottom 2 Start the freeze dryer and observe the vacuum indication If the vacuum is good the problem is in the collection chamber If it is bad the problem is in the plumbing connections from the collection chamber to the vacuum pump 3 Ifthe leak is in the chamber check the bulkhead fittings where the collection coil passes through the button of the chamber 4 Ifthe leak is in the plumbing connections check all rubber parts the drain hose the drain plug fittings and clamps STOPPER AWDLBBRALRBRBVAS QUEER WA Runnin ER Checking the connections to the pump and the vacuum tube Product Service 816 333 8811 or 1 800 522 7658 35 Chapter 7 Troubleshooting Refrigeration Module Operation Under a no load condition FreeZone Plus Cascade Freeze Dry Systems should achieve a collector temperature of 84 C models 73860xx and 73870xx or 105 C models 73820xx or lower when the vacuum pump is operating If the collector temperature does not reach the design temperature within 30 minutes then the refrigeration module is not functioning properly Cascade Systems consist of two vapor compression refrigeration systems in series connected together via a heat exchanger Each system has a compressor condenser capillary tube metering device and an evaporator During the operation the evaporator of the
53. t is clear and clean If the oil looks cloudy or has any particulates replace the pump oil Sometimes it may be necessary to flush the pump with clean oil several times To flush the pump run the pump 5 to 10 minutes to allow the oil to warm up Drain the oil and refill with clean oil Repeat as necessary 2 Check the oil level in the pump Ensure it is filled to the correct level 3 Check vacuum hose connections from the pump to the freeze dryer and try running the unit Product Service 816 333 8811 or 1 800 522 7658 31 Chapter 7 Troubleshooting If vacuum problems continue consider obtaining a second vacuum gauge capable of reading a vacuum of 0 010 mBar It is often useful in determining if the vacuum pump is operating properly and the vacuum sensor reading 1s accurate 4 Isolate the pump by disconnecting the vacuum hose from the freeze dryer Deadhead the pump by inserting the vacuum sensor from a secondary vacuum gauge into the end of the vacuum hose and observe the vacuum reading obtained Confirm that the pump is capable of achieving an ultimate vacuum less than 0 010 mBar or approximately 10 microns If an inadequate vacuum reading is obtained the pump has most likely failed and may need to be replaced or rebuilt ll Gaskets Tubing Connections Sample Valves l N 32 Check all sample valves on the drying chamber manifold and ensure all valves are closed or in the vent position Check to ensure all fittings are
54. t set up parameters 4 Vacuum Switch Used to start or stop the vacuum pump when operating in manual start up mode 5 Vacuum Indicator This green LED indicates that power is being supplied to the vacuum pump receptacle on the back of the freeze dryer 6 Manual Refrigeration Switch Used to start only the refrigeration module 7 Manual Operation Indicator When lit the green LED indicates the freeze dryer is being controlled manually by the operator Each function must be started by the operator 8 Auto Mode Switch Used to start or stop the refrigeration and the Auto Mode process 9 Auto Mode Indicator When lit the green LED indicates that the freeze dryer is in Auto Mode In this mode the vacuum pump will start when the collector temperature reaches 40 C 10 Vacuum Graph Display This display indicates the relative system vacuum level The highest LED indicates that the vacuum level is above 2 0 mBar The indicators will sequence down when the vacuum level reaches 2 0 1 0 0 8 0 6 0 45 0 12 mBar The lower green LED flashes when the system vacuum level is 0 45 to 0 12 mBar and illuminates steadily below 0 133 mBar Product Service 816 333 8811 or 1 800 522 7658 15 Chapter 4 Using Your Freeze Dryer 11 Collector Temperature Graph Display This display indicates the temperature of the collector The highest LED indicates the collector temperature 1s warmer than 10 C The indicators will sequence
55. ther 19 38 or 24 40 brands of labo standard taper joints ratory freeze have 90 bend dry apparatus Required order separately per flask no or ng Silicone rubber Ine compete grasps ee Flask am ak ag includes ible ri The flexible rin a flask top of holds securely i high e together during rosilicate pre freezing glass and lyophiliza a flexible non contaminating silicone rubber seal a flask bottom Lyph Lock Flasks simplify your obec Lyophilization procedures because they apc have only three pieces per flask a high and high strength borosilicate glass top and iii bottom with a silicone rubber ring seal Available The unique wide mouth design helps you A ai load samples easily and lyophilize ee efficiently be autoclaved How to select Lyph Lock Flasks for Akaki your Freeze Dry System Paaa Select Lyph Lock Flasks fast based on your sample sizes Flasks should be filled no more than one third of their volume so that maximum surface area is achieved and efficient lyophilization is assured A complete Lyph Lock Flask includes a glass top and bottom and a rubber ring seal Tops bottoms and seals are Adapters Add the adapters for connecting the Lyph Lock Flasks to the valve ports on your drying chamber or manifold 90 Bend Description Adapter available separately as replacement E e a SID eae F valve components Adapters are required to sscgoq Connects 19 38 STj Flask T
56. to occur Hence samples that contain volatile substances will have a greater tendency to melt particularly when placed in flasks or exposed to room temperature If a sample containing a volatile substance tends to melt when placed on a freeze dry system dilution of the sample with more water will help keep the sample frozen For example a 0 2M solution of acetic acid is much easier to freeze dry than a 0 5M solution Safety Symbols Your freeze dry system was designed with safety in mind however conditions may exist that could be hazardous a WARNING Read and understand operator s manual before using this machine Failure to follow operating instructions could result in death or serious injury 4 Product Service 816 333 8811 or 1 800 522 7658 Chapter 1 Introduction Throughout this manual potentially hazardous conditions are identified using the following words and symbols This is the safety alert symbol It is used to alert you to potential personal injury hazards Obey all safety messages that follow this symbol to avoid possible injury or death DANGER indicates an imminently DAN G F R azardous situation which if not avoided will result in death or serious injury WARNING indicates a potentially A WARNING hazardous situation which if not avoided could result in death or serious injury CAUTION indicates a potentially A CAUTION hazardous situation which if not avoided may result in mino
57. tures Bacteria Virus 40 C and Colder 50 C amp 0 040 mBar amp Colder Lower 15 to 23 1 65 to 0 77 Pre Freezing Samples Appropriate containers for freeze drying include ampules serum bottles and wide mouth freeze drying flasks Shell freezing of samples is recommended for wide mouth freeze drying flasks Smaller samples in ampules and serum bottles may be frozen in a freezer or in the center of the collector chamber The sample container size should always be at least two to three times the sample size 1 e 40 ml samples should be prepared in 80 ml containers or larger The temperature required for pre freezing is dependent on the characteristics of the sample Pre freezing temperature typically is at least 10 to 20 C below the eutectic or collapse temperature of the sample 20 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Using Your Freeze Dryer Adding Sample The following procedure should be followed when using sample valves in the freeze dry process 1 Connect a pre frozen sample to a sample valve on the drying chamber or manifold using an adapter Turn the plastic valve knob to the VACUUM position to open the valve which connects the attached sample to system vacuum The bevel on the knob should be positioned toward the sample port to apply vacuum to the sample Bevel in vent position Vent Backfill Port Bevel in vacuum position Adapter 2 Before adding another samp
58. uum level in the freeze dry system will be determined by the capabilities of the vacuum pump e Press MENU e The display shows TEMPERATURE UNITS C SF Product Service 816 333 8811 or 1 800 522 7658 17 Chapter 4 Using Your Freeze Dryer e Press SELECT until the desired units are flashing e Press MENU e The display shows REFRIG TOTAL HOUR XXXX SERVICE HOUR XXXX e To reset the SERVICE HOUR to 0 press SELECT This allows you to keep track of the time the refrigeration system operated since it was serviced e The display shows the refrigeration system hours REFRIG TOTAL HOUR XXXX RESET SERVICE HOUR e To reset the service hours to 0 continue to hold SELECT 5 seconds until hours are reset e TOTAL HOUR cannot be reset e Press SELECT e The display shows the vacuum pump hours VACUUM TOTAL HOUR XXXX SERVICE HOUR XXXX e To reset the SERVICE HOUR to 0 press SELECT e The display shows VACUUM TOTAL HOUR XXXX RESET SERVICE HOUR If you want to reset the service hours to 0 continue to hold SELECT five seconds until hours are reset e Press SELECT e The display shows the RS 232 data transmission rate RS 232 TRANSMISSION RATE 10 SECONDS 18 Product Service 816 333 8811 or 1 800 522 7658 Chapter 4 Using Your Freeze Dryer e To change the rate press and hold SELECT until the desired time interval is shown The time between data transmissions may be set to occur at 10
59. void over tightening Replace the valve body and knob and stem assembly Product Service 816 333 8811 or 1 800 522 7658 Chapter 7 Troubleshooting The illustrations below show how the sample valve installs on a chamber and a manifold Knob amp Stem Assembly Valve Body Valve Assembly Clear Chamber with Valves Manifold Assembly Valve Body Bulkhead Nut Knob amp Stem Thrust Washer Gasket Bulkhead Knob amp Stem a Valve Body Trim Ring Assembly No grease Fitting Assembly required Valve Assembly Manifold Valve Assembly Stainless Steel Chamber Product Service 816 333 8811 or 1 800 522 7658 33 Chapter 7 Troubleshooting lil System Components and Collection Chamber Isolation This test determines if the source of a leak is in the drying chamber 1 Remove the drying chamber or manifold 2 Leave the gasket on and turn a large freeze dry flask upside down to cover the connection port 3 Start the freeze dryer and observe the vacuum indication If the vacuum indication is good then the problem is in the drying chamber or manifold and you should return to Section IT Gaskets Tubing Connections Sample Valves FREEZE DRY FLASK GASKET gP a A A al a A P 7 A a E Isolating the freeze dryer from the accessory chamber manifold 34 Product Service 816 333 8811 or 1 800 522 7658 Chapter 7 Troubleshooting The following test determines if a vacuum leak is in the collection chamber 1 Inser

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