80 Orville Drive, Suite 102, Bohemia, NY 11716 U.S.A. (631) 567
Contents
1. Holder snaps into place The Disruptor Genie is now ready for use with the new Tube Holder If this equipment is used in a manner not specified by the manufacturer the pro tection provided by the equipment may be impaired CAUTION Unplug from power before cleaning Do not immerse Do not position the equipment so that it is difficult to disconnect the power cord SPECIFICATIONS The Disruptor Genie is classified as Installation Category 2 Overvoltage Category 2 and Pollution Degree 2 Environmental 0 C 38 C 32 F 100 F 95 Humidity max Indoor use only in elevation up to 2000m of altitude MODEL SI D238 SI D236 SI D237 SI D248 SI D246 SI D247 SI D288 SI D286 SI D287 POWER REQUIRED HZ AMPS 120V 230V 100V PARTS ASSEMBLY LIST To order parts for the DISRUPTOR GENIE 60 0 65 50 50 1 0 ai MADE IN THE cigs BS gt ETL Listed Conforms to UL Std 3101 1 Certified to CANIDSA C22 2 Contact your local distributor or visit www scientificindustries com Please specify Part No quantity and electric voltage Indicator No 2 3 4 5A 5B 5C 10 9 A G 8 Part No Description SPP0001 Spring OA D236 010 Mounting Bracket Assembly OK 0236 904 566 0028 00 0A D236 021 0A D246 021 0A D286 021 EPP0008 O0K 0236 408 OM D236 400 Eccentric with Clamp Assembly Spring Motor 120V Assembly Motor 230V Assembly Motor 100V Assembly Timer On Off Bottom C2. DISRUPTOR GENIE Cell Disruptor OPERATING INSTRUCTIONS Models SI D238 through SI D298 Models SI D236 through SI D296 1 5ml tubes only Models SI D237 through SI D297 2 0ml tubes only 80 Orville Drive Suite 102 Bohemia NY 11716 U S A 631 567 4700 Fax 631 567 5896 Toll Free 888 850 6208 Customer Service custsvc scientificindustries com www scientificindustries com U S PAT 4 781 487 The Scientific Industries Disruptor Genie is a vigorous mechanical cell disruption device Applications include cell disruption of yeast bacteria plant and animal tissue and for cell re suspension for DNA mini preps The Disruptor Genie may be used in a cold room Cell Disruption Using Glass or Zirconia Silica Beads The Disruptor Genie consists of a Vortex Genie Mixer base with a unique and patented accessory attached directly to the shaft eccentric of the mixer The Disruptor Genie dramatically increases the disruption efficiency of the disruption beads glass or zirconia silica because of the unique and patented design of the sample holder Models SI D238 through SI D298 holds twelve 1 5ml or 2 0ml microtubes Models SID236 through SI D296 holds twelve 1 5ml snap top microtubes and models SI D237 through SI D297 holds twelve 2 0ml screw cap microtubes Twelve sample tubes are placed in the Upper Ring of the attachment and allowed to project through corresponding holes in the Floating Ring below The holes in the Floating R
3. ing are smaller than the orbit path of the shaft Therefore as each of the twelve tubes travel in an orbit corresponding in size with the orbit path of the shaft the bottoms of the tubes repeatedly and vigorously collide within the confines of the holes in the Floating Ring This action in combination with the action of the disrup tion beads used inside the sample tubes results in a significant increase in the impact frequency and force The disruption efficiency is increased dramatically The Disruptor Genie is designed to run at full soeed It has a timer that may be set for the required disruption time or a switch position that allows for continuous operation until manually stopped Disruption Bead Selection Selection of bead size and material is important Use either glass or zirconia silica beads The following guide is for reference only It is strongly suggested that you use this guide only as a starting point to determine the optimum conditions for the particu lar cell disruption operation that you wish to perform The reference to Typical Run Time should result in a yield of 85 90 release of the cellular material for each of the conditions noted Normally 1 part of glass or zirconia silica beads to 2 parts of total liquid bio mass is the minimum condition recommended for effective cell disruption You can use more beads i e 4 parts of beads to 3 parts of bio mass providing that adequate agitation of the slurry is possible Generall
4. losure with Feet Kit Knob Indicator No 9A 9B 9C 9D 9E OF 9G 10 Part No 318 0510 02 OK 0246 901 OK 0256 901 OK 0266 901 OK 0276 901 OK 0286 901 ECP0021 SI 0565 0A 0563 010 0A 0563 011 Description 120V Line Cord 230V Line Cord No Plug 230V Line Cord European Plug 230V Line Cord British Plug 230V Line Cord Swiss Plug 100V Line Cord Australian Plug only Universal Microtube Holder Tube Holder Assembly 1 5ml Snap top tubes Tube Holder Assembly 2 0ml Screw top tubes Vortex Genie is a registered trademark of Scientific Industries Inc Scientific Industries Inc 2015 LTP0021 Rev D
5. otubes in the tube holder Space them evenly if using less than twelve tubes 4 Close the cover making sure that the stem of the tube holder goes into the cap in thecover 5 The Disruptor Genie is now ready for use Turning the timer knob clock wise starts the mixer It will run for up to 15 minutes depending on how far you turn the dial Turn the dial back counter clockwise to stop the unit before the time has expired otherwise the Disruptor Genie will turn itself off after the amount of time set Alternatively turning the timer knob firmly counter clock wise from the off position until a click is heard will turn on the Disruptor Genie in untimed mode Firmly turn the timer knob clock wise back to the off position to stop the unit At room temperature do not operate the mixer for more than three 3 hours continuously 6 Accessory Tube Holders may be ordered separately and used on the Disruptor Genie Part No SI 0565 Universal Microtube Holder Part No OA 0563 010 1 5ml Snap top Tube Holder and Part No 0A 0563 011 2 0ml Screw top Tube Holder To remove and replace the Tube Holder first make sure the Disrup tor Genie is turned off Pop off the Tube Holder by inserting your fingers under the top disk and lift the Tube Holder with a prying motion Align the flat surface of the Disruptor Genie mixing shaft with the flat surface of the shaft receptacle on the under side of the new Tube Holder Press downward until the new Tube
6. y the more beads that are used the faster the disruption of cells Type of Cell Bead Size Bead Material Typical Run Time Bacteria Spores 0 1 mm Borosilicate glass 2 to 3 min full speed Zirconia silica 1 to 2 min full speed Yeast Mycelia Microalgae 0 5 mm Borosilicate glass 3 to 4 min full speed Trypsinized cultures Zirconia silica 1 5 to 2 min full speed Cyanobacteria Sample Temperature During cell disruption friction inside the sample tubes will generate heat The Disruptor Genie is capable of being operated in a cold room or an environmental chamber In most short runs 3 minutes or less it is feasible that if you refrigerate the sample tubes beads and sample before the disruption run you will be able to perform the disruption at room temperature The small size of the microtubes may allow sufficient heat to dissipate so that sample integrity is not impaired The operator must make the decision based on the type of beads the sample content and size and the length of time of the run In any event it is advisable to keep the tubes beads and sample chilled before each run OPERATING INSTRUCTIONS We recommend that you retain your original packaging for 90 days in case you need to return the product for any reason to your distributor or Scientific Industries 1 Place the Disruptor Genie on a sturdy level work surface 2 Plug the line cord to a properly grounded three pronged electrical outlet 3 Load up to twelve micr
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