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Agilent 1120 Compact LC User Manual

1. 1120 Compact LC User Manual 4 Preventive Maintenance and Repair Autosampler 7 Use Needle Up to lift the needle to a position 8 Ensure the needle is aligned with the seat approximately 2mm above the seat Next Steps 9 On completion of this procedure Install the front cover 10 Select End in the Tools function Change Needle 100 1120 Compact LC User Manual Needle Seat Assembly When When the seat is visibly damaged When the seat capillary is blocked Tools required Flat head screwdriver Part number 1 G1313 87101 Parts required Description Preparations Remove the upper front cover Preventive Maintenance and Repair 4 1 4 inch wrench supplied in accessory kit Needle seat assy 0 17 mm i d 2 3 pl Select Start in the Tools function in LMD Software Change Needle Use the Needle Up command in the Change Needle function to lift the needle an addition 1 cm 1 Disconnect the seat capillary fitting from the injection valve port 5 L 2 Usea small flat head screwdriver to ease out the needle seat y 1120 Compact LC User Manual 101 4 Preventive Maintenance and Repair 3 Insert the new needle seat assembly Press 4 Connect the seat capillary fitting to port 5 of the seat firmly into position the injection valve ua ge l DN RU oy gt i DOF a
2. A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1120 Compact LC User Manual Contents General Information about the Agilent 1120 Compact LC System System Configurations 6 Site Requirements 7 Physical Specification 10 Performance Specifications 11 Installing the Agilent 1120 CompactLC 15 Unpacking your System 16 LAN Configuration 20 Introduction and Optimizing Performance 35 Overview of the Compact LC system 36 Solvent Delivery Systems 39 Injection System 47 Column Oven 60 Detector 61 Preventive Maintenance and Repair 65 Preventive Maintenance Scope of Work and Checklist 67 Early Maintenance Feedback EMF 68 Solvent Delivery System 70 Manual Injector 90 Autosampler 95 Detector 116 Parts for Maintenance and Repair 129 Parts for Compact LC System 130 Parts for Solvent Delivery System Maintenance 131 Parts for Injection System Maintenance 136 Column Oven 140 Parts for Detector Maintenance 141 Parts for Repair 143 1120 Compact LC User Manual Contents 5 Contents Appendix 151 General Safety Information 152 Solvent Information 155 Radio Interference 157 UV Radiation 158 Sound Emission 159 Declaration of Conformity for HOX2 Filte
3. test 3 Your pump must be connected to a control software e g EZChrom LMD OL etc with which the pressure and ripple can be monitored 4 The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition Table 16 Solvent Compressibility Solvent pure Compressibility 10 bar Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol lsopropanol Methanol 1 Propanol Toluene Water 126 115 95 110 100 118 114 104 120 150 100 100 120 100 87 46 1120 Compact LC User Manual Introduction and Optimizing Performance 3 How does variable stroke volume work Due to the compression of the pump chamber volume each plunger stroke of the pump will generate a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation is mainly dependent on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes will generate pressure pulsations of smaller amplitude than higher stroke volumes at same flow rates In addition the frequency of the pressure pulsations will be higher This will decrease the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes resulting in less flow ripple will improve composition ripple The pump uses a processor co
4. 3 50 Introduction and Optimizing Performance Injection System When the sample sequence begins the valve unit switches to the bypass position Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 i t Figure 18 Bypass Position Next the needle is raised and the vial is positioned below the needle The needle moves down into the vial and the metering unit draws the sample into the sample loop i Figure 19 Drawing the Sample 1120 Compact LC User Manual Introduction and Optimizing Performance 3 When the metering unit has drawn the required volume of sample into the sample loop the needle is raised and the vial is replaced in the sample tray The needle is lowered into the needle seat and the injection valve switches back to the mainpass position flushing the sample onto the column Figure 20 Mainpass Position Sample Injection Sampling Unit The sampling unit comprises three main assemblies needle drive metering device and injection valve The replacement sampling unit excludes the injection valve and metering head assemblies Needle Drive The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly The upper and lower needle positions are detected by reflection sensors on the sampling unit flex b
5. Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1120 Compact LC User Manual 161 6 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis 162 1120 Compact LC User Manual Index Index A absorbance Beer Lambert 62 accessory kit 17 adapter 73 Agilent Technologies 162 algae growth 46 algea information 155 analytical head 52 analytical head assembly parts 138 ASTM environmental conditions 9 reference and conditions 14 AUTO mode 45 autosampler EMF counters 69 introduction 48 preventive maintenance and repair 95 simple repairs 95 transprot assembly parts 96 ball screw drive 40 band width6 5nm 13 Beer Lambert law 62 Bootp amp Store 23 automatic configuration 32 initialization modes 23 service 32 storing the settings permanently 27 using default 25 using stored 24 1120 Compact LC User Manual bottle head assembly 143 buffer application 46 buffer solution 87 buffers 90 bypass 49 c caps 57 cleaning the autosampler 96 column oven 60 communications 11 composition precision 12 composition range 12 compressibility compensation 12 43 configuration with Bootp 32 configuration switches 22 control syst
6. G4287 64010 G4288 64010 G4289 64010 G4290 64010 A Solvent Selection Valve SSV Upgrade Kit part number G4280 68708 is available 6 1120 Compact LC User Manual General Information about the Agilent 1120 Compact LC System 1 Site Requirements A suitable environment is important to ensure optimal performance of the System Power Consideration The Agilent 1120 Compact LC power supply has wide ranging capabilities Consequently there is no voltage selector at the instrument WARNING Instrument is partially energized when switched off The power supply still uses some power even when the power switch on the front panel is turned OFF Repair work at the detector can lead to personal injuries e g shock hazard when the detector cover is opened and the instrument is connected to power gt To disconnect the detector from the power line unplug the power cord WARNING Incorrect line voltage at the Compact LC Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument only to the specified line voltage CAUTION In case of an emergency it must be possible to disconnect the instrument from the power line at any time Make sure to have easy access to the power cable of the instrument in order to disconnect the instrument from line gt Provide sufficient space next to the power socket of the instrument to unplug
7. LC Pump Table 3 Performance Specification Agilent 1120 Compact LC Pump Type Specification Hydraulic system Dual plunger in series pump with proprietary servo controlled variable stroke drive floating plungers and passive inlet valve Settable flow range 0 001 10 ml min in 0 001 ml min increments Flow range 0 2 10 0 ml min Flow precision lt 0 07 RSD or lt 0 02 min SD whatever is greater based on retention time at constant room temperature Flow accuracy 1 or 10 l min whatever is greater 1120 Compact LC User Manual 11 1 12 General Information about the Agilent 1120 Compact LC System Table3 Performance Specification Agilent 1120 Compact LC Pump Pressure Pressure pulsation Compressibility compensation Recommended pH range Gradient formation optional Composition Range Composition Precision Operating range 0 40 MPa 0 400 bar 0 5880 psi up to 5 ml min Operating range 0 20 MPa 0 200 bar 0 2950 psi up to 10 ml min lt 2 amplitude typically lt 1 at 1 ml min isopropanol at all pressures gt 1 MPa 10 bar User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Low pressure dual mixing gradient capability using proprietary high speed proportioning valve Delay volume 800 1100 pl dependent on back pressure 0 95 or 5 100 user selecta
8. Needle seat assy 0 17 mm i d 2 3 pl G1313 87101 12 Seat adapter G1313 43204 13 Safety flap G1313 44106 14 Flex board G1313 68715 15 Needle assembly for G1313 87101 or G1313 87103 needle seat G1313 87201 Clamp kit includes needle clamp and 2 x clamp screw G1313 68713 146 1120 Compact LC User Manual Parts for Maintenance and Repair 5 Parts for Repair Figure 43 Autosampler Sampling Unit Assembly Injection Valve Assembly Table 43 Injection Valve Assembly Item Description Part Number 1 Injection valve assembly includes items1 6 0101 0921 2 Isolation seal 0100 1852 1120 Compact LC User Manual 147 5 Parts for Maintenance and Repair Parts for Repair Table 43 Injection Valve Assembly Item Description Part Number 3 Rotor seal Vespel 0100 1853 3 Rotor seal Tefzel 0100 1849 4 Stator face 0100 1851 5 Stator head 0100 1850 6 Stator screws 1535 4857 Figure 44 Injection Valve Assembly 148 1120 Compact LC User Manual Parts for Maintenance and Repair 5 Column Oven Column Oven Assy part number G4280 60040 Figure 45 Column Oven Assy 1120 Compact LC User Manual 149 Parts for Repair Detector Optical Unit and Fan Assembly This chapter provides additional information on safety legal and web 1120 Compact LC User Manual e 6 Appendix General Safety Information 152 Solvent Information 155 Radio Interfere
9. S SY Se N 92 1120 Compact LC User Manual Preventive Maintenance and Repair 4 3 Remove the seal L ZA x I a ta f DA IUA W VAX SA Nome i We X f SA R SS I XJ y ANN A NN Ne N a gt AN Ke N NN J QW 5 Install the stator ring Ensure the pin in the stator ring is aligned with the hole in the valve body A NN 2 w d J y ee iS N i A BO 4 Install the new seal Rotor pin Stator ring Noten Needle hole 6 Install the stator head onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head Pin 1120 Compact LC User Manual 93 4 Preventive Maintenance and Repair Manual Injector 7 Secure stator head in place with the stator screws Tighten each screw alternately 4 turn until the stator head is secure 94 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Autosampler Introduction into Maintenance and Repair The autosampler is designed for easy repair The most frequent repairs such as change and needle assembly change can be done from the front of the instrument with the instrument in place in the Compact LC These repairs are described in following chapters Overwiew of procedures Procedure Typical Frequency Time Required Exchanging the needle When needle shows indication of
10. Switches The configuration switch can be accessed at the rear left side of the instrument The Compact LC is shipped with switches 7 and 8 set to ON Which means that the instrument is set to a default fix IP address 192 168 254 11 To perform any LAN configuration SW1 and SW2 must be set to OFF Table 10 Factory Default Settings Initialization Init Mode Using Default switches 7 and 8 set to ON Link Configuration Speed and duplex mode determined by auto negotiation 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 Initialization mode selection The following initialization init modes are selectable Table 11 Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default Bootp When the initialization mode Bootp is selected the compact LC tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the compact LC Therefore the parameters are lost with the next power cycle of the compact LC Bootp Active Server Parameter Figure 3 Bootp Principle Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the compact LC
11. Syringe 9301 0411 1 FTG one piece fingertight 0100 2562 1 Flow cell outlet tubing 5062 8535 1 Cross over cable 5023 0203 1 Bottle amber 9301 1450 1 Bottle transparent 9301 1420 1 Bottle head assy G1311 60003 2 Caffeine standard G4218 85000 1 Table8 Accessory Kit for G4286A G4287A Contents Description Part Number Quantity Accessory kit G4286 68705 1 Helium tubing 5m PTFE 0 057 ID 0890 1195 5m Corrugated tubing to waste re order 5 m 0890 1711 3m Wrench open end 1 4 5 16 inch 8710 0510 2 1120 Compact LC User Manual 17 2 18 Installing the Agilent 1120 Compact LC Table8 Accessory Kit for G4286A G4287A Contents Description Part Number Quantity Wrench open end 14 mm 8710 1924 1 Wrench 1 2 amp 9 16 8720 0025 1 Mounting tool 0100 1710 1 Syringe adapter 9301 1337 1 Syringe 9301 0411 1 FTG one piece fingertight 0100 2562 1 Flow cell outlet tubing 5062 8535 1 Cross over cable 5183 4649 1 Bottle transparent 9301 1420 1 Bottle head assy G1311 60003 1 Syringe LC 50pl FN 5182 9619 1 Screw cap vial clear 6ml reorder pack of 100ea 9301 1377 1 Screw caps for 6ml vials reorder pack of 100ea 9301 1379 1 PTFE silicone septa 16mm pre slit reorder pack of 100ea 5188 2758 1 Caffeine standard G4218 85000 1 Table9 Accessory Kit for G4288A G4289A Contents Description Part Number Quantity Accessory kit G4288 68705 1 Helium tubing 5m PTFE 0 057 ID 0890 1195 5m Corrugated tubing to waste re or
12. black or yellow layers on its surface or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 ml min with an open purge valve When using the pump at low flow rates for example 0 2 ml min check all 1 16 inch fittings for any signs of leaks Whenever exchanging the pump seals the purge valve frit should be exchanged too When using buffer solutions flush the system with water before switching it off Check the pump plungers for scratches when changing the plunger seals Scratched plungers will lead to micro leaks and will decrease the lifetime of the seal Pressurize the system according to the wear in procedure after changing the plunger seals Prevent blocking of solvent filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the pump This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the pump Use a sterile if possible amber solvent bottle to slow down algae growth Filter solvents through filters or membranes that remove algae Exchange solvents every two days or refilter If the application permits add 0 0001 0 001M sodium azide to the solvent Place a layer of argon on top of your solvent Avoid exposure of the solvent bottle to direct sunlight Never use the sys
13. can damage electronic boards and components gt In order to prevent damage always use an ESD protection when handling electronic boards and components 1120 Compact LC User Manual 71 4 Preventive Maintenance and Repair Checking and Cleaning the Solvent Filter WARNING Small particles can permanently block the capillaries and valves of the pump Damage of the Compact LC pump gt Always filter solvents gt Never use the pump without solvent inlet filter If the filter is in good condition the solvent will freely drip out of the solvent tube hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube Cleaning the Solvent Filter When If solvent filter is blocked Parts required Description Concentrated nitric acid 65 Bidistilled water Beaker Preparations Remove solvent inlet tube from the Inlet Valve 1 Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 65 for one hour 2 Thoroughly flush the filter with bidistilled water remove all nitric acid some capillary columns can be damaged by nitric acid 3 Replace the filter 72 1120 Compact LC User Manual Preventive Maintenance and Repair Exchanging the Passive Inlet Valve When Tools required Parts required Preparations If internally leaking backflow Wrench 14 mm flat Pair of tweezers Par
14. duplex Storing the settings permanently with Bootp If you want to change parameters of the Compact LC using the Bootp follow the instructions below 1 Turn off the Compact LC 2 Change the Compact LC s settings of the Configuration Switch to Bootp amp Store mode Start the Agilent Bootp Service and open its window 4 If required modify the parameters for the Compact LC according to your needs using the existing configuration 5 Press OK to exit the Bootp Manager 6 Now turn on the Compact LC and view the Bootp Server window After some time the Agilent Bootp Service will display the request from the LAN interface The parameters are now stored permanently in the non volatile memory of the Compact LC Close the Agilent Bootp Service and turn off the Compact LC Change the settings of the Compact LC s Configuration Switch to Using Stored mode 1120 Compact LC User Manual 27 2 28 Installing the Agilent 1120 Compact LC 9 Power cycle the Compact LC The Compact LC can be accessed now via LAN without the Agilent Bootp Service Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the Compact LC It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switche
15. or hazardous solvents are used LOAD Position In the LOAD position see Figure 22 on page 55 the pump is connected directly to the column ports 2 and 3 connected and the needle port is connected to the sample loop At least 2 to 3 sample loop volumes more if better precision is required of sample should be injected through the needle port to provide good precision The sample fills the loop and excess sample is expelled through the vent tube connected to port 6 Needle port Waste to column from pump Sample loop Figure 22 LOAD Position 1120 Compact LC User Manual 55 3 Introduction and Optimizing Performance INJECT Position In the INJECT position see Figure 23 on page 56 the pump is connected to the sample loop ports 1 and 2 connected All of the sample is washed out of the loop onto the column The needle port is connected to the vent tube port 5 Needle port F i Lua Waste 2 1 to column from pump Sample loop Figure 23 INJECT Position Using the Autosampler Supported trays for the autosampler Table 19 Supported trays for the Autosampler Description Part Number Tray for 100 x 2 ml vials G1313 44510 Halftray for 15 x 6 ml vials G1313 44513 Halftray for 40 x 2 ml vials G1313 44512 56 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Half tray combinations Half trays can be installed in any combination enabling both 2 ml and 6 ml vials to be used simul
16. oven is based on a resistor heater matt with two thermal sensors to provide constant temperature in the whole column area A build in over temperature cut off fuse inhibits overheating The inner volume of the oven capillary is 6y1 Maximum column length is 25cm 10 inch Operational range is 5 degree above ambient at least 10 C up to 60 C max specified flow rate is 5ml min at 60 C Never operate the column oven with open front cover to ensure a correct column temperature always operate with closed front cover The counterpart of the oven isolation is fixed at the inner side of the front cover 1120 Compact LC User Manual Detector Introduction and Optimizing Performance 3 Introduction to the Detector The Agilent 1120 Compact LC variable wavelength detector is designed for highest optical performance GLP compliance and easy maintenance with e Deuterium lamp for highest intensity and lowest detection limit over a wavelength range of 190 to 600 nm Optional flow cell cartridges standard 10 mm 14 ul high pressure 10 mm 14 ul micro 5 mm 1 ul semi micro 6 mm 5 ul are available and can be used depending on the application needs e Easy front access to lamp and flow cell for fast replacement and e Built in holmium oxide filter for fast wavelength accuracy verification The detector has a variety of parameters that can be used to optimize performance The information below will guide you on how to get the best dete
17. stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol 1120 Compact LC User Manual 155 6 Appendix e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid e Mixtures of carbon tetrachloride with 2 propanol or THF 156 1120 Compact LC User Manual Appendix 6 Radio Interference Cables supplied by Agilent Technoligies are screened to provide opitimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1120 Compact LC User Manual 157 6 Appendix UV Radiation Emissions of ultraviolet radiation 200 315 nm from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is li
18. the cable 1120 Compact LC User Manual 7 1 General Information about the Agilent 1120 Compact LC System Power Cords Different power cords are offered as options with the system The female end of the power cords is identical It plugs into the power input socket at the rear left side of the Compact LC The male end of each power cord is different and designed to match the wall socket of a particular country or region The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit Electric Shock gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables which haven t been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Bench Space The Compact LC dimensions and weight allow to place the instrument on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches in the rear for air circulation and electric connections If the bench should carry a Agilent 1120 Compact LC system make sure that the bench is designed
19. the user interface when the limit is exceeded The counter can be reset to zero after the lamp is exchanged The detector provides the following EMF counters e Deuterium Lamp On Time 1120 Compact LC User Manual 69 4 Preventive Maintenance and Repair Solvent Delivery System Introduction and Overview to simple repairs of the pump The Compact LC pump is designed for easy repair The procedures described in this section can be done with the pump in place in the rack The most frequent repairs such as plunger seal change and purge valve seal change can be done from the front of the pump Table 27 Simple repair procedures overview Procedure Typical frequency Notes Checking and cleaning the solvent filter Exchanging the Passive Inlet Valve Exchanging the Outlet Ball Valve Exchanging the Purge Valve Frit or the Purge Valve Exchanging the Purge Valve Frit or the Purge Valve Exchanging the Pump Seals Exchanging the Seal Wear in Procedure Removing the pump head assembly Exchanging the Plungers If solvent filter is blocked If internally leaking If internally leaking If internally leaking If the frit shows indication of contamination or blockage If pump performance indicates seal wear If pump performance indicates seal wear Before exchanging the seals or plungers If scratched Gradient performance problems intermittent pressure fluctuations Pressure ripple unstable run leak tes
20. to bear the weight of the system The Compact LC should be operated upright 1120 Compact LC User Manual General Information about the Agilent 1120 Compact LC System 1 Environment CAUTION Your Compact LC will work within specifications at ambient temperatures and relative humidity as described in the following chapters ASTM drift tests require a temperature change below 2 C hour 3 6 F hour measured over one hour period Our published drift specification is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation 1120 Compact LC User Manual 1 General Information about the Agilent 1120 Compact LC System Physical Specification Table 1 Physical Specifications Type Specification Comments Weight 30 kg 66 Ibs Dimensions height x width x depth
21. valve bypass and mainpass end positions No valve adjustments are required after replacing internal components Table 18 Injection Valve Technical Data Standard Motor type 4 V 1 2 A stepper motor 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Injection System Table 18 Injection Valve Technical Data Seal material Vespel Tefzel available Number of ports 6 Switching time lt 150 ms Transport Assembly The transport unit comprises an X axis slide left right motion a Z axis arm up down motion and a gripper assembly rotation and vial gripping Theta motor Gripper motor X motor Gripper Theta axis Flex board Figure 21 Transport Assembly The transport assembly uses four stepper motors driven in closed loop mode for accurate positioning of the gripper assembly for sample vial transport The rotational movement of the motors is converted to linear motion X and 1120 Compact LC User Manual 53 3 Introduction and Optimizing Performance Z axes by toothed belts connected to the drive spindles The rotation theta axes of the gripper assembly is transferred from the motor by a toothed belt and series of gears The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly The stepper motor positions are determined by the optical encoders mounted onto the stepp
22. 0 1922 0101 1239 Sample loop 50 pl 0100 1924 0101 1238 Sample loop 100 pl 0100 1921 0101 1242 Sample loop 200 ul 0101 1247 0101 1227 Sample loop 500 pl 0101 1246 0101 1236 Sample loop 1 ml 0101 1245 0101 1235 Sample loop 2 ml 0101 1244 0101 1234 Sample loop 5 ml 0101 1243 0101 1230 1120 Compact LC User Manual 137 5 Parts for Maintenance and Repair Autosampler Table 37 Autosampler Main Assemblies Item Description Part Number 1 Transport assembly G1329 60009 2 Sampling unit assembly G4280 60027 The assy comes without injection valve and analytical head 3 Analytical head assembly 100 pl 01078 60003 4 Injection valve assembly 0101 0921 5 Vial tray G1313 44510 6 Gripper assembly G1313 60010 Analytical Head Assembly Table 38 Analytical Head Assembly 100 pl Item Description Part Number Analytical head assembly includes items 1 6 01078 60003 1 Screws 0515 0850 2 Plunger assembly 5063 6586 3 Adapter 01078 23202 4 Support seal assembly 5001 3739 5 Metering seal pack of 2 5063 6589 6 Head body 01078 27710 7 Screw M5 60 mm lg for mounting of assembly 0515 2118 138 1120 Compact LC User Manual Parts for Maintenance and Repair 5 Parts for Injection System Maintenance Figure 38 Analytical Head Assembly 1120 Compact LC User Manual 139 5 Parts for Maintenance and Repair Column Oven Column Oven Assy part number G4280 60040 Figure 39 Column Oven Assy 140 1120 C
23. 1 G1311 60012 Outlet ball valve Switch off pump at the main power switch Remove the upper front cover Before exchanging the outlet ball valve you can try to clean it in a sonic bath for 5 10 minutes Place the valve in an upright position in a small beaker with alcohol 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet ball valve 2 Using the 14 mm wrench loosen the valve and remove it from the pump body 3 Check that the new valve is assembled correctly and that the gold seal is present if the gold seal is deformed it should be replaced 1120 Compact LC User Manual Preventive Maintenance and Repair 4 3 AA Figure 27 Outlet Ball Valve Parts Outlet ball valve complete assembly G1311 60012 1 Socket cap 5042 1345 2 Outlet valve housing screw01018 22410 3 Gold Seal 5001 3707 4 Cap pack of 4 5062 2485 4 Reinstall the outlet ball valve and tighten the valve 5 Reconnect the valve capillary 1120 Compact LC User Manual 75 4 Preventive Maintenance and Repair Solvent Delivery System Valve capillary Outlet ball valve Figure 28 Exchanging the Outlet Ball Valve 76 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Exchanging the Purge Valve Frit or the Purge Valve When Tools required Parts required Preparations Frit when plunger seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow
24. 120 Compact LC System e Installing the Agilent 1120 Compact LC e Introduction and Optimizing Performance e Preventive Maintenance and Repair e Parts for Maintenance and Repair e Appendix Agilent Technologies 2008 Printed in Germany 02 08 G4280 90000 EE Agilent Technologies
25. 1314 60200 Cuvette Holder 1 Cuvette with the standard e g NIST certified holmium oxide sample 1 G1313 87201 Needle Preparations Select Start in the Tools function in the LMD Software Change Needle When the needle is positioned approx 15 mm above the needle seat remove the upper front cover iim To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not bend the safety flap away from its position or attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle 1120 Compact LC User Manual 97 4 Preventive Maintenance and Repair Autosampler 1 Select Needle Down until the needle screw is 2 Remove the sample loop fitting from the aligned with the hole in the safety cover needle fitting 98 1120 Compact LC User Manual Preventive Maintenance and Repair Autosampler 4 3 Loosen the fixing screw 1 and lift out the needle 2 4 Select Needle Down to move the needle arm to its lowest position The needle arm must be in its lowest position before installing the new needle otherwise leaks at the needle seat will occur due to incorrect needle installation 5 Insert the new needle 1 Align the needle in the seat then tighten the screw firmly 2 6 Reconnect the sample loop fitting to the needle fitting Lt i on lt r
26. 640x370x420 mm 25 2x14 6x16 5 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 240 VA 210 W 717 BTU Maximum Ambient operating temperature 0 55 C 32 131 F Ambient non operating temperature 40 70 C 4 158 F Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For storing the instrument Safety standards IEC CSA UL EN Installation Category II Pollution Degree 2 For indoor use only Housing All materials recyclable 10 1120 Compact LC User Manual General Information about the Agilent 1120 Compact LC System 1 Performance Specifications Performance Specifications Agilent 1120 Compact LC System Table 2 Performance Specification Agilent 1120 Compact LC System Type Specification Safety features Extensive diagnostics error detection and display leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Control and data evaluation Agilent EZChrom Compact Agilent Lab Monitoring and Diagonostic Software LMD Communications Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN GLP features Early maintenance feedback EMF electronic records of maintenance and errors Performance Specifications Agilent 1120 Compact
27. Agilent 1120 Compact LC User Manual ot Agilent Technologies Notices Agilent Technologies Inc 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64280 90000 Edition 02 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreeme
28. L LN i E 5 Use Down to position the needle 6 Ensure the needle is aligned with the seat If approximately 2 mm above the seat required bend the needle slightly until the needle is aligned correctly 102 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Next Steps 7 On completion of this procedure Install the front cover 8 Select End in the Tools function Change Needle Stator Face When Poor injection volume reproducibility Leaking injection valve Tools required 1 4 inch wrench supplied in accessory kit Hex key 9 64 inch supplied in accessory kit Parts required Partnumber Description 1 0100 1851 Stator face for injection valve Preparations Remove the upper front cover 1 Remove all capillary fittings from the 2 Loosen each fixing bolt two turns at a time injection valve ports Remove the bolts from the head 1120 Compact LC User Manual 103 4 Preventive Maintenance and Repair Autosampler 3 Remove the stator head and stator face The stator face is held in place by the stator head When removing the stator head ensure the stator face does not fall out of the valve 4 Place the stator face in place on the stator head Ensure the pins on the stator engage in the holes in the stator head 5 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator he
29. Thus after a power cycle they are still available This enables a kind of bootp once configuration of the compact LC Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server 1120 Compact LC User Manual 23 2 24 Installing the Agilent 1120 Compact LC temporarily powers on the compact LC using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the compact LC Then he selects the initialization mode Using Stored and powers on the compact LC again From now on he is able to establish the TCP IP connection to the compact LC with the parameters obtained in that single Bootp cycle Bootp Server Active Parameter Non Volatile RAM Stored Parameter Figure 4 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when the compact LC shall obtain its parameters from a Bootp Server every time it is powered on the recommended initialization mode is Bootp Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the compact LC The TCP IP connection will be established using these param
30. To install the System it s highly recommended to follow the Installation Guide for Agilent 1120 Compact LC System step by step a Agilent Technologies 15 2 Installing the Agilent 1120 Compact LC Unpacking your System Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the Compact LC may have been damaged during shipment CAUTION Signs of damage gt Do not attempt to install the Compact LC Delivery Checklist Ensure all parts and materials have been delivered with the Compact LC The delivery checklist is shown below Please report missing or damaged parts to your local Agilent Technologies sales and service office Table6 Compact LC Checklist Description Quantity Compact LC 1 Power cable 1 Flow cell installed Software CD DVD 2 Installation guide 1 Accessory kit see below 1 16 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 Compact LC Accessory Kits Contents Table7 Accessory Kit for G4290A Contents Description Part Number Quantity Accessory kit G4280 68705 1 Helium tubing 5m PTFE 0 057 ID 0890 1195 5m Corrugated tubing to waste re order 5 m 0890 1711 3m Wrench open end 1 4 5 16 inch 8710 0510 2 Wrench open end 14 mm 8710 1924 1 Wrench 1 2 amp 9 16 8720 0025 1 Mounting tool 0100 1710 1 Syringe adapter 9301 1337 1
31. act LC User Manual Preventive Maintenance and Repair 4 Detector Correcting Leaks When If a leakage has occurred in the flow cell area or at the capillary connections Tools required Tissue Two 1 4 inch wrenches for capillary connections Parts required Description None 1 Remove the lower front cover 2 Use tissue to dry the leak sensor area 3 Observe the capillary connections and the flow cell area for leaks and correct if required 4 Replace the front cover Leak plane Waste outlet Leak sensor assembly Figure 32 Drying the Leak Sensor 1120 Compact LC User Manual 123 4 Preventive Maintenance and Repair Tests amp Calibrations Probable Causes 124 Suggested Actions The following tests are required after maintenance of lamps and flow cells e Intensity Test e Wavelength Verification Calibration Holmium Oxide Test Intensity Test The intensity test measures the intensity of the deuterium lamp over the full VWD wavelength range 190 600 nm The test can be used to determine the performance of the lamp and to check for dirty or contaminated flow cell windows When the test is started the gain is set to zero To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The shape of the intensity spectrum is primarily dependent on the lamp grating and diode characteristics Therefore intensity spectra will differ slightly between in
32. ad is secure ports 104 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Next Steps 7 Slide the waste tube into the waste holder in the leak tray 8 On completion of this procedure Install the front cover Exchanging the Rotor Seal When Poor injection volume reproducibility Leaking injection valve Tools required e 1 4 inch wrench supplied in accessory kit Hex key 9 64 inch supplied in accessory kit Parts required Partnumber Description 1 0100 1853 Rotor seal Vespel 1 0100 1849 Rotor seal Tefzel 1 0101 1416 Rotor seal PEEK Preparations Remove upper front cover Remove the leak tubing if necessary CAUTION Removing the stator head The stator face is held in place by the stator head When you remove the stator head the stator face can fall out of the valve gt Carefully handle the valve to prevent damage to the stator face 1120 Compact LC User Manual 105 4 Preventive Maintenance and Repair Autosampler 1 Remove all capillary fittings from the 2 Loosen each fixing bolt two turns at a time injection valve ports Remove the bolts from the head 3 Remove the stator head stator face and stator ring a 106 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Autosampler 5 Install the new rotor seal and isolation seal Ensure the meta
33. als 86 84 81 metering seal 138 seal wear in 83 setable flowrange 11 simple repairs autosampler 95 site requirements 7 solvent inlet filters 45 solvent filters checking 72 cleaning 72 solvent information 155 Solvent Selection Valve 6 solvents buffers 90 rinsing 90 salt solutions 90 sonic bath 74 specifications 12 performance 13 SSV 6 standards 10 stator 52 stepper motor 51 storing the settings permanently 27 stroke volume 45 40 T TCP IP parameter configuration 22 telnet configuration 28 temperature 12 tests holmium oxide holmium oxide test 126 intensity of lamp 124 wavelength calibration 125 theta axis 53 transport assembly 53 transport mechanism 48 165 Index U URL 162 using the cuvette holder 120 UV radiation 158 V vacuum degasser 45 valve frit 77 valve proportioning 39 variable reluctance motor 40 variable stroke volume 45 vial contents temperature 12 vial numbering 56 vial racks 48 vials 57 48 wW wavelength accuracy 13 calibration 125 range 190 600nm 13 wear in procedure 83 WEEE 162 weight 10 wrench 1 4inch 84 74 77 79 81 86 87 wrench 14mm 77 73 X X axis 53 Z Z axis 53 166 1120 Compact LC User Manual Index 1120 Compact LC User Manual 167 www agilent com In this Book This manual contains information about the Agilent 1120 Series Compact LC The manual describes the following e General Information about the Agilent 1
34. alue Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X xX gt sets new ip address SM lt X X X X gt sets new subnet mask gW lt X X X X gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 27 230 Then press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return 5 Use the and press Enter to list the current settings 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 information about the LAN interface MAC address initialization mode Initialization mode is Using Stored Init Mode Using Stored TCP IP Properties active IP Address 134 46 27 95 active TCP IP settings Subnet Mask 255 255 248 0 Def Gateway 134 490 24 1 TCP IP status here ready i m connected to PC with controller Controllers no connections software e g Agilent ChemStation here not connected Figure 11 Telnet Current settings in Using Stored mode 6 Change the IP address in this example 134 40 27 99 and type to list current settings E C WINDOWS system32 cmd_exe telnet 134 40 27 95 gt ip 134 40 27 99 change of IP setting to gt LAN Status Page 663 6D3G6AG838 Init Mo
35. alve complete assembly G4280 60031 1 Valve body No part number 2 PTFE frit pack of 5 01018 22707 3 Gold seal 5001 3707 4 Cap pack of 4 5062 2485 Figure 35 Purge Valve Assembly 134 1120 Compact LC User Manual Parts for Maintenance and Repair 5 Passive Inlet Valve Table 34 Passive Inlet Valve Item Description Part Number Passive inlet valve G4280 60005 1 O Ring 7 5 MM ID 0905 1684 2 Gold seal 5001 3707 3 Cap pack of 4 5062 2485 Y 0 Figure 36 Passive Inlet Valve Assembly 1120 Compact LC User Manual 135 5 Parts for Maintenance and Repair Parts for Injection System Maintenance 136 Injection Valve Assembly Table 35 Manual Injection Valve assembly Item Description Part Number Manual injection valve complete assembly including loop 5067 4102 capillary 20ul and needle port Manual Injection Valve excluding loop capillary and needle port 5067 4104 1 Bearing ring 1535 4859 3 Rotor seal PEEK 5067 4105 5 Stator head 0100 1850 Needle Port 5067 1581 6 Stator screws 1535 4857 7 Hex key 9 64 inch for stator screws not shown 8710 0060 1120 Compact LC User Manual Parts for Maintenance and Repair 5 A LAITA D V D Figure 37 Injection Valve Assembly Table 36 Sample loops manual injector Description Stainless PEEK loops Steel loops Sample loop 5 pl 0101 1248 0101 1241 Sample loop 10 pl 0100 1923 0101 1240 Sample loop 20 pl 010
36. apted to specific user requirements The wear of pump components is dependent on the analytical conditions Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument The Compact LC pump provides a series of EMF counters for the pump head Each counter increments with pump use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The quaternary pump provides the following EMF counters Pump liquimeter e Pump seal wear Pump liquimeter The pump liquimeter displays the total volume of solvent pumped by the pump head since the last reset of the counters The pump liquimeter can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed Seal wear counters The seal wear counters display a value derived from pressure and flow both contribute to seal wear The values increment with pump usage until the counters are reset after seal maintenance Both seal wear counters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed 68 1120 Compact LC User Manual Preventive Maintenance and Repair 4 EMF Counters for the autosampler The user setable EMF limits for the EMF counters enable the early maintenance feedback to be adap
37. ase 1 Apply a small amount of grease on the back of the pistons 2 Slide the Pumphead Assembly onto the Pumpdrive 3 Using a 4 mm hexagonal key tighten the Pumphead screws stepwise with increasing torque Pump head screws 4 Reconnect the capillaries and tubing 5 Reinstall the front cover 86 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Exchanging the Dual Channel Gradient Valve DCGV Tools required Pozidriv 1 Parts required Partnumber Description 1 G4280 60004 Dual channel gradient valve Preparations Switch the pump off at the power switch Remove the upper front cover to have access to the pump mechanics The lifetime of the dual channel gradient valve can be maintained by regularly flushing the valve especially when using buffer solutions If using buffer solutions flush all channels of the valve with water to prevent precipitation of the buffer Salt crystals can be forced into an unused channel and form plugs that may lead to leaks of that channel Such leaks will interfere with the general performance of the valve When using buffer solutions and organic solvents in the Agilent 1120 Compact LC Pump it is recommended to connect the buffer solution to the bottom port and the organic solvent to the upper gradient valve port It is best to have the organic channel directly above the salt solution channel A salt solution B organic solvent 1120 Compact LC User Manual 87 4 Prevent
38. ble lt 0 2 RSD at 0 2 and 1 ml min Performance Specifications Agilent 1120 Compact LC Autosampler Table 4 Performance Specifications Agilent 1120 Compact LC Autosampler Type Pressure Injection range Replicate injections Precision Minimum sample volume Carryover Sample viscosity range Specification Operating range 0 40 MPa 0 400 bar 0 5900 psi 0 1 100 pl in 0 1 pl increments Up to 1500 pl with multiple draw hardware modification required 1 99 from one vial lt 0 25 RSD from 5 100 ul lt 1 RSD 1 5 ul variable volume 1 ul from 5 pl sample in 100 pl microvial or 1 pl from 10 pl sample in 300 pl microvial Typically lt 0 1 lt 0 05 with external needle cleaning 0 2 50 cp 1120 Compact LC User Manual General Information about the Agilent 1120 Compact LC System 1 Table4 Performance Specifications Agilent 1120 Compact LC Autosampler Sample capacity 100 x 2 ml vials in 1 tray 40 x 2 ml vials in tray 15 x 6 ml vials in tray Agilent vials only Injection cycle time Typically 50 s depending on draw speed and injection volume Performance Specifications Agilent 1120 Compact LC VWD Table5 Performance Specifications Agilent 1120 Compact LC Variable Wavelength Detector Type Specification Comments Detection type Double beam photometer Light source Deuterium lamp Wavelength range 190 600 nm Short term noise 0 5 x 10 5 AU at 254 nm S
39. crew two revolutions and housing and lift the housing away from the while holding the lower half of the assembly plungers carefully pull the pump head away from the plunger housing w mae aS Support ring Ih a Plunger housing lt 1120 Compact LC User Manual 81 4 Preventive Maintenance and Repair Solvent Delivery System 3 Using one of the plungers carefully remove 4 Insert new seals into the pump head the seal from the pump head be careful not to break the plunger Remove wear Seals retainers if still present Pump head Support ring Plunger housing 82 1120 Compact LC User Manual CAUTION Preventive Maintenance and Repair 4 Seal Wear in Procedure This procedure is required for standard seals only 5063 6589 It will damage the normal phase application seals 0905 1420 gt Never perform the seal wear in procedure with normal phase application seals Place a bottle with 100 ml of Isopropanol in the solvent cabinet and place a tubing including bottle head assembly in the bottle 2 Connect the inlet tube from the bottle head directly to the Inlet Valve 3 Connect the restriction capillary 5022 2159 to the purge valve Insert its other end into a waste container Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 ml min Close the purge valve set the flow to a rate adequate to achieve a pressur
40. ctive TCP IP parameters immediately and the TCP IP connection can be established Configuring the Agilent Bootp service program All examples shown in this chapter will not work in your environment You need your own IP Subnet Mask and Gateway addresses Assure that the Compact LC configuration switch is set properly The setting should be either Bootp or Bootp amp Store 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 Assure that your instrument is powered off If the Agilent Bootp Service program is not already installed on your PC then install it from your software CD ROM located in folder Bootp 1 The Agilent Bootp Service is placed in the start up group and automatically is started during the boot process of the PC 2 Open the Bootp Settings window and enter the default settings for your setup 3 Launch the Manager It will open the Bootp Manager screen This shows all network hardware that has been added initially empty 4 Select Add to enter the enter the module specific information MAC address from label on the instrument host name IP address comment instrument name location subnet mask if different gateway if required 5 Press OK The parameter are added to the Bootp Manager and added to the TabFile 6 Press Exit Manager and OK to exit the Agilent Bootp Service 7 Now turn on the instrument wait about 30 60 seconds and view the LogFile see Figure below It should disp
41. ctor performance Follow these rules as a start for new applications It gives a rule of thumb for optimizing the detector parameters Match the Flow Cell to the Column The Figure below recommends the flow cell that matches the column used If more than one selection is appropriate use the larger flow cell to get the best detection limit Use the smaller flow cell for best peak resolution 1120 Compact LC User Manual 61 3 Introduction and Optimizing Performance Detector Column length Typical peak Recommended flow cell width lt 5cm 0 025 min Standard flow cell E Typical flow 0 2 ml min 0 2 0 4 ml min 0 4 0 8 ml min 1 5 ml min rate Internal column diameter ftom fam 3 0 mm 4 6mm Figure 24 Choosing a Flow Cell Flow Cell Path Length Lambert Beer s law shows a linear relationship between the flow cell path Absorbance log T log _e C d where T isthe transmission defined as the quotient of the intensity of the transmitted light I divided by the intensity of the incident light Ip e is the extinction coefficient which is a characteristic of a given substance under a precisely defined set of conditions of wavelength solvent temperature and other parameters C is the concentration of the absorbing species usually in g l or mg 1 and d is the path length of the cell used for the measurement 62 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Therefore flow cell
42. damage 15 minutes assembly or blockage Exchanging the seat assembly When the seat shows indication of damage 10 minutes or blockage Exchanging the rotor seal After approximately 30000 to 40000 30 minutes injections or when the valve performance shows indication of leakage or wear Exchanging the metering seal When autosampler reproducibility 30 minutes indicates seal wear Exchanging the gripper arm When the gripper arm is defective 10 minutes WARNING The power supplies still use some power even if the power switch on the front panel is turned off Repair work at the autosampler can lead to personal injuries e g shock hazard when the autosampler cover is opened and the instrument is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed 1120 Compact LC User Manual 95 4 Preventive Maintenance and Repair Exchanging internal parts Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the autosampler unit out of the Compact LC these repairs have to be done by trained service personal only Safety flap flex board It is strongly recommended that the exchange of the safety flap and flex board is done by Agilent trained service personnel Transport assembly parts The ad
43. de ing Stared Initialization mode is Using Stored TCP IP Properties active IP Address 134 40 27 95 i 7 Subnet Mask 255 255 248 0 active NEEM settings Def Gateway z 134 46 24 1 stored IP Addre gt 134 40 27 99 Def Gatousy er z an oad stored TCP IP settings in non volatile memory connected to PC with controller no connections software e g Agilent ChemStation here not connected Figure 12 Telnet Change IP settings 7 When you have finished typing the configuration parameters type exit and press Enter to exit with storing parameters 1120 Compact LC User Manual 31 2 32 Installing the Agilent 1120 Compact LC ot c WINDOWS system32 cmd exe Agilent Technologies G4296A DEGOHHHHHHH gt exit Connection to host lost C N Figure 13 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 134 40 27 99 Automatic configuration with Bootp When automatic configuration with Bootp is selected and the LAN interface is powered on it broadcasts a BOOTP Bootstrap Protocol request that contains its MAC hardware address A BOOTP server daemon searches its database for a matching MAC address and if successful sends the corresponding configuration parameters to the compact LC as a BOOTP reply These parameters become the a
44. ded operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided 1120 Compact LC User Manual Appendix 6 CAUTION Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrum
45. der 5 m 0890 1711 3m Wrench open end 1 4 5 16 inch 8710 0510 2 Wrench open end 14 mm 8710 1924 1 Wrench 1 2 amp 9 16 8720 0025 1 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 Table9 Accessory Kit for G4288A G4289A Contents Description Part Number Quantity Mounting tool 0100 1710 1 Syringe adapter 9301 1337 1 Syringe 9301 0411 1 FTG one piece fingertight 0100 2562 1 Flow cell outlet tubing 5062 8535 1 Cross over cable 5183 4649 1 Bottel amber 9301 1450 1 Bottle transparent 9301 1420 1 Bottle head assy G1311 60003 2 Syringe LC 50pl FN 5182 9619 1 Screw cap vial clear 6ml reorder pack of 100ea 9301 1337 1 Screw caps for 6ml vials reorder pack of 100ea 9301 1379 1 PTFE silicone septa 16mm pre slit reorder pack of 100ea 5188 2758 1 Caffeine standard G4218 85000 1 1120 Compact LC User Manual 19 2 Installing the Agilent 1120 Compact LC LAN Configuration What you have to do first The Agilent 1120 Compact LC has an on board LAN communication interface 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear left side of the Instrument next to the configuration switch Part number of the detector G42 0 61000 main board Revision Code 26 ZZ 747 3 Vendor Year and Wee
46. e of 350 bar Pump 15 minutes at this pressure to wear in the seals Turn OFF the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reinstall the bottle with the solvent for your application Rinse your system with the solvent used for your next application 1120 Compact LC User Manual 83 4 Preventive Maintenance and Repair Exchanging the Plungers When When scratched Wrench 1 4 inch 4 mm hexagonal key Tools required Parts required Partnumber Description 1 5063 6586 Plunger Preparations Remove the upper front cover Switch off pump at the main power switch 1 Disassemble the Pumphead assembly g m ring Plunger housing Plunger gt Check the plunger surface and remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace plunger if scratched Y Plunger surface LA x eT a ee X A AN 84 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Solvent Delivery System 3 Reassemble the Pumphead assembly o T Pump head m Support ring Plunger housing Nee _ 1120 Compact LC User Manual 85 4 Preventive Maintenance and Repair Solvent Delivery System Reinstalling the Pump Head Assembly When When reassembling the pump Tools required 4 mm hexagonal key Parts required Description 79846 65501 Pump head gre
47. e head assembly 143 1120 Compact LC User Manual Index hydraulic path 144 outlet ball valve 133 passive inlet valve 135 pump head 131 performance specifications autosampler 12 performance specifications 13 pHrange 12 photometric accuracy 63 physical specifications humidity 10 line voltage and frequency 10 operating temperature 10 power consumption 10 safety information 10 weight and dimensions 10 piston chamber 39 piston 46 84 40 PM 67 power consumption 10 precision 55 pressure pulsation 12 45 43 pressure operating range 12 pressure 39 preventive maintenance 67 proportioning valve high speed 39 PTFE frit 77 pump head assembly 131 pump failure 36 pump piston 46 pump functional principle 40 hints for successful use 45 urge valve frit 46 ge urge valve 77 ge 1120 Compact LC User Manual radio interference 157 reassembling the pump head 86 recommended pH range 12 reference conditions 14 removing pump head assembly 79 repair procedures injection seal 92 repairs metering plunger 109 metering seal 109 needle assembly 97 needle seat assembly 101 overview of simple repairs 116 rotor seal 105 stator face 103 rotor seal exchange 105 S safety class 152 safety features system 11 safety general information 152 152 salt solutions 90 sample trays 56 numbering of vial positions 57 sample volume 55 sampling sequence 48 sampling unit 51 sapphire piston 40 40 seal wear counters 68 se
48. e pump input Delay volume 800 1100 pl depends on back pressure Materials in contact with mobile phase DCGV PEEK PFA Pump head SST gold sapphire ceramic Inlet valve SST gold sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby Adapter SST gold Purge valve SST gold PTFE ceramic PEEK 42 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Compressibility Compensation How does compressibility compensation work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example ageing of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first plunger The pressure in the plunger chamber increases and the volume in the chamber will be compressed depending on backpressure and solvent type The volume displaced into the system will be reduced by the compressed volume With a compressibility value set the processor calculates a compensation volume that is depending on the backpressure in the system and the selected compressibility This compensation volume will be added to the normal stroke volume and c
49. ectrical connectors are placed on the rear left side of the instrument Available connectors Power connector female end LAN connector Compact LC to controlling PC CAN connectors Compact LC to additional Agilent 1200 Series Module USB connector for future use RS232 connector APG Remote connector 12V DC Output 1120 Compact LC User Manual 37 3 Introduction and Optimizing Performance e 8 bit configuration switch see chapter LAN configuration e 5 Main Board fuses 250Vac T3 15A part number 2110 1417 Fuse F1 Degasser Pump Injector Motors Fuse F2 Injector sensors Column Oven Ext 24V Connector Fuse F3 Processor Core 5V 15V 15V supply on Mainboard Fuse F4 VWD incl D2 Lamp Fuse F5 VWD Heater FAN Next to each fuse is a LED Red LED indicates the fuse is blown If one of the fuses is blown the green LED of the power switch is flashing e Fuse Netfilter 250Vac T1OAH part number 2110 1004 38 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Solvent Delivery Systems This chapter provides an overview on the operational principles of the Solvent Delivery System Pump and optional Degasser Overview of the solvent delivery system The pump is based on a two channel dual plunger in series design which comprises all essential functions a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by one pump assembly whic
50. ee NOTE below the table ASTM Drift 3 x 10 4 AU hr at 254 nm See NOTE below the table Linearity gt 2 AU 5 upper limit See NOTE below the table Wavelength 1nm Self calibration with accuracy deuterium lines verification with holmium oxide filter Band width 6 5 nm typical Flow cells Standard 14 ul volume 10 mm cell path Can be repaired on component length and 40 bar 588 psi pressure level maximum High pressure 14 pl volume 10 mm cell path length and 400 bar 5880 psi pressure maximum Semi micro 5 pl volume 6 mm cell path length and 40 bar 588 psi pressure maximum 1120 Compact LC User Manual 13 1 General Information about the Agilent 1120 Compact LC System Performance Specifications NOTE ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions cell path length 10 mm response time 2 s flow 1 ml min LC grade methanol Linearity measured with caffeine at 272 nm nm 14 1120 Compact LC User Manual NOTE 1120 Compact LC User Manual 2 Installing the Agilent 1120 Compact LC Unpacking your System 16 LAN Configuration 20 What you have todo first 20 TCP IP parameter configuration 22 Configuration Switches 22 Initialization mode selection 23 Link configuration selection 26 Storing the settings permanently with Bootp 27 Manual Configuration 28 This chapter provides an overview on shipment content and installation
51. em 536 4r0m The variation in Measured Wavelength depends on the different Optical Bandwidth Agilent Technologies guarantees the trace ability of the specified absorb ance maxima to a National Instinute of Standards amp Technology NIST Hohmann Oxide Sobstion Qandard vrith a lot to lot tolerance of 0 3 ram The vravelength calibration filter built into the Agilent Technologies UV VIS detectors is made of this material and meets these specifications E is therefore suitable for wavelength calibration of these detectors within the specified wavelength accuracy of the respective dete ctor over its vravelength range Jarorary 13 2006 BAT 6o35 e0001 _ Revision E st Agilemt Technologies INURE CUNO ONIONI INNIN Effective by Jan 13 2006 Epa 160 1120 Compact LC User Manual Appendix 6 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and
52. em 11 correction factors for flow cells 63 counter autosampler 69 injection valve 69 liter 68 needle movement 69 seal wear 68 cuvette holder 120 D data evaluation 11 declaration of conformity 160 degasser 39 delay volume 42 delivery checklist 16 detection type 13 detector features 61 introduction 61 dimensions 10 drift 13 dual plunger in series design 39 dual channel gradient valve 87 E Early maintenance feedback 11 electrostatic discharge ESD 71 exchanging dual channel gradient valve DCGV 87 injection seal 92 outlet ball valve 74 passive inlet valve 73 pistons 84 purge valve frit 77 purge valve 7 7 F flow cell correction factors 63 types anddata 13 flow precision 11 11 flowrange 11 flow unstable 36 flushing the manual injector 90 G GLP features 11 gradient formation 12 gradient valve DCGV 87 163 Index gripper fingers 53 gripper arm repair 113 gripper 53 half trays 56 hexagonal key 4mm 84 hexagonal key 4mm 86 79 81 holmium oxide declaration of conformity 160 hydraulic path 144 hydraulic system 11 increased system pressure 37 information on UV radiation 158 on cuvette holder 120 onsolvents 155 initialization mode selection 23 initialization pump 41 INJECT 56 injection seal 92 injection sequence 49 injection valve 51 48 52 inlet valve 73 parts 135 installation accessory kit 17 delivery checklist 16 site requirements 7 installing the autosam
53. ent has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1120 Compact LC User Manual 153 6 Appendix Safety Symbols Table 44 Safety Symbols Symbol Description A f A The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have
54. er 20 pm 5041 2168 1120 Compact LC User Manual 143 5 Parts for Maintenance and Repair Figure 41 Bottle Head Assembly Parts Hydraulic Path Table 41 Hydraulic Path Item Description Part Number 1 Outlet capillary pump to injector device G1312 67305 Bottle head assembly bottle to PIV or vacuum degasser G1311 60003 2 Solvent tube vacuum degasser to DCGV G4280 60034 3 Capillary plunger 1 to damper G4280 67301 4 Capillary damper to plunger 2 G4280 67300 5 Connecting tube DCGV to PIV G4280 67304 6 Waste tube reoder pack 5 m 5062 2461 144 1120 Compact LC User Manual 5 Parts for Maintenance and Repair Parts for Repair Hydraulic Flow Path of the Pump Figure 42 145 1120 Compact LC User Manual 5 Parts for Maintenance and Repair Injection System Sampling Unit Assembly Table 42 Autosampler Sampling Unit Assembly Item Description Part Number Sampling unit assembly G4280 60008 The assy comes without injection valve and analytical head 1 Sampling unit connector board SUD G1313 66503 2 Belt gear for metering unit and needle arm 1500 0697 3 Stepper motor for metering unit and needle arm 5062 8590 4 Loop capillary 100 pl 01078 87302 5 Analytical head assembly 100 ul 01078 60003 6 Inj valve anal head cap 160 mm 0 25 mm G1313 87301 7 Safety cover G1329 44115 8 Injection valve assembly 0101 0921 9 Waste tube injection valve assy 120 mm G1313 87300 11
55. er motor housing The encoders monitor the position of the motors continually and correct for position errors automatically e g if the gripper is accidentally moved out of position when loading vials into the vial tray The initialization positions of the moving components are sensed by reflection sensors mounted on the flex board These positions are used by the processor to calculate the actual motor position An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly Using the Manual Injector CAUTION The Injection Seal The manual injector is supplied with a PEEK injection seal as standard Needles Needle can damage valve gt Always use the correct size needle 54 The manual injector is not supplied with syringes or needles Use needles with 0 028 inch outer diameter 22 gauge x 2 inch long needle without electro taper and with 90 point style square tip 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Injecting Sample WARNING Ejection of mobile phase When using sample loops larger than 100 pl mobile phase may be ejected from the needle port as the mobile phase in the sample loop decompresses gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic
56. es Detector not calibrated e Dirty or defective flow cell e Dirty or defective holmium oxide filter e Optical misalignment Suggested wv Re calibrate the detector Actions V Repeat the test with the flow cell removed If the test is OK exchange the flow cell components Y Run the holmium oxide filter test If the test fails exchange the filter assembly Y Realign the optical components 1120 Compact LC User Manual 127 4 Preventive Maintenance and Repair Detector 128 1120 Compact LC User Manual 1120 Compact LC User Manual e 5 Parts for Maintenance and Repair Parts for Compact LC System 130 Parts for Solvent Delivery System Maintenance 131 Pump Head Assembly 131 Outlet Ball Valve Assembly 133 Purge Valve Assembly 134 Passive Inlet Valve 135 Parts for Injection System Maintenance 136 Parts for Injection System Maintenance 136 Autosampler 138 Column Oven 140 Parts for Detector Maintenance 141 Standard Flow Cell 141 DetectorLamp 142 Parts for Repair 143 Degasser Unit 143 Solvent Delivery System 143 Injection System 146 Column Oven 149 Detector 150 This chapter provides information about Assemblies and Part Numbers a Agilent Technologies 129 5 Parts for Maintenance and Repair Parts for Compact LC System Table 30 System part numbers Description Part Number Power supply 0950 4997 Compact LC mainboard G4280 65000 Cabinet kit G4280 68703 Mainboard fuses 3 15AT 2110 1417 Netf
57. eters The parameters were configured previously by one of the described methods 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 Stored Parameter Parameter Figure 5 Using Stored Principle Using Default When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration Default Parameter Figure 6 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immediately 1120 Compact LC User Manual 25 2 26 Installing the Agilent 1120 Compact LC Table 12 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the compact LC must reside in the same subnet The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the compact LC He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the compact LC is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network t
58. fully understood and met the indicated conditions 154 1120 Compact LC User Manual Appendix 6 Solvent Information Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Prevent any crystallization of buffer solutions This will lead into a blokkage damage of the flow cell If the flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents e g Acetonitrile or Methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents e Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on e High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly Ox gt 2COCly 2HC1 This reaction in which
59. gg Agilent Technologies 35 3 Introduction and Optimizing Performance Overview of the Compact LC system 36 Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental problems associated with algae Algae deposit and grow everywhere within the HPLC system causing e Deposits on ball valves inlet or outlet resulting in unstable flow or total failure of the pump e Small pore solvent inlet filters to plug resulting in unstable flow or total failure of the pump e Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure e Column filters to plug giving high system pressure e Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common Symptoms observed with the Agilent 1120 Compact LC In contrast to the HP 1090 and HP 1050 Series HPLC systems which use helium degassing algae have a better chance to grow in systems such as the Agilent 1120 Compact LC where heli
60. glass 2 5182 0545 5183 4506 5183 4509 write on spot Table 22 Screw Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0714 5183 2067 5183 2070 Clear glass 2 5182 0715 5183 2068 5183 2071 write on spot Amber glass 2 5182 0716 5183 2069 5183 2072 write on spot Table 23 Crimp Caps Description Septa 100 Pack Silver aluminum Clear PTFE red rubber 5181 1210 Silver aluminum Clear PTFE red rubber 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber 5181 1215 Green aluminum Clear PTFE red rubber 5181 1216 Red aluminum Clear PTFE red rubber 5181 1217 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Table 24 Snap Caps Description Septa 100 Pack Clear polypropylene Clear PTFE red rubber 5182 0550 Blue polypropylene Clear PTFE red rubber 5182 3458 Green polypropylene Clear PTFE red rubber 5182 3457 Red polypropylene Clear PTFE red rubber 5182 3459 Table 25 Screw Caps Description Septa 100 Pack Blue polypropylene Clear PTFE red rubber 5182 0717 Green polypropylene Clear PTFE red rubber 5182 0718 Red polypropylene Clear PTFE red rubber 5182 0719 Blue polypropylene Clear PTFE silicone 5182 0720 Green polypropylene Clear PTFE silicone 5182 0721 Red polypropylene Clear PTFE silicone 5182 0722 1120 Compact LC User Manual 59 3 Introduction and Optimizing Performance Column Oven 60 Introduction to the column oven The column
61. h can generate pressure up to 400 bar Degassing of the solvents is done by a vacuum degasser and solvent compositions are generated on the low pressure side by a high speed proportioning valve The dual channel gradient pump includes a build in dual channel online vacuum degasser The isocratic pump goes in the Compact LC without a degasser The pump assembly includes a pump head with an inlet valve and an outlet valve A damping unit is connected between the two plunger chambers A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head The Degasser The dual channel gradient pump comes with a build in online degasser The degasser is switched on automatically when the pump is switched on even if the flow is set to 0 ml min In the vacuum chamber of the two channels a constant vacuum of 75 Torr 100 mbar is created The solvent flows through a Teflon AF tube with internal volume of 1 5 ml channel inside the vacuum chamber 1120 Compact LC User Manual 39 3 40 Introduction and Optimizing Performance How does the pump work The liquid runs from the solvent reservoir through the degasser to the DCGV and from there to the inlet valve The pump assembly comprises two substantially identical plunger chamber units Both plunger chamber units comprise a ball screw drive and a pump head with one sapphire plunger for reciprocating movement in it A servo controlled variable reluctance m
62. he user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the compact LC are not cleared automatically If not changed by the user they are still available when switching back to the mode Using Stored Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 If the compact LC is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the compact LC Table 13 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF manually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full
63. iew of Maintenance 90 Flushing the manual injector 90 Injection Valve Seal 92 Autosampler 95 Introduction into Maintenance and Repair 95 Exchanging the Needle Assembly 97 Needle Seat Assembly 101 Stator Face 103 Exchanging the Rotor Seal 105 Metering Seal and Plunger 109 Gripper Arm 113 Detector 116 ott Agilent Technologies 65 4 66 Preventive Maintenance and Repair Overview of VWD Maintenance 116 Exchanging a Lamp 116 Exchanging a Flow Cell 119 Using the Cuvette Holder 120 Correcting Leaks 123 Tests amp Calibrations 124 Preventive Maintenance PM is an Agilent Technologies recommended proce dure designed to reduce the likelihood of electro mechanical failures Failure to perform preventive maintenance may reduce the long term reliability of your 1120 Compact LC 1120 Compact LC User Manual Preventive Maintenance and Repair Preventive Maintenance Scope of Work and Checklist Preventive Maintenance Scope of Work and Checklist To perform a preventive maintenance PM the PM Scope of Work and PM checklist has to be followed step by step The PM Scope of Work and PM Checklist documents can be found in the on the DVD for the Lab Monitoring and Diagnostic LMD Software 1120 Compact LC User Manual 4 67 4 Preventive Maintenance and Repair Early Maintenance Feedback EMF EMF counters for the pump The user settable EMF limits for the EMF counters enable the early maintenance feedback to be ad
64. ilter fuses 10AT 2110 1004 130 1120 Compact LC User Manual Parts for Solvent Delivery System Maintenance Parts for Maintenance and Repair Part Number 5 Pump Head Assembly Table 31 Pump Head Assembly Item Description Complete assembly including items marked with 1 Sapphire plunger 2 Plunger housing including spring 3 Support ring 4 Seal pack of 2 or Seal pack of 2 for normal phase applications 5 Pump chamber housing 6 Passive inlet valve 7 Outlet ball valve 8 Screw lock 9 Purge valve assembly 10 Screw M5 60 mm lg G1311 60004 5063 6586 G1311 60002 5001 3739 5063 6589 0905 1420 G1311 25200 G4280 60005 G1311 60012 5042 1303 G4280 60031 0515 2118 1120 Compact LC User Manual 131 5 Parts for Maintenance and Repair Parts for Solvent Delivery System Maintenance 10 Figure 33 Pump head assembly 132 1120 Compact LC User Manual Outlet Ball Valve Assembly Table 32 Outlet Ball Valve Assembly Item Description Parts for Maintenance and Repair 5 Part Number Outlet ball valve complete assembly Socket cap Outlet valve housing screw Gold seal outlet Si wl N Cap pack of 4 G1311 60012 5042 1345 01018 22410 5001 3707 5062 2485 Figure 34 Outlet Ball Valve Assembly 1120 Compact LC User Manual 133 5 Parts for Maintenance and Repair Purge Valve Assembly Table 33 Purge Valve Assembly Item Description Part Number Purge v
65. ired Straightened paper clip Part number 1 61313 60010 Parts required Description Preparations Remove the upper front cover Gripper assembly Preventive Maintenance and Repair 4 Autosampler Select Start in the Tools function in the LMD Software ChangeGripper Turn off the power to the Instrument 1 Identify the slit below the gripper motor and the gripper arm release button 2 Rotate the arm approximately 2 5cm 1 inch to the left and insert the straightened paper clip into the slit 2 WH NG S gt mD DOD 1120 Compact LC User Manual 113 4 Preventive Maintenance and Repair Autosampler 3 Rotate the gripper arm slowly from left to 4 Hold the paper clip in place press the right and apply a gentle pressure to the gripper release button and rotate the paper clip The clip will engage on an gripper arm to the right internal catch and the rotation of the arm will be blocked 114 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Autosampler 5 The gripper arm will come off 6 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left Next Steps 7 On completion of this procedure Install the front cover 8 Turn the power to the Instrument ON 1120 Compact LC User Manual 115 4 Preventive Maintena
66. isconnect and replace the lamp Insert fix and reconnect the lamp 1120 Compact LC User Manual 117 4 Preventive Maintenance and Repair Detector 4 Replace the lamp cover Next Steps 5 Replace the front cover 6 Reset the lamp counter as described in the LMD Software documentation 7 Turn the lamp ON 8 Give the lamp more than 10 minutes to warm up 9 Perform Wavelength Calibration to check the correct positioning of the lamp 118 1120 Compact LC User Manual Exchanging a Flow Cell Preventive Maintenance and Repair Detector When If application needs a different type of flow cell or the flow cell needs repair Tools required Two 1 4 inch wrenches for capillary connections Preparations Turn the lamp OFF 1 Press the release buttons and remove the lower front cover to have access to the flow cell area 2 Disconnect the inlet and outlet capillaries 3 Unscrew the thumb screws parallel and remove the flow cell 4 Replace the flow cell and fix the thumb screws Reconnect the inlet and outlet capillaries to the flow cell 1120 Compact LC User Manual 4 Preventive Maintenance and Repair Next Steps 5 Replace the front cover To check for leaks establish a flow and observe the flow cell outside of the cell compartment and all capillary connections 7 Insert the flow cell Check the correct positioning of the flow cell 9 Re
67. ive Maintenance and Repair Solvent Delive am 1 Disconnect the connecting tube waste tube 2 Press the lower sides of the cover to unclip and the solvent tubes from the DCGV unclip it Remove the cover them from the tube clips and place them into the solvent cabinet to avoid flow by hydrostatic pressure Solvent tubes Connecting tube DCGV to PIV 3 Disconnect the DCGV cable unscrew the two 4 Place the new DCGV into position Make holding screws and remove the valve sure that the valve is positioned with the A channel at the bottom right position Tighten the two holding screws and connect the cable to its connector Holding screw Channel A 88 1120 Compact LC User Manual Preventive Maintenance and Repair 4 5 Replace the DCGV cover Reconnect the waste funnel with the waste tube holder in the top cover Insert waste tube in the holder in the waste pan and clip tube to the DCGV cover 6 Reconnect the tube from the inlet valve to the middle position of the DCGV and then the solvent tubes at channel A and B of the DCGV Solvent tubes Connecting tube DCGV to PIV 1120 Compact LC User Manual 4 Preventive Maintenance and Repair Manual Injector Overview of Maintenance Table 28 Overview of Repair Procedures Procedure Typical Frequency Time Required Flushing the injector Exchanging the stator face Exchanging the injecti
68. justment of the motors and the tension on the drive belts are important for correct operation of the transport assembly It is strongly recommended that exchange of drive belts and the gripper assembly is done by Agilent trained service personnel There are no other field replaceable parts in the transport assembly If any other component is defective flex board spindles plastic parts the complete unit must be exchanged Cleaning the autosampler Electrical shock hazard Liquid dripping into the autosampler could cause shock hazard and damage to the autosampler gt Drain all solvent lines before opening any fittings 96 The autosampler covers should be kept clean Clean with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth that liquid can drip into the autosampler Maintenance Functions Certain maintenance procedures require the needle arm metering device and gripper assembly to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Exchanging the Needle Assembly When When the needle is visibly damaged When the needle is blocked Tools required inch wrench supplied in accessory kit 2 5 mm Hex key 8710 2412 A pair of pliers Parts required Partnumber Description 1 G
69. k MAC 003003121040 of assembly MAC address Made in Germany oN Country of Origin Figure 1 MAC Label 2 Connect the instrument s LAN interface to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable 20 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 LAN Configuration Ki A maer ete Ora lt somone sapen a ae r bei iiaggna fo xe Figure 2 Location of LAN interface and MAC label 1120 Compact LC User Manual 21 2 22 Installing the Agilent 1120 Compact LC TCP IP parameter configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask e Default Gateway The TCP IP parameters can be configured by the following methods e by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol e by manually setting the parameters using Telnet The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch Configuration
70. l spring inside the isolation seal faces towards the valve body 6 Install the stator ring with the short of the two pins facing towards you at the 12 O Clock position Ensure the ring sits flat on the valve body 7 Place the stator face in place on the stator head 8 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator head is secure 1120 Compact LC User Manual 107 4 Preventive Maintenance and Repair Autosampler 9 Reconnect the pump capillaries to the valve Next Steps ports _ 10 Slide the waste tube into the waste holder in the leak tray 11 On completion of this procedure Install the front cover 108 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Metering Seal and Plunger When Poor injection volume reproducibility Leaking metering device Tools required 1 4 inch wrench supplied in accessory kit 4 mm hex key supplied in accessory kit 3 mm hex key supplied in accessory kit Parts required Partnumber Description 1 5063 6589 Metering seal pack of 2 for 100 pl analytical head 1 5063 6586 Metering plunger for 100 pl analytical head Preparations Select Start in the Tools function in the LMD Software Change piston Remove the upper front cover head faces upwards 1 Remove the two capillaries from the 2 Remove the two fixing bolts and pull the meteri
71. lay the request from the detector with the hardware MAC address 1120 Compact LC User Manual 33 2 34 Installing the Agilent 1120 Compact LC 02 03 05 16 33 56 PM Status BOOTP Request received at outer most layer Status BOOTP Request received from hardware addfess 0030D30A0838 Status found 134 40 27 95 WADI1171 Status Host IP Address is 134 40 29 56 Status Reply to BOOTP Request has been sent Status BOOTP Request finished processing at outer most layer Figure 14 LogFile the detector has received the parameter When using this Bootp mode the parameters are not written into the non volatile memory of the detector 1120 Compact LC User Manual 1120 Compact LC User Manual TP ee 3 Introduction and Optimizing e Performance 8 Overview of the Compact LC system 36 Algae Growth in HPLC Systems 36 Compact LC electronics 37 Solvent Delivery Systems 39 Overview of the solvent delivery system 39 The Degasser 39 How does the pump work 40 Compressibility Compensation 43 How does variable stroke volume work 45 Using the Pump 45 Injection System 47 Introduction to the Manual Injector 47 Introduction to the Autosampler 48 Using the Manual Injector 54 Using the Autosampler 56 Column Oven 60 Introduction to the column oven 60 Detector 61 Introduction to the Detector 61 This chapter provides general information about the use and functionality of the Compact LC System and its components A
72. mited to the following TLVs Threshold Limit Values according to the American Conference of Governmental Industrial Hygienists Table 45 UV Radiation Limits Exposure day Effective Irradiance 8 hours 0 1 pW cm 2 10 minutes 5 0 W cm Typically the radiation values are much smaller than these limits Table 46 UV Radiation Typical Values Position Effective Irradiance Lamp installed 50 cm distance average 0 016 W cm Lamp installed 50 cm distance maximum 0 14 pW cm 158 1120 Compact LC User Manual Appendix 6 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure L lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1120 Compact LC User Manual 159 6 Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter Declaration of Conformity We herevrith infomn you that the Holmium Oxide Glass Filter Type Hoya HY 1 Part No 79830 22711 meets the following specification of absorbance maxima positions nae a i a Nunber Wavdength Acura Bandwidth f Tram Tron i emoa jooo reson Cd 79853C 1050 360 8ron i 2 ym 418 Som 536 4ron GI314AB C 1100 1200 360 8ron i l rm orm 418 St
73. nce 157 UV Radiation 158 Sound Emission 159 Declaration of Conformity for HOX2 Filter 160 Agilent Technologies on Internet 162 ee Agilent Technologies 151 6 Appendix General Safety Information 152 General Safety Information General The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any inten
74. nce and Repair Detector Overview of VWD Maintenance The following pages describe maintenance simple repairs of the detector that can be carried out without opening the main cover Table 29 Simple Repairs Procedures Typical Frequency Notes Deuterium lamp If noise and or drift exceeds your application limits or A VWD test should be performed after exchange lamp does not ignite replacement Flow cell exchange If application requires a different flow cell type A VWD test should be performed after replacement Cleaning flow cell parts If leaking or if intensity drops due to contaminated flow A pressure tightness test should be cleaning or exchange cell windows done after repair Leak sensor drying If leak has occurred Check for leaks Leak handling system If broken or corroded Check for leaks replacement Exchanging a Lamp When If noise or drift exceeds application limits or lamp does not ignite Tools required Screwdriver POZI 1 PT3 Parts required Partnumber Description 1 G1314 60100 Deuterium lamp Preparations Turn the lamp OFF 116 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Detector WARNING 1 Press the release buttons and remove the lower front cover to have access to the lamp Injury by touching hot lamp arca If the detector has been in use the lamp may be hot If so wait five minutes for lamp to cool down 2 Unscrew the lamp cover and remove it 3 Unscrew d
75. ng head assembly head assembly away from the sampler Notice that the closed side of the metering 1120 Compact LC User Manual 109 4 Preventive Maintenance and Repair the metering head assembly 3 Remove the two fixing bolts from the base of 4 Disassemble the metering head assembly 5 Use a small screwdriver to carefully remove the seal Clean the chamber with lint free cloth Ensure all particular matter is removed 6 Install the new seal Press the seal firmly into position 110 1120 Compact LC User Manual Preventive Maintenance and Repair 4 7 Place the piston guide on top of the seal 8 Reassemble the metering head assembly Carefully insert the plunger into the base The closed side of the metering head must be on the same side as the lower one of the two capillary drillings 9 Install the fixing bolts Tighten the bolts securely 10 Install the metering head assembly in the autosampler Ensure the large hole in the metering head is facing downwards 1120 Compact LC User Manual 111 4 Preventive Maintenance and Repair Autosampler 11 Reinstall the capillaries Next Steps 12 On completion of this procedure Install the front cover 13 Select End in the Tools function in the LMD Software Change piston 112 1120 Compact LC User Manual Gripper Arm When Defective gripper arm Tools requ
76. nt shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met
77. ntrolled spindle system to drive its plungers The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume The stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended Using the Pump Hints for successful use of the Compact LC pump e When using salt solutions and organic solvents in the Agilent 1120 Compact LC Pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve port It is best to have the organic channel directly above the salt solution channel Regular flushing with water of all DCGV channels is recommended to remove all possible salt deposits in the valve ports e Before operating the pump flush the vacuum degasser optional with at least two volumes 3 ml especially when turned off for a certain length of time for example during the night and volatile solvent mixtures are used in the channels e Prevent blocking of solvent inlet filters never use the pump without solvent inlet filter Growth of algae should be avoided 1120 Compact LC User Manual 45 3 Introduction and Optimizing Performance Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by
78. oard while the needle in vial position is determined by counting the motor steps from the upper needle sensor position 1120 Compact LC User Manual 51 3 52 Introduction and Optimizing Performance Analytical head The analytical head is driven by the stepper motor connected to the drive shaft by a toothed belt The drive nut on the spindle converts the circular movement of the spindle to linear motion The drive nut pushes the sapphire plunger against the tension of the spring into the analytical head The base of the plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides the movement of the plunger in the analytical head The home position of the plunger is sensed by an infra red sensor on the sampling unit flex board while the sample volume is determined by counting the number of steps from the home position The backward movement of the plunger driven by the spring draws sample from the vial Table 17 Analytical Head Technical Data Standard 100 pl Number of steps 15000 Volume resolution 7 nl motor step Maximum stroke 100 pl Pressure limit 600 bar Plunger material Sapphire Injection Valve The two position 6 port injection valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the
79. ompact LC User Manual Parts for Maintenance and Repair 5 Parts for Detector Maintenance Standard Flow Cell Table 39 Standard Flow Cell Assembly Item Description Part Number Standard Flow Cell 10 mm 14 ul 40 bar G1314 60086 1 Cell screw kit quantity 2 G1314 65062 2 Conical spring kit quantity 10 79853 29100 3 Ring 1 PEEK kit quantity 2 G1314 65065 4 Gasket 1 small hole KAPTON quantity 10 G1314 65063 5 Window quartz kit quantity 2 79853 68742 6 Gasket 2 large hole KAPTON quantity 10 G1314 65064 7 Ring 2 PEEK kit quantity 2 G1314 65066 1120 Compact LC User Manual 141 5 142 Parts for Maintenance and Repair Parts for Detector Maintenance Cell screw 1 Conical springs Ring 1 PEEK B Gasket 1 small hole i a ZA Window Quartz f 3 Gasket 2 large hole a Ring 2 PEEK A 5 Noor woNn Figure 40 Standard Flow Cell Detector Lamp Variable detector lamp part number G1314 60100 1120 Compact LC User Manual Parts for Maintenance and Repair 5 Parts for Repair Degasser Unit Degasser Unit part number G4280 60070 Solvent Delivery System Bottle Head Assembly Table 40 Bottle Head Assembly Parts Item Description Part Number Complete assembly G1311 60003 1 Ferrules with lock ring 5063 6598 10x 2 Tube screw 5063 6599 10x 3 Wire marker No part number 4 Solvent tubing 5 m 5062 2483 5 Frit adapter pack of 4 5062 8517 6 Solvent inlet filt
80. ompensates the previous described loss of volume during the delivery stroke of the first plunger Optimize the compressibility compensation setting The compressibility compensation default setting is 46 x 10 bar for the pump This setting represents an average value Under normal conditions the default setting reduces the pressure pulsation to values below 1 of system pressure that will be sufficient for most applications and for all gradient analyses For applications using sensitive detectors the compressibility settings can be optimized by using the values for the various solvents If the solvent in use is not listed in the compressibility tables when using isocratic mixtures of solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings When using mixtures of solvents it is not possible to calculate the compressibility of the mixture by interpolating the compressibility values of the pure solvents used in that mixture or by applying any other calculation In these cases the following empirical procedure has to be applied to optimize your compressibility setting 1120 Compact LC User Manual 43 3 44 Introduction and Optimizing Performance 1 Start the pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Use degassed solvent only Check the tightness of the system with the pressure
81. on valve seal After using aqueous buffers or 5 minutes salt solutions When visibly scratched or 10 minutes when the valve performance shows indication of leakage or wear After approximately 10000 to 10 minutes 20000 injections or when the valve performance shows indication of leakage or wear Flushing the manual injector CAUTION The use of aqueous buffers or salt solutions can lead to crystal formation Crystal formation may cause scratches on the injection seal gt Always rinse the valve with water after using aqueous buffers or salt solutions 1 Switch the valve to the INJECT position 2 Use the pump to flush the sample loop and seal grooves 3 Use the needle port cleaner supplied with the valve and syringe to flush the needle port and vent capillary 90 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Needle port Syringe SP Ch Needle port cleaner Figure 31 Needle port cleaner 1120 Compact LC User Manual 91 4 When Tools required Preventive Maintenance and Repair Injection Valve Seal Poor injection volume reproducibility Leaking injection valve Hex key 9 64 inch 8710 2394 Parts required Partnumber Description 1 5067 4105 Rotor seal Vespel Loosen the three stator screws Remove the stator head 2 Remove the stator ring Ys fe y A y A Wy 9 y l 2 LZ ve SAI S T ET
82. otor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first plunger to move at twice the speed of the second plunger The solvent enters the pump head close to the bottom limit and leaves it at its top The outer diameter of the plunger is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between The first plunger has a stroke volume in the range of 20 100 ul depending on the flow rate The microprocessor controls all flow rates in a range of 1 ul 10 ml min The inlet of the first plunger chamber unit is connected to the inlet valve which is opened or closed allowing solvent to be drawn into the first plunger pump unit The outlet of the first plunger chamber unit is connected through the outlet ball valve and the damping unit to the inlet of the second plunger chamber unit The outlet of the purge valve assembly is then connected to the following chromatographic system 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Chamber 1 Chamber 2 Purge valve p To column Inlet valve To waste From solvent bottle Seal Plunger 1 Plunger 2 Ball screw drive Gear Motor with encoder Figure 15 Principle of the pump When turned on the pump runs through an initialization procedure to determine the upper dead center of the first plunger The first plunger moves slo
83. place the front cover Using the Cuvette Holder This cuvette holder can be placed instead of a flow cell in the variable wavelength detector Standard cuvettes with standards in it for example National Institute of Standards amp Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications When If your own standard should be used to checkout the instrument Tools required None Parts required Partnumber Description 1 G1314 60200 Cuvette Holder 1 Cuvette with the standard e g NIST certified holmium oxide sample Preparations Remove the normal flow cell Have cuvette with standard available 120 1120 Compact LC User Manual Preventive Maintenance and Repair 4 1 Locate the cuvette holder on the desk 3 Insert the cuvette with the sample into the holder The clear side of the cuvette must be visible B Light path Clear side 2 Unscrew the bracket 4 Reset the lamp counter as described in the User Interface documentation 5 Turn the lamp ON Give the lamp more than 10 minutes to warm up 7 Perform Wavelength Verification Calibration to check the correct positioning of the lamp 1120 Compact LC User Manual 121 4 Preventive Maintenance and Repair 8 Replace the bracket and fix the cuvette Next Steps 9 Install the cuvette holder in the instrument 10 Perform your verification 122 1120 Comp
84. pler sample trays 56 Internet 162 introduction detector 61 164 L Lab Monitoring and Diagnostic Software 67 lamp intensity test 124 type 13 LAN Bootp amp Store 23 Bootp service 32 Bootp 23 configuration switches 22 configuration with Bootp 32 initialization mode selection 23 link configuration selection 26 manual configuration with telnet 28 storing the settings permanently 27 TCP IP parameter configuration 22 using default 25 using stored 24 what you have to do first 20 leak correcting 123 linearity 14 13 line power consumption 10 voltage and frequency 10 link configuration selection 26 liquimeter 68 LMD 11 67 LOAD 55 M MAC address 20 mainpass 49 maintenance functions 96 maintenance correcting leaks 123 exchanging flow cell 119 exchanging lamps 116 overview 116 using the cuvette holder 120 manual configuration of LAN 28 materials in contact with mobile phase 42 metering device 51 metering seal 138 multi draw option 48 needle drive 51 51 needle port cleaner 90 noise short term 13 numbering of vials 56 0 operating temperature 10 operational pressure range 12 optical unit parts 150 outlet ball valve assembly parts 133 outlet capillary 144 P parts and materials analytical head assembly 138 autosampler main assemblies 138 autosampler sampling unit assembly 146 autosampler thermostat 146 injection valve assembly 147 main assemblies 138 transport assembly 146 parts bottl
85. r 160 Agilent Technologies on Internet 162 1120 Compact LC User Manual 1120 Compact LC User Manual e 1 General Information about the Agilent 1120 Compact LC System System Configurations 6 Site Requirements 7 Power Consideration 7 Power Cords 8 Bench Space 8 Environment 9 Physical Specification 10 Performance Specifications 11 This chapter provides an overview to the Agilent 1120 Compact LC Configura tions Site Requirements and Specifications aa Agilent Technologies 5 1 General Information about the Agilent 1120 Compact LC System System Configurations Available configurations of Agilent 1120 Compact LC Systems The Agilent 1120 Compact LC System is provided in 5 different configurations possible components are isocratic pump dual channel gradient pump with degasser manual injector autosampler column oven and detector EAch configuration comes at least with one pump one injection system and one detector per system including EZ Chrom Elite Compact Software and Lab Monitoring and Diagnostic Software Isocratic pump Isocratic pump Gradient pump Gradient pump Gradient pump Manual injector Autosampler Manual injector Manual injector Autosampler Column plane Column oven Column plane Column oven Column oven Variable Variable Variable wavelegth wavelegth wavelegth detector detector detector Variable wavelegth detector Variable wavelegth detector G4286 64010
86. rate of 5 ml min of H30 with purge valve opened Purge valve if internally leaking Wrench 1 4 inch Wrench 14 mm Pair of tweezers or toothpick Partnumber Description 5 01018 22707 PTFE frit pack of 5 1 G4280 60031 Purge valve Switch off pump at the main power switch Remove the upper front cover 1 Using a 1 4 inch wrench disconnect the pump outlet capillary at the purge valve 2 Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure 3 Using the 14 mm wrench unscrew the purge valve and remove it 4 Remove the plastic cap with the gold seal from the purge valve 5 Using a pair of tweezers or a toothpick remove the frit Valve body 2 id PTFE frit Gold seal Ma Plastic cap Figure 29 Purge Valve Parts 1120 Compact LC User Manual 77 4 Preventive Maintenance and Repair 6 Place a new frit into the purge valve with the orientation of the frit as shown above 7 Reinstall the cap with the gold seal Before reinstallation always check the gold seal A deformed seal should be exchanged 8 Insert the purge valve into the pump head and locate the pump outlet capillary and the waste tube 9 Tighten the purge valve and reconnect outlet capillary and waste tubing Outlet capillary Purge valve Waste tube Figure 30 Exchanging the Purge Valve 78 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Removing the Pump Head Assembly When Befo
87. re exchanging the seals Before exchanging the plungers Tools required Wrench 1 4 inch 4 mm hexagonal key 8710 2392 Preparations Switch off pump at the main power switch WARNING Starting the pump when the pump head is removed may damage the pump drive Damage of the pump drive gt Never start the pump when the pump head is removed Remove the upper front cover Using a 1 4 inch wrench remove the outlet capillary Disconnect the capillary from the outlet ball valve Remove the waste tubing and disconnect the inlet valve tubing Remove the capillary at the bottom of the pumphead O 0O A Q N Using a 4 mm hexagonal key stepwise loosen the two pumphead screws and remove the pumphead from the pump drive 1120 Compact LC User Manual 79 4 Preventive Maintenance and Repair Solvent Delivery System Pump head screws 80 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Exchanging the pump seals and seal wear in procedure When Seal leaking if indicated by the results of the leak test Tools required Wrench 1 4 inch 4 mm hexagonal key Parts required Partnumber Description 2 5063 6589 or standard Seals pack of 2 or 0905 1420 seals for normal phase applications 1 5022 2159 Restriction capillary Preparations Switch off pump at the main power switch Remove the upper front cover 1 Place the pump head on a flat surface 2 Remove the support rings from the plunger Loosen the lock s
88. s are allowing it Non Volatile RAM Stored Parameter Figure 7 Manual Configuration Principle With Telnet Whenever a TCP IP connection to the Compact LC is possible TCP IP parameters set by any method the parameters may be altered by opening a Telnet session 1 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and press OK 2 Type the following at the system DOS prompt c gt telnet lt IP address gt or 1120 Compact LC User Manual Installing the Agilent 1120 CompactLC 2 c gt telnet lt host name gt E C WINDOWS system32 cmd exe C gt telnet 134 40 27 95 Figure 8 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle or the default IP address When the connection was established successfully the Compact LC responds with the following ct Telnet bo xx xx xx Agilent Technologies G4296A DEGHHHHHHH gt Figure 9 A connection to the module is made 3 Type and press enter to see the available commands ex Telnet 1xx xx xx xx Agilent Technologies G4296A DEHOOOHOHA gt command syntax description display help info display current LAN settings set IP Address set Subnet Mask set Default Gateway exit shell Figure 10 Telnet Commands 1120 Compact LC User Manual 29 2 30 Installing the Agilent 1120 Compact LC Table 14 Telnet Commands V
89. s with longer path lengths yield higher signals Although noise usually increases little with increasing path length there is a gain in signal to noise ratio For example the noise increased by less than 10 but a 70 increase in signal intensity was achieved by increasing the path length from 6 mm to 10 mm When increasing the path length the cell volume usually increases in our example from 5 13 ul Typically this causes more peak dispersion As Figure 25 on page 63 demonstrates this did not affect the resolution in the gradient separation in our example As a rule of thumb the flow cell volume should be about 1 3 of the peak volume at half height To determine the volume of your peaks take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3 Analysis of pesticide standard 6 mm optical path length 10 mm optical path length Absorbance i i j ty Time min Figure 25 Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards To check photometric accuracy of the Agilent 1120 Compact LC VWD it is necessary to have more precise information on path lengths of the VWD flow cells The correct response is expected response correction factor Please find below the details of the Agilent 1120 Compact LC VWD flow cells 1120 Compact LC User Man
90. struments Picture shows a typical intensity test spectrum Intensity Test Evaluation The Agilent LMD Software evaluates three values automatically and displays the limits for each value the average the minimum and the maximum of all data points and passed or failed for each value Test Failed e Absorbing solvent in flow cell e Dirty or contaminated flow cell e Dirty or contaminated optical components source lens mirrors grating e Old or non Agilent lamp Y Ensure the flow cell is filled with water V Repeat the test with the flow cell removed If the test passes exchange the flow cell windows Y Clean replace optical components V Exchange the lamp 1120 Compact LC User Manual Preventive Maintenance and Repair 4 30000 i 4 Highest intensity gt 320000 cts 7123680 cts Passed Average intensity gt 160000 cts 951488 cts Passed Lowest intensity gt 6400 cts 36384 cts i Wavelength Verification Calibration Wavelength calibration of the detector is done using the zero order position and 656 nm alpha emission line and beta emission line at 486 nm emission line positions of the deuterium lamp The calibration procedure involves three steps First the grating is calibrated on the zero order position The stepper motor step position where the zero order maximum is detected is stored in the detector Next the grating is calibrated against the deuterium emission line at 656 nm and the motor position a
91. t for verification Pressure ripple unstable run leak test for verification Solvent dripping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit 5 ml min H30 with purge open indicates blockage Leaks at lower pump head side unstable retention times pressure ripple unstable run leak test for verification Run leak test for verification Seal life time shorter than normally expected check plungers while changing the seals 70 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Instrument is partially energized when switched off The power supply still uses some power even if the switch on the front panel is turned off gt To disconnect the Compact LC pump from line unplug the power cord Sharp metal edges Sharp edged parts of the equipment may cause injury gt To prevent personal injury be careful when getting in contact with sharp metal areas When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD
92. t which the maximum occurs is stored in the detector Finally the grating is clabrated against the deuterium emission line at 486 nm and the motor position at which the maximum occurs is stored in the detector 1120 Compact LC User Manual 125 4 Preventive Maintenance and Repair The wavelength verification calibration takes about 2 5 minutes and is disabled within the first 10 minutes after ignition of the lamp because initial drift may distort the measurement When the lamp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically Holmium Oxide Test This test verifies the calibration of the detector against the three wavelength maxima of the built in holmium oxide filter The test displays the difference between the expected and measured maxima Picture shows a holmium test spectrum The test uses the following holmium maxima e 360 8 nm e 418 5 nm e 536 4 nm When to do the Test e after recalibration e as part of the Operational Qualification Performance Verification procedure or e after flow cell maintenance or repair Interpreting the Results The test is passed successfully when all three wavelengths are within 1 nm of the expected value This indicates the detector is calibrated correctly 126 1120 Compact LC User Manual Preventive Maintenance and Repair 4 Absorbance AU 200 200 400 501 600 Wavelangth nm Holmium Oxide Test Failed Probable Caus
93. taneously Numbering of vial positions The standard 100 vial tray has vial positions 1 to 100 However when using two half trays the numbering convention is slightly different The vial positions of the right hand half tray begin at position 101 as follows Left hand 40 position tray 1 40 Left hand 15 position tray 1 15 Right hand 40 position tray 101 140 Right hand 15 position tray 101 115 Choice of Vials and Caps For reliable operation vials used with the Agilent 1120 Compact LC autosampler must not have tapered shoulders or caps that are wider than the body of the vial The vials and caps in shown in below table shown with their Part numbers have been successfully tested using a minimum of 15 000 injections with the Agilent 1120 Compact LC autosampler Table 20 Crimp Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5181 3375 5183 4491 Clear glass 2 5182 0543 5183 4492 5183 4494 write on spot Amber glass 2 5182 3376 5183 4493 5183 4495 write on spot Polypropylene 1 5182 0567 5183 4496 wide opening Polypropylene 0 3 9301 0978 wide opening 1120 Compact LC User Manual 57 3 58 Introduction and Optimizing Performance Table 21 Snap Top Vials continued Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0544 5183 4504 5183 4507 Clear glass 2 5182 0546 5183 4505 5183 4508 write on spot Amber
94. ted to specific user requirements The wear of autosampler components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument The autosampler provides two EMF counters Each counter increments with autosampler use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The autosampler provides the following EMF counters Injection valve counter This counter display the total number of switches of the injection valve since the last reset of the counter Needle movements counter This counter displays the total number of movements of the needle into the seat since the last reset of the counter EMF counter for the variable wavelength detector The user settable EMF limits for the EMF counter enables the early maintenance feedback to be adapted to specific user requirements The useful lamp burn time is dependent on the requirements for the analysis high or low sensitivity analysis wavelength and so on therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument The detector module provides a EMF counter for the lamp The counter increments with lamp use and can be assigned a maximum limit which provides visual feedback in
95. tem without solvent filter installed 46 1120 Compact LC User Manual Introduction and Optimizing Performance 3 Injection System This chapter provides an overview on the operational principles of the Injec tion Systems Manual Injector and Autosampler Introduction to the Manual Injector The Agilent 1120 Compact LC manual injector uses a Rheodyne 6 port sample injection valve part number 5067 4102 Sample is loaded into the external 20 ul sample loop through the injection port at the front of the valve The valve has a PEEK injection seal A make before break passage in the stator ensures flow is not interrupted when the valve is switched between the INJECT and LOAD positions and back again Load Inject Figure 16 Rheodyne 6 port sample injection valve 1120 Compact LC User Manual 47 3 48 Introduction and Optimizing Performance Introduction to the Autosampler Introduction to the Autosampler Three sample rack sizes are available for the autosampler The standard full size rack holds 100 x 1 8 ml vials while the two half size racks provide space for 40 x 1 8 ml vials and 15 x 6 ml vials respectively Any two half size rack trays can be installed in the autosampler simultaneously The analytical head device provides injection volumes from 0 1 100 ul The autosamplers transport mechanism uses an X Z Theta movement to optimize vial pick up and return Vials are picked up by the gripper arm and posi
96. tioned below the sampling unit The gripper transport mechanism and sampling unit are driven by motors Movement is monitored by optical sensors and optical encoders to ensure correct operation The metering device is always flushed after injection to ensure minimum carry over The six port injection valve unit only 5 ports are used is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosampler and directly connects the flow from the pump to the column During injection and analysis the valve unit directs the flow through the autosamplers which ensures that the sample is injected completely into the column and that any sample residue is removed from the metering unit and needle from before the next sampling sequence begins Sampling Sequence The movements of the autosampler components during the sampling sequence are monitored continuously by the processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence can t be completed successfully an error message is generated Solvent is bypassed from the autosamplers by the injection valve during the sampling sequence The sample vial is selected by a gripper arm from a static sample rack The gripper arm places the sample vial below the injection needle The required volume of sample is drawn into the sample loop by the metering device Sample is applied to the col
97. tnumber Description 1 G4280 60005 Passive Inlet Valve Place the solvent bottles under the pump 1 Remove the upper front cover 2 Disconnect the solvent inlet tube at the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow 3 Unscrew the adapter from the inlet valve optional 4 Using a 14 mm wrench loosen the inlet valve and remove the valve from pump head Figure 26 Passive Inlet Valve Parts 4 Passive Inlet Valve part number G4280 60005 1 O Ring part number 0905 1684 2 Gold Seal part number 5001 3707 3 Cap pack of 4 part number 5062 2485 1120 Compact LC User Manual 73 4 Preventive Maintenance and Repair 5 Check that the new valve is assembled correctly and that the gold seal is present if the gold seal is deformed it should be replaced 6 Insert the new valve into the pump head 7 Using the 14 mm wrench turn the nut until it is hand tight 8 Reconnect the adapter at the inlet valve optional 9 Reconnect the solvent inlet tube to the adapter 10 Reinstall the front cover After an exchange of the valve it may take several ml of pumping with the solvent used in the current application before the flow stabilizes at a ripple as low as it used to be when the system was still working properly Exchanging the Outlet Ball Valve When Tools required Parts required Preparations 74 If internally leaking Wrench 1 4 inch Partnumber Description
98. ual 63 3 Introduction and Optimizing Performance Table 26 Correction factors for Agilent VWD flow cells Flow cell type Cell Part number Pathlength Path length Correction volume nominal actual factor Standard flow cell 14 ul G1314 60086 10mm 10 15 0 19 mm 10 10 15 Semi micro flow cell 5 ul G1314 60083 6mm 6 10 0 19 mm 6 6 10 Micro flow cell 2 ul G1314 60087 3mm 2 80 0 19 mm 3 2 8 High Pressure flow cell 14 ul G1314 60082 10 mm 10 00 0 19 mm 6 5 75 64 Be aware that there are additional tolerance of gasket thickness and its compression ratio which is supposed to be very small in comparison with the machining tolerance 1120 Compact LC User Manual 1120 Compact LC User Manual e 4 Preventive Maintenance and Repair Preventive Maintenance Scope of Work and Checklist 67 Early Maintenance Feedback EMF 68 EMF counters forthe pump 68 EMF Counters for the autosampler 69 EMF counter for the variable wavelength detector 69 Solvent Delivery System 70 Introduction and Overview to simple repairs of the pump 70 Checking and Cleaning the Solvent Filter 72 Exchanging the Passive Inlet Valve 73 Exchanging the Outlet Ball Valve 74 Exchanging the Purge Valve Frit or the Purge Valve 77 Removing the Pump Head Assembly 79 Exchanging the pump seals and seal wear in procedure 81 Exchanging the Plungers 84 Reinstalling the Pump Head Assembly 86 Exchanging the Dual Channel Gradient Valve DCGV 87 Manual Injector 90 Overv
99. um is not used for degassing most algae need oxygen and light for growth The presence of algae in the Agilent 1120 Compact LC can cause the following to occur 1120 Compact LC User Manual Introduction and Optimizing Performance 3 e PTFE frits part number 01018 22707 purge valve assembly and column filter blockage causing increased system pressure Algae appear as white or yellowish white deposits on filters Typically black particles from the normal wear of the piston seals do not cause the PTFE frits to block over short term usage Short lifetime of solvent filters bottle head assembly A blocked solvent filter in the bottle especially when only partly blocked is more difficult to identify and may show up as gradient performance problems intermittent pressure fluctuations etc Algae growth may also be the possible source for failures of the ball valves and other components in the flow path How to Prevent and or Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle part number 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase Compact LC electronics All el
100. umn when the injection valve returns to the mainpass position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The injection valve switches to the bypass position 1120 Compact LC User Manual Introduction and Optimizing Performance 3 2 The plunger of the metering device moves to the initialization position wow The gripper arm selects the vial At the same time the needle lifts out of the seat The gripper arm places the vial below the needle The needle lowers into the vial The metering device draws the defined sample volume The needle lifts out of the vial ono A If the automated needle wash is selected the gripper arm replaces the sample vial positions the wash vial below the needle lowers the needle into the vial then lifts the needle out of the wash vial 9 The gripper arm checks if the safety flap is in position 10 The gripper arm replaces the vial Simultaneously the needle lowers into the seat 11 The injection valve switches to the mainpass position Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position In this position the mobile phase flows through the autosamplers metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over Figure 17 Mainpass Position 1120 Compact LC User Manual 49
101. wly upwards into the mechanical stop of chamber and from there it moves back a predetermined path length The controller stores this plunger position in memory After this initialization the pump starts operation with the set 1120 Compact LC User Manual 41 3 Introduction and Optimizing Performance Table 15 parameters The inlet valve is opened and the down moving plunger draws solvent into the first chamber At the same time the second plunger is moving upwards delivering into the system After a controller defined stroke length depending on the flow rate the drive motor is stopped and the inlet valve is closed The motor direction is reversed and moves the first plunger up until it reaches the stored upper limit and at the same time moving the second plunger downwards Then the sequence starts again moving the plungers up and down between the two limits During the up movement of the first plunger the solvent in the chamber is pressed through the outlet ball valve into the second chamber The second plunger draws in half of the volume displaced by the first plunger and the remaining half volume is directly delivered into the system During the drawing stroke of the first plunger the second plunger delivers the drawn volume into the system For solvent compositions from the solvent bottles A B the controller divides the length of the intake stroke in certain fractions in which the gradient valve connects the specified solvent channel to th

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