Leica M125, M165 C, M205 C, M205 A Manual
Contents
1. Trinocular video phototube 50 Use With its third beam path the trinocular video phototube 50 enables you to simultaneously view and photograph a specimen The available light is divided as follows X 50 is available for the two eyepieces 50 of the light is diverted to the video photo beam path Assembly Fasten the trinocular tube 50 to the optics carrier instead of the binocular observation tube refer to page 22 Leica M series Manual 66 Trinocular video phototube 100 Use Switchover With its third beam path the trinocular video Turn the controller on the right side of the phototube 100 enables you to either view or tube into the horizontal position in order to photograph a specimen This means that 100 guide all available light into the eyepieces of the light is available to one or the other beam You can now observe the specimen path The other beam path remains opaque or black Turn the controller on the right side of the tube into the vertical position in order to Assembly guide all available light into the camera Fasten the trinocular tube 100 to the optics You can now photograph the specimen carrier instead of the binocular observation tube refer to page 22 Leica M series Manual 67 Microscope Carrier The Microscope Carrier AX Users who intend to do a great deal of photog raphy take measurements or work with polar ization should select the m2. Footswitch 1 Footswitch Za Connect to free CTL2 port on last footswitch Connector from Cross Stage Leica M series Manual 42 Leica LED5000 MCI The Leica LED5000 MCI for Multi Contrast Illumination is installed using two screws For optimum accessibility the optics carrier should be removed during installation see page 15 Constraints The Leica LED5000 MCI cannot be used together with the objective nosepiece Leica M series Assembly 1 Hold the LED5000 MCI with one hand and tightly screw the retraining stirrups on both top holes on the drive hous ing Manual Connect the CAN bus cable to either of the two sockets The flat part of the plug must be facing downwards Plug the other end of the cable into one of the two CTL2 sockets on the column 43 Leica LED5000 MCI Alternative Assembly Under certain circumstances the light source must not be moved along with the optics carrier A typical example is multifocus images in which the Z stack changes while the angle of incidence of the light must remain the same For such purposes the Leica LED5000 MCI is directly fastened to the column Installation on the column 1 Pull the retaining stirrup out of the Leica LED5000 MCI Leica M series 2 Screwthe retaining stirrup into the column at the notch using the single screw If you tighten the screw the retaining stirrup is automatically moved into the corr
3. diopter settings until the image is uniformly sharp Manual Note that when using dioptric correction the advantage of parfocality is lost thus you have to manually refocus each time you change the zoom level To also use parfocality with dioptric correction refer to the instruc tions on page 61 60 Dioptric Correction and Parfocality Leica stereomicroscopesare parfocally matched The prerequisite for this is the correct setting of the diopters and the parfocality The following adjustments only have to be carried out once by each user Preparations Move the lever of the video phototube to the observation position and open the diaphragm If you are using the microscope carrier AX set it to stereoscopic observation Leica M series Adjusting 1 Set the dioptric correction for both eyepieces to 0 Select the lowest magnification and focus ona flat specimen Select the highest magnification and read just the sharpness Select the lowest magnification again but do not look into the eyepieces Rotate the eyepieces counterclockwise in the direction as far as they will go 5 diopter settings Manual 7 Look into the eyepieces 8 Slowly rotate each eyepiece individually in the direction until each eye sees the object sharply imaged 9 Select the highest magnification and refo cus if necessary Now if you adjust the magnification from the lowest to the highes
4. In dark field illumination a ring illuminator is used in such a way that the direct light does not reach the objective without a speci men Only the structure of semitransparent opaque objects disperses the light making the object visible against a dark background Turn the control as far as it will go towards DF dark field Identical subject with dark field illumination 79 TLRC TL RCI Controls Vertical column transmitted light base adapter plate 1 Power switch l l o 2 Power connection socket 1 di sink of the Degen halogen illumina 3 USB socket type B 208 ony SM l l 4 USB socket type A Extension plate for focusing drives 5 2x CAN bus Standard stage 10447 269 6 Screws for changing the halogen lamp Filter holder Control of top and bottom flaps of the Rottermann Contrast 6 Button for mirror and horizontal movement of the mirror 7 Transmitted lightbase Wm A LUN Leica M series Manual 80 TL RCI The Deflection Mirror Inverted world Depending on the properties of the specimen refractive index of the environment and the perception of the viewer it is possible that the switches described below for positive and inverted relief contrast are to be operated in reverse This means that the lower switch rather than of the upper switch controls inverted relief contrast and vice versa Leica M series Tilted mirror The built in mirror features one flat and one concave
5. Leica M125 Leica M165 C Leica M205 C Leica M205 A Manual MICROSYSTEMS General Instructions Safety concept Before using your microscope for the first time please read the Safety concept brochure included with your instrument It contains addi tional information about handling and care Bezpe nostn koncepce Sikkerhedskoncept Sicherheitskonzept Ohutuseeskiri Safety concept Concepto de seguridad Concept de s curit Concetto di sicurezza Dro bas noteikumi Saugos samprata Biztons gi el r sok Veiligheidsconcept Sikkerhetskonsept Koncepcja bezpiecze stwa Conceito de seguran a Bezpe nostn pokyny Varnostni koncept Turvallisuus S kerhetskoncept LEME MICROSYSTEMS FICHI SERE Leica M series Use in clean rooms The Leica M series can be used in clean rooms without any problems Cleaning Do not use any unsuitable cleaning agents chemicals or techniques for cleaning Xx Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abraded particles In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms Manual Servicing Repairs may only be carried out by Leica Microsystems trained service technicians Only original Leica Microsystems spare parts may be used Respons
6. CCIC configured settings Constant Color Intensity Control illumina tion intensity 2 Potentiometer for controlling the IsoCol color temperature Leica M series Manual Using a USB mouse only TL RCI The Leica USB mouse controls the CCIC and dimming function of the TL RCI base Connect the mouse to the corresponding USB port of the base The scroll wheel of the mouse is occupied by default with the CCIC control system and is used to control the illumination intensity To switch the illumination on or off briefly click the scroll wheel To enter or exit dimming mode click and hold down the scroll wheel for longer than 2 seconds 82 TL RC TL RCI Operation Setting the relief contrast The two switches on the left side of the trans mitted light base TL RC TL RCI activate two built in flaps The outer switch 1 controls the inverted relief contrast and the inner switch 2 adjusts the positive relief contrast 1 Switch for adjusting the inverted relief contrast 2 Switch for adjusting the positive relief contrast 3 Deflection mirror Leica M series Depending on the flap position a part of the opening of the built in Fresnel lenses are covered which results in the different contrast effects Phase structures typically act as spatial relief type images in the positive relief contrast like hills in the inverted relief contrast like valleys Manual Incr
7. Leica LED5000 RL Leica M series Manual 105 Specifications for the Bases Transmitted light Base TL ST Light source Halogen lamp 12V 20W Quick illuminant change Yes Illuminated area 50mm Power supply unit Input voltage 100 240 V frequency 50 60 Hz energy consumption 30 W max ambient temperature 10 40 C Connections Power plug power switch Weight 7 4kg Illumination types Bright field Yes Dark field Yes single sided Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control No Integrated filter holder Yes Coated optics for increasing the color Yes temperature Matching of high num aperture No Remote control options No AntiShock Pads Yes Dimensions WxHxD 340x430x85 mm Leica M series Manual 107 Transmitted light Base TL BFDF Light source External via cold light source Illuminated area 40mm Connections Connection for cold light guide active f 10mm end tube f 13mm Weight 5 8 kg Illumination types Bright field Yes Dark field Yes Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control Yes Integrated filter holder No Coated optics for increas
8. M series like its predecessors remains compatible with existing series This means that objectives bases tubes and so on can be reused Objectives All new objectives of the M series are parfocal meaning that when used with the objective nosepiece they can be replaced while keeping the specimen in perfect focus If you prefer you can continue to use the previ ous objective series In this case parfocality is no longer guaranteed Leica M series Tubes The interface between the optics carrier and the tube has remained the same so existing tubes fit the new M series The new tubes are designed for eyepieces with field number 23 while the predecessor models were only designed for field number 21 resulting in a smaller object field Eyepieces However the new M series eyepieces models have an audible and tangible click to provide immediate feedback in case of accidental adjustment Manual On We Go If your new Leica microscope has already been If on the other hand you are assembling the assembled and commissioned by your Leica microscope yourself continue with the Assem consultant click here to skip through the instal bly chapter which begins on page 19 lation instructions and go directly to the Quick Start Guide on page 46 Leica M series Manual 18 Assembly Base and Focusing Column The first step is to connect the focusing column Assembling the column adapter Assembling the focus
9. Safety 15 Leica LED5000 MCI 43 The Modular Design Everything is Relative 16 Leica LED5000 MCI Alternative Assembly 44 Maximum Compatibility 17 Leica LED5000 RL 45 On We Go 18 Base and Focusing Column 20 The Fastest Route to Success 47 Optics carrier 21 Overview of an M series Microscope 48 Tube 22 The Correct Interpupillary Distance 49 Eyepieces 23 Using the Eyepieces 50 Objective 24 Focusing 51 Objective Nosepiece Assembly 25 Adjusting the resistance of the focus drive 52 Objective Nosepiece Adjusting Parfocality 26 Changing the magnification zoom 53 AX Carrier Preparation 27 Ratchet Steps and Magnification Levels 54 AX Carrier Assembly 28 Parfocality More Comfort and Convenience for Your Work 55 Transmitted light Base TL ST 29 Iris Diaphragm 56 Leica M series Manual 9 Bases Magnification Factors of the Eyepieces 58 Transmitted light Base TL ST Controls 75 Health Notes 59 Transmitted light Base TL ST Operation 76 Dioptric correction 60 Transmitted light Base TL ST Lamp Replacement 77 Dioptric Correction and Parfocality 61 Transmitted light Base TL BFDF Controls 78 Graticules 62 Transmitted light Base TL BFDF Operation 79 TL RC TL RCI Controls 80 TLRCI The Deflection Mirror 81 Photography amp Video 64 TL RCI Color Intensity and Temperature 82 Photo tubes and C mounts 65 TL RC TL RCI Operation 8
10. appears sharp Toggle to the 2x objective Select the strongest magnification and refocus until the specimen appears abso lutely sharp Manual 7 Toggle to the 1x objective 8 Turn the objective on the thread in both directions until the specimen appears absolutely sharp By means of zooming check that the behavior of the objective is parfocal Repeat the check with the other objective If it is not parfocal repeat the procedure 9 Tighten the locking screws 26 AX Carrier Preparation Tools If the switch position corresponds to the Hex socket screwdriver 2 5mm optics carrier used skip the following steps Otherwise proceed as follows Preparing the AX carrier 2 Unscrew the two screws that hold the Before the AX carrier is installed it may first Switch in position have to be prepared for the optics carrier to be used Leica M125 M205 C or M165 C 4 Important push the switch all the way into the mount while reinserting the screws Only this ensures that the switch is posi tioned correctly 1 Check the switch position 3 Pull out the switch rotate it into the desired position and reinsert it Leica M series Manual 27 AX Carrier Assembly Tools Hex socket screwdriver 4mm Philips screwdriver Once the AX carrier has prepared for the corre sponding optics carrier it can be installed 1 Move the slide of the AX carrier into mid position Wrong Righ
11. described in this operating manual have been tested for safety and potential hazards The responsible Leica affiliate must be consulted whenever the device is altered modified or used in conjunction with Leica M series non Leica components that are outside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Location of use See Safety concept booklet Xx Setup the electrical components at least 10 cm from walls and combustible objects Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort measurements and micro graphic images In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Manual Requirements for the owner operator See Safety concept booklet Ensure that Xx The M series stereomicroscopes and accessories are operated maintained and repaired by authorized and trained person nel only All operators have read understood and observe this user manual and particularly the safety instructions Safety regulations continued Repair service work Installation in third party products See Safety concept booklet See Safety concept booklet Only original Leica Microsystems spare parts may be used Disposal See Safety concept booklet Before opening the ins
12. eyepieces Leica M series Limits are made to be broken The Leica M205 C is the world s first stereo microscope with a zoom range of 20 5 1 This accomplishment however was not enough for Leica s engineers With the new FusionOptic in the M205 C they have succeeded in going yet another step beyond previous limits In addition to the increase in magnification the resolution too has been increased to up to 1050 Ip mm which corresponds to a resolved structure size of 472 nm Of course this performance increase benefits your everyday work Set up your specimens on the microscope with comfortable freedom of movement and discover details in stereomi croscopy that you could never see before Manual The Electronics Comfort Convenience and Safety for your Experiments Never before have electronics been used as extensively in a Leica series as in the new M series Optics carrier tripod base and illu minator are all connected using electrical contacts which provides a number of advan tages Contacts not only transmit data but also supply the power Leica M series Reliability for your experiments Exact reproducibility of results is an increas ingly common demand particularly in research and development The continuous encoding captures parameters such as the magnifica tion the illumination the position of the iris Leica Application Suite LAS evaluates the transmitted data and can restore the test
13. one benefit of viewing with eyeglasses is a drastically lower risk of bacte rial transmission see page 59 The soft mate rial of the eyecup also ensures that your glasses will not be scratched even if they contact the eyepiece 50 Focusing Focusing raises or lowers the stereomicroscope Focusing Coarse fine adjustment using the focusing drive The specimen detail is The inner coarse adjustment is used for The sharpness is adjusted using the coarse fine brought into sharp focus as soon as it is in the covering great distances adjustment focal point of the objective The outer fine adjustment is used for fine The coarse fine adjustment carries a The focusing drive can be operated either focusing load of up to 15 kg left or right handed X The resolution of the coarse fine adjust ment is 1 um Leica M series Manual 51 Adjusting the resistance of the focus drive Adjusting the resistance Is the focus movement too loose or too tight Does the outfit tend to slide downwards The resistance can be adjusted individually depend ing on the equipment weight and personal preferences as follows 1 Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focus ing Leica M series Manual 52 Changing the magnification zoom All M series microscopes have an integrated Zooming zoom The name indicates the zoom range 1 Look into the ey
14. risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Leica M series Light sources safety instructions Protective measures of the manufacturer UV protection screen in front of the speci men plane prevents the user from looking directly into the UV rays Dummy filter carriers in the free positions of the rapid filter changer prevent direct UV radiation from reaching the eyes UV filters are installed in the observation beam paths to protect the eyes The stray light protection on the lamp housing prevents irradiation of the hands Manual Warning UV radiation could damage the eyes Therefore Never look into the light spot on the speci men plane without a UV protection screen Never look into the eyepieces if no excita tion filter is in the beam path Fill empty filter positions with dummy filter carriers e g M165FC Do not select a white strongly reflective background for the stage Safety regulations continued Supply unit Always unplug the supply unit from the power supply When installing and disassembling the lamp housing Before opening the lamp housing When replacing the high pressure mercury lamp and other parts such as the heat ab sorbing filter or the collector during maintenance work on the supply unit Leica M series Lamp housing Never open the lamp housing while the lamp is switched on Risk of expl
15. situa tion later Manual diaphragm and more to the Leica LAS software Thus you always know the conditions under which an image was acquired Fewer cables A large part of the cables have been routed in the interior of the column Data are also trans ferred via the interface between the column the optics carrier and the carrier The direct result is that you need fewer cables this not only makes your workstation neater and more comfortable it even makes it appear larger The Modular Design Everything is Relative The Leica M series provides maximum flexibil ity in choosing equipment thanks primarily to the modular configuration and the compatibil ity that Leica has painstakingly maintained for decades The optics carriers eyepieces bases and more can be combined in any way you choose allowing you to create the microscope that best suits your needs Leica M series Despite this you will notice that the controls and individual components do not differ signif icantly Whichever configuration you choose you will quickly feel right at home Manual Have a special request Let us know Leica Microsystems enjoys an exceptional repu tation when it comes to devising customer specific solutions If you have a special request that cannot be met with standard parts contact your Leica consultant We have a solution for every problem Maximum Compatibility Leica engineers were careful to ensure that the new
16. the mount and reinsert the lamp holder Manual Precautionary measures A When inserting the lamp ensure that the cables are inside the two metal clamps This prevents the cables from getting caught during insertion Transmitted light Base TL BFDF Controls Adapter at the focusing drive 1 Adapter plate for easy assembly of focusing drives 2 Standard stage 10 447 269 3 Button to toggle between bright field and dark field Connector for cold light sources Button to toggle between bright field dark field light conductor active f 10mm end tube f 13mm Leica M series Manual 78 Transmitted light Base TL BFDF Operation Light intensity control Please observe the user manual in A particular all safety regulations from the manufacturers of the light guide and cold light source Switch on the cold light source according to the manufacturer s user manual and adjust the brightness Transmitted light control The TL BFDF transmitted light base has a poten tiometer that switches the light from bright field to dark field Leica M series Bright field Bright field is suitable for examining translu cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background Turn the control as far as it will go towards BF bright field Fingertip with bright field illumination Manual Dark field
17. 2 they will go and check to ensure that they Magnification range fit tightly and accurately You can extend the overall magnification range 2 Removethe plastic tube guard using available 10x 16x 25x and 40x wide field eyepieces for persons wearing glasses 4 Securely tighten the clamping screws Leica M series Manual 23 Objective Tools used No tools required Preparation 1 Remove the protective cap on the optics carrier by turning it Leica M series Attaching the objective Hold the objective firmly during assem bly and disassembly so that it does not fall onto the stage plate This applies particu larly to the 2x planapochromatic objective which is very heavy Remove all specimens from the stage plate first 2 Screw the objective clockwise into the optics carrier Manual Alternative fastening options If using the objective nosepiece read the instructions on page 25 If using the AX carrier read the instructions on page 27 24 Objective Nosepiece Assembly Preparations 2 Hold the objectives firmly during A assembly and disassembly so that they do not fall onto the stage plate Move the drive housing all the way upwards and remove the optics carrier if the carrier has already been installed Assembly 1 Remove the transport anchor from the objective nosepiece Leica M series Rotate the moving part by 90 and attach the objective nosepiece to the d
18. 3 Trinocular video phototube 50 66 TL RCI Methods in Transmitted Light 84 Trinocular video phototube 100 67 TL RCI Relief Images 85 Using Filters 87 soPro Non motorized Controls 88 The Microscope Carrier AX 69 IsoPro Motorized Controls 89 The Objective Nosepiece 71 Objectives andoptical accessories Leica LED5000 MCI 91 The Different Types of Objectives 73 Leica LED5000 RL 93 Leica M series ETTET 10 Accessories Leica PSC Controller Dimensional Drawings Leica M125 M165 C Leica M205 C a Leica LED5000 MCI 104 Leica LED5000 RL 105 Specifications for the Bases Transmitted light Base TL ST 107 Transmitted light Base TL BFDF 108 TL RC TL RCI 109 IsoPro Motorized XY Stage 110 Calculating the Total Magnification and Field of View Diameter 112 Care Maintenance Contact Persons 113 Leica M series Manual 11 LEICA 10450028 The Leica M Series Congratulations Congratulations on obtaining your new Leica M series stereomicroscope We are convinced it will exceed your expectations as never before have we applied our decades of experience in the areas of optics mechanical engineering and ergonomics in such an uncompromising manner The M series embodies all the qualities you associate with the name Leica Microsystems excellent objectives high quality engineering and reli ability Furthermore the modular design ensures that the M series adapts perfe
19. Leica partner and naturally you want to get right to work Therefore your next step should be to study the Quick Start Guide which outlines the most important steps at a glance This manual will then familiarize you with the finer details of your micro scope The following pages contain important practical information that makes using it every day easier Take time to read it it s worth it micabinatita The M series Quick Start Guide included with instrument Leica M series Manual Overview of an M series Microscope Click an entry or a position on the microscope to go directly to the corre sponding part of the manual Magnification changer zoom Switch click stops on off Coarse fine focusing Fastening screw for the binocular tube or accessories Interchangeable objective Adjustable eyepiece tubes 7 Eyepieces for spectacle wearers with dioptric correction and eyecups 8 Fastening screws for the eyepieces 9 Trinocular tube 10 Built in iris diaphragm a LUNS NN Leica M series Manual 48 The Correct Interpupillary Distance The interpupillary distance is correctly set if you Adjusting the Interpupillary Distance see a single circular image field when looking 1 Look into the eyepieces at a specimen 2 Hold the eyepieces with both hands Push If you are still a novice microscope user you the eyepieces together or separate them may need a short time to become accustomed until
20. age 1 Take the glass plate from the rectangular gap in the standard stage Position the stage on the transmitted light base in such way that the four holes align over those in the base Attach the stage to the base using the four Allen screws provided Insert the glass plate back into the standard stage Manual 32 IsoPro Manual Cross stage Assembly IsoPro Cross stage Before the IsoPro cross stage is mounted to the base the axis containing the control buttons is attached either on the left or the right side of the cross stage If the controls are to be mounted on the left hand side the gear rod on the bottom side of the cross stage must be unscrewed and reat tached in reverse 1 Take the glass plate from the cross stage 2 Turn the cross stage around and place it onto a non slip surface 3 Change the gear rod from the left to the right hand side 4 Skip the next two steps to mount the controls Leica M series Before the IsoPro cross stage is mounted to the base the axis containing the control buttons is attached either on the left or the right side of the cross stage Manual Left or right operation If the controls are to be mounted on the left hand side the gear rod on the bottom side of the cross stage must be unscrewed and reat tached in reverse 33 Take the glass plate from the cross stage and turn it around Change the gear rod from the left to the ri
21. ch between two objectives using just one hand movement for example between a 1x and a 2x planapochromat Leica M series Parfocal work With the new M series objectives the parfo cality is maintained even when objectives are changed meaning that the specimen remains in sharp focus during the change H Older Leica objectives can continue to be used but without parfocality during the objective change Manual Technical constraints The objective nosepiece cannot be A used together with the LED5000 MCI system illumination as the objective nosepiece can no longer be rotated 71 Objectives and optical accessories The Different Types of Objectives To meet the various requirements regarding imaging properties there is a choice of high quality interchangeable planachromatic and planapochromatic objectives and also lower priced interchangeable achromatic objectives Achromatic objectives are particularly suited for specimens with high contrast Structures Flat field planachromatic objectives are particularly well suited for studying flat objects such as wafers and thin sections X With planapochromatic objectives the finest structures are visible with high contrast The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity Leica M series Achromatic objectives The 0 32x 0 5x 0 63x 0 8x 1x 1 5x 2x achromatic objectives
22. ctly to your needs no matter which accessories you require for your tasks Leica M series Though the reliability and robustness of Leica stereomicroscopes is legendary like any high tech product the M series requires a certain degree of care and attention Therefore we recommend that you read this manual It contains all the information you need regarding operation safety and maintenance Simply observing a few guidelines will ensure that even after years of intensive use your stereomicroscope will continue to work as smoothly and reliably as on the very first day We wish you the best of success in your work after all you are now equipped with the best tool Manual 13 A Step Towards Infinity Ever since their introduction by Horatio S Greenough stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe s research For over a century ingenious optics designers and engi neers have worked to push magnification reso lution and image fidelity to the limit permitted by optics In doing so they have always been constrained by the interrelation between three factors the higher a microscope s resolution the lower the available working distance If one increases the distance of the optical axes the three dimen sional image seen by the observer becomes distorted a sphere becomes an ellipse a flat surface curves toward the observer Leica M205 C with 1 0x planapochromat and 10x
23. e cross stage on the base 2 Pull the upper part of the cross stage care fully toward the user fastening the lower part onto the transmitted light base 3 Screw the cross stage evenly onto the three threaded holes 4 Push the cross stage all the way back towards the column 5 Reinsert the glass plate back into the stan dard stage Manual 38 IsoPro Motorized Cross stage Assembly continued Cross stage for X Y Stage Control Module As soon as all instruments have been plugged 1 PlugtheCTL2 plug of the cross stage motor into the control box and it has been plugged into one of the three available CTL2 inter into the power supply the cross stage initializes faces and automatically drives to the mid position 2 Plug the CTL2 plug of the Leica PSC control 4 Connect the PC and X Y Stage DCI Module ler into another CTL2 interface with a suitable USB cable The third CTL2 interface is available for connecting other instruments such as the Leica foot switch 10 447 398 or TL RCI transmitted light base 10 446 352 The 15 pin Sub D interface is intended for use with the Leica SmartMove controller 11 501 197 3 Plug a power cable into the socket provided and into a grounded power socket Leica M series Manual 39 Cables Terminals The new Leica M series features extensive encoding with which various microscope data and settings can be read out transferred to the PC and reproduced late
24. e quality of your results Puta dust cover over the instrument when it will not be used for a long time wx Use dust caps to protect tube openings tubes without eyepieces and eyepieces Keep accessories in a dust free place when not in use Leica M series Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuitable cleaning agents and techniques can damage polymers Manual Permitted measures Clean the microscope or parts of it using warm soapy water then wipe using distilled water For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow the corresponding safety instructions Remove dust with a pneumatic rubber bulb or with a soft brush Clean objectives and eyepieces with special optic cleaning cloths and with pure alco hol 114
25. eased contrast without relief is attained if both diaphragms are set to 45 A gap like illuminated area is created By tilting the deflec tion mirror slightly you can move the gap over the entire field of view and quickly toggle between positive and negative relief images The dynamic effect makes it easy to distinguish phase structures from amplitude structures 83 TL RCI Methods in Transmitted Light Vertical bright field illumination Suitable for stained amplitude specimens with sufficient contrast The light beams are deflected vertically through the specimen This results in an accurate bright field with maximum brightness Leica M series Inclined transmitted light Suitable for semitransparent opaque speci mens such as foraminifera and fish eggs Move the deflection mirror until the desired data are visible Manual Single sided dark field Suitable for fixed specimens and fine struc tures The flatter the angle at which the light beams are deflected into the specimen plane the darker the substrate appears A dark field like transmitted light is created Outlines fine edges and structures are bright in contrast with the dark background through diffraction of the light beams on the dark background TL RCI Relief Images Starting position Positive relief contrast Negative relief contrast Suitable for semitransparent and transparent Suitable for semitransparent and transparent 1 Push the def
26. ect posi tion Manual 3 Push the Leica LED5000 MCI onto the retaining stirrup 44 Leica LED5000 RL The ring illuminator the Leica LED5000 RL Ring Light is installed on the objective using a single screw It has been optimized for a work ing distance between 60 and 70mm Constraints The Leica LED5000 RL can be used in conjunc tion with the planapochromat 1x and planapo chromat 0 63x objectives With all other objectives the working distance is too low for adequate illumination The ring illuminator cannot be used together with the objective nosepiece Leica M series Assembly 1 Connect the CAN bus cable to the A ring illuminator The flat part of the plug must be facing upwards A 2 Push the ring illuminator over the objective as far as it will go and screw it into place Manual A 3 Plug the other end of the cable into one of the two CTL2 sockets on the column For optimum accessibility of the specimen the ring illuminator should be installed with the cable facing backwards However it is also possible to turn the ring illuminator sideways for example if simultaneously using the Leica LED5000 MCI system illumination In this case the ring illuminator cannot be connected directly to the Leica LED5000 MCI 45 Quick Start Guide The Fastest Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your
27. ed by the LAS Leica Application Suite software Maximum brightness Maximum contrast Point illumination Point illumination Point illumination from the rear from the left from the right Leica M series Manual 92 Leica LED5000 RL Use The Leica LED5000 RL Ring Light generates a very bright and homogenous incident light It has a diameter of 80 mm and illuminates the specimen with 48 LEDs that can be switched on and off completely or in various combinations It is controlled using either the integrated or via the Leica Application Suite LAS LAS enables you to create fully reproducible illumination scenarios and automatically toggle between them For additional information refer to the LAS online help Leica M series Using the keyboard Use the key to switch the illumination on or off Use the and keys to adjust the brightness in 10 increments Touch either of the two buttons to adjust the intensity in small increments Hold a key to change the intensity more quickly Manual The illumination scenarios Use the and gt keys to toggle between the illumination scenarios shown below Constraint The Leica LED5000 RL has been designed for use with the 1 0x and 0 63x objectives For the 1 6x and 2 0x conventional ring illuminators from the Leica illuminator product range are available 93 Accessories Leica PSC Controller Use The Leica PSC controller gives you p
28. ents for parfocal work If you are using an eyepiece with dioptric correction the procedure differs from this description For more information refer to page 61 For the procedure for adjusting the parfo cality for the objective revolver refer to page 26 Leica M series Parfocality 1 Enlarge the view to the maximum level 2 Focus on the specimen You are done Even if you select a smaller work ing distance the specimen remains pin sharp The parfocality is maintained until you focus on another level of the specimen Manual 55 Iris Diaphragm The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camera shutter it regulates the avail able light which changes the depth of field The depth of field is the area of a specimen that is brought into sharp focus Leica M series Closing the iris diaphragm Close the iris diaphragm by turning the knob to the left The subject appears darker and the depth of field increases Opening the Iris Diaphragm X Open the iris diaphragm by turning the knob to the right The subject now appears brighter but the depth of field decreases Manual Eyepieces Magnification Factors of the Eyepieces An eyepiece not only makes it possible to look passively into the micro scope but also has a critical effect on the maximum magnification The magnification factor is between 10x and 40x The foll
29. epieces covered 2 Focus onthe specimen X LeicaM125 16 5 1 Leica M165 C 16 5 1 3 Rotate the magnification changer until the Leica M205 C 20 5 1 desired magnification is configured The rotary knob for the zoom can be used either left or right handed Leica M series Manual 53 Ratchet Steps and Magnification Levels The zoom button can optionally be operated either with or without ratchet steps Continu ous zoom is possible when the ratchet steps are disabled which many users find convenient On the other hand when the ratchet steps are enabled photographs measurement results etc can be reproduced more accurately Leica M series Enabling and disabling ratchet steps 1 Push the top button downwards to disable the ratchet steps 2 Push the bottom button upwards to enable the ratchet steps Manual Magnifications and fields of view The formula on page 112 provide additional information about the magnifications and field of view diameters with consideration given to the position of the magnification changer and the eyepiece and objective combination used Parfocality More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched meaning that you can view a focused specimen from the lowest to the highest magni fication without having to refocus There is no need to readjust the focus to view of specimen location that is located higher or lower Requirem
30. ght hand side Leica M series IsoPro Manual Cross stage Assembly continued Control assembly 1 Take the glass plate from the cross stage and turn it around Attach the axis with the control buttons to the desired side The fastener snaps into the cross stage magnetically Attach the axis with the two Allen screws provided Attach the cover rail to the cross stage Manual Cross stage assembly 1 Place the cross stage on the base Pull the upper part of the cross stage care fully toward the user fastening the lower part onto the transmitted light base IsoPro Manual Cross stage Assembly continued 3 Screw the cross stage evenly onto the three Focusing drive and column threaded holes 1 Unscrew the extension plate from the base using the Allen key provided 4 Push the cross stage all the way back towards the column 2 Attach your focusing drive column to the bottom using the three Allen screws 5 Reinsert the glass plate back into the stan dard stage 3 Reattach the adapter plate to its original position using the three Allen screws Leica M series Manual 35 lsoPro Motorized Cross stage Assembly Basics 1 Unpack the cross stage from the transport 2 Secure the motorized cross stage to the The transmitted light bases of the TL series packaging and position it on the transmit base using 3 M4 screws TL BFDF TL RC TL RCI are supplied with ted light base an installed ex
31. ibilities of person in charge of instrument Xx Ensure that the Leica stereomicroscope is operated maintained and repaired by authorized and trained personnel only Safety concept The individual modules of the Leica M stereo microscopy series include an interactive CD ROM with all relevant user manuals in 20 other languages Keep it in a safe place and readily accessible to the user User manuals and updates are also available for you to download and print from our web site at www stereomi croscopy com This operating manual describes the special functions of the individual modules of the Leica M stereomicroscopy series and contains impor tant instructions for their operational safety maintenance and accessories The Safety concept booklet contains addi tional safety information regarding the service work requirements and the handling of stereo microscope accessories and electrical accesso ries as well as general safety instructions Leica M series You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the user manual and the safety requirements of the supplier Before installing operating or using the instru ments read the user manuals listed above In particular please observe all safety instruc tions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings con
32. ica M125 M165 C with transmitted light stand TL RC manual cross stage Leica IsoPro and manual focus dimensions in mm 292 max 370 sail e I te 4__ 4 G max 370 PA X 75 mm Leica M series Manual 100 Leica M205 C Leica M205 C with transmitted light stand TL ST and manual focusing drive dimensions in mm ag I qui l ST oS ZZZ DO se SNZ OO i 370 al Leica M series Manual 101 Leica M205 C continued Leica M205 C with transmitted light stand TL RCI and manual focusing drive dimensions in mm Leica M series Manual 102 Leica M205 C continued Dimensions of Leica M205 C with transmitted light stand TL RCI manual cross stage Leica lsoPro and manual focus dimensions in mm Pas E E 8 o S N lt N max 410 X 75 mm 103 Manual Leica M series Leica LED5000 MCI Leica LED5000 MCI dimensions in mm Leica M series Manual 104
33. ica incident light base and TL bases TL BFDF TL RC TL RCI Stage size LxWxH 335 5 mm x 370mm x 41 5 mm Travel 152 mm x 102 mm 6 x 4 Speed X Y 30 mm s Motor resolution X Y 0 25 um Repeatability X Y 2 um Positioning accuracy X Y 20 um over the entire travel range Hysteresis X Y 30 um Motor Direct current DC motor with encoder Backlash Slack free Adapter All adapters for TL bases Acceleration S shaped acceleration Computer connection Control options USB Leica PSC Leica Smart Move Leica LAS via PC Power supply Input 100 240 VAC 50 60 Hz 1 0 A Output 15 VDC 2 66 A 40W MAX Leica M series Manual 110 Appendix Leica M series Manual 111 Calculating the Total Magnification and Field of View Diameter Parameter Example MO Magnification of objective MO 1x objective ME Magnification of eyepiece ME 25x 9 5 eyepiece Z Magnification changer position Z Zoom position 4 q Tube factor e g 1 5x for coaxial incident light 1 6x for 45 q Coaxial reflected light 1 5x tube factor ErgoTube r Factor 1 25x r Factor 1 25x if the planachromatic and planapochromatic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ75 or MZ95 NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Calculation example magnification in binocular tube Calculation exam
34. icroscope carrier AX for stereoscopic axial observation The parallax free imaging by means of a perpendicular beam path leads to results without parallax error Compatibility The planachromatic and planapochromatic objectives fit into the thread in the microscope carrier AX for the Leica M125 Leica 165 Cand the Leica M205 C Leica M series Stereoscopic viewing A stereoscopic view is required for examining Spatial objects For this purpose the micro scope carrier AX must be brought to its home position 1 Unscrew the clamping screw 2 Push the microscope into the home posi tion mid position to obtain a spatial view and tighten the clamping screw Manual 69 The Microscope Carrier AX continued Parallax free images The parallax free imaging produces more precise results in photography measuring and polarization 1 Unscrew the clamping screw 2 Push the microscope towards the left as far as it will go and tighten the clamping Screw Leica M series The objective is now located directly beneath either the left or the right beam path Coaxial light and the Leica M205 C When using coaxial incident light together with the Leica M205 C unscrew the clamping screw and press the optics carrier towards the right caution the movement is only 2mm This provides uniform light intensity in both beam paths Manual The Objective Nosepiece Use The objective nosepiece enables you to swit
35. ing drive of the M series to the corresponding base 1 Securely install the column adapter on the 2 Securely screw the focusing column to the column using the four included screws base using the six included screws Tools used Hex socket screwdriver 3mm Leica M series Manual 20 Optics carrier Tools used Assembling the optics carrier Hex socket screwdriver 4mm 1 Place the optics carrier on the focusing 2 Press the optics carrier backwards to the column so that the screw fit into the thread focusing column and screw it in place using provided and the lug fits into the groove your other hand Leica M series Manual 21 Tube All intermediate tubes that fit between the Assembling the tube optics carrier and the binocular tube are fitted 2 Push the tube for example the inclined in the same manner binocular tube into the dovetail ring and rotate it slightly in both directions until the Tools used positioning screw meshes with the guide No tools required groove Preparations 1 Unscrew the positioning screw and remove the protective cover Leica M series Manual While holding the tube only slightly care fully tighten the positioning screw It is automatically brought to the correct position 22 Eyepieces Tools used Preparation Inserting the eyepieces No tools required 1 If you want to use an optional graticule 3 Push the eyepieces into the tubes as far as insert it now page 6
36. ing the color No temperature Matching of high num aperture Yes Remote control options Yes AntiShock Pads Yes Dimensions WxHxD 340x390x90mm With cold light source Leica CLS150 LS Leica M series Concave mirror With external light source Manual 108 TL RC TL RCI Leica TL RC Leica TL RCI Light source External via cold light source Halogen lamp 12V 20W Quick illuminant change Yes Illuminated area 35mm 35mm Power supply unit 100 240 V frequency 50 60 Hz energy consumption 30 W max ambient temperature 10 40 C Connections Connection for cold light guide active f 10 mm end tube f 13 mm 1XUSB type A 1xUSB type B 2xCAN BUS Weight 6 0 kg 7 2 kg Illumination types Bright field dark field Yes yes Yes yes Oblique light Relief Contrast System RC Yes yes Yes yes CCIC Constant Color Intensity Control No Yes Internal shutter lamp control Yes Yes Integrated filter holder Yes Yes Coated optics for increasing the color temperature Yes Yes Matching of high num aperture Yes Yes Remote control options Yes Yes AntiShock Pads Yes Yes Dimensions of base WxHxD 340x390x95 mm 340x440x95 mm Single sided With cold light source Leica CLS150LS Concave mirror With external light source Leica M series Manual 109 IsoPro Motorized XY Stage Compatibility Le
37. lection mirror all the way back specimens The phase structures look like hills specimens These settings result in a nega towards the column tive relief contrast Phase structures look like The effect can be strengthened or weakened by valleys 2 Turn the deflection mirror into the notch gently tilting the deflection mirror position at an angle of 45 The effect can be strengthened or weakened by gently tilting the deflection mirror Leica M series Manual 85 TL RCI Relief Images continued Dynamic relief contrast Suitable for semitransparent and transparent specimens By tilting the deflection mirror slightly you can move the gaps over the entire field of view and quickly toggle between positive and negative relief images The dynamic effect makes it easy to distinguish phase structures from amplitude structures Leica M series Constraints The relief methods provide good results from mid zoom to high magnifications and with 1x 1 6x and 2x magnifications In the lower zoom half and with weaker specimens the object field may not be uniformly illuminated We recommend using the transmitted light base with 1x or higher objectives and not objectives with a long focal length Manual 86 Using Filters Filters for TL RC and TL RCI The transmitted light bases TL RC and TL RCI can be equipped with up to three filters available as accessories simultane ously By customer request the fi
38. lters are also available as one off items 1 Switch off the light source or click TL RCI the button for the shutter Te sie n Leica M series 2 Take the empty filter from an available filter slot in the filter holder 3 Insert the desired filter 4 Switch the light source back on Manual Daylight filter for TL ST A daylight filter is also available for the transmitted light Base TL ST 87 IsoPro Non motorized Controls Operating the cross stage IsoPro 1 To move the stage in X direction rotate the outer knob 2 To move the stage in Y direction rotate the inner control ring Leica M series Manual 88 IsoPro Motorized Controls IsoPro 1 Motorized cross stage Leica IsoPro 2 Housing with motorization Leica M series X Y Stage DCI module Leica PSC Controller N UNS Sub D interface for Leica SmartMove 1 Quickcontrol memory function 3 CTL2 interfaces 2 Fine control in X direction USB interface type B 3 Fine control in Y direction Socket for grounded power cable Manual 89 System Illumination Leica LED5000 MCI Use The Leica LED5000 MCI for Multi Contrast Illumination is a universal high output illu minator Three groups of 3 LEDs each can be arranged as desired around the specimen The targeted control of the LEDs enables various illumination scenarios to be implemented Preparation Hold the MCI with both hands and
39. moved The mirror is kept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light Manual Bright field Bright field is suitable for examining translu cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background Move the slider backwards until the desired effect is achieved Inclined transmitted light Transmitted light that traverses the object obliquely will produce effects advantageous for observing semitransparent opaque objects Slowly pull the slider towards yourself until the desired effect is achieved 76 Transmitted light Base TL ST Lamp Replacement Changing the Halogen Lamp Before you change the lamp it is abso lutely necessary to unplug the power plug from the base to prevent the risk of elec tric shock The halogen lamp becomes very hot during operation Therefore to avoid being burned let the base cool off for approx 10 minutes after switching it off A Do not touch new halogen lamps with your bare fingers this drastically reduces the service life of the lamp Leica M series Changing bulbs 1 Unscrew the two screws on the cooling unit and pull the cooling unit out along with the lamp 2 Carefully pull out the lamp and mount by pulling them upwards 3 Disconnect the lamp from the mount 4 Insert the new lamp into
40. offer countless variants for selecting the object field diameter magni fication ranges and working distances see page 112 Planachromatic objective 1x For the highest requirements for overall image quality we recommend equipping the micro scope with the 1x plan flat field objective which returns sharp contrast rich object fields Manual Achromatic objectives with a long focal length For special applications achromatic objectives with long working distances and focal lengths of f 100 mm to 400 mm are available 73 Leica M series Manual 74 Transmitted light Base TL ST Controls Extension plate ofthe Transmitted light Base TL ST Rear side of the transmitted light base TL ST 1 Screws for changing the halogen lamp 2 Power connection socket 1 Adapter plate for easy assembly of focusing 3 Power switch drives 2 Removable glass plate Controller for light intensity 4 Adjustment for deflection mirror Uu Leica M series Manual 75 Transmitted light Base TL ST Operation Light intensity control The left control adjusts the intensity of the 12V 20W halogen illumination 1 Switch on the illumination of the base at the power switch 3 Focuson the specimen 3 Set the illumination to the desired intensity using the left control Leica M series Transmitted light control The transmitted light base TL BFDF has a slider that automatically moves the deflection mirror in the base when
41. or the digital camera to capture as much of the field of vision as possi ble while excluding as much of the black edge of the field of vision as possible To do so the magnification factor of the C mount adapter must match the image format of the sensor as closely as possible see table Leica M series If there is unwanted shading at the corners even with a compatible C mount adapter installed it can be corrected using the Shading function of the camera software Alternatively you can also use a C mount adapter with higher magnification This primar ily avoids the critical border area of the field of vision and concentrates on the center of the field of vision Camera optimal suitable large smaller image field image field DFC290 0 5x 0 63 DFC420 0 5x 0 63x DFC490 0 63Xx 0 8x DFC500 0 63X 0 8x Manual Cameras from third party suppliers In addition to Leica DFC cameras with the stan dardized C mount interfaces you can connect third party cameras to the microscope using a T2 bayonet adapter To do so instead of the C mount adapter simply use the correspond ing SLR adapter with T2 connection However these third party cameras are not integrated into the Leica Application Suite and have to be operated using the corresponding software from the camera manufacturer The Leica digital cameras are detailed in a sepa rate user manual along with instructions for their assembly and use 65
42. osion UV exposure blinding Before opening the lamp housing allow it to cool off for at least 15 minutes Danger of explosion Never cover the air duct on the lamp hous ing Danger of fire Manual Mercury lamp Follow the user manual and safety instruc tions provided by the lamp manufacturer and in particular the section on how to proceed if a lamp breaks and releases mercury For transport remove the mercury lamp transport it in its original packaging and protect moving parts in the lamp housing using the transport anchors To minimize the risk of explosion replace a discoloured mercury lamp promptly when it has reached the end of its rated life follow the manufacturer s specifications and the minute meter on the supply unit Leica Microsystems assumes no liability for damage caused by exploding incor rectly installed or improperly used mercury lamps Table of Contents General Instructions 2 Transmitted light Base TL BFDF Before First Use 30 Safety concept 3 Transmitted light Base TL BFDF 31 Symbols used 4 TL RC TL RCI 32 Safety regulations 5 IsoPro Manual Cross stage Assembly 33 IsoPro Motorized Cross stage Assembly 36 Cables Terminals 40 Congratulations 13 Cables Cable Duct 41 A Step Towards Infinity 14 Cables Diagram 42 The Electronics Comfort Convenience and
43. oss stage Leica IsoPro automatic cross stage Standard stage 10 447 269 Leica M series The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Manual Standard stage 1 Take the glass plate from the rectangular gap in the standard stage Position the stage on the transmitted light base in such way that the four holes align over those in the base Attach the stage to the base using the four Allen screws provided Insert the glass plate back into the standard stage 31 TL RC TL RCI The base is delivered with the adapter plate installed The selected stage IsoPro cross stage or standard stage 10 447 269 and the focusing drive will have to be mounted later Ensure that the instruments are unpacked on a level adequately sized and nonskid underlay Stage assembly The transmitted light base TL RC RCI can be equipped with three different stages The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand move ments The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Leica M series Standard st
44. owing eyepieces are available for the M series Magnification Dioptric correction Order number 10x 5 diopter settings 10 450 023 16x 5 diopter settings 10 450 024 25x 5 diopter settings 10 450 025 40x 5 diopter settings 10 450 026 Leica M series Manual 58 Health Notes Potential sources of infection Direct contact with eyepieces is a A potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepieces or detachable eyecups Eyecups can be ordered separately Please contact your Leica partner fama s TO sg H gI Ye 29 fel Pi le inf es g t In 73 73 TICA 7048 ae CCA 1045007 Separate eyecups are an effective way of preventing infections Leica M series Manual 59 Dioptric correction All Leica eyepieces are also available with built in dioptric correction allowing the micro scope to be used without glasses even by those with vision problems The correction comprises 5 diopter settings Leica M series Using the Dioptric Correction 1 Set the dioptric correction of both eyepieces to the mid position 0 diopter settings While wearing your glasses look through the eyepieces and focus on the specimen Rotate both eyepieces to the maximum value of 5 Hold one eye closed and rotate the other eyepiece in direction until the specimen appears sharp Then open the other eye and correct the
45. ple field of view diameter in the specimen MTOTVIS MO x MExZXxQqxr or 1x25x4x 1 5 x 1 25 187 5x Leica M series Manual 112 Care Maintenance Contact Persons We hope you enjoy using your high perfor mance microscope Leica microscopes are renowned for their robustness and long service life Observing the following care and clean ing tips will ensure that even after years and decades your Leica microscope will continue to work as well as on the very first day Warranty benefits The guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Leica M series Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Switzerland AG CH 9435 Heerbrugg E mail contact stereo service leica microsystems com Manual Care Protect your microscope from moisture fumes and acids and from alkaline caustic and corrosive materials and keep chemicals away from the instruments Plugs optical systems and mechanical parts must not be disassembled or replaced unless doing so is specifically permitted and described in this manual Protect your microscope from oil and grease Do not grease guide surfaces or mechani cal parts 113 Care Maintenance Contact Persons continued Protection from dirt Dust and dirt will affect th
46. pull it down wards until the illuminator clicks into place on the bottom end of the guide rods In this position you always have the same contrast with identical illumination This guar antees the reproducibility of an experiment Leica M series Installed too high Optimum height Manual Contact with the base If the optics carriers are accidentally lowered too far contact between the base and MCI may result A safety mechanism in the linkage ensures that in this case the MCI is automatically pushed upwards to prevent the possibility of damage Be sure not to place any specimens directly beneath the MCI After the optics carrier is lifted put the MCI back into the original position 91 Leica LED5000 MCI continued Using the keyboard The illumination scenarios Use the key to switch the illumination on Use the and gt keys to toggle between the LEDSO00 MCI Scene 1 50 or off illumination scenarios shown below X Use the and keys to adjust the Maximum brightness brightness in 10 increments Touch either For temperature reasons its is not possible to of the two buttons to adjust the intensity in switch on all nine LEDs simultaneously There small increments Hold a key to change the fore to provide bright overall illumination the intensity more quickly top two rows are activated The bottom row primarily provides contrast The Leica LED5000 MCI can also be controll
47. r The interface to the optics carrier is on the column Leica M series The Terminals Manual The connection to the PC and to other instru ments is made using the terminals on the rear side of the column 1 2xCTL2 terminals for auxiliary equipment such as the ring illuminator the base and other accessories from the Leica product range 2 Terminal for the 50 watt power supply provided 3 USB terminal for the connection to the PC 40 Cables Cable Duct The integrated cable duct in the column enables a neat cable layout around the microscope For example the USB or FireWire cables of the camera can be stowed in the cable duct Leica M series Feeding the cables 3 Place the cables in the cable duct and screw 1 Unscrew the three screws on the cable the cover on tightly duct Tip Estimate the length of the cable ends you 2 Remove the cover of the cable duct will need before screwing on the cover For thick cables it is difficult to change the length retroactively Manual 41 Cables Diagram ari Alternative installation Please use the Leica Application Suite to configure the functions of the system DCI Controlbox Power USB 115 230V 0000000000000 0000000020 0K09LKKKOLKYLLKKEKKYKKKKIYKKKLYIKKKKCKYKIYKKKLIKYIKKKKKYYYKYKKLKLLKKYKCKCKCKE CCC00000000000c00c00sc0cc000s000lfocccccccoscscccccscccoccsce 0 0e 0000000000000 000000000000000
48. recision control of the Leica IsoPro motorized cross stage Connection Connect the Leica PSC controller and the Leica IsoPro motorized cross stage Leica M series Passing over the specimen quickly Move the joystick in any desired direction including diagonally to quickly control the cross stage Fine control of the cross stage The motorized Leica IsoPro cross stage offers an accuracy of up to 0 25um To move to a posi tion with maximum accuracy use the left and right knobs on the joystick Manual Control by LAS The instructions for controlling the Leica IsoPro using the Leica Application Suite are provided in the software s help file 95 Dimensional Drawings Leica M125 M165 C Leica M125 M165 C with incident light base and new focusing column dimensions in mm Leica M series Manual 97 Leica M125 M165 C continued Leica M125 M165 C with transmitted light base TL ST and manual focus dimensions in mm Leica M series Manual 98 Leica M125 M165 C continued Leica M125 M165 C with transmitted light stand TL RC and manual focus dimensions in mm Leica M series Manual 99 Leica M125 M165 C continued Le
49. rive hous ing from the front Screw the objective nosepiece firmly into place Unscrew the three Phillips screws on the objective mount of the optics carrier and remove the intermediate ring Manual Screw the optics carrier onto the objective nosepiece Screw both objectives onto the objective nosepiece It makes no difference which position an objective occupies Unscrew the locking screws on both sides of the objective nosepiece You can now adjust the parfocality see instruc tions on the next page 25 Objective Nosepiece Adjusting Parfocality The following procedure only has to be carried out once Afterwards both objectives are parfo cal meaning that the specimen remains in focus when the objective nosepiece is rotated This procedure must be repeated if you replace either of the two objectives with another The following example assumes the combi nation of the 1x and 2x planapochromats If you are using another objective combination replace the 2x objective in the description with the objective with the stronger magnification Preparation Open the iris diaphragm X Set the dioptric correction of the eyepieces to o Leica M series Adjustment 1 Rotate the 2x objective into the beam path and set it to the lowest magnification Focus on the specimen Rotate the 1x objective into the beam path Turn the objective on the thread in both directions until the specimen
50. side and can be rotated and moved The concave side has been specially designed for the optical requirements of objectives with a high numerical aperture The black rotary knob on the left side of the transmitted light base can be used to rotate the built in path folding mirror and move it forwards back The concave cutout on the handle indicates the concave side of the mirror allowing intuitive operation at any time without eye contact The angle of light incidence in the specimen plane changes depending on the tilt and posi tion of the mirror As a result switching between transmitted light bright field oblique illumina tion and dark field like illumination is possible Manual Functions of the rotary knob The rotary knob 1 fulfills the following tasks Turning the deflection mirror from the flat to the concave side Slight tilting to guide the light beam through the specimen plane at a steeper or flatter angle Moving the deflection mirror forwards back 81 TL RCI Color Intensity and Temperature The transmitted light base TL RCI has two electronic potentiometers that control the color intensity 1 and color temperature 2 The controller for the color temperature simul taneously serves as an electronic shutter To interrupt your work click the potentio meter 2 Click again to switch on the illumination The electronics return to the previously 1 Potentiometer for controlling the
51. t Leica M series 2 Install the AX carrier on the focusing column 3 Screwthe objective to the AX carrier Manual 4 Unscrew the three holding screws and amp remove the adapter ring on the optics carrier Install the optics carrier on the AX carrier 28 Transmitted light Base TL ST Unpacking the base The base is delivered with the adapter plate installed Make sure the devices are unpacked on a flat sufficiently dimensioned and non slip surface Leica M series Focusing drive and column Unscrew the extension plate from the base using the Allen key provided 1 Manual Attach your focusing drive column to the bottom using the 4 Allen screws Reattach the adapter plate to its original position using the 6 Allen screws Transmitted light Base TL BFDF Before First Use Removing the transport anchors Before you can use the transmitted light base for the first time it is abso lutely necessary to remove the two transport anchors as follows Anchor of mirror Anchor of switching slide Leica M series Manual 30 Transmitted light Base TL BFDF Standard delivery The base is delivered with the adapter plate installed The selected stage IsoPro cross stage or standard stage 10 447 269 and the focusing drive will have to be mounted later Stage assembly The TL BFDF transmitted light base can be equipped with three different stages Leica IsoPro manual cr
52. t level the specimen is always brought into sharp focus If not repeat the process 61 Graticules Use Graticules make length measurements and counting easier particularly for workstations that are not equipped with a digital camera and LAS software The graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces 1 Screw the insert off of the eyepiece Leica M series 2 Clamp the graticule on the insert applying moderate pressure Ensure that the grati cule fits tightly 3 Screw the insert and graticule firmly into place and replace the eyepiece in the tube Manual 4 You can now align the graticule by rotating the eyepiece in the tube and then tighten ing it using the clamping screw Use with the AX carrier If possible measure with the micro H scope carrier AX in vertical position The measurements are more accurate without the convergence angle in the stereoscopic image 62 Photography amp Video Photography amp Video For most microscope users digital documen tation has become an invaluable part of their work Research results can be presented in an attractive manner measurements on the digi tal image provide clarity and in conjunction with the motorized IsoPro cross stage even images of large specimens can be captured step by step and automatically joined to create a new complete image Adapter If camera con
53. tained in these user manuals Manual Symbols used Warning of a danger This symbol indicates especially impor A tant information that must be read and complied with Failure to comply can cause the following Hazards to personnel X Functional disturbances or damaged instru ments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that if not observed can cause the following Hazards to personnel Functional disturbances or damaged instru ments Leica M series Danger due to hot surface A This symbol warns against touching hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Action This symbol refers to actions described in the text that are to be carried out Figures 1 Numbers in brackets within the descrip tions relate to the figures and the items within those figures Manual Safety regulations Description The individual modules fulfill the highest requirements for observation and documen tation of Leica stereo microscopes of the M series Designated use See Safety concept booklet Non intended use See Safety concept booklet Never use M series microscopes or their compo nents for surgical procedures e g on the eye unless they are specifically intended for that purpose The devices and accessories
54. tension plate The selected stage IsoPro cross stage or standard stage 10 447 269 and the focusing drive will have to be mounted later The motorized cross stage is a sensitive preci sion instrument During installation avoid subjecting the stage to impact or severe vibra tions 1 Microscope base 3x M4 screws 2 Motorized cross stage Leica M series Manual 36 lsoPro Motorized Cross stage Assembly continued 3 Remove the two M3 screws and the holder 4 Remove the two M4 screws and the sleeve 5 Remove the four shock absorbing cartons from the cross stage from the cross stage from the cross stage H After removal keep all the trans port anchors in the plastic bag for future transport Leica M series Manual 37 lsoPro Motorized Cross stage Assembly continued The bases The TL BFDF TL RC and RCI transmitted light bases can be equipped with three different stages standard stage manual and IsoPro automated cross stage The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Leica M series Cross stage and base Never move the sledge of the motorized A cross stage manually in the X direction as otherwise the mechanical system will be damaged 1 Place th
55. trol using the Leica Application Suite is not required conventional mirror reflex and rangefinder cameras from third party manufacturers can be used For this purpose Leica Microsystems offers a variety of adapters that can be used together with the 50 and 100 trinocular tubes Leica M series Leica DFC cameras However if you require absolute control over the camera and need the capability for measurement evaluation and more in addition to photography the digital Leica DFC cameras are exactly right for you Together with the Leica Application Suite they provide virtually limitless freedom of use For additional informa tion about Leica cameras refer to the camera s documentation Manual Leica Application Suite The Leica Application Suite or LAS for short is as it were the digital extension of the Leica M series microscopes In addition to capturing images it lets you control the micro scope illuminator stages cameras and more For additional information refer to the LAS online help a 64 Photo tubes and C mounts Application All Leica DFC cameras are equipped with a standardized C mount interface In turn the C mount adapter for the respective trinocular tube is connected to this interface This adapter creates a solid mechanical connection between the microscope and camera and ensures opti mum rendering of the microscopic image on the image sensor of the camera Usually the ideal is f
56. truments switch off the power and unplug the power cable Legal regulations Touching the live circuit can cause injury See Safety concept booklet Transport EC Declaration of Conformity Use the original packaging for shipping or See Safety concept booklet transporting the individual modules of the Leica M stereomicroscopy series and the accessory components x In order to prevent damage from vibrations disassemble all moving parts that accord ing to the user manual can be assembled and disassembled by the customer and pack them separately Leica M series Manual Health risks Workplaces equipped with stereomicroscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken Optimal arrangement of workplace work assignments and work flow changing tasks frequently Thorough training of the personnel giving consideration to ergonomic and organiza tional aspects The ergonomic design and construction of the Leica M stereomicroscopy series are intended to reduce the exertion of the user to a minimum Safety regulations continued Direct contact with eyepieces can be a potential transmission path for bacterial and viral infec tions of the eye The
57. you see a circular image to this Not to worry after a little while it will become automatic Reference value The distance between eye and eyepiece measures approx 22mm for 10 23B wide field eyepieces for persons wearing glasses Leica M series Manual 3 Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting 49 Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer Simply push them into the tube and they are ready to use Each eyepiece offers a certain magnification factor that has a determinative effect on the total magnification Furthermore all Leica eyepieces can be equipped with practical grati cules that enable measuring and quantifying of specimens Dioptric correction A built in dioptric correction is available for eyeglass wearers For more information refer to page 60 Leica M series If you do not wear glasses 1 74 Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise If an eyepiece is equipped with the inte If you wear glasses 1 Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear as otherwise the viewing distance is too great grated dioptric correction turn the value to the 0 mark Manual 2 If an eyepiece is equipped with the inte grated dioptric correction turn the value to the 0 mark By the way
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