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1. of your Hamilton pipette Testing is performed at a Hamilton facility under stringent conditions on state of the art equipment The calibration process is performed with an unbroken chain of calibrations traceable to N I S T Your pipette will be returned to you with a new Certificate of Performance If a pipette must be repaired to bring it into conformance you will be contacted with a cost estimate Repairs Hamilton Company offers a pipette repair program in addition to its general 12 month warranty You may return your Hamilton pipette for evaluation A series of basic tests will be performed to evaluate the pipette s performance If repairs are required to assure and confirm that your pipette is functioning to original Hamilton specifications you will be contacted with a cost estimate and the additional repairs and recalibration will be performed upon your approval Your pipette will be returned to you with a new Certificate of Performance For pipette recertification and repairs in the U S A call 1 888 525 2123 For authorized service outside of the U S A contact your local Hamilton Company office or representative using the contact information on the back cover 10 of 12 888 525 2123 1773 01 Rev E Return of Goods Hamilton return and repair policy is written to protect our employees from potentially hazardous materials e g infectious agents radioactive materials and carcinogenic chemicals or any substance that may ca2. the best accuracy and precision use your pipette only within the recommended volume range of 10 100 of the specific pipette s total volume range Recommended Volume Ranges Pipette Volume 2ygL 10pL 25 100pgL 1mL Pipette Color Aqua Purple Forest Violet Brick Red Sky Blue Green asi haad 0 2 2 uL 1 10 pL 2 5 25 pL 10 100 pL 30 300 pL 0 1 1 mL ic n 2 000 10 00 uL 25 00 pL 100 0 pL 300 0 uL 1 000 mL Reading at 10096 Volume 1096 Volume Setting 0 200 uL 0 100 mL Reading at 10 Setting Smallest Increment 0 002 uL 0 02 uL 0 02uL 2 0 2 10 002 mL the Scale 4 of 12 888 525 2123 1773 01 Rev E Pipette Operation 1 Press a disposable tip firmly onto the 5 lower body assembly 2 Depress the plunger button to the first stop Hold the pipette vertically and immerse the tip 2 to 4 mm into the sample below the meniscus 3 Slowly release the plunger button to aspirate sample 4 Withdraw the tip from the sample touching off on the side of the container 5 To dispense place the tip against the inside wall of the receiving vessel and depress the plunger button to the first stop Pause and then depress the plunger button to the second or blowout stop Hold the plunger button down sliding the tip out along the inside of the receiving vessel 6 Release the plunger button With the disposable t
3. the pipette on its side with fluid in the tip Fluid reaching the piston causes contamination and possible corrosion Cleaning Piston Wipe piston with alcohol and a soft lint free cloth Dry and lightly lubricate the piston Lubricant Part No 5763 01 Seal Replacement Replace the PTFE seals every 3 6 months depending on use and the samples aspirated Performance Checks Check the accuracy and precision of your pipette every 3 6 months depending on use and the samples aspirated Cleaning Wipe the outer surface of the pipette daily with a mild soap and water In case of accidental contamination of the pipette shaft immediately remove the ejector sleeve and lower body assembly refer to Pipette Parts Diagrams Rinse the ejector sleeve and lower body assembly with a mild soap and water followed by distilled water Dry all components completely Lightly lubricate the piston Lubricant Part No 5763 01 Hold the plunger button down to engage the piston into the seal After reattaching the lower body assembly attach the injector sleeve over the lower body C ring 1773 01 Rev E www hamiltonpipettes com 7 of 12 Troubleshooting Problem Possible Cause Recommended Remedy Inaccurate Results Pipette tip not attached Check for tip fit and check for correctly proper tip size replacing tip as needed Aspiration stoke too rapid Aspirate slowly by controlling viscous fluids rate of
4. 2 Retainer Assembly contains 1 retainer and 1 C ring 9 Lower Body Assembly Includes 5 PTFE seals with O rings and 1 tube of lubricant Lower body assemblies are shipped with a seal kit 1773 01 Rev E www hamiltonpipettes com 9 of 12 Replacement Parts Table 20 5 pl 10 pL 25 pL 50 pL 100 pL 200 pL 250 pL 300pL 500 pL 1mL 54190 02 54190 13 54190 11 54190 03 54190 06 54190 17 54190 07 54190 08 54190 10 54190 04 54190 01 2 n a n a n a n a n a n a n a n a 34196 34196 3 n a n a n a n a n a n a n a n a n a 1288 06 1288 06 4 n a n a n a n a n a n a n a n a n a 34154 34154 ui 1288 12 1390 01A 1390 01 1390 01A 1390 01A 1390 01 1390 01 1390 01A 1390 01 1390 014 1390 01A 6 4029 001 34106 34106 34136 34062 34107 34012 34012 34012 34132 34133 7 nla 34064 34064 n a n a n a n a n a n a n a n a 8 1708 79 1708 70 1708 70 1708 71 1708 72 1708 73 1708 74 1708 75 1708 76 1708 77 1708 78 9 4118 07 4118 08 4118 09 4118 10 4118 11 4118 12 4118 13 4118 14 4118 15 4118 16 4118 17 10 54758 01 54758 01 54758 01 54757 01 54757 01 54757 01 54757 01 54757 01 54757 01 54756 01 54756 01 11 34189 34189 34189 34189 34189 34189 34189 34189 12 4028 01 Pipette Recertification and Repairs Recertification Hamilton Company offers a program to recertify the accuracy and precision
5. 23 1773 01 Rev E Optimizing Performance Topic Action Consistent Technique Pipette with a consistent rhythm pressure and speed Tip Selection Use Hamilton pipette tips for guaranteed accuracy For information visit www hamiltonpipettes com Tip Size and Fit Use proper size tip firmly placed on pipette shaft Pre Rinse Tips Pre rinse pipette tips for improved precision Sample Aspiration Keep the disposable tip immersed in fluid during aspira tion Do not let the plunger snap back to starting position Immersion Depth Maintain an immersion depth of 2 to 4 mm Immersion Angle Maintain an angle of lt 20 from vertical Viscous Samples Aspirate slowly If bubbles are observed resample Volume errors may still occur Refer to the Calibration section Acid Samples Pipetting strong acids and corrosive solutions is not recommended These liquids may damage the piston and seal To protect the pipette use filter barrier pipette tips and inspect piston frequently For more information visit www hamiltonpipettes com High Vapor Pressure Samples Pipetting solutions with high vapor pressure is not recom mended These liquids may damage the piston and seal Sample Temperature Fluids at a temperature other than that for which the pipette and pipette tips have been calibrated may result in volume measurement errors Storage Store upright in stand Do not lay
6. SoftGrip Single Channel Pipettes User Manual 7 HAMILTON THE MEASURE OF EXCELLENCE 1 of 12 1773 01 Rev E Welcome Thank you for choosing a SoftGrip pipette from the Hamilton Company Before using your new pipette please review the important information in this manual on pipette features basic operation calibration optimizing performance maintenance and troubleshooting For French German and Spanish translations of this manual please visit www hamiltonpipettes com For sales and support in countries outside the U S please contact your local dealer For additional information in the U S call Hamilton Sales and Support at 1 888 525 2123 and visit our website at www hamiltonpipettes com www hamiltonpipettes com Sales Support USA 1 888 525 2123 Pipette Warranty Hamilton Company unconditionally guarantees its products to be free of defects in materials and workmanship for one year from date of delivery Any product that fails due to such defects will be repaired or replaced at our discretion without cost provided the device is returned with an explanation It is the responsibility of the purchaser to determine the suitability of application and material compatibility of the products based on the published specifications of the products The specified accuracy and precision performance of Hamilton pipettes is guaranteed only when used with Hamilton Precision Pipette Tips SoftGrip pipettes are protected
7. any AdvanTip Pipette Safe SoftGrip Soffouch Soffop SoftAide 12 of 12 Document No 1773 01 Rev Hamilton Company 4 08 Printed in U S A
8. by U S Patent Number 5 983 733 with other patents pending 2 of 12 888 525 2123 1773 01 Rev E Hamilton SoftGrip Pipettes Hamilton Company is the leader in precision fluid measurement products We extended our world renowned syringe expertise to the SoftGrip Manual Pipettes specifically designed to deliver maximum accuracy and minimize hand injuries The results set the standard for precision comfort and value SoftGrip pipettes feature a soft nonslip handle for a relaxed grip lighter plunger action for more comfort and a separate tip ejector to reduce hand motion The curved hilt supports the pipette for finer control while pipetting Hardened stainless steel pistons are precision ground and polished to ensure accurate performance and provide exceptional piston and seal life SoftGrip pipettes operate on the air displacement principle and use disposable tips Every pipette is calibrated at the factory with standards traceable to N I S T and shipped with a Certificate of Calibration documenting the pipette s accuracy and precision A complete range of fully autoclavable high performance single and multi channel pipettes are available Two styles of single channel air displacement SoftGrip pipettes are offered fixed volume and adjustable volume Slope Plunger lt a Color Coded Curved Tip l Ejector Button Curved Hilt Mus NW C Volume Display Color Coded Volume Adjus
9. dard method such as DIN 12650 Depending on the frequency of use and the liquids measured other time intervals may be more appropriate Calibration Label and Key Calibration Calibration Plunger Calibration Key Label Reference Cap Showing Mark Vertical lines H 1773 01 Rev E www hamiltonpipettes com 5 of 12 To Calibrate 1 Attach a disposable tip and pre rinse the tip 2 Set the desired or the lowest volume at which the pipette will be used 3 Make 10 measurements of distilled water per tip recording the weights Adjust for environmental factors e g temperature humidity and altitude 4 Calculate the mean and standard deviation 5 If necessary adjust the volume However adjust the volume only after confirming the error is not due to other causes Refer to the sections on Optimizing Performance and Troubleshooting a Remove the calibration label and locate the calibration reference mark b Remove the plunger cap by pulling the plunger button off the pipette c Inset the calibration key turning the calibration key clockwise to increase volume and counter clockwise to decrease volume Each vertical line on the calibration key is approximately 0 2 of the pipette volume d Remove the calibration key and replace the plunger cap Repeat the gravimetric procedure until results are satisfactory 6 Initial and date a new calibration label placing the label on the pipette hilt The pipette can be recalibrated f
10. ip directed into a safe receptacle depress the tip ejector to dispose of the tip Pre Rinsing Tips Pre rinsing pipette tips is recommended for all pipetting operations To pre rinse aspirate and dispense the sample one to three cycles On the last rinse press the plunger past the blowout stop to clear the tip of any sample Make sure there are no micro droplets in the tip before aspirating the first sample If micro droplets remain in the tip replace the tip and pre rinse again Pipette Calibration Hamilton SoftGrip pipettes can be gravimetrically calibrated by the user A universal calibration key is available so that the pipette can be adjusted to accommodate specific fluid viscosities and environmental factors such as temperature humidity altitude etc Use a 5 or 6 place balance depending on the volume being measured A 5 place balance should be accurate to within 0 01 mg and a 6 place balance should be accurate to within a 0 001 mg All items used to perform the calibration must be allowed to equilibrate to room temperature 20 25 C constant to 0 5 C Each time you recalibrate the pipette initial and date a new calibration label and replace the current label Also in accordance with your organization s instrument operation practices and standards we recommend following good laboratory practices by calibrating your pipette on a regular basis i e three month intervals using the following procedure or a known stan
11. or liquids with a density other than water Follow the calibration procedure above with the pipette set at the required volume Use the liquid to be sampled With this type of calibration adjustment the pipette is calibrated only for the liquid used and the volume tested The pipette should be labeled as such and should not be used for other sampling applications unless it is recalibrated Performance Specifications Fixed Volume Pipettes At 100 of Pipette Volume Accuracy Precision Volume Color within within Part No Range Coding 96 96 55019 01 5 uL Steel Blue 1 40 0 75 55019 03 10 uL Purple 0 80 0 40 55019 05 25 uL Forest Green 0 80 0 30 55019 07 50 uL Sandstone 0 60 0 30 55019 09 100 uL Violet 0 50 0 20 55019 11 200 uL Mustard 0 40 0 18 55019 13 250 uL Burnt Orange 0 40 0 18 55019 15 300 uL Brick Red 0 40 0 18 55019 17 500 uL Olive Green 0 40 0 18 55019 19 1 mL Sky Blue 0 30 0 12 Adjustable Volume Pipettes At 10 of At 100 6 of Pipette Volume Pipette Volume Accuracy Precision Accuracy Precision Volume Color within within within within PartNo Range Coding 96 55019 30 0 2 2 uL Aqua 8 00 4 00 1 20 0 60 55019 32 1 10 uL Purple 2 50 1 20 0 80 0 40 55019 34 2 5 25 uL Forest Green 4 50 1 50 0 80 0 20 55019 36 10 100 uL Violet 1 80 0 70 0 60 0 15 55019 38 30 300 uL Brick Red 1 20 0 40 0 40 0 15 55019 40 0 100 1 mL Sky Blue 1 60 0 50 0 30 0 12 6 of 12 888 525 21
12. plunger release Calibration required Calibrate the pipette Seal leak dripping fluid Replace the seal Imprecise Results Inconsistent sampling See section on Optimizing method Performance Seal leak Replace the seal Autoclaving The entire pipette is autoclavable at 121 C for 15 minutes at 15 psi 103 5 kPa or 1 02 atm pressure without the need for any disassembly However make sure to loosen the lower body assembly by 1 Sliding the ejector sleeve off the pipette to access the lower body assembly refer to Pipette Parts Diagrams 2 Loosen the lower body assembly near the adjustment ring Do not completely remove the assembly refer to Pipette Parts Diagrams 3 Slide the ejector sleeve back over the assembly but do not reconnect it It should be loose enough that it slides off if the pipette is turned tip side down Once autoclaved retighten the lower body assembly Although autoclaving should not affect pipette accuracy practice good laboratory procedures by checking your pipette and recalibrate as needed Replacement Parts Hamilton pipettes have been designed so simple repairs and part replace ments can be performed by the user The Replacement Parts Table lists all pipette replacement parts identified by Hamilton part numbers There are three different assembly configurations for SoftGrip pipettes Refer to the Pipette Parts Diagrams for the pipette that needs repair To replace parts slide the ejector sleeve off the lowe
13. r body assembly of the pipette Unscrew the lower body assembly All parts that are replaceable will now slide off the pipette shaft Once the part is replaced reassemble the pipette in the exact order as indicated in the diagram Make sure that the seal flange is oriented towards the plunger with the O ring below the flange towards the tip Lightly lubricate the piston Lubricant Part No 5763 01 using a soft lint free cloth Hold the plunger button down to engage the piston into the seal After reattaching the lower body assembly attach the injector sleeve over the lower body C ring When replacing seals in the 2 uL you need only to remove the tip section of the lower body assembly To assure that your instrument meets the original Hamilton Company standards and specifications we strongly suggest that you follow Good Laboratory Practices by recalibrating your pipette after replacing any parts 8 of 12 888 525 2123 1773 01 Rev E Pipette Parts Diagrams 2 uL Pipette Piston Not user replaceable Piston Not user replaceable 8 Seal Kit 7 Seal tube 5 and 10 uL only 5 Seal pring 9 Lower Body Assembly C ring 12 2uLTip 8 Sealspacer 5 Seal spring 6 Spring Spacer 10 Ejector Sleeve Piston Not user replaceable 9 Lower body assembly C ring 1 Plunger Cap 77777777777 pom 6 Ejector Sleeve A 8 SealKit 6 Sealspacer 5 Seal Spring 4 rw 3 Spring
14. tment Ring Soft Nonslip Handle Tip Ejector mw Disposable Pipette Tip 1773 01 Rev E www hamiltonpipettes com 3 of 12 Volume Setting Hamilton SoftGrip Fixed Volume Pipettes Fixed volume air displacement pipettes are ready to use No adjustment or setup is required Hamilton SoftGrip Adjustable Volume Pipettes The isolated volume adjustment ring prevents accidental volume changes To set the volume hold the pipette vertical with the volume display in front of you and turn the volume adjustment ring to the desired volume Set the desired volume to within the pipette s volume range as given below For the greatest attainable accuracy always turn the adjustment ring down to the desired volume If you are starting at a setting lower than the one desired turn the adjustment ring past that volume and then turn back to the desired volume When setting volumes start your settings from the top digit on the volume setting display Full units are expressed in black digits Incremental units the values to right of the decimal point are expressed in red digits and appear below the full units The graduated scale identifies the least significant digit Notes For the 2 uL 10 25 pL and 1 mL pipettes the red digits on the scale represent values to the right of the decimal point For the 100 and 300 uL pipettes only the last digit obtained from the graduated scale represents a value to the right of the decimal point For
15. use them partial or permanent disability during the inspection or repair process When returning products please ensure the items are decontaminated and marked accordingly Furthermore the customer assumes responsibility should the returned product be determined to be hazardous All returns must be accompanied by a pre approved Returned Goods Authorization Number RGA Any return not received without a RGA may be disposed Contact Hamilton Customer Service at 1 888 525 2123 for an RGA Hamilton Precision Pipette Tips Hamilton delivers performance value and choice with two high quality tip families plus multiple formats and packaging options The tip families are low retention low binding tips for maximum sample recovery and standard tips for routine high throughput work Formats include standard non filter extended length hydrophobic filter barrier non sterile pre sterilized and macrovolume Packaging options include bulk bags hinged and lift off racks and stackable racks Hamilton tips are made from chemically resistant virgin polypropylene for excellent clarity and smooth surfaces All Hamilton Pipette tips are certified to be free of RNase DNase and pyrogens Hamilton pipette tips are universal fit For more information visit www hamiltonpipettes com 1773 01 Rev E www hamiltonpipettes com 11 of 12 www hamiltonpipettes com Sales Support USA 1 888 525 2123 TRADEMARKS The following are trademarks of Hamilton Comp
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