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1. There are several low volume plates available from different vendors Sometimes though the measurement spots require the reader to calibrate the path length the distance to the sensor for every spot and that can slow down the acquisition rate To address that challenge Tecan developed the low volume NanoQuant plate with 16 spots and each one he says is exactly the same path length from the sensor and does not need to be calibrated As a result Fejtl says This is great for customers using low volume and for fluorescence labeling Getting smaller cell numbers in microplate readers can also require higher sensitivity or being able to pick up smaller signals Cells can clump in the wells or grow nonhomogenously but you need to be able to read them Fejtl says Some of our new readers increase sensitivity by a factor of twenty with the optimal read function He adds That s a key feature for cell based assays As well volumes decrease and cell based assays diversify the features of microplate readers keep increasing As Fejtl says There s a whole range of readers With a little shopping you can find just the one for you But make sure to evaluate all the features now available because they keep changing offering new ways to automate and enhance the process of analyzing microplates Mike May 1s a freelance writer and editor living in Ohio You may reach him at mike techtyper com
2. using the following In house service department 28 Third party contract 19 Third party time material 16 Instrument manufacturer time material 11 Instrument manufacturer service contract 8 Multivendor service provider 6 Don t know 6 Our department 5 Other 1 Nearly 44 of respondents plan on purchasing cold storage equipment in the next year The budget ranges for these purchases are as follows E Less than 5 000 m 5 000 15 000 Mm 15 000 30 000 m 30 000 3 gt Lab Manager May 2014 ARE YOU IN THE MARKET FOR COLD STORAGE EQUIPMENT Freezers and refrigerators are an integral part of any laboratory that requires temperature controlled storage Maintaining samples at an optimal temperature while in storage is vital for many lab professionals and this makes choosing the right freezer or refrigerator a crucial task While the average kitchen freezer operates at about 20 C laboratory variants have a much wider range of options depending on the storage conditions needed TOP 5 QUESTIONS You Should Ask When Buying Cold Storage Equipment 1 How is the product manufactured Ask about the quality of the materials used and the product life 2 What is the warranty What does it include and for how long Will anything void the warranty 3 How green is the product Ask the company to provide details on energy efficiency and have them relate it to your return on investment e g in four
3. Confocal microscopy has become established to the point where people view it as routine Brinkman cites multiphoton imaging as another routine technique particularly for visualizing in vivo processes The National Institutes of Health BRAIN Brain Research through Advancing Innovative Neurotech nologies Initiative relies heavily on these two methods Interest in fixed tissue imaging has not slackened either according to Brinkman Pittcon 2014 saw the debuts or formal introductions of at least 25 micro scopes most of them suitable for imaging applications for example e EDAX demonstrated its EBSD electron backscatter diffraction for SEM With a focus on analysis of materials with crystalline structures EBSD al lows users to analyze orientation grain morphology material deformation and distinct crystal phases e Carl Zeiss introduced the EVO SEM for materials characterization and quality assurance e Thermo Fisher showed the DRX xi Raman microscope described below e Olympus unveiled its BX63 imaging microscope with full motorized control a unique focusing mechanism and cellSens Dimension software for cell imaging www labmanager com REGISTER NOW www labmanager com celllineexpert Lab Manager SY EXPERTS Barbara Robertson Alison Glaser Barbara Robertson Senior Research Scientist Cellular Systems Group Leads Discovery and Optimization Bristol Myers Squibb Alison Glaser Application
4. FOR ADDITIONAL RESOURCES ON MICROPLATE READERS INCLUDING USEFUL ARTICLES AND A LIST OF MANUFACTURERS VISIT WWW LABMANAGER COM MICROPLATE TECH 48 Lab Manager May 2014 www labmanager com Any Wavelength Anything Is Possible Any Bandwidth Any Assay CLA RIOuar A CLARIOstar High Performance Microplate Reader with Advanced LVF Monochromators Spectrometer and Filters Advanced Detection for Fluorescence and Luminescence Assays AA e comas te at Set monochromator by typing in center wavelength and bandpass under user defined setting or choose your fluorophore from list You can also drag amp drop the elements in the graph Continuously adjustable wavelengths 320 850 nm Ex Em and bandwidths 8 100 nm for excitation and emission Drag amp Drop Monochromator Settings Increased sensitivity over conventional monochromators Use monochromators filters or a combination of both da Integrated fluorophore library for easy wavelength selection 7 Sal Fluorescence and Luminescence spectral scanning CLARIOstar Additional Features Full spectral absorbance with ultra fast UV Vis spectrometer Laser based Alpha Technology The CLARIOstar s monochromator has Fluorescence Intensity FRET Fluorescence Polarization continuously adjustable wavelengths and TRE TR FRET Luminescence and
5. The lab has acquired a host of new equipment over the last few years including a microwave oven a milk analyzer and Is Your Pipette Leaking 2 Lab tech Deanne Togstad testing samples in microbiology lab a Babcock centrifuge and Labelle hopes that he will be able to update the lab with the next budget Many things have changed in the lab over the years but one thing that does not change often is the staff Ivan Bartakovic science writer LabX Media Group can be reached at tbartakovic labx com or by phone at 888 781 0328 ext 295 MAIN TECHNOLOGIES USED e Combustion protein analyzer e Lactoscope e Microwave moisture analyzer e Chloride analyzer e Muffle furnace e Babcock method e Forced air oven e Moisture balance e Charm test reader BRANDIECH SCIENTIFIC INC Toll Free 888 522 2726 www brandtech com e Validate pipette function in seconds e Works with all pipettes D _ D HE E 00 0 al 0 aD e a Scan QR code for a demonstration A Sa Pie F rt bs AA pja Ad uN Pa ect y Ne t p i pi a i Hari i 5 ofthe i i i i nt Md i Hi ari ee Oe a AE l f Hi k N a m Ay k b oe a ate ae p Ti NAL 62 A BX63 Automated Fluorescence Microscope Olympus www olympus com Chemical MAPRINQ tissues and cells has become the leading edge of microscale imaging Lab Manager May 2014 MAKING SMALL THING
6. specialist knowledge team leaders should continually reconstitute the small groups Breaking through the language barrier The small group concept has particular value when some members of the main group are less fluent than most in the group s ingua franca In some circumstances the team leader can organize small common language groups to brainstorm problems and have the most fluent member of the subgroup report back Alternatively Brett and Janssens wrote the leader can encourage team members to speak in their own native language and have other team members collectively translate While that strategy requires bilingual team members and may be cum bersome in large teams with several different native language speakers the pair adds it has the very nice secondary effect of making the task of passing the language hurdle a team task not an individual task Brett summarizes the strength of the fusion concept with a simple example Look at greeting behavior she says Kissing bowing and shaking hands all achieve greeting effectively In fusion team members realize there s a different way of doing things over there and how the team can use those differences to be more creative Peter Gwynne is a freelance science writer based in Cape Cod Massachusetts He can be reached at pgwynne767 aol com This article first appeared in the January February 2009 edition of the Research Technology Management RTM Journal It is reprinte
7. the ACD Spectrus Platform Q How did you go about defining the success criteria for what you were looking for A You have to speak to internal customers to find out what is required now and then you need a crystal ball to see if this solution can grow to fulfill your requirements in the years fo come One of the first criteria we had was ease of put ting data in If putting data into the database is www labmanager com ASK THE EXPERT Steve Thomas has a degree in chemistry from Warwick University UK Always intrigued by puzzles he gravitated to analytical chemistry choosing a third year project in mass spectrometry under Prof Keith Jennings Steve graduated in 1990 taking a position in the NMR department of Merck s Neuroscience Research Centre at Terlings Park While gaining a wealth of experience in medicinal chemistry support he became analytically bilingual speaking both NMR and mass spec to tackle the most challenging aspect of the role the structural identification of drug metabolites He expanded on this role leaving Merck in 2006 for GSK to join the Biotransformation and Drug Disposition group as an investigator within the Drug Metabolism and Pharmacokinetics department at Ware in the UK The seamless combination of analytical techniques to generate reliable definitive structures was even more vital as he was moving from a discovery to a development environment To facilitate assignments and add confidence to results ready a
8. 5 C above ambient to 350 C b ngubator tem d b Gravity forced convection vacuum and drying ovens Standard and refrigerated i incubators Directions here www binder incubator us www binder oven us BINDER BUSINESS MANAGEMENT and see 1f that person has problems locating or navigating your website Make sure specific keywords bring your website to the front when lab services are searched for Check that contact information for your lab is readily located Lastly feel free to include pictures videos and articles that describe your lab and services Consider developing pamphlets or brochures that address specific con cerns that private citizens and small businesses may have Provide sugges tions for selecting tests on the basis of specific circumstances for instance age of home plumbing potential of farm industrial runoff to groundwa ter For water quality or safety issues discuss the importance of regular testing and how your lab can be of service There are many different ways to get these brochures into the hands of these potential clients Consider developing pamphlets or brochures that address specific concerns that private citizens and small businesses may have Contact local dentists doctors and hospitals about your testing servic es Ask if you may drop off a packet explaining your testing options If they have their own contract lab that handles their work don t be afraid to ask
9. Because of its mild ionization mechanism MALDI matrix assisted laser desorption 1onization has been the mass spectrometry imaging technique of choice for years All major MS companies sell MALDI systems Both com mercial and public domain image software exists as well Spiral TOF A Mass Spec Imaging Services LAESI MSI Protea Biosciences https proteabt0 com A Spiral TOF MALDI TOF TOF System FEOL wwwyeolusa com Lab Manager May 2014 MALDI imaging takes place on thin tissue samples where the mass of one or more target molecules is known With assistance from software MS creates 2 D distribution images of drugs natural products or me tabolites along a tissue cross section The sample 1s first treated with a matrix solution that assists 1n volatiliza tion and ionization Then a laser focused on tiny pixels vaporizes a bit of sample which is swept into the spec trometer Software then creates a 2 D distribution image for the molecule of interest MALDI may be combined with other imaging meth ods for example magnetic resonance or by taking successive tissue slices in order to create 3 D images MS imaging of large complex molecules would not be pos sible without gentle ionization Otherwise ionization would destroy or disrupt proteins and other large labile molecules An emerging MS imaging platform LAESI laser assisted electrospray ionization is based on mild electrospray ioniza tion ESI
10. built on and fully integrated with SampleManager LIMS allowing LES functionality to be accessed from the LIMS or from any web browser Combined with the instrument integration capabilities of Integration Manager and the raw data storage and retrieval capabilities of our SDMS Data Manager the new LES expands our growing informatics platform and offers you the most comprehensive paperless lab solution available today SampleManager 11 LIMS with Lab Execution System e thermoscientific com LES SampleManager LIMS at a glance e Method and SOP Execution e Scientific Data Management e Workflow Design e Lifecycle Workflow e Instrument Integration e Dashboards Manage Sama soluto Y KO i amp a O gt 3 S o 2 So e O AE O dE LL o Qe E D EO oN O i o C 2 tit So oF SS OFE Introducing the VersaCool Refrigerated Circulating Bath We started with the form factor how to gain a large work area with a limited footprint As with most great innovations the solution was simple we removed the control head to create more space We lost our head so you don t lose your cool e thermoscientific com versacool D eadless help me go SCIENTIFIC BPA AAA Lab Manager Magazine is audited by BPA eppendorf PhysioCare _ ERA p rn E ee E ee EE EE EE A A A o AA A AA A O O II E A E o a E E 49 3 y M E
11. deep x 11 4 wide x 21 3 high Lightweight Take it from lab to lab when sharing is a must Simple elegance It s easy to install Just plug and play Learn more at wwwi lal or call Protecting your laboratory environment LABCONCO Labconco Corporation Kansas City MO 800 821 5525 www labconco com MAINTENANCE MATTERS CENTRIFUGES DAILY CLEANING AND INSPECTION KEEP THINGS SPINNING s you can imagine with a piece of lab equipment that spins at high speeds maintenance is important not just to keep a centrifuge running properly but also to prevent accidents Wiping the centrifuge down after each use to prevent contamination ensuring there 1s enough space around the unit for proper venting and looking after the rotor are especially critical according to manufacturers It s extremely important that the user keeps an eye on the status of the rotor maintains it properly cleans it and prevents aggressive chemicals from getting in says Maurizio Merli global product manager at Thermo Fisher Scientific A rotor which is not properly main tained is a rotor that could explode and can cause dam age to the unit and potentially to the people around He adds this only applies to metal rotors carbon fiber rotors for example are maintenance free as they are designed to basically last forever WHAT TO KNOW BEFORE SIGNING UP FOR A SERVICE AGREEMENT e Is coverage on site or off site How
12. depending on what is being kept track of In the lab we use standard laboratory notebooks to record experimental details Matosevic says We also use digital tools such as file sharing systems as well as networked drives to keep a record of laboratory in formation such as raw data images and files When it comes to regulatory documentation we do it exclusively using computers While such software and digital tools make documen tation easier they do pose some challenges to researchers and managers Matosevic says one of the biggest issues the lab at Akron faces is standardizing laboratory record keeping across the spectrum of its laboratory operations and finding a digital platform that will let workers main tain the lab more efficiently than they ve been able to do with lab notebooks This relates to keeping track of laboratory operations as our lab grows and maintaining the same level of control as mul tiple scientists work on multiple projects he says I think the key is to have consistency in the way multiple users record their data We work through this by training and evaluating our laboratory personnel in efficient and thorough record keeping Currently Akron is evaluating LabArchives and simi lar electronic platforms to determine how well research ers needs will be handled in terms of digital file storage note taking data analysis etc Schwartz also faces some technology related chal
13. information then it can cause their work to come into question No good scientist wants to be in that situation and management emphasizes this aspect For Schwartz s lab maintaining an air of transparency 1s the key to ensuring that staff members are documenting everything By having complete trans parency accountability follows because if you re not doing your job and everything s transparent everyone knows it he explains adding that his lab has staff meetings once a week to discuss in detail what everyone s doing and how they are doing it Along with training in proper lab record keeping Akron also has regular meetings and checks from lab management to ensure that documenta tion is always proper and up to date Preparation of batch records for regulated processes is always done from raw data i e the contents of the lab notebook which makes laboratory personnel aware of the importance of keeping the lab record up to date Matosevic adds For those new to documentation in the cell culture world proper planning is essential to avoid a potential mess Assess your needs and never forget about regulations Matosevic advises If you are starting out consider a digital e notebook platform as it allows you to keep a close eye on every user in the lab and address issues promptly Train personnel in record keeping and ensure they follow standards Zaidi adds that scientists should alw
14. lenges in his lab mostly dealing with software updates or having to switch to a new operating system For us Windows XP is going away so we have to go to Windows 7 and now the software doesn t work on Windows 7 he says There s always a struggle with keeping the software up to date and keeping 1t communi cating with the operating system without having to buy this stuff over and over again However he says the main challenge for his lab is simply making sure documentation 1s being done and done properly The biggest challenge really is to make sure that your staff is adequately trained to keep track of the doc umentation in the appropriate manner and understand the importance of doing so Schwartz says Ultimately Lab Manager Moy 2014 If we did not keep accurate documentation w ed be stumbling around in the dark when things went wrong all of this depends on somebody doing something If somebody doesn t do that something it doesn t get done and the system fails Making sure it s done Ensuring everyone consistently captures all pertinent information is also the main challenge in Zaidi s lab one that Stamford Bioprocess tackles through proper training and having staff review each other s work she says Our scientists know the importance of document ing the work as we will often be asked about a particular detail of the project If they don t have the requested
15. 10x magnification Gen5 software s image analysis capability allows the images to be processed in many ways providing greater detail on how diclofenac affects the spheroid The mean green fluorescence in the field of view can be plotted Figure 1B providing a relative impression of increasing toxicity based on the disruption of the plasma membranes of the cells comprising the spheroid A fluorescence ratio of green dead cell fluorescence to blue total cell fluorescence can also be plotted which yields a different profile at higher diclofenac concentration These data are equivalent to that obtainable from PMT based optics but with improved S B which suggests that diclofenac toxicity reaches a maximum at 30 uM Ratiometric data is rather confusing as it suggests that diclofenac toxicity is inexplicably reduced at higher concentration Upon examining the spheroid images in Figure 1A it is evident that the spheroids are expanding with increasing diclofenac concentration Gen5 can use image analysis routines to compute spheroid size based on a diameter and spheroid area which 1s plotted in Figure 1C Quantitative data indicate that the spheroids do increase in size up to 30 uM diclofenac but at the highest dose it appears the integrity of the spheroid is partially ruptured and the size and area is reduced These data suggest an explanation for the ratiometric data seen in Figure 1B Finally cell counting in a focal plane that bisects the spher
16. 41 Annual Conference amp Trade Show Indianapolis IN July 27 August 1 2014 WHO ARE WE The National Association of Scientific Materials Managers NAOSMM is a professional organiza tion of approximately 500 individuals in the USA and beyond involved in purchasing inventory management and control and the safety and regulatory matters of laboratory chemicals supplies instrumentation and special services in academia research and industry With nearly 200 million dollars in buying power members receive excellent discounts from many vendors Join with us to help you run an efficient stockroom provide great customer service understand federal and local regulations and share best practices in running your laboratory and stockrooms National Association of Scientific Materials Managers Supporting the professional development of scientific laboratory amp materials managers JOIN US AT OUR 41ST ANNUAL CONFERENCE AND TRADE SHOW JW MARRIOTT INDIANAPOLIS IN Sunday July 27 Friday August 1 2014 YOU CAN T AFFORT NOT TO ATTEND THIS YEAR S CONFERENCE Over two full days of education and professional development to help you save time money and help you enhance your career Two half days exclusively for trade show and vendor interaction Network with peers in academia research and industry Training a gears by the Laboratory Safety Institute and much more FOR MORE INFORMATION PLEASE VISIT
17. A7 Peer MULTIPLATFORM 47 eee OER o Completed Surveys 270 SURVEY SAYS Types of ELN installations used by survey respondents Web based 38 Stand alone 14 Thin client server 1 Client Server 37 Other 10 Primary purpose for ELNs as reported by survey respondents Centralized data repositories 8 Infrastructure for capturing accessing o 21 and sharing experimental information Improved communication between instruments 4 and related software Accelerating the documentation and reporting 3 i 15 of experimentation Enabling scientists to collaborate effectively gt 10 on multi stage projects Workflow coordination across geographic 3 and business boundaries Streamlined regulatory compliance 1 Intellectual property IP protection 6 Patent evidence creation 0 Improve productivity 4 All of the above 27 Nearly 56 of respondents plan on purchasing an ELN in the next year The reasons for these purchases are as follows Infrastructure for capturing accessing and sharing 19 experimental information Accelerating the documentation and reporting 7 16 of experimentation Setting up a new lab 12 Centralized data repositories 12 Enabling scientists to collaborate effectively 8 on multi stage projects Improved communication between instruments 7 and related software Upgrading existing ELNs 6 Addition to existing systems increase capacity 6 Streamlined regulatory compliance 4 Web based
18. BRET bandwidths for excitation and emission i ad giving it filter like performance Reagent injectors for kinetic or cell based assays Low volume DNA measurements www bmglabtech com BMG LABTECH Australia France Germany Japan United Kingdom United States The Microplate Reader Company Alpha Technology is a registered trademark of PerkinElmer Inc HTRF is a registered trademark of Cisbio Bioassays 50 PRODUCT FOCUS MASS SPECTROMETERS MASS SPECTROMETERS Lab Manager GROWTH FUELED BY LIFE SCIENCES MINIATURIZATION by Angelo DePalma PhD ccording to a report from Persistence Market A Research Mass Spectrometry Technologies Market Assessment Report Global Size Share Competitive Landscape Future Prospects and Forecast from 2013 2017 mass spectrometry MS 1s one of the few instrumentation markets that has recovered fully from the last recession The study estimated the total market for MS at 3 175 billion in 2012 and expects it to reach 4 84 billion by 2017 a growth rate of 8 8 percent per year North America and Europe comprise more than half of current demand 32 percent and 29 percent respectively with Asia Pacific close behind at 27 percent Top players are AB Sciex Thermo Fisher Scientific Agilent Waters Bruker and Shimadzu Growth in life science markets particularly in pharmaceuticals and biotech are fueling the growth in MS The report specifically cites miniaturization as a
19. Convention Center in Baltimore Maryland This dynamic scientific conference features a variety of sessions and short courses and will close with an event at the National Aquarium Please note that the products shown here may not be at the show but their manufacturers will be Raman Microscope XploRA PLUS Incorporates unique and powerful research functions in an impressively compact analytical bench footprint Is a fully confocal and high performance Raman microscope Offers an unmatched and enhanced range of options such as multiple laser as complete automation EMCCD detection Raman polarization and even AFM coupling The system s SWIFT Fast Raman imaging is typically 10x faster than conventional mapping methods HORIBA www horiba com scientific MALDI TOF TOF System Updates NS SpiralTOF Mi BOOTH 107 e Resolving power specification has now been increased to 75 000 e JEOL now offers MSVision software from PREMIER Biosoft for processing the large data sets from MALDI imaging of ultra high resolution MS data e Unique ion optics with a 17 meter flight tube in a compact package give high MALDI TOF resolving power and monoisotopic precursor selection for high energy CID to probe chemical structure JEOL www jeolusa com Spectrometer Maya LSL Delivers excellent stray light performance with high sensitivity Provides high sensitivity high throughput performance for applications ranging from chemical catalysis fo Raman anal
20. E DE o se IS E E E E E A O O PA ee ee U A A A A A E A A A E CI A E 3 E ee E E E E o CI SE A E E A A A E E O A A A A O O O A A A A O A O e e CE A E AA E A A A E E E E E E E E E O CHE EE eoeoeeeee ee ees5gqeeee e eeee esks 8e 8 T E en e k E A E A E o M E E E E E E A A E J E E E E E E E E O A E E A A A A A O A A 2 F E E SE E E E o 0 2 LE Sl E E A E E E E A z 000 L LA lt I A E S SI E E E E E E A A A A A A A A O O E E E A a 4 a E A A E A A E E A A A A A A A A A A ee CEE E E E A A A A A A O A A A A A A A A A A A A A A A A A A A A A A A A A A o CN E E E A A O A A A A A A A A A O TAC E E A E A A A O A A A A A A A A A A A A A A A A A O A A A CC E E E E E E E E 0E A 6 O O hee A A A A A A O A A O E O E A A O A E O O E E A CUCM HChUCM OC A O O A A O O O E E A A A E O O E A E EE E A E A o LEE E E E E E E Ss e eeeeeemmeese eerceceeeeeee eeee eee03qneeee e ee eeee ee e eeeeerestee CA A E E CS SE ee A A ADS A SL A A A A A A A A A A A A A A A A E The new Reference 2 boasts gt 1 button operation ergonomic proven Premium characteristics handling reduces operating effort with innovative technology for gt High precision and accuracy for extraordinary precision and accuracy reliable pipetting results a long service life and an ergonomic gt Volume lock prevents accidental design making ita reliable lab partner changes for you and your demanding work gt RFID chip contains pipette data www eppendo
21. Scientist Biotherapeutics Molecular Devices SPONSORED BY Molecular e9 Devices www moleculardevices com ASK THE EXPERT WebcastSeries WEDNESDAY MAY 21 2014 12 00 1 00 PM EDT There is a growing need within drug discovery to improve efficiencies in cell line development Specifically development timelines need to be shortened suc cess rates for clone identification need to be improved and overall productivity needs to increase to meet the growing demand for new cell lines To enhance efficiency and improve flexibility of the process we have replaced the dilution cloning method with automated clone picking using the ClonePix 2 System for the majority of the cell line development efforts The ClonePix 2 System couples intelligent image analysis with automated colony picking from semi solid media enabling rapid hands off screening through thousands of clones expressing sUr face proteins and clone picking based on rate of growth and expression levels During this webinar you will learn how to e Identify clones with varying levels of target expression using antibodies directed to protein extracellular epitopes and epitope tags e Use fluorescent ligands and labeled small molecules to identify clones with surface expression of targets of interest e Reduce cell line development timeline and tailor design of new cell reagents using image based clone selection as opposed to dilution cloning REGISTER NOW www la
22. Skip the Prep Go Direct AGILENT I AB SCIEX 9979 1890 2 3200 OTRAP Triple Quad Performance VSpravES Shimadzu Prominence pray Front End Available GC MS Many systems amp configurations available THERM10 a THERMO E ORBITRAP Abd LY J gt VELOS PRO Benchtop LC MS iE 2 THERMO ASK ABOUT TSQ Vantage 1 YEAR WARRANTY e RENT LEASE BUY with HESI Probe Installation Method Set Up Chemist Support Training WA TERS 20 4000 LC MS Installation amp One Year Warranty Available 150 QUANTUM ACCESS Mass range 3000 amu www gentechscientific ccom sales gentechscientific com 585 492 1068 Roles and responsibilities The employees in the lab have a variety of experiences and degrees and although a degree 1s not required to work in the lab some employees have bachelors degrees in microbiology or dairy technology The technicians receive training beyond basic lab responsibilities and techniques as they undergo HACCP Hazard Analysis Critical Control Point GMP Good Manufacturing Practice and safety training along with a month of hands on training in the lab before they are left to work independently The technicians also obtain water and antibiotic testing certification and licenses since the lab at Agropur must meet the requirements of a certified lab Labelle has experience in research and development and quality assurance and has worked as a lab
23. So we wanted to push the software further than it was intended We did a pilot trial and the software held up giv ing us the possibility to store the data with a bio transformation map which included a schematic of the complete metabolic fate of a drug as a top level executive summary So the people who were interested in just knowing what happens to the drug in our body could look at the top level summary and people who were more interested in the analysis to prove those structures could dig down deeper in the database One of the things that became apparent was that this database could be searched from vari ous angles As you associate data with a mol ecule you are building up metadata as well as an information rich environment around its basic structure People were interested in this data for many reasons Some wanted the NMR data to help with their analysis while others needed the fragmentation data trom MS or wanted to know which liver enzyme caused this metabolite to be produced So what people really wanted was a data cube a database that could be picked up and turned around to look at the facet that you were interested in They wanted something that could be drilled down trom the angle or perspective that you asked your question trom So that s how we started using ACD ChemFolder Enterprise which has now morphed into a single integrated chemical and analytical Knowledge management solution on their latest offering
24. Takeaways from this month s issue PARTING POINTS Takeaways from this month s issue GOOD CHEMISTRY With expanding roles in helping design experiments collect samples replenish consumables monitor projects access literature overcome software limitations and collate data along with many other functions apps are fast becoming indispensable in the laboratory by e Improving personal efficiency and group coordination e Helping make gathering data easier e Boosting security and compliance e Making instrument operation and access simpler MARKETING YOUR LAB MANAGING CULTURALLY As budgets for agencies tighten available grant money shrinks and D IVE RSE TEAMS money for lab testing becomes less of a priority it is increasingly Managers of R amp D groups in North America and elsewhere face multi important for labs to draw in new business to increase revenue Labs cultural situations with increasing frequency In those situations they must can do this by deal with the potential for multiple cultural clashes among team members e Identifying who their clients are where they re located and what they need The fusion approach is one way to solve such problems It allows e Using a multifaceted approach in their marketing plan e Coexistence of differences e Ensuring they have an easy to use website e Meaningful participation from all team members e Engaging with their communities by speaking at local events etc e Crea
25. a boon to lab staffers because it increases flexibility facili tates movement between work stations and across functional areas and enables related functions such as accessing references and stan dards all without interrupting the primary work flow An app with precisely this in mind was released by Grand Interactive which develops customized mobile applications for use with instrumentation in the biotech nology and life science areas Designed to obtain real time performance data from the ProFlex PCR System from Life Technologies the PCR Essentials app allows lab personnel using 10S and Android based mobile devices to monitor review and share data as well as to perform other tasks remotely The app also provides instant access to master mix calculators and pertinent how to videos www labmanager com ECKMAN COULTER Life Scien i ces Avanti JXN me Running 4 000 at Spe iach 14 000 RPM Y 0 09 Set Time 075 hmm 20 Sa p Sel Temp 25 G Accel M x Decel y Max gt START Discover more at www beckmancoultercentrifugation com THE NEW AVANTI JXN IHE FREEDOM OF ANYWHERE Already the leader in high performance centrifuges Avanti is yet again advancing your research and simplifying your workflow With an enhanced LCD touch screen plus anywhere anytime mobile access with MobileFuge youre in control in the lab and out oulter Inc 12 GOOD CHEMISTRY The typica
26. adds More and more people in research labs are working with live cells in microplates In many cases a life scientist selects the live approach because 1t replicates biology in more realistic conditions To maintain healthy conditions for live cells some microplate readers now control the sample environment for carbon dioxide and oxygen Live cells often need other pampering such as shaking them in just the right way So scientists can select a microplate reader that provides a range of shaking options As an example Amouretti says Yeast tend to grow when in suspension so you need a reader that shakes continuously while you make the measurement In addition the surge in cell based assays spawned other advances in microplate readers For instance some vendors now make a microplate reader that includes a microscope So you can make a measurement in a well and image the cells says Amouretti In Salvatore Pizzo s laboratory at Duke University in Durham North Carolina for example lab research analyst Maggie Kennedy uses the BioTek Cytation 3 May 2014 plate reader mainly for cell based assays She says I look for versatility in a plate reader something that can perform multiple functions in one machine She also appreciates that this microplate reader allows more than one mode of detection She says I love the microscope function Being able to visualize individual cells in addition to getting
27. award winning technology also involved a collaboration Texas Instruments TI DLP NIRscan evaluation module for spectroscopy which 1s built by Keynote Photonics won gold in the 2014 Editors Choice awards The company also announced the first DLP device optimized for use with near infrared light on Mar 3 With these technologies TI hopes to enable spectrometers for use in the food pharmaceutical oil and gas and emerging industries to deliver lab quality performance in the field and manufacturing environments We continue to push the limits of what DLP technology is capable of opening it up for use in advanced scientific industrial and medical solu tions said Mariquita Gordon DLP Embedded manager at TT Two other manufacturers that will be teaming up are Metrohm USA and Waters Inc Waters will now develop and release Empower chromatography data system CDS drivers for Me trohm s new 900 series ion chromato graphs and its older 800 series modules We are excited to be able to provide Waters Empower customers with access to Metrohm s innovative IC technology said Edward Colihan president of Metrohm USA The ability to have instrument control and data processing within a single CDS is of paramount importance to the pharmaceutical customer Specific modules of Metrohm s new Flex and Vario 10n chromatographs are set to be released later in 2014 Bio Rad Laboratories had its ow
28. be unsafe to drink Pass out or place coupons in programs for discounted testing Lab Manager May 2014 www labmanager com PEVegereriry REQUGGUOCLS tii FreezerPro was designed purely for sample management This focus allows it to eliminate wasteful inefficiencies and help streamline laboratory production without the resource requirements associated with adopting a LIMS FreezerPro enhances user s ability to work collaborate and learn through traceability automati on congu rable re eporting HEET nication to ools and hardy are support For thou ds of use worldwide E Free ze Pro ISL abor atory il Informe ation B BIISS Mas pae BUSINESS MANAGEMENT Libraries and schools often have display areas or allow Contact public offices assessors clerk offices city hall the posting of presentations to the general public Check etc to inquire about their display cases or areas that you to see if you can take advantage of this low cost op may be able to utilize to draw attention to your lab Also tion for promoting your lab services while educating the many municipalities have bill stuffers with annual tax public on matters of local interest statements or utility bills This may be another opportu nity to advertise your lab services If a higher percentage of your samples come from businesses contracted clients researchers with grants etc a different approach is needed You will need to find the best way to reac
29. bottom reading and Optimal Read OR www tecan com readerfamily GTECAN Call The Americas 1 919 3615200 Europe 49 79 5194 170 Asia 81 44 556 7311 info tecan com 2014 Tecan Trading AG Switzerland all rights reserved For disclaimer and trademarks please visit www tecan com Panasonic Life Science celliQ INTELLIGENT COQ INCUBATOR Smart TOUCH SCREEN INTERFACE 2 min CO2 RECOVERY A 8x Faster Rethink cell growth Empower your cell culture through the most advanced user Discve ry powered by interface and technology Optimize your environment with a new Intelligent touch screen for uniform temperature control p Pecision precise CO recovery and rapid decontamination Learn more at us panasonic healthcare com celliqseries 4 www labmanager com May 2014 Lab Mana Run Your Lab Like a Business Good Chemistry Mobile apps have become a common part of everyday life With expanding roles in helping design experiments collect samples replenish consumables monitor projects access literature overcome software limitations and collate data along with many other functions apps are fast becoming indispensable in the laboratory also Bernard Tulsi Perspective On A Food and Beverage Lab Situated in rural Weyauwega Wisconsin the Agropur Dairy Cooperative plant continues the rich cheese making tradition of the state through its production of both cheddar and feta ch
30. bring business Make sure that when staff are quoted or write articles your laboratory name is included When your staff become known as experts on topics people are more likely to use your lab when they need testing Marketing your lab can be accomplished with a little elbow grease and some legwork often at little cost By doing research up front on potential customers and planning your marketing based on the targeted audience you will be more successful reaching your customers with the right message I would like to thank my coworkers and supervisor at PHMDC for their support ideas input and feedback Lynda Seeger Environmental Chemist Public Health Madison amp Dane County Madison Wisconsin can be reached at LSeeger publichealthmdc com Viscometry at its Best gt No glass ware or water bath gt Accepted in the ASTM Standard Specification for Diesel Fuel gt Up to 4 times faster than Test Method D445 gt Temp Range 56 to 105 C with temperature control 005 C gt Equivalent to results from Test Method D445 5 Anton P nion raar Anton Paar USA Tel 1 804 550 1051 info us anton paar com www anton paar com WhatsAPNews 26 LEADERSHIP 8 STAFFING k N da _ md MANAGING CULTURALLY DIVERSE TEAMS by Peter Gwynne hen French company Groupe Bull prepared to merge with American firm Zenith Data Systems American and French engineers working for Bull discussed the di
31. but has several advantages over MALDI For one LAESI essentially eliminates matrix addition and more significantly sample prep Matrix addition can create artifacts that can be bypassed by eliminat ing sample prep entirely observes Haddon Goodman LAESI platform marketing manager at Protea Biosci ences Morgantown WV Unlike imaging methods that require contrast agents or labeling with radioactive compounds and sophisticated image acquisition instrumentation molecular imag ing exemplified by MALDI and LAESI relies only on the masses of target analytes which are filtered by the spectrometer LAESI can create multiple chemical maps of a surface simply by filtering a panel of masses The critical difference between molecular imaging and say MRI is that the latter usually takes place in living organisms whereas MS methods cannot You need a slice of tissue Goodman says Where both LAESI and MALDI create 3 D images through sectioning LAESI alone is capable of tunnel ing through samples from point to point This technique has been demonstrated for plant tissues where cell walls maintain structural integrity Goodman says his company is working on a similar method for animal tissue LAESI s most interesting imaging strength has to do with rapid screening of surfaces without sample prep In addition to plant and animal tissue LAESI is capable of scanning surfaces of cultured cells in plates or Petri d
32. content from experts in the field to discover the best solution for their research needs Events Event Sponsors eppendorf Presentation Is Social Media the Future of Science serail AirClean Systems Forms Contra Espert F cking Love Science IFLS Facebook page Maver ix Lab Design and Furnishings Jennifer Webb private architectural consultant It s All About the Data What it Takes to Get It Manage It and Use It in a Modern Laboratory Joe Liscouski a Executive Director Additional SR amp LS Events Institute for Laboratory Automation Webinars New Frontiers in Next Generation Sequencing Effective Chemical Managemen ective Chemical Management e Search for Novel Noninvasive Biomarkers for Vince McLeod Companion Diagnostics Senior Industrial Hygienist University of Florida Environmental Health and Safety Division Online Auction LabX is pleased to present the biggest one day auction in our company s 19 year history Best Practices of Purchasing Lab Equipment Manufacturer direct surplus and demo equipment Mike May PhD Bid on items from leading manufacturers including Freelance Writer amp Buchi evaporation equipment Eppendorf North Editor for Science amp Technology America s extensive laboratory product line and Heidolph rotary evaporators shakers and mixers For complete list of speakers and to secure your spot please visit www labmanager com SRLS 72 TECHNOLOGY NEWS M
33. data entry daily The role of the lab supervisor has also changed It used to be that the lab supervisor just supervised the lab and handled the reports Labelle says and now along with the quality assurance manager the two are responsible for approving and releasing the product for shipment The Oracle system assists with the approval process since the system does not allow the product to be released unless standards are met Labelle also works closely with the field staff in other plant locations and although there is a member of staff who performs HACCP record reviews in conjunction with the QA manager Labelle is ultimately responsible for HAACP reviews pertaining to milk coming into the plant Making sure everything goes smoothly is the important thing Labelle says regarding the day to day operation of the lab and facility but there can be some unexpected challenges such as equipment that fails and requires immediate repair while the lab still needs to complete testing Labelle also describes the importance of coordination between production and the laboratory because trucks could be delayed by waiting for the release of the product because testing is not complete Most communication in the lab is face to face and Labelle interacts with staff on all the shifts Notes are left on a lab whiteboard if there is important information to 60 www labmanager com PERSPECTIVE ON A FOOD AND BEVERAGE LAB 2 Lab tec
34. drug screens not only because routine protocols have not been developed tor their detection but also because their association with complex plant matrices during manufacture generally requires laborintensive extraction and sample preparation for analysis To address this new and important challenge in forensic chemistry Direct Analysis in Real Time Mass Spectrometry DART MS is applied to the analysis of these designer drugs GenTech WA 585 492 1068 www GenTechScientific com Lab Manager Moy 2014 Methods DARFMS was employed to sample synthetic cannabinoids directly on botanical matrices The ambient ionization method associated with DARTMS permitted the analysis of solid herbal samples directly without the need for extraction or sample preparation The high mass resolution time oHlight analyzer allowed identification of these substances despite their presence within a complex matrix and enabled difterentiation of closely related analogs Results DARTFMS was performed to rapidly identify the synthetic cannabinoids AM 251 and JWH 015 For each cannabinoid three hundred micro grams 300 yg of material was easily detected within an excess of background matrix by mass Conclusions New variations of herbal blends containing a wide range of base com ponents and laced with synthetic cannabinoids are being produced making their presence difficult to track by conventional methods DART MS permits rapid ident
35. in a variety of sample types For example researchers talk about merely waving samples before a LAESI device and achieving sufficient ionization for MS analysis Says Haddon Goodman LAESI platform marketing manager for Protea Biosciences Morgantown WV LAESI s main advantage is operation at ambient pressure with no sample prep and no addition of matrix Because it occurs at ambient pressure LAESI allows users to analyze bacterial and fungal colonies for interesting natural products that may be channeled into say a drug development pipeline LAESI enables investigators to search through hundreds of thousands of colonies to find specific molecules of interest Goodman tells Lab Manager A group at the University of Oklahoma is screening fungal colonies for secondary metabolites for example LAESI is just about the only way to analyze them at this level of throughput Goodman adds It allows users to submit samples that could previously not be analyzed by mass spec Goodman describes LAESI as a post sample introduction method Unlike LC it does not take heart cuts of peaks And by itself LAESI does nothing t requires an MS to work its magic It s a front end for MS Goodman says The no sample prep aspect of LAESI results from its operation It uses a 2 94 micron laser tuned to the absorption lines of water When the laser strikes the sample it induces rapid boiling by ex
36. je y Font columna in addon tte sample can also be atperaed wah the pipette fom an extemal sources before plang it on he VIAFLO ASSIST amd alareng Rie protocol Pinte Filing Filling mutbp plales mighi mod be a drfbcult tase Sut is kreni none in ceder do improve pipethng Erponomica manual ppeting should e mirimined VIAFLO ASSIST heips pou lo ME micropiates withoul Na my petia strain inpury Reagent Addition Draw reagent rom fhe reagent resarcir a POR tude fing ha Plate foe or an exiernal source lo add 110 he target plate an opbonal mung sep helps to achine a homegencas aMbuton othe tamli Fora precisa liquid Pantset and lo make sure Pal no remaming liquid ginga to the bp end performing a bouch of ys common padie MAFLO ASSIST allows bof a auth of on ie side wH ol he well a made the lkausd INTEGRA A Watch a video http www integra biosciences com sites viaflo_assist html video Hudson NH 03051 Download product brochure http www integra biosciences com sites pdf USA flyer 127951_VO1_Flyer_VIAFLO_Assist_EN pdf Phone 603 578 5800 Website www integra biosciences com Lab Manager May 2014 www labmanager com PRODUCTS IN ACTION How To Protect Clinical Researchers amp Testing Analysts In clinical lab environments as in any laboratory using chemicals work processes pose an inherent risk of hazardous chemical exposure for operators and for the lab environment This problem is made more difficult
37. little doubt that apps increase efficiency You have more information available to you and there 1s a fun factor as well when you can pull up data right on the smartphone Apps provide end users with a number of functional ities right on the phone at their fingertips and most apps are extremely user friendly they are so intuitive there s no need for manuals to tell you how they work Still there is definitely a bit of a generational divide in the use of apps according to Barker We see younger people quickly taking to the SAM 1 at our trade show booth They take instantly to the PH 1 because they do everything on their smartphones these days We hear those comments from the younger crowd all the time Some people in the older crowd use and understand the new technologies also but some have an older more reluctant approach says Barker He notes that some older lab folks still have flip phones or no cell phone at all They look at our product and ask why would I use that I have pen and paper and they do the job for me There is a bit of a divide but still we see increasing numbers of people going for these devices We try to make them as user friendly as possible so that anybody could use them By incorporating features that make jobs easier everyone young and old could quickly derive the benefits Bernard Tulsi is a freelance writer based in Newark Del He may be contacted at btulsi comcast
38. much downtime can the user expect e What is the usual turnaround time on repairs e Is the manufacturer using original parts or used refurbished e What is the limit to the total cost of repairs covered by the agreement e Does the agreement entitle users to any software or other upgrades made to their centrifuge after purchase What is the number and location of trained field service engineers Vendors offer a number of programs from simple preventive maintenance to more intensive agreements to inspection programs like Beckman Coulter s Field Rotor Inspection Program Celebrating 30 years of delivering lA airi to your lab In general keeping it clean every time you use it or at least weekly is important says Randall Lockner market ing manager Americas Centrifugation at Beckman Coul ter Life Sciences Wiping down the interior portion of the centrifuge the rotor chamber and also any of the surfaces that have electronic components such as touchscreens or keypads is a good idea when doing daily cleaning he adds Many users however often fail to clean the chamber Most customers don t really like cleaning the cham ber because it s dirty or because it contains chemicals it contains spills from blood or urine you name it but also because it s difficult to access the chamber because there s a rotor in place Merli explains adding the latest centrifuges have features that make rotor remo
39. net or by phone at 302 266 6420 Use a balance to check a pipette AND A amp D Weighing Ensure your pipettes are accurate and get even more value from your BM Series Microbalance Now get the same accuracy that you would get from sending your pipettes out to a calibration lab Get our latest catalog Laboratory Solutions at www andweighing com 16 SCIENCE MATTERS IPLINARY TEAMS y now we all know that the life sciences industry is radically changing For decades we had been comfortable working within rigid boundaries Within our own little si los we shared space with like minded colleagues people who studied and worked on the same projects Our ideas flowed between one another but rarely outside the confines of our discipline We produced very high quality work But unfortunately we didn t necessar ily know how to relate to others whose work radically or even just slightly differed from ours Se y d e l N d y there on how to do this It s a relatively new phenomenon especially in the life sciences Hopefully managers are working with team members who are competent and intelligent in their ap proach to their daily jobs But getting them to work together effortlessly and synergistically toward the same goal is nevertheless a difficult task Merely bringing everyone together on a project isn t necessarily going to do the trick Meaningful cross functional co
40. one com pact affordable instrument WWith this unique combination Biolek brings microplate detection analysis and automated digital microscopy to researchers without the need for separate expensive and complex imaging systems Additionally cells may be grown directly in Cytation3 to reduce environmental variation due to manual intervention Now researchers can culture cells and subsequently glean almost simultaneous quantitative and qualitative data Cytation3 s combination of technologies also helps to streamline cell biology research for im proved lab efficiency and increased throughput Biolek Get a Better Reaction www biotek com Lab Manager May 2014 Cytation3 is modular so labs can select only the modes that they need and can upgrade at any time as their needs evolve Microplates trom 6 to 384 wells and microscope slides may be used for a variety of throughput needs Optional dual reagent dispensers may be used for injectand read assays and the optional BioStack3 Microplate Stacker has a plate transfer time of about 8 seconds per microplate for increased throughput and walk away automation of up to 50 microplates CELL BASED ASSAYS Patented Hybrid Technology incorporated in Cytation3 or available as an upgradeable op tion combines filter and monochromator based fluorescence optics in one compact unit for pow er and flexibility in assay choice The filter optics use direct fiberfree light path
41. the scientific community For more information visit www perkinelmer com Multipurpose Imaging System Amersham Imager 600 Series e Designed to enable capture and analysis of high resolution digital images of protein and DNA samples in gels and membranes e Delivers high performance imaging to chemiluminescence fluorescence and colorimetric applications e Wide dynamic range allows weak and strong signals to be quantitated accurately at the same time e Full system automation means there is no need for adjustments after startup GE Healthcare www gelifesciences com TECHNOLOGY NEWS Image Analysis Systems G BOX Chemi XX6 and XX9 Suitable for scientists needing a versatile system capable of exceptional imaging of 1D and 2D gels as well as chemi and fluorescent blots Feature high resolution high quantum efficiency 73 quantum efficiency at 425nm low noise CCD cameras with the highest level 10 95 auto focus lens and 6 and 9 megapixel resolution respectively Provide imaging areas of 32 3cm x 25 6cm Syngene www syngene com SUPPLIES amp CONSUMABLES Single use Waste Collection Bottles VACUSIP Designed for the VACUSIP benchtop aspiration system Allow VACUSIP users to go one step further in terms of operational safety and reducing the cost of handling disposal of biohazardous substances Shatterproof and vacuum resistant bottles eliminate risks of breakage and contamination by the biological agents during
42. them clear direction and then get out of the way Doers like to get things done They want to be given a task and then allowed to do it The best doers often are the go to people every one else relies on to solve the most difficult issues Unity Lab Services Part of Thermo Fisher Scientific There are some times when you need a strong leader who 1s also a doer Perfect Put a capital L and a lower case d in the box Do this for the whole organization When you are done and only then step back and look at the people you have Evaluate each as to their leadership management and doer capabilities Now you can start to put names in the boxes If you are like most you will run out of ap propriate names before you run out of places to use them Consider training coaching and other support to keep good people BUT do not strangle the operation Lab operations need to understand the difference between leaders managers and doers Doers love strong managers who can give them a clearly defined task target and resources then clears the way for them to be successful Good managers want to keep their best doers to the point they will pay them above their standard grade to keep them happy and the company productive The most successful organiza tions start with a clean sheet of paper and design the best possible organizational structure No names just an understanding of where you need l
43. them whether there is a bidding process for their testing that you could enter Also ask whether there is a time when you could give the staff a short presentation or brochures about your lab services Use this time to educate the staff on the importance of environment and water quality to patient health Explain how your lab can help their patients ensure that they do not become sick from materials in their homes radon lead paint etc or water Ask if there is a place where you may leave brochures for patients to pick up Contact doctors or clinics that specialize in pregnant women Find out if they have Lamaze or parenting classes at which you can make a presentation or pass out information about testing options for homes that have infants or small children Take the time to educate the parents about why they should test their water air and paint Find out whether there is a professional group for local realtors or well drillers to which you could talk about your lab services Ask if they give out packets or brochures to homeowners and if they d be willing to include something from your lab Offer to speak at a home buying seminar or set up displays at a home improvement shows or county community fairs Educate current homeowners on the importance of water quality and envi ronmental health in their homes to encourage testing Consider doing quick checks using screening kits for nitrate or lead to show how clear water can actually
44. tion The ability to go completely paperless is no longer bound by the physical constraints of the laboratory itself The LIMS ensures that location information barcode reading precise timing and any other data the technician collects are linked directly to the test results In other words combining mobile devices and a LIMS brings a sampling program s entire chain of custody under one secure umbrella This enables improved regulatory compli ance traceability and auditing of course but 1t also makes for better management iPhone is a registered trademark of Apple Inc One of the greatest advantages of mobile devices is that they re a two way street Not only can users submit data to the LIMS they can examine and look at data on a device without physically entering the lab Today s state of the art LIMS such as Thermo Scientific SampleManager offer connectivity with mobile devices that allow lab personnel to visualize results from high level trends down to granular details For labs performing extensive chromatography runs for example data must constantly be monitored to ensure results match up with reference data Scientists aren t interested in general results alone they want the ability to drill down to the level of individual components and peaks A LIMS provides access to interactive data not just a static image such as a JPEG or PDF from a mobile device allowing the lab to run far more efficiently The same g
45. to analyze smaller sample sizes and features reduced solvent consumption Waters www waters com BASIC LAB Balance Series for Precision Weighing Nimbus e Includes 15 models that deliver a full breadth of precision readabilities ranging from 0 1mg to 0 1g e Innovative design enables a compact footprint so it occupies minimal space but retains a high level of performance aga e Single piece construction offers greater stability enabling highly repeatable results e Features 10 weighing units including a customizable unit to handle a variety of everyday lab work Adam Equipment www adamequipment com www labmanager com TECHNOLOGY NEWS Overhead Stirrer Vortex Blend Caters to scientists who need to test new formulations and blends in parallel Offers scientists an efficient space saving way of performing 3 blending experiments in parallel Benefiting from a 3 way gearbox and integral heating plate the unit economizes valuable lab space and ensures that parallel experiments have identical stirring rate and heating rate temperature conditions thereby improving result reliability Asynt www asynt com Centrifuge Rotor JA 14 50 e Designed for applications that use common disposable conical tubes e Can be used in Avanti J 26S J 301 and J E centrifuges e Capable of spinning 50 15 5 and 1 5 mL conical tubes and 50 15 and 10 mL round bottom tubes and bottles at their maximum speeds e Deliver a
46. to coexist and be talked about Brett explains Then the ideas can be packaged Traditional collaboration Dealing with multicultural teams is hardly a new experience for R amp D managers Most organize collabora tions in one of two ways In the dominant or subgroup www labmanager com ICROBIOLOGICAL 2 INCUBATOI The IE line of NuAire In VitroCell ES Ene rgy saver CU MAI 7 RUN DOOR STATUS Incubators provide a reliable controlled in vitro environment MS for optimum tissue cell culture growth through precise control ie Si ok oe pa dE fi A of humidity temperature gas and contamination control TEMI D po 7 y p Ul The NuTouch Intelligent Interface is a user friendly color touch screen designed for high user adoption Control chamber parameters with the touch of a finger e Maintenance reminders e Historical graphs for gas and temperature e On screen icon descriptions e On screen instructions for calibration and sterilization cycle procedures Learn more online MSN international Federation of www nuaire com 04816 es Blosatety Associations NuAire is a proud corporate sponsor of the IFBA F o E J nuaire nuaire nuaire am nuaire 2100 Fernbrook Lane Plymouth MN 55447 U S A 763 5531270 WWW NUAIRE COM Copyright NuAire l y Lab Answer 28 LEADERSHIP 8 STAFFING coalition model a specific set of team members which may or ma
47. top speed of 14 000 rpm and ref of 35 000 x g Beckman Coulter www beckmancoulter com High Performance Centrifuge Avanti JXN 26 e Offer laboratories an intuitive interface and advanced data management features A Can be run from an Apple iOS or Android device using MobileFuge the only mobile application available to run and manage laboratory centrifuges Provides application versatility with wide rotor compatibility and capacities to 6 L speeds to 26 000 rpm and rcf up to 81 700 xg Beckman Coulter www beckmancoulter com Precision Measurement Instruments Digi Sense o e Line includes an infrared thermometer thermocouple thermometer humidity meter and light meter i A e Additional items soon to be available within the line include a pressure and flow meter thermohygrometer and a series of anemometers e Deliver accurate readings while withstanding rigorous use Cole Parmer www coleparmer com Acrylic Flow Meters 6A03 e Offered with interchangeable direct reading scales for air water argon oxygen carbon dioxide nitrogen helium and hydrogen e Dual scales display flow rates in both metric and English units e Feature a maximum temperature of 130 54 and a maximum pressure of 100 psi 6 89 Bar e Yellow colored back plate enhances the readability of scales and minimizes eye fatigue J LA Dakota Instruments www dakotainstruments com Viscometer Rheometer on a Chip m VROCi Provides me
48. utility for which handhelds excel such as sample tracking and other uses that make sense for tablets Many LIMS vendors now provide some level of service in the cloud known as software as a service SaaS The idea makes sense for many industries particularly those that are highly science based such as GenoLogics next gen sequencing customer base Many start up diagnostics companies have no interest in managing their IT in house Hoover tells Lab Manager They want a LIMS they need one but they don t want the overhead Luckily for them their data is much more secure at a data center than on site and the service is less expensive than an in house installation Downtime 1s also significantly reduced as the LIMS company does not need to travel to the customer for troubleshooting GenoLogics services its cloud customers through Amazon Web Services whose commercial tagline Launch virtual machines and apps 1n minutes illustrates another significant benefit for cloud based LIMS customers virtually no start up time Deploying a LIMS used to be like starting a major construction project Hoover says These benefits have contributed not only to lower prices for functionality equivalent to the most sophisticated systems of a few years ago but also to a democratization of LIMS One repository That is not to say that high end LIMS installed at the customer s brick and mortar facility
49. which cover just the nose and mouth full face respira tors which cover the entire face and hood or helmet style respirators which cover the entire head Lab Manager May 2014 APRs should be used only for materials that have good sensory warning properties such as odor or irritation Respirators can protect the user in two basic ways The first is by cleaning the dirty outside air that passes through a filter or adsorption bed or both when one inhales This type is known as an air purifying respira tor APR The other main type of respirator protects the user by supplying clean breathing air from a remote source The clean air can either be delivered via a supply line supplied air respirators SARs or the clean air is packaged and carried with you in a tank a self contained breathing apparatus SCBA such as by a scuba diver When using APRs one must first have a good idea of the nature and concentration of the contaminants against which one needs protection For example are you seeking protection against actual chemi cal hazards or simply avoiding exposure to unpleasant odors In addition one should ensure that there 1s adequate oxygen at least 19 5 percent by volume in the air because the APR simply cleans the air and will not provide extra oxygen If there is inadequate oxygen as one might find in confined spaces APRs cannot be used and one must use a supplied air ty
50. years will you save enough money in energy costs to pay for your freezer fridge 4 How much sample capacity are you getting for your space 5 What are the optimal voltage wiring conditions for running the fridge freezer If the building is older will low voltage or voltage fluctuations affect the performance of the freezer fridge TOP 10 FEATURES FACTORS respondents look for when purchasing cold storage equipment RELIABILITY OF PRODUCT 79 Ps 00 lt lt DURABILITY OF PRODUCT 70 PoE PRICE 66 O 0000000000000 lt 0 lt 0 lt 0 lt lt SENSITIVITY AND ACCURACY OF TEMPERATURE CONTROLS 55 CJ PARA WARRANTIES 48 O ENERGY EFFICIENT 43 CA ae REPUTATION OF VENDOR 39 E aa R SERVICE AND SUPPORT 39 S A AUDIBLE AND VISUAL HI LO ALARM FOR EACH CONTROLLER 36 For more information on cold storage equipment including useful articles and a list of manufacturers visit www labmanager com fridges freezers www labmanager com ARE YOU IN THE MARKET FOR AN ELECTRONIC LAB NOTEBOOK ELN Electronic laboratory notebooks ELNs one component of a lab s information infrastructure help laboratories capture and manage knowledge streamline data management protect intellectual property and foster collaboration Both non specific generic ELNs which compete directly against paper notebooks amp application task specitic ELNs exist each with their own fans TOP 6 QUESTIONS You Should Ask When Buying an Electro
51. ENTRAL DATA REPOSITORIES REINVENT THEMSELVES by Angelo DePalma PhD espite having been available for close to 30 years laboratory information management systems LIMS are about to undergo a metamorphosis characterized by greater utility accessibility and availability at lower cost The first LIMS implementations resided on mainframe computers at large companies a situation that permeated the product platform for several decades even after personal computers became ubiquitous LIMS technology has lagged behind other software at several levels says Aubree Hoover senior product manager at GenoLogics Life Sciences Software Victoria BC Canada GenoLogics specializes in LIMS for proteomics and genomics particularly next generation gene sequencing Overcoming deployment difficulties LIMS s reputation for difficulty of use high cost a steep learning curve and spotty accessibility 1s about to change as GenoLogics and other firms are offering web based LIMS and expanding accessibility to portable devices such as smartphones and tablets GenoLogics released a cloud based product in 2013 and now supports tablets as well Which raises the question of accessibility versus feature set To some degree the software must be simplified for use on mobile devices Hoover admits Applications requiring significant keyboard input for example are inappropriate for tablets GenoLogics has instead focused ona level of
52. ERING YOUR SUCCESS Balston solutions parker com conservehelium PRODUCT FOCUS LABORATORY GAS GENERATORS Hydrogen generators to the rescue Reliability is a theme in hydrogen generation as well although these systems work on the principle of water electrolysis and do not require gas compression The ongoing helium shortage has created a robust market for hydrogen in gas chromatography GC has a heater and a small enclosed space and the gas is not burning off as it does in FID People think Boom Hydrogen was always part of GC instruments that used flame ionization detection FID Today with helium in short supply companies are turning to hydrogen as a carrier gas which has caused a boom in gas generator sales The shortage remains a hot topic says Kim Myers global product manager at Parker Hannifin Haverhill MA Many companies today are rationing helium or have no access to it whatsoever Those who can get it are paying five times as much as a few years ago Thanks to helium s scarcity vendors of gas generation equipment are enjoying unexpected assistance from instrument companies that now write articles and make presentations on the benefits of hydrogen carrier gas Myers explains Think about it GC has a heater and a small enclosed space and the gas is not burning off as it does in FID People think Boom But because they want to keep selling instruments those companies are
53. OF GCV 46 s high resolution MS data e Allow JEOL data to be imported directly into GC Image e Exact masses and isotopic data provide elemental compositions for molecular ions as well as fragment ions e High resolution selected ion chromatograms make it possible to find target compounds and families of chemical compounds in very complex mixtures JEOL www jeolusa com Data Analysis Software CytoSure Interpret version 4 5 3 e Provides a powerful and easy to use package for the analysis of aCGH data the gold standard for copy number variation CNV detection e Offers many advanced features including Automatic Aberration Classification e Allows the choice of standardized or customized user defined data analysis e Enables users to configure the software to automatically perform an initial classification of any detected CNV Oxford Gene Technology www ogt com www labmanager com PRODUCT SPOTLIGHT CLOUD BASED COLLABORATION ACADEMIC RESEARCH PLATFORM PROVIDES APP BASED APPROACH TO ELECTRONIC DATA CAPTURE AND SHARING BOOTH 112 Following the 247th American Chemical Society National Meeting amp Exposition students and researchers now have a A new tool for scientific collaborations cg PerkinElmer released Elements its cloud based expandable platform Mar 17 Es Elements is a first of its kind tool for academic scientists that changes how they collect and share data and information in the lab and cl
54. ON DEMAND GET BETTER RESULTS WITH A COST EFFICIENT RELIABLE AND SAFE ON SITE GAS GENERATOR FROM PROTON ONSITE Proton Onsite s gas generators meet the needs of any lab big or small Whether you need hydrogen nitrogen or zero air our space saving on site generators offer a safer more dependable and efficient supply of gas for all your GC and LE MS5 needs ProtonOnSite com 203 949 8697 CustomerService ProtonOnsite com PRODUCT FOCUS MICROPLATE READERS MICROPLATE READERS Lab Manager CELL BASED ASSAYS REQUIRE COZY NEW FEATURES by Mike May PhD ife scientists started using microplates in the 1950s with some of the first ones literally machined from solid blocks of plastic You don t need to wander around a molecular biology lab long today to see the progress in microplates some now including thousands of wells Beyond the number of wells biologists also use many plates To analyze the contents scientists use microplate readers and they are advancing just as rapidly as the plates probably even more so The most sophisticated microplate readers help scientists take care of their samples while they analyze them In addition advanced devices provide more analytical capabilities Staying alive When asked about the most interesting new features in microplate readers Xavier Amouretti manager product marketing at Bio Tek Instruments Winooski VT says Cell based assays drive most of them He
55. PERSPECTIVE ON Insights IMAGING SYSTEMS UYERS REPORT WWW LABMANAGER COM Lab Mana Run Your Lab Like a Business T i e INFORMATICS MANAGEMENT SYSTEM AFOOD AND BEVERAGE LAB LAB TECHNOLOG ger May 2014 Volume 9 Number 4 i GREATER TEST MOBILE OFFERINGS DELIVER EN SECURITY AND COMPLIANCE THE HUMAN SIDE MANAGING CULTURALLY DIVERSE TEAMS MVE CELL EXPERIMENT Tecan s washers and plate readers offer the perfect combination IN PROGRESS to yield a high cell number during assay preparation and to 18 maintain a perfect environment for long term experiments Ingenious optical features and remote monitoring guarantee 1 N high quality of your assay results ba Infinite 200 PRO OR You can maximize cell numbers during assay preparation with i fully controlled cell washing procedures F ic Protect cell viability for live kinetic assays with the integrated X 8 gas control module GCM 6 Benefit from consistent results by detecting every cell using 4 the high sensitivity cell reading mode of the Infinite family of 2 microplate readers o Monitor your experiments remotely using Tecan s Common aii JR cean Notification System CNS on your mobile device Cell number To see how we are shaping the lab of the future Improved well to well uniformity of 50 results with an up to 25 increase in signal sensitivity of GFP transfected A431 carcinoma cells register for our webinar series using enhanced
56. RE THE FIRST OF ITS KIND LEAK PROOF ECONOMICAL POLY BOTTLE ESS Environmental Sampling Supply the leading source for PreCleaned Certified and PrePreserved sample containers introduces CapSure a revolutionary line of light weight leak proof HDPE containers Designed as a mated pair container and closure the closure incorporates a full three turn thread and a plug seal ring For single use Environmental Analytical or packaging applications In its effort to design the perfect bottle ESS developed CapSure in the popular Oblong style with a wide mouth opening It incorporates graduation in mL and ounces ESS offers leak proof performance with single use savings Contact ESS Environmental Sampling Supply 800 233 8425 www essvial com Still the Best Selection of Research Lamps Anywhere www sonntek com sonntek aol com Lab Manager May 2014 RAISING RESEARCH BY INTELLIGENT DESIGN NuAire s In VitroCell ES Energy Saver Microbiological C02 Incubators features seven direct heat models with varying Humidity Oxygen and Fuel Cell controls Designed with the NuTouch Intelligent Interface a user friendly color touch screen offered in English Spanish German and French Chamber parameters are easily controlled recorded chamber performance can be viewed onscreen or downloaded via USB In VitroCell ES incubators provide constant contamination control C3 against potential contaminates that ma
57. S VISIBLE by Angelo DePalma PhD maging encompasses a wide range of techniques that enable visualization of hidden features of samples structures or organisms Imaging occurs at many scales from medical magnetic resonance imaging of patients to individual atoms This INSIGHTS on Imaging Systems focuses on the lower end of size domains in the typical operating range of but not limited to microscopy Microscope based imaging used to be the domain of core facilities and dedicated operator directors Today imaging has become routine not quite to the technician level for all techniques but moving in that direction Motorized stages and other automation tools are enabling unattended imaging of multiple samples Confocal systems that once required a dark room now sit on fully lighted laboratory benches But as microscopy advances and the heart cut methods become more democra tized and accessible the leading edge becomes more complex in terms of science and instrumentation Together these advances change how people address ques tions in biology says Brendan Brinkman senior product manager for laser scan ning confocal microscopes at Olympus Center Valley PA The same individuals who might have used a benchtop fluorescence microscope several years ago now have access to imaging systems that were the exclusive tools of research labs Microscopic imaging has come a long way during the past decade Brinkman adds
58. US AT WWW NAOSMM ORG JOIN NOW TO TAKE ADVANTAGE OF e Networking with other members in the same field e Exclusive promotions and savings from supporting Corporate Members e Advance your career with education and cetrification programs e Interaction with scientific supply companies at annual trade show e Recognition of member volunteers e Quarterly magazine Newsline with insightful articles e NAOSMM Listserv and Forum enables members to interact 24 7 e Online membership directory makes contacting members easy CORPORATE MEMBERSHIP AND SPONSORSHIP OPPORTUNITIES ARE AVAILABLE TO BECOME A MEMBER GO TO WWW NAOSMM ORG 70 TECHNOLOGYN EWS Multi Detector Bio SEC Solution NS 1260 Infinity FA I i l BOOTH 84 e e Features advanced light scattering detection capabilities fully bio inert instrumentation gt MD high resolution columns and intuitive software L E 0 e Provides biopharmaceutical researchers with a ee previously unattainable robust measurement capabilities with excellent reproducibility Also provides maximum sensitivity and accuracy through advanced detection capabilities Can significantly simplify and speed up workflow reducing the valuable time and expense associated with bringing biopharmaceuticals to market Agilent www agilent com High Resolution Array ICP OES PlasmaQuant PO 9000 e Developed to master the most complicated analytical challenges in emission spectrometr
59. a general fluorescent signal of a well will be very beneficial to our research More and more people in research labs are working with live cells in microplates Dialing in sensitivity Despite the advances available in microplate readers not every user runs complicated experiments Many of the assays that have been run in microplate readers are still used like simple concentration measurements or looking at characteristics of a specific molecule Amouretti explains Some users though need more options As an example Ronald L Earp president of BMG LABTECH Cary NC describes using a linear variable filter LVF monochromator It offers essentially an infinitely adjustable filter he says You can set the center wavelength and set the bandpass Traditional monochromators have only one or few specific bandpasses In Earp s instrument he says You can increase or decrease the bandpass of the LVF monochromator with a click and drag of a mouse This capability proves incredibly valuable when working with fluorescent proteins with various emission characteristics There are multitudes of them Earp says You d need more than thirty filters to measure them all An LVF monochromator can just be adjusted for different fluorescent or luminescent labels It s also possible to incorporate an LVF dichroic that enhances a microplate reader s sensitivity This combination says Ea
60. access 4 Workflow coordination across geographic 4 and business boundaries Other 2 5 For more information on electronic lab notebooks including useful articles and a list of manufacturers visit www labmanager com ELN May 2014 Lab Manager 77 SURVEY SAYS Types of pipettes used by survey respondents Manual Single channel Manual Multi channel Manual Fixed volume Electronic Single channel Electronic Multi channel Electronic Fixed volume Repeater Other Estimated daily time spent using a pipette as reported by survey respondents Less than 1 hour 1 2 hours 2 3 hours over 3 hours Nearly 40 of respondents plan on purchasing a pipette in the next year The reasons for these purchases are as follows E Replacement of aging pipette E Addition to existing systems increase capacity E Setting up a new lab 32 19 14 10 8 3 13 1 25 31 23 22 E Moving from a handheld pipette to an automatic pipette E Other Need a more ergonomic pipette 1 ARE YOU IN THE MARKET FOR A PIPETTE Pipettes can be found in almost every laboratory and if you re looking to buy one there are many options manual or electronic single or multi channel Luckily a few main considerations can help in deciding whether or not to buy the latest pipette technology TOP 5 QUESTIONS You Should Ask When Buying a Pipette U A 0 N What differentiates the pipette from othe
61. are going away any time soon Mike Kelly sales director at LabWare Wilmington DE notes that over the years many organizations have added layer upon layer of LIMS Kelly notes a large pharmaceutical customer he visited a potential customer already had seven LIMS installed The situation which arose due to the siloization of data segments at large companies is not unique Kelly says But it flies in the face of what a LIMS is supposed to do which 1s to provide a single repository for all data Today s life sciences organizations however are beginning to recognize the benefits of collaboration across research development diagnostics and patient care Creating a single log in integrating as much data as possible with the same accuracy checks on all data facilitates collaboration Kelly adds You can t merge seven tasks into one when the LIMS can t see five or six of those tasks The importance of one LIMS one log in one repository to highly integrated teams is exemplified by hospitals that conduct translational medicine These organizations house laboratories that conduct basic research patient records diagnostics labs and clinicians Making sense of projects that flow from the benchtop to the bedside would be impossible with seven LIMS but enhanced by one such overarching all encompassing product Angelo DePalma is a freelance writer living in Newton NF You can reach him at an
62. ares STEM to a slide projector that illuminates a larger area to collect data and transmit 1t to a CCD camera TEM in particular has evolved as a cutting edge tech nique for atomic imaging but it was limited to visualiz ing atoms 1n black and white It is now possible through advanced imaging to assign colors to atoms based on spectroscopic properties This allows us to visualize chemical information Freitag says The two techniques employed by FEI are energy dispersive X ray analysis EDS and electron energy loss spectroscopy EELS Another advanced STEM technique differential phase contrast allows measurement of electric fields between atoms which are directly linked to the physical properties we experience in the macroscopic world The technique also visualizes electric fields between atoms which are significant in polar ceramics such as gallium nitride used to manufacture light emitting diodes These fields are directly linked to light emitting properties so understand ing them can help in the design or improvement of these materials Freitag says Information at the atomic scale provides many significant insights into the macroscopic properties of materials The observation of variation of atomic distances can provide information about strain for example in silicon lattices of nanodevices which directly relates to electron mobility and faster switching transistors A Ultrafast Electr
63. aring that can make them more efficient in the lab Even scientists who are already using an electronic laboratory notebook ELN still have to walk out of the lab to enter data into their ELN To remember the data they write them down on sticky notes paper sheets or forms even on the palms of their hands This process is disruptive time consuming error prone and adds no value Scientists also typically print out workflow procedures on paper and then have to bring this documentation into the lab which is not efficient Although some laboratories have kiosk PCs for data entry in the laboratory scientists still have to walk to these PCs as they are anchored They also need to share them and might have to switch between different applications running on these PCs which adds non value added steps to the process Large tablet PCs loaded with full applications are an option for going mobile in the lab However they too have not been successful as they do not allow for quick data entry fat fingers syndrome and they are not as easy to carry as small mobile devices Also the hardware requirements for some labs ruggedness resistance to chemicals etc might not be fulfilled Solution The most effective and efficient solution to this lab informatics challenge 1s to capture data on smaller mobile devices that can easily be carried around like in a h lab coat pocket This requires a dedicated application with a clean and in
64. assroom The platform provides a cost efficient solution for safe secure record keeping that eliminates the need for investment in digital infrastructure Despite the wide range of technology offerings available in the marketplace today a recent study we conducted revealed that more than 75 percent of research academics still use a paper notebook for recording lab notes said Michael Elliott CEO and chief analyst at Atrium Research amp Consulting Electronic lab notebooks are changing this practice in how labs at major universities embrace electronic data capture cloud based applications and collaborative tools to modernize their research environments Elements expandable platform delivers an application framework for deep scientific support through modular apps that can be combined into sophisticated scientific experiments Being 100 percent cloud based also allows users to be up and running in minutes without the need for lengthy deployment and installation steps The new tool features electronic data capture and ChemDraw integration as well The Elements tool matches workflows with the needs of each type of science and scientist said Daniel R Marshak chief scientific officer at PerkinElmer This technology empowers academics to work more effectively toward advancing their research in human and environmental health We will continue to launch additional platform enhancements in response to the insights we receive from
65. asurement for low viscosity industrial fluids under conditions that reflect those that apply during end product use Features completely enclosed measurement for safe and reliable analysis of highly volatile or environmentally sensitive samples Generates accurate viscosity data using sample volumes as low as 50 uL Also boasts a wide dynamic range easy sample loading and repeatable accurate measurement www malvern com Malvern High Capacity Gas Purifier Model 8000A Features a rated operating pressure of 1500 psig 14 NPT female side inlet and top outlet are machined directly into the purifier body eliminating the more fragile connections of previously available units Uses new improved cartridges that can also be used with gas purifier housings already in service supplied by SGD Matheson and all other companies offering similar purifiers El SGD www sgd com Tube Cooler Module 7 Asia par e Allows fluoropolymer and stainless steel Asia tube reactors to be used at ambient temperature or cooled to sub ambient CELD temperatures as low as 68 C depending on the cooling khos A medium of choice e Can be operated for up to four hours before refilling the cooling medium e Unit can also be used as a standalone module or connected to an Asia heater Syrris http syrris com May 2014 Lab Manager 73 74 TECHNOLOGY NEWS Wireless Handheld Thermometer RTR 601 e Designed to measure the internal tem
66. aving to send samples out or bring in an MS expert This is only possible if the transition to MS from traditional detectors is seamless MS had to be as familiar and accessible to analytical scientists as their optical detectors are When designing the ACQUITY QDa Detector Waters attempted to duplicate users familiarity with optical detectors with respect to use size affordability and software integration The knocks against MS have been high cost and its requirement of specialized expertise From an end user s perspective achieving ease of use 1s perhaps the most significant hurdle to adopting MS which is still for many an intimidating technology Unlike conventional MS instrumentation which requires optimization and tuning for different separations the ACQUITY QDa Detector is pre optimized and runs without adjustments or tuning for most samples Users have very little to do except turn iton The ACQUITY QDa Detector 1s as close as you can get to out of the box MS Read tells Lab Manager Competitive threats MS detection adds anywhere from 100 000 to 500 000 to the cost of an LC or GC system according to Jason Weisenseel PhD technical leader at PerkinElmer Orlando FL That puts it outside the scope of many laboratories that expect to pay a great deal less for the separations platform itself The immediate benefits depending on the type of MS are additional mass and fragment informatio
67. ay for Rigaku Raman said company general manager Bree Allen at Rigaku s Pittcon press conference on Mar 4 Progeny will reset customer expectations in the market because when compared to other handhelds available today if offers a level of functionality and performance that simply has not been achievable in the past The instrument has an advanced miniature VPG based 1064nm optical engine at its core offering benchtop quality analytical performance in a rugged ergonomic and IP 68 sealed enclosure Progeny s fully customizable workflow software is 21 CFR Part 11 compliant and boasts a smartphone inspired user interface which shortens the learning curve allows rapid implementation of material ID methods and improves return on investment The handheld also features a unique angled wand shape which is designed to be used with one hand unlike the heavy bulky gun shaped designs of the past The shape of the Progeny facilitates entry into closed spaces Allen explained adding that this makes it much easier for incoming material inspection in the pharmaceutical world and for safety and security markets The handheld also uses a patent pending high selectivity algorithm for material ID and has the ability to develop and run advanced quantitative methods directly on board These features ensure accuracy Allen said When it comes down to it it improves confidence in testing results he said For more information
68. ays write everything down and keep lab notebooks simple limiting the infor mation recorded to the purpose equipment materials pa rameters raw data and observations rather than including complicated calculations for resulting data analysis www labmanager com TECHNOLOGY These extra calculations add more for the reviewer to check and it wastes time she explains The raw data can later be entered into a pre established spreadsheet such as Excel which performs the calculations and graphing for you This way you can be relatively sure of the accuracy of the data and spend time focusing on the interpretation not crunching and reviewing data Schwartz says having a plan in place for documenta tion right from the start is essential That is a much easier way to do things than to decide a year or two down the line that you really should be documenting things better because then you have to go and dig through all these old records to bring them up to speed he says The single best piece of advice I would give is to make it a priority of the lab such that it s done right from the beginning Not doing documentation properly can lead to much big ger problems than having to retrieve and update old files It would be detrimental to our R amp D and potentially harmful to the development of a new product Ma tosevic says about what would happen if Akron didn t ELGA Our innovation Your choic
69. ble to replicate the experiment and problems were found with the presentation and handling of the stem cell images data and text in the papers leading some to claim they were altered or faked From this incident it s clear that proper documenta tion is essential to ensure that the data from cell culture research is accurate and to prevent incorrect information from being published If you don t accurately document what you ve done in your experiment then you can t accurately publish that material or if you do then it could have some flaws in it says Philip H Schwartz PhD director of the National Human Neural Stem Cell Resource at the Children s Hospital of Orange County Research Institute Orange CA Documentation and writing in your lab notebook etc is something that we train our scientists to do even when they re in middle school Documentation is also necessary to allow cell culture labs to solve any problems they may encounter in experi ments as it lets them trace such problems back to their Lab Manager May 2014 WHY KEEPING TRACK OF EVERYTHING IS ESSENTIAL IN THE CELL CULTURE WORLD SSS A source That means that everything these labs use in their experiments such as reagents and even plasticware must be tracked as there are differences in how those supplies and equipment are manufactured that could impact cell culture capabilities Schwartz explains If we did not keep accurat
70. bmanager com celllineexpert 64 E INSIGHTS ON IMAGING SYSTEMS A Microscopy 1s an ideal imaging platform because 1t op erates in size domains that are generally invisible to the naked eye The application of spectroscopy e g Raman infrared ultraviolet fluorescence to microscopy further expands microscopic visualization of chemical compo nents that appear identical with visible light microscopy Chemical mapping of materials tissues and cells has become the leading edge of microscale imaging To illustrate the importance of microscope based imaging across size domains of many orders of magnitude consider that a fair amount of diagnostic imaging today occurs not on arms legs and livers or on tissues or cells but at the molecular level for example fluorescence in situ hybrid ization FISH which is described in greater detail below IMAGING BY MASS SPECTROMETRY Microscopy 1s not the only imaging platform suit able for microscale samples and events The ability to discriminate on the basis of molecular weight is what confers similarly useful imaging capabilities on mass spectrometry Although MS is not microscopy tech niques that combine sampling of very small regions on samples gentle ionization and software that renders mass data and physical coordinates into composition maps have pushed MS to the forefront of advanced imaging technologies albeit one that involves greater capital expenses than light microscopy
71. boratory practices Gene Tetreault senior technical director of analyti cal development quality and manufacturing at Accelrys which has developed mobile data recording apps for lab informatics says The intention is to be running on a mobile device while walking around the lab while conduct ing procedures Accelrys Capture the first mobile app the company has created for the Windows 8 1 mobile operating system was designed to enable scientists to move around their labs and record information in conjunction with their electronic laboratory notebook ELN experiments Tet reault says this app facilitates the capture of observations pictures and annotations among other information Accelrys refers to Capture as next generation mobile We are taking advantage of the fact that finally the hard ware has caught up to us and everyone now has a mobile device a tablet or an iPhone or an Android device and it is very accepted now to have an app that does specific functions says Tetreault This next generation of apps will do a lot of the same functions we do today manag ing procedures capturing data integrating with equip ment performing calculations but in the next genera tion format Lab Manager May 2014 Tetreault says that laboratory informatics focus on plan ning managing executing and reviewing relevant work processes which follow the same flow as overall laboratory operations He says
72. by spectroscopy whereas historically systems have worked the other way around A TWIST ON FLUORESCENCE Through its use of fluorescently labeled tags fluores cence microscopy provides a means of targeting specific structures inside cells May 2014 Lab Manager 65 66 INSIGHTS ON IMAGING SYSTEMS Fluorescence in situ hybridization FISH is a tech nique that images abnormal genes that are indicative of disease In cancer disease management for example FISH detects targeted DNA abnormalities and 1s widely used for diagnosis prognosis and treatment selection Microscope manufacturers are particularly interested in FISH because it uses fluorescence to image very small objects In 2013 Nikon Instruments entered an agree ment with Cancer Genetics Rutherford NJ to distrib ute FISH DNA probes for oncology The deal brings together the two ingredients necessary for DNA level diagnostic imaging fluorescent FISH probes from Cancer Genetics and a suitable microscopy imaging platform from Nikon In this instance the genetic tests will take advantage of a technique Nikon has developed for performing simultaneous multicolor FISH testing and complex image analysis rl z 4 3 p rm 2 A Multipoint Scanning Confocal Microscope Opterra Bruker www bruker com While the technology behind FISH is not new fluores cence based techniques are rapidly evolving together with and because of instrumentatio
73. ccess to past analyses and knowledge proved invaluable but elusive It became clear that the company was generating far more data than any one individual could keep in their head Steve led the efforts to find a suitable platform to store search and share their data globally Such a database mitigated the risk of duplicated effort but required a deep dive into the dark arts of informatics His success was measured by the quality of the resulting repository of knowledge that didnt forget go senile retire or leave the company for a competitor as painful as pulling teeth then you end up with compliance issues Getting away from the power of memory was the second criterion and with moving to a database that was a given We also needed the internal processes and backups in place to make sure that if the data became corrupted we could wind back a couple of days and retrieve the stored data Other criteria for selection included ways to speed up the process of interpretation by being able to look at and interrogate the data be longing to a colleague who is located across the Atlantic This would be akin to looking into his lab notebook to see what he has worked on in order to help with a similar problem that am facing Other criteria included improving the contidence we had in the hits we got from the database reducing the likelihood of making mistakes in elucidating the data and increasing our ability to share the data when we wanted t
74. cess involved analyzing internal needs evaluating several options and finding the right informatics solution to give GSK scientists access to each others findings to prevent error repetition or inefficiency Q Can you tell us a little about your department and the work that you do A am in the Biotransformation and Drug Dispo sition group at GSK with about 40 scientists at our location here in Ware UK In drug development we are a hub for drug metabolism and pharmacokinetic DMPK studies looking to make sure that a benign drug hasn t been turned into something toxic as the body tries to get rid of it We have a mix of chemists biochemists and biologists and many are analyti cal specialists for the spectral identification of the structure of small molecules So many people with whom work have expertise in mass spectrometry MS or nuclear magnetic resonance NMR or both We work with fairly high end NMR instruments that are powered with cryoprobes that give us exquisite sensitivity to be able to get informa tion trom very small amounts of material that we get back trom clinical trials For MS we have matrix assisted laser absorption desorption MALDI and time of flight TOF instruments as we need the power of these instruments to be able to tease out the materials trom the complex biological matrices that they come buried in Lab Manager May 2014 Q Working with different types of instruments and data what kin
75. ch would need many small generators to meet the full demand of FID and or Carrier Gas requirements and that is often not economically viable Lab managers in large facilities can avoid having to invest in numerous gas generators by installing a single larger Proton OnSite hydrogen generator and plumb ing it into each lab as a lab server That way they can receive a stream of gas at the flick of a switch anywhere in the building A Unique Proposition Proton OnSite s large PEM electrolyzers are the only generators in the laboratory market with the ability to run as a Lab Server One S Series hydrogen generator can supply up to 200 GC units with ultra high purity hydrogen gas that can be maintained and managed from a single source Proton OnSite s technol ogy can also ensure that pressure flow and purity are constant throughout the building and can be monitored at all times Safety First Ensuring safety is paramount for a facility that deals with thousands of liters of hydrogen gas each day considering a single hydrogen cylin der storing 6 300 liters of gas has the explosive potential of 35 Ibs of TNT A facility with hun dreds of GC systems fed by cylinders of hydro gen has a tremendous explosive potential so a lab manager that opts for delivered cylinders has to invest in significant safety infrastructure to mitigate those risks By replacing those cylinders with a single centralized Proton OnSite hydro gen gas g
76. citing the OH bonds in water thereby generating an uncharged ablation plume that ionizes in contact with charged electrospray and sweeps into the MS Angelo DePalma is a freelance writer living in Newton NF You can reach him at angelo adepalma com FOR ADDITIONAL RESOURCES ON MASS SPECTROMETERS INCLUDING USEFUL ARTICLES AND A LIST OF MANUFACTURERS VISIT WWW LABMANAGER COM MASS_SPEC May 2014 Lab Manager 51 PRODUCT FOCUS VISCOMETERS VISCOMETERS THE RANGE OF MEASUREMENT CONDITIONS KEEPS EXPANDING UP AND DOWN by Mike May PhD T he ease of adding ketchup from a squeeze bottle to an order of French fries the trouble that it takes to press toothpaste out of a tube and many other common needs depend on viscosity If toothpaste for example is not viscous enough it would just pour out of control from the tube if it s too viscous you might need a steamroller to get something on your brush In discussing the measurement of viscosity overall Bob McGregor manager of global marketing and high end instrument sales at Brookfield Engineering Middleboro MA says It s a huge area In any industry with materials that physically flow you can be sure they ve been tested for viscosity The options for measuring viscosity are just as numerous as the applications and growing When asked about exciting trends in viscosity measurement Eric Swertfeger director for viscosity products at Anton Paar Graz Austria turn
77. ckner says User error is often the root cause of most problems with operating a centrifuge That is typically imbalance maybe not paying enough attention to the balance of the samples across the axis Many of these issues can be avoided by reading the user s manual or consulting with the manufac turer and taking advantage of any training pro grams they have available Merli says that centrifuges are advancing to a point where very little maintenance will be required The best way to do maintenance is not only instruction from the manufacturer but it would of course be to have a system that doesn t need any maintenance at all Merli says Be sure to check out our upcoming June Mainte nance Matters where we ll bring you great tips on looking after your lab washer A good time to check O rings gaskets and other parts for wear is when installing or removing rotors Photo of the Optima MAX XP ultracentrifuge is courtesy of Beckman Coulter Life Sciences PRECISION ADVANCED MANUFACTURING Introducing the first Cold Frame C F Incubator the most energy efficient incubator available Our new C F Incubators are an all natural energy efficient method ideal for incubating micro organisms and cell cultures without the hassle of complicated monitoring systems or controls START YOUR CULTURES OUTSIDE EARLY THIS YEAR USING THE POWER OF THE SUN Features e Ambient to slightly ab
78. ctionalities could help increase sales of the underlying products as in the case of the Sensorex PH 1 Meter accessory for iPhone and 1Pod and the next generation SAM 1 meter which was launched at Pittcon 2014 Both apps are available as free downloads Launched eighteen months ago the PH 1 is essentially a igh productivity and hospitals nn tection for Your health is our commitment pH meter for a smartphone or a smart device Ben Barker sales manager at Sen sorex Corp says that Apple devices were selected because of their prominence on the market The PH 1 essentially entailed plugging a pH module into an Apple device which converted the analog signal from the module into a digital signal on the smart device and accessing its rich array of features including GPS capabili ties and email Barker says that following considerable success with the PH 1 Sensorex looked for ways to expand 1ts sensors and to use this approach on the Android platform This resulted in the SAM 1 app Instead of plugging in the pH module via the thirty pin jack on the bottom of the Ap ple iPhone the SAM 1 goes through the audio jack of the Android devices Barker says We have also expanded the sen sors We now have the pH electrodes and conductivity sensors and we are looking to add dissolved oxygen sensors and some specific 10n electrodes in the future This is consistent with the broader di rection these days
79. d Avanti centrifuges comfortable to use for peo ple of all heights The handsfree lid swings up and out with the tap of a foot pedal Switching our rotors is quick simple and sate Protected Access Users can be given appropriate access includ ing administrator superuser or operator Limit ing centrituge access only to users that have been properly trained increases overall satety maximizes uptime and minimizes repair costs caused by user error Detailed Run History and Rotor Tracking The system tracks your rotors by serial num bers eliminating the need for rotor log books It also ensures that details of every run are recorded and linked to a specitic user which adds a level of accountability Detailed run tracking builds an archive that includes the user operating the centrifuge date time run parameters and any diagnostics or run errors Users also can track the number of cycles accumu lated on specitic rotors used with the centrituge Then just choose the rotor by serial number from an on screen rotor library The centrifuge tracks the cycles so the life of the rotor can be serviced as necessary and retired before it becomes unsate Beckman Coulter offers the widest range of rotors and accessories designed manufactured and test ed as a part of total centrifuge systems The Avanti JXN is is backward compatible with Avanti J series rotors SO you can maximize your investment Freedom The MobileFuge f
80. d here with permission A SOCOREX SWISS Trust the Experts Today and Tomorrow Mastering precision liquid volumetry for over 50 years socorex has contributed to the success of many scientists generation after generation Choose among a wide selection of highly reliable instruments such as manual and electronic pipettes dispensers and repeaters for all dosing applications Learn how to save 30 on Pipettes with our Starter Kit WHEATON 1501 North 10th Street Millville NJ 08332 856 825 1100 www wheaton com May 2014 Lab Manager 29 30 TECHNOLOGY by Rachel Muenz ocumentation is something all scientists learn to D do when they first start taking science classes in elementary school But keeping track of every thing is especially critical in the cell culture field as illustrated by a recent case in Japan where on April 1 Haruko Obokata a prominent stem cell researcher from the RIKEN Center for Developmental Biology in Kobe was found guilty of misconduct after other scientists questioned her research findings Those findings pub lished in two papers in January were subjected to a com mittee investigation made up of a group of independent researchers from RIKEN Obokata s research involved a technique known as stimulus triggered activation of pluripotency STAP which claimed to allow for easier creation of stem cells using regular cells However other researchers were una
81. d of informational challenges do you face A For us to be able to get a coherent picture of what the body does to our drug molecules we need to be able to bring all the data together into one place just like humans bringing together pieces of a jigsaw puzzle We have two techniques that are complementary to each other We have the sensitivity of MS along with the selectivity of NMR and we need all that data put together to be able to find the molecular structure Prior to 2009 that place was an analyst s head We had a very talented analyst who had been with the company for decades and when he retired we realized just how reliant we were on people s memories The call then went out to get a database approach to try to replace that dependence on human memory Q What did getting this database involve A We had to involve ourselves in a due dili gence process to go out and see what was avail able in the marketplace at that time that fitted our worktlow and the success criteria we were looking to achieve We looked at a number of different vendors and found that ACD ChemFold er Enterprise and ACD SpecManager Enterprise from ACD Labs fitted our worktlow best It was a work in progress since the two pieces ot sottware managed structural schema and spectral data in two separate databases We wanted it to hold integrated structural and spectral data for a com plete biotransformation map the body pro duces upwards of 50 metabolites
82. do don t do it LSI has paraphrased the Bell System slogan in one of ours No lesson is so important and no task so urgent that we cannot take time to teach learn and practice science safely These kinds of attitudes and values are built over time by companies and institutions that make it very clear that they value safety Educators need to have the time as part of their regular working day to set up and test experiments to look up the hazards of chemicals and to tind out what protective equipment and protective facilities are needed This IS the job There s an interesting quote from Jacob Riiss When nothing seems to help go and look at a stone cutter hammering away at his rock perhaps a hundred times without so much as a crack showing in it Yet at the hundred and first blow it will split in two and I Know it was not that blow that did it but all that had gone before Source Kaufman James A Laboratory Safety Guidelines Expanded Edition The Laboratory Safety Institute www labsafetyinstitute org the type of work done the effort expended by the user and the nature and concentration of contami nants encountered The technical assistance group of your respirator manufacturer can help establish these change out schedules A certified industrial hygienist CIH can conduct exposure assessments to determine contaminant levels and establish change out criteria through actual sampling of repres
83. dustrial hygienist certified by the American Board of Industrial Hygiene and the senior industrial hygienist in the University of Florida s Environmental Health and Safety Division He has 25 years of occupa tional health and safety experience at the University of Florida and he spectal izes in conducting exposure assessments and health hazard evaluations for the university s 3 000 plus research laboratories May 2014 Lab Manager 41 Buck Scientific lon Chromatography systems deliver the ease of use and reliability that you have come to expect from Buck Dual overlapping piston pump Microprocessor control for low pulsing Chemically inert ow path Precisely thermostatted cell Low dispersion heat exchanger Dual column oven Leak detection PowerStream data processing PowerStream system control In line vacuum degassing Regenerative chemical suppression IC using UV detector Suppressor cartridge in oven Modular Flexibility The system is easy to use by control panel operation or by using the sophisticated Cecil PowerStream software for data acquisition processing and reporting Full system control is also provided by the workstation equipped with PowerStream The modularity of the system en ables its configuration to be changed with ease for instance to use a UV Visible detector a PowerStream Features Digital and analogue outputs Process signals from multiple detectors Comprehensive integration parameter
84. e document everything correctly Regulations require detailed experimental procedures to be recorded so not having that would be a big issue References 1 Haruko Obokata Who Claimed Stem Cell Break through Found Guilty of Scientific Misconduct Newsweek April 2014 http www newsweek com ha ruko obokata who claimed stem cell breakthrough found guilty scientific 239000 2 Information update on STAP cell research and publica tons RIKEN Center for Developmental Biology April 2014 http www cdb riken jp en index_stap html Rachel Muenz assistant editor for Lab Manager can be reached at rachelm labmanager com or by phone at 888 781 0328 x 233 In tune with your pure water needs Introducing the pioneering PURELAB Chorus range innovation meets choice a fresh new approach to the way you work Want to see more Visit www elgalabwater com purelab chorus 877 315 3542 Email elga usa veoliawater com Part of the world s leading water services company Veolia Water Solutions amp Technologies Q VEOLIA WATER Solutions amp Technologies 36 ASK THE EXPERT Steve Thomas ASK THE EXPERT Steve Thomas an investigator within the Drug Metabolism and Pharmacokinetics department at GSK talks to contributing editor Tanuja Koppal PhD about his experiences implementing a database of metabolic knowledge that helps the company Store share and search data around the globe The pro
85. e documentation of the equipment and reagents and supplies that we use we couldn t backtrack to find problems he says We d be stumbling around in the dark when things went wrong Documenting everything 1s also critical for Sandro Matosevic senior scientist at Akron Biotech Boca Ra ton FL for those same reasons Proper documentation is crucial in maintaining a record of experimental procedures during research and development and particularly when working with a new product or technique Matosevic explains Such tracking is important at Akron a global supplier that manufac tures and distributes components and raw materials for the cell therapy industry for two other reasons Documentation 1s also important for reproducibility and to allow different scientists to share experimen tal details and procedures which helps in maintaining consistency and for training purposes Matosevic says Finally thorough documentation is of critical impor tance for regulated labs such as ours as it is a crucial part of meeting regulations For Simin Zaidi vice president of operations and bio processing at Stamford Bioprocess Technologies Santa Ana CA documentation is critical to the company s cell culture lab which develops cell lines or processes that are used in clinical product manufacturing Cell line traceability and traceability of design data why we have selected a certain process parameter for
86. e experiments are being updated Tetreault notes that with the Capture app data is collected immediately and will not need to be transcribed tremendously improving accuracy Capture works very closely with the Accelrys ELN as an add on capability for a relatively modest additional cost according to Tetreault He foresees the app working as an add on to other vendors ELNs or LIMS in the future Tetreault acknowledges that older and younger people may approach apps differently He says that the pioneer www labmanager com BEYOND LC waters con BEYOND GC BEYOND SFC THERE S CONVERGENCE CHROMATOGRAPHY A new category of separations science Driven by the ultimate selectivity tool Take the unrealized potential of SFC Combine it with Waters legendary UPLC technology and the ability to manipulate gas and liquid phases in one amazing instrument and you ve got ACQUITY UPC That s UltraPerformance Convergence Chromatography And it s compatible with the broadest range of solvents and chemistries This is game changing selectivity with workflow enhancements that will take you further faster than you thought possible See where your lab can go with it Take the UPC Challenge at UPC2 waters com oo E a _ a Z E E 5 O p n m u a ate LS et is a ai Maren Waters T ee ss B ace a THE SCIENCE OF WHAT S POSSIBLE Pharmaceutical Health Sciences Food amp Envi
87. e past 50 years have relied on dyes stains and light microscopy and take many days MS imaging will enable chemical composition data mapping but more important make this type of data accessible to clinicians and researchers Dr Zoltan Takats at Imperial College calls MS imaging a paradigm changer Instead of defining tissue types by their structure we can define them by their chemical composition This method 1s inde pendent of the user it s based on numerical data rather than a specialist s eyes and it can tell you much more in one test than histology can show in many tests RAMAN MICROSCOPY UNVEILING THE INVISIBLE Raman microscopy complements optical microscopy and other imaging tools by providing chemical and morphological analysis at the same microscopic level of detail Because Raman measures molecular bond vibra tions essentially chemical fingerprints users obtain information that 1s inaccessible via light microscopy Raman reveals otherwise invisible information to in crease understanding and solve problems in a wide range of materials from pharmaceutical tablets to graphene monolayers to minerals says Scot Ellis marketing man ager for Raman spectroscopy and microscopy at Thermo A Raman Imaging Microscope DXR xi Thermo Fisher Scientific www thermoscientific com Fisher Scientific Raman is becoming an essential com petitive tool in academic and industrial research Because it r
88. eaders managers and doers with too many of the wrong people If I can paraphrase Jim Collins Good to Great get the right people in the right seats on the right bus It will be much easier to succeed Steve A Epner CSP Steve is the founder of BSW Consulting Group LLC Dur ing his 40 year career he has built a reputation for assisting clients with the integration of strategic business plan ning information systems and tactical requirements Visit www BSWLLC com or call 1 888 279 2792 Be sure to attend Steve Epner s Lab Manager Academy webinar Why Are Some Labs More Productive than Others Understanding to watch the archived video LABCAST May 2014 Lab Manager LMD Is the Answer on Wednesday June 4 or afterward at www labmanager com waters com fy ODIO FOON BRING EVERYTHING IN YOUR LABORATORY TOGETHER yon Aiono AND WE MEAN EVERYTHING O FEATURING LE TECHNOLOGIES Introducing the world s most comprehensive data management platform Your instruments Your systems Your samples Your people NuGenesis 8 integrates them all That s data management eLab notebook and laboratory execution capabilities on a single platform Now science driven business minded organizations like yours can capture catalog access and deliver information with more insight than ever before Easy access to this insight means better collaboration And in the end greater results To experience the po
89. eature means users can control and monitor runs from their mobile device With remote monitoring and control of Optima XPN and Avanti JXN Series centrituges Users can ac cess the centrifuge from virtually anywhere via computer or mobile device using the MobileFuge app available for OS and Android devices With email diagnostic alerts lab managers are notified if their run needs attention Avanti Beckman Coulter and the stylized logo are trademarks of Beckman Coulter Inc and are regis tered with the USPTO The trademarks and names of other companies and products mentioned herein are the property of their respective owners Copyright 2014 Beckman Coulter life Sciences Life Sciences a www beckmancoultercentrifugation com May 2014 Lab Manager 85 86 PRODUCT IN ACTION A COMBINED METHOD FOR QUANTITATIVE AND QUALITATIVE CELL BASED RESEARCH Biochemical and cell based assays using a microplate reader provide quantitative data on ex vivo cell behavior while viewing cells with a microscope allows researchers to see cellular and intra cellular processes via fixed cells or with live cell imaging Both methods are equally important to life science research and the drug discovery process Together these methods provide valuable content rich data that otherwise requires the expense of multiple instrumentation The Cytation 3 Cell Imaging Multi Mode Reader from BioTek Instruments Inc combines both methods in
90. ed to test for antibiotics and if milk tests positive in this second test the load of milk is rejected and the lab work continues with further testing to determine which farm was responsible for contaminating the load of milk During processing the lab performs analyses on cheese and powder samples pulled from production www labmanager com J j Laboratory Information Bliss SmartEreezer The Single Solution for Automated 80 C and 180 C Cryo Storage JOOOT J Fur qin sr sa DoH For an efficient laboratory workflow PERSPECTIVE ON A FOOD AND BEVERAGE LAB 2 Lab tech Dan Tellock testing milk sample on milk analyzer and depending on the sample the lab can monitor the moisture fat salt content pH and or ash mineral content There 1s also a microbiology lab on site which is responsible for analytical and microbiological in process testing running standard plate counts and performing coliform yeast and mold analysis on all of the cheeses and powdered products A day in the life The lab has three full time and three part time technicians with Labelle filling in whenever necessary All of the technicians rotate responsibilities weekly moving between cheese lactose or whey concentrate product analysis and they can be given additional duties such as running milk samples environmental swabbing or taking air samples The plant is a 24 7 facility but the technicia
91. eese The laboratory is at the center of all the action in the facility which also produces lactose powder and a whey protein called reduced lactose Ivan Bartakovic BUSINESS MANAGEMENT 20 Marketing Your Lab As budgets for agencies tighten available grant money shrinks and money for lab testing becomes less of a priority it is increasingly important for labs to draw in new business to increase revenue While there is no quick fix labs must figure out who their potential clients are before trying to market their services Lynda Seeger LEADERSHIP amp STAFFING 26 Managing Culturally Diverse Teams Managers of R amp D groups in North America and elsewhere face multicultural situations with increasing frequency In those situations they must deal with the potential for multiple cultural clashes among team members The fusion approach is one way to mitigate such conflicts Peter Gwynne TECHNOLOGY 30 Document or Die Keeping track of everything is especially critical in the cell culture field in order to ensure that the data from cell culture research is accurate and to prevent incorrect information from being published Three cell culture professionals discuss documentation s importance in their labs how they do it and how they handle the challenges involved Rachel Muenz HEALTH amp SAFETY 38 Lab Manager Breath of Fresh Air Use of respiratory protection in hazardous atmospheres is the norm Ye
92. ell lines and they have been treated many different ways so we have a fairly elaborate tracking system for those he explains Otherwise 1n order to identify what a particular cell line is you d have to go back through the logbooks TECHNOLOGY essentially page by page and identify everything that s happened to that cell line to make it what it is Schwartz s lab s documentation process for its cell lines involves a nomenclature system that describes the history of each line from beginning to end along with a computer based inventory system with bar code labeling At Stamford for similar processes such as media for mulation or analytical methods the lab uses forms that are filled out by the scientist while logbooks are used for equipment such as pH meters to record the rel evant data Researchers use lab notebooks when they are developing or optimizing a process and for developed processes a Stamford scientist or engineer fills out batch records Zaidi says Stamford uses Excel spreadsheets and printouts from some of its analytical equipment and they record some data on computers but Zaidi says electronic technolo gies haven t taken over her lab I haven t found any electronic systems that can fully replace notebooks just yet particularly in a lab where development is being performed and activities can vary greatly she explains Reliable Versatility Expanded temperature ranges for laborator
93. en heating technology and the brains of our balances the patented MonoBloc weigh cell for speed durability and accuracy Pair this with the backing of the METTLER TOLEDO team the largest factory trained service organization highest rated technical support and our at the ready applications team and you ll experience why extraordinary may even be an understatement Visit us at www mt com na searchisover to learn more and challenge our moisture analysis experts 10 GOOD CHEMISTRY HEM BY BERNARD TULSI quick glance at the display screen of the average smartphone will provide clues about the ubiquity of the mobile app Talk to the phone s user and you will likely get regaled with accounts about the indis pensability of apps in the conduct of modern life With expanding roles in helping design experiments collect samples replenish consumables monitor projects access literature overcome software limitations and collate data along with many other functions apps are fast becoming indispensable in the laboratory also While definitive data are not readily available on the numbers of apps downloaded specifically for laboratory use it is a reasonable expectation that information technology savvy lab staffers were responsible for a sizeable portion of the 102 billion total app downloads in 2013 IT re search and advisory company Gartner Inc estimated app revenues at 26 billion last year 83 billion d
94. ence research the pipetting operations required to setup PCR can be quite monotonous for technicians conducting the process manually This can lead to procedural errors and inconsistencies not fo mention decreased time and energy for researchers to make valuable and intellectual contributions to their research labs Automating your qPCR sample preparations and other processes can happen right out of the box The intuitive qPCR assistant operates directly from the PIPETMAX touchscreen inter face allowing researchers to import sample information configure reaction proportions set up single or multiplex assays and even export plate files for common thermocyclers PIPETMAX does the rest by generating pipetting protocols based on your configurations and performs them quickly and efficiently Once you ve automated your qPCR sample preparation you can add additional kits that allow PIPETMAX to perform other processes such as Next Generation Sequencing ELISA nucleic acid cleanup and cell based assays You can also add the PIPETMAX protocol builder software to create completely custom protocols and import them into PIPETMAX for solutions that are unique to your needs With PIPETMAX the possibilities are endless APPLICATION NOTE CAB gene expression d js di a des i Mock treated dpi ii PVY RNA levels E PY treated PWYtreated Mocktreated Mock treated 4dpi Fpi dpi 7dpi dpi 4 Sample Analysis with qPCR a Expression
95. enerator that only produces gas when necessary and has a limited capacity labs can dramatically reduce both the explosive potential and the amount of time and money spent handling heavy dangerous cylinders Making the Helium Switch Make Sense Most laboratories are facing a future with a less reliable and more expensive helium gas supply causing them to explore their carrier gas options Large labs that wish switch to onsite hydrogen gas will have to invest in many small hydrogen generators for each lab creating upfront costs that can be tough to justify But hydrogen is quickly becoming less expensive than helium and is a more efficient carrier gas The lab server solution allows large labs to justity the switch while providing their practitioners with a carrier gas that offers superior coskeffective results A Proton OnSite hydrogen gas lab server can be installed into a large facility in hours ana are easier to maintain than several generators or rooms filled with heavy dangerous cylinders The lab server is the only option for large labo ratories that need pure and constant hydrogen gas in every room and on every floor Proton OnSite S Series Hydrogen Generator The Proton OnSite S Series hydrogen genera tion systems produce up to 18 8 slom of ultra high purity hydrogen gas for multiple use with multiole GC systems A single S Series hydro gen generator when installed into a large lab s gas systems will be able to serve n
96. entative processes We must emphasize that respirators only reduce the amount of contaminants within the breathing air but don t eliminate them altogether Each type of res pirator carries an assigned protection factor which can be considered the theoretical amount of protec tion a properly fitted respirator might provide The highest protection factor for APRs 1s only 50 accord ing to NIOSH APRs should be used only in relative ly low hazard areas where a single face respirator seal break might be unpleasant but not dangerous For high hazard areas where atmospheres are oxygen deficient or otherwise immediately dangerous to life and health IDLH the use of supplied air or SCBA is required Although one may still find some demand type units where negative pressure with respect to the outside of the mask is created when taking a breath these provide much less protection than the pressure demand units With pressure demand supplied air the space within the mask is maintained at a higher pressure than that of the outside air Thus if there is a break in the respirator seal air should rush out of the mask preventing the entry of any contaminants The use of SCBA should be required for initial entry into clandes tine drug labs due to the multitude and unknown con centrations of chemical hazards that may be present A few words on OSHA regulations regarding respira tor use The two basic requirements are that no one may
97. ere was a fire in a feed and fertilizer store in Gainesville Florida where the Safety Guys lived and worked Large quantities of smoke were generated and police were called in to assist with cordon ing off and securing the area Many of the officers who were stationed downwind ended up with some significant symptoms of chemical exposure through inhalation of the smoke from these agricultural products Some of our fire service personnel referred jokingly to these perimeter officers as the blue canaries demonstrating the boundary where use of respiratory protection was needed Shortly after this fire we were asked to come help set up a respiratory protection program for the police department To the best of our knowledge the department didn t have a single functional respirator available at the time of the fire Things have come a long way since then and use of respiratory protection in hazardous atmo spheres 1s the norm Yet there are still many misunderstand ings and misconceptions surrounding the use of respiratory protection whether it is in response to a possible chemical spill incident in the lab processing chemical wastes in con fined spaces or just dealing with routine chemicals in your normal activities So as they say let s begin at the beginning Respirators are designed to prevent contaminated air from entering the body There are several basic facepiece designs Typically one sees half mask respirators
98. esolves objects at submicron levels Raman is used for a wide variety of scientific problems from verify ing chemical composition and finding identifying con taminants in products to optimizing production processes According to Ellis the Thermo Scientific DXR x1 Raman imaging microscope is a new approach to Raman imaging It differs from traditional systems by working at the problem level that is an entire chemical image rather than depending on a user to work with individual point spectra to build an image up The DRXxi rasters images by collecting spectral data at a very high rate statistically processing and interpreting behind the scenes and displaying results during collec tion Color staining with chemical or morphological information reveals many dimensions of data Ellis says Chemical identification and distribution physical information such as materials strain and relative concen tration information all are conveyed visually The DXRxi supplements many microscopic techniques that provide visual or topographical information and that often require expertise subjective interpretation or additional measurements that must be correlated to the microscopic image to be meaningful More important the DXRx1 replaces traditional Raman microscopes by operating in an entirely differ ent way Ellis says by delivering meaningful chemical images nearly instantaneously It s really microscopy powered
99. essors Hemmig emphasizes We do have models that use them but we prefer to sell the ones without compressors because they ll work forever The key word in on site gas generation 1s reliability The alternative to compression is bringing in tanks of compressed air which Hemmig says is no big deal Companies are used to gas cylinders We believe a gas generator should be at least as reliable as a cylinder House air is also a possibility but users must first remove ubiquitous pump oils before allowing house air into generators Systems need to be as oil free as possible Matheson and other firms sell in line purifiers to remove oil in house air www labmanager com Together we can conserve non renewable helium by converting GCs to hydrogen Helium is a non renewable resource There is a global shortage of helium that has oe caused prices to increase significantly Many GC users are considering switching to climate control hydrogen as a carrier gas electromechanical Hydrogen provides many advantages over helium including higher resolution shorter run times longer column life and cost savings Many instrument manufacturers now provide resources to make switching carrier gas easier fluid amp gas handling hydraulics pneumatics process control sealing amp shielding A hydrogen generator is a safe convenient and inexpensive solution to storing hydrogen cylinders ENGINE
100. ethod Scouting System DE Nexera Bj BOOTH 85 e Capable of automatically investigating up to 96 combinations of mobile phases gradients and columns Improves HPLC method development productivity in both R amp D and QA QC environments Equipped with two pumps each with a quaternary valve which allows analysts to run binary gradients with 16 different solvent pairs Transfer program allows ultra high speed conditions to be transferred to conventional conditions Shimadzu www ssi shimadzu com Microscope for Correlative Raman SEM Imaging RISE hy s N e Combines confocal Raman imaging and scanning electron RISE y ea microscopy within one integrated microscope system LE i a IR e Can also generate 2D and 3D images and depth profiles e Enables for the first time the acquisition of SEM and Raman images from the same sample area and the correlation of ultra structural and chemical information with one microscope system www tescan com www witec de TESCAN WITec PRODUCT SPOTLIGHT A MAGIC WAND FOR MATERIAL ID HANDHELD RAMAN ANALYZER OFFERS SIMPLICITY AND CONFIDENCE TO USERS A highlight from Pittcon 2014 in the handheld Raman instrument world was Rigaku Raman Technologies release of the Progeny The company s latest handheld analyzer is designed to deliver comprehensive and accurate materials identification and improved ease of use adaptability and reliability Today is a pretty profound d
101. factor in the success of MS A key consequence of MS instruments getting smaller and more feature full but less costly has been the adoption of MS as a detector for high performance liquid chromatography HPLC and gas chromatography The mass detector Mass detectors for chromatography systems while not quite ubiquitous are steadily gaining ground in analytical labs particularly in regulated industries as a complementary detector to UV MS confirms the identity of peaks seen in ultraviolet traces and quantifies and identifies compounds lacking a UV chromophore MS has become the go to detector where science or regulation calls for lower limits of detection for impurities or target analytes The knocks against MS however have been high cost and its requirement of specialized expertise Waters Milford MA has recently introduced a new mass detector the ACQUITY QDa Detector for chromatography separations According to Howard Read senior product manager for mass spectrometry the ACQUITY QDa Detector was a response to customer needs particularly in pharmaceuticals May 2014 The driving trends here are risk mitigation quality management compliance productivity and cost control Read says We were responding to these ongoing needs of generating enhanced data for every sample analyzed particularly for laboratories that do not currently employ mass spectrometry Now they can make scientific decisions without h
102. fficulties of working with each other As the Americans saw it their French colleagues took an analysis paralysis approach to problem solving They insisted on analyzing the prob lem completely and correctly before taking any action Americans in the French engineers view insisted on ac tion from the start often at the expense of fully under standing the problem Cultural disagreements of that type aren t neces sarily insoluble When an American software engineer started to work with a team of Israelis for example he was shocked by their argumentative approach toward him until he realized that they took the same approach to each other He adapted by imposing some structure on the team s work while allowing himself and his col leagues to express themselves naturally In another case American and British members of a research team had violent disagreements over the speed at which they worked on a project the Americans wanted to go full steam ahead while the Brits wished to advance more slowly in case they met serious pitfalls Manage ment accommodated both groups by setting an in be tween speed that kept the project moving while allowing it to foresee problems And when a group of Japanese engineers encountered huge challenges cooperating with Indian engineers on a project for Infosys they organized some training materi als designed to stimulate the two groups to talk about their assumptions and experiences The materials hel
103. flowsciences com www GenTechScientific com www gilson com www helmerinc com www hettweb com www integra biosciences com www labanswer com www labconco com www labnetinternational com www megalabtechnology com www mt com www naosmm org www nuaire com us panasonic healthcare com solutions parker com conservehelium www photonis com www polyscience com www ProtonOnSite com www ruro com www sigma aldrich com www sonntek com www thermoelectric com www tecan com www thermoscientitic com ULVAC com www vistalab com www waters com www wheaton com PRE OWNED EQUIPMENT MARKETPLACE S High Quality T a COnQuel pre ownil SCIEN uu Instruments E T Mass Spec Chromatography Biotech General ConquerScientific com 877 9 CONSCI info conquerscientific com EQUIPNET www EquipNet com LMM 800 371 6555 APPRAISERS BROKERS AND AUCTIONEERS OF INDUSTRIAL EQUIPMENT Federals5 Equipmentzo Company Equipment Solutions for Pharmaceutical Laboratory and Research Industries 216 271 3500 www fedequip com pharmaceuticals fedequip com 8200 Bessemer Ave Cleveland OH 44127 GenTech Your Trusted Source for High Quality Expertly Refurbished Analytical Instruments Thermo Orbitraps 5975 7890 GC MS Thermo Vantage Thermo 150 Quantum Access More than 18 years of and so much mare Satisfied Customers gentechscientific coma bh CALI A AE E Lab Manager 97 PARTING POINTS
104. gelo adepalma com FOR ADDITIONAL RESOURCES ON LIMS INCLUDING USEFUL ARTICLES AND A LIST OF MANUFACTURERS VISIT WWW LABMANAGER COM LIMS May 2014 gt T Lab Manager 53 LIMS and The Mobile Lab Data at your fingertips How LIMS Are Enabling the Mobile Lab of the Future By Matthew Grulke Thermo Fisher Scientific magine that you re a field technician for a public water provider Your company is committed to supplying clean safe drinking water to millions of people so it has implemented a sophisticated water sampling program to ensure water is free of contaminants Across this water com pany s territory technicians just like you are collecting and testing samples from reservoirs water treatment facilities and even customers homes Today you re collecting samples from various points around a lake that supplies water to one of your company s main processing facilities After filling each sample bottle you transcribe its barcode into your notebook You check your watch and jot down the time You remember to add a preservative that ensures the sample gives accurate readings after it has been transported to the lab Finally you pull out a GPS unit and note your exact loca tion coordinates When you get back to the lab all of this information recorded manually will need to be re entered into a database and combined with the test results As you pack up your notebook and GPS i
105. h Jenny Meyer testing samples in microbiology lab pass on and issues are communicated by e mail If there are new requirements for analysis Labelle will hold an informal lab meeting to address the topic and discuss how lab workers can accommodate the requirements as a team and he will get immediate feedback from the technicians Labelle says that he does little supervising and acts as more of a resource for staff filling in for people who may be away or assisting them with any problems they may have Like many lab managers he is responsible for many duties with regard to the maintenance and operation of the lab Labelle is responsible for troubleshooting any equipment that breaks down and he performs preventive maintenance and calibration as required e g the calibration of the milk analyzer on a monthly basis His duties also include maintaining inventory ordering supplies and keeping the workers motivated Labelle enjoys working with and training his staff there is not a high turnover in the lab and the atmosphere 1s positive as all are happy to help out when they can He is available to his staff if there are any problems and the biggest challenge he faces is keeping staff motivated and excited about their work Labelle also says he enjoys the challenge of getting a piece of equipment up and running and he has learned to become self sufficient by doing his own troubleshooting and working with manufacturers over the phone
106. h your audience Once again look to your current client list and figure out what other similar businesses are out there If you have a large amount of potential clients located close to you consider holding an open house or lab tours and inviting these clients Con sider placing virtual tours or short educational videos of your services or your lab equipment on your website Networking with others in the sciences is another o good way to meet prospective clients As we all know Sample display marketing the importance of drinking water instruments break down or a project may require analy testing Source PHMDC Laboratory ses that a lab is not able to handle Those labs will need Well plate pipetting made simple Plate filling with repeat dispense Plate duplication and reformatting Interchangeable pipetting heads VIAFLO 96 384 Electronic hand held pipette m 96 and 384 channel pipetting as easy as manual single channel pipetting m Increased productivity due to a full range of pipetting modes including N E 5 R A repeat dispense serial dilute and customized programs Interchangeable pipetting heads allow precise pipetting between 0 5 and 1250 ul www integra biosciences com BUSINESS MANAGEMENT someone to do the testing for them and if they know you from professional meetings they may turn to you when they need help A less well known and underutilized method f
107. he time Organizations are structured to fail We make it almost impossible for some people to succeed One simple example which we can detail in another article is mixing short term and long term oriented groups under one manager Consider sales and marketing Mar keting is all about brands and getting someone to call for information down the road Sales departments are all about closing new business today As the end of a quarter ap proaches do you think the manager is thinking about next year No way S He is focused on making the cur rent sales quota More importantly most lab opera tions need to understand the differ ence between leaders managers and doers This lack of understanding is the reason so many labs are struc tured around people based on their longevity and personalities instead of their performance At the most simple level lead ers set direction They want to be loved or followed because people buy into their ideas They have vision and the conviction to lead a company into the future nager Academy ORGANIZING PEOPLE By Steve A Epner Managers like to get things done through others They do not care what anyone thinks of them They plan assemble teams and execute in a way that will move the organization in the direction set by leadership Good leaders love strong manag ers even if most could not work for one Strong managers appreciate strong leaders who give
108. ification of trace synthetic cannabinoids within complex biological matrices with excellent sensitivity and specificity compared with standard methods GenTech Scientific is the exclusive U S distributor for the ID Cube and ASAP DART Sources and a provider of complete ID CUBE equipped MS systems in other countries www labmanager com Maximizing Reproducible Biological Sample Prep Routine pipetting tasks across a larger number of samples can often be inef ticient complex time consuming and expensive These hurdles can lead to increased training requirements preparation time procedural errors and ulti mately hold back the pace of your experiments When preparing biological samples you need an assistant you can trust and PIPETMAX is the ultimate lab assistant focused on consistency Maximize qPCR assay reproducibility using PIPETMAX Download the full application note http www pipetmax com pergpcr PURPOSE e Maximize qPCR sample prep accuracy and eliminate inherent variability e Enhance sample purity RESULTS e Gene expression of CAB was greater in PVY infected plants than in healthy mock inoculated plant leaves e PVY viral RNA levels increased gt 15 fold in 3 days demonstrating a rapid spreading of the viral infection e No PVY expression was detected in mock inoculated plants no contamination e Enhance sample purity Though it s a tundamental process that enables most life sci
109. ing that requires a specific temperature you need a reference to check it against That way you can verify the operation of your instrument People want to take traditional methods and make them faster Newer technology can also be more accurate For example Anton Paar offers a frictionless technology that provides more accurate results Today s viscometers can also be easier to use As an example McGregor points out the touchscreen interface You can use it like a handheld device he says It also provides the ability to see live graphical information in terms of what s being measured In many cases companies can save money by placing a viscometer in a production line making measurements in real time This way says McGregor they can monitor viscosity continuously and make process changes if they see deviations When shopping for a viscometer McGregor encourages customers to try to do what others are already doing because they ve established test procedures on known parameters That way you can find the right instrument and figure out how to use it without starting from scratch Mike May is a freelance writer and editor living in Ohio You may reach him at mike techtyper com FOR ADDITIONAL RESOURCES ON VISCOMETERS INCLUDING USEFUL ARTICLES AND A LIST OF MANUFACTURERS VISIT WWW LABMANAGER COM VISCOMETERS 52 Lab Manager May 2014 www labmanager com PRODUCT FOCUS LIMS C
110. isciplinary teams the sooner the work that we all do could become more efficient dynamic and ultimately of higher quality Mark Lanfear is a global practice leader for the life science vertical at Kelly Services a leader in providing workforce solutions He has operated clinical trials around the world for almost two decades In addition Mark is a featured speaker at many life science industry conferences and a writer for life science peri odicals He can be reached at MARL773 kellyservices com or 248 244 4361 www labmanager com There s a new kid in town As a food analyst you know the food we eat must be safe From matrix processing to quantification let Sigma Aldrich be your partner in ensuring a safe and healthier world Solutions include Standards amp CRMs e Vitroids Microbiology CRMs e Species Identification e Gluten Testing e HybriScan Pathogen Detection Kits Pesticide Residue Analysis e Mycotoxin Testing For a limited time request a free sample of Vitroids bacterial standards for microbiology testing at sigma aldrich com vitroids Get to know us at sigma aldrich com food 2014 Sigma Aldrich Co LLC All rights reserved SIGMA ALDRICH is a trademark of Sigma Aldrich Co LLC registered in the US and other countries Vitroids and HybriScan are trademarks of Sigma Aldrich 81975 SIGMA ALDRICH 18 Lab Ma T i y e s a consultant and professor I see it all of t
111. ishes Researchers are using this technique to inves tigate biomarkers and the effects of drug treatments among microbial colonies to identify natural products www labmanager com E INSIGHTS ON IMAGING SYSTEMS a for drug discovery and to visualize downregulation and upregulation of genes You can tell if transfection worked by imaging regions where proteins encoded by that gene are expressed Goodman says LAESI enhances the capabilities of conventional medi cal imaging techniques as well In September 2013 Protea entered a collaborative research agreement with Virginia Commonwealth University s Center for Molecular Imag ing The collaboration will combine LAESI with the uni versity s in vivo molecular PET SPECT and MRI MRS medical imaging capabilities to investigate the molecular basis of cancer Alzheimer s and other human diseases MS has brought about changes in imaging based diag nosis of tissues that microscope based imaging could not For example researchers from Imperial College London use MS data from a tissue sample to create an image of the sample s chemical composition The technique maps chemical components of interest and may help medical researchers differentiate tissues that appear similar under ordinary light microscopy For example mass spectral im aging could indicate differences between cancer subtypes This advance pushes the limits of conventional pathol ogy histology which over th
112. it s important to select the right unit to ensure it meets the necessary requirements With low profile units ideal for viscometers to large refrigerated circulating baths with unlimited programming for accelerated bever age testing and an extensive list of additional tactory installed options PolyScience offers products that meet almost any need E E www labmanager com PRODUCTS IN ACTION PROTON ONSITE HYDROGEN GENERATION SYSTEMS The Proton OnSite line of hydrogen generators is the simpler more cost effective and less complex approach to supplying hydrogen for laboratory and scientific applications Offering units that produce from 300 cc min to 18 8 slpm of gas these generators use Proton OnSite s patented Proton Exchange Membrane PEM electrolysis tech nology to produce gas at 99 99995 percent purity without the need for high pressure liquid hydrogen tanks or compressed gas storage It s the safe cost effective solution for any lab Serving Large Labs with a Complete Hydrogen Solution Many lab managers understand the value of switching from delivered gas to an on site hydrogen gas supply On site generation offers a pure constant and safe supply of hydrogen gas as opposed to delivered cylinders But for large laboratories that cover multiple rooms and floors switching to hydrogen gas genera tors is a tough decision A large lab may have tens or hundreds of Gas Chromatography GC systems whi
113. ji 200 PROG 4 4 WIL2 5 200 PROVGCM gt Figure 2 ADCC Reporter Bioassay conducted using the Infinite 200 PRO Reader with GCM 200 PRO GCM offers equiva lent performance to assays incubated in a aH 4 Fold Induction f E i i id M f n standard tissue culture STC incubator Log Control Ab Anti C0203 g ml Log Control Ab Arti CO20 g ml Results The results of the ADCC Reporter Bioassay with Raji or WIL2 S target cells were comparable in both told induction and potency whether the incubation was performed in an Infinite 200 PRO with GCM or in a standard tissue culture incubator figure 2 The potencies obtained were 66 ng ml and 78 ng ml respectively for Raji cells and 36 ng ml and 37 ng ml respectively for WIL2 S cells O AN Conclusions The Infinite 200 PRO with GCM is functionally equivalent to a tissue culture incubator for the re www tecan com porter induction phase of the ADCC Reporter Bioassay This study showed that the convenience Call The Americas 1 919 361 5200 of using a kit containing frozen thaw and use cells was further enhanced by the use of a reader Europe 49 79 5194 170 that enabled both temperature and gas control effectively mimicking the conditions provided by Asia 81 44 556 731 a standard cell culture incubator into tecan com Lab Manager May 2014 www labmanager com PRODUCTS IN ACTION Streamlined workflow expanded selectivity and a
114. jor manufacturers And with the CrossLab Stand Behind Warranty you get confidence not compromise The Meajsure of Confidence Learn why we should be your trusted source at WARRANTY Agilent SUPPLIES SERVICES D SOvation m Performance ergonomics and value in one elegant design A Our new Ovation M combines the revolutionary ergonomic performance of the Ovation design with a conventional analog volume setting capability lts designed for the way your hand works which means no sore thumb no tired arm no aching neck Plus its contoured shape and adjustable hook offers a custom fit for every user in the laboratory The Ovation M might just be the most comfortable easy to use pipette you will ever experience with all the accuracy and versatility your work demands Get all the details from your Rep visit www vistalab com or call us at 888 652 6520 WistaLab Dispensing Dispensing With Tradition Ovation keeps your wrist in VistaLab Technologies Inc dl position 2 Geneva Road Brewster NY 10509 USA recommended by ergonomists
115. king in a more interdisciplinary fashion This involves knowing that different perspec tives on a single project are the key to in spiring new ideas A so called common front of different perspectives from team members will most certainly take a project to a higher level And if manag ers want their team to think in a more interdisciplinary way they must first be willing to do it themselves Also important is for a manager to be an effective enabler of cross disciplinary work It s not enough to simply put ten experts in a room and expect them to spontaneously work together toward a goal Communication is key keeping the big picture in mind and letting the team know throughout the course of the project that it is critical to work together and share ideas Be sure that everyone understands the cross disciplinary na ture of the proyect and that they are no longer working separately but to gether Make sure they know how the work of the other team members may affect their own And make sure they know the importance of this new type of interaction between their colleagues It may seem as though a spirit of work ing together would happen naturally but the manager of a project must ac tually take deliberate steps toward this goal especially with professionals who are so used to working on their own The old way of working in silos is clear ly gone The sooner managers are able to effectively lead cross d
116. l lab layout features a number of function based stations including weighing media preparation pH meters centrifuges and colorimeters among others Re searchers and technicians generally visit and use the tools at these stations and record data in paper lab notebooks The replacement of the paper notebooks with smart mobile devices equipped with specific apps enormously increases efficiency accuracy and the ability to interact on a real time basis with a larger network Among other benefits this also reduces transcription errors because unlike with paper records technicians need to input data only once into mobile devices When the time and location where a sample was col lected are important such as in wastewater monitoring capabilities embedded in mobile apps running on a smart phone could store the time the sample was taken and the GPS coordinates of the location This information can then be uploaded into the laboratory information management system LIMS and become available across the enterprise Security and compliance such as avoiding the down load of extraneous noncompliant data or the unauthorized or inappropriate release of critical sensitive results are among the major benefits end users derive from the de ployment of smart apps Apps that alert researchers about the qualification status of instrumentation or the need to obtain standards or recalibrate equipment also help ensure both compliance and good la
117. le automated nanoliter scale assays require as little as 1 uL of sample M e Ready to use kits are fully qualified for reliable use in clinical research nmh 4 e Accuracy and precision meets or exceeds ELISA performance e Enable clinical researchers and drug developers to generate valvable research data much faster than typical ELISA kits EMD Millipore Y M www emdmillipore com Lab Manager May 2014 Total Phosphate Field Kits CHK8540 e Comes in a cardboard box and contains everything needed to perform 50 tests 50 ampoules sets oxidizer powder neutralizer solution stabilizer solution reducer powder oxidizer powder dispenser cap 3 mL syringe with tip tip breaking tool funnel and instructions e Require the use of a digester block and the V 2000 photometer or a spectrophotometer capable of accepting a 16 mm diameter round cell Environmental Express www envexp com Raman Spectroscopy amp Imaging Software Lambdafire R e Written specifically for Raman microspectrometers running Window 8 e Designed to collect analyze and process both Raman microspectra and images from CRAIC Raman microspectrometers e Suited to both industrial processes and scientific research e Simple to use yet contains many advanced spectroscopic imaging and data analysis features CRAIC gt www microspectra com New GC Software Functions mE GC Image Bl MS BOOTH 107 e New functions make use of JEOL s AccuT
118. manipulation or INTEGRA www integra biosciences com Water Purification System Cartridges Part D400377LS e Economical replacement water purification cartridges suited for Thermo Scientific Barnstead Mega Pure deionizer accessories e Suitable for operation in the Mega Pure single D1 or dual D2 cartridge deionizer systems and the MP 12A glass stills e Enhance the performance of Mega Pure glass stills by reducing scale buildup and increasing distillate purity LabStrong www labstrong com Externally threaded 3 00ml Tubes e Feature a unique 2 dimensional Data Matrix code laser encrypted on the tube bottom and large pe human readable code on the tube sidewall E pl Provide easy and unambiguous identification and r T 4 storage of donor and transplant samples g 2D code identifies the sample its coordinates within S a 24 tube storage rack the particular rack and the location of the rack in user s freezer www micronic com Micronic May 2014 Lab Manager 75 SURVEY SAYS Types of cold storage used by survey respondents Blood bank and plasma 6 Explosion proot 7 Flammable materials storage 6 Upright general purpose lab freezers 26 Under counter general purpose lab freezers 10 Low temperature chest lab freezers 6 Low temperature upright lab freezers 14 Upright ultra low temperature freezers 17 Chest ultra low temperature freezers 5 Other 3 For service and repair survey respondents report
119. manufacturing need to be properly documented she says In the case of our preclinical and diagnostic protein production customers proper documentation www labmanager com Ultra Low Freezers sure we could have designed an Ultra Low Freezer like everyone else But we didn t That s not our way We believe in starting at the beginning finding the best most forward looking way to design and engineer each component and using manufacturing techniques that ensure quality results Why do we care Because How It s Made Matters TrueBlue www helmerinc com lt gt Helmer SCIENTIFIC 2014 Helmer Scientific All rights reserved ensures the ability for Stamford Bioprocess to repeat the process consistently This ensures that product quality doesn t change from batch to batch How it s done The way documentation is carried out varies from lab to lab but most use a combination of hard copy note books and files and computer systems It also depends on what exactly is being documented For most of the reagents we keep a logbook where we simply put a hard copy of the certificate of analy sis and data sheets associated with a given reagent Schwartz says adding that reagents are organized by type Along with those reagents and supplies used Schwartz and his team must keep track of the many cell lines they generate which involves another approach There are many different kinds of c
120. n collaboration to announce Mar 4 The company has partnered with the U S Pharmacopeial Convention USP to create a beta version of the USP Spectral Library for raw material identification in the food and drug industries A It s really a huge effort in terms of a collaboration to make this concept a reality said Ed Zhao USP s vice president of business development and allied compendial programs The library powered by Bio Rad s KnowItAll software technol ogy platform allows manufacturers to confirm the identity of active pharmaceutical ingredients and excipients used in their products during routine quality control measures The new tool also has applications in quality standards development and rapid screening in the field for counterfeit and sub standard foods and medicines The USP Spectral Library has the potential to help ensure the quality of medicines and foods throughout the world Zhao said m Metrohm USA s new 940 Professional IC Vario high performance ion chromato graphy system The USP Spectral Library for raw material identification in the food and drug industries is powered by Bio Rad s KnowltAll software technology platform 63 Texas Instruments DLP NIRscan evaluation module for spectroscopy is built by Keynote Photonics www labmanager com Our Mission is to support the professional development of Scientific Laboratory and Materials Managers
121. n what biological matrix was used when the analysis was done the instrument it was done on and the name of the analyst You are just creating areas on the database that you can then control and as the database grows you can use all this information to do a very specific search We are happy with the speed with which we can search However there is a lag when you work directly with the remote database So we first have to create a local database as a part of our workflow The idea is to do all the work locally and then export the data to the remote database at the end of the day or when the job is done Q Is the database fairly intuitive or did you have to undergo extensive training to use it As The first criterion was the ease of getting data in so the intuitive nature of the software was a pri ority However ACD Labs did provide the necessary training and we have had subsequent training on the various releases of the software as it has evolved We tend to run with our core users who are more experienced in using the database and for our part time users who use the database for some spectral identification we have our in house resource work through any issues with them We have training manuals and user guides online that people can use allowing most problems to be very easily resolved Q How do you justify the return on investment for this database A The thinking we have embraced to justify our investment is to remind
122. n and about ten times the www labmanager com sensitivity of UV Higher end triple quad MS provides the quantitative precision of a UV detector below 2 percent and sensitivity close to the LC lower limit The emergence of alternatives to MS for companies that rely on high sensitivity and ultralow limits of detection could somewhat deflate the market projections cited earlier In other words all is not lost for chromatography labs that cannot afford MS Sensitivity is constantly improving for ultraviolet and photodetector array PDA detectors whose flow cells are evolving toward lower volume lower dispersion higher sensitivity detector cell designs For analytes lacking a UV chromophore such as sugars and most amino acids evaporative light scattering ELS is becoming more prevalent and is sometimes used alongside MS detection ELS 1s sensitive and provides an important alternative to the older refractive index detectors which cannot be used with gradients Weisenseel says LAESI solution to sample prep woes A relatively new technique is changing the way mass spectroscopists view sample preparation LAESI laser ablation electrospray ionization is a variant on standard electrospray ionization ESI long considered a gentle ionization technique for analyzing large delicate biomolecules by MS LAESI performs direct extraction and ionization for stand alone samples or for profiling the distribution of biomolecules
123. n orthogonal technique for your laboratory Giving you the power of normal phase LC but with the ease of use of reversed phase LC con vergence chromatography is a broad based complementary analytical platform that is tak ing its place as an essential separation technol ogy for modern laboratory analysis Bringing usability to gradients and chiral compounds With the power to separate both chiral and achiral compounds as well as utilize gradi ents this technique provides another reliable choice as an analytical plattorm that is orthog onal to reversed phase LC while significantly streamlining the entire analytical workflow In convergence chromatography the separo tion is achieved by manipulating the density and composition of a supercritical fluid based mobile phase Because of the very high dit tusivity of the mobile phase high separation efficiency and fast gradient formation can be achieved Additionally the diversity of station ary phase and mobile phase co solvent op tions provides access to the largest selectivity space available to any separation technique Assay of dapsone tablets USP method using UPC ing ACQUITY UPC b Biss a 1 EH EE 2 00 1 7 r LEC at bob biuh bigo ELD Diverse applicability and selectivity Convergence chromatography utilizes the same eluotropic spectrum as normalphase LC The flexibility of this technique however also allows the use of some conventional re
124. n reach him at angelo adepalma com May 2014 Lab Manager 67 PITICON ROUNDUP Y 68 n addition to the products introduced at Pittcon 2014 we ve already profiled in our Technology News section there were plenty of other exciting developments to share from the press conferences we attended in Chicago in March One standout from the show was the number of partnerships announced between manufacturers as well as lon ger standing collaborations that have generated some new technologies For example Beckman Coulter Life Sciences announced Mar 4 that it will be partnering with Wyatt Technology The collaboration combines Wyatt s expertise in protein characterization light scattering and biophysics with Beckman s know how in particle counting particle characterization and cell viability measurement and will help both companies expand in the particle market Beckman will also be manu facturing AB SCIEX s CEST 8000 system for biologics characterization a technology that resulted from AB SCIEX combining the CE business of Beckman Coulter with Eksigent nano and microscale chromatography to form a separations business called SCIEX Separations The CESI 8000 system took bronze in this year s Lab Manager May 2014 PARTNERSHIPS Collaborations between companies and organizations a trend at this year s show by Rachel Muenz Pittcon Editors Choice awards for most innovative product at Pittcon Another
125. ng points for the CLARIOstar s LVF Monochromators With further optimization the same percent change is seen figures table making it an almost seamless transition to a higher through put method without buying new filters References 1 Comley J Monochromator vs Filter based Plate Readers horses for courses or a win ning combination Drug Discovery World Fall 2007 htto ddw netgenie co uk en abling_technologies 347427 monochro mator_vs_filterbased_plate_readers_horses_ for_courses_or_a_winning_combination tml 2 Padilla Parra S et al Quantitative compari son of different fluorescent protein couples for fast FRETFLIM acquisition Biophys J 2009 Oct 97 8 2308 76 BMG LABTECH The Microplate Reader Company E J Dell Ph D BMG LABTECH GmbH 1 877 BMG LABS www bmglabtech com May 2014 Lab Manager 87 PRODUCTS IN ACTION 88 DART Technology for Cannabinoid Screening Rapid Identification of Synthetic Cannabinoids in Herbal Samples via Direct Analysis in Real Time Mass Spectrometry R A Musah M A Domin M A Walling and J R E Shepard Rapid Commun Mass Spectrom vol 26 no 9 pp 1109 1114 2012 Introduction Dozens of synthetic cannabinoid analogs purposefully meant to circum vent legal restrictions associated with controlled substances continue to be manufactured and promoted as producing legal highs These de signer drugs are difficult to identify in conventional
126. nic Lab Notebook 1 How local are resources and how available are resources for deployment training and extensions What is the timeline for availability and cost 2 How easy is it to extend the application Does it require IT or super users How long does training take to make modifications and how extensive is the API for modifications 3 How easy is it to get data back out of the system Is all information indexed and searchable Can users query and combine data from multiple experiments not just return a list of experiments What is the typical number of hours of admin time required to upgrade for a major release and a minor release 5 What level of support is offered How many support staff are there where are they located and what language do they support How is the support rated by other customers 6 Is your IP system safe in their system What is the chance the company will be around in five years What is the chance that the company will switch technologies and force an expensive migration What credibility does the company have in the past for delivering robust scalable secure and 21 CFR Part 11 compliant systems TOP 10 FEATURES FACTORS respondents look for when purchasing an ELN SECURITY 79 eC te EASE OF USE 72 BEME PRICE 66 E OS gt OOO OOOO OA innatas VERSATILITY 53 an SERVICE AND SUPPORT 53 Sn UP TIME 49 o APM CUSTOMIZATION
127. nnections must be made True col laboration may arise organically and in surprising ways with no plan But peo People who are used to working in formerly isolated departments may not know how to adapt What changed this dynamic in the life sciences and continues to inform the evolution of the workplace was the need to become more efficient Huge corporations basically needed to be more cost efficient and they realized that collaboration and the breaking down of the traditional silos would help them do that A welcome side effect is that we re all learning how to collaborate better with colleagues who may approach their disciplines very differently but who have the same goal We all want to develop bet ter products and tools and sharing our knowledge helps us do just that For managers this new dynamic has been especially challenging The ques tion remains How do you effectively manage cross disciplinary teams Sur prisingly there is little research out Lab Manager May 2014 ple who are used to working in formerly isolated departments may not know how to adapt at all leaving group dynamics on a cross disciplinary project open to negativity and stifling knowledge flow In this situation managers must take les sons from the general playbook for all new workplaces that are being informed by a need to work in a more cross disci plinary mode It is critical for instance for managers themselves to start thin
128. now singing the praises of hydrogen and are helping customers switch over from helium Companies like Shimadzu Thermo and Agilent are blazing a trail for us Several vendors including Agilent provide hydrogen leak detectors In the past year Parker has launched products with higher flows and pressures greater reliability and interconnectivity which help labs replace cylinders with generators The company has also innovated with system software that allows load balancing which benefits primarily high volume users Load balancing allows multiplexing up to 32 hydrogen generators into a single manifold with simultaneous independent control If one generator fails the remaining units pick up the slack to rebalance back to the original ageregate production rate No generator works harder than any other The ongoing helium shortage has created a robust market for hydrogen in gas chromatography Load balancing also enables control and performance monitoring of a network of hydrogen generators through a USB connection to a computer Angelo DePalma is a freelance writer living in Newton NI You can reach him at angelo adepalma com FOR ADDITIONAL RESOURCES ON LABORATORY GAS GENERATORS INCLUDING USEFUL ARTICLES AND A LIST OF MANUFACTURERS VISIT WWW LABMANAGER COM GAS GENERATORS 44 Lab Manager May 2014 www labmanager com PROTON THE LEADER IN ON SITE GAS GENERATION THE LAB GAS YOU NEED UN SITE amp
129. ns for example multicolor FISH A variation on this theme is two pho ton or multiphoton fluorescence Bruker s Billerica MA acquisition of Prairie Technolo gies last autumn afforded Bruker an entry into multi photon fluorescence microscopy a microscopy imaging technology in which Prairie was a pioneer In traditional fluorescence the excitation energy is shorter in wavelength and higher in energy than the emitted fluorescence In multiphoton two or more photons of longer wavelength lower energy strike the target simultaneously producing an emission of higher energy than the excitation does Lab Manager May 2014 A Stereomicroscope SMZ25 Nikon Instruments www nikoninstruments com Bruker also gained entry into confocal microscopy a high resolution high contrast imaging technique that employs point illumination and pinhole masking to eliminate out of focus light Although the combined excitation energy of the two photons may be the same or higher than in single photon fluorescence the impact on living organisms 1s gentler Think of being hit by two tennis balls traveling at 25 miles per hour versus one ball at 50 miles per hour Consequently dual photon fluorescence is ideal for imaging live cells that are able to withstand just so much excitation Cells survive longer so analysts can extract more information from them Multiphoton fluorescence enables exotic sounding ex periments such as uncaging op
130. ns start the day at five o clock in the morning The first technician to arrive gathers samples that need to be sent out for external lab testing whether they are samples from farmers for somatic cell and or antibiotic testing or environmental swabs or powder samples for pathogen testing The technician also generates reports for production reporting which products need retesting and which products are ready to be released for shipping The remainder of the shift is spent performing routine analysis on the cheese and whey and entering the data from the analysis into Oracle HETTICH _ MADE IN GERMANY Find out more by visiting The next technician who arrives works in the microbiology lab and is responsible for protein analysis ash testing and pH testing and running all of the microbiological work for production on that particular day Testing starts with ensuring that the milk is suitable for the cheese being produced To help production workers standardize the milk the technicians run the samples milk and cream through a milk analyzer To ensure that the milk is producing a high product yield vat samples made that day are run through the milk analyzer so that the lab can keep track of butterfat and protein for that day s production The results are provided to the production staff and the milk is adjusted accordingly To ensure that the consumer receives the best quality product the laboratory tests the product as it mo
131. o conduct a richer peer review Q So is it easy to share the data using this database and how secure is it A Data security is taken care of by our IT person nel and we have a licensed access to the database from an Oracle based server that has a firewall If you need to share your data with external custom ers such as contract research organizations CROs you need to put special procedures in place You could sanitize a certain space in your database and give the external customers access to it so that they don t get access to your entire database although that s not something we do here Q Is there something that is lacking or can be improved upon As The ability to share our data with the rest of the organization still requires licenses to the software This is currently changing soon anybody in the com pany who could benetit trom the data can get ac cess to it Just as the power of the biotranstormation map that links the parent molecule to its metabolites works for us can see other groups such as degra dation chemists benefitting from this as well They also have clusters of molecules where the active in gredient is broken down over time or by the environ ment so they would benefit from a similar approach Q Were you able to customize the database to fit your needs As It s almost scarily flexible You have carte blanche to rename bits of metadata including what species you saw the chemical structure i
132. oes for automated alerts on sample runs Mobile devices linked to the LIMS allow users to make a decision from a remote location about whether an outlying result requires a retest or a full investigation preventing unnecessary delays Because of these visualization and connectivity advances laboratory person nel are free to be far more creative as they design workflows Data management is no longer a limiting factor in the laboratory instead it is a driving force for innovation Learn more about LIMS and The Mobile Lab Matthew Grulke is the Director of Product Development and Technology for the Informatics business at Thermo Fisher Scientific 56 MILK TO CHEESE STARTING AT THE FARM THIS LAB KEEPS AN E ON QUALITY BY IVAN se w y PEA AS e ituated in rural Weyauwega Q isc the Agropur Dairy Cooperative plant continues the rich cheese making tradition of the state through its production of both cheddar and feta cheese The laboratory is at the center of all the action in the facility which also produces lactose powder and a whey protein called reduced lactose The laboratory is involved in every step of production starting with testing samples arriving from the farmers fields continuing through production and finishing with releasing the product for shipment to customers Jerry Labelle the lab supervisor at the facility explains The primary function of the lab is testing ingredient
133. of CAB gene normalized to COX and EF in infected compared to non infected mock inocu lated plants b levels of PVY RNA in samples P GILSON Gilson Inc PO Box 628098 Middleton WI 53562 P 800 445 7661 Web www pipetmax com May 2014 Lab Manager 89 PRODUCTS IN ACTION Automate your multichannel pipettes Multichannel pipettes are invaluable when working with multiwell plates However prolonged and repetitive pipetting sessions bear the risk of strain and fatigue offen resulting in repetitive strain injuries RSI and less reproducible results VIAFLO ASSIST together with INTEGRA VIAFLO II elec tronic pipettes form the perfect symbiosis to prevent users from RSI and to significantly increase the reproducibility of prolonged pipetting protocols such as serial dilutions plate filling and reagent addition By mounting a VIAFLO II electronic pipette on the VIAFLO ASSIST the pipette s protocols can be carried out fully automatically TYPICAL APPLICATIONS Sera Diyus Se al dilutions can be tedious due lo the man miing steps hal are required VIAFLO ASSIST caries oul pour serial dilubon protocols sutom bcad Samp ceding the number of mong Cres aperd ond ma volume Denai Guten M offen Chine cal with he piai in poria onematon Tras sows mote samples lo Se dlideda VIAFLO ASSIST allows Wg m landscape and porra formal The conceniated sample can be Gyan from a reagent ren reos a POR tube ship and the plate dga
134. oid can be performed for both blue live cell and green dead cell fluorescence channels using Gen5 Quantitative data presented in Figure 1D indicates that while the total number of cells counted in the spheroids is consistent across all diclofenac concentrations increasing toxicity is evident across the concentration profile of diclofenac reaching almost 100 percent toxicity at 300 uM Fluorescence microscopy and the image analysis capabilities of Gen5 provide multiparametric data that allows for a complete understanding of long term diclofenac toxicity on spheroid microtissues For more information visit www biotek com Lab Manager May 2014 j ao F Ej j i i aza E i di te os dan A p i d ag E y i E lt B F BE Soha JOE 3 lt C a a E MM Col Coura i 1 Ea Cia Cala 3 150 F 1004 a y i sod l lt D Dec borer uM A Figure 1 Images and image analysis of liver microtissue spheroids after 10 day incubation under several doses of diclofenac A 10x images of spheroids showing live blue and dead green cells B Mean green fluorescence is computed from each pixel of the 16 bit CCD camera green blue fluorescence ratio is computed from mean fluorescence intensities of each channel C Spheroid size is the diameter of the spheroid and spheroid area represents the area drawn by Gen5 around the spheroid D Total cell count represents the total number of cells in the field of view
135. on Microscope Tecnai Femto FEI www fet com FEI has continued its innovation in visualizing ultrasmall ultrafast events with its late 2013 introduction of the Tecnai Femto ultrafast electron microscope The device allows observation of events occurring at the atomic and molecular scale within time frames of femtoseconds 10 1 second These include the absorption of light energy and its transformation into heat mechanical changes through photoactuation and crystallization Tecnai Femto is the first system to commercialize ultrafast electron microscopy technology pioneered by Nobel laureate Prof Ahmed Zewail at the California Institute of Technology David Flannigan PhD a former Zewail lab member and now assistant professor of materials science at the University of Minnesota has noted that over the last decade microscope manufacturers like FEI have devel oped instruments that have made observations of objects as small as individual atoms relatively routine Ultrafast electron microscopy now gives us a powerful tool to look at the movements and changes that occur at this scale Because the distances are so small the time scale is also condensed it doesn t take very long to travel a nanome ter or two Using single electron pulses we have measured changes over time periods as short as tens of femtosec onds those are millionths of a billionth of a second Angelo DePalma is a freelance writer living in Newton NF You ca
136. or Promotions See if the company you are buying from has any tradein promotions or other discounts PolyScience will take in your old used cir culator regardless of condition and brand and will give you a trade in credit towards your new purchase All recoverable materials such as steel copper plastic and packaging will be recycled and refrigerants reclaimed Poly Science also offers discounts for educational facilities and volume purchases Consider Maintenance Like any other piece of equipment temperature control products require maintenance Be sure to set up a maintenance schedule or at least think about it before buying the unit so you ll know what it entails Select PolyScience Circulators include a Service sub menu tracks operation statistics and even notities you when your filters or fluids need to be replaced Accessorize Pick up accessories at the same time to save on shipping and downtime PolyScience offers tubing fluids and other accessories re quired to make the most of your new purchase PolyScience also offers an iOS app called Poly Temp Remote which allows you to moni tor and set the temperature of Circulating Baths equipped with Advanced or Pertormance Se ries Controllers This is ideal when the bath is located under a fume hood or you are offsite and want to check the status of your Circulator Conclusion Each application has a different set of require ments so
137. or advertising your lab services 1s offering to speak to groups about topics relevant to your work Speak at board and council meetings about concerns in their districts For example if spring flooding is an issue 1n your area prepare a short presentation about the impact of flooding on drinking water quality and the importance of testing Many civic groups like the Rotary or the Lions Club have guest speak ers Members of these groups are often local business leaders who at some point may need your lab services Use these groups as a way to talk about testing that could be done while pre senting information on topics of local concern Also don t forget about youth groups such as the Boy Scouts Girl Scouts 4H and Future Farmers of America These are your future clients Use the opportunity to educate these youth while promoting your business Consider placing virtual tours or short educational videos of your services or your lab equipment on your website Lastly whether your future clients are busi nesses or private citizens don t underestimate the use of the media Don t be shy about brag ging about your successes and drawing atten tion to your lab services Contact the media with potential stories when your lab helps in a newsworthy situation Don t be afraid to write articles for local newspapers magazines com munity newsletters or trade journals Sometimes even letters to editors about specific topics can
138. oton systems reach five times as deep to 1 5 millimeters Through the use of clearing agents that render opaque tissues clear scientists have achieved imaging depths of eight millimeters www labmanager com INSIGHTS ON IMAGING SYSTEMS DON T FORGET INDUSTRIAL IMAGING Industrial imaging includes nondestructive macroscale techniques for visualizing defects composition and oth er relevant features of materials or structures In keeping with the spirit of this article we will limit the discussion to microscope based imaging for which several dozen optical and nonoptical techniques exist FEI Hillsboro OR focuses mostly on scanning elec tron microscopy SEM dual beam focused ion beam SEM FIB SEM and transmission electron microscopy TEM Through its acquisition of Germany based Till Photonics FEI has recently invested in light microscopy as well In early 2014 FEI announced that it had acquired Australian firm Lithicon which provides pore scale micro computed tomography uCT or microCT equip ment to oil and gas companies A significant segment of FEI s business involves atomic scale imaging which encompasses molecules as well as at oms Relevant instrumentation includes TEM and scanning transmission electron microscopy S TEM TEM uses a focused beam smaller than the atoms themselves to sample along a group of atoms Bert Freitag director of product marketing for FEI s materials science business unit com p
139. ourselves that this ap proach could save an error from being made or stop an interpretation from being wrong If clinical trials go wrong because an incorrect interpreta tion was used then the ramifications can be se vere This database is a rich resource that can link up our organization it can save us from suffering the possible negative outcomes from continuing to rely on human memory which are really scary Q What is your advice to lab managers in a similar situation As First talk to your internal customers and find out how much can be gained by linking your data to those of others in your organization The idea is that your data creates reports and you want those reports to go as far across your organization as they possibly can Also you want to get something that works for you Find a piece of data that is par ticularly onerous and use that in a demonstration to find the weak links in your current software or system We did that and we did end up break ing the software when we evaluated it ACD Labs was very proactive and reactive to what we had found and we worked together through the pilot trial to fix it This did not just involve requests that we were making to suit our workflows and ACD Labs understood that what we were uncovering would make their software a better product May 2014 Lab Manager 37 38 HEALTH amp SAFETY RESPIRATORY PROTECTION BASICS by Vince McLeod n the mid 1980s th
140. ove ambient temperature control e Outdoor placement saves on valuable laboratory space e Simple lift to open mechanism to access samples e Classic design eliminates complicated controls and monitoring e FC free with no compressors and minimal maintenance lever lock prevents pesky raccoons and other animals from contaminating samples 1 888 GROW CF HOW IT WORKS 82 3D CELL CULTURE AND FLUORESCENCE IMAGING Problem Assays using immortalized cell lines and plastic labware have been used for decades as an in vitro mimic of how a putative drug may work in the human body The last decade has seen attempts to improve the physiological relevance of these assays by the incorporation of human primary cells that better reflect human biology both from a drug efficacy and toxicity perspective At issue with the use of primary cells is the relative difficulty in tissue culturing and maintenance for long term studies relative to immortalized cell lines Solution 3D cell culture methods that involve cell aggregation either by self assembly into spheroids or the use of scaffolds to build tissue like structures can enable long term experiments spanning a week or more Peter Banks and Brad Larson of BioTek have shown that human hepatocytes demonstrate excellent plasma membrane integrity and enzyme activity 1 e cytochrome P450 isotype 3A4 over a period of almost two weeks when contained in a spheroid liver microtissue This allow
141. ownloads 91 percent were free Gartner estimates that total downloads will grow to approximately 139 billion in 2014 By many end user accounts apps help improve per sonal efficiency and group coordination and assist in the smoother operation of the entire laboratory enterprise Conveniently designed into compact software packages with highly specialized capabilities apps appear to be a natural fit for mobile smartphones which had greater global sales than their less brainy predecessors for the first time last year and mobile tablets the heir apparent of Lab Manager May 2014 LATEST MOBILE EFFICIENCY SEG ISTRY Capabilities embedded in mobile apps running on a smartphone could store the time the sample was taken and the GPS coordinates of the location FFERINGS DELIVER GREATER HURT AND CO COMPLIANCE the once dominant but bulkier notebook computers Apps running on smartphones or tablet devices have emerged as a convenient yet potent tool to access complex underlying digital systems that required considerable skill time and financial resources to build In operations that many end users may now consider routine apps are downloaded into mobile devices that are capable of functioning in a detached stand alone man ner These devices can be readily connected and synchronized within a lab s network typically to upload test data obtained from instrumentation in the lab or in the field By any measure this is
142. pe For non particulate contaminants APRs should be used only for materials that have good sensory warning prop erties such as odor or irritation This alerts the user to respirator failure either through seal leakage or cartridge overloading OSHA requires use of cartridges canisters with end of service life indicators ESLIs or establishing cartridge change out schedules to reduce the likelihood of contaminant breakthrough These schedules will vary with www labmanager com FLOW SCIENCES INC R A ENGINEERING SAFETY AS N ASK IS HOW FLOWSCIENCES COW APPLICATION a 1 800 849 3429 HEALTH amp SAFETY SAFETY TIP MAKE LEARNING HOW TO BE SAFE AN INTEGRAL PART OF EDUCATION WORK AND PLAY By James A Kaufman For too many years at academic institutions and some companies health safety and the environment have been something extra It s time that they become part of the process At Dow Chemical we were told that we were being paid to do three things 1 work safely 2 conduct active research programs and 3 publish the reports and patent disclosures resulting trom our research Satety was part of the job not something extra The slogan at the Bell System is No job is so important and no service so urgent that we cannot take time to pertorm our work safely At Dow it was each person s responsibility to be sure that their work could be performed safely If you don t think it s safe to
143. ped the two groups of engineers to understand each other s worldviews and to collaborate more effectively Lab Manager May 2014 The fusion approach In each case leadership had unwittingly hit upon a particularly effective approach to managing diverse teams Jeanne Brett director of the Dispute Resolution Center at Northwestern University s Kellogg School of Management and Maddy Janssens of Belgium s Catholic University of Leuven who devised the approach call it fusion They coined the term because of the concept s similarity to fusion cooking which combines ingredi ents or cooking methods from different cultural tradi tions while preserving their distinct flavors textures and forms of presentation In the management context Brett explains Fusion is based on two fundamental elements of collaboration coexistence of differences and meaningful participa tion Those elements ensure that teams reach their goals most effectively In addition Brett says We think we have some evidence that teams with fusion team work systems are more creative Managers of R amp D groups in North America and elsewhere face multicultural situations with increas ing frequency In those situations they must deal with the potential for multiple cultural clashes among team members The fusion approach has the basic goal of al lowing every member to make his or her contribution to achieving the team s goals Fusion teamwork allows dif ferences
144. perature of food items and liquids using an insertion probe e A single press of the REC button allows the user to record not only the temperature but also the time date operator amp item ID s and a pre programmed measurement judgment e Features a measurement range of 25 C to 235 C TandD www tandd com Large Capacity Centrifuge System Ys Sorvall RC BIOS 10 Mb BOOTH 127 e Offers a new 10 liter capacity for batch bioprocessing with the Thermo Scientific Fiberlite F5 10x1000 LEX rotor e This durable non corrosive carbon fiber rotor reduces cycle times by spinning up to 10 1 liter Thermo Scientific Nalgene centrifuge bottles in a single run e Capacity and design make this unit an ideal solution for numerous applications Thermo Fisher Scientific www thermoscientific com Device for Removal of Dying amp Dead Cells ClioCell e Selectively initiates dead cell removal emulating dead cell clearance mechanisms which operate in vivo to keep tissues functioning optimally e Improves viability and quality of cell populations and their subsequent productivity Has been successfully tested on many cell types both human and mammalian e Provides new opportunities to optimize cell growth culture and storage AMSBIO www amsbio com CHEMICALS KITS amp REAGENTS Immunoassay Kits NS GyroMark HT IS BOOTH 40 A N e Designed specifically for use on Gyros Gyrolab xP workstation 3 Mi e Simp
145. ple to participate is to make the size of the group smaller Managers set up their teams to fail because they themselves fail to help the team anticipate cultural differences and so see conflict in cultural as opposed to personal or organizational terms and fail to set norms for dealing with cultural differences such as meaningful partici pation and coexistence the two basic elements of fusion Brett ex plains Managers also fail to come up with integrative creative ways of dealing with the differences in ideas that meaningful participation and fusion generate Instead they revert to dictating the team s solutions or letting a dominant subgroup take over That leads at a minimum to a lost opportunity for the team to manage its own cultural problems Every member contributes The fusion concept aims to overcome that type of problem by ensur ing that every member contributes his or her expertise to the team s discussions That takes careful organization and management One of the ways to get people to participate 1s to make the size of the group smaller Brett explains In one example I studied the group contained 16 people from four different geographical areas They split the task into four parts and assigned a four person multicultural team to each part of the task In another example there were maybe 12 people on the team Lab Manager May 2014 www labmanager com LEADERSHIP amp STAFFING and part of the problem
146. rding with Accelrys Capture directly at the bench For more information go to www accelrys com improves efficiency flexibility and data quality Lab Manager May 2014 www labmanager com PRODUCTS IN ACTION Save Time and Improve Safety in Shared Laboratories Shared laboratory facilities often have a num ber of unique users with varying levels of cen trifuge experience This presents challenges to lab and facility managers responsible for en suring safe operation maximizing uptime and minimizing maintenance costs It s easier to ac complish this with features such as password protection user defined programs and Mobile operation which are built into Beckman Coul ter Life Sciences ultracentrituge and high per formance lines including the new Avanti JXN This gives shared laboratories the reliabil ity and performance of a leading centrifuge brand with the functionality they need for to day s research applications Sample prep made easy Users tind it easy to learn read and navigate the large LCD touchscreen on Optima and Avanti series centrifuges Setting up a manual run to prepare a sample for your research is as simple as inputting speed time and temperature The Windows based user interface makes training and use intuitive while reducing setup time and the possibility for errors The help screen built right into the screen s context makes it simple to navigate A low deck height makes the Optima an
147. red with the use of its clinical ductless hoods SS City 800 821 5525 May 2014 Lab Manager 91 92 PRODUCTS IN ACTION Y PolyScience Temperature Control Solutions www polyscience com Lab Manager Moy 2014 Five Things to Know Before Buying a Liquid Temperature Control Product When buying a new liquid temperature control product there are a number of factors to consider when picking a product Pick the Right Product Make sure you are picking the right product for your application With so much vario tion within each product category determine the differentiating features of the product line and choose the best fit for you For example PolyScience Circulating Baths are available with six different temperature controllers rang ing from basic to sophisticated with standard features ranging from English icon controls to operation in 11 different languages We have options with unlimited programmability op tions and multiple connectivity ports When in doubt contact the distributor or manufacturer s sales team for assistance Think Ahead If you re in the market for a heated circulating bath and have room in the budget consider buying a refrigerated circulating bath if you may be operating at temperatures near or be low ambient in the future It ll save money in the long run as you ll already have the ability to work at those temperatures without purchasing another unit Check f
148. rfna com reference2 800 645 3050 001 A1 0149 B 2014 Eppendorf AG EDITOR S NOTE Breaking Down Silos It is not news that the research workplace has changed considerably over the past decade As with many businesses laboratories have come under pressure to generate better results with ever greater efficiency This in turn has led to greater emphasis on interdisciplinary collaboration which has necessitated the breakdown of previously isolated silos of knowledge and ways of working And whether research entities like it or not cross disciplinary and cross cultural teams aren t going away any time soon The challenge for managers has been how to make these new collaborations productive dynamic and clash free This month we try to provide a bit of guidance Beginning with The Human Side Managing Culturally Diverse Teams author Peter Gwynne introduces a particularly effective approach to managing diverse teams referred to as fusion Fusion is based on two fundamental elements of collaboration coexistence of differences and meaningful participation says Jeanne Brett director of the Dispute Resolution Center at Northwestern University s Kellogg School of Management Managers set up their teams to fail because they themselves fail to help the team anticipate cultural differences and so see contlict in cultural as opposed to personal or organizational terms and fail to set norms for dealing with cultural difference
149. ronmental Chemical Materials 2014 Waters Corporation Waters UPLC and The Science of What s Possible are registered trademarks of Waters Corporation ACQUITY UPC UPC and UltraPerformance Convergence Chromatography are trademarks of Waters Corporation ing work of Apple focusing on particular tasks and ease of use might have helped to reduce generational differences in the use of certain apps In the laboratory we have a similar challenge with our prior technology people were okay with using 1t because of the benefits but the learning curve was relatively high Now though you can see someone picking up our Capture app and even though they may not be comfortable with an onscreen keyboard they can handwrite into the app and their handwriting is automatically con verted into digital text for storage in the database He adds Features like those make the adoption easier and fun there is a coolness factor and the time it takes to learn and use the app is almost zero We stress functionality but usability is also a very high priority for us Tetreault says The vision for the future 1s to continue to build focused applications to aid laboratory operations so that all aspects of lab activities are done electronically through apps for managing inventory calibrating equipment collecting data providing dashboards and managing resourc es among other functions User friendly apps with a growing range of fun
150. rp helps when you have a weak emitter or fluorescent proteins with a small Stokes shift www labmanager com CY TATION gt imaging reader Powerful Automated Affordable Bench top cell imaging and analysis for a wide range of applications www cytation3 com Think Possible VO Bi a j Y L8 N h www biotek com PRODUCT FOCUS MICROPLATE READERS Beyond adjusting emission and detection of fluorescence some users will want a microplate reader that provides as many options as possible For example a microplate reader in a core lab might be applied to a range of experiments In such a situation a reader that works in various ways could be the best choice For example some microplate readers work in fluorescence and luminescence modes plus absorbance in some models As Earp points out moving from one mode to the other can be easy In ours he says you just click a button in the software Then it s all handled automatically A core lab might also seek that level of ease of use to accommodate users of various levels of experience As well volumes decrease and cell based assays diversify the features of microplate readers keep increasing Shrinking the size For people looking at DNA RNA or protein quantification says Michael Fejtl market manager of detection at Tecan Austria in Groedig they like small volumes like two microliter volumes He adds
151. rs offered in terms of performance and ergonomics Is the product compatible with other manufacturers consumables tips What types of services are offered for this product Calibration Repair e Does the company offer application support and technical phone support before and after product purchase e What is the product life expectation What is the product s warranty period If the company discontinues the product for how many years do they provide accessories and parts for the instrument TOP 10 FEATURES FACTORS respondents look for when purchasing a pipette CONSISTENT PERFORMANCE AND ACCURACY OF PRODUCT 90 E gt lt DURABILITY OF PRODUCT 81 O kk AVAILABILITY OF TIPS AND ACCESSORIES 75 RO LOW MAINTENANCE EASY TO CLEAN 63 O DESIGNED TO PERMIT RECALIBRATION 58 O lt lt lt PRICE 58 A ME SERVICE AND SUPPORT 49 A acne VVARRANTIES 48 _ AR ERGONOMIC OPERATION 47 at Completed Surveys 907 5 For more information on pipettes including useful articles and a list of manufacturers visit www labmanager com pipettes 78 Lab Manager Moy 2014 www labmanager com The WaterPro BT delivers ultrapure water manually or volumetrically directly from tap water This system is ideal for laboratories needing between 1 and 10L of RO and or ultrapure Type water per day Small footprint so it can fit in tight spaces Only 16 5
152. rs only have fixed or limited band widths up to 30 nm mTFP1 YFP 1 MIEpi YFP Tandem FRET a E 0 3 sos MFP YIP blue yellow E control 4 0 6 mTFP1 YFP tandem 0 4 E Ot 2 E aso 500 550 600 650 Wavelength nmi Ratio D A mTFP1 TYP mTFPI YFP Change control tandem FRET Confocal Microscope 0 89 0 54 37 1 480 30 535 45 nm CLARIOstar 37 9 485 30 525 25 nm New LVF Monochromators Have Filter like Performance Realizing the need for a more sensitive broader bandwidth monochromator in a microplate read er BMG LABTECH s German engineers created the CLARIOstar multimode microplate reader with new innovative LVF Monochromators Consisting of linear variable filters separated by a linear variable dichroic mirror LVF Monochro mators filter light into definable wavelengths and bandwidths up to 100 nm wide Confocal Microscope to Microplate Reader No Filters Needed Researchers routinely try to adapt their fluo rescent protein assay from a confocal micro scope to a microplate format Now with LVF Monochromators no filters are needed The figure shows an mTFP1 YFP fluorescent protein FRET response in HEK293 cells as CLAM Ostar with Monochromators measured on a confocal microscope and on the CLARIOstar This FRET assay requires the measurement of two emission signals with bandwidths of 30 nm and 45 nm for the mi croscope 2 which were the starti
153. s Brett explains Turn to page 26 to learn more nu As for cross disciplinary collaboration Mark Lanfear in this month s Science Matters column page 16 says Merely bringing everyone together on a project isn t necessarily going to do the trick Meaning ful cross functional connections must be made In addition to good communication and making sure that everyone knows they are no longer working separately managers need to start thinking in a more interdisciplinary fashion This involves knowing that different perspectives on a single project are the key to inspiring new ideas which Lanfear believes will make the work being done more efficient dynamic and ultimately of higher quality If the existing organizational structure of a lab could be considered a kind of silo then this month s Lab Manager Academy author Steve Epner suggests breaking that down as well Most lab operations need to understand the difference between leaders managers and doers This lack of understanding is the reason so many labs are structured around people based on their longevity and personalities instead of their performance He argues that successful organizations start with a clean sheet of paper No names just an understanding of where you need leaders managers and doers Turn to page 18 for more Whether your management challenges are cross cultural cross disciplinary cross generational or structural you are s
154. s Calibration curves with unlimited number of standards Linear Quadratic and Cubic calibration curves Chromatogram overlays Bracketing of samples Digital control of a range of peripherals Call us today for a free consultation E 800 562 566 J BUC 203 853 9444 www bucksci com Sales bucksci com Scientific 42 PRODUCT FOCUS LABORATORY GAS GENERATORS GENERATORS LABORATORY GAS Lab Manager RELIABILITY CONTROL KEY ISSUES by Angelo DePalma PhD or David Hemmig director of F equipment sales at Matheson Tri Gas Basking Ridge NJ the key word in on site gas generation is reliability He explains that a major problem with gas generators that incorporate some sort of compressor is that eventually that component fails Nitrogen and zero air generators for example often use compressors to bring air into the system The zero air generator fine tunes the product by removing hydrocarbons But the compressor is the weak link Hemmig says For nitrogen generators the compressor serves a similar purpose but separating pure nitrogen from air which is 78 percent nitrogen is slightly more difficult Membranes represent the established technology while units from Air Products Allentown PA for example use pressure swing adsorption Regardless when the compressor fails nitrogen generation ceases H Hydfogen 1 00794 May 2014 Failure rate is why we avoid compr
155. s control module regulates CO2 and O2 con centrations for optimal physiological conditions and pH buffering Adding these environmental variables directly to the reading and imag ing chamber reduces cell culture exposure to unregulated lab atmospheres and fluctuating temperatures that may adversely impact results The combination of multi detection reading and microscopy along with integrated cell in cubation allows for endpoint time lapse and montage information to simplity research and assay development and increase throughput in cell biology research www labmanager com PRODUCTS IN ACTION Microscope to Microplate Reader Increasing the Throughput of Live Fluorescent Protein FRET Assays Introduction Filters vs Monochromators When using a microplate reader these are the two options to filter light into monochromatic wavelengths Filters offered higher performance because of greater light transmission and wider band widths monochromators offered greater flex ibility no new filters were needed for each new assay However researchers state that monochroma tors cannot perform many assays 1 Fluo rescent protein assays like GFP mCherry or CFP YFP do not perform well on monochro mator based microplate readers The same holds true for FRET and BRET assays One main reason is that wider bandwidths are needed Some FRET or BRET assays require an emission bandpass up to 100 nm and current mono chromato
156. s for drug toxicity testing over the same period The spheroids consist of aggregations of about 1 000 2 000 cells and have a diameter of about 200 400 um Larger size spheroids tend to necrosis due to the lack of nutrients accessing the core Most assays involving spheroids are conducted in microplates where there is one spheroid microplate well Spheroids are small in relation to the well dimensions and only cover about 0 2 percent of the surface area at the bottom of a 96 well microplate Using conventional PMT based plate reader optics assays utilizing spheroids tend to be problematic as these instruments are designed to capture as much light from the well as possible This causes issues with background as the signal is confined to such a small part of the detection volume Fluorescence microscopy however limits the field of view depending on the magnification desired and thus enhances signal to background ratios S B allowing for robust assays Furthermore image analysis provides additional data for a better understanding of what is occurring in an experiment Figure 1 illustrates this point using the affect of the non steroidal anti inflammatory drug diclofenac on liver microtissue spheroids after ten days repeated treatment under various doses 0 300 uM Images Figure 1A and image analysis Figure 1B D were provided by BioTek Instruments Cytation 3 cell imaging microplate reader using its fluorescence microscopy module under
157. s immediately to temperature They want to see how far they can push it high and low and still get reliable data he says For now the desirable range starts higher than 100 degrees Celsius and goes down below 20 with some people looking to drop that even more In addition says Swertfeger People want to take traditional methods and make them faster At Core Laboratories Canada in Calgary Ken Chong general manager and vice president uses a viscometer for hydrocarbon samples of from light to very heavy oils When asked about the most important features of a viscometer he says Density and viscosity over a range of temperatures on a single charge and the minimal volume it requires to accomplish this He adds The former makes it very efficient and the latter is very important for heavy oil where sample volumes can be limiting The features that matter most though depend fundamentally on the materials being tested So setting up the best viscosity measuring system will vary from lab to lab Reasons to be unreliable With older technology says Swertfeger sample handling and maintenance are huge factors in getting repeatable results He adds that newer more automated devices usually just need to be kept clean If you make measurements in a dirty environment you tend to have more problems he says For any viscometer though he recommends regular maintenance He says For anyth
158. s to maximize light delivery to the sample and detector and dedicated filter optics are optimized for live cell assays Monochromator optics use quadruple diffraction gratings to concentrate and purity the selected wavelength thus optimizing spectral discrimination User selectable monochromator optics also allow for wavelength scanning and kinetic measurements Multiple parallel detectors decrease measuring time and both optical systems may be read trom the top or bottom of a microplate for increased assay versatility CELL MICROSCOPY Cytation3 automates cell microscopy throughput compared to manual fluorescence microscopy and also allows simple assay validation before moving to high content screening An inverted fluorescence microscope with brighttield capa bility and autofocus is integrated in Cytation3 or available as an upgradeable option Fluo rescence microscopy and color switching are available through red Texas red green GFP and blue DAPI LED filter cubes and brighttield images are taken with a simple white light Additionally 2 5x and 4x objectives allow researchers to view and read entire microplate wells while 10x and 20x objectives allow viewing and reading of intracellular details CELL PROPAGATION Cytation3 offers uniform temperature control up to 45 C across the culture chamber and vari able orbital shaking to keep cells in suspension even during long experiments An optional ga
159. st also ensure that the respirator fits the wearer A respirator that does not fit properly simply gives the false impression of protec tion and may be more dangerous than no respirator at all There are specific fit test protocols that are accepted by OSHA The following is an excerpt from an OSHA compliance directive 1 Respirators must be fit tested 2 A test atmosphere must be applied to assess the quality of fit 3 The fit test must be applied to each and every employee required to wear a respirator 4 The fit testing requirement applies to all negative pressure respirators including SINGLE USE RESPIRATORS Respirators allow us to go where we could not normally enter safely The use of respirators should not be taken lightly or in a cavalier man ner When used improperly they can put people in greater jeopardy Remember respirators should be used only as a last line of defense when other control systems such as adequate ventilation or hazard containment are not feasible References 1 Guidelines for Law Enforcement for the Cleanup of Clandestine Drug Laboratories US Department of Justice Drug Enforcement Agency Washington D C http www yustice gov dea resources img redbook pdf 2 Respiratory Protection US Department of Labor Occupational Safety and Health Administration Washington D C https www osha gov pls oshaweb owadisp show_document p_table standards amp p_id 12716 Vince McLeod is an in
160. supervisor and lab manager He has been with Agropur for seven years and says that the role of the lab has changed in that time as customer quality and safety requirements have led to increased Better Detectors produce Better Results PHOTONIS is the 1 detector supplier to most analytical instrument manufacturers Our high quality detectors provide longer sustained output and greater longevity for more durable and reliable results Switch to PHOTONIS for a wide range of high quality custom and standard detectors Microchamnel Plates Superior Gain and Resolution with Low Noise Time of Flight Detectors Reduced Time Jitter to Improve Mass Resolution Advanced Performance Detectors Complete Assemblies with Quality MCPs Resistive Glass lon Transport Tubes Up to 1000X Increase in lon Throughput Channeltron Electron Multipliers Extended Dynamic Range at High Pressure Ask about our complete line of replacement detectors S PHOTONIS ee For more information visit www photonis com or email sales usa photonis com 2 Ferry LaBelle working with protein analyzer responsibilities As customers make more demands for food safety programs we look at the resources of the plant and work to meet the new requirements Labelle explains The increase in responsibility has led to hiring more staff There has also been an increase in data entry into Oracle and various spreadsheets with two to three hours spent on
161. t hits you All of this data could more easily be collected not to mention transmitted back to the lab on a single device for example the Phone in your pocket Combining mobile devices and a LIMS brings a sampling program s entire chain of custody under one secure umbrella Mobile devices are an increasingly critical component of modern life and that trend holds true for laboratories as well Regardless of industry the incredible and constantly evolving features on these devices can enable technicians to easily capture new types of data more accurately and from more remote locations but they also pose an interesting challenge How do laboratories ensure that all the data collected via mobile devices is accurate secure and organized The answer is a Laboratory Information Man agement System LIMS Just as a LIMS enables an automated paperless environment inside the lab it can integrate with mobile devices in the field to ensure that data collection transmission and analysis are fully optimized New Devices New Opportunities The most straightforward benefit mobile devices bring to the lab 1s quite simply more data The more data that labs have available the more effective they can be provided that the data is organized and accurate That s where the LIMS comes in A LIMS enables users to transmit information directly from the field into the database eliminating error prone manual transcrip
162. t there are still many misunderstandings and miscon ceptions surrounding the use of respiratory protection whether it is in response to a possible chemical spill incident in the lab processing chemical wastes in confined spaces or just dealing with routine chemicals in your normal activities Vince McLeod FAKE AD More info on page 96 May 2014 ger STAYING SAFE A recent incident at UCLA high lights just how important it is to be vigilant about safety in the lab In that accident a student was burned on his face and neck after a lab oven exploded during an experiment in April Though the student was lucky his injuries were not life threatening proper safety procedures likely could have prevented the incident from happening altogether Could this have happened in your lab What about your colleagues Next month we will find out how well our readers enforce safety proce dures in their labs as we release the results of our Fifth Annual Laboratory Safety Survey Last year s survey showed a significant backsliding in lab health and safety practices Here s hoping for better results this year www labmanager com Take control of lab workflow NEW An all inclusive informatics solution that gives analytical and QA QC labs complete control over methods and SOPs without having to purchase integrate and validate software from multiole vendors The new Thermo Scientific Lab Execution System LES is web based
163. testing and a lot of finished product testing However the lab is involved in much more From the farm to the table The entire process starts at the 150 farms that the facility receives its milk shipments from In the dairy industry it 1s a requirement that every load of milk must be tested for antibiotics before unloading and if antibiotics are found the entire shipment 1s rejected Lab Manager May 2014 PERSPECTIVE ON A FOOD AND BEVERAGE LAB h 2 Lab supervisor Ferry LaBelle A BARTAKONIO l P P Dy y gt 4 ne q E As a result the laboratory is involved i with the farmers long before the milk arrives in tankers at the facility The laboratory accepts samples from farmers that the lab sends away for antibiotic testing to ensure that the cows milk has no traces of antibiotics in it If a farmer had a sick cow that was being treated with antibiotics the testing would give farmers the information they need to be confident the milk they send to the facility would be accepted The quality of milk is also important to Agropur and farmers are able to have samples sent away for somatic cell testing through the lab When the milk arrives it 1s first tested for antibiotics by an intake receiver who 1s trained to use the Charm three minute test If the milk tests positive for antibiotics the sample is sent to the lab and tested a second time The lab at the Weyauwega facility is certifi
164. the most If possible modify your LIMS to capture this data so it can be more readily accessed If a large percentage of your samples come from pri vate citizens or local small businesses consider adding similarly worded questions to your COC Where did you collect your sample house apartment or business What made you decide to test your water today bought a new home selling a home someone in the household is pregnant or you have an infant living in the home annual testing to check the safety of your drinking water it tastes funny your water has an odor the water isn t clear or has a color or solids present or discolor ation noticed in sinks toilets or laundry Lastly ask your clients how they heard about your lab s services Did they find you on the Internet Did the newspaper or other media mention your lab or services in a story Lab Manager May 2014 Did their doctor refer them or was it a neighbor or a friend Did a business like a realtor or well driller recommend they get their water tested Why Private Citizens Test Their Drinking Water 0 2 4 Annual Regular Testing Occasional Testing E Check Drinking Water Safety E Concerned About Taste or Appearance of water E New Well Repairs E New Home Selling E Doctor Dentist Recommended E Pregnancy Infant E Medical or Health Concerns Other 29 3 2 Source Public Health Madison amp Dane County PHMDC Laboratory If your clien
165. the impact of mobile technologies 1s really felt in the execution phase of laboratory projects This is where the researchers are up from their desk they are moving around and picking up their assignments samples and materials He notes that in the lab today lab staff will likely have tablet devices on which they will see a listing of the work to be done Right on the smart device they can open up the work order and follow the associated procedures preparation of a sample or equipment or execution of an analytical test all of which are considered lab work According to Tetreault Traditionally that lab work was done on paper and in some cases on our first generation laboratory execution system LES but this represents the greatest role for our mobile application systems The pioneering work of Apple focus ing on particular tasks and ease of use might nave helped to reduce generational differences in the use of certain apps Accelrys Capture is used to collect information on experiments in progress Tetreault continues An experi ment might involve preparing a solution collecting the pH and mixing the solution with a variety of different liquids To execute this technicians will open the Cap ture app which will allow them to take a picture of the solution add in notes on the procedure they are following and input voice recordings among other steps Capture allows all these inputs at the same time as th
166. till in the business of increasing revenue for your lab and for that you need a marketing plan Turn to this month s Business Management article Marketing Your Lab to learn some easy inexpensive and practical suggestions for doing just that Marketing your lab can be ac complished with a little elbow grease and some legwork often at little cost By doing research up front on potential customers and planning your marketing based on the targeted audience you will be more successful reaching your customers with the right message says author Lynda Seeger This month s issue also provides the latest information on laboratory imaging systems INSIGHTS page 62 the challenges of managing a dairy lab Perspective On page 56 and productspecific articles on gas generators microplate readers mass spectrometers viscometers and LIMS all beginning on page 42 All in all some good information we hope you find useful Best Pamela Ahlberg Editor in Chief Lab Manager May 2014 906 a E gt C a Moisture Analysis Ww Moisture Analysis app support O Moisture Analysis tech support a Moisture Analysis sales support Q Moisture Analysis on site service y q The Search is Over Extraordinary Product Extraordinary Team METTLER TOLEDO Excellence moisture analyzer solutions make selection easy Our innovation features the brawn of our industry leading halog
167. tivity in dealing with cultural differences e Better understanding between team members of the others worldview DOCUMENT OR DIE INSIGHTS ON IMAGING SYSTEMS Keeping track of everything is especially critical in the cell culture Imaging encompasses a wide range of techniques that enable visual field in order to ensure that the data from cell culture research is ization of hidden features of samples structures or organisms The accurate and to prevent incorrect information from being published latest developments in imaging include Proper documentation also e Increased ease of use e Allows labs to trace problems back to their source e Unattended imaging of multiple samples enabled by automation e s necessary for some labs to meet industry regulations e Confocal microscopy and multiphoton imaging now routine e Ensures consistency reproducibility and product quality e Mass spectrometry pushed to the forefront of advanced imaging technologies e s crucial in maintaining a record of experimental procedures during R amp D 98 Lab Manager www labmanager com Agilent Technologies Inc 2014 OF PARTS SUPPLIES OF INSTRUMENTS SERVICED NAME TO CALL Agilent CrossLab delivers quality parts and supplies tested to ensure seamless performance with major brands of GC and HPLC instruments Our expert service engineers are certified to repair maintain and ensure regulatory compliance of instrument platforms from all ma
168. to mitigate due to the budgeHocused nature of many hospital and clinical laboratories So how do we go about protecting our technicians trom the chemi cals they have to use on a daily basis and do so without breaking the bank Labconco has two options the Fume Adsorber and the Protector Work Station For those troublesome stains and nuisance odors the Fume Adsorber offers a compact foot print only 30 wide and 15 4 tall and flexible ductless filtration on a reasonable budget The Fume Adsorber is available with Organic Vapor for xylene toluene organic stains or their substitutes formaldehyde or ammonia carbon based filters When analyzing or preparing tissues for storage technicians use more than minute quanti A ties of chemicals For these procedures something more robust is required Enter the Protec By Brian Garrett tor Work Station It is the ideal enclosure for clinical pathologists and histologists lt also has LEED Green Associate Product Manager a small footprint 29 deep x 43 wide but uses stackable carbon filters for larger chemical capacity or mixing of chemical uses pair two identical filters or two different filter types When small scale protection is needed going to a large fume hood isn t always a suitable Protecting your lt gt laboratory environment gt Z LABCONCO Labconco Corporation Kansas option Labconco has the protection of these smaller operations cove
169. togenetics simultaneous electrophysiology and photoactivation techniques such as photostimulation and photablation All these involve selective targeted interaction between light and matter usually with cells resulting in per turbations detected by image capture and rendering elements Jeff Stuckey PhD product marketing manager for fluorescence microscopy at Bruker Nano formerly of Prairie Technologies explains that photoacti vation does not actually move sample constituents in space For that you need laser tweezers which we don t do Instead we activate molecules using laser light In caging experiments for example lasers cause the release of neurotransmitters from nerve cells In another form of photoactivation optogenetics neurons are stimulated to mimic naturally occurring neuronal activity which allows investigators to image neuronal connections and activity Because fluorescence occurs only where photons arrive simultaneously fluorescence 1s limited to tiny well defined regions Stuckey explains The analogous confocal imaging technique results 1n diffuse fluores cence above and below the desired location resulting in photobleaching which contributes to out of focus light Another advantage of two photon fluorescence excitation involves its use of infrared lasers which penetrate more deeply than visible light into tissue Where visible wave lengths image to a depth of about 300 microns two ph
170. ts are more businesses and municipalities consider them a good source of information Ask them how they heard about your services Find out if they know others who need the same testing Ask why they do the test ing s it required grant funded or for safety concerns By asking some of these types of questions you will learn how clients became aware of your lab services and why they are performing the testing Once you have this information you can more effectively focus your marketing to reach new clients that may have the same needs or concerns A strong marketing plan will consist of a multifaceted approach First an easy to use website is essential Have someone outside your agency act as a potential client www labmanager com y DIVStmar a Trust it impeccably Use it comfortably Achieve accuracy and reproducibility with the ergonomic PIPETMAN L designed with your results in mind Lockable volume ensures accuracy when aspirating and dispensing Ergonomic tip ejection button for both right and left handed users Easy to use lightweight design reduces hand fatigue during repeated pipetting Barcoding with traceable serial number for enhanced GLP compliance Available in single multichannel and fixed volume models mr GILSON instruments accessories software solutions gt www gilson com Perfect limes Make BINE Your Ovens ar Incubators Destinatio gt Oven temperatures
171. tuitive user interface For example the new Accelrys Capture mobile data recording app for laboratory informatics offers one of the simplest most intuitive mobile ELN interfaces on the market today Accelrys Capture 1s as easy to use as paper while providing the additional advantages of an integrated electronic solution Scientists get a quick overview of y their tasks and experiments They can look up current procedures and enter data using a single device right at the lab bench where the work is performed Eliminating data transcription improves data quality Additionally scientists can take advantage of advanced usability features commonly found on mobile devices such as voice recognition camera swipe UI etc Accelrys Capture is directly connected to the Accelrys Enterprise Platform enabling instantaneous data transfer high data integrity security and powerful search capabilities With apps such as Accelrys Capture scientists can eliminate non value added workflow steps to work faster more flexibly and intuitively in the lab ultimately focusing more of their attention on the science at hand Lab managers will see better quality improved data security and integrity fewer workflow bottlenecks and enhanced staff motivation Overall scientists equipped with such an app will see improved productivity and faster experimentation with shorter cycle times enabling them to make better informed decisions A Data reco
172. tup offers rigorous control of the temperature and CO2 and 2 partial pressures within the measurement chamber allowing in reader incubation and complete automation of the assay Methods Target cells Raji cells and WIL2 S cells were thawed and mixed with assay buffer RPMI 1640 Figure 1 Tecans Infinite 200 PRO medium 4 low lgG serum then plated into the inner 60 wells of two 96 well plates perimeter multimode microplate reader and wells filled with assay buffer A dilution series of control antibody Anti CD20 was added to both Gas Control Module GCM plates in final concentrations trom 6 pg ml to 0 73 ng ml for Raji cells and 4 ug ml to 0 14 ng ml tor WIL2S cells Wells without antibody served as negative controls and wells containing only buffer served as reagent background controls Effector cells were prepared in the same manner as the target cells and added to the inner 60 wells of both plates then briefly mixed on an orbital shaker The plates were then incubated in the Infinite 200 PRO or a tissue culture incubator at 37 C 5 CO2 for six hours Following incubation BioGlo reagent Bio Glo Luciferase Assay Substrate in Bio Glo Luciferase Assay Buffer was added to the experimental and background control wells and incubated within the Infinite 200 PRO measurement chamber at 37 C for a further five minutes prior to luminescence measurements A B ED Raji STC 50 Wi2 5 STC de Ra
173. umerous tloors and rooms with a constant pure stream of hydrogen gas at the flick of a switch With a production rate of 4 8 9 6 or 18 8 slom Proton OnSite s compact S Series hydrogen gen erator produces the equivalent of four cylinders of betterthan ultra high purity grade hydrogen every day Proton OnSite hydrogen generation systems help many industries eliminate the cost associated with delivering and using hydrogen e PROTON NA e THE LEADER IN ON SITE GAS GENERATION Proton OnSite 10 Technology Drive Wallingford CT 06492 01 203 949 869 www protononsite com May 2014 Lab Manager 93 94 APPLICATION NOTE Performing Promega s ADCC Reporter Bioassay with Tecan Infinite 200 PRO and GCM Introduction Antibody dependent cell mediated cytotoxicity ADCC is an important target for antibody derived biopharmaceuticals allowing the controlled targeting and destruction of tumor virus intected or other diseased cells Promega s ADCC Reporter Bioassay offers a convenient way of assessing ADCC us ing frozen thaw and use effector and target cells eliminating the need for cell culture maintenance This assay requires a six hour incubation of cells with the antibody of interest for optimal performance typically in a standard cell culture incubator This paper evaluates an alternative method of performing the assay using Tecan s Infinite 200 PRO and Gas Control Module GCM figure 1 This multimode reader se
174. use a respirator unless he or she is first given a medical evaluation and then tested to ensure that the respirator provided will actually fit to provide protection In addition anywhere respirators are used to protect one s health against respiratory hazards OSHA requires development of a respira tory protection program in accordance with 29 CFR 1910 134 Where respirators are used on a voluntary PUMP UP YOUR LAB INSTRUMENTS with GHD Rotary Vane Pumps from ULVAC Call 800 99ULVAC or email sales us ulvac com for the pump ULVAC com that has it all clean quiet affordable cool and compact GHD HEALTH amp SAFETY basis fewer program elements are required to prevent hazards associated with respirator misuse Suffice it to say that if your organization is using respirators you should have a written program and an administrator well versed in this OSHA standard Before a user ever puts on a respirator OSHA requires and it makes good sense assurance that users are physically capable of wearing a respirator OSHA 29 CFR 1910 134 states The employer shall provide a medical evaluation to determine the employee s ability to use a respi rator before the employee is fit tested or required to use the respirator in the workplace It goes on to say Employees that use any respirators including respirators such as air supplied positive pressure face masks or hoods must have a medical evaluation before use One mu
175. using the blue fluorescence channel dead cells represents the percentage of the total cell count containing green fluorescence www labmanager com Strengthen your pipette quality program Gain confidence by improving quality oroductivity and compliance with Artel technology and certification training Improve knowledge amp skill Manage performance amp inventory Hands on expert and scientifically rigorous coaching Fast and easy pipette calibration with instant feedback encompassing proper technique care calibration ideal for teaching proper pipetting technique maintenance and regulatory compliance Proven to Robust documentation and pipette inventory improve pipetting accuracy and precision management features ensure the highest standards of pipetting quality assurance 3 ARTEL Trust Your Results www artel usa com CORPORATE HEADQUARTERS EUROPEAN OFFICE 25 Bradley Drive Westbrook ME 04092 2013 Im Grossacker 46 79252 Stegen Germany Follow Us D fin T 888 406 3463 207 854 0860 T 49 0 7661 982064 F 207 854 0867 F 49 0 7661 982065 E info artel usa com E sschork artel usa com 2014 Artel Inc HOW IT WORKS 84 INCREASED LAB EFFICIENCY WITH MOBILE DATA CAPTURE Problem Scientists who are still using paper in the lab might be doing so because they consider it the easiest way to work But paper doesn t allow the benefits of electronic solutions like automated data transfer and sh
176. val easier A major issue Merli says is users assumption that the next user will clean the centrifuge You end up discovering that the centrifuge has never been checked or maintained for months and months Merli says Simply cleaning out the rotor chamber is important in keeping centrifuges in top form Some of Thermo Scientific s models have a feature that allows users to remove the rotor with the push of a button lt gt Labnela LEA E t Erra LL E Inc que labnetinterational com labnetinfo corning com MAINTENANCE MATTERS One way users are solving this issue is by hav ing a chart next to the centrifuge similar to those found in public bathrooms showing when it was last cleaned and who cleaned it Another key to a happy unit is inspecting compo nents such as O rings Our standard practice is every time you re going to use a centrifuge especially floor model or ultracentri fuges is to inspect all of the critical components look at the O rings and gaskets Lockner says Look at the hub for any signs of wear or unusual marks Any strange noises or vibrations might warrant a service call Lockner says that many of the mistakes people once made with centrifuge maintenance have been eliminated with the features of the latest models but most of the remaining centrifuge problems are caused by imbalance It s important that users are trained on the proper use of the systems Lo
177. versed phase columns such as C18 yielding similar retention characteristics to reversed phase LC when analyzing highly lipophilic compounds The ability to span this wide range of selectivity is what makes convergence chromatography such a powerful tool Convergence chromatography and the ACQUITY UPC2 System The ACQUITY UPC2 System is a holistically designed convergence chromatography system that utilizes liquid CO2 as the primary mobile phase and is the first analytical system pur posetully designed to manage supercritical flu ids The system leverages the chromatographic principles and selectivity space of normal phase LC while providing the ease of use of reversed phase LC enabling you to address routine and complex separations challenges Built upon proven low dispersion high efficiency UPLC technology the ACQUITY UPC2 System offers a level of reliability robustness sensitivity and throughput never before possible The advancements made in the ACQUITY UPC2 System over and above traditional analytical SFC finally enable normalphase separations to be done robustly and reproducibly so that con vergence chromatography can be as routinely applicable as any other separation technique Waters THE SCIENCE OF WHAT S POSSIBLE Waters Corporation 34 Maple Street Milford MA 01757 USA Tell free 800 252 4752 Tel 508 478 2000 May 2014 Lab Manager 95 PROFILES MARKETPLACE 96 ESS INTRODUCES CAPSU
178. ves through production ensuring that it meets customer specifications As customers make more demands for food safety programs we look at the resources of the plant and work to meet the new requirements Throughout the day samples are prepared for shipping out the following day and any antibiotic test results that come from the external lab are relayed to the farmers by the technicians When the night shift comes in they continue the work done in the microbiology lab All the technicians maintain records of analysis and enter the data into Oracle which allows them to compile information about the product and gives Labelle all the information he needs before he approves the product for shipping All documentation and analysis is sent to the state of Wisconsin which has a split sampling program in place with the facility and that audits the lab to ensure that the lab meets the requirements to be certified In addition to monitoring production the lab monitors air quality in the plant as well as monitoring central sanitizing systems and foot foamers and performs environmental swabbing t Year Warranty with GenTech installation Quick Delivery Great Prices me E ew ae for Agilent Thermo Waters amp AB Sciex LC MS Systems for Gas Chromatography Spectro UV Vis NEW bit NEW DART TECHNOLOGY Dual or Single Beam E ID CUBE Variable Bandwidth Available I source SPECTROPHOTOMETERS MLS
179. visit www rigakuraman com Lab Manager May 2014 GC Liquid Autosampler eS TriPlus 100 LS MES BOOTH 127 e Performs liquid injections in a completely automated and reliable manner regardless of the matrix or the selected technique With the addition of optional accessories the system is capable of automating sample preparation steps such as standard dilution standard and internal standard addition and vortexing Can handle almost any type of liquid samples Thermo Fisher Scientific www thermoscientific com Macroion Mobility Spectrometer MacrolMS Model 3982 eme e A powerful new tool for rapid high resolution E molecular mass and size analysis of macromolecules e Offers proven utility in a variety of biochemical applications including analysis of antibody aggregation analysis lipoproteins i viruses vaccines VLPs polymers and nanoparticle colloids rage e Boasts a number of new features over previous models including its ability to perform automated analyses by means of direct connection to LC pumps and auto samplers TSI www tsi com Mass Spectrometry Integration Solution MS ionKey MS System Mi BOOTH 113 e Anew approach for getting exceptional sensitivity robustness and ease of use out of mass spectrometry e Integrates UPLC separation into the source of the mass spectrometer Provides a simplified user experience and the power to perform multiple analyses on limited sample volumes Gives users the ability
180. was that the lower level people from one cul ture would not contribute when higher level team members from their culture and area of the company were present Here the team was split into a couple of lower level working groups with mixed cultures and these teams reported up to the higher level members It took the Ameri cans a while to accept this solution as they were less status oriented but they found that this structure released a lot of knowledge from the status sensitive lower level counterparts and so they went along Brett offers another way to ensure full participation in team discus sions seeding the group with someone who 1s likely to support the team member who has not been participating A seeded member of this type who has a comprehensive understanding of the strengths and knowl edge that each other member brings to the team can give the nonpar ticipant opportunities to contribute his or her expertise to discussions To deal with disagreements in a neutral way Brett and Janssens sug gest team leaders should put the issues to a vote And if one cultural group consistently wins the votes and threatens to overpower other team members Brett recommends that team leader should intervene for example by encouraging more questioning among team members or appointing individuals or again setting up small groups to work on particular problems and share their solutions with the entire team To take maximum advantage of individuals
181. wer of NuGenesis 8 for yourself visit waters com nugenesis8 Waters THE SCIENCE OF WHAT S POSSIBLE Pharmaceutical Health Sciences Food amp Environmental Chemical Materials 2014 Waters Corporation Waters NuGenesis and The Science of What s Possible are registered trademarks of Waters Corporation 20 BUSINESS MANAGEMENT ANALYSIS ELBOW GREASE AND LEGWORK CAN BRING IN NEW BUSINESS AT LITTLE OR NO COST s budgets for agencies tighten available grant money shrinks and money for lab testing becomes less of a priority 1t 1s increasingly important for labs to draw in new business to increase revenue While there is no quick fix labs must figure out who their potential clients are before trying to market their services By identifying these clients who they are where they can be found and what they need labs can specifically target their marketing to these clients To determine who the clients you are trying to reach are first look to your current client list Are they new home owners pregnant mothers small businesses water utilities etc Are they located locally in your state or all over the country What kind of testing are they re questing If you are unable to easily answer these types of questions consider revamping your chain of custody COC forms by adding a few questions with check boxes This way when future samples are brought in you can tally results to see who uses your lab services
182. when almost everyone has a smartphone says Barker The ques tion becomes do you want to buy a stand alone meter or do you want to use a lot of the features that are in the smartphones including their fantastic displays In any case to develop a stand alone device that comes even close to the technology in the smartphone will incur huge costs Barker says What is interesting about these apps 1s that they have expanded our end user sales We are now going into environmental monitoring applications any industrial processes that need product quality control in portable units homes commercial settings pool and spa testing aquaculture horticulture and hydropon ics which is a huge industry now mu nicipal water sampling wastewater for cli ents municipal testing school and college labs and industrial labs among others Compliance testing is another area in which we have a lot of requests Smart Order Now 305 397 3824 megalabtechnology gmail com www megalabtechnology com www labmanager com GOOD CHEMISTRY phones actually save the data so there is no need to write it on paper By doing that the numbers can be validated versus someone using a test strip writing what they think the result is or even fudging the numbers This approach actually gives the person a device that stores the data and saves it without allowing anyone to make internal adjustments Barker says that there 1s
183. y e Offers a clever bench top design and innovative high end technology e Provides a high degree of precision method flexibility and operating comfort e Technological advances in the product include the optical resolution the plasma torch design and the generation and observation of the plasma Analytik Jena www analytik jena com Micro Gas Chromatograph CALIDUS e Provides users with the durability utility reliability and economy it takes to be applied in lab online at line and in the field with universal success Sturdy 1 8 gauge aluminum housing makes the unit tough enough for demanding applications Features a light weight of only 25 lbs and small footprint of just over 1 sq ft Cycles 10 to 50 times faster than traditional GCs Falcon Analytical www falconfast net ICP OES Spectrometer ULTIMA Expert e Combines ease of use a unique plasma torch design comprehensive tools high resolution low detection limits and full wavelength coverage optics for the most challenging applications e Designed with a unique plasma torch that offers a radial viewing mode e More tolerant to difficult matrices and offers the h benefits of robust operation with minimal maintenance HORIBA www horiba com scientific Lab Manager Moy 2014 This month we highlight companies who will be exhibiting at the 62nd ASMS Conference on Mass Spectrometry and Allied Topics ASMS 2014 which will take place June 15 19 2014 at the Baltimore
184. y not make up a majority of the team directs the team s collection of information and decision making A dominant coalition sets the scene overrides differences that are not in line with its logic and suppresses other perspectives wrote Brett and Janssens in the journal Group amp Organization Management 31 1 2006 This creates a less culturally intelligent team model because it discourages meaningful participation in information extraction and decision making The most common alternative approach the integration and or identity model requires all team members to sublimate their cultural identities to that of the entire team by adopting superordinate goals based on their common interests The approach has the advantage of encouraging every team member to participate However it carries two risks In the inter est of unity team members might submerge their cultural identities and hence their ability to think differently And the effort to include everyone in decision making might cause the team to function at the level of its least creative member In studies of the two approaches before and since publishing her original research We found problems with communica tion confrontation and commitment as well as norms for problem solving work behavior time urgency and pace and violation of norms for status and intergroup prejudices Those problems typically stem from the top One of the ways to get peo
185. y disrupt research Best Products Best Performance Best Protection Contact NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 USA Toll Free 1 800 328 3352 Phone 763 553 1270 E mail nugire nuaire com Website www nuaire com LOOKING FOR THE RIGHT PRODUCT WE CAN HELP le WWW LABMANAGER COM www labmanager com ADVERTISER INDEX Company A amp D Weighing Agilent Technologies Anton Paar Artel Beckman Coulter Inc BINDER Inc BioTek Instruments Inc BMG LABTECH BrandTech Scientific Inc Buck Scientific Conquer Scientific ELGA Environmental Sampling Supply Eppendorf North America EquipNet Inc Federal Equipment Company Flow Sciences Inc GenTech Scientific Inc Gilson Inc Helmer Inc Hettich Instruments LP INTEGRA LabAnswer Labconco Labnet International Inc Megalab Technology Mettler Toledo Inc NASMM NuAire Inc Panasonic Healthcare Parker Hannifin Corporation PHOTONIS PolyScience Proton Onsite Ruro Sigma Aldrich Co Sonntek Inc TECA Corporation Tecan Thermo Fisher Scientific Inc ULVAC Vistalab Technologies Waters Corporation WHEATON URL www andweighing com www agilent com chem CrossLab www anton paar com www artel usa com www beckman com www binder world us www biotek com www bmglabtech com www brandtech com www bucksci com ConquerScientific com www elgalabwater com www essvial com www eppendorfna com www EquipNet com www fedequip com
186. y research 50 to 150C AHP 1200CA5 With custom accessory wells Simple to use maintenance free reliable Cold Hot Plates Variety of plate sizes accessories and convenient features Dependable thermoelectric Peltier technology Temperature control within 1C Replaces ice baths assists histology applications and more 15 to 120C AHP 1200CPV Phone 773 342 4900 sales thermoelectric com www thermoelectric com teca Contact TECA today to learn more about the versatility of our laboratory cold hot plates Introducing Digital Water Baths at analog prices Large digital display i shows set and actual temperature simultaneously Built in timer eliminates need for anextradevice Y y Reach set temperature in a fraction of the time W PolyScience Temperature Control Solutions www polyscience com 1 800 229 7569 f Clear hinged cover tilts out of the way and lets condensation drain back into the bath while keeping samples in view y Handles make for I easy positioning and relocation of the bath oe Three user defined temperature presets canbeusedfor frequently used set temperatures 4 e Circulating Baths e General Purpose Water Baths e Chillers e Specialty Products Compared to previous models 34 TECHNOLOGY The Akron lab also uses both paper and digital record keeping methods
187. ysis Features rapid response and excellent accuracy Optimizes the unavoidable trade offs among measurement time dynamic range and signal to noise considerations for process monitoring measurements Ocean Optics www oceanoptics com Core Shell Biphenyl HPLC UHPLC Columns Kinetex Mi BOOTH 116 7 Give researchers excellent orthogonal selectivity to traditional 18 phases e Suited for a broad range of complex mixture analyses in clinical pa research and forensic toxicology food and environmental testing as A and pharmaceutical bioanalytical and DMPK studies e Deliver all the benefits inherent with Phenomenex s Core Shell Technology including better resolution higher efficiency sharper peaks and greater sensitivity than traditional HPLY UHPLC media Phenomenex www phenomenex com www labmanager com SRLS sacos Sum Space is limited REGISTER FOR FREE NOW gt WEDNESDAY MAY 14 2014 www labmanager com SRLS The LabX Media Group s Scientific Research and Leadership Summit SR amp LS is a suite of unique interactive programs hosted in virtual pavilions dedicated to Lab Management and Life Science Research The interactive webinars will offer a forum for education and information exchange among research scientists principal investigators lab managers directors postdocs and other lab professionals All attendees will have the opportunity to pose questions to industry experts and to browse through

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