Hardware User`s Manual Rat paw pressure meter
Contents
1. 7 2 LE 7306 REAR PANEL MOTOR REMOTE RS 222 POWER LE 7306 Doowoooo 0000000 Made in Spain Figure 7 LE 7306 Rear Panel e POWER Main switch power inlet and fuse holder e MOTOR Connection between the control unit and the stimulation unit e REMOTE Consists of the foot switch while pressed it activates the experiment Once there is an animal response the foot switch must be released and the rounded point will return at its maximum speed and to the original position e RS 232 For communicating between the computer serial port and the control unit Data is sent to the computer and recorded with the Sedacom software not included should be purchased separately Data is sent to the computer each time the foot switch is released Rat paw pressure meter 10 I Harvard Pa n la b H Apparatus 7 3 STIMULATION UNIT The stimulation unit allows a gradual increase at a selectable rate of the force applied to the member being studied This force is applied through a conic point that presses the animal s limb lying on a platform underneath the point This point is moved down increasing force and up releasing stimulus by a motor controlled with a pedal Figure 8 Stimulation Unit This conic point is mounted over an extensiometric cell a strain gauge that makes possible the determination of the real force expressed in grams that is actually being exerted by the stimulation point Figure 9 Two kinds of2. If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Rat paw pressure meter Panlab 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipment does not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next Use e The user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT In case of an over voltage or other incident in the AC net making it impossible to turn on the equipme
3. This is a switch that allows the stimulation point motor s speed selection and consequently the increase rate of the stimulation force used on the limb being studied Said speed can be increased from 1 to 4 The value selected only affects the speed at which the point moves when lowering The speed at which the point is raised is always at maximum speed e MEMORY This switch makes it possible to select the mode in which the force values are expressed on the FORCE display When the switch is in the OFF position the value shown on the display is the current value of the force being recorded by the transducer In this position changes in force are NOT memorised When this switch is in the ON position the value shown on the display is under the control of the starting pedal in such a way that its initial value is o zero When the start pedal is pressed its value will be progressively modified according to the tension being recorded When the pedal is released the display maintains the last value reached and the motor moves at its maximum speed in counter clockwise direction raising the conic point and thus releasing the stimulus over the paw This cycle is reinitiated when the pedal is pressed again Rat paw pressure meter Panlab Md e ZERO ADJUST This makes it possible to adjust the display value to zero when the transducer is free of charge To operate this control it is recommended that the MEMORY switch be in its OFF position
4. Depth 232 mm x 111 mm x 297 mm Weight 3 65kg Rat paw pressure meter 18 Panlab H DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direccion del fabricante Energia 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto ANALGESY METER Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 7306 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Subst
5. keeping pressed the pedal and when the point returns to the rest position when you release the pedal The force g being exerted at the moment the start pedal is released is considered the endpoint of the test Once the pedal is released the motor will turn counter clockwise raising the point at its maximum speed The value of the tension reached will remain on the display until a new trial is initiated by pressing the start pedal again Rat paw pressure meter 13 Panlab Md WARNING the endpoint of the test is established by the experimenter and NOT detected automatically by the instrument 9 2 CLEANING THE EXPERIMENTATION UNIT To clean the surface of experimentation unit you can use a lightly wet cloth and then dry it with a dry cloth If it s too dirty you can wet the cloth with a soapy solution then remove foam with a wet cloth and finally dry it with a dry cloth WARNING In order to clean the Perspex parts never use neither alcohol nor alcoholic derived products otherwise stripes will appear in the plastic Rat paw pressure meter 14 Harvar Panlab Harvard 10 WORKING WITHTHE SEDACOM SOFTWARE The purchase of the Sedacom software option is needed for transferring the data to a computer please contact your local sales delegate for more information The Sedacom software reference is composed by a USB Flash key containing the software Installer License for use and Sedacom User s Manual Follow next instruc
6. points WARNING The point can cause damage if too much pressure is applied be careful when handling the device Rat paw pressure meter 11 Panlab Horvord 8 EQUIPMENT CONNECTION The following schematic shows a sample equipment connection Figure 10 Equipment Connection The connections and necessary cables are listed in the following table FROM CABLE LE 7306 Motor DB1s cable LE 7306 Remote Jack mono 6 35mm LE 7306 RS 232 PC Com Port RS 232 cable Rat paw pressure meter 12 Panlab hard WORKING WITH THE EQUIPMENT 9 1 CONDUCTING AN EXPERIMENT Connect the cables as explained in Chapter 8 Activate the start control If necessary set the display to zero To do so Off Zero Adjust switch MEMORY to OFF and adjust the display value by using the ZERO ADJUST control until zero Mem O is reached Return the MEMORY switch to the ON DR position Select the speed of the tip with the SPEED knob 1 is the lowest and 4 the highest Situate the animal limb to be studied on the platform under the stimulation point so that this is at a distance of 5 mm Once the animal is prepared activate the start pedal The Force gr values on the display will progressively increase Once the selected analgesic response has been reached shaking of the stimulated limb vocalisation etc release the start pedal The RUN led remains on while the motor is on while the point is Run moving down by
7. seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente pa Nota em Ambiental Ap s a implementac o da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instru es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribuic o para a protecc o do ambiente D Rat paw press
8. Hardware User s Manual Rat paw pressure meter Randall Selitto test References LE7306 76 0234 Version V29 10 2014 Panlab s l u International Calls 34 934 750 697 Pan H Harvar d C Energ a 112 Domestic Call 934 190 709 ppararus 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Spain Info panlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab H 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can omega e exposed to a hazard Protection terminal ground connection D Warning about a hot surface which temperature may exceed 65 C AN touched Fr Y operative life Waste Electrical and Electronic Equipment Directive WEEE T 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good labora
9. Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits A For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category Il over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Rat paw pressure meter Panlab Hever PC Control Some of these instruments are designed to be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions
10. ances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mecanica Machinery directive Directive m canique Para su evaluacion se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2012 Class B FCC FCC47CFR 15B Class B Safety of machinery EN ISO 12100 2010 En consecuencia este producto puede incorporar el marcado CE y FCC Consequently this product can incorporate the CE marking and FCC C En consequence ce produit peut incorporer le marquage CE et FCC En representacion del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canalis mmm General Manager a Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 25 06 2014 Rat paw pressure meter 19 Panlab Ha bem Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting
11. l stimulus is one of the most recurrent problems in pharmacological research Multiple techniques have been proposed to manage this problem with the so called Randall amp Selitto test being one of the most widely used in routine determinations of animal nociceptive responses Figure 5 LE 7306 Analgesy meter In general terms the Randall 8 Selitto test is based on the determination of the response threshold to painful stimulation through pressure on the rodent s paw Normal animals as well as animals with inflammation induced by any of the available methods can be tested this way The LE 7306 Analgesy Meter is an instrument conceived to allow a rapid and accurate study of the analgesic activity using the Randall 8 Selitto test Randall L O amp Selitto J J Arch Int Pharmacodyn Ther 111 409 1957 Rat paw pressure meter Panlab jeg 7 EQUIPMENT DESCRIPTION 7 1 LE 7306 FRONT PANEL RUN FORCE MEM SPEED ZERO ADJUST Forte gr Zero Adjust ANALGESY METER LE 7306 Sy de p HARVARD APPARATUS Panla Figure 6 LE 7306 Front Panel e RUN This is a yellow led that remains on while the stimulation unit is activated e FORCE gr This is a digital display showing the force in grams marked by the strain gauge over which the stimulation point has been mounted This value is recorded The way the force value is expressed depends on the position of the MEMORY switch see paragraph MEMORY e SPEED
12. nement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique D Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt pa Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le
13. nt check fuses according to the following procedure 1 Remove power cord from the power inlet Rat paw pressure meter Panlab H Ade 2 Open fuse holder by pulling the flange with a regular screwdriver 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert again fuse holder both possible positions are correct because power supply is universal 6 Ifthe fuses blow again unplug the equipment and contact technical service For electrical safety never open the equipment The power supply has dangerous voltages WARNING Rat paw pressure meter Panlab 5 TABLE OF CONTENTS 7 2 7 3 9 9 1 9 2 10 11 12 13 SYMBOLS TABLE GOOD LABORATORY PRACTICE UNPACKING AND EQUIPMENT INSTALATION MAINTENANCE TABLE OF CONTENTS INTRODUCTION EQUIPMENT DESCRIPTION LE 7306 FRONT PANEL LE 7306 REAR PANEL STIMULATION UNIT EQUIPMENT CONNECTION WORKING WITH THE EQUIPMENT CONDUCTING AN EXPERIMENT CLEANING THE EXPERIMENTATION UNIT WORKING WITH THE SEDACOM SOFTWARE TROUBLESHOOTING PREVENTIVE MAINTENANCE TECHNICAL SPECIFICATIONS Rat paw pressure meter Harvard Apparatus 10 11 12 13 13 14 15 16 17 18 Panlab H Harvard 6 INTRODUCTION The quantification of an animal reaction to a painfu
14. points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment taf Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales a otras formas de reciclaje de usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente gt lt Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environ
15. re 10 Check that MEM switch is in the ON position If it is in the OFF position will not send data Make sure that both the serial port as the device selected in the Sedacom program are correct The equipment will send data only when you release the pedal then the tip is removed to the maximum speed The equipment does not send data to the program Sedacom This is because the transducer is slightly unbalanced You can balance it using the ZERO ADJUST adjustment with the help of a small screwdriver see point 3 of Chapter 9 The LE7306 screen shows a value different to ooo when the MEM switch is OFF position Rat paw pressure meter 16 Panlab Md 12 PREVENTIVE MAINTENANCE EXPERIMENT CLEAN EXPERIMENTATION UNIT SURFACE CHECK CABLES CONNECTION Rat paw pressure meter 17 Panlab 13 TECHNICAL SPECIFICATIONS Harvard Apparatus POWER SUPPLY Input voltage Frequency Fuse Maximum Power Conducted Noise Universal 100 240VAC 50 60 Hz 2 fuses 5mmx20mm 2A 250V fast 18W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating Temperature Operating Relative Humidity Storage Temperature 10 C to 40 C 0 to 85 RH non condensing o C to 50 C non condensing FORCE Range o gr to 999 gr Resolution 1gr COMUNICATIONS OUTPUT Standard Interface RS232C Connector Delta 9 contacts female connector DIMENSIONS Width x Height x
16. tions e Please refer to the Sedacom User s Manuel for the instruction about how to install and use the software with the present device e A serial port RS232 communication cable provided with the present device is needed for the connection of the present devices to the computer in which the Sedacom software is installed Please refer to the present User s Manual for the instruction about how to connect this cable to the device e Ifthe computer doesn t have any serial port the RS232 USB adapter is needed ref CONRS232USB contact your local sales delegate for more information WARNING the RS232 communication cable provided with the device is used for any connection of the device with associated software Sedacom etc When the device is used without software in first instance this cable needs to be preserved and kept in a secure place in case the need of using the system with a software arises in the future In this last case if the user lost the cable a new one should be purchased to his local sales delegate ref CONRS232 The warranty time of this cable is the same than the warranty time of the device Rat paw pressure meter 15 Panlab H Mae no 11 TROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION The equipment does not start up e Check the condition of the fuses Check that the cable is connected between the RS 232 device and the PC serial port see Figu
17. tory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Rat paw pressure meter Harvar Panlab Harvard 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments
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