SC-300 Pro Kit Manual
Contents
1. 2 meq mmol SO2 S mL SC 300 Pro Kit Manual 14 Version 2 1d Measuring Total SO by Titration optional requires 1N NaOH l Place 25 mL wine or must in the titration vessel See Figure 17 2 Add 10ml 1N sodium hydroxide Vinmetrica Part number SC 100 17 and mix well Let stand approximately 10 minutes 3 Using the transfer pipettes add approximately 8 mL of the Acid Reagent and 2 mL of the Reactant solution to the vessel Remember that if you are using the transfer pipettes in the kit 2 mL is the amount that fills the bulb with a vigorous squeeze so dispense four of these for the Acid Reagent 4 Proceed from step 5 in the Free SO procedure above The result calculated will be total SO rather than free SO in parts per million ppm or mg L Calibration of pH 1 Be sure the pH electrode is attached to the unit via the BNC connector Then select CAL mode by pressing the MODE button until the CAL LED illuminates 2 Choose a calibration set that corresponds to the range you are working in Usually for wine this will be at pH values below 4 so use the 4 7 set If you have a source of a pH 3 00 reference solution you can use this in place of pH 4 3 Rinse the electrode with DI water shake or blot off excess liquid gently and place the electrode into a small vessel like the pH 4 Reference Solution cap containing one of the reference solutions e g pH 4 01 4 The instrument will determine which calibration s2. Vinmetrica SC 300 Pro Kit User Manual Oinmetrica Vinmetrica SC 300 Pro Kit is a simple and robust device that provides high accuracy in determination of sulfite SO pH and titratable acidity TA levels in wines ciders and other liquids These are essential parameters to control in the effort to make high quality wines The Pro kit includes lab accessories for the SC 300 Analyzer Table of Contents Materials Provided in the Kit cccccccccccccscescescescsscsscscsecsesscsscsscsscsscsscsessessessessessessesececusussessesseeess 2 Thines yon W AA essaten E TAa E a dea taste EA E E 2 Why Test for SO2 pH and TAP cece cccccccsccsscsecccsscsscsscsscsscsscsccscsssssessessusssssssssssssscsscssessesssseeeeess 2 Theory of Operation ccccccccccccceccsccsscssccsscsscssccsscsecssccsecsecssesssssscssscsscesccssssuessesssssscsusesesseceeesessseeess 4 SE EIT a coca Scene eet te sere et nate he ect ete ee eee 5 Setting up the SC 300 for the first time 0 ssseoeeneesesssseeeersssssssseerrssssssssseeersssssssseeersssssssees 5 Assembiine the Pro Kit EQuipme nl eesse 6 Burette Maintenance c ccccccccccsccsscssesscsscsscsscscsscsscssessessecsecsecsscescsscsscsessecsessessecseceuceessesees 8 Bonte Redio ap E eo ee ee ee ee een ee eee eee 9 Instrument Operation 0ccccccc cece ccc ceccssccescesccssecsscsecssccsscssccssccssssecssscsscssecssscsesesesssesssseseneees 10 Proce dieS secs A sa oe sect eg ht oes xe E E wie oatpraeansee
3. and the reactant and swirl in the usual way keeping constant motion The instrument may or may not indicate STOP as above If it does not add a drop of the SO Titrant solution This should make the STOP condition occur If it doesn t you may have an electrode problem 4 Now add one drop of KMBS solution 1 10 is fine swirl and verify that the STOP signal ends and the PROCEED light illuminates If this is the case your electrode has been conditioned If the electrode is still hot and the PROCEED light does not illuminate let it sit in 20 mL of distilled water with a half bulb full of the acid solution for a few hours Now repeat the test from step 3 If it works great If not call us and we will try to troubleshoot or replace your electrode Technical assistance info vinmetrica com tel 760 494 0597 SC 300 Pro Kit Manual 28 Version 2 1d
4. before beginning the pH amp TA combined measurement You can use a clean 5 mL sampling pipette provided in the kit to pipette this amount Measuring Titratable Acidity TA by Titration l If you are working with a sample of must we recommend homogenizing your sample in a blender before proceeding otherwise your TA values can be very inaccurate Take 100 mL or more of your must and put it in a blender on high for 30 seconds Allow solids to settle for 2 minutes before sampling or use a cheese cloth or mesh strainer to remove solids 2 Fill the syringe by drawing up the TA Titrant 0 133N NaOH Expel bubbles and set the plunger on the syringe to a readable point preferably the 5 0 mL point Note the 5 0 mL setting allows determination of up to 10 g L TA in a standard 5 mL wine sample If you are using the burette use the syringe to dispense the TA titrant into the top of the burette Make sure the burette stopcock is in the closed the red handle is horizontal position When filling the burette make sure the TA titrant has completely filled the bottom of the burette including the tip Sometimes bubbles can be trapped in the tip of the burette but can usually be dislodged by opening and closing the stopcock while the burette is above a waste container If you spill any TA titrant on the outside of the burette be sure to clean it up with a paper towel or dry rag If the spilled titrant is not cleaned from the outside of the burette you may in
5. make the instrument appear to be insensitive or unresponsive For SO2 measurements with the SC 300 there are several quick tests you can do to make sure the instrument is not faulty l Be sure the battery is good per the manual s instructions 2i Remove the electrode to expose the BNC connector at the back of the instrument Turn on the instrument and select SO mode Short out the terminals on the connector using a paper clip or similar metal piece to touch the center pin of the connector to its outer metal sheath The device should indicate STOP with its red LED and buzzer or beeper If this does not happen there may be a problem with the instrument contact us for more information 3 Connect the electrode and put it in about 20 mL of distilled water add about 1 ml half a bulb squeeze of each of the acid solution and the reactant and swirl in the usual way keeping con SC 300 Pro Kit Manual 27 Version 2 1d stant motion The instrument may or may not indicate STOP as above If it does not add a drop of the SO Titrant solution This should make the STOP condition occur If it doesn t you may have an electrode problem read in the next section below how to fix this Now add one drop of a concentrated sulfite solution 1 10 is fine and verify that the STOP signal ends and the PROCEED light illuminates If this happens your electrode is probably OK as well 4 Finally you can check your SO reagents with the ascorbic acid v
6. of baking soda sodium bicarbonate with stirring until effervescence ceases Technical assistance info vinmetrica com tel 760 494 0597 SC 300 Pro Kit Manual 19 Version 2 1d WARRANTIES AND LIABILITIES l The materials provided in the kit as described on pages 1 and 2 above Materials are warranted as follows The SC 300 instrument SO electrode and non reagent accessories are warranted against defects in workmanship for 24 months from date of purchase The reagents are warranted to perform as described herein up until any stated expiration date or 6 months after purchase whichever is later The pH electrode is warranted for 12 months THE WARRANTIES IN THESE TERMS AND CONDITIONS ARE IN LIEU OF ALL OTHER WARRANTIES EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION ANY WARRANTIES OF MERCHANTABILITY NONINFRINGEMENT OR FITNESS FOR A PARTICULAR PURPOSE SAID WARRANTIES BEING EXPRESSLY DISCLAIMED 2 Buyer agrees that its sole and exclusive remedy against Vinmetrica shall be limited to the repair and replacement of Materials or parts of Materials provided Vinmetrica is promptly notified in writing prior to the expiration of the warranty period specified above of any defect Vinmetrica s liability for any damages due Buyer shall be limited to the purchase price of the Materials 3 VINMETRICA S MAXIMUM LIABILITY FOR ALL DIRECT DAMAGES INCLUDING WITHOUT LIMITATION CONTRACT DAMAGES AND DAMAGES FOR INJURIES TO PERSONS OR PROPERTY W
7. value is within 0 5 pH units of the target but the CAL light fails to flash in the expected ready to calibrate manner you can try the force calibrate feature available in firmware 3 0 6 and higher described in the manual under Calibration of pH 6 If the displayed pH value is outside of the default tolerance of 0 5 pH but not more than 1 5 pH units you can change the baseline of the pH value See Test Mode stage 14 in Appendix A Call or Email us for help if you need it T Finally refer to the next FAQ question 1f these steps do not help SC 300 Pro Kit Manual 24 Version 2 1d What should I do if my pH electrode is acting sluggish erratic and is difficult to calibrate Reconditioning and cleaning of pH electrodes Even in normal use and storage performance of pH electrodes may show deterioration over time which typically shows up as noisy erratic or sluggish electrode readings and or difficulty calibrating Assuming the meter itself 1s working see Meter test below then there are two main causes for this 1 Clogging of the reference junction most likely 2 Fouling of the glass membrane happens occasionally or after prolonged service The following procedures will often provide renewed stability and pH sensitivity If the electrode cannot be restored by one of these methods it needs to be replaced Unblocking the reference junction The reference electrode junction is usually the problem
8. voltage source Do alternating parts First the integer not exceed 0 5 V portion of pH level is shown 1 to 14 Next three decimal places are shown Readings above 14 00 are shown as Readings below 0 00 are shown as pH 4 SO SO probe or SO The SO current in nanoamperes 1s probe simulator e g displayed For values under 10 one 500 kOhm resistor decimal place is shown 5 pH Voltage pH probe or precision The raw voltage output from the voltage source Do instrument s pH amplifier is displayed as not exceed 0 5 V X XX volts Readings can range from 0 00 to 4 10 SC 300 Pro Kit Manual 21 Version 2 1d a Seen 6 SO Voltage SO2 probe or The raw voltage output from the SO probe simulator instrument s current amplifier is displayed as X XX volts X XX if less than 1 00 Disconnect probe cycled through its 32 levels Note Ignore red LED error indication 8 Battery Install two AA The battery voltage is displayed as X XX Voltage batteries volts 9 Character Set None Every ASCII character space to is 7 DAC Test None The Digital Analog Converter DAC is displayed Due to the limitations of the 7 segment format some characters are not used by the software 10 Number None The display cycles through showing Display every possible digit and every decimal point s 11 Sound Test The beeper is turned on continuously 12 pH CAL Displays current CAL values for pH 7 v
9. when the electrode can t calibrate in its expected ranges This junction is a fine pored frit that allows electrical contact of a reference electrode with the solution being tested It can become clogged over time l Soak electrode in hot NOT boiling water about 60 C for 5 10 mins Allow to cool to room temperature then place in pH 4 reference solution for 5 minutes Try to recalibrate If this does not work try remedy 2 2 Place the pH electrode into the pH storage solution available from Vinmetrica part number SC 200 10 or a solution of 2 5M KCl with optionally added 0 01M KHP at 60 C and allow electrode and solution to cool to room temperature then place in pH 4 reference solution for 5 minutes Try to recalibrate If this doesn t work try remedy 3 3h Soak in 0 1M HCI note this can be made by diluting 1 mL of the SO Acid Solution with 20 mL DI water or 0 1M nitric acid HNO3 for 1 hour Rinse with DI water then place in pH 4 reference solution for 5 minutes Try to recalibrate If this does not work try remedy 4 4 Soak in 1 10 dilution of bleach in a 0 1 0 05 solution of liquid detergent in hot water with vigorous stirring for 15 mins Rinse with DI water then place in pH 4 reference solution for 5 minutes Try to recalibrate Cleaning the pH electrode s glass membrane The glass bulb is a thin membrane of a special kind of glass that actually does the job of responding to the pH of the solution It ca
10. 0 3 blue polycarbonate housing 3 pH Electrode Part number SC 200 3 red or blue polycarbonate housing Two 5 mL syringes Two 3 mL polyethylene transfer pipettes One 25 mL serological sampling pipette Figure 1 The SC 300 instrument One 5mL serological sampling pipette 100 mL polypropylene titration beaker SO Reagent set Part number SC 100 2 SO Titrant solution 0 0156N Acid reagent Reactant solution 10 pH TA reagent set Part number SC 200 8 pH 4 01 Reference solution pH 7 00 Reference solution TA Titrant 0 133N NaOH 11 Lab Support stand the large blue metal base with its long metal rod Part number SC 300 3 12 Vinmetrica Electrode Holder Part number SC 300 8 13 Double Burette Clamp Part number SC 300 6 14 10 mL or 25 mL Glass burette with Teflon Stopcock with 2 grams of Burette detergent Part number SC 300 7 LS Magnetic Stirrer includes two AA batteries stir bar and 25 mL cylindrical container Part number SC 300 4 16 Rinse Bottle Part Number SC 100 17 ee Ss Things you will need Two standard AA batteries alkaline type 2 Distilled water which usually can be found at your local grocery store It s handy to have a wash bottle for rinsing This bottle 1s now included in the Pro Kit as of January 2015 or available from Vinmetrica Part number SC 100 17 3 Optional IN Sodium Hydroxide solution Gf you want to do total SO2 Available from Vinmetrica Part number SC 100 7 S
11. C 300 Pro Kit Manual 2 Version 2 1d Why Test for SO pH and TA Testing for sulfite SO2 is crucially important for making sure your wine does not spoil by oxidation or from microbial growth Having the proper SO level in your wine gives you this protection By monitoring your SO levels you can make adjustments when needed especially before starting primary fermentation after malolactic fermentation has completed after racking or when ready to bottle To correctly adjust sulfite you need values for your current Free SO level and your wine s pH both of which can be measured with the Vinmetrica SC 300 analyzer The key parameter in protecting your wine is Molecular SO2 which for most wines should be at 0 8 ppm mg L following primary fermentation This in turn depends on the Free SO it can also be referred to as unbound SO and the pH Overall you can reach your target Molecular SO2 by measuring and adjusting your free SO levels and considering your wine s particular pH See Table 1 Table 1 Free SO concentrations necessary to attain 0 8 mg L Molecular SO at a designated pH FreeSO2 ppm 13 16 21 26 32 40 so 63 79 99 125 We recommend using a sulfite calculator for determining how much sulfite to add to your wines after taking a sulfite measurement with the SC 300 Pro Kit Winemaker Magazine s Sulfite Calculator at http winemakermag com guide sulfite can walk you through the process See Appen
12. HETHER ARISING FROM VINMETRICA S BREACH OF THESE TERMS AND CONDITIONS BREACH OF WARRANTY NEGLIGENCE STRICT LIABILITY OR OTHER TORT WITH RESPECT TO THE MATERIALS OR ANY SERVICES IN CONNECTION WITH THE MATERIALS IS LIMITED TO AN AMOUNT NOT TO EXCEED THE PRICE OF THE MATERIALS IN NO EVENT SHALL VINMETRICA BE LIABLE TO BUYER FOR ANY INCIDENTAL CONSEQUENTIAL OR SPECIAL DAMAGES INCLUDING WITHOUT LIMITATION LOST REVENUES AND PROFITS HAZARDS AND TOXICITY All Materials offered by Vinmetrica are intended for use by individuals who are familiar with laboratory procedures and their potential hazards The Materials contain chemicals which may be harmful if misused Due care should be exercised with all Materials to prevent direct human contact Glassware can break and chemicals can splash during experiments Always use safety glasses We strongly recommend using nitrile or latex gloves and wearing long pants long sleeves and closed toed shoes Keep out of reach of children Viametuca 1945 Camino Vida Roble Suite Carlsbad CA 92008 www vinmetrica com 760 494 0597 info vinmetrica com Copyright 2010 14 Sportsman Consulting LLC DBA Vinmetrica All rights reserved SC 300 Pro Kit Manual 20 Version 2 1d Appendix A Test Mode Test Mode provides various special functions that may be useful in testing the device for example if troubleshooting is necessary Test Mode is entered when the POWER button is pressed longer than 2 second
13. alues firmware and 4 in mV Pressing ENTER cycles 3 0 6 and later between these If a pH CAL reset has been done displays CAL value for pH 3 rather than 4 13 pH CAL Displays PrESS EntEr Press ENTER reset to reset pH CAL parameters and DAC firmware 3 0 6 Index to default values Message Good and later CAL rSt then scrolls 14 pH DAC None only firmware Displays PH DaC Set then displays Set digital to v3 1 2 and later the DAC Index for pH bias voltage analog converter followed by the voltage value itself output Default is 16 range 12 20 Press ENTER to increase the DAC value by 1 press MODE to decrease by 1 Can be used to change the baseline pH value 15 SO Baseline None Disconnect SO Sets baseline value for SO2 mode Let electrode only fw message scroll 5 sec then press ENTER v3 1 0 and later 16 TA endpoint None only firmware Displays seT Ta PT then displays TA v3 1 2 and later endpoint value Press ENTER to toggle between default of 8 2 USA standard or 7 0 European standard SC 300 Pro Kit Manual 22 Version 2 1d Appendix B Sulfite and TA Adjustments Using the Winemaker Magazine Sulfite Calculator Winemaker Magazine s Sulfite Calculator at http winemakermag com guide sulfite is an excellent tool for calculating how much sulfite should be added to your wine We will briefly go over the process here for some clarification l Select a Preferred m
14. ant It is not necessary to be extremely accurate in this step with these pipettes 2 mL is roughly the amount that fills the pipette up to the 2 mL mark after a single thorough squeeze of the bulb To preserve the shelf life of these reagents take care not to cross contaminate the transfer pipettes If they do get contaminated rinse them off with distilled water and let air dry Caution the Acid reagent is corrosive and can cause damage to clothing skin and eyes The reagents should not be ingested ALWAYS use safety glasses We recommend the use of laboratory latex or nitrile gloves during this procedure If any solutions contact skin or eyes flush with plenty of water If you are using a magnetic stirrer turn it on to stir at a moderate rate The Magnetic Stirrer provided with the SC 300 Pro Kit operates at a suitable preset speed Make sure your electrode is not being struck by the spinning stir bar To prevent this we recommend using the Vinmetrica Electrode Holder to stabilize your electrode Rinse the electrode briefly with distilled water Insert the electrode into the titration beaker so that the tip is completely submerged to just above the circulation gaps cutouts at the tip of the electrode but above the level of the stir bar approximately half an inch from the bottom of the titration beaker If you are using the Vinmetrica Electrode Holder adjust it to a similar level If you are stirring manually begin now use a constant modera
15. brating the pH electrode which must be attached The display initially shows CAL for a few seconds as it prepares to read pH and lets readings settle Thereafter the display shows the measured pH level with two decimal places Calibration works with one of the following reference calibration sets pH 4 01 and 7 00 or 4 7 pH 7 00 and 10 00 or 7 10 pH 3 00 and 7 00 or 3 7 Vinmetrica recommends use of the pH 4 7 Reference Solution set provided with the kit The magnetic stirrer has two modes Pressing the light bulb button on the magnetic stirrer activates a light underneath the sample and the stirrer The power button activates just the stirrer After pressing either button the stirrer remains active for 60 seconds a feature to conserve its batteries If during the titration it turns off simply press the button again for it to continue We recommend using the light mode because it helps us indicate when the stirrer stops Plus when doing TA titrations watching the wine turn from deep red to dark green is cool Note When using the magnetic stirrer be sure that the electrode does not touch the spinning stir bar as there is a slight chance that it can damage the glass bulb of the pH electrode or the platinum wires of the SO electrode If you are using the Vinmetrica Electrode Holder adjust the electrode s height so that its probe end is above the level of the stir bar SC 300 Pro Kit Manual 11 Version 2 1d Procedures Mea
16. crew makes adjusting Burette Clamp slides pH electrode inserted into onto the Lab Support The Vinmetrica Electrode easily onto the Lab the Electrode Holder Stand Holder s height easy Support Stand to the wine sample Carefully open the Double Burette Clamp s spring arms with your thumb and forefinger and place the burette in between the four indentations in the double burette clamp s spring arm s plastic knobs See Figure 8 Slowly release the clamp spring to secure the burette between these four spring arm plastic knobs See Figure 9 You can adjust the burette at any time by raising and lowering it within the spring arm clamps of the Double Burette Clamp When making these adjustments hold onto the burette and do not apply too much force to it as the burette is delicate Use the end of the Vinmetrica electrode holder as a guide for the tip of the burette Do not try and force the burette into the cutout at the end of the electrode holder This may cause your burette to snap and break Finally insert the electrode that you wish to use into one of the open side slots as seen in Figure 11 Once the rubber neck of the electrode is sitting on top of the electrode holder you can press down on the rubber neck so the electrode is further stabilized into the slot You are now ready to titrate Note The magnetic stir bar that is placed within the 25 ml cylindrical container or titration beaker can potentially damage the SO and pH electrodes When a
17. dix B for more information on how to adjust your wine for sulfite Monitoring your wine s pH is also important for the first few months of the wine making process Proper pH and Titratable Acidity TA levels influence mouth feel and provide wine stability During malolactic fermentation the pH can increase somewhat and should be monitored Typically wine pH and TA are inversely related when pH goes up TA goes down and vice versa Adjustments may be made to your wine to prevent wine instability See Appendix B for more information on adjustments SC 300 Pro Kit Manual 3 Version 2 1d Theory of Operation lie Sulfite S02 The SC 300 with the SO electrode and reagents provided can be used to determine sulfite levels in wine musts and other samples It relies on the Ripper titration based on the quantitative reaction of the SO with iodine generated during the titration which oxidizes the SO in the sample under acid conditions IO 51 6H gt 3L 3HO generation of iodine In from iodate 103 SO bL H2O gt 2I S0O 2H reaction of SO and iodine When all the SO is titrated at the endpoint excess iodine appears in solution This is detected as current with the SO electrode and signaled by audible and visual indicators The endpoint is much more sensitive than the starch color change commonly employed for Ripper titration and it is sharp and clear even when titrating red wines and musts From the known concentrati
18. djusting the height of the electrodes make sure that the stir bar in the beaker is below the bottom of the electrode You do not want the spinning magnetic stir bar to strike the SO or pH electrode SC 300 Pro Kit Manual 7 Version 2 1d Figure 8 The glass Figure 9 The burette sits Figure 10 Use the end of Figure 11 The Pro Kit burette should be placed firmly between the the Electrode Holder asa Assembly is complete Go between two sets of notches of the four guide for the glass burette take some measurements spring arm knobs on the spring arm knobs of the for accurate titrations Double Burette Clamp Double Burette Clamp Burette Maintenance Keep the burette clean and wash with deionized distilled water when you are finished using the burette This is especially important for TA titrations because the TA Titrant is caustic and can etch the burette Every so often you will want to clean your burette with a cleaning solution to maintain accuracy of the burette The included 2 grams of Burette Detergent is specially formulated to clean glassware The dry detergent ships in an Eppendorf tube that has markings with approximate half gram increments Upon first receiving the detergent mix approximately half a gram about an 1 8th of a teaspoon with about 50 milliliters mL of distilled water into a small bottle We recommend capping the bottle and shaking well for a minute or until the detergent is dissolved Bubbles may form but the d
19. e to check your SO reagents If you are worried about your TA Titrant you can run the KHP test also located on the Vinmetrica website in the FAQ section under Manuals Tests and How To Videos at vinmetrica com FAQ I added the calculated amount of sulfite to my wine but the numbers are still low This is acommon occurrence with several explanations any or all of which may be happening l Make sure you are using fresh sulfite powder Potassium metabisulfite degrades over time and that stuff you bought 2 years ago is probably bad now 2 Make sure that you stir your wine thoroughly when you add sulfite If you pour a 10 solution of KMBS into your wine it sinks like a battleship A sample taken off the top will read low unless the wine is stirred 3 A significant portion of the sulfite you added may have ended up bound particularly 1f your free SO was very low to begin with This bound SO does not show up when you measure free SOz and it is not protecting your wine You will need to add more sulfite until your free SO2 comes up to the right level Sometimes you must add 2 or even 3 times more sulfite than you first calculated I m getting strange results in SO mode how do I know if my instrument is working correctly We recommend that you always start in SO2 mode with the electrode disconnected After pressing ENTER to confirm SO mode then connect the SO electrode This avoids incorrect back ground settings that may
20. erent from 7 00 but generally if this test passes it is much more likely to be an electrode problem Help What do I do if my pH electrode was attached to my SC 300 when in SO mode Is my pH electrode broken No don t worry your pH meter should still work To fix it leave your pH electrode in the pH 4 reference solution for an hour and it should return to normal function Enabling SO2 mode with the pH electrode attached could cause the pH electrode to wear out more quickly Because of its design SO2 mode cannot be accessed unless you press ENTER after pressing the MODE button this is another safeguard we installed to prevent this from becoming a problem Appendix C2 Troubleshooting SO Issues How stable are the reagents The SO reagents and the pH TA reagents are all warranted to last for 6 months We have found that in fact except for the TA Titrant our reagents are stable for well over 12 months if stored tightly capped out of the heat and direct sunlight And of course these reagents will last much longer if not SC 300 Pro Kit Manual 26 Version 2 1d cross contaminated with each other The TA Titrant can pick up atmospheric CO and lose potency over time so we recommend that you replace it every six months How can I check the accuracy of my reagents It s rare that the SO reagents go bad but if you are concerned about it run the Ascorbic Acid Test method located in the FAQ section of the websit
21. es 12 Measuring Free Sulfite SO2 by Titration cccccececccccescesccscsscssccsscssesscssscssessessssssessseesens 12 Measuring Total Sulfite SOz by Titration ccccccececccceccsccsscssessscsscesccsscssesscssssesesesseseees 15 Calibraion of PH ene en nt Pee E 15 IN Ve S190 Messe cece ata tncediecea dace geteo aapqnszatecteoed seatetasesatoienioe beeieadscas teas cusences auseqstentedeoed aces 16 Measuring Titratable Acidity TA by Titration cccccccccecccccescesccsscescescssscssessessssssesseseeeees 17 Finishing Up cccccccecccccesccsscsscesccsscsscssccsscssccsecsscsccsscsscsscssscssecssssucsssssecsucsssssssesssssusesscsseesees 19 Warranties Liabilities amp Hazards cccccccccccscccescccssccceescceecccscccscecscecscecsuseceesecesseceeseeeseeess 20 Appendix A Test Mod e cccceccccccceccssccsccssccssccsecsccsscesecssccsscssscssscssscsssesecsuscssssecsuecssessecsueeasens 21 Appendix B Sulfite amp TA Adjustments 0 000 0cccccc cece ccscesccesccsscesecssccesesecssecsussesssesaseseees 23 Appendix C1 Troubleshooting pH and TA Issue c0cccccceccceccecesccsscesscssecssessscesessseeseens 24 Appendix C2 Troubleshooting SO ssues 0 0ccccccccccecesscescescescesscssscssecssesssessssssesssesseeees 26 US Patent pending SC 300 Pro Kit Manual 1 Version 2 1d Materials provided in the kit 1 Vinmetrica SC 300 SO2 pH instrument Part number SC 300 1 2 SO Electrode Part number SC 10
22. etergent will still be effective The detergent solution should be made fresh every time you clean the burette as it loses its potency quickly if stored as a liquid After you have run distilled water through your burette close the stopcock and fill with the Burette Detergent solution You can open and close the stopcock over a waste bucket or sink to allow the solution to pass into the tip of the stopcock We recommend letting this detergent solution sit in the burette for one hour Dispense the detergent solution then rinse the burette with distilled water twice more During this time we recommend opening and closing the stopcock to make sure the stopcock is turning easily If it is not remove the stopcock and clean it thoroughly Once drained allow to hang dry by hanging the burette upside down with the stopcock in the open position Once the burette is dry flip it back over and cover the top opening with a piece of tape This will prevent dust from getting in that can cause aberrations when reading the burette Remove the tape when you re ready to titrate SC 300 Pro Kit Manual 8 Version 2 1d Burette Reading ALWAYS use eye protection and preferably gloves latex or nitrile when using glassware and chemical reagents To get the most accurate results when titrating there are a few things to keep in mind We recommend reading from the bottom of the meniscus See Figure 13 on the following page First use a thick sheet of white paper note ca
23. ethod of Sulfite addition we recommend using a 10 solution of Potassium metabisulfite KMBS You can prepare this solution by weighing out 10g of KMBS and dissolving it in a FINAL volume of 100 mL DI water 2 Next choose the wine type red or white 3 Keep the Desired Molecular SO at 0 8 mg L and enter your wine s pH value hit your keyboard s Enter button after entering the value 4 After entering in your Desired molecular SO and your wine s pH the calculator will ask you to enter the Desired level of free SO The calculator already knows what this value is based on the data you entered and it should appear in the Notes section below the entry fields but the one fault of this app is that at least when we run it it won t tell you until you go through the calculation once So for now just enter 100 for the Desired level of free SO2 and proceed you will come back to this step in a moment to get the right answer see below 5 Now input the Current level of Free SO2 which you determined from measuring Free SO with the Vinmetrica SC 300 6 Finally enter the volume of wine to be corrected Choose liters or gallons we prefer liters because the answer is returned in mL The Amount of sulfite to be added should be in mL or fluid ounces of 10 sulfite solution or in grams if you use sulfite powder as your sulfite additive J Now press the blue Calculate button Ignore the answer belo
24. ette stopcock valve you first start You are titration The red STOP now ready to titrate LED will also remain lit 10 During the titration the LCD display will show transient currents the red STOP LED will briefly illuminate and the beeper will sound beep beep These transient indicators will last longer and longer as you approach the endpoint Figure 22 Take the endpoint as the first addition of Titrant that causes the display to exceed 50 and the red LED and beeper to stay on for longer than 15 seconds or a count of 20 sets of beep beep It is important to maintain stirring or swirling to detect the endpoint well Do not add titrant while the red STOP LED is lit Read the remaining titrant volume off of the syringe or burette 11 Calculate the volume of titrant used V using the Syringe Starting volume minus final volume Burette final volume minus starting volume e g V 5 0 mL 3 5 mL 1 5 mL 12 The free SO content is calculated in units of parts per million ppm or mg L as 64 V N 1000 ppm mg L Free SO DS Where V mL SQ Titrant needed to reach the endpoint N normality concentration of the Titrant and S mL of your wine sample Tf you use a 25 mL wine sample as directed and the SO Titrant s normality is 0 0156 as supplied in the kit then the calculation is simply ppm mg L Free SO 20 V i e 20 times V 1 64 mg SO2 mmol SO2 V mL N meq mL 1000 mL L
25. in the figures below These photos are of the 10 mL burette the 25 mL burette will have fewer gradations Please take a look at your burette and determine the values for the gradations lines i eee Iu jen Figure 12 On a thick white Figure 13 The Meniscus of the Figure 14 Reading the Meniscus of sheet of paper or a business card water column This curve is the water column is easier and more use a black marker to draw a formed because of interactions precise if you use the paper with the band approximately an inch and of water with the glass burette black band held up an inch behind the a half thick This card will assist We recommend measuring the burette and its black band 1 2 an inch you in reading the burette titrant volume from the bottom below the meniscus In this picture the accurately of the meniscus In the picture value from Figure 17 is further the value is about 3 27 mL resolved to 3 26 mL SC 300 Pro Kit Manual 9 Version 2 1d Before beginning the titration record the starting titration volume using the thick white paper with the black band and then begin titrating slowly With enough practice you will eventually be able to read the burette without black banded paper Record the final titration volume using the same technique You can also checkout these websites for more burette info http www titrations info pipette burette or http www csudh edu oliver demos buretuse buretuse htm Instrument Operati
26. ing motion If the electrode is not held in a stand hold it against the side of the vessel with one finger and grasp the vessel with the remaining fingers so that the two move together while swirling See Figure 23 l Figure 23 Manual stirring Figure 24 Automated Figure 25 Make sure that Figure 26 Once the pH technique Hold the stirring technique Turn on the green PROCEED arrives at or passes 8 20 electrode against the side of the magnetic stirrer add LED is lit You should be you are done with the the titration beaker and TA Titrant by slowly reading a pH close to what titration The red STOP swirl gently add TA Titrant opening the burette you expect your wine is at LED will be lit and the with other hand stopcock valve You are ready to titrate instrument will be beeping 8 Verify that the pH is less than 7 and the green PROCEED LED is lit see Figure 29 If the pH is greater than this and or the red STOP LED is lit and the beeper sounds your sample has less than 0 1 g L TA and there is no need to proceed 9 Titrate the sample by adding the TA Titrant drop wise from the syringe or burette being sure to note the starting volume mark on the syringe or burette During the titration the pH will gradually rise from its starting value below 4 usually As you approach pH 7 go slowly in adding successive drops of titrant so as not to overrun the endpoint Be sure to mix thoroughly after each successive drop of
27. ised metal inserts then close the housing If desired you can prop the unit up using its folding stand Low Battery Detection When the battery level is getting low the instrument will show a low battery icon on the upper left side of the display but continues to operate without impairment to any function Replace the batteries as soon as practicable When the battery level drops too far the instrument does not operate It rapidly flashes the low battery icon for 3 0 seconds beeps and shuts itself off Auto Shut off The SC 300 shuts off after 30 minutes If this happens unexpectedly just press the POWER button to resume from where you were Electrodes When directed to do so attach the desired electrode SO or pH Figures 2 amp 3 via the proper connector protruding from the top on earlier model SC 300s there is just a single connector for both electrodes Be sure to secure the electrode plug to the BNC connector to insure proper function Figure 2 Attach the SO2 Figure 3 Be sure the pH electrode to the phono plug Electrode attachment is screwed connector on the SC 300 by into place on the BNC pushing it straight on Avoid a connector twisting motion SO electrode Remove any protective cover from the electrode tip some electrodes are shipped without one This cover is for shipping protection and need not be used routinely Put the electrode on its side or hang it from the electrode holder if you have one The SO elect
28. itamin C test located on our website vinmetrica com FAQ Preconditioning of the electrode Note as of October 2013 all SO2 electrodes are shipped pre conditioned so this procedure should not normally be needed Sometimes when the SQ elec trode is first shipped it can be shipped hot A hot electrode is one that is overly sensitive as indicated by high readings and STOP conditions when inserted into solutions that should be giving no signal like pure water or water plus reactant and acid with a drop of 10 KMBS added If your electrode shows this behavior it generally can be fixed by pre conditioning as follows l First turn the power on and press the MODE button until the instrument is in SO2 mode then press ENTER Attach the SO electrode to the SC 300 analyzer Put 20 mL of distilled water deionized water in a beaker and add half a bulb squeeze of the acid solution half a bulb squeeze of the reactant solution and let the SO electrode sit in this solution 2i Then add one drop of 10 potassium metabisulfite solution KMBS to the beaker with the electrode in it Swirl gently The instrument LCD should now read 0 0 in units of nanoamps or close to it If it is reading significantly higher than 20 on the screen let the electrode sit in the solution for half an hour 3 After half an hour rinse the electrode with distilled water Put it in about 20 mL of distilled water Again add half a bulb squeeze of each of the acid solution
29. izontal When filling the burette make sure the SO titrant has completely filled the bottom of the burette including the tip Sometimes bubbles can be trapped in the tip of the burette but can usually be dislodged by opening and closing the stopcock while the burette 1s above a waste container If you spill any titrant on the outside of the burette be sure to clean it up with a paper towel or dry rag If the spilled titrant is not cleaned from the outside of the burette you may introduce these spilled titrant droplets into the wine sample leading to an inaccurate reading Be sure to record your starting burette or syringe volume refer to Burette Reading section under the Setup section for how to measure accurately 3 Place 25 mL of wine or must in the titration vessel We recommend using the 25 mL sampling pipette provided in the kit draw the sample up to the 0 mL mark and then dispense the sample SC 300 Pro Kit Manual 12 Version 2 1d into your titration vessel by letting the tip of the pipette touch the side of the vessel while the sample drains See Figure 17 NEVER pipette any reagents by mouth Also make sure the pipette you are using is completely clean before submerging into your wine sample Using the transfer pipettes see Figure 18 add about 2 mL Acid Reagent and 2 mL Reactant solution to the titration beaker the two transfer pipettes should come with red and yellow labels with red for the Acid Reagent and yellow for the React
30. n sometimes become dirty and poorly responsive l Immerse electrode tip in 0 1M HCI see above for how to make for about 15 secs rinse with distilled water then immerse in 0 1M NaOH you can use a little of your TA Titrant for this for another 15 sec Cycle the electrode through these solutions several times rinsing with DI water SC 300 Pro Kit Manual 25 Version 2 1d in between then rinse and check for performance in pH buffer 4 00 and 7 00 2 Some other tricks protein deposits can be removed by soaking in 1 pepsin in 0 1M HCI for 15 mins Inorganic deposits may be removed by soaking in 0 1M tetrasodium EDTA solution for 15 mins Grease and oil deposits may be removed by rinsing the electrode in mild detergent in methanol solution Instrument test You want to be sure that the instrument is responding correctly A quick test is to simply short out the electrode connector l Put the instrument in pH mode 2i Remove the electrode to expose the BNC connector at the back of the instrument Short out the terminals on the connector using a paper clip or similar metal piece to touch the center pin of the connector to its outer metal sheath 3 With the input shorted out the reading should be pH 7 00 0 50 If out of this range the meter is probably bad Contact us at info vinmetrica com or tel 760 494 0597 4 Bear in mind that this test is not 100 fool proof the instrument might still have trouble reading pH values diff
31. nmetrica Electrode Holder the Double Burette Clamp the Glass Burette and the Magnetic Stirrer Carefully pull out the glass burette from its cardboard cylindrical container and out of its container Use caution not to exert too much force on the burette as it is fragile and can break Open the Magnetic Stirrer from its packaging then insert the double AA batteries included For pH measurements or SO2 amp TA Titrations we recommend you replace the cylindrical container provided with the magnetic stirrer with the titration beaker Figure 4 included with the SC 300 Assemble the Lab Support Stand by attaching the long metal rod and screwing it into the large metal base We have found that adding a drop of super glue to the threads of the metal rod can prevent loosening of the assembly Then place the magnetic stirrer below the lab support stand as shown below in Figure 4 To attach the Vinmetrica Electrode Holder loosen the thumb screw on the Holder and slide it onto the Lab Support Stand rod Figure 5 then tighten the thumb screw to adjust it to an appropriate height Figure 6 Make sure the Vinmetrica logo can be read upright Attach the Double Burette clamp to the Lab Support Stand using its thumb screw to adjust for height as shown in Figure 7 below SC 300 Pro Kit Manual 6 Version 2 1d Figure 4 The Magnetic Figure 5 The Vinmetrica Figure 6 The thumb Figure 7 The Double stirrer and beaker with the Electrode Holder slides s
32. olution is being used and will display the apparent pH value This may be different by as much as 0 40 from the value of the reference solution e g the LCD may display 4 41 when the pH electrode is sitting in the pH 4 01 reference solution When the pH level is sensed as stable the nominal value is shown on the display flashing and the CAL LED flashes to convey that calibration for this value 1s ready Press the ENTER button to accept the calibration 5 The display stops flashing scrolling the message Good CAL and four beeps are rapidly sounded to indicate success Note if an error occurs during this process the message bAd SC 300 Pro Kit Manual 15 Version 2 1d CAL will scroll and a single beep will sound the instrument will then continue to wait for a stable pH level Repeat step 4 Following the Good CAL message the display will now show the calibrated pH value 6 Now rinse the electrode again and place it in the second member of the calibration set e g pH 7 00 reference solution Repeat the process to get a second Lood CAL message Exit into pH or TA mode 7 Force Calibrate feature firmware v3 0 6 and later 1f your instrument displays an apparently stable value that is within 0 5 pH value of the target 1 e 3 5 to 4 5 for pH 4 01 or 6 5 to 7 5 for pH 7 00 but the CAL LED does not flash within a reasonable amount of time 1 2 minutes at most typically you can force the instrumen
33. on l Disconnect any electrodes Turn on the instrument by pressing the POWER button briefly Note depressing the POWER button longer than two seconds at start up will cause the instrument to enter Test Mode see Appendix A The instrument will go through a power up sequence After a few seconds the instrument will start in whatever mode was last selected The mode is indicated by the yellow LED panel on the left Select the desired mode by pressing the MODE button 2 SO mode This is for titration in determining ppm of SO see below under Procedures The SO electrode must be used but do not attach the electrode yet Warning On older style single connector units pre 2014 the pH electrode can be damaged by connecting it to the instrument in SOz mode As a safety precaution you must press the ENTER button after selecting SO mode to confirm that the SO electrode is attached before the mode will be enabled However scrolling through the MODE selections such that you pass by the SO selection with a pH electrode attached is safe as long as you do not press ENTER to confirm the SO mode After pressing ENTER you should see the display show a value less than 50 usually 0 0 and the green PROCEED LED should be on Now you can attach the SO2 electrode 4 pH mode In this mode the meter measures the pH The pH electrode must be attached If the instrument has not yet been calibrated the message doCal scrolls across
34. on of the titrant and its volume required to reach the endpoint the free SO is simply calculated 2 pH and TA The SC 300 kit also provides a pH electrode and reagents for calibration and determination of pH and titratable acidity TA values in wines and other samples The pH value is simply determined by placing the calibrated pH electrode into a sample and reading the value TA is determined by titrating a 5 mL sample of wine to an endpoint pH of 8 2 with the TA titrant 0 133N NaOH from the syringe in the kit From the known concentration of the TA titrant and its volume required to reach the endpoint the TA is simply calculated results are in units of g L tartaric acid gt Potential measurements In versions 3 1 1 and higher of the firmware the SC 300 can display the voltage reading on an electrode attached to the pH connector This can be used with certain electrodes for example galvanic oxygen probes potassium or sodium electrodes or to view the raw voltage reading of a pH electrode SC 300 Pro Kit Manual 4 Version 2 1d Setup Setting up the SC 300 for the first time l The SC 300 See Figure 1 runs on two standard AA batteries alkaline cells recommended To insert the batteries open the battery housing on the bottom of the back of the unit by removing the two screws and gently prying off the lid Install the batteries with the negative end contacting the coiled springs and the positive end contacting the ra
35. rd or the back of a business card and draw a black band down the center of the paper about an inch and a half thick See Figure 12 When taking a measurement hold the paper about an inch behind the burette and the black band about a half an inch below the meniscus See Figure 14 This provides a clear view of the bottom of the meniscus which helps make a precise consistent measurement Second when filling the burette make sure the titrant in most cases this will be either the SO Titrant or the TA Titrant has completely filled the bottom of the burette including within the tip Sometimes bubbles can be trapped in the tip of the burette but can usually be dislodged by opening and closing the stopcock while the burette is hovering over a waste container We also recommend washing a couple of milliliters of the titrant you are using through the burette to remove any excess water or contaminants that may remain from a previous titration Finally make sure there are not any large bubbles in the burette after filling If there are cover the top of the burette with some saran wrap or parafilm if you have it and make sure the stopcock is in the closed position Then take the burette out of its clamp and hold the saran wrapped end tightly Rotate and invert the burette to allow the bubbles to move out of the column of titrant Once the bubbles have been displaced you are ready to titrate Note the gradations on your burette may be different than shown
36. rode is sturdy with its plastic housing but do take care not to let things touch or strike the SC 300 Pro Kit Manual 5 Version 2 1d platinum wires they are somewhat fragile and will break if bent and straightened repeatedly Electrode care When done always rinse with DI water and let air dry There is no need to store the SO electrode in any kind of solution pH electrode Attach the pH electrode via the BNC connector on the top of the SC 300 see Figures 2 amp 3 A note about the pH electrode The pH electrode is fragile and should always be handled carefully Remove the liquid storage bottle by unscrewing the cap first then gently pulling the bottle followed by the cap off the electrode Rinse the electrode in a little distilled water before each use Electrode care Do not touch the glass bulb nor attempt to wipe it with anything When necessary you may gently blot excess liquid away from the electrode surface but avoid directly touching it The pH electrode should always be kept in the liquid storage bottle with its pH storage solution available from Vinmetrica when not in use Rinse the electrode with DI water and gently blot or shake off excess water Push the electrode through the hole in the cap about an inch then gently screw the bottle onto the cap so that the electrode is in contact with the solution in the bottle Assembling the Pro Kit Equipment l Remove the items from their packaging the Lab Support Stand the Vi
37. s while turning the instrument on Remove the pH electrode if it is attached Test Mode is organized into sections Press the POWER button briefly to move to the next section After the last section Test Mode restarts the first WARNING DO NOT MOVE THROUGH SECTIONS WITH THE pH PROBE ATTACHED This can damage the electrode Only connect the pH electrode if needed in section 2 or 4 Always remove the pH electrode before leaving these sections Combinations of the yellow MODE LEDs are illuminated to indicate the section number as shown in the table below The Stop LED red is illuminated when an error is detected by the instrument The Proceed LED green is illuminated to indicate no error detected The green LED does not guarantee proper functioning it only indicates that no problem could be automatically detected The user should make careful observations to discern proper operation To exit Test Mode hold the POWER button down 5 10 seconds until the instrument shuts off If the device does not shut off after 10 seconds of holding down the button move to the next section by releasing then pressing again the POWER button briefly then try to exit again a ey 1 Version None The version number of the instrument software is displayed The instrument goes Mounn a 2 Burn in None continuous burn in cycle exercising relay sound LEDs and display 3 pH probe or precision An uncalibrated pH level is shown in two
38. suring Free SQ by Titration 1 With the electrode disconnected turn on the instrument and select SO mode with the MODE button The display will scroll across reading PrE55 EntEr Press the ENTER button to confirm selection of SO2 mode Figure 15 The display should show a value less than 20 usually 0 0 Now attach the SO2 electrode Figure 15 Once in SO gt Figure 16 Withdraw Figure 17 Dispense 25 Figure 18 The transfer MODE the instrument the titrant from its bottle mL of your wine into the pipette One full squeeze should read using a clean 5 mL titration beaker using the of the transfer pipette in Press Enter Press syringe If you are using 25 mL sampling pipette either the Acid solution ENTER to activate SO the glass burette use the Make sure this is clean or Reactant should be MODE syringe to fill it before putting the pipette approximately 2 mL into your wine container pA Fill the syringe by drawing up the SO Titrant the bottle with the blue label Figure 16 Expel bubbles and set the plunger on the syringe to a readable point preferably the 5 0 mL point Make sure the outside of the syringe is dry to minimize any inaccuracies Note the 5 0 mL setting allows determination of up to 100 ppm SO in a standard 25 mL wine sample If using the burette use the syringe to dispense the SO titrant into the top of the burette Make sure the burette stopcock is in the closed position where the red handle is hor
39. t If your TA is too high before bottling you can try cold stabilization This results in precipitation of potassium acid tartrate potassium bitartrate to decrease the tartness Another method to decrease your TA level is to add calcium carbonate or potassium carbonate CaCO or K2CQO3 For the chemically inclined we recommend Zoecklein s book Wine Analysis and Production which goes over theory and practice behind these adjustment techniques and many wine analytical techniques Appendix C1 Troubleshooting pH and TA Issues I can t calibrate the pH on my SC 300 When calibrating your pH electrode remember these points l The displayed pH may not be correct until after you press ENTER 2 If the instrument signals stable pH but displays Bad Cal after pressing ENTER try laying it flat on the table when the next stable signal is signaled press the ENTER button quickly without handling the instrument Sometimes the instrument may pick up noise from its environment particularly if you handle it at the last second while it s trying to achieve a stable reading This sensitivity is usually only an issue during calibration 3 If values appear to drift leave the electrode in the pH 4 01 reference solution for 30 minutes 4 If you intend to read pH values in samples that are at a different temperature than ambient it s best to have your reference solutions at that temperature also before calibrating S If the displayed pH
40. t to accept the calibration by following these steps a Use the MODE button to select TA mode b Press and hold the MODE until the display goes from LAL to ce AL i e the C will change to lower case c c The display will then show PHY Place your pH electrode in the pH 4 01 reference solution and press ENTER d The apparent pH will be displayed Allow it to come to a stable reading then press ENTER If the reading is within 0 5 pH unit as above the Lood CAL message will scroll and the four beeps will sound as usual Otherwise the message bAd CAL will scroll and a single beep will sound In that case you can return after the next step and try PH4 again e The display will then show PH Place your pH electrode in the pH 7 00 reference solution and press ENTER then repeat the procedure in step d Measuring pH l Make sure the pH electrode is attached Calibrate it as described above if necessary Select pH mode with the MODE button 2 Rinse the pH electrode with DI water Gently shake off or carefully blot away excess liquid 3 Place the electrode in the solution to be tested Be careful not to let the electrode strike any surfaces 4 Allow the pH reading to stabilize Typically this takes about 10 15 seconds Read the pH value on the display Note Once you ve completed the pH test you can start the TA test Start SC 300 Pro Kit Manual 16 Version 2 1d with precisely a 5 mL wine sample
41. te swirling motion If the electrode is not held in a stand hold it against the side of the vessel with one finger and grasp the vessel with the remaining fingers so that the two move together See Figure 19 Verify that the current is less than 50 and the green PROCEED LED is lit See Figure 20 If the current is greater than this and or the red STOP LED is lit and the buzzer sounds your sample has less than 2 ppm SO and there is no need to proceed Titrate the sample by adding the SO Titrant drop wise from the syringe See Figure 19 or from the burette See Figure 20 being sure to note the starting volume mark on the syringe or burette Try to accomplish the titration as rapidly as possible in 3 minutes or less but be careful near the endpoint so as not to overrun it here dispense one or two drops at a time Be sure to maintain stirring or swirling throughout the entire procedure If the magnetic stirrer turns off turn it back on SC 300 Pro Kit Manual 13 Version 2 1d Figure 19 Manual stirring Figure 20 Automated stirring Figure 21 Make sure Figure 22 Once the device technique Hold the electrode technique Turn on the that the PROCEED beeps for 15 seconds or 20 against the side of the titration magnetic stirrer add SO2 LED is lit You should be sets of beep beep beaker and swirl gently add Titrant by slowly opening the reading close to 0 0 when you are done with the SO2 Titrant with other hand bur
42. the screen and you need to do a calibration see Calibration of pH below We recommend re calibrating the instrument for pH once each day of use Warning Be careful not to press ENTER when the pH electrode is attached while the instrument is in SO mode as this can potentially damage the pH electrode If this does happen and the pH electrode does not respond correctly place it into the pH 4 Reference solution for an hour and it should return to normal function 5 Potential mode Firmware version 3 1 1 and higher pH LED flashing In this mode the instrument displays the potential in volts or millivolts in version 3 1 2 and above coming from an electrode attached to the pH connector This can be used with certain electrodes for example galvanic oxygen probes ORP potassium or sodium electrodes or to view the raw voltage reading of a pH electrode In this mode when the red STOP LED is illuminated the values on the screen are negative otherwise the values are positive SC 300 Pro Kit Manual 10 Version 2 1d TA mode This is for titration in determining titratable acidity TA As in the pH mode the pH is displayed and the pH electrode must be attached The green PROCEED LED is lit if the pH is below the TA endpoint normally 8 2 but can be set to 7 0 see Appendix A Test Mode section 16 while the red STOP LED 1s lit if the pH is above the endpoint see below under Measuring TA by Titration CAL mode This is for cali
43. titrant Take the endpoint as the first addition of TA Titrant that causes the pH to stay above the TA endpoint 8 2 or 7 0 depending on your setup see Appendix A Test Mode section 16 for longer than 15 seconds The red STOP LED and the beeper will provide additional indication of the endpoint See Figure 26 Read the endpoint volume off of the syringe or burette To silence the beeper after the endpoint select pH mode or turn off the instrument 10 Calculate the TA value as V x 0 133 x 75 TA g LTartaric 7 SC 300 Pro Kit Manual 18 Version 2 1d where V mL Titrant needed to reach the endpoint 0 133 normality of the Titrant mL sample If you use 5 mL of sample as directed and the Titrant is 0 133 N as supplied then the calculation is simply TA 2x V i e 2 times V Note to express these values as tartaric acid divide by ten e g if the TA is 7 1 g L that is equivalent to 0 71 tartaric acid Finishing up l Turn off the instrument 2 Rinse the SO electrode and syringe with distilled water Let air dry gt Be sure to rinse the pH electrode with distilled water and then store it in its storage solution as directed under Setting up the SC 300 for the first time item 6 page 6 of this manual 4 Store all reagents tightly capped and away from heat and sunlight 9 Discard waste samples and solutions in accordance with local regulations Acidic solutions can be neutralized by slow addition
44. troduce these spilled titrant droplets into the wine sample leading to an inaccurate reading Be sure to record your starting burette or syringe volume Caution the TA Titrant is caustic and can cause damage to clothing skin and eyes We recommend use of laboratory safety glasses and latex or nitrile gloves during this procedure If any solutions contact skin or eyes flush with plenty of water 3 Place 5 0 mL wine or must in the titration vessel 100 mL polypropylene beaker We recommend using the 5 mL pipette provided in the kit draw sample up to the O mL mark then dispense the sample into your titration vessel by letting the tip of the pipette touch the side of the vessel while the sample drains For best accuracy do not blow out the liquid that remains in the tip Add about 15 ml of deionized DI water distilled water 4 Turn on the instrument Make sure the pH electrode is attached If necessary calibrate it as described above Select TA mode with the MODE button 5 If you are using a magnetic stirrer turn it on to stir at a moderate rate Be sure the stir bar will not strike the electrode in the following steps See Figure 24 6 Rinse the electrode briefly with DI water Insert the electrode into the titration vessel so that the tip is fully submerged to just above the circulation gaps cutouts at the tip of the electrode SC 300 Pro Kit Manual 17 Version 2 1d 7 If you are stirring manually begin now use a moderate swirl
45. w the Amount of sulfite to be added and look below to the Notes section You should now see the message 1 The recommended level of free SO for this type of wine molecular SO2 amp pH is your value mg L 8 Go back to step 4 and enter this value for the Desired level of free SO 9 Press Calculate again to get the correct amount of sulfite to add We recommend double checking your calculations Also be sure you are using fresh KMBS Once you have added the recommended amount of sulfite stir your wine thoroughly and take another SO measurement after waiting at least 30 minutes If the measurement matches the Desired level of free SO then you are done otherwise make incremental additions and repeated SO2 measurements until you reach your desired level Adjusting TA in your wine We recommend adjusting your titratable acidity levels by adding tartaric acid for non grape wines fruit acids can be added but use caution malic acid if dosed improperly can make wine overly SC 300 Pro Kit Manual 23 Version 2 1d sour or tart If your wine s pH is too high indicating low acidity and TA levels too low you can add tartaric acid to decrease the pH and increase TA By measuring TA you can figure out how much tartaric acid to add without making your wine overly tart or sharp As a rough rule of thumb adding 1 g of tartaric acid per liter of wine will increase the TA by 1 g L 0 1 and reduce the pH by 0 1 pH uni
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