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QCapture PRO 7 User Manual

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1. Reset to Default OK Cancel Reset The Q Capture Pro 7 Application Window File Options let you decide how to save and load images and ROIs General options ask you to specify a file name for new documents Image Saving options allow you to save your ROIs and annotations with your image Image Loading options let you turn the Best Fit display on or off automatically when you open an image Quick Save for Publication and Quick Save for Analysis allow you to specify the file path and format of any images that you want to save to work with at another time Q Capture Pro 7 Options x Popular z ted File Image Application General es si Prompt for filename for new documents Paste Histogram amp Profile Image Saving Status Bar Save annotations Capture Z Save ROIs Display Z Crop to ROI in Save As Image Loading Z Best Fit Display Quick Save for Publication Folder C Documents and Settings All Users Documents Q Capt Filename preft Picture Filetype JPG Output image size 0 original size poes Z Auto open Quick Save for Analysis Folda C Documents and Settings All Users Documents Q Capt Les Filename prefx Image Filetype TIF gt OK i Cancel Peset Quick Save for Publication and Quick Save for Analysis allow you to specify the file path and format of any images that you want to save to work with at another time
2. 14 Qlmaging maging PVCAM Capture Interfac Destination Folder s Where would you like Qlmaging PVCAM Capture Interface to be installed Install Qimaging PVCAM Capture Interface to Image Pro Insight 8 0 Install to C Program Files amp 86 Media Cybemetics Image Pro Insight Image Pro Premier 9 1 Install to C Program Files amp 86 Media Cybemetics Image Pro Premier 9 15 QCapture Pro 7 0 Install to C Program Files amp 86 GQImaging G Capture Pro 7 Indicate the program location then click the Next button Installation The Ready to Install dialog box appears 3 Qimaging PVCAM Capture I key magin Ready to install Qimaging PVCAM Capture Interface Click Install to begin the installation Click Back to review or change any of your installation settings Click Cancel to exit PVCAM version 2 9 11 or later is required to ensure corect operation This screen indicates that your computer is ready to install the PVCAM capture interface 6 Click Next to continue Please wait while Qimaging PVCAM Capture Interface is installed Status 7 The setup program will begin copying and installing files Click Next when the installation is complete 1 18 Installation The next page shows that the capture interface installation is complete Completed the Qimaging PVCAM Capture Interface installation Click the Finish button to exit Click the Finish button
3. Aspect ratio refers to the ratio of vertical to horizontal lengths For example when a television screen s image appears flattened or squeezed the ratio of horizontal length to vertical length is out of proportion Similarly cameras often inadvertantly skew images in the process of capturing them and translating them to digital format Such skewing results in inaccurate lengths being ascribed to features that are measured in the image When a skewed aspect ratio has a flattening effect vertical lengths will be recorded as shorter than they actually are when it has a squeezing effect horizontal lengths will be recorded as shorter than they actually are If an image has a known aspect ratio problem Q Capture Pro 7 can compensate for the flattening or squeezing of vertical and horizontal lengths and calculate the actual number of units that measurements of its features represent To do this you must be able to supply it with the value h length divided by height for a perfect square as represented in the image or there must be a feature pictured in the image that 3 13 The Capture Tab represents a perfect square If a square feature is pictured Q Capture Pro 7 can calculate the aspect ratio from that feature Companies often plant a perfectly square object into images for this purpose Creating a Spatial Calibration To create a new spatial calibration you must either know the number of pixels per unit to specify fo
4. Rank 1 Click the radio button next to one of the filters 2 Click Preview to see how it will look on a sample portion of your image You may try this with different filters to see the various effects The sample area appears in the center of the image 5 33 Image Sequences CASTERS TF b Sek 2 i E E E i P lt hy i s q Zz es cr a h be b JLL 4 HoF s ray om 4 i a ha a Iae 5 i Y Click Apply to apply the filter to the whole image Image Sequences Q Capture Pro 7 allows you to open and navigate multi frame images saved in the seq format The sequence viewer is automatically used for images that have more than one frame The sequence viewer has an on screen toolbar which appears only when you move your mouse over the image The example below shows the sequence toolbar at the bottom of the image area as window decoration The toolbar has the following buttons Go to first frame Go back one frame E Play sequence backward Play sequence forward g Go forward one frame D Go to last frame 5 34 Image Sequences sy Use active range applies filters and other operations to the active range of ae on or to the individual active frame only off The i options button opens the following options dialog Set Start 1 Set End 10 Fate 15 00 Loop gt WW Tap Click Set Start or Set End to set the current frame as start or end of
5. 2 10 The Q Capture Pro 7 Application Window Paste Options allow you to control the pasting blending and masking of data and images Action Options let you preview blending and pasting on an image ROI After Paste features a drop down menu of choices for the source and destination ROIs Blend Control lets you specify the degree of blending and choose to apply blending to lighter darker or all pixels Action Masking lets you decide how to apply masking to an ROI 2 11 The Q Capture Pro 7 Application Window Histogram amp Profile control the display of an image histogram or line profile General Options control what type of histogram is displayed View Options determine what information is displayed in the histogram legend Additional Options let you decide what areas of the image will be included in the histogram Line Profile Options determine what the initial display of the line profile will show Q Capture Pro 7 Options x Popul ee ln Histogram amp Profile General 4 Histogram D Dynamic LUTS A BW Levels L Ignore ROIs A Active Frame Mone Interpretation Exclude Bins A Auto Open Close ag Line Profile Initial Profle Default An Image Histogram can be displayed in the workspace by going to the Capture ribbon and selecting Image Histogram from the Intensity group A sample histogram is shown here Additional information
6. 3 24 The Capture Tab Calibration Marker ie libration Marker ME HER Add HS Remove BG Aue Arial fe f Text Size so hE Marker Size JSU E Shadow un E position and sizeof calibration marker can be Ms al adjusted on image Use the Position buttons to place the marker on the image You can resize the marker and the length and the label will be adjusted automatically based on the current size The length is rounded to the nearest value from the list of 10 logarithmically equidistant values 1 1 2 1 5 2 2 5 3 4 Set the Font and Text Size options the way you want them To hide the text and only display the marker set the font size to zero Use the Size spin box to indicate the size in points to be used for the text Use the Color button to change the text color 3 25 The Capture Tab 4 Use the Shadow drop down list to set the background Imm No background M Filled background i irreg Chad Use the Color button to change the color of the background Select No background if you want to suppress the background TIP You may need to experiment with the Text and Marker settings to get the desired effect Go with your best guess the first time you set the marker Once the marker is added to the image you can easily delete it and define a new one see Deleting an Annotation in Chapter 7 Annotating Images
7. Setting the Appearance of Annotations later in this chapter Arranging Annotations After you have created a set of annotations you can move them resize them layer them and delete them Moving an Annotation To move an annotation 1 Click the Selection tool Le from the Annotate group 2 Click on the annotation you want to move The annotation is selected 3 Position the cursor over the selected annotation If the cursor is positioned correctly the Dragging tool ahh appears 4 Click and drag the annotation to the desired location TIP You can also move the annotation by pressing the lt Shift gt key along with the arrow keys up down right left For example press the lt Shift gt and lt Up Arrow gt keys and notice the annotation moving upwards Rotating an Annotation Annotations can be rotated by using the Select tool and the lt Control gt key 1 Click the Selection tool he from the Annotate group 2 Click on the annotation you want to rotate The annotation is selected 3 While the Annotation is selected move your cursor to the center point of the Annotation The rotate cursor a double ended arrow will appear enabling you 6 7 Arranging Annotations 6 8 to rotate the annotation either clockwise or counter clockwise Holding down the lt Shift gt key while rotating an annotation will move the annotation 1 15 degree increments You can also rotate an annotation while creating o
8. You may see the following message Oo Warning PVCAM Required Before you can use your camera you must install the PVCAM This installer can be found on the installation disk provided with your camera and can also be downloaded from the QIlmaging PVCAM support Web site If you have not yet installed these files you must do this before you will be able to use your camera Would you like to visit the Qimaging PVCAM Web site now Click Yes to go to the Qlmaging web page http www QImaging com support downloads From here you will be able to select and install the correct Q maging support software for your Q maging camera 1 19 Installation Updating Your Q Capture Pro 7 Product Q Capture Pro 7 runs on the following operating systems e Windows XP SP 3 32 bit only e Windows 7 Professional and Ultimate SP 1 32 and 64 bit Software Registration and Updates Updates for Q Capture Pro 7 through version 7 0 5 are available for users running older versions of the software You do not need to re register your product if you have previously done so You can install and use version 7 0 4 of the product without registering but you will not receive any future updates The version 7 0 4 release of Q Capture Pro contains some changes to the licensing and updating processes e New Q Capture Pro 7 users can start using the software immediately If you are a new user you will receive a pop up message asking you to registe
9. rotating 6 7 setting the appearance of 6 10 Apect Ratio 3 19 Appearance Annotations 6 12 ROIs 7 12 Application button 2 4 Application Options 1 23 2 9 Application Tools 1 23 2 6 Application window components 2 2 Applying enhancements to an image 5 29 Arranging annotations 6 7 Aspect Ratio 3 13 3 16 3 19 Auto Trace 4 9 Assume clockwise 4 10 Auto accept 4 10 Auto start 4 10 Disable 4 11 Index 1 Index Edge quality 4 10 Momentum 4 10 Monochrome interpretation 4 10 Noise reduction 4 10 Range 4 10 Remove redundant points 4 10 ROI bound 4 11 Smoothing 4 10 Tracing speed 4 9 Trim leading points 4 10 Auto Trace tool for defining ROIs 7 4 Auto White Balance 3 8 Auto Range 4 2 Auto Trace tool 6 5 AVI file formats 10 1 B Background Correction 5 30 Background Correction Command 5 30 Background From Points 5 30 5 31 Background Subtraction 5 30 Background Tools 4 8 Best Fit 4 2 5 27 BMP file formats 10 1 Bright Background 5 30 Brightness 5 27 Brightness adjusting 5 27 Burn In 6 5 C Calibrate by feature 3 14 3 20 Calibration Marker 3 24 Calibrations applying a calibration marker to the image 3 24 creating a new calibration definition 3 14 described 3 13 saving 3 22 Camera group 3 2 3 3 3 4 Canvas 5 17 Capture controls 3 5 Index 2 Capture Enhance Controls 3 6 Capture Options 2 14 Capture tab 2 4 2 18 2 20 3 2 4 2 4 4 Capturing
10. Green e i E 100 4 Bue 9 m w Auto White Balance This option is available only when the camera is capable of capturing a color image Each color channel may be changed individually using the slider or the numeric spin buttons The Auto White Balance button will start a live preview if one is not already active and starts the process of calculating new color ratios in order to establish a white balance based on the input image 7 The Custom Controls contains camera manufacturer specific controls The example shown below is for a Q maging camera Readout Speed 20 Mhz Ea Sync B out Expose E Bayer Mode Bilinear interp olation Preview Color Corredion Acquire Color Correction You will need to consult your camera documentation to learn more about options for these settings 8 The Naming Options let you assign a name to your captured image Auto name captured images Prefix Acquire 4 Format Date and Time expanded Number Suffix Start at Z lak Mindigits W Sample Acquire 2010 03 12 02 10 56 002 Check the Auto name captured images box if you want the application to automatically generate a name for each captured image There are a number of 3 8 10 The Capture Tab formats available The prefix such as Acquire lets you identify an image or series of images Number suffix would be 01 02 etc and you c
11. User s Guide This version of Q Capture Pro 7 is designed to run under the Microsoft Windows 32 bit or 64 bit systems Windows XP Professional service pack 3 or Windows 7 Professional or Ultimate service pack 1 Product Features Q Capture Pro 7 is anew application for basic image acquisition and advanced measurement It provides a set of basic acquisition and measurement functions designed to simplify routine image capture inspection and measurement tasks This software makes it easy to preview acquire annotate and perform basic analysis functions on an image or image sequence With Q Capture Pro 7 you can m Acquire images quickly and easily Read and write image data in many image file formats m Use a customizable user interface so that you can set up Q Capture Pro 7 in the most efficient way for your work including dockable tool bars and the ability to show or hide different toolbars and buttons You can also reset your interface to factory or default settings if you don t like what you ve set up and want to Start Over m Measure image features to ensure that they conform to defined standards m Use the data viewer tools to view and analyze your data m Work with gray scale data in 8 12 16 or 32 bit floating point depths Work with color data in 24 36 or 48 bit format m Make features of interest in images more defined by manipulating the settings for gamma contrast
12. e 0p 9 r g te e P a Li he ig amp Seat e e 5 p oe s e s a Fas 9 kd e a bon tree nea K2 Click and drag the center of the viewing area on the thumbnail to control pan and scroll of the image The Image Navigator feature allows you to use your mouse to move the white rectangle around in the image strip The zoomed portion of the image in the workspace will move as you move the thumbnail in the image strip Enhancing an Image Enhancing an Image There are several tools available through Q Capture Pro 7 s Adjust tab for visually enhancing objects of interest in your images Q Pady V seu tees Toe gt Capture Pro 7 NUCSTAIN TIF Cauliwe Adjust Select Meme View imege 2 aa Mone tige Ar i a TA oao aj 94 2 Baich STE TEE J Le Se 2_ DF SS id 3 F corea TSubsamige Revue Rotate Camas S APP 7 bbad Tom pe we Ee 12 tg eet F gr sc am ye ne Com nre Trarnkone Carevert Coke Display Rackyrocre This section provides instructions for using some of them Colors Filters Background correction Image transformaton Resizing an Image Image transformation allows you to rotate or resize the image in the Q Capture Pro 7 workspace These options allow you to examine the image in more detail To resize an image follow these instructions 1 Open the castiron tif image in the Q Capture Pro 7 workspace as described earlier in this chapter 2 Select the T
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14. ficas 7 Apply i Shape Class 4 Click on the points in your image to tag them with a color and label o Class 1 2 Class e Class to 1 Class 1 Class 1 e Information about your tagged points appears in the Data Table below your image Data Table Intensitylum 8 6 Manual Tagging 5 Save your data to a file or export it to Excel ax Sendito Erea z Save to File Setting the Default Appearance for Measurement Labels The default appearance attributes for measurement labels are set by designating the appearance attributes of an existing label as the default for future measurement labels To set the default appearance for measurement labels 1 Click Select pe from the Measurements group 2 In the active image click on a label of the type for which you want to set appearance defaults Most labels appear at the top left or bottom right positions near the marker 3 Click the Options r button You will see the Meaurement Options panel Measurements Options hx poprimi pe Text Name And Meas Position Center wv M4 Units Abbreviation Decimal Points 2 S Cross Bars Crosshair 4 Tooltip Manual Tagging 8 8 4 Go to the Font panel Arial ji Don EE Choose a font from the drop down list Aria Arial Arial Black Arial Narrow Baskerville Old Fac Bauhaus 93 Bell MT Berlin Sans F
15. mouse over an object on the image Q Capture Pro 7 automatically saves any changes you make and applies them to all future labels 8 9 Viewing Measurement Data Viewing Measurement Data The Measure ribbon contains the data table feature to collect and view measurement data This feature is described below Viewing a Data Table The Data Table collects all the information from the measurements taken on your image To view a data table 1 2 8 10 Open the image if it is not already open Select the points or lines that you want to measure Click the Data button in the Measurements group The data table will appear in a pane below your image Data Table a x zm E 1 ba Aix Vv Feature Name Class Name Location Xipin Location Yipiki Intensity iliim Tl Class 1 126 20 135 91 213 86 Class 1 325 23 466 98 177 47 Class 1 258 24 169 89 199 83 Class 1 501 93 164 06 206 07 Class 1 257 27 107 75 190 38 Viewing Measurement Data The Data Table provides the following tools PBE AY Load Data Table Measurements This tool allows you to load measurements saved in a previous sesssion from a ipm file Save Data Table Measurements This tool allows you to save your measurements ina ipm file for use at another time Export to Excel This tool allows you to save your measurement data to a file or export it to an Excel spreadsheet Show all Statis
16. this group allows you to add a new lens from the Predictive Calibration dialog The Auto Calibration and Zoom features are no longer available The Optical Characteristics group now displays only the lens selection tool The calibration drop down list has been removed from the Characteristics and Calibration groups System Calibration has been removed from the user interface All new calibrations will automatically be Reference calibrations Captured images are displayed behind the preview images The time required to display captured images has been improved A problem in Preview Capture and Locked where they sometimes need to be changed multiple times before they work has been fixed When locked display range adjustments on preview images can be applied to captured images When locked gamma adjustments on preview images can be applied to captured images A problem where the bit depth used while capturing an image is not the same after the image has been saved is fixed An application freeze while moving the white balance sliders has been fixed A problem with captured timelapse images in the Movie feature has been resolved Installation Installing the 7 0 5 Update If you are running an unregistered copy of the Q Capture Pro 7 version 7 0 4 software you will see this message indicating that an update is available Q Capture Pro 7 Update Update Available Press Next to Download and Apply this Update to Q Captur
17. AMD Athlon 64 processor 2 GB of RAM 500 MB of available hard disk space DVD CD ROM drive Microsoft NET 3 5 Framework automatically installed by application USB port required for hard licenses and offline license activation Internet connection required for online services Recommended Requirements Dual or multi core processor 4 GB of RAM or more 20 GB or more of available hard disk space Suggested storage size if acquiring large image files DVD CD ROM drive Microsoft NET 3 5 Framework automatically installed by application USB port required for hard licenses and offline license activation Internet connection required for online services 1 2 Installation Installing Q Capture Pro 7 To install Q Capture Pro 7 follow the steps below Note that Q Capture Pro 7 will not install on versions of Windows older than Windows XP service pack 3 1 Insert the Q Capture Pro 7 product CD into the CD ROM drive If the program does not start automatically you can install Q Capture Pro 7 using the Start button on your desktop Choose Run and then use the Browse button to find the autorun exe file on the CD Double click autorun exe to begin the installation process You will see the Q Capture Pro 7 Start screen Q CAPTURE Pe07 gt ata Wp E 2 Click Install Q Capture Pro to begin 1 3 Installation Please wait while Windows looks for the setup program You will see the Welcom
18. By refining the Text and Marker settings on subsequent iterations you can achieve the desired effect Click OK The calibration marker is added to the image When calibration markers are initially added to images they are added as annotation overlays This means that they can be moved and deleted the same as any annotation This also means that they will only be saved with the image 1f that image is saved in a TIFF format 3 26 The Capture Tab Intensity Features The Image Histogram Line Profile and Saturation features of Q Capture Pro 7 appear in the ntensity group on the Capture ribbon aml Profle 2 oil Intensity Image histograms measure and illustrate in graph form the brightness and contrast characteristics of an image Histogram data can be created and viewed for data gathering and analytical purposes or can be manipulated for image enhancement purposes Line profile analysis allows you to collect the actual index values along a line that you define A profile plot shows the pixel positions of the line along the X axis and on the Y axis measures the index value for each position along the line In a line profile the X axis represents the spatial scale as defined by the spatial calibration and the Y axis measures the intensity value You can display a line profile on both live and captured images Use Saturation to show overexposed and underexposed areas with a given color It can be used with the live pre
19. Calibrations in Chapter 3 for more information Draw a line on the image The measurement information will appear in a label on the image The label will appear near the center of the line without covering up the end symbol or much of the line itself Making Manual Measurements You can make changes to the appearance of your measurements using the Class group on the Measure tab s ribbon 7 TE Shape P z ial T Class This feature allows you to classify different measurements and classes by assigning them different colors You may also change the class name Use the Shape and Color buttons to assign properties to each class or group of measurements Your changes are automatically applied to the measurements on the image You can export the data to an Excel spreadsheet or other program if you want Note Measurements and Manual Tagging can be applied to live preview images for quick measurement previews However these measurements are not saved with the captured image 8 5 Manual Tagging Manual Tagging Use the Manual Tag tool to select individual objects or groups of objects in an image with your mouse You can create different classes of objects for analysis and tag each object with a specific marker To use Manual Tagging 1 Open your image if it is not already open 2 Select the Manual Tag tool from the Measurements group 3 Select a class name shape and color from the Class group
20. Click Next to continue Notice the icons appearing next to the names of the various products They illustrate three possible situations Already Installed If the current version of the interface is already installed you will see the information icon and this message ifj A ed mi m ap Already Installec ats C Program Files 86 Media Cybemetics Image Pro Premier oO Version 9 0 65 9 The same one this Setup installs of the capture interface is already installed This item will be repaired f needed A complete uninstall from all targets is required to remove this Please contact technical support if you have questions Older Version Installed If the application detects an older version of the interface you will see the question mark icon and this message EP installed product version 9 0 65 9 The installed product is newer than the product in the setup Setup product version 9 0 64 8 Would you like to run the install Click Yes to continue with the install or No to cancel the installation 1 10 gt 9 Installation Downgrade Not Permitted If the application detects a newer version already exists on your system you will see this message Downgrade of Qlmaging Capture Interface 9 Not Permitted This setup QIimaging Capture Interface 9 9 0 64 8 does not support a downgrade of Qlmaging Capture Interface 9 9 0 65 9 on your computer Click Cancel to exit In any of these cases cl
21. Contrast are implemented through a Lookup Table the changes made to the appearance of your images are not permanent This means they will not be saved with the image To make the enhancements permanent Click the Apply Display to Image v button All Lookup Table values are permanently applied to the image and the Lookup Table is reset to its default state 5 29 Enhancing an Image Correcting the Background Use the Background Correction command to make adjustments to the background of your image This tool is used to better distinguish image background from image objects making it easier to extract the objects during a counting or measurement operation The Background Correction command can correct uneven background intensities and compensate for irregularities due to uneven lighting nonuniform camera response or minor optic imperfections It might be used to remove evidence of dust on the lens or to correct for bright spots caused by the light beneath the microscope s stage Q Capture Pro 7 provides several tools that allow you to adjust the background of your image They appear in the Background group on the Adjust ribbon er Subtract See Correct al Options Background Background Subtraction should be used for all images except those of transmitted light experiments which will be used to measure optical density You have the following choices Dark Background Choose this setting to create a background ima
22. Olmaging Fira viro or USE 20 Camera ch iches yi ling ayah Cowilead legacy aoee or pee moth ditaded niana nequiding ihe await E Shwe Downloads jage Spbu E Sotware Raama Motes B Product Reaal E Recommanded Comeutars E Wonanty Plas E Crrtact lt GVE STISHEI Bea Volos Product Kon 20 H 3 Rewer Modes be Pabefa Thuria ded Go Ciera are eal suppeded Bundle and Sawe Neona mabumarig able suppl ik Buy with Confidonce http www QImaging com support downloads an tha icpn that From here you will be able to select and install the correct Q maging support software for your Q maging camera Installation Installing the Qimaging PVCAM Capture Interface To acquire images with your imaging software you must first install the appropriate capture interface The capture interface installation will begin automatically after the Q Capture Pro 7 software installation is complete These instructions cover the installation of the QImaging PVCAM capture interface only You should also install the PVCAM driver from Photometrics The PVCAM driver is available on the CD ROM provided with your camera and can be downloaded from the Photometrics website at http www photometrics com support downloads Recent improvements to the PVCAM driver may affect compatibility with older software products If your system is already configured to use your camera in another software application please confirm compatibility with your software
23. Pseudo Coloring an Image Use the Pseudo Color command to colorize an active monochrome Gray Scale 8 Gray Scale 12 Gray Scale 16 or Floating Point image You might do this to highlight certain features in a gray scale image For example you might want to display all densities above a certain point in red or if your imaging device recorded thermal information all temperatures below a certain point in blue You might also use it to visually amplify specific intensities that are very difficult to distinguish from their surroundings For example features produced by pixels with values of 122 in a field of pixels with similar values would be impossible to see in a gray tone image but would jump out if that value were to be rendered in color 5 22 Enhancing an Image When you pseudo color a monochrome image you build a special palette through which your monochrome image is displayed Pseudo coloring an image does not modify the pixel values in your image bitmap in any way it does not convert your image to True Color or Palette for example It simply associates a pseudo color palette with the image that interprets the gray level values in the image as color Pseudo colored images are very similar in structure to Palette class images but they differ in a couple of important ways First the pixel values in a pseudo colored image actually represent continuous tone intensity information whereas a Palette image s pixels carries no i
24. Q Capture Pro 7 Ribbon 2 4 Q Imaging Capture Interface Installing 1 8 Quick Access Toolbar 2 16 9 2 9 3 9 4 9 5 Adding commands to 9 4 Quick Access Toolbar using 9 2 Quick Calibration Method 3 14 Quick Save 3 11 5 7 5 37 Quick Save as Movie 3 11 Quick Save for Analysis 2 10 3 11 5 7 Quick Save for Publication 2 10 3 11 5 7 R Recent documents 2 1 5 1 Rectangle by Coordinates tool for defining ROIs 7 4 Rectangle tool 4 7 6 4 Rectangle tool for defining ROIs 7 3 Index Region of Interest See ROIs Regions of Interest 3 7 7 1 Requirements hardware and software 1 2 Reset Display button 5 29 Resetting images 5 29 Resize 5 13 Resizing an annotation 6 8 an ROI 7 6 Resizing an Image 5 13 ROI Display Options 7 5 ROI group 7 9 7 12 ROIs defining 7 3 deleting 7 8 described 7 2 moving 7 6 placing more than one ROI in an image 7 6 resizing 7 6 rotating 7 8 setting the appearance of 7 9 tools described 7 3 Rotate 5 15 Rotate tools 5 15 5 16 Rotating an annotation 6 7 an Image 5 15 an ROI 7 8 S Saturation 3 27 4 1 Saturation button 4 11 Saturation Warning 4 11 Save as Movie 5 7 5 37 Save Calibration dialog box 3 23 Save Measurements 8 11 Save to File Options 3 9 Saving calibrations 3 22 Saving an Image 5 6 Saving Annotations 6 14 Scale Bar 5 10 Select tab 2 19 6 2 6 3 Index 5 Index Select tool 6 7 Select Tool 4 6 Selection tool
25. Q Capture Pro 7 to receive the update 8 You will see this message Q Capture Pro 7 Update Update Available Press Next to Download and Apply this Update to Q Capture Pro 7 There is a newer version of Q Capture Pro available version 7 0 5 Build 4290 This update adds features and resolves issues to streamline workflow and facilitate operations with Q Capture Pro 7 4 complete list of changes is available by pressing the Details button below Please press Next to install this update now or press Cancel to leave the existing version in place and no change will be made Cancel Note that if your software is NOT registered or you are trying to run TrueUpdate from the Windows Start menu the Next button will be disabled 9 Click Next to install the update or Cancel to stay with version 7 0 4 1 32 Installation Q Capture Pro 7 will begin downloading the new software gt Q Capture Pro 7 Update Downloading File Please wait while the file is downloaded Downloading G Capture Pro 7 0 5 exe Estimated time left 00 00 Transfer rate 0 00 KB Sec The next dialog asks if you want to install the software update Q Capture Pro 7 0 5 update 1 This package will install the Q Capture Pro 7 0 5 update Do you want to n continue C 10 Click Yes to continue After downloading the file Q Capture Pro 7 will extract the updated file The next dialog shows that the update is bei
26. Save As dialog Saen Ejimaes S H e 0a m align amp EDF ED Background My Recent Calibrate Documents color E Filter Line Profile Desktop Measure My Documents My Computer on File name Snapshot_of_Paper_seq v Save as type Audio Visual Interleaved AV I avi Cancel My Network Places Q Capture Pro 7 takes a snapshot of your image sequence 3 Click Save Your snapshot is saved as an AVI file 5 37 Image Sequences 5 38 Chapter 6 Using Annotations Annotations are used to add text or drawings to your images The annotations are in a graphics overlay that is displayed over the image without modifying the image data itself This chapter provides instructions for creating text and graphical annotations arranging them and saving them with your images 6 1 The Select Tab The Select Tab iS 5 4 z lt Er Wie x gt Image Tools en Capture Adjust Select Measure View Image 10 7 ie 1 a i aema me e a a a H A el all Copy Von res Baits ions Burn a Ek Crop te gs oa x 4 3j amp Clipboard 1 Regions of Interest Annotate The Select tab contains those functions that allow you to annote an image examine a portion of an image or copy and paste to another workspace or application The functions in this chapter can be found on the Select tab s Annotate group Creating an Annotation Using the tools in the Annotate group y
27. Units area click the Name drop down arrow and select the units to which you want the pixel level measurements converted as shown below For example if the size of objects in the image is best expressed in terms of micrometers select Micrometer from the list Units ER Abbr um Mame In the Abbreviation text box enter the text you want to use as the abbreviation for the selected units For example a typical abbreviation for Micrometers is um as shown above The specified abbreviation will appear on measurement reports In the Pixel Unit group do one of the following ig Pixiunit Unitsipizxd X 177 0525 Si ciin a ze ES Y ie If you know the number of pixels per unit to specify 1 Inthe X spin box type the number of horizontal pixels it takes to represent one unit of length For example if you are converting to millimeters and 4 pixels equals one millimeter type 4 2 Ignore the Y spin box 3 17 The Capture Tab If there is a feature pictured in the image that has a known length value A 1 Click the From Image button in the Pixel Unit area A dialog box appears in the Spatial Calibration panel and a horizontal annotation line appears in the image 2 Click and drag the line over the reference object and then adjust the line s control points so that the beginning and ending of the line correspond to the beginning and ending of the
28. Viewing Tooltips Tooltips show the basic image information Path C Program Files Media Cybernetics MAG Capture Pro Images Paperseg Size 320 x 240 Type Monochrome 8bpp Frames 10 Calibrations To view an image tooltip move your mouse cursor over the image thumbnail Lock Images Q Capture Pro 7 enables you to lock two images together so that they zoom pan and scroll in tandem To use this feature 1 Open a pair of images in Q Capture Pro 7 2 Hold down the lt Shift gt key while clicking on the images in the Image Strip 3 Right click on on of the images in the Image Strip You will see a pop out menu Activate Close selected windows Save forReport Save for Analysis Lock selected windows Lock options Thumbnail size 4 Choose Lock Selected Windows The selected images are now locked together The pop out menu will change to Unlock 9 9 Customizing the Image Strip Activate Close Save forReport Save for Analysis Unlock eee oe M Thumbnail size 5 Choose the appropriate Lock Options Pan Scroll B foom Active Frame Auto Arrange Pan Scroll Zoom Active Frame Auto Arrange 9 10 Customizing the Image Strip Activating and Closing 1 Click on a thumbnail in the image strip to activate the corresponding window If the thumbnail represent a surface plot or 3D view or other viewer clicking will also update the thumbnail 2 To cl
29. about viewing and using histograms appears in Chapter 4 2 12 The Q Capture Pro 7 Application Window Status Bar Options control the information that appears in the status bar at the bottom of the Capture Pro workspace General options include image type and size frame number cursor position pixel values ROI information and calibrations are some of the choices You can also turn the calibrated cursor position on or off A sample status bar reflecting the options selected in the dialog above appears here Intensity Date and Time Calibration Frame Number Cursor Positio Pixel Value Image Viewer 2 13 The Q Capture Pro 7 Application Window Capture Options allow you to make changes to the image capture and preview settings General options let you reset the capture region to the default selection and apply the best fit display when the image is captured Preview options let you decide how to display the image preview before it is captured 2 14 The Q Capture Pro 7 Application Window Display Options allow you to set the unit of measure for angles and the number of digits that appear after the decimal point SS m Se eae Se 2 15 The Q Capture Pro 7 Application Window Quick Access Toolbar This toolbar located just above the tabs contains controls which are usable regardless of which tab is active You may add a copy of any almost any button or menu item to
30. and all profile features are removed from the images they were created on Using the Auto Trace feature Activating the Auto Trace tool for an ROI or Annotation will cause the Polyline tool in Line Profile to behave the same as the Auto Trace tool When you click on the Polyline feature and activate Auto Trace you will see the following dialog Tracing has not been started Tracing Speed 80 _ Edge Quality 20 d Noise Reduction Momentum 0 0 0 Smoothing 0 l ale slo sl Auto Start Tracing Auto Accept Final Trace Trim Leading Points Remove Redundant Points Monochrome Interpretation Assume Clockwise Use RangeMin RangeMax 4 Bound Trace by ROI Disable Tracing The message at the top of the dialog indicates if auto trace has started or not Tracing speed Use the slider to control the speed of the auto trace 4 9 Viewing a Line Profile 4 10 Edge quality Indicate the number of pixels to use for the edge trace Noise reduction Use the spin buttons to indicate the degree of noise reduction in the traced object Momentum Use the spin buttons to indicate the degree of momentum in the traced object Smoothing Use the spin buttons to indicate the degree smoothing in the traced object Check the box next to the auto trace feature that you want to use Auto Start Tracing If this box is checked the second mouse click made when creating a polygo
31. automatically resets the histogram display af Ft r 4 Best Fit Best Fit applies the best fit display to the image Auto Range automatically adjusts the black and white levels on the image 4 2 Viewing a Image Histogram Right click in the Histogram window to display the drop down menu ell Copy Graph Export Graph Histogram Dynamic tur Ew Levels Mey Optrons This menu lets you select a variety of options You can copy your graph or export it to Microsoft Word Excel or Powerpoint The View sub menu allows you to display the graph legend and statistics AXIS Axis Y Axis Y Log Legend Statistics You can adjust the threshold by using the right and left sliders As you move the sliders you will see the changes in the upper and lower limits reflected in the image as shown here Image Histogram l x 76 206 Red 10000 Green Blue sss BL Thro WL 0 0 64 128 192 32 96 160 224 Red Green Blue Meu a 2is2 ae AG1695 ot 22R ATE cee StDev 38 099 41 221 18 507 Min 80 79 145 Max 255 255 255 Sum 53627342 44653280 56101145 Sliders showing adjusted upper lower limits Image showing adjusted limits 4 3 Viewing a Line Profile Viewing a Line Profile 4 4 Line profile analysis allows you to collect the actual index values along a line that you define A profile plot shows the pixel positions of the line along the X axis and on the Y axis measures th
32. begin 9 Click Finish to conclude this part of the installation process 1 7 Installation Installing the Qimaging Capture Interface To acquire images with your imaging software you must first install the appropriate capture interface The capture interface installation will begin automatically after the Q Capture Pro 7 software installation is complete These instructions cover the installation of the QImaging Capture Interface 9 only You should also install the QCAM driver from Q maging The QCAM driver is available on the CD ROM provided with your camera and can be downloaded from the Ol maging website at http www qimaging com support downloads Recent improvements to the QCAM driver may affect compatibility with older software products If your system is already configured to use your Q maging camera in another software application please confirm compatibility with your software vendor prior to installing the latest version of the QCAM driver To install the QOlmaging capture interface follow these steps 1 Click Next to begin installing the Q maging l Ska motela capture interface oen e Cc Welcome to the Qimaging Capture Interface Setup The setup will install the Qlmaging Capture Interface on your computer Click Next to continue or Cancel to exit The capture interface is required for your imaging application to transfer imaging data from your Qlmaging camera through the QCam Driver QCam D
33. commands to the Quick Access Toolbar 1 Place your cursor along the buttom of one of the groups on the ribbon for example Annotate Coes s ESEA Eee wee Annotate 2 Click the right mouse button You will see a pop out menu Customize Quick 4ccess Toolbar Place Quick Access Toolbar below the Ribbon Minimize the Ribbon 3 Choose Add to Quick Access Toolbar The Annotate icon appears in the Quick Access Toolbar Deleting a Shortcut from the Quick Access Toolbar To remove a shortcut from the Quick Access Toolbar follow these steps 1 Place your cursor on the command icon that you wish to remove a gt Oe Mm air 2 Click the right mouse button You will see the following drop down menu Remove From Quick 4ccess Toolbar Customize Quick Access Toolbar Place Quick Access Toolbar below the Ribbon Minimize the Ribbon 3 Choose Remove from Quick Access Toolbar or press lt Control gt lt R gt 9 5 Customizing the Image Strip Customizing the Image Strip The Image Strip displays thumbnails of all opened images in the application and provides an easy way of activating and deleting images The Image Strip controls are located in the Workspace group on the View tab s ribbon Image Tools i DE Views Took Layout Workspace Q Capture Pro 7 opens the Image Strip automatically when you launch the application It appears on the left sid
34. desired destination Polygon Polyline This tool allows you draw poly vertices lines and polygons or freehand lines and shapes Note Whether this tool draws a polygon or a polyline depends on the current setting for the Connect Start End option To change the status of this option simply right click the mouse over the object after it is drawn and select AnnotationlObject Properties The Connect Start End option appears on the Polygon tab of the Graphic Object Properties dialog box To draw freeform lines and shapes Use this technique to create lines and shapes that Choh drag have smooth edges Hold the left mouse down while E ak release you draw with the cursor to create the desired and double freeform shape Double click to complete the shape chek To draw polyvertices lines and polygons Use this technique to create lines and shapes that are Pouble slekh Chek4 made up of straight line segments Click the left mouse button at each vertex including the Chek 3 beginning point of the polygon Double click to mP complete the polygon Bek 2 Creating an Annotation Auto Trace Use this tool to trace the outline of an irregular object in the image automatically Place your cursor on the object and click two points Q Capture Pro 7 automatically traces the object Point Position the crosshair cursor in the image where you want to place the point marker Click the mouse to place the point Delete Sel
35. flip left to right tool Original image Flipped image You may want to experiment with the other Rotate tools The tooltips contain additional information about these tools 5 16 Enhancing an Image Using the Canvas The drawing Canvas lets you add or remove space around an exisiting image an ba Resize Rotate Canvas Transform 1 To use the canvas click on the Canvas button You will see the drawing canvas as shown here 2 Use the Canvas size command from the drop down arrow to position the image on the canvas as shown here 3 From here you can add annotations and labels to the image on the canvas You can also crop the image by removing space around it 5 17 Enhancing an Image Converting Images Use the Convert group commands to transform an image to another color model to extract a specific color channel from a color image or to merge an active 8 bit gray scale image into a True Color image You might do this to perform editing or analysis upon the image in another model or to save the image data in a different model for use with an external program The Convert group is shown here Mono bpp i a Canvert To convert an image 1 Select the Destination image type from the drop down list WCE Mono 16bpp Mono FPoint Color 24bpp Color 36bpp Color 4 bpp Color FPoint 2 Click Apply lad Apply Scale Pixel Values Best Fit Pixel Values Source 21 242 de
36. images 3 1 using a digital camera 3 3 Channels group 5 25 5 29 Circle tool 6 4 Circle Tool 4 7 Class group 8 5 8 6 Closing Images 9 11 Color changing the color of an annotation 6 10 changing the color of an ROI 7 9 Color channels 5 25 Color Composite dialog box 5 20 Color group 5 18 5 19 Commands making shortcuts for 9 2 Context menu 9 7 Contrast adjusting 5 27 Contrast adjusting with mouse 5 27 Conventions used in this guide viii Converting Images 5 18 Copy Tools 4 6 Cursor panel 8 9 Custom Controls 3 8 Customize Quick Access Toolbar 9 3 Customizing image strip 9 6 user Interface 9 1 Cut copy paste 6 5 D Dark Background 5 30 Data Table 8 6 8 10 Date and Time 3 9 Defining a new spatial calibration 3 14 an ROI 7 3 Delete 6 5 Delete ROI 7 5 Delete Tool 4 7 Deleting an ROI 7 8 Deleting an annotation 6 10 Details Panel 2 3 Dialog boxes Color Composite 5 20 Digital camera 3 2 setting up 3 2 Display group 5 25 5 27 5 28 Display Options 2 15 Display Range feature using 5 27 Drawing Properties 6 11 7 10 Drop Down Menus 2 20 Dye Tint 5 19 Dynamic auto range 3 4 E Ellipse tool 4 7 6 4 for defining ROI 7 3 Enhancing an Image 5 13 Export Measurements 8 11 Extract background image 5 31 Extract Command 5 19 F Features of Q Capture Pro 7 vii File Format Specifications 10 1 File Formats 10 1 AVI 10 1 Bitmap 10 1 FITS 10 1 JPEG 10 1 T
37. laws provisions thereof but shall not be governed by the Uniform Computer Information Transactions Act as adopted in Maryland or the United Nations Convention on Contracts for the International Sale OF Goods vii oer Should any part of this agreement be declared void or unenforceable by a court of competent jurisdiction the remaining terms shall remain in full effect 4 Failure of Media to enforce any of its rights in this agreement shall not be considered a waiver of its rights including its rights to respond to subsequent breaches G This Agreement is the complete and exclusive statement of the agreement between you and Media and supersedes any proposal or prior agreement oral or written any other communication between you and Media relating to the subject matter of this Agreement Should you have any questions concerning this Agreement you may contact Media in writing at the address above BY USING THIS SOFTWARE YOU ACKNOWLEDGE THAT YOU HAVE READ THIS AGREEMENT UNDERSTAND IT AND AGREE TO BE BOUND BY ITS TERMS AND CONDITIONS viii Table of Contents Table of Contents Preface Before YOU Begin 1scccsssccnncccsensnsnnecennssensesensesensnsenessenanss vii Product F ealUreS nesa Weeretiaubestueaube eee Meee niseubarnneaies vii Conventions Used in this Guide ccccccseseecececeeseeeeseeeeeeeessaaeeeeeeeeeess viii Chapter 1 Installing Q Capture Pro 7 w ssssssssssssssesesssesesesesesesen 1 1 System
38. menu Slider bar Selection tool Undo Redo buttons Image Properties button Delete buttons Image Histogram button Red Green Blue and White Tint buttons m The Slider Bar allows you to adjust the size of the image by moving the slider to the right or left E The Undo and Redo buttons let you reverse or repeat the last three action The Histogram button opens the image histogram m The Image Properties button displays information about the image in a table below the image m The Red Green Blue and White tint buttons apply the appropriate color to the image m The Delete and Delete All buttons remove tints from the active image or all open images 5 8 Zooming Images Zooming Images The zoom and pan controls allow you to resize the image or a portion of the image There are several ways to zoom in and out on an image that is to display it at different levels of magnification You can Use the Zoom button in the Zoom group Use Local Zoom Use the wheel on your mouse if available Use the scale bar Zooming by Using the Zoom Button This method of zooming allows you to zoom in on specific areas of the image 1 Click the Image tab from the Q Capture Pro 7 menu bar The Zoom group is displayed a py EA Foom Par Local Lock Zoom zoom Click on the Zoom button lt Do one of the following Position your mouse over the area of the image you want to magnify and cl
39. reference object TIP For greatest accuracy it is often helpful to zoom in on the reference object To zoom in on the reference object click on the Zoom Zoom tool from the View tab s ribbon With the Zoom tool selected position the cursor over the reference object and click Repeat these steps until the object is large enough to determine its starting and ending pixels 3 In the Reference length units spin box type the known length of the pictured object 4 Click OK The Spatial Calibration dialog box is redisplayed and the calculated pixels unit value appears in the X and Y spin boxes If you have an image of a micrometer you can calibrate the image automatically 1 Click the Auto Calibrate button 13 in the Pixel Unit area 2 A dialog box appears over the image of the micrometer 3 Click OK and the calibration will be calculated automatically 8 Select the Advanced Options dialog box on the Spatial Calibration panel 3 18 The Capture Tab Conversion factor in meters Aspect ratio Origin X pixels Ongin pzels Angle offset degrees 9 Inthe Apect Ratio area do one of the following If you know the aspect ratio value to specify or if there 1s no aspect ratio problem to correct for 1 Inthe Aspect Ratio spin box type the aspect ratio value to be used For example if there is no aspect ratio problem to correct for enter as Re Q Capture Pro 7 automatically ad
40. selected using Red Green Blue checkboxes or by clicking the Channels button Using the Saturation Warning This is a sample of a partial image with the high saturation areas visible The Reset button sets the controls back to their default values 4 12 Chapter 5 Working with Images This chapter introduces you to image processing in Q Capture Pro 7 It provides instructions for opening saving and enhancing images through Q Capture Pro 7 When you start Q Capture Pro 7 and the Welcome screen is turned on you will see the list of your recent images in the Recent Documents pane Q Teu h Ciapus Pes Wiehe Q CAPTURE W Sroa da ii war Opening an Image You can click the name of the image and it will automatically open in the Q Capture Pro 7 workspace Lanira Ane at CJ 0c Un fale fi If the Welcome screen is turned off follow the directions below for opening an image 5 2 Opening an Image Opening an Image To open a saved image 1 Click the Application button a T Trn x Ly x lt P EA T az F Capture Adjust Select Measure View Application button The pull down application menu appears 2 Choose Open L P Da Fy T c T n Image Too eS Recent Documents New 1 NUCSTAIN TIF P a save d EB veas rll ls Reload 2 Print sgoe Open b 9 Options kj Exit Q Capture Pro 7 3 Select the type of image file t
41. slider and a fixed zoom control list allow you to control the size of the image in the workspace Right click the fixed zoom control to see the control list 5 10 25 50 79 125 150 175 200 250 Scale to Best Fit Scale to Width Scale to Height Scale to Best Fit automatically adjusts the image display to show the best fit Scale to Width automatically adjusts the image to the width of the workspace Scale to Height automatically adjusts the image to the height of the workspace Q Capture Pro 7 allows you to enable or disable the Status Bar fields Right clicking on the Status Bar displays a menu that allows you to select what type of data you want to display in the status bar Image type Image size Frame number Frame time Cursor position Pixel value AOI extents AQT size Intensity calibration unit Spatial calibration unit Click the cursor on the field you wish to enable or disable Enabled fields are checked The Q Capture Pro 7 Application Window Tabs and Ribbons Tabs are used to display different groups of functions The tabs are arranged in a progressive left to right order following a common workflow where a person using the application acquires an image from a source adjusts it in some fashion selects objects in the image for study measures properties of those objects visualizes those objects in multiple potential contexts then shares this information with others The defaul
42. to adjust these settings Gain These sliders control the digital gain for both preview and acquisition Preview and acquire may be adjusted individually or they may be locked together to set both simultaneously Click the Locked button to make the gain settings equal for both preview and acquisition Offset This feature allows you to adjust the vertical and horizontal offset of the capture device for both preview and acquisition Preview and acquire may be adjusted individually or they may be locked together to set both simultaneously Click the Locked button to make the offset equal for both preview and acquisition Use the Gain controls to increase or decrease the brightness of the image This control makes all pixels uniformly brighter or darker Gamma These sliders control the gamma for both preview and acquisition Preview and acquire may be adjusted individually or they may be locked together to set both simultaneously Click the Locked button to make the gamma settings equal for both preview and acquisition The Capture Tab Next expand the Time Lapse Controls O Enable Movie Acquire Number of Frames 2 Hours Mins SECS MSecs Time Interval oS oS 16 Ja E E Burst Frames 0 Enable Burst Total Duration 00 00 01 000 hi The Time Lapse Options contain controls for capturing sequences either as fast as possible or with a time delay between frames The number of frames is limited to a minimum of two frames You can s
43. to complete the shape To define a polygon shaped ROI Use this technique to create polygons that are made up of straight line segments Click the left mouse button at each vertex including the beginning point of the polygon Double click to complete the polygon Chea daag around and back release and double chek Double chiekh Chek d Chek 3 Chea I Chea 2 Auto Trace Use this tool to trace the outline of an irregular object in the image automatically Place your cursor on the object and click two points Q Capture Pro 7 automatically traces the object Display Options Use this button to see the Display Options panel Optiors Delete Selected Delete All Use the buttons to remove one or more of the ROIs from your image Multiple ROIs Click this button to place mutiple ROIs on your image This option is always on by default Display Options Use this button to see some additional ROI options Pees 5 i Invert ROI Click this button to invert the ROI for example a black ROI on a white background XOR Pixels Click this button to include or exclude pixels in overlapping ROIs This option is always on by default Order Use these buttons to arrange your ROIs from back to front or front to back Placing Multiple ROIs on an Image Placing Multiple ROIs on an Image If you want to define more than one ROI in a single image you can use the Allow Multiple ROIs tool Click on the Allow Mult
44. v ERIA Sh Saturatic Analysis Publication Movie Cameras Setup Optical Characteristics Calibration Intensity Quick Save redictive Calibrations Camera parameters Camere Manufacturer Photometrics Camera Model Cascade 128 z Pixel Size RUGU microns Sensor Size Wx H 2X 25 pixels Calibration Suffix Cascade 128 Optical parameters L W Lenses Zooms E 10 generic F 20 generic E 50 generic 100x generic Optical Coupler 1 00 Calibration parameters Show calibration dialog Cancel You will see the Create Predictive Calibration dialog Each camera is defined by the Manufacturer and Camera Model fields Select your camera manufacturer and model from the drop down lists The pixel and sensor size is determined by the camera make and model If you want to set the pixel and sensor size manually for the new calibration choose Other in the list of cameras The Calibration prefix is also defined automatically the camera selection You may type in a new prefix in the space provided Select the lenses from the calibration from the Lenses panel 3 21 The Capture Tab All active lenses are selected by default so if some lenses should not be calibrated uncheck them using the v button to select deselect a lens Add additional Zoom settings in the Zoom panel This list is empty by default Enter the calibration units in Units field under the Calibration param
45. vendor prior to installing the latest version of the PVCAM driver To install the PVCAM capture interface follow these steps 1 Click Next to begin installing the Q maging PVCAM Capture Inter PVCAM capture interface gt a mur Se HF Welcome to the Qimaging PVCAM Capture Interface Setup The setup will install the Qimaging PVCAM Capture Interface on your computer Click Next to continue or Cancel to exit The capture interface is required for your imaging application to transfer imaging data from your Qimaging PVCAM camera the PVCAM PVCAM version 2 9 11 or later is required to ensure correct oper atior l 1 19 Installation 2 1 16 You will see the QImaging PVCAM Capture Interface End User License Agreement End User License Agreement Please read the following license agreement carefully IMPORTANT PLEASE READ CAREFULLY LICENSE AGREEMENT THIS LICENSE AGREEMENT AGREEMENT IS BETWEEN YOU THE END USER AND MEDIA CYBERNETICS INC MEDIA IT GOVERNS THE USE OF THE SOFTWARE PROGRAM MEDIA AND DOCUMENTATION KNOWN AS Qimaging PVCAM Capture Interface THE PRODUCT IF YOU USE THE PRODUCT THEN YOU AGREE TO THE TERMS OF THIS AGREEMENT IF YOU ARE NOT WILLING TO BE BOUND BY THE TERMS OF THIS AGREEMENT PROMPTLY RETURN THIS PACKAGE TO THE PLACE OF PURCHASE WITH A COPY OF THE RECEIPT AND YOUR H CFNSF FFF WII RE RFFLINDED V 1 accept the tems of this License Agreement Check the box to acce
46. yi ae ar 29 auto pone ADO FPS a tye Lie epiur Sp Lang r Hane iimis keiss Active Content Panel Sepee fan T SS TAP egaa bk gt jibe a RE Wo ISG AM jy pe ae E or live aw Cape The Q Capture Pro 7 Application Window _ Details Panel dtait E Milasa i pj M is date born saje Eapodeed ee Birra l B aiara yal E micer Lb Gray ri l Gain oe A i SE Otek BE n A 0 Y amma Bei See Fre leeiond Time Lapte Ope The following sections explain these features of the Q Capture Pro 7 application window in greater detail The Q Capture Pro 7 Application Window The Q Capture Pro 7 Ribbon The Q Capture Pro 7 ribbon contains commands that affect or change the content in the main workspace The overwhelming majority of the commands in the ribbon are buttons In many cases gallery controls roll up the effect of many button clicks and dialog settings into a single click The ribbon displays commands organized into tabs and groups The Capture tab s ribbon is shown below ir m w a TO wee Capture Adjust Select Measure View af me AJ Auto Exposure 0 00 FPS mr i wo Fse L none ai Live Capture Setup a 6 s X ti pt None a i Cameras Setup Optical Characteristic Calibration Intensity i el be ation Analysis Publication Movie x Quick Save The different ribbons will be ex
47. you to change the font to be used for rendering text Size 20 Allows you to change the font size to be used for rendering text E la H gt Allows you to make the text bold italics underlined or strike though a letter When you have made the desired changes close the Annotation Display Options dialog box The changes are applied to the selected annotation Setting the Default Appearance for Annotations The default appearance attributes for annotations are set by designating the appearance attributes of an existing annotation as the default for future annotations of that type Note that defaults exist independently for each type of annotation you can draw rectangle rounded rectangle ellipse polygon point and text To set the default appearance for an annotation type 6 12 Click the Selection tool W from the Annotate group In the active image click on an annotation of the type for which you want to set appearance defaults For example if you want to set the default appearance for ellipse type annotations click on an ellipse type annotation in the active image If one doesn t exist draw one Setting the Appearance of Annotations Click the Display Options button Ei in the Annotate group You will see the Display Options dialog box along the right side of the workspace Display Options l x Ellipse Rectangle Z Polygon Label Border po Visible Width 1E Ang
48. 5 13 Rolaing an VAG Sadie ace Sve vie ncn A N van ane ual 5 15 LISI IOC anv ASen a E T 5 17 CONV ENING Mages smaa S a a NE 5 18 Using Color Group COMMANAS ccccccccsseeeeeeeeeeeeeaeeseeeeeeeeeeaaeeeees 5 19 DYS TM NMAGCS a roir e E T 5 19 Using Color Compose zanesie a eee me niaeeas 5 20 Pseudo Coloring AN IMAGE nnnoannnnnnnneennnnnnnnesennrnnneesennnrrrrensennnnnni 5 22 Adjusting Color Channels eeniseannei ae 5 25 Changing the Display Range ccccccccsssseeeeeceeeeeeeesueeeseeseaeeeeeees 5 27 Adjusting Brightness Contrast and Gamma ccccceeeeseeeeeeeees 5 27 Resetting the IMage cccccccccssseceeceeseecseseeessesseesseueeesseegeeeseeeeessaes 5 29 Table of Contents Making Enhancements Permanent ccccsscceccsseeeeeceeeeeseeseeesaeeees 5 29 Correcting the BACkQround ccccccceeecccceceeeeeeseeeeeeeeseeaeeeeeeeeeeessesaeeees 5 30 PDO IV IG IFINMGUS anae a a a i 5 32 image SSQUCII COS usis E A 5 34 Chapter 6 Using Annotations 111ccccsesecccesnencneenneneneensnneneenenneneees 6 1 THE SEIS CET AD Gira a steeisateemiushcartetesededuadubades 6 2 Creating an Annotation eesin Give val EE A AEE ead 6 2 Airanging ANIM OLATOINS cener a aa a 6 7 D leting air Anmnotalon iene e a 6 10 Setting the Appearance of Annotations ccccccceeceseseeeeeeeeeeeaaeeeees 6 10 Changing the Appearance of an Annotation ccccccccccsseceeeeeeeeeeees 6 10 DAVIMG
49. B You will see the measurement labels change to the font you select Use the color style and size buttons to make additional changes to the measurement labels In the Labels panel select the text position and units of measure for the measurement labels AA S Text Name And Meas Position Center x i Units Abbreviation Decimal Points 2 s The choices for Text include None no labels Name measurement object name is displayed Measurement the label measurement is displayed Name And Measurement full name and measurement are displayed Units of measure and measurement abbreviations can be added to the measurement label by checking the appropriate boxes You can also indicate the number of decimal points for each measurement Here is an example of measurements with labels Manual Tagging Qe mm I an Eg P 2 35 mm BOad nun 1mm 90 mu Use the Outlines panel to define the style of the each object class and shape aean Outlines _ _ ___ Style _ End Points Width 1 L Filled The Cursor panel lets you customize the measurements cursor Cursor _ Cross Bars LJ Crosshair A Tooltip Cross Bars are useful adjusting line ends on the features They are only active in the line editing mode Crosshair helps you to center horizontal or vertical measurements Tooltip shows measurement data on the image when you are moving the
50. CY of Style es is ii Visible Cli Option Z Zoom withImage Opacity Oha Layer Layer 0 Nid i Start Frame 1 1 End Frame Saveas Default Use the border fill and other options to change the appearance of the annotation Drawing Properties Some common controls available for changing the appearance of graphical annotations are described below Border Allows you to change the visibility color width and line style of a border around your annotation Fill Allows you to choose the fill color for your annotation including opacity and or transparency Options These options allow you to change the size of the annotation when the size of the images changes adjust the opacity transparency of the annotation and layer annotations on the image If you are working with an image sequence you can specify the first and last frrames where the annotation will appear Color Click on the Color button to change the color of the bounding box border or fill or to change the color of the text Zoom with Image Check this box to make the annotate larger or smaller as the image is resized 6 11 Setting the Appearance of Annotations Label Properties Some common controls available for changing the appearance of label annotations are described below Te Mew Label Arial 15 a pima E Text Click on the Text tab to change the text of the annotation Font faria 7 Allows
51. Click the Settings menu item to display the Advanced settings in the panel at the right side of the screen Simulation Settings i x or Live me Capture CaptureContos Capture Enhance Tmelapse Options Capture Region Color Custam Controls Naming Options Saveto File Options Date Time Stamp Options Settings Files Click the arrow to expand the Capture Controls as shown here RT ed Hee as A le eae aa a 199 980 Milliseconds Ee _ _ E Auto Exposure Ol Adjust Exposure for Binning 23 Binning ins S g towe From here you can adjust the exposure time for capture and set the binning and bit depth The other settings can be expanded and adjusted in the same way 3 5 The Capture Tab Important Note Preview and acquire settings may be adjusted individually or they may be locked together to set both simultaneously This option appears in many of the Capture controls Click the Locked drop down arrow to see the following controls Acquire Caniral T Preview Control Locked When Locked is selected the preview and acquire capture settings will be identical and locked together To adjust the settings individually select either Acquire Control or Preview Control From the Settings panel expand the Capture Enhance Controls Gain y ___ _ 1 D Ofset C O uH Yamma ae uH g Locked m Use the sliders
52. Edit button at the far right You will see an editing panel displayed on the right hand side of the workspace 3 From this panel you can edit or change the lens used to capture your images 3 12 The Capture Tab Using Spatial Calibrations Using a spatial calibration Q Capture Pro 7 s pixel level measurements can be converted to any unit of measurement Spatial calibrations can also correct for irregularities in the image s vertical and horizontal spacing There are two primary components to a spatial calibration m Pixels per Unit Aspect Ratio Pixels per Unit A spatial calibration tells Q Capture Pro 7 the size to which a given image is scaled just as the key of a roadmap tells us how many millimeters of image length represent one mile Likewise the spatial calibration tells Q Capture Pro 7 how many pixels of image length represent some more meaningful unit of length in the image for example one micron millimeter or inch To create a new spatial calibration you must either know the number of pixels per unit to specify for the image or there must be a feature pictured in the image that has a known length value in terms of the units of interest If a feature of known length is pictured Q Capture Pro 7 can calculate the number of pixels per unit by determining the number of pixels it takes to represent the known length Companies often plant an object of known length into images for this purpose Aspect Ratio
53. IFF 10 2 File Options 2 10 Filters 5 32 5 33 FITS file formats 10 1 Font changing in an annotation 6 10 changing in an ROI 7 9 Index G Gain 3 6 Gamma 3 6 Gamma adjusting with mouse 5 27 Graphic Object Properties 6 4 6 6 6 12 7 9 7 11 graphical annotations 6 3 Group tab Color Composite 5 20 Group Ungroup 6 5 Grouping annotations 6 8 Groups 2 20 H Hide Measurements 8 11 Hide Statistics 8 11 Histogram window 4 3 I Image Histogram 2 12 3 17 4 1 4 2 viewing 4 2 Image Histogram Options 2 12 Image Navigator 5 12 Image Options 2 8 Image Sequences 5 34 Image Strip 2 21 9 6 Image tab 2 20 5 9 5 11 Images adjusting brightness contrast and gamma 5 27 capturing with a digital camera 3 3 opening 5 3 panning 5 11 resetting 5 29 zooming 5 9 installation requirements 1 1 Installing PVCAM Capture Interface 1 15 installing Q Capture Pro 7 1 3 Installing Q Imaging Capture Interface 1 8 Intensity 4 1 Index 3 Index Intensity Features 3 27 Invert ROI 7 5 J JPG file formats 10 1 L Label Properties 6 12 7 11 Labels Measurement 8 8 License agreement 1 5 1 9 1 16 line measurements 8 4 Line profile 3 27 Line Profile 4 1 4 4 4 9 Line Profile Options group 4 9 Line Profile ribbon 4 6 Line Profile Tools 4 6 Line Tool 4 6 Live Preview 3 4 Load Measurements 8 11 Local Zoom 5 10 Lock icon 3 6 3 7 Lock Images 9 9 Log group 4 9 Look Up Ta
54. IN TIF 2 ORCUI TIF Click the name of the file to open it You can click the push pin icon next to a recently opened file to pin the file name in the list Click the push pin icon again to unpin the name in the list Zo The Q Capture Pro 7 Application Window Q Capture Pro 7 Application Options The Options button on the Application Tools dialog allows you to customize the user interface to reflect your personal preferences Clicking the Options button displays the following dialogs To turn a feature on or off check the box next to the name of the feature or select an item from the drop down menu Qa ke CBO m z Image Too Recent Documents 1 NUCSTAIN TIF Options ka Exit Q Capture Pro 7 Options button 2 6 The Q Capture Pro 7 Application Window The Options button on the Application dialog displays the Options dialogs These options allow you to set preferences for many aspects of the program and its features To turn a feature on or off just click its name and a drop down menu or check box will appear Popular Options provide an opportunity to control how certain workspace options behave The Image Options on this page allow you to set the preferences for closing modified images and reset the workspace to its default configuration TT The Q Capture Pro 7 Application Window The Image Options page displays information about the image in the workspace General options permit you t
55. OI 1 Click the Selection tool from the ROI group 2 Click on the ROI that you want to change The ROI is highlighted oe 3 Click the Display Options button ae in the ROI group You will see the Display Options dialog box in the panel along the right side of the workspace Display Options H Ellipse Rectangle Pabgon Label SEES M Z Visible E Width Angle cal siyle j Fm Visible B SSS eS A Zoom withImage Opacity 3 1 Layer Layer 0 e Start Frame 1 2 End Frame 1 Save as Default 4 Use the controls available through this dialog box to change the appearance of the ROI Setting the Appearance of ROIs 7 10 Drawing Properties Some common controls available for changing the appearance of graphical annotations are described below Border Allows you to change the visibility color width and line style of a border around your annotation Fill Allows you to choose the fill color for your annotation including opacity and or transparency Options These options allow you to change the size of the annotation when the size of the images changes adjust the opacity transparency of the annotation and layer annotations on the image If you are working with an image sequence you can specify the first and last frrames where the annotation will appear Color Click on the Color button to change the color of the bounding box border or fill or to c
56. Q CAPTURE P27 USER MANUAL Dea E a DIGITAL IMAGING MADE EASY COPYRIGHT NOTICE Copyright 2010 2013 Media Cybernetics Inc All Rights Reserved TRADEMARK ACKNOWLEDGMENTS Q Capture Pro 7 is a registered trademark ofQImaging Inc All trademarks in this document are trademarks or registered trademarks of their respective companies IMPORTANT PLEASE READ CAREFULLY LICENSE AGREEMENT THIS LICENSE AGREEMENT AGREEMENT IS BETWEEN YOU THE END USER AND MEDIA CYBERNETICS INC MEDIA iT GOVERNS THE USE OF THE SOFTWARE PROGRAM MEDIA AND DOCUMENTATION KNOWN AS O Capture Pro 7 THE PRODUCT Ez if YOU USE THE PRODUCT THEN YOU AGREE TO THE TERMS OF THIS AGREEMENT IF YOU ARE NOT WILLING TO BE BOUND BY THE TERMS OF THIS AGREEMENT PROMPTLY RETURN THIS PACKAGE TO THE PLACE OF PURCHASE WITH A COPY OF THE RECE LPI AND YOUR LICENSE PEE WILL BE REFUNDED Media licenses use of the PRODUCT which may be manufactured and distributed by Media or by a third party either the Manufacturer You the end user assume responsibility for the selection of the PRODUCT to achieve your intended results and for its installation and subsequent use GRANT OF LICENSE Media hereby grants you a non exclusive license to use the PRODUCT in object code form only upon the terms and conditions contained in this Agreement You may 1 Use the PRODUCT on the number of workstations for which you have purchased PRODUCT l
57. REQUIFEMENHS Seen eiii 1 2 Installing Q Capture PrO Z i sccticcueleie at olsitint a alobee ad tees 1 3 Installing the Qlmaging Capture Interface ceeeeeeeeeeeeeeeeeeettees 1 8 Updating Your Q Capture Pro 7 Product ccccccceeeeeeeeeeeeeeeeaaeees 1 15 Software Registration and Updates ccccccceeeceeeeeeseeeaeeesseeeeeees 1 15 Finding and Receiving Updates cccccssseeceeeeeseeceeeseeeeeeeesaaeeeeees 1 18 Chapter 2 Getting Started iiccniiioi rts 2 1 The Q Capture Pro 7 Application Window scccceeeeeeeeeseeeeeeeeeees 2 2 The Q Capture Pro 7 Ribbon cccccccesseeeeeeeeeeeceaeeeeeeeesesseaaeeeeeeeeeeeeas 2 4 The Q Capture Pro 7 Application button cccccseeeeeeeeeeeeeeeeeeeeeeeeeeeees 2 4 Q Capture Pro 7 Application Options ccccccccccccccessseceeeeeeeeeeeeeeeeeees 2 6 Laps and RIDDONS retreated cata EEA OER 2 18 VOUS Arene A a 2 20 imMage ets 0 earn te eae ete cei em Pg ce en eee 2 21 vii Table of Contents vill Chapter 3 Capturing IMages ssccssssccssssssnenensensensnssnseensesensens 3 1 TNE Capture Na iseaioscin tetantvictacs puedo eiaeost dl Actos ieee beghetecleealiteuee 3 2 WSingia Digital Carne tal se Se Sra See ate Sete See raat lee ese te Pee 3 2 Setting up a Digital Camera cc cccececccecceeeeeeeeeeeeeeeeesaeeeeeeeeeesseesaaeees 3 2 Basic Steps for Capturing Images Using a Digital Camera 3 3 Using A
58. S Flex Image Transport System format JPEG UPG JPEG JP2 JPF JPEG files will have a JPG or JPEG file extension Q Capture Pro 7 supports all standard varieties of the JPEG file format including JPEG 2000 10 1 Appendix A File Format Specifications Portable Internet Graphics png Portable Internet Graphics are a common format for all types of graphics The extension is PNG Q Capture Pro 7 supports all types of PNG files Sequence SEQ Q Capture Pro 7 supports multiple image TIFF files with the SEQ file extension TIFF TIF TIFF Q Capture Pro 7 supports all known varieties of the TIFF the Tagged Image File Format file format including most multiple image varieties and PowerPoint TIFF files 10 2 Index X AVI 10 1 BMP 10 1 DIB 10 1 FIT 10 1 FITS 10 1 FTS 10 1 JP2 10 1 JPF 10 1 JPG 10 1 RLE 10 1 Seq 10 2 TIF 10 2 A Acquiring images 3 3 Activating with Internet access 1 21 Activating Images 9 11 Adding shortcuts to the Quick Access Toolbar 9 2 Adjust tab 2 18 5 13 5 27 5 28 5 32 Adjusting brightness contrast or gamma using the mouse 5 28 Advanced Filters 5 33 Advanced LUT Adjustment 5 25 Advanced Settings 3 5 Annotate group 6 2 6 7 6 8 6 9 6 10 6 12 6 13 Annotation Display Options 6 5 7 4 Annotations 6 1 creating 6 2 deleting 6 10 grouping 6 8 moving 6 7 ordering 6 9 resizing 6 8
59. UANNO LAM ONS ei a e EEES 6 14 Chapter 7 USsing ROIS moarnen aa aaa a a EERE 7 1 VV at Sa RON aar E A 7 2 Deming an RO Monee titled a r eild teeth Adderall os A ot casts 7 3 Placing Multiple ROIs on an IMage ccceccceeeseeeeeeeeeseeeeeeeseeeeeeeeeeaaees 7 6 Aranon ROIS eairariontai e a eS e n e O 7 6 Deleting anr RO erre a dust ade daubeamscmaaecuutenatduvednaedns 7 8 Setting the Appearance of ROIS cccccecccccssssseeeessaeeeeeeeseeeeeeeessaeeeees 7 9 Chapter 8 Measuring Objects in Images 111sccccssssensssesseseneees 8 1 Overview of the Measurement Feature ccccceceeceeeeeeeeeeeeeeeeeeeeeeeeees 8 2 Making Manual Measurements cccseecceceeeeeeeceeeeecaeeeeeeseeeeesaaeeeesanaes 8 3 Measuring Features in an IMage cccsccccccsesceeeeeeeeeceeeeeeeeeeeesseeeeesaaaes 8 3 LARNACA GINA Gi cena aioe ues acto geht E O Satautiomac dae 8 6 Setting the Default Appearance for Measurement Labels 0 8 7 Viewing Measurement Data ccccecceccessececceseecceeseecseseesseuseeeenaaees 8 10 Viewing a Data Tableauen a edie ieee 8 10 Chapter 9 Customizing the User Interface ccccsssscsennsseseene 9 1 Using the Quick Access Toolbar ccccccccccssssssessseeeeeeeeceeeeeeeesseeeaeeeeeees 9 2 Customizing the Image Strip cee ececccccecessseeseceeeeeseeeeeeeceeseessaeaeseeeees 9 6 Table of Contents Preface Before You Begin Welcome to the Q Capture Pro 7
60. al image fil Quick Print kd aoe _ Bemove 4 Print the active document to tl la Save default printer IF Undo D Image Properties z Open Images A Close All Views se Open one or several imaat 4 Ml gt 4 il ia di _ Place Quick Access Toolbar below the Ribbon OK Cancel 3 Select a group of commands to display in the list box In the list box box highlight the command you want to add to the Quick Access Toolbar 5 For example if you regularly use the Paste command in Q Capture Pro 7 you can make a shortcut that performs a paste by adding a Paste command and icon to the Quick Access Toolbar If you are unsure which commands you need choose All Commands Customize Quick Access Toolbar Choose commands from T Switch to MDI 5d Close lag New IF Undo Open E Redo Print Open Images Redo Add gt O Add gt gt Open one or several image fil Quick Print Reload kd Remove _Bemove cod Print the active document to tl lee Save default printer IF Undo P Image Properties z Open Images A Close All Views a Open one or several imaq 4 il dh 4 il Lia di _ Place Quick Access Toolbar below the Ribbon 6 Click the Add button and the command appears in the right hand list box 7 Click on the OK button The shortcut is added to the Quick Access Toolbar 9 4 Using the Quick Access Toolbar You can also add a group of
61. amined in more detail later in this chapter The Q Capture Pro 7 Application button The Application button is located in the upper left corner of the application window The Application button menu contains commands that operate on the document as a whole such as Print and Save as well as other basic functions Sx ye CB 0x Application button Recent Documents New 1 NUCSTAIN TIF Recent Documents Open window Save Save As P List of F Commands Reload Options kg Exit Q Capture Pro 7 2 4 The Q Capture Pro 7 Application Window Clicking this button displays functions that are always available Open Image Opens an exisiting image You may also open a file as a sequence and as a best fit image Save Saves the active image s to a file Save As Saves the active image as new file type Reload Reloads the current active image from disk overwriting any changes which were made to the current file since it was last saved Print Selects a printer number of copies and other printing options before printing o Quick Print Send the image and any overlays to the default printer without making any changes o Print Preview Preview and make changes to pages before printing Close Closes the active image Options Lists the different options available to you Exit Closes the application The Recent Documents window lists the files that you opened recently Recent Documents 1 NUCSTA
62. an indicate the starting number and the number of digits Min digits lets you specify how many digits the sequence number will use 1 e 001 002 etc The Sample at the bottom of the dialog shows you how your image name s will appear To save your captured image to a file select Save to File Options Save captured images to file CADocuments and Settings All Use Browse Format TFF Image tif tif Compression None m Quality Prompt for filename after capture Kad The Save to File Options controls contains options for automatically saving a captured image to a file on the hard drive The folder path file format compression if applicable quality if applicable can be specified Also there is a checkbox for prompting the user for a filename every time an image is captured Some of the file types supported for Save to File are TIFF Image TIP Portable Network Graphics PNG JPEG Image JPEG 2000 JPG Image Pro Sequence SEQ Movies AV and Windows Once you have checked the Save captured image to file box use the Browse button to select the file and folder location Use the Date and Time Stamp Options to place a date and time on captured images 3 9 The Capture Tab 11 3 10 ol Available Formats Thursday March 18 2010 March 18 2010 3 18 10 2010 03 18 Text Gee pix tf Initial Text position posi
63. bbon select the Spatial Calibration group D S v Aas Besa eal Click the Calibration options icon to open the Spatial Calibration dialog box in the panel on the right Spatial Calib x g Spatial Cal 3 Name Millimeter Abbr Pixel Unit Pixiunit Unitsipixe X 177 0525 a B Ato calibration y a ooo Adancedoptions By default the Spatial Calibration panel contains contains the Spatial Calibration basic view and Advanced Options dialog boxes The Advanced Options dialog box is collapsed by default Click the header in either of the dialog boxes to expand or collapse the dialog box Click the Calibration Name drop down arrow and select the set of calibration values you want to apply to the image If you want to use the default values The Capture Tab select none Any calibration sets that were loaded or created since the application window was last opened will be listed in this list box You will not be able to enter calibration values until a name other than none has been selected in the Name list box If none is the only set listed you can create a set by clicking the New aF button and specifying its calibration values By default the name Spatial Cal 0 will be assigned to a new calibration set however you may change this to a more descriptive name if you d like Type over the default name Spatial Cal 0 with a name that is more meaningful In the
64. ble 5 25 Lookup Table for image enhancements 5 27 M Macros making shortcuts for 9 2 Manual Measurements using 8 3 Manual Tag tool 8 6 Manual Tagging 8 6 Marker Options 3 25 marker calibration 3 24 Measure tab 2 19 Measurement Data Viewing 8 10 measurement labels 8 7 Measurements Index 4 introduction to this feature 8 2 Measurements group 8 4 8 6 8 7 8 10 Measuring Features in an Image 8 3 Meaurement Options 8 7 Merge command 5 19 Mouse wheel using to zoom 5 10 mouse to adjust brightness contrast or gamma using 5 28 Movie Acquire 3 7 Moving an annotation 6 7 an ROI 7 6 Multiple Document Interface 2 9 Multiple ROI tool 7 5 Multiple ROIs tool 7 6 N Naming Options 3 8 O Offset 3 6 Opening a Recent Document 5 5 Opening an Image 5 3 Options 1 23 2 6 2 7 Application 1 23 2 9 Capture 2 14 Display 2 15 File 2 10 Image 2 8 Image Histogram 2 12 Paste 2 11 Popular 2 7 Status Bar 2 13 Order ROls 7 5 Ordering annotations 6 9 Outlines panel 8 9 Overview panel 2 3 P Pan Tool 5 11 Panels Layout 2 9 Panning images 5 11 Paste Options 2 11 Pixels per Unit 3 13 3 14 Point tool 6 5 Polygon tool 6 4 for defining ROIs 7 4 Polygon Tool 4 7 Popular Options 2 7 Predictive calibration 3 21 Profile tools 4 8 Pseudo Color Command 5 22 PVCAM Capture Interface Installing 1 15 Q Q Capture Pro 7 overview of features vii system requirements 1 2
65. brate by feature option allow you to calibrate an image by a feature with known size Any line measurement can be used for image calibration Calibration by feature can be done using the following steps 1 Create a measurement of an object of known size For example the length of a bracket can be measured 2 Click the Calibrate by feature length H button the same function can be called from the context menu in the Data Table 3 Fill the feature size in calibration units and other calibration parameters such as Units and Calibration name in the shown Calibrate by feature dialog Calibrate by feature Calibration parameters _ ____ _ J Calibration name Calibration by feature Units Her Millimeter Feature size units 1 000 a Show calibration dialog 4 Optional Use the Show calibration dialog to adjust the calibration measurements 3 20 5 Predictive Calibration The Capture Tab Click Ok to create a new calibration and apply it to the active image Predictive calibration lets you create estimated spatial calibrations based on the optical characteristics of the camera and objectives To use the predictive calibration feature follow these steps 1 Capture Adjust Select Measure View oe me IN Auto Exposure 0 00 FPS Click the Setup button on the Capture tab ths ae 8 sel settings Lens none amp Live Capture None Setup D Ta
66. brightness and intensity range m Use a Region of Interest ROI to examine features ina specific part of the image m Perform analysis using the tint and color composite features vii Conventions Used in this Guide Improve the quality of your images using the background correction and background subraction features m Use filters to enhance obscure features in your image Conventions Used in this Guide The Q Capture Pro 7 User s Guide uses the following documentation conventions 1 Bold Italic Italics Bold quoted text lt KEY gt viii This is a numbered step in a process This indicates the name of a tab or ribbon This indicates the name of a dialog box or dialog box tab This indicates an object on a dialog box such as a text box button spin box list box etc This indicates information that you must type into the system exactly as shown This indicates a key to press in order to perform an action Chapter 1 Installing Q Capture Pro 7 This chapter provides an overview of installation requirements and step by step instructions for installing and activating Q Capture Pro 7 1 1 Installation System Requirements To install and work with Q Capture Pro 7 you will need the following equipment and software Supported Operating Systems Windows XP SP 3 and later 32 bit Windows 7 Professional and Ultimate SP 1 32 amp 64 bit Minimum Requirements Pentium 4
67. cation If you are saving a series of images choose Quick Save as Movie When you select Analysis you will see the following drop down menu A Analysis is Open Saved Images gii Options Quick Save for Analysis stores your images in TIF format so that they can be retrieved for analysis at another time Open Saved Images goes to the Open dialog and lets you select a previously stored image Options takes you to the File Options page where you can change your preferences When you select Publication you will see the following drop down menu lad Publication Auto Open qA Open Saved Images gai Options Publication stores your image in a JPG format that can be easily incorporated into a report or spreadsheet It also burns in any annotations ROIs or measurements and resizes your image The Capture Tab Auto Open will automatically open your saved image in the Q Capture Pro 7 workspace Open Saved Images goes to the Open dialog and lets you select a previously stored image Options takes you to the File Options page where you can change your preferences Viewing Camera and Lens Characteristics The Optical Characteristics group on the Capture tab s ribbon lets you see what dye was used with your captured image Lens F none gai 1 To apply a different lens to your image click the drop down arrow and select an item from the drop down list 2 To edit the lens characteristics click the
68. ce is the interior color This option is useful when working with colored images If this box is not checked no assumption is made about which side of the trace is the interior Use Range Miniumu Range Maximum When checked the range for full scale luminance swings is assumed to be the full minimum maximum range of the entire image If this box is not checked range for full scale luminance swings is Using the Saturation Warning assumed to be the displayed range of image luminance This option is usually left clear Bound Trace by ROI When this box is checked the trace object must stay within the bounds of the ROI If this box is not checked the boundaries of the ROI are ignored Disable Auto Trace When this box is checked auto trace will be disable and you cannot use it until you uncheck the box Using the Saturation Warning Use Saturation to show overexposed and underexposed areas with a given color It can be used with the live preview to set proper exposure time and avoid saturated areas so that an intensity measurements on the image will be correct Threshold 255 E ma Low Level The colors are set using High level and Low level color pickers Intensities that are greater than or equal to the High Level threshold will be colored with the designated high color Intensities that are less than or equal to Low Level threshold will be colored with the designated low color The channels used for saturation test can be
69. d As you move the sliders the image in the workspace displays the changes in intensity Use your cursor to select the color channel that you want to adjust As WJ Brightness Increases decreases all intensity values uniformly In the example below brightness has been increased from the original Original After Brightness Increased Ta y Contrast Increases decreases high and low intensity values reciprocally that is as the high values are increased the corresponding low values are decreased and vice versa In the example below contrast has been increased from the original Original After Contrast Increased T By H Gamma Adjusts the contrast for the high and low intensity ranges simultaneously In the example below gamma has been decreased from the original Enhancing an Image Original After Gamma Decreased Resetting the Image Because the enhancement features Equalize Contrast Enhancement Display Range and Invert Contrast are implemented through a Lookup Table the changes made to the appearance of your images are not permanent You can restore the image to its original appearance at any time To do this Click the Reset Display button G from the Channels group The Lookup Table is reset and the image appears as it did before any enhancement options were defined Making Enhancements Permanent Because the enhancement features Equalize Contrast Enhancement Display Range and Invert
70. dvanced Sening S seinien E O 3 5 Saving Your Captured Images ccseeeeecccceeeseceeeeeeeeeeseeaeeeeeeeeeeeseneaes 3 11 Viewing Camera and Lens Characteristics cccccccssseeeeeeeeeeseneeeeees 3 12 Using Spatial Calibrations cccccccccccccssssseseeeeeesesseeseeeeeeessssaeeseeeeees 3 13 Creating a Spatial Calibration ccccccccccccceseseessseeeeeeessseeeeeeeeeeeeseeeaas 3 14 Saving CalHratonS eei eel auie ithe aunt eleledine 3 22 Applying a Calibration Marker to the Active Image ccccccsseeeeeeees 3 24 INTENSITY Fear S ois A tate A 3 27 Chapter 4 Viewing Intensity Information 11 ccccseeeeneeneneeneeeees 4 1 VIEWING a IMAGE Histogram ccccccccseseecceeeeeeceeeeeeseeeeeseeeeesaeeeeeesaeees 4 2 VIEWING a Line Proe a aitcet eaten net tneal ean N 4 4 Using the Saturation Warning ccccccccssssseceeceeeseeeeeeeeeeeesssaeseeeeeeeees 4 11 Chapter 5 Working With IM QO S cccsssscsessesssnensnsssnenennssseneess 5 1 Openng an MAGS asc sc caudesse abate cane Geist tate ce didcd al a a 5 3 Opening an Recent DOCUMENL ccccccceeeeseeeeeeeeeeeeseseeeeeeeeeeeesaaeas 5 5 See WAT ATS An MAGE amen eee eee ee eee 5 6 USING QUICK SAV C cts coh et oka cease a Seta 5 7 ZOOMAG IMAGE Sena aca e a eee mena a cs Laan 5 9 PAM IMAGES sri E edema tliat 5 11 Enhancing an Image ccccseeccccceseeececeeeeceesececeaseeeseuseessauseeesseeeeesaaeees 5 13 MESIZING an Mage nenn a
71. e Pro 7 There is a newer version of Q Capture Pro F available version 7 0 5 Build 4290 The installed copy of G Capture Pro 7 has not been registered This update may not be downloaded until the installation is registered Press Cancel to abort download of this update Follow the registration prompts in G Capture Pro 7 to complete this process and run Look for Updates again to obtain this update Cancel 3 Optional Click the Details button for more information You will see this message 0 Capture Pra 7 Update Update Details New or Improved Features in G Capture Pro 7 This release includes pre installed capture settings created for your specific Q Imaging camera Significant changes have been made to the Calibration settings The Capture tab features a new Setup group In addition to simphfing the calibration process this group allows you to add a new lens from the Predictive Calibration dialog The Auto Calibration and Zoom features are no longer available The Optical Characteristics group now displays only the lens selection tool The calibration drop down list has been removed from the Characteristics and Calibration groups lt Close Details 4 Click Close Details to proceed 1 31 Installation 5 Once you have registered your software restart Q Capture Pro 7 When a registered copy of Q Capture Pro 7 0 4 is installed you will receive a notice that an update is available You must be running
72. e browse button to choose a saved image to work with Choose a dark or light background to subtract or create a background from a set of points Indicate the maximum feature size in pixels The subtracted background will appear in the workspace 5 31 Enhancing an Image Applying Filters Q Capture Pro 7 filters are used to emphasize features of an image The instructions below demonstrate how to apply the Sharpen filter to accentuate the edges in the castiron tif image 1 Open the castiron tif image in the Q Capture Pro 7 workspace as described earlier in this chapter lt y Fua Gate CAM oo F ps a w Ps Ag to wa D l Ez miy 7 Khe cw Gla tees E T Bio SE 2 Select the Filters group on the Adjust tab s ribbon i 2D Filters 3 Choose the Sharpen filter from the drop down list of available filters 5 32 Enhancing an Image Blur b Enhancement Edge 4 Select Sharpen 50 from the pop out menu Sharpen 50 Sharpen 100 Sharpen edges The filter is automatically applied to the image Advanced Filters The Sharpen and Blur filters are only two of the 2D filters available in Q Capture Pro 7 To see the complete set of Enhancement or Edge filters click the arrow for the pop out menu 2D Filters Pai Edge eer Low Pass Kernel size 3x3 High Pass 5x8 Sharpen Yur Median Strength 100 S Cakes Passes 1 a High Gaussian v Lec Equalization Flatten
73. e index value for each position along the line In a line profile the X axis represents the spatial scale as defined by the spatial calibration and the Y axis measures the intensity value You can display a line profile on both live and captured images To view a line profile 1 Open the image that you want to measure if it isn t open already 2 Select Line Profile from the Capture tab s ribbon A S Line Profile You will see the Line Profile ribbon gt n 6 0 ae ie m K We ees E ee bet PiE ois Sa ES Cuy Tot Prik Bache vend Oneness There are many different tools in the Line Profile ribbon The drawing tools are similar to the ROI and Annotation tools xX aK Width 1 Tools Viewing a Line Profile When you select the Line Profile Q Capture Pro 7 will automatically draw a single line on your image re E This profile is labeled Line 1 The Line Profile graph appears in a pane below the image 300 Line 04 380 200 10 OS S 4 o o w w 10 w b w o a o A a o o o a an E kz Ye hh kk 37 i AR As you draw more lines on the image they will also appear in the profile pane Viewing a Line Profile Line Profile Tools The Line Profile ribbon includes many useful toolbars Copy Tools Copy Save N i ia a Print Copy Use the Copy button to copy graphs or tables to the Windows Clipboard You can also export the data to o
74. e of the application window tages be mas ie ae rn i i Eaka a heer he Vee ae dimen LOE e a D Pe one a on egde es i tie a ee EnA a Ames Sa amann deine Punirsten Image Strip 9 6 Customizing the Image Strip If you have several images open the Image Strip will display thumbnails of all of them Image Strip ax The layout of the images can be selected from the Context menu Click the right mouse button on the image thumbnail to see the Context menu To change how the thumbnails are displayed in the Image Strip choose one of the options from the Thumbnail size pop out menu peal E X5 L 5 l Activate Close T a Auto Fill Single Row Column Fixed Small Fixed Medium Fixed Large 9 7 Customizing the Image Strip Auto Fill all thumbnails are fitted to the strip window lim e Strip 1 x ls eet ll I L Mia Single Row Column when this mode is activated the thumbnails are resized to fit the width or height of the window Scrollbars become visible if the number of images exceeds the size of the window ewe t oy ewes ort A La AA Terk A vir poet A Siete sd Shia Axe ty ee z 7 aiai a EA ag r na a oF ASS a e mE Fixed Small thumbnails are 64x64 pixels m Fixed Medium thumbnails are 128x128 pixels m Fixed Large thumbnails are 256x256 pixels 9 8 Customizing the Image Strip
75. e screen i Q Capture Pro 7 Setup Welcome to the Q Capture Pro 7 Setup Q CAPTURE 7 The setup will install Q Capture Pro 7 on your computer Click Next to continue or Cancel to exit 3 Click Next Q Capture Pro 7 requires the Microsoft NET framework If this is not installed on your computer the application will install it automatically You will see the Q Capture Pro 7 License Agreement i Q Capture Pro 7 Setup End User License Agreement Please read the following license agreement carefully IMPORTANT PLEASE READ CAREFULLY LICENSE AGREEMENT THIS LICENSE AGREEMENT AGREEMENT IS BETWEEN YOU THE END USER AND MEDIA CYBERNETICS INC MEDIA IT GOVERNS THE USE OF THE SOFTWARE PROGRAM MEDIA AND DOCUMENTATION KNOWN AS QO Capture Pro 7 THE PRODUCT IF YOU USE THE PRODUCT THEN YOU AGREE TO THE TERMS OF THIS AGREEMENT IF YOU ARE NOT WILLING TO BE BOUND BY THE TERMS OF THIS AGREEMENT w mm ee ee accept the terms of this License Agreement Back Next 1 4 Installation 4 Check the box to accept the license agreement and click Next The Select Destination Folder screen appears iy Q Capture Pro 7 Setup Destination Folder Where and how would you like G Capture Pro to be installed Install G Capture Pro 7 to C Program Files Qlmaging Q Capture Pro 7 Install Application for Current User All Users 5 Indicate the program location a
76. e until you click the triangle again Image Strip The Image Strip appears in the Overview panel along the left side of the Q Capture Pro 7 user interface It displays thumbnails of the images that you currently have open as shown here Image Strip Rx Right clicking on any thumbnail in the Image Strip displays a pop out menu 2 21 The Q Capture Pro 7 Application Window le Activate ea Close lad Save for Publication Save for Analysis Lock options Eg Thumbnail size From this menu you can activate view the image save or close the image zoom or pan on the image or change the size of the thumbnail 2 22 Chapter 3 Captunng Images This chapter provides basic instructions for setting up and capturing images from a camera attached to your computer 3 1 The Capture Tab The Capture Tab 3 2 The Capture tab dictates how images are brought into the application for viewing manipulation and analysis The Capture tab s ribbon contains groups for camera control The functions described in this chapter are located on the Capture tab s ribbon VS Capture Adjust Select Measure View E7 AJ Auto Exposure 0 00 FPS 4 a P Of a s e m e es Goo Live Capture None Setup x Fa Sa PaA oh Saturatior Analysis Publication Movie Cameras Setup i Optical Characteristics Calibration Intensity Quick Save Using a Digital Camera The instructions in this
77. ected Delete All Use the buttons to remove one or more of the annotations from your image Group Ungroup Use these buttons to collect your annotations into a group or to ungroup them Order Use these buttons to order your annotations front to back or back to front Cut Copy Paste Use these tools to cut copy or paste annotations Burn In Use this button to make the current annotation a permanent part of the image Display Options Use this button to see the Display Options panel 6 5 Creating an Annotation Creating a Text Annotation To create a text annotation 1 Choose the Text tool Al in the Annotate group Mala 4 x ax 2 ss oS tay T _ Burnin Annotate 2 Click and drag the mouse in the image to draw a bounding box for the text When you release the mouse the Text tab of the Display Options dialog box is displayed Tet m ejjel a sil Visible E Width 1 al Style TR kj Visible s Otons ______ V Zoom with Image Opacity OH Layer Layer 0 l Start Frame 1 a End Frame 1 j Saveas Default j 3 Type the desired text in the Text tab s field 4 Q Capture Pro 7 will apply your text automatically Arranging Annotations The specified text appears as an overlay in the image For instructions on changing the font color or bounding box attributes of the text annotation please refer to
78. ellipse type ROI in the active image If one doesn t exist draw one 3 Click the Display Options button E in the ROI group You will see the Display Options panel along the right side of the workspace Ellipse Rectangle Polygon Label Border 4 Visible oO a Width 1 E Angle C 0 Fe Style yj j F Visible O Toptom Zoom with Image Opacity _ _U Layer Layer 0 Start Frame End Frame 1 Save as Default j 4 Click Save as Default The default appearance values are set Future ROIs of that type will appear as the selected ROI 7 12 Chapter 8 Measunng Objects in Images This chapter describes how to measure features in images 8 1 Ove rview of the Measurement Feature Overview of the Measurement Feature 8 2 Q Capture Pro provides tools for measuring features in images For example suppose you want to know the distance between two points in an image Using the Measurement tools you can simply draw a line between the two points and create an instant distance measurement All of these tools are found on the Measure tab s ribbon im pi E E qa E o a Image Tools Capture Adjust Select Measure View Image a Data Apply WX Table ji E Measurements Class Measurement operations in Q Capture Pro 7 are performed in terms of image pixel positions For examp
79. entation are provided with Restricted Rights Use duplication or disclosure by the U S Government is subject to restrictions as set forth in the Rights in Technical Data and Computer Software Clause at DFARS 2952 227 7013 or subparagraphs c 1 and 2 of the Commercial Computer Software Restricted Rights at 48 CFR o2 227 1L9 as applicable Contractor Manufacturer is Media Cybernetics Inc 401 N Washington Sts Surte 350 Rockville Maryland 20850 EXPORT CONTROLS You agree not to export or re export the PRODUCT directly or indirectly to any countries end users or for any end uses that are restricted by U S export laws and regulations without first obtaining permission to do so as required by the U S Department of Commerce s Bureau of Industry and Security or other appropriate government agency These restrictions change from time to time If you have any questions regarding your obligations under U S export regulations you should contact the Bureau of Industry and Security U S Department of Commerce Exporter Counseling Division Washington D C 202 482 4811 http www bis doc gov GENERAL T You may not sublicense assign or transfer the license or the PRODUCT in whole or in part except as expressly provided in this Agreement Any attempt to do so is null and void Zis This Agreement will be governed by and construed in accordance with the law of the State of Maryland without regard to the conflict of
80. ered Please register today using the license registration form Click OK This will load the registration web page in the built in web browser 1 21 Installation E Qluaging Product Registration Registered users receive information regarding technical support product fixes and updates and upgrade information The information you provide will also be used to help improve technical support upgrade development and new product development Qlmaging does NOT sell or market the information you provide NOTE indicates required fields Primary Contact Information Title Dr OMr OMs OMrs OProf First Name Last Name Job Title Organization Address City Postal Zip Code Region Select from list Phone Fax Email Product Information Select product category chose at least one type of product E Camera Product Name QICam Mono Uncooled Serial Number E Software oduc l oftware Product QCapture Pro T Name Unlocking Code Serial Number Platform 3 Enter the Unlocking Code and Serial Number s that were sent with the product together with standard registration information 4 Click Submit Once your information is received the license file will be installed on your machine automatically 1 22 Installation Finding and Receiving Updates Q Capture Pro 7 uses the TrueUpdate feature to ensure that you are running the most current version of the software TrueUpdate i
81. et the number of frames to caputre and the time interval using the spin buttons or slider Burst frames allows you to capture a number of images very quickly Check Enable Movie Acquire if you want to capture a series of images as an AVI or sequence file When the Movie Acquire selection is on on the Capture button on the ribbon will change to Movie The Total Duration reports the total duration of the time lapse acquisition Expand Capture Region to set the boundaries for acquiring Regions of Interest as shown here x Or O g SetROL Width 426 Height 379 G Rese Full Size 426 x 379 l emeas d _ ee The X and Y coordinates indicate the position of the RO on your image The Width and Height indicate the size in pixels of your ROI To create an ROI interactively click Set ROI The live preview will be started on the active camera if a preview is not already on and a red rectangle overlay will be centered on the preview image You may then resize or move the ROI to the area desired The button changes to Save ROI Click the Save ROI button to apply the new region coordinates The live preview will then show the region selected The Reset button erases the ROI and resets the area to full frame Click the Locked button to make the ROI settings equal for both preview and acquisition 3 7 The Capture Tab 6 To adjust the image color expand the Color Controls as shown here F ped 4 1 00 Ss
82. eters Optional Check the Show Calibration Dialog box if you want to edit the calibration after you ve created it Click OK to create the predictive calibration Clicking the OK button will generated multiple calibrations for the list of selected lenses and zooms for the given camera If multiple zooms are present the calibration with zoom closest to 1 will be used as default lens calibration The list of camera manufacturers and camera models is loaded from Camera list cfg file in the Resources folder of the application Saving Calibrations Calibration definitions are saved per session whenever they are applied to an image If you want calibration definitions to be saved between sessions however you must save them to a file Once a calibration definition is saved to a file you can load it whenever it is needed in the future To save calibration definitions 3 22 Click the Save Calibration button q3 on the calibration dialog The Save Spatial Calibration list box appears If an active image is displayed in the Q Capture Pro 7 workspace that has a calibration definition assigned to it that calibration definition appears in the Spatial Calibration dialog box From the pull down list box at the top of the Spatial Calibration dialog box select the calibration definition you want to save 5 The Capture Tab Click Save from the Spatial Calibration group TIP If you want to save all calibrations listed in t
83. f a Add to Color Composite Dye Custom Emission Wavelength umi Tint Color Hue E y Apply Dye Tint to Image Check the Tint Image On Off box to show or hide the tint on your image 5 19 Enhancing an Image Check Add to Color Composite to automatically add your tinted image to the color composite image The Dye drop down list displays a list of your current set of favorite dyes You may select a different dye from the drop down list and all the remaining controls will be updated to show the characteristics of the new dye If the dyes you want are not in the current set click the settings button to display the Dye Editor dialog where you can view a complete list of the available dyes Here you can add select a new favorite dye add a dye to the list or delete old ones The Emission Wavelength drop down displays the emission wavelength for the dye you have chosen You can adjust the emission wavelength by clicking on the spectrum or entering a new value The default Tint Color will change in response to changes in the emission wavelength The Tint Color box lets you choose a color to indicate a particular dye Here you can change the color associated with the selected dye or define a custom color The default color is linked to the emission wavelength When you change the emission wavelength the tint color and hue change The default Tint Color can be adjusted to a different hue by using the Hue slider or by enterin
84. f zoom presets or to put the image in fit to window mode so that whenever you resize the image window the image is enlarged or reduced to the size of the window For example you can zoom the image to a specific percentage of its actual size such as 50 or 200 5 10 Panning Images 1 Position the cursor over the image and right click the mouse You will see the scale bar 100 U 2 Move the slider to the right to increase the size of the image or to the left to decrease its size You may also click the arrow to see the drop down list of zoom presets The scale bar also appears in the status bar at the bottom of the Q Capture Pro 7 workspace Panning Images The Pan tool is used to position an image that does not fit entirely within the image window You can m Use the Pan button in the Zoom group m Pan in the Image Strip Panning Using the Pan Button 1 Click the Image tab from the Q Capture Pro 7 menu bar The Zoom group is displayed a pad room Par Local Lock Zoom Zoom 2 Click on the Pan button 3 Use your mouse to move the image around in the frame Panning Images Panning in the Image Strip Using Image Navigator The Image Strip displays a thumbnail of your entire image When the active image is zoomed and doesn t fit in the viewer the viewing area is shown on the image strip thumbnail as a semitransparent overlay 9 e 5 sd E p A EF oe Bree 4
85. for ROls 7 3 Selection tool for annotations 6 3 Sequence toolbar 5 34 5 35 Set Order 6 5 Settings File 3 10 Sharpen filter 5 32 Shortcuts 9 3 9 5 Show Measurements 8 11 Show Statistics 8 11 Software license 1 20 Software Registration 1 20 Spatial Calibration 3 14 3 16 3 18 3 19 3 22 3 23 Spatial Calibration group 3 14 3 16 Status bar 2 13 2 16 Status bar of Q Capture Pro 7 described 2 16 Status Bar Options 2 13 Subsample 5 36 Subsample Sequence Dialog Box 5 36 System requirements 1 2 T Tagged Image File Format 10 2 Text annotations 6 6 Text tool 6 3 6 6 The Image ContextMenu 5 8 Thumbnails 9 7 Time Lapse Controls 3 7 Toolbars overview of customization options 9 6 Tooltips 5 16 9 9 Transform group 5 13 5 15 Index 6 TrueUpdate 1 23 U Units Spatial Calibration 3 17 Units of measure 8 8 Updating Your Product 1 20 User Interface Customizing 9 1 V Version 7 0 4 1 20 1 25 1 27 1 31 1 32 Version 7 0 5 1 20 1 24 1 25 1 30 Video tutorials 2 1 View tab 2 19 W Welcome screen 2 1 2 2 5 1 White Balance 3 4 3 8 Windows XP 1 3 X XOR pixels 7 5 7 7 Z Zoom and pan controls 5 9 Zoom Button 5 9 5 11 Zooming images 5 9 www QIMAGING com tel 1 800 874 9789 support qimaging com 2011 Qlmaging All rights reserved This program is protected by US and International copyright laws as described in the Help and About sections O
86. g an ROI be sure the image you want to work with is opened in the Q Capture Pro 7 workspace To define an ROI in the active image 1 Goto the ROI no on the Select tab s ribbon Regions of Interest 2 Click on the ROI tool you want to use and draw the ROI in the image as described for that tool below Selection Tool Use the arrow to select an ROI on the image Click the drop down arrow and use the Multiple ROI tool to select all the ROIS in an image Rectangle or Rounded Rectangle ROI This tool allows you to define a rectangular or square ROI Click and drag the mouse from one corner of the Region of Interest to the opposite corner To force a perfect square hold down the lt Shift gt key as you click and drag the mouse Circle or Ellipse This tool allows you to define a aa oO circular or elliptical ROI in the image To force a perfect circle hold down the lt Shift gt key as you click and drag the mouse Drag to here and release Defining an RO i IN Rectangle by Numbers This tool allows you to define a rectangular RO by entering the left right top and bottom locations E Mew ROI by Numbers Lett Top Right Bottom Polygon This tool allows you define a polygon shaped or freeform ROI in the image To define a freeform ROI Use this technique to create smooth edges Hold the left mouse down while you draw with the cursor to create the desired freeform shape Double click
87. g an new Hue value A completely custom Tint Color can be selected by clicking the down arrow next to the Tint Color box and selecting a standard or custom color The Emission Wavelength and Hue override a custom Tint Color so to set a custom color you should first set the wavelength and then set the color using either the Hue controls or the Tint Color Click Apply to add your dye or tint color to the active image Using Color Composite Use Color Composite to create and configure color composites using black and white source images You can access the Color Composite dialog box through the Color group on the Adjust tab You can combine grayscale images into a color composite Any group of grayscale images that are of the same size can be mixed in a color composite Images of 8 12 16 bit integer or floating point format are combined into a 24 bit color composite Each input channel will have individual LUT adjustments as well as a registration offset to line it up with the rest of the images You can also combine individual channels from a single image Each channel will be listed separately To create a color composite follow the steps below 5 20 Enhancing an Image 1 Open the images that you want to combine In this example we are using DemoRed tif DemoBlue tif and DemoGreen tif 2 Click the drop down arrow in the Composite area in the Color Group on the Adjust tab Cpe 3 Select Add All from the drop down men
88. g the Load button Specify the number of distinct colors you want to associate with the selected intensity range The number of colors is selected using the Divisions spin buttons Indicate the number of spins If you want the spectrum to repeat over the intensity range of the image you can set the Spins to the number of times to repeat the spectrum Save the settings for future use with the current image or with another This step is optional You do not need to save the pseudo color assignments in order for them to be applied to the active image If however you want to save it for continuing use or to exchange with a colleague you need to save it When you click the Save button Q Capture Pro 7 saves the current pseudo color palette settings Enhancing an Image Adjusting Color Channels To adjust the color channels or load a saved Look Up Table LUT use the Channels group in the Display group amp __o 50 Eee 21 a m Rz amp _ _3 _ 0 S WL 242 Fel wt Q a ey Y amp _24 e 1 a An B Ey i td Best Fit s u Display The Channels feature contains the following tools Apply Display to i Select Channel Image ny va Invert Display Reset Display J Load LUT Save LUT 1 Click the drop down menu arrow 2 Select the channel you want to adjust The default is All channels All FE bad Green Channel i d Blue Channa Acvanoed 9 Click
89. ge based on the maximum feature size and dark polarity of the active image Bright Background Choose this setting to create a background image based the on maximum feature size and bright polarity of the active image Background From Points Choose this setting to create a background image based on a set of points on an image Background Correction should be used for optical density applications as it accounts for the fact that optical density is not a linear function of the gray scale You have the following choices Dark Background Choose this setting to create a background image based on the maximum feature size and dark polarity of the active image Bright Background Choose this setting to create a background image based the on maximum feature size and bright polarity of the active image 5 30 Enhancing an Image Background From Points Choose this setting to create a background image based on a set of points on an image The Options allow you to create a new image from the background image Create New Image Extract background image displays the background image during black and white operations To use the background commands follow these steps 1 2 Open the image that you want to correct Click the Subtract drop down arrow You will see the following dialog Dark Background Bright Background Background From Points Feature Size ree rasa NUCSTAIN TIF y Browse You may use th
90. hange the color of the text Zoom with Image Check this box to make the annotate larger or smaller as the image is resized Setting the Appearance of ROIs Label Properties Some common controls available for changing the appearance of label annotations are described below Tet Mew Label Arial g 15 Ej PB ee ES E Text Click on the Text tab to change the text of the annotation Font feist Allows you to change the font to be used for rendering text Size 20 a Allows you to change the font size to be used for rendering text B Ue lt Allows you to make the text bold italics underlined or strike though a letter 5 When you have made the desired changes click the ROI Display Options panel The changes are applied to the selected ROI 7 11 Setting the Appearance of ROIs Setting the Default Appearance for ROIs The default appearance attributes for ROIs are set by designating the appearance attributes of an existing ROI as the default for future ROIs of that type Note that defaults exist independently for each type of ROI you can draw rectangle rounded rectangle ellipse polygon point and text To set the default appearance for an ROI type 1 Click the Selection tool from the ROI group 2 In the active image click on an ROI of the type for which you want to set appearance defaults For example if you want to set the default appearance for ellipse type ROIs click on an
91. hat you want to open Use the Look in pull down list box to locate the folder in which it is stored 5 3 Opening an Image ts UPC LRG TIF 4 Click the Open button The selected image is opened in the Q Capture Pro 7 workspace T x E aT mpm T s D a Pat i s Opening an Image Opening an Recent Document To open an image or document that has been recently opened in Q Capture Pro 7 Application button 1 Click the Application button Capture Adjust Select Measure 2 Select an image from the list under Recent Documents The image will appear in the Q Capture Pro 7 Workspace You can use the push pin icon to pin a recent document to keep it in the list Q bao ke Geom Image Too Recent Documents 1 NUCSTAIN Image file name Options kg Exit Q Capture Pro 7 5 5 Opening an Image Saving an Image After you have finished working with your image you will probably want to save it To save an image follow the steps below 1 Click the Application button The pull down application menu appears Q bdo k oox Image Too Recent Documerts 1 NUCSTAIN TIF Options kj Exit Q CapturePro 7 2 Choose Save You have a choice of saving only the active document image or saving all modified documents or images Your image is saved automatically Opening an Image Using Quick Save In addition to using the Save to File feature of the image setti
92. he Spatial Calibrations list box skip Step 2 and click on the Save All from the Spatial Calibration group The Save Calibration dialog box appears Save calibration Savein O Images I ColorMerge 4 ia LineProfile My Recent Micrometer Documents G Desktop My Documents My Computer My Network l Flaces File name Save as type 1 Calibration files 10C w Canca In the File Name text box type the name you want to give to the current calibration definition or set of calibration definitions being saved Click on the Save button 3 23 The Capture Tab Applying a Calibration Marker to the Active Image The calibration marker is like a distance key on a map it shows a line and expresses what that line represents in terms of meaningful units In Q Capture Pro 7 it is composed of two or three overlays as shown below e de E T When you add a calibration marker Q Capture Pro 7 adds it to the currently active image based on the number of pixels in the image Do o i Background optional r Marker To set the calibration marker to appear in the active image 1 Choose the Auto Marker button from the Calibration Marker drop down A marker will be placed on all open and active images automatically SS in the 2 Otherwise click the Add Marker Bar button Q Capture Pro 7 toolbar A calibration marker will appear in the image
93. he desired destination Delete Selected Use this button to remove one line profile feature from your image Delete All Use this button to remove all the line profile features from your image 4 7 Viewing a Line Profile Profile Tools Show or Hide Profile La Name Line 1 Opacity 50 He ed Right Profile The Profile tools control the appearance of the line profile features and graph You can show or hide the line profile label adjust the opacity of the line and change the colors that appear on the graph The Left and Right arrows move the selected feature to the left or the right Background Tools None Backoround The Background combo box is used to select a background profile values of background profile will be subtracted from all other profiles values Background smoothing is used to select the type of smoothing for background profile values The Points box indicates the number of points visible on the graph 4 8 Viewing a Line Profile The Options group contains controls for displaying the image f Mono Interpretation Ml Image Strip Update NormahizeBackoround Spatial Calibration g Back round Wise Chie ols cy PR ition Options o E The Log group displays the X and Y axis in a logarithmic scale Close Line Profile To exit the Line Profile ribbon click the Close button When you close the Line Profile ribbon the Line Profile panel is no longer displayed
94. his dialog on open Check this box to see the Welcome screen each time that you start O Capture Pro 7 Close Click this button to close the Welcome screen The Q Capture Pro 7 Application Window When you exit the Welcome screen you will see the application window When it is first opened the application window consists of a menu bar and tabbed ribbons across the top of the application area and a status bar at the bottom If you have an image open the image is centered in the Q Capture Pro 7 workspace Tabs Function Ribbon Bar J e TES laps fn T SCS TACIT g ri Caie Aa A kissie Vei E ss ar we Wm aa M D l a lE re a Aa lie Eh inane Jew mmm iE UME itoten Ans Publication tack tava Application button Detti raritete i abbr inay Quick Access toolbar Function Groups ahja bateraga pong pae DID A j BE jea RO a jana dtd Status Bar Image Strip The center of the window contains a set of panels or panes The far left panel is the Overview panel which contains the image strip and tools that provide an overview of the workspace contents The center panel contains the active content which can be an image sequence or other rendering The far right Details panel contains additional dialogs that enable you to modify the contents of the workspace An alternate details panel below the center panel contains other useful information such as a table or graph a Jepa Raph Agna loi tidie
95. icenses The workstations must be owned leased or otherwise controlled by you whether in a network or other configuration 2 Create a quantity of backup copies of the PRODUCT in any machine readable or printed form equal to the number of PRODUCT licenses you have purchased 3 Transfer the PRODUCT and your rights under this Agreement to another party if the other party agrees to accept the terms and conditions of this Agreement If you transfer the PRODUCT you must at the same time either transfer all copies of PRODUCT to the same party or destroy any copies iii not transferred You must immediately notify Media of the transfer 4 Print out one copy of the Product documentation from the program media for each license purchased If you print out any part of the Product documentation from the program media you must reproduce and include all the copyright notices that appear in the documentation on any such copy of the documentation YOU May nort 1 Use or copy the PRODUCT in whole or in part except as expressly provided in this Agreement 2 Use the PRODUCT concurrently on more than the number of workstations for which you have purchased licenses 3 Copy rent distribute sell license or sublicense or otherwise transfer the PRODUCT or this license in whole or in part to another party except as specifically set forth above 4 Incorporate the PRODUCT or any portion of the PRODUCT into or use the PRODUCT or any
96. ick the mouse Click and drag a rectangle around the area of interest on which you want to zoom The image is magnified If needed you can click or drag the mouse again to magnify the area of interest even further 5 9 Zooming Images Zooming with Local Zoom The Local Zoom feature provides a tool to show a zoomed area of an image in a separate window with a given zoom factor It can be used with manual measurements the calibration marker manual tagging etc When you select Local Zoom a new window opens on the right side of the application area Local Zoom 1 x Zoom 400 Crosshair fj The cross hairs in the Local Zoom window shows the current cursor location This makes placing accurate manual measurements very easy even on large images Zoom Choose the required zoom factor from the drop down list Cross hairs Checking this box turns the cross hairs on un checking the box turns the cross hairs off The color picker button selects the color of the cross hairs Zooming by Using the Mouse Wheel If your mouse has a wheel on top between the left and right mouse buttons you can use this wheel to zoom in and out on the image Simply roll the wheel while the image is active in the Q Capture Pro 7 workspace Roll the wheel toward you to zoom out on the image and roll the wheel away from you to zoom in on the image Zooming by Using the Scale Bar This method for zooming allows you to take advantage o
97. ick the Back button to return to the Select Target Application dialog to ensure that you are choosing the correct product installation Then click Next to continue Note If your system is configured with multiple Olmaging software products please make sure to upgrade all software installations with this version 7 0 of the OImaging Capture Interface If you choose not to update uncheck an existing installation that product may not be able to use your camera after updating to the current QCam driver version 2 0 13 This lack of backward compatibility is apparent when using the QCam driver version 2 0 13 and higher with a Olmaging Capture Interface in versions earlier than 7 0 Installation The Select Destination Folder screen appears Destination Folder s Where would you like Glmaging Capture Interface to be installed Install Qimaging Capture Interface to Image Pro Plus 7 0 Install to CIPWIN7O0 Image Fro Insight 8 0 Install to C Program Files amp 86 Media Cybemetics Image Pro Insight Image Pro Premier 9 1 Install to C Program Files amp 86 Media Cybemetics Image Pro Premier 9 1 QCapture Pro 7 0 Install to C Program Files amp 86 Qimaging G Capture Pro 7 5 Indicate the program location then click the Next button The Ready to Install dialog box appears Ready to install Qimaging Capture Interface Click Install to begin the installation Click Back to review or change any of your
98. installation settings Click Cancel to exit QCam Driver version 2 0 9 8 or later is required to ensure corect operation This screen indicates that your computer is ready to install the O maging capture interface 1 12 Installation 6 Click Next to continue The nstalling dialog box appears 74 Olmaaing Capture Interface 9 Se bey maging ap Installing Qlmaging Capture Interface 9 Please wait while Qlmaging Capture Interface 9is installed 7 The setup program will begin copying and installing files Click Next when the installation is complete The next page shows that the capture interface installation is complete eT Completed the Qimaging Capture Interface installation Click the Finish button to ext E Canca 8 Click the Finish button 1 13 Installation You may see the following message Oo Warning QCam Driver Required b i Before you can use your camera you must install the QCam Driver This installer can be found on the installation disk provided with your camera and can also be downloaded from the QImaging support Web site If you have not yet installed these files you must do this before you will be able to use your camera Would you like to visit the QImaging Web site now bia ee 9 Click Yes to go to the Q maging web page 1 14 GITAL IKAGING MADE EASY SUPPORT B Glmasing Seftwave Downlaads FAQs d download the maal curent siame he your
99. iple ROIs LEi button in the ROI group so that it is highlighted This is the default selection When this tool is selected you can define multiple ROIs in a single image Arranging ROIs After you have defined an ROI you can move it resize it and delete it Moving an ROI To move an ROI 1 Click the Selection tool CRI from the ROI group 2 Click on the ROI you want to move The ROI is selected 3 Position the cursor over the selected ROI If the cursor is positioned correctly the Dragging tool ah appears 4 Click and drag the ROI to the desired location Resizing an ROI ROIs can be resized by moving their control points To resize an ROI 1 Click the Selection tool CRI from the ROI group 2 Click on the ROI you want to resize The selected ROI is selected 3 Position the cursor over the control point that you want to move Arranging ROIs If the cursor is positioned correctly the Resize tool f or appears over the control point 4 Click and drag the control point to the desired location Using XOR Pixels Use this feature to examine pixels in overlapping ROIs on your image To apply a filter using XOR pixels follow these steps 1 Draw two or more ROIs on your image as shown here gt E i 2 4 p i ry 2 Goto the Adjust tab and select the Filters group 3 Choose a filter from the filters list gt Low Pass Kernel size 3x3 Hioh Pass 55 Sharpen JTT ra O Meda
100. justs the value for the X spin box to match the entered Aspect Ratio value If there is a feature pictured in the image that represents a perfect square 1 Click the From Image button A in the Aspect Ratio area A dialog box appears in the Q Capture Pro 7 workspace and a diagonal annotation line appears in the image 2 Click and drag the line over the reference object and then adjust the line s control points so that the beginning of the line corresponds with the bottom left corner of the reference object and the end of the line corresponds with the top right corner of the reference object TIP Again for greatest accuracy it is often helpful to zoom in on the reference object 3 Click OK The Spatial Calibration dialog box is redisplayed and the calculated aspect ratio value appears in the Aspect Ratio spin box Additionally the X and Y spin box values are automatically adjusted to account for the aspect ratio The Capture Tab ay 10 Click Apply to Active Image omen The spatial calibration is applied and saved for the session To make it available across sessions you must save it to a file see Saving Calibrations below 11 Click Apply to Active Image 7 in the spatial calibration toolbar The spatial calibration is applied and saved for the session To make it available across sessions you must save it to a file see Saving Calibrations below Calibrating by Feature Cali
101. le oS Style Fi Visible r5 Ee Optiors Zoom with Image Opacity e _____ _ 8 P zi o Layer Layer 0 Start Frame ja 1 1 Saveas Default i End Frame Click Save as Default The default appearance values are set Future annotations of that type will appear as the selected annotation Note that when you exit Q Capture Pro 7 your appearance values are reset to the defaults 6 13 Setting the Appearance of Annotations Saving Annotations When annotations are originally placed on an image they exist as graphical overlays and are not part of the image If you want them to become part of the image however you can save them with the image 1 Click Save from the Application menu Q a ke S 0x Image Too lew s Recent Documents 1 NUCSTAIN TIF Options kj Exit Q CapturePro 7 Your image and annotations will be saved automatically a Burn in You can also use the Burn In button to make the annotation a permanent part of the image Using this option does alter the pixel data For that reason we recommend creating a copy of the original image and save it without the altered pixed data Note Annotations can be saved with TIF TIFF and SEQ format images 6 14 Chapter 7 Using ROIs You can define one or more Regions of Interest ROIs in a variety of shapes and you can control the positioning size appearance a
102. le the length of a line feature is determined by the number of pixels along the line Pixel level measurements however can be converted to more meaningful units such as inches or millimeters using spatial calibrations Refer to the section about Spatial Calibrations in Chapter 3 for more information Making Manual Measurements Making Manual Measurements This section describes how to define measurements and view your measurement data Measuring Features in an Image Q Capture Pro includes line measurement and manual tagging tools Line tool Selection tool A i Delete selected line me Manual Tag tool Show Data Table Delete all Measureme Measurement display options 8 3 Making Manual Measurements 8 4 To make line measurements in an image 1 Open the image that has features you want to measure The image appears in the Q Capture Pro 7 workspace Gg S F i faye i aS E o ET m i Ft o vee lage ktl t F iiia lp hA Da raum 7 a y LT r a i j 2 2 Pas ima mee CA ks a al a ee dies a pase kone Ae iT agir oe i Rope iig n F Fae ee 2 ae wte ae m 2 Go to the Measurements group on the Measure tab s ribbon a FEE on _ e ey z DEA ms L bi 1 LG i Measurements Select the line tool mne l This tool allows you to measure a line drawn on the image Apply or define the spatial calibration that you want to use See Managing
103. n Strength Li 1 on Passes 1 _ Gaussian High Gaussian Loc Equalization Flatten Rank Despeckle 4 Click Apply Arranging ROIs Your filter will be applied to the area between the flags on the image Rotating an ROI You can rotate a selected ROI without rotating the image 1 Click the Selection tool A from the ROI group 2 Click on the ROI you want to resize The selected ROI is selected 3 Position the cursor over the center point in the ROI that you want to rotate You will see the rotation cursor 4 Click and drag to rotate the ROI Deleting an ROI To delete an ROI 1 Click the Selection tool RI from the ROI group 2 Click on the ROI you want to delete 3 Click the Delete es button or Press the lt Delete gt key Note that you can also use the delete button in the image context menu click the right mouse button on the image when an ROI is selected to delete the selected ROI TIP You can use the Delete All tool in the ROI group to instantly remove all ROIs defined in the active image Setting the Appearance of ROIs Setting the Appearance of ROIs These instructions explain how to change the appearance of an existing ROI and how to set the default appearance attributes for the different ROI types Changing the Appearance of an ROI Sometimes you might want to change the border or fill color or size of your ROI to make it easier to see on your image To change the appearance of an R
104. n or polyline will start the auto tracing If uncheck start auto trace by holding down the lt Ctrl gt key and clicking where you want the trace to start The lightening bolt cursor shows you the auto trace path AutoAccept Final Trace When this box is checked the auto trace automatically returns to the starting point to finish tracing the object If unchecked you must press the lt Enter gt key or double click the mouse to place the final point and complete the auto trace Trim Leading Points Frequently a trace will not return to its starting point but will interest with itself some distance from the starting point If this box is checked the points before the intersection point are removed if the intersection is close to the starting point If this box is not checked you must edit the trace manually to remove extraneous points Remove Redundant Points If this box is checked all horizontal vertical and diagonal runs leading to the auto trace are replaced by their end vertices in the final result If this box is not checked these points will be retained Monochrome Interpretation When this box is checked image color is ignored for edge detection only average luminance is used If unchecked each color channel s luminance gradient is considered when doing edge tracking Assume Clockwise When this box is checked the trace will begin moving clockwise around the object This information is used to determine which side of the tra
105. nd display status of ROIs This chapter provides step by step instructions for defining and working with ROIs to isolate areas of interest in your images You can find the ROI feature on the Select tab s ribbon Q Fbad E FH Oo lt Image Tools i Capture Adjust Select Measure View Image a io ha ae meri d a ti a H kins 7 r aY 1 oo r W i dk G em Hee amp Ai Ge Na T ll Copy Noe ki i Hi iors Burn Ir el E Crop Ha mJ To i hi Clipboard Regions of Interest Annotate What is an ROI What is an ROI An ROL is an Region of Interest that is isolated from the rest of the image Certain Q Capture Pro 7 commands can be constrained by an ROI Once you have defined an ROI the command applies to only the pixels within the ROI Examples of commands that are constrained by an ROI are Save As Cut Copy Duplicate Rotate and Resize For example in Figure 7 1 below a square shaped ROI has been drawn around a particular object of interest in the image When the Duplicate command is applied to the image the ROI portion of the image is copied to a new image as shown in Figure 7 2 Figure 7 1 Figure 7 2 You can also apply a filter to an ROI Figure 7 3 show an image with an ROI drawn on it and a Sharpen filter applied to the selected area Figure 7 3 More information about applying filters appears in Chapter 5 Defining an ROI Defining an ROI Before definin
106. nd how many users will be using it then click the Next button The Ready to Install dialog box appears i Q Capture Pro 7 Setup Ready to install Q Capture Pro 7 Click Install to begin the installation Click Back to review or change any of your installation settings Click Cancel to exit tae This screen indicates that your computer is ready to install Q Capture Pro 7 6 Click Next to continue Installation 7 The Jnstalling dialog box appears ie Q Capture Pro 7 Setup Installing Q Capture Pro 7 Please wait while Q Capture Pro 7 is installed Status Removing backup files 8 The setup program will begin copying and installing files Click Next when the installation is complete Note During the installation process you may see the following warning message Sentinel HASP Run time Environment Installer v 5 86 Newer version of the HASP SRM Run time Environment already installed Only Vendor Libraries and Provisional Products will be installed This warning can be safely ignored and installation of Q Capture Pro 7 will complete successfully 1 6 Installation The final installation screen indicates that O Capture Pro 7 is now installed ie Q Capture Pro 7 Setup Completed the Q Capture Pro 7 Installation CAPTURE Click the Finish button to exit The application portion of setup is complete Please press Finish below after which installation of the Qimaging capture interface will
107. ng installed Q Capture Pro 7 The next dialog indicates that the installation is complete 1 33 Installation b Please press OK to complete installation of the Q Capture Pro 7 0 5 aL patch If a reboot was indicated as necessary by the patch please remember to do so before using Q Capture Pro 7 0 5 11 Click OK to begin using the updated product You may need to restart your computer before using Q Capture Pro 7 0 5 1 34 Chapter 2 Getting Started This chapter introduces you to the Q Capture Pro 7 system including an overview of its interface components and features The first thing you see when you start Q Capture Pro 7 is the Welcome screen Q 1 0 i retenh A Wilma a copies Aa ea bi i TE ae itina AIFP a I oF SF oar alt a aeri esau ics amp ine Cape Se tem Eam p ie e T S iraqi Frin it Dai ae e The Welcome screen offers quick access to tutorials help files and images You can choose any of these options to begin working with images right away Recent documents This pane displays a list of files that were recently opened Video tutorials This pane displays a list of web based tutorials for using Q Capture Pro 7 Open Click this button to open an image or file Help Click this button to access the Q Capture Pro 7 online help Upgrade Options Click this button to see if any upgrades are available The Q Capture Pro 7 Application Window Show t
108. ngs O Capture Pro 7 offers you a quick and easy method of saving your images 1 Go to the Quick Save group on the Capture tab Analysts Publication fvioyie ie al Quick Save 2 Choose Quick Save for Analysis or Quick Save for Publication If you are saving a series of images choose Save as Movie When you select Analysis you will see the following drop down menu A Analysis qi Open Saved Images i Options Quick Save for Analysis stores your images in TIF format so that they can be retrieved for analysis at another time Open Saved Images goes to the Open dialog and lets you select a previously stored image Options takes you to the File Options page where you can change your preferences When you select Publication you will see the following drop down menu la Publication Auto Open i Open Saved Images fia Options Publication stores your image in a JPG format that can be easily incorporated into a report or spreadsheet It also burns in any annotations ROIs or measurements and resizes your image The Image Context Menu Auto Open will automatically open your saved image in the Q Capture Pro 7 workspace Open Saved Images goes to the Open dialog and lets you select a previously stored image Options takes you to the File Options page where you can change your preferences The Image Context Menu Clicking the right mouse button on a image displays the following context
109. ntensity significance Secondly a Palette image includes a palette table that is actually part of the image file The pseudo color palette associated with a pseudo colored image is not a permanent part of the image It is a palette that you assign to the image while you work with it in Image Pro Pseudo color palettes can be saved to a disk file and later loaded for subsequent use with any monochrome Gray Scale 8 Gray Scale 12 Gray Scale 16 or Floating Point image The basic steps involved in pseudo coloring the active monochrome image are to 1 Select the Pseudo button from the Color group on the Adjust ribbon H Pseudacolor On Oit Spectrum M Blue Red Type H Start Te en el Divisions 256 o Spins 1 3 Check the Pseudocolor On Off to show or hide the pseudo coloring on the image 4 Change the color assignments and refine the intensity division widths This is done using the Spectrum and Type drop down menus or the color bar 5 23 Enhancing an Image 5 24 The Spectrum drop down list lets you choose which colors will be used for coloring the images Choices include Blue to Red Red to Blue Cyan Magenta and Yellow You can also define a custom color spectrum Type lets you choose RGB HSI CW HSI CWW or a custom type Specify the range of intensities you want to pseudo color The intensity range is specified using the Start and End spin buttons You can load saved pseudo color settings usin
110. nvarcea 5 20 Filters e z lt s i Options 3 Comer Traertinr Comet Cole Orgla Back gorsi hien 2 18 The Q Capture Pro 7 Application Window Select Tab The Select tab ccontains features the Clipboard ROI and Annotation features that enable you to examine and annotate an image or part of an image Q mai 2 23 F oO xX Image Tools 3 a A Capture Adjust Select Measure View Image T e EE el Copy deo Ca gt 1or gt 4 5 _ Pe tow A al a eee Be Clipboard Regions of Interest Annotate Measure Tab The Measure tab contains features that enable you to measure and analyze features in all or part of your image a Ly sa Pe g o T Image Tools G es ha Bii ae a Capture Adjust Select Measure View Image a ET Se fz f EE a he ql Se ur ae Data E gt hi Table Shape P E Measurements 4 Class View Tab The View tab contains specialized tools that let you turn different views of the image or user interface on or off i Pii FS OO Image Took Capture Adjust Select Measure View Image screen Close All Close All Reset Tools Views Took Layout id Workspace 2 19 The Q Capture Pro 7 Application Window Image Tab The Image tab is only visible when there is an image open in the workspace You can adjust the image display show it in a gallery or zoom pan on a particular portion of the image QE soln a ee Adj
111. o set the maximum number of undos allowed decide on the transparency opacity of inactive images and set preferences for closing modified images Image display options govern the display of the background areas outside of the active image They also set the Zoom attributes The Q Capture Pro 7 Application Window The Application Options control how the application behaves The General Options controls the Automatic Update Check that allows you to check for updates automatically This group also allows you to turn the Welcome screen on or off Multiple Document Interface MDI lets you control the layout of more than one document in the workspace Display options let you set the application background color control the Brightness Contrast and Gamma and determine which characteristics and the data table are displayed in the application s user interface Panels Layout lets you move the application s panels to new positions and then restore them to their original positions Q Capture Pro 7 Options pa gg Application General A Welcome Screen A Automatic Update Check Histogram amp Profile Status Bar Capture MDI Default Display MDI Layout Adoptive Multiple Document Interface Display Color Scheme Silver Brightness Contrast Gamma View Auto Characteristics Vaw Dye Lens Spati A Auto Show Measurements Data Table Space Navigator 100 Panels Layout Restore Layout Restore
112. ose the image click the Close menu item from the context menu or click the X button To remove an image from the image strip select that image and click the lt Ctrl gt or lt Shift gt keys More than one image can be removed at the same time using this method Customizing the Image Strip 9 12 Appendix A File Format Specifications Appendix A File Format Specifications Supported File Formats Q Capture Pro 7 supports the following file formats A brief description of each format will list the name used by Q Capture Pro 7 in the File Open and File Save As dialogs the typical file extensions that identify the file format and any limitations such as formats that are supported for File Open only AVI AVI Q Capture Pro 7 supports many of the video varieties of the Microsoft AVI file format which will usually have the AVI file extension The varieties that will be available in your copy of Q Capture Pro 7 depend on the AVI file compression support that has been installed by the operating system and other multi media components You cannot annotate AVI files in Q Capture Pro 7 BMP BMP DIB RLE Q Capture Pro 7 supports all known varieties of the Microsoft BMP file format which will usually have the BMP file extension but may also have a DIB or RLE extension You cannot annotate BMP files in Q Capture Pro 7 FITS FIT FTS FITS Q Capture Pro supports most types of the FIT
113. ose to ignore the message but you will not receive the update lt Close Details 2 Click Close Details to proceed 1 27 Installation 5 Click Next to continue Q Capture Pro will begin downloading the new software Q Capture Pro 7 Update Downloading File Please wait while the file is downloaded Downloading Capture Pro 7 0 4 exe Estimated time left 00 44 Transfer rate 297 89 KB Sec Q Capture Pro 7 0 4 update i gt This package will install the Q Capture Pro 7 0 4 update Do you want to ce continue 6 Click Yes to continue After downloading the file Q Capture Pro will extract the updated file Q Capture Pro 7 0 4 The next dialog shows that the update is being installed 1 28 Installation Q Capture Pro 7 Please press OK to complete installation of the Q Capture Pro 7 0 4 patch Fa reboot was indicated as necessary by the patch please remember to do so before using Q Capture Pro 7 0 4 Click OK to begin using the updated product You may need to restart your computer 1 29 Installation Updating to Version 7 0 5 New or Improved Features in Q Capture Pro version 7 0 5 1 30 This release includes pre installed capture settings created for your specific Q Imaging camera Significant changes have been made to the Calibration settings The Capture tab features a new Setup group In addition to simplifing the calibration process
114. ou can draw an array of graphical and textual annotations This section provides instructions for drawing various shapes and for placing text overlays in images The Annotate group looks like this siere Al Lal Daa k TP p ax m ss j pz bad sii Annotate Creating an Annotation Creating a Graphical Annotation There are a number of options for annotating your images with graphical overlays You can define points lines rectangles rounded rectangles ellipses polygons and polylines Line eeeceece Rectangle Rounded Rectangle Polyline Ellipse ececcee Polygon To define a graphical annotation 1 Goto the Annotate group in the Select tab s ribbon You will see these tools Ma a d G er x o T 2 i i amp 3 Burn in Annotate 2 In the toolbar click on the annotation tool you want to use and draw the annotation in the image as described for that tool below R Selection Use this tool to select an annotation object or group of objects TA Text Box Use this tool to add a text box annotation 6 3 Creating an Annotation 6 4 pe a b i i 9 9 Vv amp Rectangle or Rounded Rectangle Click the left mouse button and hold it down to drag the rectangle to your desired size Circle or Ellipse Position the crosshair cursor anywhere in the image Click and drag the mouse from the insertion point to the
115. placement program media which is free of defects in materials or workmanship you may terminate this Agreement by returning the PRODUCT and a copy of your receipt to the place of purchase and your money will be refunded Where Media is not the Manufacturer Media shall have no liability to replace or refund and you agree to look to Manufacturer to meet the obligations described above LIMITATION OF LIABILITY IN NO EVENT WILL MEDIA OR MANUFACTURER BE LIABLE TO YOU FOR ANY DAMAGES INCLUDING BUT NOT LIMITED TO ANY LOST PROFITS LOST SAVINGS OR OTHER INDIRECT oPECIAL EXEMPLARY INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THis PRODUCT EVEN IF MEDIA OR MANUPACTURER HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES FURTHER IN NO EVENT WILL MEDIA OR MANUFACTURER BE LIABLE FOR ANY CLAIM BY ANY OTHER PARTY ARISING OUT OF YOUR USE OF THE PRODUCT SOME JURISDICTIONS DO NOT ALLOW THE LIMITATION OR EXCLUSION OF LIABILITY FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES SO THE ABOVE LIMITATION OR EXCLUSION MAN NOT APPLY TO YOU TRADEMARKS Q Capture Pro 7 is a trademark of Quantitative Imaging Corp QImaging is a registered trademark of Quantitative Imaging Corp and Media Cybernetics is a trademark of Media Cybernetics Inc No right license or interest in such trademarks is granted hereunder vi U S GOVERNMENT RESTRICTED RIGHTS IN DATA This computer software product and docum
116. portion of the PRODUCT to develop other software without a license from Media or otherwise modify or create a derivative work from the PRODUCT without a license from Media 5 Re distribute any of the Microsoft R VisualBasic R components including but not limited to VBA APC Microsoft Forms Core Technology OLE Automation VBA Installation Program or the End User documentation for such components 6 Reverse engineer decompile or disassemble the PRODUCT To use the PRODUCT as described in Sections 2 or 4 above or for any other use not specifically set forth above additional licensing from Media is required For further information please contact Media at Media Cybernetics Inc 401 N Washington St Ste 350 Rockville Maryland 20850 Dem wees Phone S01 4295 3305 Fax 301 495 5964 WorldwideWeb http www mediacy com E Mail sales mediacy com PROPRIETARY RIGHTS This Agreement gives you ownership only of the physical program media on which the PRODUCT is stored but not of the PRODUCT itself You acknowledge that Media owns all right title and interest in the PRODUCT and that you will acquire no rights in the PRODUCT through your use of it You agree that you will take no action that interferes with Media s rights in the PRODUCT TERM This Agreement is effective until terminated You may terminate it at any time by destroying the PRODUCT together with all copies and documentation in any form This Agreemen
117. pt the license agreement and click Next The Select Target Application screen appears Select target application s Setup has detected multiple suitable target applications in which to install this product Please select the one s to be targeted for installation E tmage Pro Plus 7 0 o Image Pro Insight 8 0 image Pro Premier 9 1 QCapture Pro 7 0 OEM IG Application Note If there is only one available application you may not see this screen Installation Check the box to indicate which software application you are using Click Next to continue Notice the icons appearing next to the names of the various products They illustrate three possible situations Already Installed If the current version of the interface is already installed you will see the information icon and a message saying that the software is alread y installed Older Version Installed If the application detects an older version of the interface you will see the question mark icon and a message asking you if ou want to continue with the installation Downgrade Not Permitted If the application detects a newer version already exists on your system you will see a message indicating that you cannot proceed In any of these cases click the Back button to return to the Select Target Application dialog to ensure that you are choosing the correct product installation Then click Next to continue The Select Destination Folder screen appears
118. r your license to be able to receive future product updates All un registered licenses will not be able to download new updates until they are registered e If you are an existing Q Capture Pro 7 user you will receive an automatic message letting you know that a new update is available You will be asked to register your license to receive the new calibration user interface update and other future updates You may choose to ignore the message but you will not receive the update If you have NOT already registered you must register your software before you can install the 7 0 5 update for Q Capture Pro 7 If your temporary license has expired you will be sent directly to the Q Capture Pro 7 registration page 1 20 1 2 Installation If you have a hardware key dongle please plug it into your computer now If you don t have a key you may see one of these messages 1 This message appears if you are running an unlicensed copy of Q Capture Pro 7 Q Capture Pro 7 x The G Capture Pro 7 tral license i expired and the application requires to be activated Please achyate Capture Pro by entering your licensing information in the following Form 2 If you are running an unregistered copy of the software the message is slightly different Q Capture Pro 7 This is an unregistered license of O Capture Pro Although this application will run you will not receive auto updates or support until this license is regist
119. r editing it by holding down the lt Control gt key as you move the annotation Resizing Reshaping an Annotation Annotations can be resized and reshaped by moving their control points To resize or reshape an annotation 1 Click the Selection tool i from the Annotate group 2 Click on the annotation you want to resize or reshape The annotation is selected 3 Position the cursor over the control point that you want to move If the cursor is positioned correctly the Resize tool p or appears over the control point 4 Click and drag the control point to the desired location Grouping Annotations The Annotation feature allows you to group annotations in the image Annotations that are grouped are fixed in positioning relative to each other and they are otherwise treated as one object To group annotations 1 Click the Selection tool W in the Annotate group 2 While holding the lt Ctrl gt key down on the keyboard click on the annotations you want to include in the group For example annotations 2 and 3 have been selected below Arranging Annotations 3 Click on the Group button Jon the Annotate panel The selected annotations are grouped 4 To ungroup an collection of annotations click the Ungroup button Ordering Annotations The Annotation feature allows you to control the order in which annotations appear To change the order in which an annotation appears 1 Click
120. r the image or there must be a feature pictured in the image that has a known length value in terms of the units of interest see Pixels per Unit under About Spatial Calibrations above Quick Calibration Method 1 Open the image you want to calibrate or that you want to use to create the calibration definition i 2 Click the Quick Calibration Ci button in the Spatial Calibration group on the Capture tab s ribbon DD Calibration 3 Draw a line over a feature in the image You will see the Calibrate by Feature dialog Calibration parameters Calibration name a m Te unite faa Millimeter Feature size units 1 000 a Fi Show calibration dialog fk cance 4 Select a calibration name in the drop down list 3 14 The Capture Tab 5 Select the units in the drop down list and indicate the number of units in the selected feature 6 Click OK 3 15 The Capture Tab Calibrating by Aspect Ratio If your calibration must also correct for aspect ratio you must either know the value l h length divided by height for a perfect square as represented in the image or there must be a feature pictured in the image that is known to be a perfect square see Aspect Ratio under About Spatial Calibrations above To create a spatial calibration 1 3 16 Open the image you want to calibrate or that you want to use to create the calibration definition On the Capture tab s ri
121. ransform group from the Adjust ribbon amp ba Res ize Rotate Canvas Transform 3 From the Resize pull down menu select an image size EE pa 479 pM 4 Resize 5 13 Enhancing an Image 5 14 4 Click the lock icon and select a measurement unit from the drop down menu Percentage This will maintain the current aspect ratio of the image To unlock the aspect ratio click the icon again You can find more information about aspect ratios in Chapter 3 Capturing Images From the second pull down menu select the type of smoothing you require in your resized image Hone Bilinear Bicubic The resized image will appear in the workspace with the name castiron_100resz Enhancing an Image Rotating an Image Rotating an image allows you many different views of the same image For example you can rotate it 90 degrees counterclockwise or flip it left to right To try some of the image rotation features follow these steps 1 Open the image in the Q Capture Pro 7 workspace if it is not open already 2 Select Rotate from the Transform group amp ba Res ize Rotate Canvas Transformi 3 Use the pull down menu to see the Rotate tools Rotate 90 counter clockwise Rotate 180 Rotate 90 View grid clockwise Flip left right Rotate and no clip mirror image Flip up down Rotate and clip Swap lower left upper right 5 15 Enhancing an Image 4 Choose the
122. river version 2 0 9 8 or later is required to ensure correct operation Installation You will see the Q maging Capture Interface End User License Agreement End User License Agreement Please read the following license agreement carefully IMPORTANT PLEASE READ CAREFULLY LICENSE AGREEMENT THIS LICENSE AGREEMENT AGREEMENT IS BETWEEN YOU THE END USER AND MEDIA CYBERNETICS INC MEDIA IT GOVERNS THE USE OF THE SOFTWARE PROGRAM MEDIA AND DOCUMENTATION KNOWN AS O maging Capture Interface THE PRODUCT IF YOU USE THE PRODUCT THEN YOU AGREE TO THE TERMS OF THIS AGREEMENT IF YOU ARE NOT WILLING TO BE BOUND BY THE TERMS OF THIS AGREEMENT PROMPTLY RETURN THIS PACKAGE TO THE PLACE OF PURCHASE WITH A COPY OF THE RECEIPT AND YOLIR LICENSE FFF Will RE RFELINDFN V accept the terms of this License Agreement Back J Net canca Check the box to accept the license agreement and click Next The Select Target Application screen appears Select target application s Setup has detected multiple suitable target applications in which to install this product Please select the one s to be targeted for installation Z Image Pro Plus 7 0 V Image Pro Insight 8 0 V Image Pro Premier 9 1 V Q Capture Pro 7 0 Check the box to indicate which software application you are using Click Next to continue Note If there is only one available application you may not see this screen Installation 4
123. s a small unobtrusive feature that does not collect any personal information from you Most of the time product updates happen automatically If you have an Internet connection your application software will check for updates when you start the product The Options button on the Application Tools dialog allows you to customize the user interface to reflect your personal preferences Clicking the Options button displays a select of features that can be customized To turn a feature on or off check the box next to the name of the feature or select an item from the drop down menu Q Sy ke G Box Image Too Recent Documerts New 1 NUCSTAIN TIF Options button Options kg Exit Q Capture Pro 7 The Application Options control how the application behaves The General Options control the Automatic Update Check that allow you to check for updates automatically TrueUpdate is a registered trademark of Indigo Rose 1 23 Installation Select the Automatic Update Check in the Application page of the Application Options as shown here ee a ay Caos Check this box for ite rac Upee rect Automatic Update Check Lte A Tai a Muli pte Document intewtace ei Gilo faa Duna a Dena Aes A ractentoo ew Dye s Leone gt o use Shew Mese uerept eta Tabte Spex Nangoro Sero e Harris L ptat ske Leyud A DK Tmn Rett Checking this box ensures that software updates will happen automatically However you mu
124. s above the high level display as white Q Capture Pro 7 scales all intensities within the active image to take advantage of the range of black and white values available on your monitor Display range enhancements are implemented through a Lookup Table rather than by modifying the image bitmap The original appearance of your image can be restored at any time If you want to make the enhancements permanent you must write them to the image with the Apply Display to Image button To change the display range for an image 1 Select the Display group in the Adjust tab s ribbon _e eL 94 E EAIC R B 4I A Se w ma nE ps x Nea i mi v G E Advanced T amp S ABest F A E ___Displa 2 Click the Pest HE button to find the best display range automatically You can use the BL and WL spin buttons to make further adjustments to the display range Adjusting Brightness Contrast and Gamma The brightness contrast and gamma controls provide three ways to adjust pixel intensities in order to enhance objects of interest in the image They allow you to adjust the display of image intensities between the black and white levels 3 27 Enhancing an Image To adjust brightness contrast or gamma 1 5 28 Click on the Display group in the Adjust tab s ribbon O 9 o a amo ey uo 3 _ Bim Hy Q a _3 _ 1 8 Ssmi B fs Advance
125. section explain how to use a digital camera to capture images Setting up a Digital Camera Before attempting to view live images and capture them you need to select the capture driver for your camera This only has to be done once as Q Capture Pro 7 stores the settings you define so that the next time you use this camera the driver settings will be available automatically To select your digital camera driver 1 Click the Camera group on the Capture ribbon OF at 2 Click the Live drop down arrow and select one of the cameras from the drop down list ing Simulation The Capture Tab 3 Got to Settings and click the drop down arrow Select the Last Capture settings file to apply to this camera LastCapture The Last Capture settings will use the settings from your most recent acquisition Basic Steps for Capturing Images Using a Digital Camera This section provides basic instructions for using the Q Capture Pro 7 capture utility to view and capture images from a digital camera To view and capture images from your digital camera 1 Go to the Camera group on the Capture ribbon WF Arto Expos re 0 00 FP_ Live Capture Cameras 2 Click the Live drop down arrow and select one of the cameras from the drop down list 3 Click the Live button to see a live preview of your image 4 Click Capture to capture the image to the Q Capture Pro 7 workspace 3 Capture Mavie Date Time Stamp Yo
126. st register your software to download and install future updates beginning with version 7 0 5 1 24 Installation If the automatic update feature is turned off the following instructions can be used to update your Q Capture Pro 7 software though version 7 0 4 You cannot use this feature to install the 7 0 5 update even if you have a registered copy Update instructions for version 7 0 5 appear later in these pages Follow the steps below 1 From the Windows Start menu go to All Programs 2 Right click the mouse to expand the Q Capture Pro 7 menu Image Pro Insight ii Image Pro Premier 3D 64 bit y Image Pro Premier 9 1 64 bit Risa Lapidow UD Image Pro Premier 64 bit Image Pro Premier XYZ Documents Intel i iTunes Pictures ye MadCap Software Maintenance dJ Microsoft Headset P Microsoft Office Computer Microsoft Silverlight Music Control Panel W MXmeeting p1 OD Ca gt Devices and Printers Default Programs Update Q Capture Fro d Documentation Help and Support RoboSource Control 3 1 J Roxio Creator Starter d Back ood eee ea Search programs and files Shut down gt 3 Choose Update Q Capture Pro 7 If you are runing the most current version of the software you will see the following message 1 25 Installation Q Capture Pro 7 Update Check Update Check Complete Q Capture Pro 7 is up to date You already have the most recent version of Q Cap
127. stination 0 4095 Direct Copy Pixel Values Custom Conversion Pixel Values 3 Select the type of conversion to perform Your image is automatically converted to the new model Once you have set the conversion options the way that you want them you can click the Apply button in the Convert group to convert the active image using the current settings without making furrther changes 5 18 Enhancing an Image Using Color Group Commands Use the Color group commands to transform an image to another color model to extract a specific color channel from a color image or to merge an active 8 bit gray scale image into a True Color image The Color group is shown here Wao amp B Merge Dye Tint Composite Pseudo Color Use the Extract selections to extract a specific color channel from a color image and view it as an 8 bit Gray Scale image You might do this to reduce the saturation values in a HSI image or boost just the Blue values in an RGB image Use the Merge command to merge an active Gray Scale image into a True Color image This is usually done to return a channel extracted with the Extract Channel command to an image after the channel data have been manipulated However you might use it to merge any Gray Scale image to a color channel in a True Color image Dye Tint Images The Dye Tint feature lets you apply color to your image s Click on the drop down arrow to see the Dye Tint dialog Tint Image On Of
128. t tabs are named Capture Adjust Select Measure and View The Image tab is visible only when an image is open in the workspace Each tab opens a specific ribbon displaying groups of related tools Q gt T Ly ki qa Z o is ret He Capture Adjust Select Measure View Image Capture Tab The Capture tab dictates how images are brought into the application for viewing manipulation and analysis The Capture ribbon contains groups for camera control color adjustment calibration intensity adjustment and Quick Save options ee a Capture Adjust Select Measure View E a U Auto Exposure 0 00 FPS py gsm nibs BC Ca ths Pi Lens F none gab Paw Live Capt Setup D Image LIME Saturation sis Publication Movie ive aptur None Fa eae Sete r Analysis ublication Cameras Setup 1 Optical Characteristics Calibration Intensity Quick Save More information about capturing appears in Chapter 3 Adjust Tab The Adjust tab contains all the functions that allows you to manipulate the image for best viewing and interpretation Some functions may require making new or composite images from other images or changing the pixel values within an image Q a Hur Imge Took Cactus Fro T NUCSTARN TIF Captwe Adjust Select Menue View Image R E Z Bo eA Ea e a MB mao SE A n SOS wt ae ww F corca l Sabtanigie Resis Rotale Camas CEG Extract i Compote Y _3 _ t 45 Rest Fi e lt u a
129. t will also terminate automatically and without notice from Media if you fail to comply with any term or condition of this Agreement You agree upon such termination to destroy the PRODUCT and all copies of the PRODUCT DISCLAIMER LIMITED WARRANTY EXCEPT As PROVIDED BELOW THE PRODUCT IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND EITHER EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PRODUCT IS WITH YOu SOME JURISDICTIONS DO NOT ALLOW THE EXCLUSION OF IMPLIED WARRANTIES SO THE ABOVE EXCLUSION MAY NOT APPLY EO AOU NEITHER MEDIA NOR MANUFACTURER WARRANT THAT THE FUNCTIONS CONTAINED IN THE PRODUCT WILL MEET YOUR REQUIREMENTS OR THAT THE OPERATION OF THE PRODUCT WILL BE UNINTERRUPTED OR ERROR FREE However where Media is the Manufacturer Media warrants that the program media on which the software is furnished will be free from defects in materials and workmanship under normal use for a period of ninety 90 days from the date of delivery as evidenced by a copy of your receipt ws LIMITATION OF REMEDIES Where Media is the Manufacturer Manufacturer s entire liability and your exclusive remedy shall be Ta the replacement of the program media not meeting the Limited Warranty which is returned to Manufacturer with a copy of your receipt 2a if Manufacturer is unable to deliver re
130. the Advanced button You will see the Advanced tools ribbon 5 25 Enhancing an Image Sala log AY Close This tab contains all the features you may need to make and view changes to the Lookup Table As you make changes to the LUT you will seem them reflected in the LUT profile graph at the bottom of the workspace Note You will not get a dynamic LUT profile graph for AVI and SEQ files unless you select an active frame If you are working with a black and white image you can adjust the black and white levels by using the BL and WL spin buttons or typing new values in those fields Black Level EN BL 14 3 Ec ky Wk 185 5 a White Level We ese Ee p ga ua sal se A Levels 5 26 Enhancing an Image Changing the Display Range The Display Range is the range of image intensities between the low and high values inclusive of the black and white levels This feature allows you to improve the visualization of objects of interest in your images by m Excluding irrelevant image data E Boosting the contrast between relevant intensities For example the intensity values of an 8 bit gray scale image can range from zero to 255 and often the useful data covers only a small portion of that range With the Display Range feature you can set high and low thresholds for intensity values such that all pixel intensities below the low level display as black and all pixel intensitie
131. the Quick Access Toolbar 1 Click the drop down button next to the Quick Access Toolbar The following menu appears Customize Quick Access Toolbar Switch to MDI Unde Redo Image Properties Select More Commands Place Quick Access Toolbar below the Ribbon Minimize the Ribbon 2 Click on More Commands in the drop down menu The Customize Quick Access Toolbar dialog box appears Customize Quick Access Toolbar Choose commands from 5 Undo 2 Add Marker Bar Redo ep Apply Mi Delele all Overlays w Apply Display to Image Graphical Object Property 3 Auto calibrate Add 2 Open Images T Auto marker Remove Open one or several image fil r Quick Print BeF Best Ex ae P 9 Print the active document to t li Canvas default printer i Circle lad Close all Views ld Close ws v Place Quick Access Toolbar below the Ribbon The list box on the left displays all the available commands and icons The list box on the right displays those commands and icons currently displayed in the Quick Access Toolbar The Choose commands from drop down list displays different groups of available commands as shown here Using the Quick Access Toolbar Customize Quick Access Toolbar Choose commands from Close a T Switch to MDI lag New I Undo Open Redo A Print g Open Images amp Redo Add gt gt O Addo Open one or sever
132. the Quick Access Toolbar Added buttons will be retained from application session to application session a M Fs OK You can customize the selections that appear in the Quick Access Toolbar Click the drop down arrow to activate the menu A copy of the drop down menu appears here Customize Quick Access Toolbar Switch to MDI v Undo v Redo v Image Properties Select More Commands Place Quick Access Toolbar below the Ribbon Minimize the Ribbon Use More Commands to add items to the Quick Access Toolbar You can find information about adding commands in Chapter 9 In addition right clicking on any of the buttons in a ribbon displays a menu that allows you to add that button to the Quick Access Toolbar as shown here Customize Quick 4ccess Toolbar Place Quick Access Toolbar below the Ribbon Minimize the Ribbon The Q Capture Pro 7 Status Bar Frame 1 1 Dates 8 31 1998 10 23 09 AM XY 21 369 RGB 225 193 234 Pixel 2 282 The Q Capture Pro 7 Status Bar provides context sensitive information about the status of your activities including Current status of the selected or active object or window Frame number of the image in the workspace Date and time that the image was captured 2 16 The Q Capture Pro 7 Application Window Image type and size Pixel location of the cursor Image view icons to let you see a gallery view or frames in a sequence A zoom
133. the Selection tool h in the Annotate group 2 Click on the annotation you want to relocate in the order For example annotation 3 has been selected below a Click the Order back to front button in the Annotate group 2 The annotations are arranged in the order specified al 4 Toreverse the order of the annotations click the Move Front button Setting the Appearance of Annotations Deleting an Annotation To delete an annotation Click the Selection tool k from the Annotate group Click on the annotation that you want to delete The annotation is selected Click Delete adi from the Annotate group or press the lt Delete gt key Setting the Appearance of Annotations These instructions explain how to change the appearance of an existing annotation and how to set the default appearance attributes for the different annotation types Changing the Appearance of an Annotation To change the appearance of an annotation 6 10 Click the Selection tool Las from the Annotate group Click on the annotation that you want to change The annotation is selected oj j Click the Display Options button E in the Annotate group You will see the Display Options panel in the panel along the right side of the workspace Setting the Appearance of Annotations Display Options ax Ellipse Rectangle Z Polygon Label Border Z Visible O Width 1 6 Angle
134. the active playback range of the sequence By default the range is set to all frames The Rate control defines the playback speed in frames per second Loop combo box determines the action taken during playback when the end or beginning of the sequence frame range is passed Choose one of the following options b Stop p Reverse EA Use this button to click and drag the sequence toolbar o Click this button to move the toolbar to the area above the status bar Clicking the o button will move the toolbar back to the image This button also displays the Advanced Sequence Control toolbar Sequence Toolbar GOVE This version of the toolbar contains all the functions of the smaller version and displays the frame numbers also 5 35 Image Sequences The Subsample feature on the Adjust tab allows you to create a new sequence from a portion of the original sequence Subsampling builds a new sequence from copies of the selected frames The operation always starts from the first frame in the active frame range When the Interval is less than 1 the input sequence will be oversampled z Subsample Interval 2 00 ES Difference Option Diff Only Er Average ti Sumi Mex Maximum MiP Minimum Note that all sequence operations work with the selected images Multiple images can be selected in the image strip using lt Ctrl gt or lt Shift gt left mouxe clicks If no multiple images are selected the acti
135. ther Windows applications such as Excel or Word The Save button will save a picture of the graph in a file or save a table as an Excel or t xt file Print will send your graph or table to the printer Line Profile Tools MNS El O asi 1 Tools The Line Profile tools are similar to the annotation tools described earlier The table below describes each tool Le Select Use this tool to select a line profile feature or group of features mee Line Use this tool to draw a line on your image 4 6 Viewing a Line Profile Polygon Polyline This tool allows you draw poly vertices lines and polygons or freehand lines and Chek d shapes a back release To draw freeform lines and shapes and double chek Use this technique to create lines and shapes that have smooth edges Hold the left mouse down while you draw with the cursor to create the desired freeform shape Double click to complete the shape 72 eHet ick To draw polyvertices lines and polygons Ceha Use this technique to create lines and shapes that are Chieh J Chek 2 made up of straight line segments Click the left mouse button at each vertex including the beginning point of the polygon Double click to complete the polygon Rectangle Click the left mouse button and hold it down to drag the rectangle to your desired size Circle or Ellipse Position the crosshair cursor anywhere in the image Click and drag the mouse from the insertion point to t
136. tics This tool allows you to hide or show the statistics panel for the data table measurements A sample statistics panel is shown B here Mean value 0 00 233 99 67 11 15 35 Standard Deviati 0 00 48 03 26 39 3 13 Minimum 0 00 151 95 24 43 12 87 Maximum 0 00 298 05 105 75 21 92 Range 0 00 146 15 a2 32 9 05 Sum 0 00 1403 92 402 67 92 09 Index of Minimum 0 00 400 1 00 2 00 KH Show All Show Selected Measurements Hide Selected Measurements These tools let you choose to display or hide some of the measurement statistics 8 11 Viewing Measurement Data 8 12 Chapter 9 Customizing the User Interface This chapter provides instructions for the many ways you can customize the Q Capture Pro 7 application interface Q Capture Pro 7 allows you to set up the interface in the most efficient way for your work 9 1 Using the Quick Access Toolbar Using the Quick Access Toolbar The Quick Access Toolbar appears at the top left of the Q Capture Pro 7 interface o gt veut Csi eg METAB a Application o forai Takir a E button asin a itz Quick Access Toolbar gt x Aemme ijt OAA IEAM S jee N 2 mn LJP dd Ah ot een eee cutee A The Quick Access Toolbar allows you to display icons that when clicked execute a system supplied or user defined command In this way the Quick Access Toolbar provides a shortcut to commonly used functions Using the Quick Access Toolbar To Add a Shortcut to
137. ton and size of text can be adjusted on image There is a large list of date only time only date and time and ISO standard formats available The list shows the format as it appears with the current time and date on the system There are options to set the font text color and text size as either a static pixel value or a dynamic percentage of image height There are also controls to set the initial position of the text in one of the four corners of the image Use the Settings File controls to manage the camera settings files Here you can select a settings file folder and a new settings file can be loaded from the list Use Save Settings to save the current settings as a new file A new settings file may be loaded to the current list from the file system using the Load Settings button f Lou ae Settings HaDocuments and Settings tmcstravickRSiAp tonet Brightfield Capture Fluoresent Capture If you save your settings they will appear in the Settings drop down menu in the Cameras group This makes it easy for you to use the settings again The Capture Tab Saving Your Captured Images In addition to using the Save to File feature of the image settings Q Capture Pro 7 offers you a quick and easy method of saving your images 1 Go to the Quick Save group on the Capture tab kd Analysis Publication Ml Quick Save 2 Choose Quick Save for Analysis or Quick Save for Publi
138. ture Pro 7 installed on your system Please click Close to exit Click Close 1 26 Installation Updating to version 7 0 4 These instructions allow you to install the 7 0 4 update 4 If an update is found you will see the Update Available dialog Q Capture Pro 7 Update ome Se Update Available Press Next to Download and Apply this Update to Q Capture Pro 7 There is a newer version of Q Capture Pro 7 available version 7 0 4 Build 4285 This update adds resiliency to licensing and updates the driver installer to the current version Please press Next to install this update now or press Cancel to leave the existing version in place and no change will be made 1 Optional If you click the Details button you will see some additional information O Capture Pro 7 Update Update Details New or Improved Features in G Capture Pro 7 e New Q Capture Pro 7 users can start using the software immechately If you are a new user you will receive a pop up message asking you to register your license to be able to recerve future product updates All un registered licenses will not be able to download new updates until they are registered If you are an existing O Capture Pro 7 user you will will receive an automatic message letting you know that a new update is available You will be asked to register you license to receive the new calibration user interface update and other future updates You may cho
139. u E Color Composite COE Open Color Composite Panel The three demo images are combined into a single color image in the Q Capture Pro 7 workspace T S Uia disein Coke Canapeadle h w h Ti kz 7 au af in Laa al fam A m G E i mape rw pasand IT Deel Dioba Color Composie if 2S tempos The three original images and the combined image appear as thumbnails in the image strip on the left side of the workspace 5 21 Eix Enhancing an Image The color composite dialog on the right side of the workspace contains information about each image Each of the original images represents a single channel in the composite image Color Composite x We yg Apply ME Clear fee Color Composite View Ee Display 6 50 C 50 t 50 Ea DemoGreen TIF es E Dye Tint R 0 G 255 B 0 GF Display B 50 C 50 G 50 BL 0 WL 255 Ei Offset X 0 Y 0 Frame 1 Fi Binning IX E ag DemoBlue TIF E HD RY DemoRed TIF Detailed information about each color channels can be displayed by clicking on the sign to expand the image information You can make changes to the image information change the color display or remove one image channel entirely Click the eye button to show blink or hide the composite images as shown here Show Blink or Hide show current image blinking mode B hide current image in the view
140. u can add a scale bar or a date time stamp to the captured image by checking that item in the drop down list Checking Movie allows you to capture a sequence of images Once you have selected a camera Q Capture Pro 7 will remember to use that camera until you change the selection The Capture Tab Specifying Camera and Live Preview Settings This section provides instructions for adjusting commonly used capture settings that can be used to improve the quality of the live images being streamed from your camera To Use the Auto White Balance Function To have Q Capture Pro 7 adjust the white balance automatically 1 2 Go to the Camera group on the Capture ribbon Auto Exposure 0 00 FP E iy ae z Mone Cameras Click Settings drop down arrow Choose White Balance af if Dynamic Auto Range Dynamic Auto Exposure Reset To Default White Balance Camera Q Capture Pro 7 automatically adjusts the colors so that they appear through the capture window as they appear to the eye You can also set the Dynamic Auto Range and Dynamic Auto Exposure features by checking that selection Dynamic auto range uses best fit to set the display range on every preview frame Using Advanced Settings The Capture Tab The Advanced Settings allow you to adjust the capture speed and other image settings These settings allow you to for fine tune the way images appear through the Capture Preview window 1
141. ust Select Measure View Image Q b I Fan nage 238 bu Clone Views i Zoom Groups When you click on a tab you will see the ribbon with the tools and features associated with that tab arranged in groups For example these groups appear under the Capture tab Q Capture Adjust Select Measure View j NZ Auto Exposure 0 00 FPS y j of me 5 e at s z Za i ovo 99 Settings l Lens F none ti Poe gid live Capture Setup D a mage HPS Saturation Analysis Publication Movie Cameras I Setup 1 Optical Characteristic Calibration Intensity Quick Save The groups are related in some logical way The Cameras group provides different ways of acquiring images Each group contains a pull down list of items that you can edit The items in each pull down list depends on what features are installed and how often you use them Drop Down Menus The small triangles beside or underneath each tool in a group open the drop down menus _ Drop Down Menu Symbol 2 20 The Q Capture Pro 7 Application Window For example clicking the triangle next to the Settings button displays the following drop down menu i Ci Settings Dynamic Auto Range Dynamic Auto Exposure Reset To Default White Balance Camera Most of the drop down menus close automatically after you perform an action Those which contain items that you may want to use several times do not clos
142. ve image sequence is used for processing Interval This control allows you to specify the interval that will be used for the sub sampling In the example above the sub sampled sequence will be composed of every 2 frame of the original sequence Difference These controls calculate the frame to frame difference of selected portions of your sequence New images are created using the subsampled portion of your sequence The options define how the last or first frames will be processed Wrap Around Calculates the last frame as the difference between the last and first frames Diff only Return only the difference frames result will be one frame shorter than the original Pad First Pad the result with the first frame of no difference Pad Last Pad the result with the last frame of no difference Average Calculates the average of all frames of the selected images Sum Calculates the sum of all frames of the selected images Note that the output image will be a floating point type with the number of channels corresponding to the source image 5 36 Image Sequences Maximum Creates a maximum intensity projection of all frames Minimum Creates a minimum intensity projection of all frames Saving a Movie To save your image sequence as an AVT file follow the steps below 1 Go to the Quick Save group on the Capture tab Analysts Publication fvioyie T T Quick Save 2 Choose Save as Movie You will see the
143. view to set proper exposure time and avoid saturated areas so that intensity measurements on the image will be correct All of these features are discussed in greater detail in the next chapter 3 27 The Capture Tab 3 28 Chapter 4 Viewing Intensity Information This chapter explains how to E View an Image Histogram E View a Line Profile m Use the Saturation warning The Image Histogram Line Profile and Saturation Warning tools appear in the Intensity group on the Capture ribbon 4 1 Viewing a Image Histogram Viewing a Image Histogram Image histograms measure and illustrate in graph form the brightness and contrast characteristics of an image Histogram data can be created and viewed for data gathering and analytical purposes or can be manipulated for image enhancement purposes To create an image histogram 1 Open the image that you want to measure if it is not already opened 2 Select Image Histogram from the Intensity group on the Capture tab s ribbon re Line as Be oe A histogram window appears in the Q Capture Pro 7 workspace b ad S Best Fit Auto Range js ho i beeeeeeeeeeseeeeeeee dy Ln TO 0 32 i 96 128 460 102 2M The degree of detail such as the display of the x axis y axis legend and statistics are controlled by the Histogram and Profile Options found in the Q Capture Pro 7 Application Options menu described in Chapter 2 The Reset button

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