xxPRESS CENTRIFUGE user manual
Contents
1. The unit must be installed and operated on a solid stable and level working surface ensuring that the ventilation holes at the rear and underneath the unit are not obstructed WARNING XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 12 General Operating Precautions Ensure that the power is switched off at both the AC mains supply outlet and at the back of the unit before inserting or removing the mains power cord The rotor can spin very fast during operation and would cause serious injury if it came into contact with hands fingers or other soft tissue Operators must not defeat the safety locks on the unit and gain access to the rotor whilst it is still spinning The unit is intended for use with plates containing biological samples only Never use the unit to centrifuge any explosive volatile or highly reactive substances or chemicals There is a possible finger crush hazard due to the lid falling or being pushed closed CAUTION Care should be taken when opening the lid XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 13 Unit Maintenance and Serviceability There are no user or operator serviceable parts inside the unit Do not remove the unit casework Removal of the unit s casework will void the manufacturer s warranty and may expose the user to a Risk of Electric Shock resulting in serious injury or death Once installed the fused supply to the equipment will only blow under a fault condition This fuse s2. avoided result in minor or moderate injury or degrade or impair the functionality of the unit CAUTION Indicates a Risk of Crush hazard due to moving parts which could if not avoided result in minor or moderate Injury CAUTION Jury i Advisory note or other useful information gt NN Refer to Section NN for more details XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 6 3 Unit Description The xxpress Centrifuge is designed to separate solutions into fractions lt is provided alongside the xxpress Real Time PCR Thermal Cycler to ensure that the sample is correctly located at the bottom of the plate prior to thermal cycling The centrifuge can also be used in analytical chemistry microbiology virology clinical biochemistry The xxpress Centrifuge comes equipped with a rotor that will take an xxplate xxplates are the consumable test plate used by the xxpress Real Time Thermal Cycler it can also be equipped with different rotors The centrifuge has a microprocessor control system that provides smoothness of rotor rotation and controls the start duration and speed of rotation The unit has a number of safety controls including the locking of the lid during operation and a built in imbalance sensor The unit has the following external features Lid Rotor with Carrier Panel Main Emergency Opening Lever Underneath XXPRESS CENTRIFUGE USER MANUAL 1 0 The xxpress Centrifuge unit is designed
3. to be used with xxplates but there is also a range of rotors that allow the use of other sample plates and consumables some of which are listed below Table 2 Rotors and Adaptor Types Adaptor Block Type Suitable Plate Types BJS xxplate ROTOR Rotor for 2 plates Suitable for 24 54 and 96 well xxplates Top speed 2500 rom XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 8 ROTOR 6M 04 Rotor for 2 plates size of the applied plates W x Lx H 86x 128 x 20 mm Top speed 2500 rpm ROTOR 6M 05 Universal rotor for 12 adapters Maximal volume of applied test tubes 15 ml Maximal sizes of the applied test tubes D x L 16 8 x 140mm Top speed 3500 rpm ROTOR 6M 01 Universal rotor for 4 adapters Maximal volume of applied test tubes 50 ml Maximal sizes of the applied test tubes D x L 30 x 135 mm Top speed 3500 rpm Other specialist adaptor plates may be available at request Please contact your distributor for details XXPRESS CENTRIFUGE USER MANUAL 1 0 4 Technical Specification Model Type Model Name Model No Physical Unit Properties Dimensions W x D x H Weight without adaptor Mains Supply Power Cord Rating Inlet Module Type Supply Voltage Supply Frequency Range Power Consumption Fuse Ratings Operating Environment Temperature Range Relative Humidity Range Maximum Operating Altitude Storage and Transportation Temperature Range Relative Humidity Range Operating Pa
4. Transportation environmental conditions Always ensure that the unit and accessories are completely WARNING dry and free of any condensation before being packed XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 24 Certificate of Decontamination We respect the health and safety of our clients and employees and request that any products or accessories being returned are decontaminated in accordance with the procedure below 1 Decontamination Procedure Thoroughly clean all outside surfaces of the product including any accessories power cords manuals packaging etc with a damp cloth soaked with suitable disinfectant solution Such as Virkon Allow to dry fully before packing 2 Decontamination Declaration Company Name Address Product Code xxpress Centrifuge CM 6MT Serial Number Reason For Return Where Product Used Please tick the appropriate option s below O certify that have decontaminated the product as per the above procedure Decontaminant Used O certify that the product has not been exposed to any chemical or biological materials Title Name Signature Date Telephone Email XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 25 9 Warranty and Returns BJS Biotechnologies Ltd warrants the xxpress Centrifuge product when purchased new and installed and operated in accordance with the instructions of this manual to be free from defects in materials and workmanship and will repair or
5. ations for the disposal of potentially hazardous waste and electronic equipment The unit and its accessories should be decontaminated using the procedure detailed in Section 8 2 before disposal or shipping Do not dispose of this product into unsorted municipal waste or public landfill Please contact your distributor or BJS Biotechnologies at the address on Page 2 of this manual for details of how to correctly dispose of this product XXPRESS CENTRIFUGE USER MANUAL 1 0
6. both during normal use and during transportation and storage CAUTION The unit should only be held by the painted casework when being lifted or moved Do not lift the unit by the lid or rotor WARNING Care should be taken when lifting the unit due to its weight Care should be taken not to knock the LED display Do not use excessive force when pressing the control buttons CAUTION OF closing the lid XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 11 Unit Installation and Operating Environment The xxpress Centrifuge unit is designed for indoor laboratory use only The acceptable operating temperature range is 4 C to 40 C with a relative humidity of 20 to 80 non condensing at a maximum altitude of 2000m above sea level lf the unit is stored in conditions outside of these ranges it must be left to stand unpowered until it has acclimatised to within these environmental limits before being powered Use only the AC mains power cord provided with the unit or as specified in Section 4 The unit must be connected to a suitably earthed mains supply with appropriate earth leakage and over current protection Always ensure that the mains power connector is securely inserted into the rear of the unit and any excess power cord does not pose a potential trip or pull hazard WARNING Do not operate the unit in any area which is or has been or is thought to have been exposed to explosive or flammable gases vapours or liquids
7. ct the unit casework lid and moving parts for any signs of wear damage cracks or other defects Clean the casework using a damp cloth soaked with a disinfectant solution Such as Virkon whilst wearing suitable PPE Carefully clean the rotor lid and stainless steel inner casing to remove any debris or spillage Check that the xxplates carriers swing freely on the rotor Clean the display and buttons taking care to avoid over wetting Check that the ventilation holes on the rear and underneath of the unit are clear of dust and fluff build up XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 23 around the mains power inlet before reconnecting the power cord and switching the unit on SN After cleaning ensure that the unit is thoroughly dry especially DANGER 8 2 Decontamination Procedure The unit and accessories should be decontaminated using the following procedure before being stored or transported Decontamination Procedure Thoroughly clean all outside surfaces of the product including any accessories power cords manuals packaging etc with a damp cloth soaked with suitable disinfectant solution such as Virkon 8 3 Transportation and Storage The xxpress Centrifuge unit and its accessories should be thoroughly decontaminated using the procedure detailed in Section 8 2 before being placed in its original packaging for transportation or storage Refer to Section 4 for the acceptable range of Storage and
8. d AC mains power connection see Section 4 Please also observe and abide by the Unit Installation and Operating Environment safety precautions and preconditions listed in Section 5 WARNING It is important the check that the Mains Voltage is correct before use the voltage specification label is at the rear of the unit on the underside XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 15 Install and test the xxpress Centrifuge using the following procedure 1 Place the unit on the suitably selected working surface as specified above ensuring that the ventilation holes on the rear and underneath of the unit are not obstructed 2 Connect the unit to the AC mains power outlet using the mains power cord supplied or as specified in Section 4 3 Switch the mains power on at supply outlet first and then switch the unit on using the power switch located at the rear of the unit 4 Check that the unit is stable and safe by performing a trial centrifuging operation as described in Section 7 2 lf the unit has been stored in a cool environment it must be left to stand unplugged until it has acclimatised to the new room temperature before being powered DANGER XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 16 7 Unit Operation Please ensure that you have read and fully understood all of the Safety Precautions and Limitations of Use listed in Section 5 before attempting to operate the xxpress WARNING Centrif
9. eeeeeeeeeeeeeeeeeeees 5 3 Unit Description 0 0 0 ccc cecccceececeeeceeeeceeeeceeeeeeeeeeeeseueesaeeesaeesaueesseesaneeseneenes 6 4 Technical Specification ccceecceceeeceeceeeeeceeeeeceeeeseeeesseeeeeseaeeessaeeeesaaes 9 5 Safety Precautions and Limitations of US ccccceccceececeeeeeeeeeeeeees 10 6 TANG NNSA ATION erospen EEE E E E EE EEE 14 7 UA ODS aO ern E EE 16 Fal USerDisplay anda COMO Soa scseceecsccsencescescweed oesi Ni R 16 7 2 Operating the Controls of the xxpress Centrifuge ccccccseeeeeeeeeees 18 Lo NUNN VAIO I ee e E EE E ER 21 8 Maintenance and Servicing ccccccsescecceceeceeeeecseeeeceeeeesaeeeesseeesseeess 22 8 1 Routine Cleaning and Inspection ccccceccceceseeeeeeeeeseeeeseeeeeseeeeeeens 22 8 2 Decontamination Procedure ccccccccsscccssecccseeeceseeceucecsueeeseeeessueessaes 23 8 3 Transportation and StOrage ccccccccccsssceeseeeeeceeeeeseeeeeseeeeeseeeesseeeeeeas 23 9 Waran aN ETUS aaea Ees N ONS N 25 10 Podu D 00 oy E en A E nee nee eee 26 Tables Table 1 Advisory Symbol Meaningg ccccccseeeeeeeeeeceeeeeeeseeeeneneees 5 Table 2 Rotors and Adaptor Types cccccccseeceseeeesaeeeeeeesseeeeseeeeeas 7 Table 3 User Buttons and Status LEDGS ec ceccccceeeeeeeeeeeeeees 16 XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 4 1 Glossary of Terms and Abbreviations ANSI BJS Bio EMC Microtitre P
10. entrifuge will beep to indicate that the cycle is complete and automatically release the lid lock making it ready for sample removal To centrifuge that sample further the lid will need to be pressed down and locked The xxpress Centrifuge can be stopped in mid cycle by pressing the START STOP button once then waiting for the rotor to come to a stop and the lid lock to be automatically released To enter GELL mode which is used for size exclusion chromatography select G force settings and then using the UP DOWN buttons select the minimum setting XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 21 7 3 Fault Diagnosis If the xxpress Centrifuge does not start you hear a beep and the displays show ErOP it means that the lid is not closed properly Press on the lid and start the centrifuge lf the xxpress Centrifuge does not start you hear a beep and the displays show Erbd it means that there is a mechanical obstruction of the rotor or malfunction in the engine At the end of centrifugation cycle the rotor will stop and a sound signal will sound and lid will automatically unlock lf the overall imbalance of test tubes in the rotor exceeds 5 grams the xxpress Centrifuge when reaching 1000 rpm will start emergency braking and the displays will show Erdb error code imbalance Below are a list of all possible error codes MIN display X1000 Sound Cause Remedy display signal a Opened lid Close by pressing on the lid Rotor or
11. he xxplate onto the one of the carriers sample side up as shown in the picture below The rotor should always be loaded symmetrically to minimise imbalance so place another xxplate in the other carrier if it is empty The total imbalance should not exceed 5 grams Kr XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 19 Close the lid and press down until you hear or feel a click indicating that the lock has engaged Set the speed and duration of the cycle using the buttons under the displays Press the UP or DOWN arrows until the desired value is obtained Sky Line p Duration is in minutes and can be set between 1 and 99 Speed is in RPM and can be set between 100 and 3500 in steps of 100 XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 20 It is possible to change the speed reading to G force by pressing both the UP and DOWN arrows simultaneously for 5 seconds Once selected the G Force reading can be adjusted between 0 1 and 2 3 in steps of 0 1 it is measured in RCF MA Once the desired parameters are set press the START STOP button once to start the cycle The xxpress Centrifuge will ramp its soeed to the desired maximum and hold until the cycle is complete NOTE that the centrifuge can take 30 seconds to achieve maximum speed Once the cycle is complete the xxpress Centrifuge will cut the drive to the rotor and let it slow down This will take a few seconds Once the rotor has come to a halt the xxpress C
12. hould only be changed after the unit has been thoroughly inspected by a qualified engineer Always switch off the unit and disconnect the power cord before performing any cleaning or decontamination procedure If liquid is spilt into or over the unit switch off and disconnect the power from the AC mains outlet before attempting to deal with the spillage The use of harsh chemicals and cleaning agents may damage the unit and degrade its performance Always follow the cleaning and decontamination procedures CAUTION specified in Sections 8 1 and 8 2 of this instruction manual Do not autoclave any part of the unit or its accessories CAUTION XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 14 6 Unit Installation Before installing the xxpress Centrifuge please check that the delivery is complete and that the unit and any accessory parts are intact and free from any signs of transportation damage Also ensure that all external and internal packaging has been removed from the unit before installation Please retain all packaging for future transportation and storage of the unit and its accessories The centrifuge should be installed in a location which meets the following requirements e Safe and suitable operating environment see Section 5 e Solid stable level working surface e Atleast 10cm clearance around the unit to adjacent objects and walls e Sufficient clearance to enable the lid to be opened fully e Earthe
13. late MTP PCR PPE RCF RPM SBS Semi skirted PCR Plate Skirted PCR Plate Un skirted PCR Plate Well xxplate American National Standards Institute BJS Biotechnologies Limited UK Company Number 02959160 Electro Magnetic Compatibility Plate with an SBS footprint featuring 24 48 96 or 384 wells Microtitre plate Polymerase Chain Reaction Personal Protective Equipment Relative Centrifugal Force Revolutions per Minute society for Bio molecular Screening PCR plate with an outer surrounding half edge PCR plate with an outer surrounding edge PCR plate without an outer surrounding edge A single sample cavity in a Microtitre plate PCR plate or Deepwell plate BJS Bio PCR test plate for the xxpress real time thermal cycler Available in 24 54 and 96 well formats XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 5 2 Symbols Used in this Instruction Manual The following advisory symbols are used in this manual Table 1 Advisory Symbol Meanings Indicates a Risk of Electric Shock which could if not avoided result in serve injury or death Indicates a Burn Hazard which could if not avoided result in serve injury or death Indicates a Risk of Explosion which could if not avoided result in serve injury or death Indicates a hazardous situation which could if not avoided result in serve injury or death or severely damage the unit WARNING Indicates a hazardous situation which could if not
14. motor is Remove any mechanical H blocked obstacle and check rotor moves freely Total imbalance Reload the rotor to get of test tubes is symmetrical loading more than 5gr XXPRESS CENTRIFUGE USER MANUAL 1 0 Not enough Use voltage stabilizer voltage to run the centrifuge Motor overheat Check that there are no obstacles blocking the air circulation vents of centrifuge Residual rotation Wait till the rotor stops of the rotor Page 22 8 Maintenance and Servicing Although the xxpress Centrifuge unit does not require any scheduled maintenance or servicing the operator should regularly clean and inspect the unit for any detects as described in Section 8 1 below Please observe and comply with all of the Unit Maintenance and Serviceability precautions listed in Section 8 Never removed the unit casework There are no user or operator serviceable parts inside the unit Always switch off and unplug the unit before performing any cleaning or disinfecting tasks For technical and service related enquiries please contact your distributor or BJS Bio at the address given on Page 2 of this manual 8 1 Routine Cleaning and Inspection The unit casework should be cleaned and inspected at regulator internals and whenever contamination or spillage occurs as follows 1 Switch off the unit and disconnect the power before performing any inspection checks or cleaning Before cleaning always inspe
15. rameters Rotor Speed Maximum Centrifugal Force Rotor Speed Accuracy Duration Range Max Sound Level XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 9 xxpress Centrifuge by Elmi CM 6MT 430 mm x 400 mm x 225 mm 10 kg IEC C13 3 Core 5A min IEC C14 DPST 220 to 240 VAC 10 50 to 60 Hz 5 320 W max Where cable plug fused 3A fuse should be used 4 to 40 C 20 to 80 non condensing 2000 m above sea level 10 to 50 C 20 to 95 non condensing from 100 to 3 500 RPM 2 300 RCF 2 1 to 99 minutes 55db A at 1 metre Page 10 5 Safety Precautions and Limitations of Use It is essential that all users of this equipment have fully read and understood the following safety precautions and limitations of use before installing or operating the xxpress Centrifuge unit IMPORTANT The protection provided by this equipment may be impaired if it is not used in a manner described in this manual WARNING It is essential that the user of this equipment is aware of the potential hazards associated with the unit and its accessories All operators should be familiar with the safety precautions and warnings given in these instructions before attempting to operate the unit WARNING Improper use of this unit or its accessories may impair their functionality and invalidate the manufacturer s warranty Unit Handling Precautions Care should be taken not to drop the unit or subject it to rough physical handling
16. replace at their discretion any unit or accessory which exhibits such defects In no event will BUS Biotechnologies be liable for any indirect incidental or consequential damages resulting from any defect or warranty claim Unspecified use or unauthorised modification of any part of the xxpress Centrifuge unit or its accessories or the use or attachment of any adaptor or peripheral not supplied specified or sanctioned by BJS Biotechnologies will invalidate this warranty This warranty is provided to the original purchaser of the product for one year from the date of purchase Under the terms of this warranty the product must be returned in its Original packaging transportation prepaid by the sender with a copy of the Proof of Purchase and a detailed description of the problem The product must be decontaminated using the procedure detailed in Section 2 and a Certificate of Decontamination Supplied with any return If the product is considered too hazardous to be shipped please contact BUS Biotechnologies WARNING on the number given on Page 2 of this manual for further instructions Please contact your distributor or BUS Biotechnologies on the number given on Page 2 of this manual to assess the defect and arrange the return the product if necessary XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 26 10 Product Disposal At end of life this product must be disposed of in accordance with your local authority regul
17. uge 7 1 User Display and Controls The xxpress Centrifuge consists of a stainless steel inner casing within a painted steel housing a rotor is mounted in it powered by an electric motor and control system Rotor is covered with self locking transparent lid In the back of the casing the power switch is mounted On the front of the casing a control panel is placed with displays and buttons having the followings functions The button and LED functions are listed in Table 3 Table 3 User Buttons and Status LEDs Displays the time remaining on the chosen cycle in minutes XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 17 Displays the Speed or G force of the rotor during operation Rotational Speed measured in RPM Revolutions per minute Rotor rotation measured in RCF Button to increase reduce the parameter Button to unlock the lid of the xxpress Centrifuge Button to Start or Stop the xxpress Centrifuge cycle Note To change RPM to RCF G force or vice versa press and hold the buttons under the x1000 display for 5 seconds XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 18 7 2 Operating the Controls of the xxpress Centrifuge When the unit is switched on it recalls the last operation settings and displays the speed G force and the duration depending on the display mode Press the OPEN button to unlock the lid then lift the lid from the front edge to provide access to the rotor Load t
18. xxpress Centrifuge PCR plate centrifuge with programmable Speed and duration BJS BIOTECHNOLOGIES A LIFESCIENCE COMPANY This Manual Copyright 2014 BJS Biotechnologies Page 2 xxpress xxplates xxsealer and their respective logos are registered trademarks of BJS Biotechnologies Ltd or one of its affiliated companies in the UK and other countries All rights reserved No part of this manual may be reproduced stored in a retrieval system or transmitted by any means or in any form without the prior permission of BUS Biotechnologies Lid BJS Bio The information contained in this manual is subject to change without notice BUS Bio assumes no responsibility for any errors that may appear in this or related documentation Document XP 5002 01 1 Revision 1 0 May 2014 xxpress xxplates and xxsealer are the brand names for BJS Biotechnologies own range of PCR thermal cycler products and accessories For further details please visit our web site www xxpressPCR com All sales and technical enquiries should be addressed to BJS Biotechnologies Ltd 65 Bideford Avenue Perivale Greenford Middlesex UB6 7PP United Kingdom 44 0 203 021 3750 Ssales xxpressPCR com or support xxpressPCR com XXPRESS CENTRIFUGE USER MANUAL 1 0 Page 3 Contents 1 Glossary of Terms and Abbreviations ccccccccseeeeseeeeceeeeseeeeeaeeeeaees 4 2 symbols Used in this Instruction Manual ccceecseee
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