J.R. Simplot Innate™ Screening Test
Contents
1. Result If the color of the potato test vial falls in the J R Simplot Innate color range up to two more 10 minute incubation times should be allowed for the test reaction to complete If the color of the potato test vial still falls in the J R Simplot Innate color range after the additional two 10 minute incubation periods then the potato is potentially a J R Simplot Innate and must be referred to a laboratory for confirmation see Page 9 for details Follow up Genetic Testing Follow up Genetic Testing In the event that the test results suggest a potential J R Simplot Innate potato follow up genetic testing by a third party laboratory is required for confirmation Please contact the following testing facility for all indicated potato samples Follow up Genetic Testing Facility Eurofins BioDiagnostics 507 Highland Drive River Falls WI 54022 715 426 0246 Hazardous Components Safety Guide The J R Simplot Innate Screening Test kit has components which contain chemicals The following table details the safety information for all chemical contents Wear gloves and appropriate PPE Follow all safety instructions and good laboratory practices Component Hazardous GHS Symbol Hazard Precautionary Contents Statement s Statement s Indicator n a Solution D Indicator Pyrocatechol H301 H311 P201 Tablets H315 P202 H317 P261 DANGER H318 P264 H332 P270 H341 P271 H401 P272 P273 P280 P301 P2. or Wildtype for the potato being tested is indicated by comparing the color of the potato test vial to the Results Color Strip Potential JR Simplot Innate l Repeat Test l Wild type Results interpretation Depending on the type of potato being tested and ambient conditions the indicator reaction may take longer than 10 minutes to complete Wildtype Result A test vial containing wildtype potato pulp should turn brown in color and fall within the wildtype portion of the test Results Color Strip after the 10 minute incubation time Repeat Test Result If after the initial 10 minute incubation time the test vial falls within the Repeat Test color range then up to two more 10 minute incubation times should be allowed for the test reaction to complete Compare the potato test vial color to the Results Color Strip after each 10 minute incubation time If after each of the additional incubation periods the color of the test vial still falls in the Repeat Test range then the entire testing process must be repeated from the start Be sure to peel a fresh section of potato serrate and pulp the potato surface sufficiently and add indicated volume of pulp to the test vial After repeating the entire testing procedure if the potato being tested still falls in the Repeat Test color range then the J R Simplot Innate status of this potato is unable to be indicated by this kit J R Simplot Innate
3. 310 P302 P352 P304 P340 P305 P351 P338 P310 P322 P330 P333 P313 P361 P405 P501 Hazard Statement s H301 H311 Toxic if swallowed or in contact with skin H315 Causes skin irritation H317 May cause an allergic skin reaction H318 Causes serious eye damage H332 Harmful if inhaled H341 Suspected of causing genetic defects H401 Toxic to aquatic life 10 Hazardous Components Safety Guide Precautionary Statement s P201 P202 P261 P264 P270 P271 P272 P273 P280 P301 P310 P302 P352 P304 P340 P305 P351 P338 P310 P322 P330 P333 P313 P361 P405 P501 Obtain special instructions before use Do not handle until all safety precautions have been read and understood Avoid breathing dust fume gas mist vapours spray Wash skin thoroughly after handling Do not eat drink or smoke when using this product Use only outdoors or in a well ventilated area Contaminated work clothing should not be allowed out of the workplace Avoid release to the environment Wear protective gloves eye protection face protection IF SWALLOWED Immediately call a POISON CENTER or doctor physician IF ON SKIN Wash with plenty of soap and water IF INHALED Remove victim to fresh air and keep at rest in a position comfortable for breathing IF IN EYES Rinse cautiously with water for several minutes Remove contact lenses if present and easy to do Continue rinsing Immediately call a POISON C
4. Ca OMEGA Innovations in nucleic acid isolation bio tek Product Manual J R Simplot Innate Screening Test G4699 50 50 preps July 2015 For research use only Not intended for diagnostic testing J R Simplot Innate Screening Test Table of Contents Introductio te 2 Kit Contents Storage and Stability esse ses sesse eek see Gee een oe eke ee 3 Preparirid Reagents ses bies Gee Reese oe Se ee ske Ak iiaeiai 4 Test Setup Procedur ineen GE oe eet ER ke degie ese ee 5 Potato Preparation Procedure sesse see eek ee ee RR eek ee ee 6 Potato Test PRO Cet We ee se ek Ge ee Ee ee EI 7 Potato Screening Procedure ee Re Re Ge Ge 8 Follow up Genetic TeStING asssenss sesse seke EP ske EER EES RE ke egKR dekke ses 9 Hazardous Component Safety Guide sesse sen seek ee eene ee 10 Manual Revision July 2015 N OMEGA bio tek Innovations in nucleic acid isolation Introduction The J R Simplot s Innate Screening Test Kit is designed to identify J R Simplot s Innate potatoes with a fast and easy to use colorimetric test Potatoes are cut open and the inside surface flesh is scraped to form a pulp A small amount of the pulp is then mixed with Indicator Solution and incubated for ten minutes In the presence of the J R Simplot Innate potato the indicator solution will not significantly change in color However in the presence of wild type potato the Indicator Solution will turn an amber color ranging from light t
5. ENTER or doctor physician Specific measures see supplemental first aid instructions on this label Rinse mouth If skin irritation or rash occurs Get medical advice attention Remove Take off immediately all contaminated clothing Store locked up Dispose of contents container to an approved waste disposal plant For complete MSDS please visit www omegabiotek com call toll free to 1 800 832 8896 or email requests to info omegabiotek com 11 Notes
6. an to dark brown The whole procedure should take less than 30 minutes from start to finish This test is designed to be a screening test only Definitive potato J R Simplot Innate status needs be determined by an appropriate genetic testing laboratory If a positive result or ambiguous result is identified that same tuber would need to be sent for follow up genetic testing Potato tubers cannot be pooled or multiple tubers processed in one preparation Multiple preps can be processed simultaneously Kit Contents Storage and Stability Upon receipt of the testing kit place the entire kit at room temperature 20 28 for up to 6 months Once the Indicator Tablet has been added to Indicator Solution store at 2 8 C for up to 6 weeks Preparing Reagents Add one Indicator Tablet to a 60 mL bottle of Indicator Solution Invert the bottle several times during a ten minute incubation period to allow the tablet to dissolve Some insoluble material may remain after ten minutes but this will not affect the test results Write the date the tablet was added on the bottle label If stored at 2 8 C the solution is good for up to 6 weeks Use this Indicator Solution for all tests until depleted then create a new bottle of Indicator Solution as above Test Set Up Procedure J R Simplot Innate Screening Test Protocol All steps are to be performed at room temperature Materials and Equipment to be Supplied by User Potatoes for testi
7. ng Gloves Test Set Up Procedure 1 Using the small plastic scoop transfer a level scoopful of the dry powdered Postive J R Simplot Innate Control Material to an empty 2 5 mL vial This vial should be labeled as the Postive J R Simplot Innate Control 2 Wipe the scoop clean of positive control material 3 Using the same small plastic scoop transfer a level scoopful of dry Wildtype Control Material to an empty 2 5 mL vial This vial should be labeled as the Wildtype Control 4 Wipe the scoop clean of Wildtype Control material Note Only one Postive J R Simplot Innate Control vial and one Wildtype Control vial are needed for any single potato test or batch potato test Potato Preparation Procedure Potato Preparation Procedure 1 Use the serrated plastic knife to carefully cut off the end of the potato exposing a flat surface Using the serrated edge of the plastic knife apply slight pressure and roughly scrape the exposed potato surface using back and forth or side to side motion to form a surface layer of pulped potato Note This scraping process is important because the testing process occurs in a solution and requires a maximum amount of potato material to be exposed to the liquid Use the plastic scoop to gather and collect an approximately level scoop of pulp material Using a toothpick to assist transfer the collected potato pulp into an empty 2 5 mL vial This vial should be labeled as a
8. potato test vial Repeat Steps 1 3 for each potato being tested creating a new potato test vial each time Use anew scoop for each potato being tested Potato Test Procedure Potato Test Procedure 1 Using a transfer pipette add Indicator Solution 1 5 mL to all of the 2 5 mL control and potato test vials up to approximately the bottom of the threaded vial cap portion Cap the vial and vigorously shake for 2 3 seconds Repeat for all test vials Return the Indicator Solution to 2 8 C after addition to all the vials 2 After adding and mixing let all test vials sit at room temperature for 10 minutes Note The window for properly interpreting results lasts for 30 minutes after addition of the Indicator Solution Potato Screening Procedure Potato Screening Procedure 1 Compare the color of the control vials to the Results Color Strip The Positive J R Simplot Innate control vial should be light in color and fall within the color range labeled Potential J R Simplot Innate The negative control vial should be dark in color and fall within the color range labeled Wildtype If the control vials do not fall within the specified color ranges the test is invalid and the test setup should be repeated If the control vials fall within the specified control ranges then the reagents are functioning properly and interpretation of the test potato vial can be performed The status of Potential J R Simplot Innate
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