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Eclox Manual

1. ccccccceeeeeeeeeeeeeeeenees 117 Table of Contents EcloxTOC fm Page 5 Table of Contents Table of Contents Page 6 EcloxTOC fm Section 1 Specifications Specifications are subject to change without notice Dimensions 520 x 450 x 215 mm 20 5 x 17 5 x 8 5 in Weight 9 kg 20 Ib fully loaded Temperature Tested from 20 to 55 C 4 to 131 F Modes Chemiluminescence Toxicity Testing Luminescent Bacteria Toxicity Testing Arsenic Chemiluminescence Toxicity Chlorine Color Chemical Tests Nerve Agents Pesticides pH Total Dissolved Solids Conductivity Certification CE marked Up to 60 test results recorded in full detail Up to 100 luminescent measurements for Luminescent Data Logging Bacteria Toxicity Test Up to 100 screening results for Luminescent Bacteria Toxicity Test Battery powered alkaline cell lithium cell AA CAUTION For quality and safety reasons only alkaline batteries should be used with this instrument Use of other batteries may reduce the functioning of and or damage the instrument electronics by overloading the electronics or depending on the battery type can cause fire or an explosion Light d ion for th Two decades in two different ranges ight detection for the 20 1000 relative units default mode Luminescent Bacteria i Toxicity Test 20 2000 relative units o Precision 2 coefficient of variation Arsenic Range O to 4 mg L Limit of detection 0 01 mg L
2. cccccceeeeeeeeeeeeeeeeeeseeeeeaees 66 11 13 3 Show or send screening luminescence reSuUlts cc ceeeeeeeseeeeeeeeeees 66 11 13 4 Show or send LIMIT measure results cccecceeeeeeeeeeeeeeeeeeeeeeeeens 67 Section 12 Luminescent Bacteria Toxicity Test according to ISO 11348 part 3 00 0 ccccceceeeeeeeeeeeeeeeseeeeeeeeseeeeesseaeeeesaaeeees 69 TA WW OVCIVICW oases het ee eee et aa ees aee eee tae 70 N22 ZAG CACY aso oe tates trite ate pres Sascha tartare ented nea era dtiad ented bin Ra ated attended EA tekans 71 Table of Contents Page 4 EcloxTOC fm Table of Contents 12 3 Thermostat and PC software requirement ccccceeececeeeeeeeeeeeseeeeeeeeeeeees 72 124 RREAGENU GESCEDUOM essiri n a et edateuion eons 73 12 5 Reagent storage And PreServatiOn cccceccceseeceeseeeeeeeeeeeseeeeeseeeeeeeeeeseeeeeees 74 126 Prepare ING reagen nae vee ea cies ali a aetna te aes 15 12 6 1 Prepare the stock suspension using the LCK491 reagent 15 12 6 2 Prepare the test SUSPENSION c ccccceeeeceeeeceeeeceseeceasecsucesseeesseeeseas 76 12 7 Sample collection storage and preservation ccccseecceseeceeeeeeeeeeeeeseeeeeens 82 128 InererengES cts tees aa halal osetia ne ye etal A ed ay oth i a 83 1229 Prepare NG Sal PIO ise dar side eoalea a a 84 12 10 Prepare the dilutions SELIOS ccccccccssccceeeeceeeeceeeceeeeceeseceeeceseceeesseeseaes 85 12 10 1 Prepare a 9 di
3. 11 6 Prepare the stock suspension using the LCK491 reagent Prepare the stock suspension by adding the reconstitution solution to the freeze fried bacteria reagent The reconstitution solution rehydrates the bacteria reagent Reconstitution solution is specially made non toxic ultra pure water Do not make reconstitution solution or use substitutes The dry reagent can be kept at ambient temperatures not higher than 25 C up to 5 days without cooling Make sure that reactivation conditions are as cool as possible 51 The stock suspension can be kept in a refrigerator as long as the validity criteria are met typically up to 4 hours This procedure is temperature sensitive 1 Remove the 2 Put the frozen 3 In the field remove the luminescent bacteria test luminescent bacteria cap from the reconstitution reagent from the freezer reagent refrigerated solution bottle Remove the reconstitution reconstitution solution and solution and Diluent from Diluent in a cool box that refrigerator contains thermal packs if possible 1 F t g 1 00 4 Remove the foil sealand 5 Set the 1 0 5 0 mL 6 Put the end of the rubber stopper from the pipette to 1 0 mL 1 0 5 0 mL pipette in to a reagent bottle clean pipette tip 52 Gia 4 7 Put the tip of the pipette 8 Put the tip of the pipette 9 Put the rubber stopper in in to the reconstitution in to the luminescent the reagent bottle Swirl the solution and slowly
4. The Previous Measurements Menu is shown 6 To show all or send all of the results saved on the luminometer select one option e To show the results on the luminometer select Show all R1 to Rx and push ENTER e To send the results to the computer select Send all R1 and Rx to PC and push ENTER e To send the results to the printer select Send all R1 to Rx to Printer and push ENTER Note At this stage the results can not be sent to the LUMISsoft 4 7 To show or send a specific range of results saved on the luminometer select one option e To show the results on the luminometer select Show selection and push ENTER e To send the results to the computer select Send selection to PC and push ENTER e To send the results to the printer select Send selection to Printer and push ENTER 8 If an option in step 7 was selected select the data to be recalled a Select the starting indicator in the From field Push SELECT to change the value Then push PROCEED b Select the ending indicator in the To field Push SELECT to change the value Then push SHOW 9 Push PROCEED to show more results 109 110 Section 13 Maintenance Important Note All cleaning and maintenance of the Eclox Rapid Response Test Kit should be done in a suitable clean dry area Make sure that the kit is clean before removing any access or battery covers Do not let foreign material enter the kits as equipment damage can occur 13
5. 30 17 The Inhib is shown on the screen Record the Inhib value and graph on the Test Record Sheet 18 Push PROCEED to go back to the Measure Menu Note For sea water samples the graph may appear higher than the reference and the Inhib may be negative wr EZ 20 Sign the Test Record Sheet Put the sample from the beaker in the wastewater drain using local operating procedures 4 5 Show the previous results Up to 60 previous results samples plus references and graphs can be saved on the luminometer and then shown later on the luminometer To save previous results to a computer refer to Send previous results to a computer on page 31 To show previous results saved on the luminometer 1 Push ON green button for several seconds to apply power to the luminometer The luminometer does built in tests that make sure that the electronics and software are operating correctly When the built in tests are done push PROCEED The Main Menu is shown Select ECLOX and push ENTER Select Previous Results and push ENTER The Previous Results Menu is shown To show previous results a Select Recall Results and push ENTER b Push MORE to show more results c Push QUIT to go back to the Previous Results Menu To show previous graphs a Select Recall Graphs and push ENTER b Push UP to move through the saved samples c Push SELECT when the required graph number is shown to select a grap
6. Chlorine free Range 0 3 5 mg L Chlorine total Range 0 3 5 mg L Range O to 100 0 to 500 APHA Platinum Cobalt Color units Warranty Six months from the date of shipment Pocket Pal TDS Tester Range 10 to 1990 uS cm 2 of reading at 25 C calibration and 25 C sample Accuracy 10 of temperature compensated uS cm readings over 0 to 50 C range Warranty Six months from the date of shipment Section 2 General information In no event will the manufacturer be liable for direct indirect special incidental or consequential damages resulting from any defect or omission in this manual The manufacturer reserves the right to make changes in this manual and the products it describes at any time without notice or obligation Revised editions are found on the manufacturer s website 2 1 Safety information Please read this entire manual before unpacking setting up or operating this equipment Pay attention to all danger and caution statements Failure to do so could result in serious injury to the operator or damage to the equipment Make sure that the protection provided by this equipment is not impaired do not use or install this equipment in any manner other than that specified in this manual CAUTION Chemical Hazards Always follow appropriate laboratory safety procedures when handling chemicals Always wear all personal protective instrument appropriate to the chemicals you are handling 2 1 1 Use of hazard
7. The Main Menu is shown 3 Select ECLOX or Luminescent Bacteria Test and push ENTER Either option can be selected for this procedure 4 Select Set Up and push ENTER The Set up Menu is shown 5 Select Set Screen Contrast and push ENTER The Set Contrast screen is shown 6 Push DOWN or UP to change the contrast The screen shows the contrast level with a Max Min indicator bar 7 Push DOWN and UP at the same time to save the changes The Set up Menu is shown 3 3 2 Set the waiting time and measuring time This procedure only applies to the Luminescent Bacteria Test The measurement of the light intensity of luminescent bacteria is divided up in to two parts e Waiting time the amount of time the luminometer waits after the test tube is put in the lid is closed and MEASURE is pushed before measuring the light intensity from the test tube The luminometer needs to wait a few seconds to compensate for the high light level of the open lid e Measuring time the amount of time the sample is measured by the luminometer Note There is no need to change the default settings of 8 seconds waiting time or 7 seconds measuring time unless HACH or HACH LANGE customer service asks the user to do so 18 To show or change the waiting time and measuring time 1 Push ON green button to apply power to the instrument The instrument does built in tests that make sure that the electronics and software are operating corr
8. rack for 8 reaction each LCW490 vessels stand for 40 test tubes variable pipette 0 2 1 0 mL variable pipette 1 0 2 0 mL pipette tips and timer clock Case each 46608 00 Dilution solution 1000 mL each LCX048 2 NaCl solution 250 mL each LCK481 NaCl solid in bottle with dosing spoon 25 g each LCX058 Pipette variable 0 2 1 0 mL each BBP078 Pipette variable 1 0 5 0 mL each BBP065 Pipette tips for variable pipette BBP078 100 pkg BBP079 Pipette tips for variable pipette BBP065 75 pkg BBP068 Plastic test tubes for Eclox 500 pkg LZP1480 Rack for 8 reaction vessels each LYW918 Reaction vessels with cap 5 pkg LZPO65 Reconstitution solution 50 mL each LCX047 Stand for 40 test tubes each ETS018 119 Replacement parts continued Description Qty Item number Timer clock each LZC902 120 Section 16 Contact Information HACH Company World Headquarters P O Box 389 Loveland Colorado 80539 0389 U S A Tel 800 227 HACH 800 227 4224 U S A only Fax 970 669 2932 orders hach com www hach com Repair Service in Latin America the Caribbean Asia Indian Subcontinent Africa Europe or the Middle East Hach Company World Headquarters P O Box 389 Loveland Colorado 80539 0389 U S A Tel 001 970 669 3050 Fax 001 970 669 2932 intt hach com HACH LANGE LTD Unit 1 Chestnut Road Western Industrial Estate IRL Dublin 12 Tel 353 0 1 46 02 5 22 Fa
9. 1 General maintenance The Eclox Rapid Response Test Kit is made for field use Routine maintenance does not have to be done if all cleaning test and calibration procedures are done 13 1 1 Cleaning the kit Keep the kit in good condition and clean to get reliable results Clean the kit before it is put in to storage Complete a decontamination form and put the form with the kit 13 1 2 Cleaning the luminometer Keep the luminometer clean at all times If the surface is dirty clean the surface with a damp cloth Important Note Do not let water get in to the luminometer cell If water gets in to the cell remove the cell insert and remove the moisture with a clean dry cloth Replace the cell insert 13 2 Decontamination If the Eclox Rapid Response Test Kit comes in to contact with any chemical warfare CW agent decontaminate the kit before it is used again When the carrying case for the Eclox Rapid Response Test Kit is closed the kit is waterproof and can be sprayed wetted The case is chemically made hard The outside of the luminometer is also CW agent resistant and can be decontaminated after a CW attack None of the other kit components are CW agent resistant and in the event of exposure when the lid is open the kit is contaminated and must be quarantined for disposal 111 13 3 Battery replacement 13 3 1 Luminometer battery replacement 1 Remove any excess water from the luminometer Measurement errors will occur
10. 38 Section 7 Free and Total Chlorine Test 7 1 Measuring hints and general information e Clean all labware between tests Contamination may change test results Clean labware with a non abrasive detergent or a solvent such as isopropyl alcohol Use a soft cloth for cleaning or drying Do not use paper towels or tissue on plastic tubes as scratches may occur Remove detergent or solvent with clean water preferably demineralized water e Clean all viewing tubes thoroughly with the sample water before testing e To open PermaChem powder pillows 1 Tap the bottom of the pillow on a hard surface 2 Open the pillow along the dashed line 3 Open the pillow and pinch the side edges to make a spout 4 Put the contents in the sample e Accuracy is not affected by undissolved powder e The manufacturer strongly recommends that for optimum results the user make sure the reagent is accurate for each new lot of reagents Use the standard solution included in this kit or listed in Replacement parts and accessories on page 117 Do the instructions included with each standard solution 7 2 Free chlorine procedure 0 3 5 mg L 5 5 1 Filla viewing tube tothe 2 Put the tube in the 3 Fill another viewing first 5 mL line with sample top left opening of the color tube to the first 5 mL water This is the blank comparator line with sample water 39 gt 5 4 Add the contents of one 5 Swirl to mix 6 Put the
11. 47 10 2 CaliDi avons i232 sae cae eS lo ea epee ae Le 48 Section 11 Luminescent Bacteria Toxicity Test Screening and LIMIT Me SUPFE ccccccecccecceeeeeeeeeeeeeesaeeeeeeesaeeeesaeeeeesaeeees 49 II OVENI ies Ste eh eo a eases Sansa eee aoe ant Ses 49 TAZ AC CUNAGCY arenie E linge 50 didico FREAGEAE GESCHIPU OM iaiiuter ated oat ca N E E 50 11 321 Qu ality assurance test erisip a a eee 50 11 4 Reagent storage and preservatliONn ccccccssseccseeeceseseeeeeeeeessesetenseeeeneneeens 51 Wicd Prepare Ane TEAGS Mia esicicescimccewenchowescatnndetdaesaeteantea antoncvaetandeenn R 51 11 6 Prepare the stock suspension using the LCK491 reagent cccceeeeeeeeees 51 11 7 Prepare the test suspension cccccccceseeceeeeceeeeceaeeceuceceuseceeeesueessusessaeenees 54 11 8 Sample collection storage and preservation cccccecccceeececeeeeeeeeeeeeeeeeeees 59 TAH ETIOR CI CCS aaa a a ES 55 11 10 Prepare the SAMPIE ccccccccecccceeeceeeeeseeeeseeeeseeeeseeeeseeeeseeesseeeeseeeeseeeeaeeeas 56 11 11 Prepare Ihe test tubes ernan et ee a 57 11 12 Measure the toxicity of the sample dilutions nnnnnnnnnnnnennennnnneennnenenenenennnni 60 11 13 Show or send previous results ccccccceecccceseeeceeeeeceeeeeeeeseeseeseeseneeeseneeeeas 65 11 13 1 Description of screening luminescence reSults ccccceceeeeeeeeeeeeeeees 65 11 13 2 Description of LIMIT measure results
12. 6 2 2 Test suspension for D 1 values Prepare the test suspension for D1 values if the sample is probably non toxic to measure the sample toxicity using the highest possible sample concentration of 80 D 1 m 1 Remove the Diluent from the refrigerator Remove the cap from the bottle 4 Put 1 part stock suspension S at refrigerator temperature in to a clean reaction vessel using a pipette For example 0 1 mL S 2 0 mL D 2 Put 20 parts Diluent solution D at refrigerator temperature in to the reaction vessel using a pipette 5 Put the cap on the reaction vessel and shake to mix thoroughly Put a 1 20 label on the reaction vessel TT t 3 Remove the stock solution rehydrated reagent from the refrigerator Remove the rubber stopper from the reagent bottle 6 Put 50 parts Diluent solution D at refrigerator temperature in to a clean reaction vessel using a pipette 79 E 7 Put 1 part stock suspension S at refrigerator temperature in to a clean reaction vessel using a pipette For example 0 2mLS 10mLD 10 Set the 0 2 1 mL pipette to 0 2 mL 80 8 Put the cap on the reaction vessel and shake to mix thoroughly Put a 1 50 label on the reaction vessel 4 11 Put the end of the pipette in to a clean pipette tip B WO m O c OO u gt O 9 Put one half of the new empty test tubes in Row B and one half of the test tu
13. 9600 bps Off O O Off O Off O O O O O O O O Off Off O O O Off Off O O n n n n n n n n n n n n n n n n 20 f When Write is shown push PAPER FEED SW g Turn on the printer 7 If not using a DPU 414 thermal printer configure the printer Busy control XON XOFF 3 5 Connect the luminometer to a computer To connect the luminometer to a computer 1 Install the LUMISsoft software on the computer refer to Install LUMISsoft on the computer on page 21 2 Pull out the plug that is attached to the lanyard 3 Connect the RS232 serial interface cable to the luminometer 4 Connect the other end of the RS232 serial interface cable to the computer 3 6 Install LUMISsoft on the computer Install LUMISsoft on a computer with Windows 95 or greater by doing the instructions on the CD cover A shortcut for LUMISsoft is added to the desktop during installation In the lab LUMISsoft is used to automatically get LBT Measurement Luminescence procedure results from the luminometer during the test and put the values in to LUMISsoft LUMISsoft then does calculations according to ISO 11348 LUMISsoft is also used to send previous results that are saved on the luminometer to a computer as a text file The results can then be shown in graphical and tabular format using Microsoft Excel 97 or higher The user can also manually put test results shown on the luminometer in to LUMISsoft to do calcul
14. LUMIStherm NT 9 Put the test tubes in to the waste bag 107 12 13 Show or send previous results Measured luminescent procedure values are stored by an indicator counter M1 to Mx refer to Figure 12 The counter starts with M1 every time the storage is erased from the luminometer Figure 12 Example measurement luminescence results To show previous results on the luminometer for the Luminescent Bacteria Test LBT do the procedure in this section To send previous results to a computer Note At this stage the results can not be sent to the LUMISsoft 4 1 Do the steps in Connect the luminometer to a computer on page 21 2 Start LUMISsoft 3 In LUMISsoft select Transfer Options Interface Protocol Connect 4 Do the procedure in this section To send previous results to a printer do the steps in Connect the luminometer to a printer on page 19 and then do the procedure in this section To show or send previous results saved on the luminometer 1 Push ON green button to apply power to the luminometer The luminometer does built in tests that make sure that the electronics and software are operating correctly 2 When the built in tests are done push PROCEED The Main Menu is shown 3 Select Luminescent Bacteria Test and push ENTER The LBT Main Menu is shown 4 Select Previous Results and push ENTER 108 The Previous Results Menu is shown 5 Select Show Previous Measurements and push ENTER
15. Plaza No 9 A TR 06550 Cankaya ANKARA Tel 90 0 312 440 98 98 Fax 90 0 312 442 11 01 bilgi hach lange com tr www hach lange com tr HACH LANGE E P E 27 Avlidos str GR 115 27 Athens Tel 30 210 7777038 Fax 30 210 7777976 info hach lange gr www hach lange gr HACH LANGE SP ZO O ul Opolska 143 a PL 52 013 Wroctaw Tel 48 0 71 342 10 83 Fax 48 0 71 342 10 79 info hach lange pl www hach lange pl HACH LANGE S R L Str C minului nr 3 Sector 2 RO 021741 Bucure ti Tel 40 0 21 205 30 03 Fax 40 0 21 205 30 03 info hach lange ro www hach lange ro HACH LANGE D O O Fajfarjeva 15 SI 1230 Dom ale Tel 386 0 59 051 000 Fax 386 0 59 051 010 info hach lange si www hach lange si Section 17 Limited Warranty Hach Company warrants its products to the original purchaser against any defects that are due to faulty material or workmanship for a period of one year from date of shipment unless otherwise noted in the product manual In the event that a defect is discovered during the warranty period Hach Company agrees that at its option it will repair or replace the defective product or refund the purchase price excluding original shipping and handling charges Any product repaired or replaced under this warranty will be warranted only for the remainder of the original product warranty period This warranty does not apply to consumable products such as chemical reagents or consumable components
16. do the reading again Statistical research of each measurement range has shown that the standard deviation of the 0 2000 range is less than the standard deviaton of the 0 1000 range and that the precision at the 0 2000 range may be better In comparison studies of each range the phenol standardization check showed equal results to the expected 50 inhibition range Non polluted sea water samples enhance the signal give a higher light inhibition of approximately 40 As the sea water becomes more polluted the percentage inhibition increases towards 0 and then goes positive e g 10 Sea water which is very polluted gives a signal similar to that of fresh water which is very polluted e g 70 100 light inhibition 3 2 3 1 Eclox chemiluminescence test To show or change the measurement range for the Eclox chemiluminescence test 1 Push ON green button to apply power to the instrument The instrument does built in tests that make sure that the electronics and software are operating correctly 2 When the built in tests are done push PROCEED The Main Menu is shown 3 Select ECLOX and push ENTER The ECLOX Main Menu is shown 4 Select Set Up and push ENTER 16 The Set up Menu is shown 5 Select Set Measurement Range and push ENTER The current range is shown 6 Push YES to confirm 7 To change the range push CHANGE 8 Push STORE to save the change The Set up Menu is shown 3 2 3 2 Lum
17. if water gets in to the meter 2 Remove the battery cover of the luminometer with the Battery Cover Screw Tool 3 Remove the batteries from the luminometer and dispose of them in accordance with local operating procedures 4 Put four new batteries AA Alkaline in the luminometer Make sure the battery polarity is correct 5 Put the battery cover on the luminometer with the Battery Cover Screw Tool 6 Push ON green button to apply power to the luminometer 7 Do the pre deployment checks refer to section 3 2 1 Test the operation on page 14 Figure 13 Replace the luminometer batteries 2 Battery compartment 4 Battery Cover Screw Tool 13 3 2 Pocket Pal battery replacement pH and TDS 112 1 Turn the battery compartment cover located on the top of the tester to the left 4 turn with a coin 2 Remove the cover Put new batteries Everready E675E Duracell RM675 or Hach Item number 23678 00 in the tester Make sure the battery polarity is correct 3 Replace the cover Figure 14 Replace the Pocket Pal batteries L 1 Battery compartment cover 2 Batteries 113 Visit us at www hach com Section 14 Troubleshooting Table 4 Luminometer troubleshooting Change the measurement range to the 0O 2000 Detector overload range refer to section 3 2 3 Set the measurement range on page 16 Can not read the displa Change batteries refer to section 13 3 1 ae Luminometer battery replacemen
18. in Row A This corresponds to D values of 1 to 16 Figure 9 in the test as 0 5 mL of the sample dilution is added to 0 5 mL of the test suspension during the test in Row B and C Adding test suspension to the sample dilutions increases the sample dilutions in row A by a factor of two as final test concentration Note The test tubes in the higher Row A positions are more concentrated The pipette is moved from A9 to A4 higher concentration to lower concentration when making the dilution series so the pipette tip does not need to be replaced during this procedure D 1 D 16 A1 A2 A3 A4 Figure 9 Dilution series 9 dilutions D 1 values and higher D 12 D8 D6 D4 D3 D2 Gt A5 A6 A7 A8 A9 A10 2 NaCl 2 0 mL Sample 20 mt 2 NaCl 1 5 mL A4 A5 2 NaCl and sample 3 0 mL A6 A9 2 NaCl and sample 1 5 mL Sample 1 5 mL 93 Ow O g 1 00 ie ees 1 Put 10 empty test tubes 2 Set the 1 0 5 0 mL 3 Put the end of the pipette in to Row A of the pipette to 1 0 mL in to a clean pipette tip LUMIStherm P i 4 Put the tip of the pipette 5 Slowly dispense the 2 6 Set the 1 0 5 0 mL in to the 2 NaCl solution NaCl solution into the test pipette to 2 0 mL and slowly pull in 1 0 mL tube in position AY h 7 Put the tip of the pipette 8 Slowly dispense the 2 9 Set the 1 0 5 0 mL in to the 2 NaCl solution NaCl solution in to the test pipette to 1 5 mL and slowly pull in 2 0 mL tube
19. number 1730 00 Put the tube in to the comparator opening labelled Clear Sample Position 5 2 Fill one sample tube to the line underlining Item number 1730 00 with the sample This is approximately 15 mL If not using 1730 00 tubes fill to the line approximately 3 inches from the bottom of the tube A 5 Hold the comparator with the tube tops pointing to a window or light source Look through the openings in the front of the comparator When looking use care to not spill the samples from the unstoppered tubes 3 Put the tube that contains the water sample in to the comparator opening labelled Prepared Sample Position lt A 6 Turn the disc until a color match is seen The reading seen through the scale window is the apparent color in APHA Platinum Cobalt units 43 8 2 Color Test high range 1 Remove the lengthwise 2 Fill one tube tothe 5mL_ 3 Put the tube in the viewing adapter mark with the water top right opening of the sample comparator Ce Coe A A 4 Fill the other tube tothe 5 Hold the comparator up 6 Turn the disc until a 5 mL mark with clear water to alight source such asa_ color match is seen The Put the tube in the left window the sky or a lamp reading seen through the opening of the comparator and look through the scale window is openings of the multiplied by 5 to get the comparator apparent color in APHA Platinum
20. second tube DPD free chlorine reagent in the top right opening of powder pillow to the the color comparator second tube Complete the test and read the result within one minute of adding the powder 7 Hold the comparator up 8 Turn the color disc until 9 Read the mg L free to a light source and look the color matches in the chlorine in the scale through the openings in the two openings window front 40 7 3 Total chlorine procedure 0 3 5 mg L SS 1 Filla viewing tube tothe 2 Put the tube in the 3 Fill another viewing first 5 mL line with top left opening of the color tube to the first 5 mL sample water This is the comparator line with sample water blank gt nn 4 Add the contents of one 5 Swirl to mix Wait three 6 Put the second tube in DPD total chlorine reagent minutes the top right opening of powder pillow to the The result of the test must the color comparator second tube be read within six minutes of adding the powder A 7 Hold the comparator up 8 Turn the color disc until 9 Read the mg L total to a light source and look the color matches in the chlorine in the scale through the openings in the two openings window front 41 Visit us at www hach com Section 8 Color Test 8 1 Color Test low range 1 Put the lengthwise viewing adapter in the comparator SS 4 Fill the other sample tube with colorless water to the line underlining Item
21. store samples at 18 C for not longer than to 2 months Record preservation activities Before use defrost samples completely Homogenize the defrosted samples 82 12 8 Interferences Samples interferences can inhibit the light made by luminescent bacteria Interfering Interference levels and treatments substances Affects the viability of the bacterial reagent Chlorine is toxic to the bacteria To remove chlorine from a sample add one powder pillow of sodium thiosulfate Hach 1436369 dechlorination agent to 20 mL of sample and wait for 10 minutes High ete JEn Causes light inhibition that is not caused by toxicity consumption Sodium chloride Turbidity and color pH related light inhibition may occur if the pH is below 6 0 or above 8 0 The pH of the sample must be within 7 0 2 pH units of the standard A sodium chlorine NaCl concentrations of less than 15 g L or more than 50 g L or their osmolarity equivalents in a sample will cause osmosis related light inhibition The addition of solid NaCl to the sample 2 final concentration prevents osmosis related light inhibition of samples of low or unknown NaCl concentrations This biological test is strongly temperature dependent ISO 11348 requires that the test is done under temperature controlled conditions at 15 C using a appropriate thermostat i e LUMIStherm LTV053 Cause high bias results due to physical absorption or scattering of light Us
22. test suspension to the sample dilutions increases the sample dilutions in row A by a factor of two as final test concentration Note The test tubes in the higher Row A positions are more concentrated The pipette is moved from A9 to A2 higher concentration to lower concentration when making the dilution series so the pipette tip does not need to be replaced during this procedure D32 D24 D16 A1 A2 A3 A4 D 12 D8 D6 D4 D3 D2 AS A6 A7 A8 A9 A10 Figure 6 Dilution series 9 dilutions D 2 values and higher A2 A3 2 NaCl and sample 3 0 mL A4 A9 2 NaCl and sample 1 5 mL Sample 1 5 m 85 O mm O g 1 00 nieces 1 Put 10 empty test tubes 2 Set the 1 0 5 0 mL 3 Put the end of the pipette in to Row A of the pipette to 1 0 mL in to a clean pipette tip LUMIStherm i 4 Put the tip of the pipette Slowly dispense the 2 5 Set the 1 0 5 0 mL in to the 2 NaCl solution NaCl solution in to the test pipette to 1 5 mL and slowly pull in 1 0 mL tube in position AY h 6 Put the tip of the pipette 7 Slowly dispense the 2 8 Do steps 6 and 7 again in to the 2 NaCl solution NaCl solution in to the test to put 1 5 mL of 2 NaCl and slowly pull in 1 5 mL tube in position A8 solution in each test tube in positions A7 A6 A5 A4 A3 A2 and A1 Note Do not put 2 NaCl into the test tube in position A10 86 i E 9 Set the 1 0 5 0 mL 10 Put the tip of the pipette 11 Slowly dis
23. that a sample is not in the cell 2 Remove the black test tube holder from the cell Make sure that the cell is clean and free from debris 3 Put the black test tube holder in to the cell and close the cell lid 4 Push ON green button for several seconds to apply power to the instrument If the instrument does not energize replace the batteries refer to section 13 3 on page 112 14 10 11 12 13 14 15 The instrument does built in tests that make sure that the electronics and software are operating correctly Make sure that all tests show a PASS on the display If an error is shown on the display refer to Troubleshooting on page 115 Push PROCEED The Main Menu is shown Select either ECLOX or Luminescent Bacteria Test and push ENTER Either option can be selected for this procedure Check the battery level symbol at the top right corner of the display Make sure that at least two level bars are shown If two or more bars are not shown replace the batteries in the instrument and go back to step 4 Select System Tests and push ENTER The System Tests Menu is shown Push ENTER to select Check Signal Level The Signal Level screen is shown Push PROCEED to do a cell zeroing test When the instrument has passed the test the Signal Level screen is shown again Push and hold TEST Make sure that the signal level shown is above the minimum and below the maximum If the signal level is not above
24. that is created The more pollutant that is in the sample the less light that is created The light made by the sample water is compared to a pure water reference and the percentage inhibition of the light made is measured and made known e Arsenic Test measures the arsenic content of the water sample Arsenic is a common poison and industrial pollutant Arsenic is also in chemical warfare CW 10 agents such as Lewisite The result of this test can be read in mg L from a comparison chart Pesticide Nerve Agent Test gives a YES NO answer if pesticide nerve agents are in the water sample Chlorine Test measures how much free chlorine is in the water sample and gives the results in mg L Systems using monochloramines may choose to monitor total chlorine Chlorine is frequently used to disinfect water for human consumption The quantity of chlorine used must be carefully controlled and the free residual concentration of the chlorine in the water gives a useful means of monitoring the effectiveness of the water treatment done Chlorinated water can however cause damage to the Reverse Osmosis RO filter of water purification equipment and should not be used as a source water in a RO type of process Color Test a comparison test that compares the water sample with a calibrated gradient color disc The results are read in platinum cobalt Pt Co color units Color in water may be caused by the presence of natural metallic ions iron
25. the minimum contact the manufacturer for technical support Push and hold the back light button Make sure that the instrument display light comes on Push and hold QUIT for a few seconds The Systems Test Menu screen is shown Select Return to the ECLOX Main Menu or LBT Main Menu and push ENTER 3 2 2 Erase the results saved on the luminometer 1 Push ON green button for several seconds to apply power to the instrument The instrument does built in tests that make sure that the electronics and software are operating correctly When the built in tests are done push PROCEED The Main Menu is shown To erase all the Chemiluminescence Toxicity Test measurements select ECLOX and push ENTER 15 4 To erase all the Luminescent Bacteria Test measurements select Luminescent Bacteria Test and push ENTER 5 Select Set Up and push ENTER The Set Up Menu is shown 6 Select Clear All Measurements and push ENTER Push YES to confirm All saved measurements on the luminometer are erased 7 Push PROCEED The Set Up Menu is shown 3 2 3 Set the measurement range Set the luminometer measurement range to 0 1000 light units normal use or 0 2000 light units measurement of sea water samples If the measuring value is marked with an e g 1020 or the lumiometer shows Detector Overload the measurement is above the set measurement range If this occurs change the measurement range to 0O 2000 light units and
26. to 8 C If the temperature is higher the amount of initial light made by the bacteria will be lower 12 6 1 Prepare the stock suspension using the LCK491 reagent Prepare the stock suspension by adding the reconstitution solution to the freeze fried bacteria reagent The reconstitution solution rehydrates the bacteria reagent Reconstitution solution is specially made non toxic ultra pure water Do not make reconstitution solution or use substitutes The stock suspension can be kept in a refrigerator 8 C without being diluted with Diluent as long as the validity criteria are met Typically up to 4 hours The sensitivity spectrum of reactivated bacteria may shift as time elapses lf the reagent is to be used 90 minutes or more after reconstitution periodically monitor the performance of the reagent with a suitable standard to show changes in sensitivity This procedure is temperature sensitive Es n 1 Remove the 2 Remove the cap from 3 Remove the foil seal and luminescent bacteria test the reconstitution solution rubber stopper from the reagent from the freezer bottle reagent bottle Remove the reconstitution solution from refrigerator 75 g 1 00 4 Set the 1 0 5 0 mL 5 Put the end of the pipette 6 Put the tip of the pipette pipette to 1 0 mL in to a clean pipette tip in to the reconstitution solution and slowly pull in 1 0 mL w 7 Put the tip of the pipette 8 Put the rubber stopperin 9 Cool
27. warranty terms and no person is authorized to make any other warranties or representations on behalf of Hach Company Limitation of Remedies The remedies of repair replacement or refund of purchase price as stated above are the exclusive remedies for the breach of this warranty On the basis of strict liability or under any other legal theory in no event shall Hach Company be liable for any incidental or consequential damages of any kind for breach of warranty or negligence d 123 Limited Warranty 124 Appendix A Luminescent bacteria risks This appendix contains risk assessment information for Photobacterium fisheri synonym Vibrio fischeri manufactured by HACH LANGE GmbH in Germany The Luminescent Bacteria Toxicity Test reagent contains freeze dried or liquid dried Photobacterium fisheri bacteria Photobacterium fisheri luminescent bacteria are well known as non pathogenic and innocuous The origin of Photobacterium fisheri is the strain number DSM 7151 The bacteria are multiplied but not changed The bacteria are used as indicator organisms to identify the toxicity of environmental or chemical samples The Luminescent Bacteria Toxicity Test procedure has been standardized by ISO International Standard Organisation ISO 11348 1 2 3 A 1 Risk specifications Table 5 gives the risk specifications for Photobacterium fisheri Table 5Risk specifications DSM 7151 Vibrio fischeri Beijerinck 1889 Lehmann and Neumann 18
28. 0 Put the test tube in position B2 in to the black test tube holder in the luminometer cell 4 afl 99 SEZ 23 When the B2 measurement is done record the measuring value on a sheet of paper if the measuring value is shown but not saved to the luminometer saved to the computer or printed 24 Open the lid of the 25 Do steps 20 and 24 Aas fe a el luminometer again until o each test tube in Row B an Row C immediately after the Remove the test tube from All the test tubes in Row ube S measured the luminometer and put it B and C have been back in the LUMIStherm measured and recorded moving from left to right e g B2 C2 B3 C3 etc If testing D 1 values and higher 0 8 mL of the sample dilution from the test tube in position A2 has been added to the test tubes in position B2 and C2 If testing D 2 values and higher 0 5 mL of the sample dilution from the test tube in position A2 has been added to the test tubes in position B2 and C2 e 0 5 mL of the sample dilution from each test tube in Row A A3 and higher has been added to the test tubes in Row B and C that have the same position number e g from A4 to B4 and C4 105 12 12 Measure the light intensity of the test suspension after the sample dilutions are added Measure the light intensity of the test suspension luminescent bacteria after the sample dilutions are added according to ISO 11348 using this procedure The Lumine
29. 1 Overview The screening luminescence procedure or LIMIT measure procedure is done to identify if a sample is free of any inhibitory effects on the luminescent bacteria or if an inhibition is expected to make an inhibitory or risk assessment of the sample Therefore the user should measure dilutions of a sample and the percent inhibition of the dilution steps to get more information about the severity of the inhibitory effects In one run the sample is measured in three different concentrations 20 sample 50 sample and 80 sample The inhibitory effect of each sample dilution on the 49 luminescent bacteria is measured by the luminometer and is shown as percent inhibition Due to the nature of the simplified procedure and because the test is done at ambient temperatures the results may be different if compared directly with results for the same sample using the LBT measurement luminescence ISO 11348 procedure 11 2 Accuracy The error or standard deviation of the test is the sum of the error introduced to the test by all components the ambient and all manipulations The higher the degree of variation the higher the total error A Luminescent Bacteria Toxicity Test done strictly according to ISO 11348 has a better precision lower CV coefficient of variation than a LBT simplified luminescence screening procedure or LIMIT measure procedure under field conditions For screening measurements and LIMIT measurements the measure
30. 1 each 28229 99 EZ Arsenic Reagent 2 each 28230 99 EZ Arsenic Reagent Set Reagent 1 and 2 each 2823200 Lead acetate 100 mL each 14580 42 Reaction vessel arsenic each 28002 00 Sample cell 10mm each 26276 00 Test strips dual RG each 28001 50 Chlorine Test Color comparator box each 1732 00 Color disc DPD chlorine 0 3 5 mg L each 21988 00 Color viewing tube plastic with cap 4 pkg 46600 04 DPD free chlorine reagent powder pillows 100 pkg 14077 99 DPD total chlorine reagent powder pillows 100 pkg 14076 99 Caps plastic color viewing tubes 46600 04 4 pkg 46600 14 seater aes solution 50 75 mg L 2 mL 20 pkg 14268 20 Color viewing tube glass 6 pkg 1730 06 Stoppers glass color viewing tubes 1730 06 6 pkg 1731 06 Pesticide Test DPT test strips 50 pkg 28876 00 118 Replacement parts continued Description Qty Item number pH Pocket Pal pH tester each 44350 01 Singlet pH 4 01 and 7 00 10 each 27699 20 Battery 4 pkg 23678 00 TDS Pocket Pal TDS tester each 44400 01 180 ySlom NaCl 85 47 mg L as NaCl ea eran pepe erp cach 144004 1990 Siem NaCl 1000 mg L as NaCl et eile Battery 4 pkg 23678 00 Luminescent Bacterial Test satel va reagent freeze dried vials for 50 ml 12 pkg LCK491 Luminescent Bacteria Accessories Kit includes case reconstitution solution dilution solution 2 NaCl solution NaCl solid in a bottle with a dosing spoon plastic test tubes for Eclox reaction vessels
31. 16 60 To measure the toxicity of the sample dilutions 1 Push ON green button for several seconds to apply power to the luminometer When the built in tests are done push PROCEED The Main Menu is shown Select Luminescent Bacteria Test and push ENTER Select Measure and push ENTER To do the screening luminescence procedure a Select Screening Luminescence and push ENTER b Select one option that is shown To measure luminescence and save the results on the luminometer select Screen and Save and push ENTER To measure luminescence and manually record the measuring values on paper select Screen without Saving and push ENTER To measure the luminescence and send the results to a PC start LUMISsoft on the computer start the test on LUMISsoft and when Please select LSoft at the luminometer is shown select Measure Luminescence and Send to PC and push ENTER The luminometer must be connected to a computer refer to Connect the luminometer to a computer on page 21 To measure the luminescence and print the results on a printer select Screening and Send to Printer and push ENTER The luminometer must be connected to a printer refer to Connect the luminometer to a printer on page 19 To do the LIMIT measure procedure a Select LIMIT Measure and push ENTER b Select Set LIMIT Value and push ENTER c Push CHANGE to set the LIMIT value d Push STORE to save the LIMIT value shown e Select one option that is
32. 5 0 mL 3 Put the end of the pipette to Row A of the pipette to 1 0 mL in to a clean pipette tip LUMIStherm g 1 50 h 4 Put the tip of the pipette 5 Slowly dispense the 2 6 Set the 1 0 5 0 mL in to the 2 NaCl solution NaCl solution into the test pipette to 1 5 mL and slowly pull in 1 0 mL tube in position A3 90 7 Put the tip of the pipette in to the 2 NaCl solution and slowly pull in 1 5 mL 8 Slowly dispense the 2 NaCl solution in to the test tube in position A2 10 Set the 1 0 5 0 mL pipette to 2 0 mL pull in 2 0 mL 13 Set the 1 0 5 0 mL pipette to 1 5 mL 14 Put the tip of the pipette in to the sample and slowly pull in 1 5 mL 11 Put the tip of the pipette in to the sample and slowly 9 Do steps 7 and 8 again to put 1 5 mL of 2 NaCl solution in to the test tube in position A1 12 Slowly dispense the sample in to the test tube in position A3 15 Slowly dispense the sample in to the test tube in position A4 91 16 Do steps 14 and 15 again to put 1 5 mL of the sample in to the test tube in position A2 92 HRS MIN 17 Keep the dilution series at 15 C for at least 5 minutes to correct the temperature 12 10 3 Prepare a 9 dilution series D 1 values and higher To make a 9 dilution series according to ISO 11348 of D 1 sample values and higher do this procedure This procedure makes dilutions ranging from undiluted to a dilution ratio of 1 8
33. 8 Put the tip of the pipette in to the sample and slowly pull in 0 2 mL as 21 Put the tip of the pipette in to the sample and slowly pull in 0 5 mL 59 22 Slowly dispense the 23 Set the 0 2 1 0 mL 24 Put the tip of the pipette sample in to the test tube pipette to 0 8 mL in to the sample and slowly in position 3 pull in 0 8 mL 25 Slowly dispense the 26 Remove the pipette tip sample in to the testtube in from the pipette and put in position 4 the waste bag Put the pipette in the storage case 11 12 Measure the toxicity of the sample dilutions The Luminescent Bacteria Toxicity Test is a biological test method and the result is therefore strongly temperature dependent Record the temperature at which the test was done The results of tests done at different temperatures can not be compared directly A non toxic reference is added to the test suspension during the test and measured The reference measurement is used to compensate for changes in light levels from the luminescent bacteria The light levels change with time In some instances if reconstitution is done at the optimum temperature and the test is carried out at 20 C the initial light made by the bacteria can be more than 1000 Eclox light units This causes the error Detector Overload If an error occurs change the measurement range from the 0 1000 range to the 0 2000 range and do the readings again refer to Set the measurement range on page
34. 96AL Bacteria by DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH Braunschweig Germany Name Vibrio fischeri Beijerinck 1889 Lehmann and Neumann 1896AL DSMZ number 7151 NRRL B 11177 ATCC 49387 ATCC Number 49387 Restrictions Risk NRRL Number B 11177 Group 1 Organism Photobacterium phosphoreum harmless Cohn Beijerinck bacteria Designations NRRL B 11177 Depositors NRRL Biosafety Level 1 ATCC American Type Culture Collection NRRL ARS Culture Collection Northern Regional Research Laboratory Strain number Biosafety Level 125 A 2 Biosafety Level 1 information Biosafety Level 1 practices safety equipment and facility design and construction are appropriate for undergraduate and secondary educational training and teaching laboratories and for other laboratories in which work is done with defined and characterized strains of viable microorganisms that are not known to consistently cause disease in healthy adult humans Bacillus subtilis Naegleria gruberi infectious canine hepatitis virus and exempt organisms under the NIH Recombinant DNA Guidelines are representative of microorganisms meeting these criteria Many agents that are not normally associated with disease processes in humans are however opportunistic pathogens and may cause infection in the young the aged and immunodeficient or immunosuppressed individuals Vaccine strains that have undergone multiple in vivo passage
35. Cobalt Units 44 Section 9 Pocket Pal pH Tester 9 1 Use and care of the tester 1 Push ON OFF onetime 2 Remove the protective 3 Put the bottom of the to apply power to the cap from the bottom of the tester 2 5 to 8 9 cm tester tester 1 0 to 3 5 in in to the sample v SY lil KY M A a 4 Gently mix the sample 5 Clean the bottom of the 6 For faster response and for several seconds with tester with water Put the longer test life put several the tester When the digital cap on the tester drops of deionized water in display is stable read the the protective cap to keep DH value the glass bulb from drying between use Note Soak the electrode tip in tap water for a few minutes each week to extend the life of the electrode Note If the pH readings become erratic replace the batteries refer to section 13 3 2 on page 112 Note Potassium chloride which is used as reference solution electrolyte may attach to the tester as a white precipitate Although the precipitate is normal and does not affect the performance it may be removed with a damp cloth or tissue 45 9 2 2 point calibration 1 Make a pH 7 00 anda pH 4 00 or 10 00 buffer using the Singlet pouches supplied with the kit 46 2 Measure the pH using the tester Calibration p gy mers pA R f Y Trimmer T Tool a Cd 3 If necessary change the calibration trimmer using the supplied trimme
36. D 28868 88 Eclox Rapid Response Test Kit User Manual March 2010 Edition 3 Hach Company 2004 2006 2010 All rights reserved Printed in the U S A mm sm ej kt Visit us at www hach com Table of Contents Section 1 Specifications cece cc eccecseeeeceeeeecseeeeceeeceeseeeeseeeessaeeessaeeessaes 7 Section 2 General information cccccccccccccccceececceececeeeceeseeeeeseueeeseeeeseeeessaes 9 2 Vi Dale by INOA 35 sete eel eee ent tana ee eee ee 9 2 1 1 Use of hazard information ccccccccccceseececceeceeseeeeeceeeeeseeceeseeeeesaeeeesaees 9 2 2 PROOUCE OVEIVICW cacen he ee ee ee eee 10 2 2 1 Test descriptions ccc ceeecseeceseecee cece ceseueeseeeceaeessaeecsaeesseessueesseeseas 10 ZA A TESTS IO rire he eee ee ee ee 12 2 3 Unpack the instrument ccccccccceececeeeeceeeeseeeeseeeeseeeeseeeeseaeeseuseseeseseeeeeeaeesees 12 Section 3 Luminometer seis535 desi dese atecesvosdecuudeeesiepuccactesusa fan ead deadee sauccasde cee vewecaeaeees 13 On TOVE OW oxic aetna eee ee 13 3 2 Prepare the luminometer fOr USC c ccccccseececceeeeeceeececeeeeseeeeesaeeesseeesaneees 14 3 21 TESTING ODGlallOn 2c eee ee ere 14 3 2 2 Erase the results saved on the lUmMinometel ccccseeeeeeeeeeeeeeeeeees 15 3 2 3 Set the measurement range ccceccccseceeceeeeeceeeeeceeceeseueeeseaeeessseeeeaes 16 3 3 Change the default settings cccceeccceeseeeeeec
37. Show Previous LIMITs and push ENTER 67 68 The Previous LIMITs Menu is shown To show all or send all of the results saved on the luminometer select one option To show the results on the luminometer select Show all R1 to Rx and push ENTER To send the results to the computer select Send all R1 and Rx to PC and push ENTER To send the results to the printer select Send all R1 to Rx to Printer and push ENTER To show or send a specific range of results saved on the luminometer select one option To show the results on the luminometer select Show selection and push ENTER To send the results to the computer select Send selection to PC and push ENTER To send the results to the printer select Send selection to Printer and push ENTER If an option in step 7 was selected select the data to be recalled a Select the starting value in the From field Push SELECT to change the starting value Then push PROCEED b Select the ending value in the To field Push SELECT to change the ending value Then push SHOW Push PROCEED to show more results Section 12 Luminescent Bacteria Toxicity Test according to ISO 11348 part 3 The Luminescent Bacteria Toxicity LBT Test uses the luminometer Before doing the procedure read section 3 1 Overview on page 13 and do the procedures in section 3 2 Prepare the luminometer for use on page 14 This chapter describes the LBT Test measurement luminescence procedure and
38. and manganese peat materials plankton weeds and industrial wastes Total Dissolved Solids TDS Test measures the level of dissolved solids in the water sample TDS and the conductivity of a sample are related The TDS is approximately 0 7 of the conductivity result uS cm pH Test measures the pH level of the water sample Luminescent Bacteria Toxicity Test optional a biotest that measures the toxicity of environmental samples Toxicity is a biological or biochemical sum parameter that can not be measured by chemical analysis Toxicity is a measure of the effect of a sample on living organisms biological systems and enzymes Other biotests such as fish daphnia and algae tests are more complex and because other biotests use higher living organisms are also controversial In the practice of environmental analysis the Luminescent Bacteria Toxicity Test has shown to be fast simple reliable and sensitive The Luminescent Bacteria Toxicity Test uses natural bacteria that make light Toxic samples decrease the amount of light the bacteria make The more toxic the sample the less light the bacteria make The amount of light that is made by the bacteria after exposure to a sample is compared to the amount of light made by the bacteria after exposure to a control to identify the percent inhibition value of the sample The control contains no sample but a non toxic reagent blank 2 NaCl solution The reagent set is sold independent
39. and one test with two samples or sample dilutions The results of the first test are indicated as R1 with S1 S2 and S3 The results of the second tests are indicated as R2 with S1 and S2 Figure 4 Example of screening luminescence results 65 11 13 2 Description of LIMIT measure results The LIMIT measure procedure results are recorded the same as the screening luminescence results The only difference is that the LIMIT measure results include a column that shows whether the percentage inhibition calculated for each sample measurement is above the LIMIT value or below the LIMIT value set by the user as shown in Figure 5 Figure 5 Example LIMIT measure results 11 13 3 Show or send screening luminescence results To show or send previous results saved on the luminometer for the screening luminescence procedure 1 Push ON to apply power to the luminometer The luminometer does built in tests that make sure that the electronics and software are operating correctly 2 When the built in tests are done push PROCEED The Main Menu is shown 3 Select LUMINESCENT BACTERIA TEST and push ENTER The Luminescent Bacteria Test Main Menu is shown 4 Select Previous Results and push ENTER The Previous Results Menu is shown 5 Select Show Previous Screenings and push ENTER The Previous Screenings Menu is shown 6 To show all or send all of the results saved on the luminometer select one option 66 9 To show the r
40. ath viewing adapter each 24122 01 Luminometer each 28870 00 Pesticide test clip each 28877 00 Pipet liquid transfer 1000 uL Blue each 28873 00 Pipet liquid transfer 100 uL Yellow each 28871 00 Pipette liquid transfer 100 uL tip set each 28872 00 Serial comms lead cable luminometer each 28882 00 Stopper each 14480 7Y Test record sheets 15 pkg 28883 00 Waste bottle 250 mL each 28884 00 Chemiluminescence Toxicity Test Chemiluminescent Reagent Set 100 tests each 94 9004 Cuvette and 1000 uL Pipet Set 2 28874 00 Cuvette and 1000 uL Pipette Tip Set each 2887400 Cuvette for Eclox 500 pack each 30 0015 Cuvette and Pipette Tip Set 25 tests ach 10 9009 Cuvette and Pipette Tip Set 50 tests each 10 9011 100 uL Pipet Tip Set 2 28872 00 Optional Mustard Gas Test 50 tests each 10 9004 Pesticide Nerve Agent Test coupons 25 tests each 28876 00 117 Replacement parts continued Description Qty Item number Chemiluminescence Test Kit for 50 tests includes CT ee ee ee pre conditioner Eclox Replacement Reagent Set includes 50 chemiluminescent reagent sets 25 pesticide nerve agent tests 50 free chlorine 50 total chlorine 100 Hach Arsenic Tests 10 pH buffer Singlets 4 01 from Hach and 10 pH each 28869 00 buffer Singlets 7 00 from Hach Pentre Revyontiate as cach 90 9003 Arsenic Test Cap Arsenic Test Kit each 49348 00 Cotton balls 2572 01 EZ Arsenic Reagent
41. ation on the Luminescent Bacteria Toxicity Test 70 12 2 Accuracy The error or standard deviation of the test is the sum of the error introduced to the test by all components the ambient and all manipulations The higher the degree of variation the higher the total error A Luminescent Bacteria Toxicity Test done strictly according to ISO 11348 has a better precision lower CV coefficient of variation than a simplified screening test under field conditions The total error for the test is typically lower than 20 71 12 3 Thermostat and PC software requirements ISO 11348 states that the measuring luminometer must have a 15 C temperature controlled measuring well The Eclox does not have a temperature controlled measuring cell According to ISO 11348 optional accessories that should be used in the lab include e LTV053 LUMIStherm 230V thermostat to 15 C e LZV093 LUMISsoft 4 PC software 72 12 4 Reagent description The Luminescent Bacteria Toxicity Test reagent contains living luminescent bacteria that have been grown under optimal conditions harvested and lyophilized freeze dried The reagent is a freeze dried preparation of a specially selected strain of the marine bacterium Vibrio fischeri formerly known as Photobacterium phosphoreum NRRL number B 11177 A vial of reagent contains roughly one hundred million test organisms Refer to section Appendix A Luminescent bacteria risks on page 125 for bacteria
42. ations 21 22 Section 4 Chemiluminescence Toxicity Test The Chemiluminescence Toxicity Test uses the luminometer Before doing the Chemiluminescence Toxicity Test read section 3 1 Overview on page 13 and do the procedures in section 3 2 Prepare the luminometer for use on page 14 4 1 Overview The Chemiluminescence Toxicity Test and Luminescent Bacteria Toxicity Test both show the inhibitory effects of a sample However the Chemiluminescence Toxicity Test reagents are more rugged than the Luminescent Bacteria Toxicity Tests reagent and can be used under conditions where the Luminescent Bacteria Toxicity Tests reagent can not be used The Chemiluminescence Toxicity Test reagents are stable for months even if stored under higher ambient temperatures up to 40 C Table 1 The Luminescent Bacteria Toxicity Tests reagent can not be stored under those conditions 4 2 Prepare the reagents for luminometer calibration and sample testing Prepare the chemiluminescence test CT Reagents 2 and 3 at the beginning of deployment The chemiluminescence test CT Reagents 2 and 3 are temperature sensitive and degrade at high temperatures For long term storage store the reagents in their stable forms On the first day of testing prepare the reagents for routine use Diluted reagents are stable for 72 hours The life of the reagents is longer if the reagents are kept cool e g in a refrigerator and in a dark place Before use let the r
43. bes in Row C of the LUMIStherm Note The LUMIStherm should be operating at 15 C uy 12 Put the tip of the pipette in to the reaction vessel that contains the 1 20 test suspension and slowly pull in 0 2 mL of the test suspension 13 Slowly dispense the test suspension in to the test tube in position B1 my 16 Put the tip of the pipette in to the reaction vessel that contains the 1 50 test suspension and slowly pull in 0 5 mL of the test suspension SEC HRS MIN 19 Cool the filled test tubes in the LUMIStherm at 15 C for 15 minutes 15 Set the 0 2 1 mL pipette to 0 5 mL 14 Do step 12 and 13 again until the test tubes in position C1 B2 and C2 contain 0 2 mL of test suspension 18 Do step 16 and 17 again until each test tube in Row B and Row C position 3 and higher contains 0 5 mL of test suspension 17 Slowly dispense the test suspension in to the test tube in position B3 N JU 20 Remove the pipette tips from the pipettes and put in the waste bag Put the pipettes in the storage case 81 12 7 Sample collection storage and preservation The test can be used with samples of municipal and industrial waste water aqueous eluates from soil and waste aqueous solutions of pure chemicals and with surface well and water of other sources Collect samples in clean glass bottles Keep samples in the dark at 0 to 5 C for no longer than 2 days Freeze and
44. contains the procedure steps Use the LBT Test measurement luminescence procedure if the test needs to be done according to ISO 11348 part 3 The Eclox LBT Test measurement luminescence procedure meets the criteria of validation of ISO 11348 3 69 12 1 Overview The test criterion is luminescence which is measured after a contact time of 15 or 30 minutes optionally 5 minutes at 15 C taking in to account a correction factor fK The correction factor is a measure of intensity change of control samples during the exposure time refer to the ISO standard procedure The luminometer measurements are in relative light units The luminometer measurements are used by the LUMISsoft computer program or custom made calculations to calculate percent inhibition LID EC20 and EC50 values e Percent inhibition the percentage of light made by the bacteria that is inhibited by the sample The higher the percentage inhibition of the light emission the more harmful the sample is to the bacteria and the higher the toxicity level of the sample e LID first dilution value of a sample that causes less than 20 inhibition The higher the LID the more harmful the sample is to the bacteria e EC20 or EC50 the concentration of a sample that causes exactly 20 or 50 inhibition The lower the EC value the more harmful the sample is to the bacteria The linear measuring range is between 10 and 90 inhibition Refer to ISO 11348 for more detailed inform
45. ction 3 1 Overview on page 13 e Do the procedures in section 3 2 Prepare the luminometer for use on page 14 e Print color copies of the Screening Luminescence Results Sheet to use in the field refer to page 64 from www Hach com This chapter describes the LBT screening luminescence procedure and LIMIT measure procedure and contains the procedure steps The screening luminescence procedure and LIMIT measure procedure are used in the field or in an emergency situation when a rapid assessment of the inhibitory effects of a sample is necessary The screening luminescence procedure and LIMIT measure procedure are a simplified test procedure that uses the same reagents according to ISO 11348 but at ambient conditions The LBT screening luminescence procedure and LIMIT measure procedure are done the same with two exceptions e Different options on the luminometer are selected to measure the sample dilutions e Different options on the luminometer are selected to show or send results e A LIMIT value is set by the user using the luminometer for the LIMIT measure procedure A column is added to the LIMIT measure test results that shows whether the percent inhibition measured for each sample dilution is above or below the LIMIT value percent inhibition set by the user on the luminometer Do the LBT screening luminescence procedure to do a toxicity screening measure Do the LBT LIMIT measure procedure to do a toxicity limit measure 11
46. e co uk www hach lange co uk DR BRUNO LANGE AG Juchstrasse 1 CH 8604 Hegnau Tel 41 0 44 9 45 66 10 Fax 41 0 44 9 45 66 76 info hach lange ch www hach lange ch DR LANGE NEDERLAND B V Laan van Westroijen 2a NL 4003 AZ Tiel Tel 31 0 344 63 11 30 Fax 31 0 344 63 11 50 info hach lange nl www hach lange nl HACH LANGE S R L Via Riccione 14 l 20156 Milano Tel 39 02 39 23 14 1 Fax 39 02 39 23 14 39 info hach lange it www hach lange it 121 Contact Information HACH LANGE S L U Edif Arteaga Centrum C Larrauri 1C 2 PI E 48160 Derio Vizcaya Tel 34 94 657 33 88 Fax 34 94 657 33 97 info hach lange es www hach lange es HACH LANGE S R O Le ansk 2a 1176 CZ 141 00 Praha 4 Tel 420 272 12 45 45 Fax 420 272 12 45 46 info hach lange cz www hach lange cz HACH LANGE 8 Kr Sarafov str BG 1164 Sofia Tel 359 0 2 963 44 54 Fax 359 0 2 866 04 47 info hach lange bg www hach lange bg HACH LANGE E N E AuAidoc 27 GR 115 27 A va TnA 30 210 7777038 Fax 30 210 7777976 info hach lange gr www hach lange gr 122 HACH LANGE LDA Av do Forte n 8 Fracgao M P 2790 072 Carnaxide Tel 351 214 253 420 Fax 351 214 253 429 info hach lange pt www hach lange pt HACH LANGE KFT Hegyalja ut 7 13 H 1016 Budapest Tel 36 06 1 225 7783 Fax 36 06 1 225 7784 info hach lange hu www hach lange hu HACH LANGE SU ANALIZ SISTEMLERI LTD STI Hilal Mah 75 Sokak Arman
47. e color correction cuvettes accessories in a separate test according to ISO 11348 or dilute the samples i e 25 or 50 before testing in the screening measure to remove the interference 83 12 9 Prepare the sample To prepare the sample for testing 1 Ifthe sample is turbid either e Filter the sample with a modified polysulfone filter Before using other filter materials test the filter material with 2 NaCl first to make sure that the filter material can be used with the Luminescent Bacteria Toxicity Test Check the acceptable filters in the ISO method Note Do not use a cellulose nitrate or a cellulose acetate filter The use of cellulose nitrate or cellulose acetate filters can cause light inhibition that is not caused by the sample e Let the sample sediment for 1 hour or e Centrifuge the sample e g 10 minutes at 5 000 g 2 Check the pH level Adjust the sample to pH 6 to 8 using HCI or NaOH Use a strength of HCI or NaOH that does not change the volume of the sample by more than 5 in total 3 Add solid NaCl to the sample until the concentration in the sample is 2 w v For example weigh out 0 3 g of NaCl and dissolve it in 15 mL of sample or dissolve one spoon of solid NaCl LCX058 in 7 mL of sample The concentration of salt in the test should not exceed 35 g L Note Do not add NaCl to the sample if the salt concentration of the sample is more than 20 g L guide value conductivity of 35 mS cm Note T
48. eagents get to ambient temperature Table 1 Chemiluminescence test reagent stability Reaaent Refrigerated in Raised Temperatures 3 a dark place 40 C 23 4 2 1 Prepare CT Reagent 2 AOol Oe 1 Remove the CT 2 Carefully put all of the 3 Put the caps back on Reagent 2 buffer and CT CT Reagent 2 buffer in to the bottles Shake the CT Reagent 2 caps the CT Reagent 2 bottle Reagent 2 bottle for 30 seconds Let dissolve for Note Do not open the bottles 40 minutes before use in heavy winds The reagent in the CT Reagent 2 is small Note Do not touch the reagent 4 2 2 Prepare CT Reagent 3 _ _ Gla 1 Remove the CT 2 Push the end of the pipet 3 Push in the operating Reagent 3 concentrate and in to a clean 100 uL yellow button on the top of the CT Reagent 3 caps pipet tip and remove from pipet to the stop the box Note Make sure that the batch number for CT Reagent 3 is the same as the batch number used for CT Reagent 2 24 gt 0 L Pipette 4 Put the tip in the CT Reagent 3 concentrate 1 cm below the surface Slowly release the operating button to pull in the concentrate A N a 7 Put the cap on CT Reagent 3 Turn over the CT Reagent 3 bottle several times to mix the solution Note An ice chest can be used in the field to extend the life of the reagent ex 0 L Pipette 5 Put the tip in to CT Reagent 3 and dispense the liquid by gent
49. ease the operating button slowly to pull in the deionized water in to the pipette Touch the pipette tip against the side of the deionized water bottle to remove any drops from the outside of the tip Place the pipette tip in to the cuvette and dispense the liquid in to the cuvette by gently pushing in the operating button to the stop 16 17 18 19 20 21 22 23 24 25 26 27 28 Put the pipette tip in to the cuvette and remove the pipette from the cuvette to remove any drops from the outside of the tip Remove the lids from the CT Reagents 1 2 and 3 Put a yellow pipette tip on to the yellow pipette Put 100 uL of CT Reagent 1 2 and 3 in to the cuvette using the pipette Use a new pipette tip for each reagent Open the lid of the luminometer Lift the cuvette from the holder and gently tap the cuvette two times to mix the solution Immediately put the cuvette in to the luminometer cell and close the lid Push PROCEED The luminometer automatically starts measuring After four minutes the screen count down timer displays DONE When the measurement is complete remove the cuvette from the luminometer Put the solution in the cuvette in to the waste bottle Put the cuvette in to the waste bag If the reference is between 300 and 900 the calibration is complete If the reference is not between 300 and 900 push PROCEED and do the calibration procedure again Note New reagents may give a signal
50. ectly When the built in tests are done push PROCEED The Main Menu is shown Select Luminescent Bacteria Test and push ENTER Select Set Up and push ENTER The Set up Menu is shown To show or change the waiting time a Select Set Waiting Time and push ENTER The current settings are shown b To change the waiting time push CHANGE c Push STORE to save the change The Set up Menu is shown To show or change the measuring time a Select Set Measuring Time and push ENTER The current settings are shown b To change the measuring time push CHANGE c Push STORE to save the change The Set up Menu is shown 3 4 Connect the luminometer to a printer Luminometer measurements can be sent to a printer either during the test or after the test is done To connect the luminometer to a printer 1 2 3 Pull out the plug that is attached to the lanyard If using a DPU 414 thermal printer turn off the printer Connect the RS232 serial interface cable to the luminometer 19 4 Putan adapter DB9 3 wires male male 2 3 3 2 5 5 Cross over not straight on the other end of the RS232 serial interface cable 5 Connect the RS232 serial interface cable to the printer 6 If using a DPU 414 thermal printer d Configure the printer refer to the printer manual for more information Input Serial DIP SW1 DIP SW2 DIP SW3 e When Continue is shown push ON LINE SW Busy control XON XOFF On
51. eeeeeeeeseeeeeeeeeseeeeeeseeeeeeeseaeeeeeeaas 18 3 0 SETING LCD COM MAST aceted oars eats pened sea sald odes MoO tessa a 18 3 3 2 Set the waiting time and measuring tIME cece eeeceeceeeeeeeeeeeeeeeeeeees 18 3 4 Connect the luminometer to a Printer cccceceeceeceeceeceeeeeseeeeeeseeeeeseeeeeees 19 3 5 Connect the luminometer to a COMPUTE ccc eceecceceeececeeeeeeeeeeeeseeeeeseeeeeees 21 3 6 Install LUMISsoft on the computer ccc ecccceeeeeceeeeeceeeceeseeeeeseeeeessaeeeesaaeees 21 Section 4 Chemiluminescence Toxicity TeSt cccccsecccesseeeeseaeeeeeeeaeeeees 23 od 12 V1 ae nee re ee eee eee 23 4 2 Prepare the reagents for luminometer calibration and sample testing 23 4 2 1 Prepare CT Reagent 2 cccccccssseeccccesseecceaeeceescesecessueeeeesauseeeesaaeeees 24 4 2 2 Prepare Cl Reagent 3 merisier a aa iadaa aiaia 24 4 3 Calibrate the luminometer nn nnnnnnunennnnennneninnnnnnsnrrnerrennnrrnnrrrerrrrrnrennrrnrnernnni 26 4 4 Measure pollutants in the water SAMPIE cccccccecceseeeeseeeeseeeeseeeesaeeeeaeeeaes 28 4 5 Show the previous results ccccccsseccccsssceceeececeeececeeeeceseuseessueeesseeeeseneeesaues 31 4 6 Send previous results to a COMPUTES ccc ceecccseeeeeeeeeeeeeeeeeseeeeeseeeeeseeeeeeas 31 section 5 EZ Arsenic Test Kit sncos26 uc ek el A 33 5 1 EZ Arsenic 0 500 ppb 0 10 25 50 100 250 500 eeeeee nee
52. esults on the luminometer select Show all R1 to Rx and push ENTER To send the results to the computer select Send all R1 and Rx to PC and push ENTER To send the results to the printer select Send all R1 to Rx to Printer and push ENTER To show or send a specific range of results saved on the luminometer select one option To show the results on the luminometer select Show selection and push ENTER To send the results to the computer select Send selection to PC and push ENTER To send the results to the printer select Send selection to Printer and push ENTER If an option in step 7 was selected select the data to be recalled a Select the starting indicator in the From field Push SELECT to change the value Then push PROCEED b Select the ending indicator in the To field Push SELECT to change the value Then push SHOW Push PROCEED to show more results 11 13 4 Show or send LIMIT measure results To show or send previous results saved on the luminometer for the LIMIT measure procedure 1 Push ON to apply power to the luminometer The luminometer does built in tests that make sure that the electronics and software are operating correctly When the built in tests are done push PROCEED The Main Menu is shown Select LUMINESCENT BACTERIA TEST and push ENTER The Luminescent Bacteria Test Main Menu is shown Select Previous Results and push ENTER The Previous Results Menu is shown Select
53. ette 2 to 3 times to mix the sample dilution h mP 4m _ _ a 20 Pull the solution in A8 in to the pipette 2 to 3 times to mix the sample dilution 23 Pull in 1 5 mL of solution from A4 and put it in to A2 using the pipette Pull the solution in A2 in to the pipette 2 to 3 times to mix the sample dilution mM Ci hh ee HRS MIN SEC 24 Keep the dilution series at 15 C for at least 5 minutes to correct the temperature p A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 Figure 7 Before the dilution series is started 9 dilutions D 2 and higher 1 Sample 2 2 NaCl solution 12 10 2 Prepare a 3 dilution series D 2 values and higher To make a 3 dilution series according to ISO 11348 of D 2 sample values and higher do this procedure This procedure makes dilutions ranging from undiluted to a dilution ratio of 1 2 in row A This corresponds to D values of 2 to 4 Figure 8 in the test as 0 5 mL of the sample dilution is added to 0 5 mL of the test suspension during the test in Row B and C Adding test suspension to the sample dilutions increases the sample dilutions in row A by a factor of two as final test concentration 89 Figure 8 shows the contents of the test tubes in Row A at the end of this procedure Figure 8 Dilution series 3 dilutions D 2 values and higher 1 Sample 2 2 NaCl solution A OOOO g 1 00 123 4 5 1 Put 4 empty testtubesin 2 Set the 1 0
54. gent Test 6 1 Pesticide Nerve Agent procedure 1 Remove one pesticide strip from the storage case Open the foil packet on the notched side Remove the contents Keep the strip and the foil but put the wadding in the bag 2 The pesticide strip has a white disc at one end anda larger pink disc at the other end Fold back the protective film covering the white disc only 3 Put the white disc in the beaker that contains the sample water for at least one minute 4 Remove the pesticide strip from the sample beaker 5 Remove the protective film covering the pink disc Fold the strip in half along the perforations and push the white disc against the pink disc 6 Put the strip in the pesticide clip and put the Strip clip back in to the foil packet Keep the foil packet warm by holding it under the armpit outside of clothes for three to four minutes 37 7 Open the strip and look 8 There are two possible 9 Do the test again if a at the color of the smaller results positive result is seen or disc For the best results compare with a test from hold the strip against A white disc indicates a known clean water something white so the POSTIVE pesticides or sample color developmentis easier nerve agent are present to see A blue disc matching blue or darker blue than larger disc indicates NEGATIVE no pesticide or nerve agent present nfl Ot 10 Record the results
55. h d Push SELECT again on the last selected graph number to select multiple graphs 4 6 Send previous results to a computer To send previous results to a computer 1 2 3 Do the steps in Connect the luminometer to a computer on page 21 Start LUMISsoft In LUMISsoft select Transfer Options Interface Protocol Connect 31 32 Push ON green button for several seconds to apply power to the luminometer The luminometer does built in tests that make sure that the electronics and software are operating correctly When the built in tests are done push PROCEED The Main Menu is shown Select ECLOX and push ENTER Select Previous Results and push ENTER The Previous Results Menu is shown Select Download to PC and push ENTER Section 5 EZ Arsenic Test Kit CAUTION Hydrogen and arsine gases are generated during this test Work in a well ventilated area away from open flames and other sources of ignition Review the Material Safety Data Sheets before handling any chemicals 5 1 EZ Arsenic 0 500 ppb 0 10 25 50 100 250 500 1 Lift the flap on the black 2 Fill the reaction vessel 3 Add the contents of cap and put a test strip in with sample water to the one Reagent 1 and one to the groove The reactive top edge of the line Reagent 2 powder pillow pad should be against and 50 mL to the reaction vessel over the small opening Hold in place by pushing See Interferences on page 34 the flap d
56. he salt content of the sample should not exceed 50 g L This corresponds to a conductivity of about 70 mS cm without taking other conductive compounds in to account Solid NaCl is used to change the sample osmolarity to a value that is correct for the marine bacterium used in the test 4 Ifthe sample has a high toxicity carry out a preliminary dilution of the sample with 2 NaCl solution Select a preliminary dilution from the levels 1 2 1 4 1 8 1 16 etc to make sure of a continuous dilution series using the dilution procedure of the manufacturer 84 12 10 Prepare the dilutions series Prepare the sample dilutions series using one of the procedures in this section The sample dilutions are added to the test suspension later to identify the percent inhibition of each sample dilution A non toxic reference is added to the test suspension during the test and measured The reference measurement is used to compensate for changes in light levels from the luminescent bacteria The light levels change with time 12 10 1 Prepare a 9 dilution series D 2 values and higher To make a 9 dilution series according to ISO 11348 of D 2 sample values and higher do this procedure This procedure makes dilutions ranging from undiluted to a dilution ratio of 1 16 in Row A This corresponds to D values of 2 to 32 in the test Figure 6 as 0 5 mL of the sample dilution is added to 0 5 mL of the test suspension during the test in Row B and C Adding
57. in position A1 P 94 10 Put the tip of the pipette 11 Slowly dispense the 2 12 Do steps 10 and 11 in to the 2 NaCl solution NaCl solution into the test again to put 1 5 mL of 2 and slowly pull in 1 5 mL tube in position A8 NaCl solution in each test tube in positions A7 A6 A5 A4 and A3 13 Set the 1 0 5 0 mL 14 Put the tip of the pipette 15 Slowly dispense the pipette to 2 0 mL in to the sample and slowly sample in to the test tube in pull in 2 0 mL position AY 16 Put the tip of the pipette 17 Slowly dispense the 18 Set the 1 0 5 0 mL in to the sample and slowly sample in to the testtubein pipette to 1 5 mL pull in 2 0 mL position A2 95 19 Put the tip of the pipette 20 Slowly dispense the in to the sample and slowly sample in to the test tube in pull in 1 5 mL position A8 ae 3 J t l 22 Pull the solution in A9 in to the pipette 2 to 3 times to mix the sample dilution 5 6 7 8 9 10 23 Start making the sample dilution series in Row A Pull in 1 5 mL of solution from A9 and put it in to A7 using the pipette Pull in the solution in A7 in to the pipette 2 to 3 times to mix the sample dilution 96 21 Do steps 19 and 20 again to put 1 5 mL of the sample in to the test tube in position A10 Figure 10 on page 97 shows the contents of the test tubes in Row A after this step is completed 24 Pullin 1 5 mL of solution from A7 and put it in to A5 u
58. inescent Bacteria Test If the measurement range is set to the 0 2000 light units and the measuring value is marked with an e g 2010 or the lumiometer shows Detector Overload the measurement is above the set measurement range If this occurs dilute the bacterial stock suspension with Diluent and do the reading again To show or change the measurement range for the Luminescent Bacteria Test 1 Push ON green button to apply power to the instrument The instrument does built in tests that make sure that the electronics and software are operating correctly 2 When the built in tests are done push PROCEED The Main Menu is shown 3 Select LUMINESCENT BACTERIA TEST and push ENTER The LBT Main Menu is shown 4 Select Set Up and push ENTER The LBT Set Up Menu is shown 5 Select Set Measurement Range and push ENTER The current range is shown 6 Push YES to confirm 7 To change the range push CHANGE 8 Push STORE to save the change The Set up Menu is shown 17 3 3 Change the default settings 3 3 1 Set the LCD contrast The luminometer comes from the factory with the LCD contrast set correctly Do this procedure to increase the luminometer LCD contrast for low light conditions 1 Push ON for several seconds to apply power to the instrument The instrument does built in tests that make sure that the electronics and software are operating correctly 2 When the built in tests are done push PROCEED
59. information DANGER Indicates a potentially or imminently hazardous situation which if not avoided will result in death or serious injury WARNING Indicates a potentially or imminently hazardous situation which if not avoided could result in death or serious injury CAUTION Indicates a potentially hazardous situation that may result in minor or moderate injury Important Note Indicates a situation which if not avoided may cause damage to the instrument Information that requires special emphasis Note Information that supplements points in the main text 2 1 2 Precautionary labels Read all labels and tags attached to the instrument Personal injury or damage to the instrument could occur if not observed A symbol if noted on the instrument will be included with a danger or caution statement in the manual This symbol if noted on the instrument references the instruction manual for operation and or safety information Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after 12 August of 2005 In conformity with European local and national regulations EU Directive 2002 96 EC European electrical equipment users must now return old or end of life equipment to the Producer for disposal at no charge to the user Note For return for recycling please contact the equipment producer or supplier for instructions on how to return end of life equipment producer supplied e
60. inhibition that is not caused by the sample e Let the sample sediment for 1 hour or e Centrifuge the sample e g 10 minutes at 5 000 g 2 Check the pH level Adjust the sample to pH 6 to 8 using HCI or NaOH Use a strength of HCI or NaOH that does not change the volume of the sample by more than 5 in total 3 Add one spoon of solid NaCl LCX058 and dissolve it in 7 mL of sample The concentration of salt in the test should not exceed 35 g L Note Do not add NaCl to the sample if the salt concentration of the sample is more than 20 g L guide value conductivity of 35 mS cm Note The salt content of the sample should not exceed 50 g L This corresponds to a conductivity of about 70 mS cm without taking other conductive compounds in to account 56 Solid NaCl is used to change the sample osmolarity to a value that is correct for the marine bacterium used in the test 4 Ifthe sample has a high toxicity carry out a preliminary dilution of the sample with 2 NaCl solution Select a preliminary dilution from the levels 1 2 1 4 1 8 1 16 etc to make sure of a continuous dilution series using the dilution procedure of the manufacturer 11 11 Prepare the test tubes At the end of this procedure the test tubes contain the percent sample dilutions shown in Figure 3 ae 20 50 Figure 3 Screening dilution series 1 Test suspension 3 Sample OOOO WoO IBooo WOOO 1 2 3 4 l 1 Put four test tube
61. ith the standard solutions refer to the ISO standard procedure The necessary information about standard substances test concentrations and 50 sources of supply is contained in the quality certificate that comes with every box of luminescent bacteria reagent The standard stock solutions should be prepared with 2 NaCl solution The pH of the sample should not be adjusted Prepare the standard solution such that 0 5 mL of standard solution and 0 5 mL of bacteria solution gives the above mentioned final test concentration Check in duplicate whether those standard tests give 20 80 inhibition after 30 minutes of exposure time at 15 C 11 4 Reagent storage and preservation The freeze dried reagent can be kept at 18 C until the expiration date shown on the package Tubes that contain thawed but not reactivated freeze dried luminescent bacteria can be frozen again and kept on stock The reagent can be transported or shipped up to 7 days at no more than 25 C 11 5 Prepare the reagent Prepare the Luminescent Bacteria Toxicity Test reagent in the field using the procedure in this section The amount of light made by the luminescent bacteria is affected by the temperature at which the reagent is reconstituted The luminescent bacteria and reconstitution solution must be mixed as cold as possible at refrigerator temperatures 3 to 8 C If the temperature is higher the amount of initial light made by the bacteria will be lower
62. lectrical accessories and all auxiliary items for proper disposal This symbol when noted on a product enclosure or barrier indicates that a risk of electrical shock and or electrocution exists This symbol if noted on the product indicates the need for protective eye wear 2 2 Product overview The Eclox Rapid Response Test Kit is used to do first line water testing The kit is a generic qualitative test that gives a broad indication of water quality To do a proper toxicity test identify a baseline using the product in the waters to be tested The kit can be used to e Compare and prioritize possible source waters that might be used in a purification process to make drinking water e Give information to an operator to help the operator identify the correct water treatment process for the quality of the source water available e Be a regular quality assurance test on the drinking water made or the water given for drinking and if applicable on the source water Other configurations are available including the Eclox toxicity only kit and the luminometer by itself 2 2 1 Test descriptions Eight tests are included in the Eclox Rapid Response Test Kit e Chemiluminescence Toxicity Test shows the toxicity of the water sample This test uses a plant enzyme which when mixed with other reagents creates light chemiluminescence Pollutants in the water sample prevent this reaction which reduces the amount of light
63. light changes the color of the strip 3 Add the contents of one Reagent 1 and one Reagent 2 powder pillow to the reaction vessel See Interferences on page 34 lons or substances that are interferences lon or Substance Concentration Sulfide gt 15 ppb 34 Table 2 lons or substances that are interferences lon or Substance Concentration Do not acid preserve samples If Acidity samples have been acid preserved adjust pH to between 5 and 6 before starting the test 1 See section 5 3 1 on page 35 for information on removing sulfide Table 3 lons or substances that do not interfere at levels tested Alkalinity 1000 ppm as CaCOz 100 ppr 5 3 1 Removing sulfide optional Only do this step if sulfide is in the sample at interfering levels a rotten egg smell can be smelled after adding Arsenic Reagent 1 Always clean your hands thoroughly after touching lead acetate 1 Soak a cotton ball 2472 01 with lead acetate 14580 42 with the dropping bottle a few drops Pinch the excess liquid out of the cotton so that the cotton is damp 2 Push the soaked cotton ball in to the small opening of the reaction vessel cap from the bottom Make sure that the cotton is firmly put in with a gap between the cotton and the top surface of the cap 3 Putthe test strip in as usual and do the test Note The lead acetate must not touch the test strip 35 Visit us at www hach com Section 6 Pesticide Nerve A
64. lution series D 2 values and higher cccceeeeeees 85 12 10 2 Prepare a 3 dilution series D 2 values and higher ccccceeeees 89 12 10 3 Prepare a 9 dilution series D 1 values and higher cceceeeees 93 12 10 4 Prepare a 3 dilutions series D 1 values and higher cccceeee 98 12 11 Measure the light intensity of the test SUSPENSION cccceesseeeeeeeeeeeeees 101 12 12 Measure the light intensity of the test suspension after the sample dilutions are added cece ccecceceeeeceeeeceeeeeeeeeseeeeaeeesseeeeseeeeaaees 106 12 13 Show or send previous results cccccceecccceeceeceeeeeceeseeseseecsseessueeesaneees 108 Section 13 MaintenanCe 22 a ees 111 13 1 General MalMleNnanG Cicero sos escesenc betenetes ences eedeeb aes enaes wheeeceads eaeecgenelee ibepetss 111 TSetelGleaming tne Kiliceroe sete ae at oe 111 13 1 2 Cleaning the luminometer cccccceeeeeeceeeeeeccceeeeecsceueeseeseaeeeesseaees 111 13 2 Decomamninatl o Mice cs oe GS coe ie hs oe hae a ee el ian Oe 111 13 3 Batley TEDIACOMO IW cerci a a Ea AES 112 13 3 1 Luminometer battery replacement ccccceeeecseeeeceeeeeeeeeeeeaeeeeeeaes 112 13 3 2 Pocket Pal battery replacement pH and TDS cccceeeeeeees 112 Section 14 Troubleshooting cccccccceeceececeeeeeeeeeeeeeesaaeeeeeeseeeeeessaeeeeesaeeeees 115 Section 15 Replacement parts and accessories
65. ly 11 The Luminescent Bacteria Toxicity LBT Test can be done using either the e Measurement Luminescence procedure used in the lab when a thorough assessment of the inhibitory effects of a sample is necessary Use the LBT measurement luminescence procedure if the test needs to be done according to ISO 11348 Screening Luminescence procedure used in the field or in an emergency situation when a rapid assessment of the inhibitory effects of a sample is necessary The LBT screening luminescence procedure is a simplified test procedure that uses the same reagents according to ISO 11348 but at ambient conditions e LIMIT measure procedure the same as the screening luminescence procedure However the LIMIT measure procedure lets the user set a LIMIT value on the luminometer The LIMIT value is used by the luminometer to include in the test results whether the percent inhibition is above or below the LIMIT value 2 2 2 Test setup There are three basic operations to be done when using the kit e Pre deployment checks complete before starting on a series of tests Refer to the Quick Start Guide 28878 88 on the lid of the case e Luminometer test test the operation of the luminometer before Chemiluminescence Toxicity Tests or Luminescent Bacteria Toxicity Tests are done e Luminometer calibration calibrate the luminometer each day before Chemiluminescence Toxicity Tests are done e Measure samples measure the samples wi
66. ly pushing in the operating button Put the tip in to the liquid and then remove from the liquid 6 Remove the tip from the pipet and put in the waste bag Put the pipet in the storage case 25 4 3 Calibrate the luminometer Calibrate the luminometer before doing the Chemiluminescence Toxicity Test and after preparing the reagents The luminometer needs to be calibrated with every new batch of chemiluminescence reagents To calibrate the luminometer 1 10 11 12 13 14 15 26 Push ON green button to apply power to the luminometer The luminometer does built in tests that make sure that the electronics and software are operating correctly When the built in tests are done push PROCEED The Main Menu is shown Select ECLOX and push ENTER The ECLOX Main Menu is shown Push ENTER to select Measure The Measure Menu is shown Select Measure Reference and push ENTER Open the luminometer lid and make sure a sample is not in the cell Then close the lid Push PROCEED The test status is shown The test may go a few minutes before it is done When all the cell tests are done push PROCEED Open the lid of the Cuvette and 1000 uL Pipette Tip Set Put one cuvette in the black cuvette holder Place a blue pipette tip on the blue pipette Completely push in the operating button on the pipette to the stop and put the pipette tip in deionized water about 1 cm below the surface Rel
67. ment CV is 7 in the middle of the measuring range of 10 90 inhibition In practice samples that shows results of 15 inhibition in the 80 sample concentration have no affect in the Luminescent Bacteria Toxicity Test If higher precision or lower CV is needed do the LBT measurement luminescence procedure under more controlled conditions in a lab using additional accessories like a LUMIStherm temperature controlled incubator LTV053 11 3 Reagent description The Luminescent Bacteria Toxicity Test reagent contains living luminescent bacteria that have been grown under optimal conditions harvested and lyophilized freeze dried The reagent is a freeze dried preparation of a specially selected strain of the marine bacterium Vibrio fischeri formerly known as Photobacterium phosphoreum NRRL number B 11177 A vial of reagent contains roughly one hundred million test organisms Refer to section Appendix A Luminescent bacteria risks on page 125 for bacteria risk information 11 3 1 Quality assurance test The standards specify that certain validity criteria must be met for the reagent Accordingly a test must be done for each batch of bacteria that is prepared in house or moved in The quality certificate delivered with each package of luminescent bacteria reagent by HACH LANGE GmbH guarantees compliance with the stipulated validity criteria To make sure that the test operates correctly on site the user does control measurements w
68. n for several seconds to apply power to the luminometer When the built in tests are done push PROCEED The Main Menu is shown l i 6 Push PROCEED to show the test status When the cell tests are done push PROCEED again 7 Put one cuvette fromthe 8 Puta blue pipette tip on Cuvettes and 1000 uL Pipet Tip Set in to the black cuvette holder Pipette Opi 10 Put the tip in the sample water 1 cm below the surface Slowly release the operating button to pull in the sample 13 Puta yellow pipet tip on the yellow pipet Use a new pipet tip for each reagent the blue pipet Opi 11 Put the tip in to the cuvette and dispense the liquid by gently pushing in the operating button 4 D uL Pipette 9 Push in the operating button on the pipet to the stop 12 Remove the tip from the pipet and put in the waste bag Put the pipet in the storage case 14 Do steps 9 to 12 to put 100 uL of CT Reagents 1 2 and 3 in to the cuvette 15 Open the luminometer lid Remove the cuvette from the cuvette holder Gently tap the cuvette twice to mix the solution Put the cuvette in the luminometer cell 29 16 Close the lid Push PROCEED The luminometer automatically starts measuring After four minutes the screen timer shows DONE 19 Remove the cuvette from the luminometer cell Put the solution in to the waste bottle Put the cuvette in to the waste bag
69. n eb mL Conc Menu ao ET 6 Measure test tube 1 Pepp s j2 fer foe for fam 7 Measure the sample tubes in the order 2 3 and then 4 table ope ete 64 11 13 Show or send previous results To show previous results on the luminometer do the procedure in this section for the type of procedure done To send previous results to a computer Note At this stage the results can not be sent to the LUMISsoft 4 1 Do the steps in Connect the luminometer to a computer on page 21 2 Start LUMISsoft 3 In LUMISsoft select Transfer Options Interface Protocol Connect 4 Do the procedure in this section for the type of procedure done To send previous results to a printer do the steps in Connect the luminometer to a printer on page 19 and then do the procedure in this section for the type of procedure done 11 13 1 Description of screening luminescence results Reference non toxic luminescent measurements are saved as R1 to Rx The counter starts with R1 every time the storage is erased from the luminometer Sample luminescent measurements are done after a reference luminescent measurement is done Sample luminescent measurements are saved as S1 to Sx The luminometer records reference and sample measurements and then calculates the percent inhibition value for each sample measurement refer to Figure 4 For example two different screening luminescence tests have been done One test with 3 samples or sample dilutions
70. n to the black test tube holder in the luminometer cell 13 Record the measured value on a sheet of paper if the measured value is shown but not saved to the luminometer saved to the computer or printed 16 Close the luminometer lid Push MEASURE 14 Open the lid of the luminometer Remove the test tube from the luminometer and put it back in the LUMIStherm A AAN A B C i i ve w vw 17 If testing D 2 values and higher put the pipette in test tube A1 and pull in 0 5 mL of sample dilution If testing D 1 values and higher put the pipette in test tube A1 and pull in 0 8 mL of sample dilution Put the pipette in to the test suspension and slowly dispense the sample in B1 Mix with the pipette 103 18 When the C1 measurement is done record the measuring value on a sheet of paper if the measuring value is shown but not saved to the luminometer saved to the computer or printed 19 Open the lid of the luminometer Remove the test tube from the luminometer and put it back in the LUMIStherm 21 Close the luminometer lid Push MEASURE 104 22 If testing D 2 values and higher put the pipette in test tube A1 and pull in 0 5 mL of sample dilution If testing D 1 values and higher put the pipette in test tube A1 and pull in 0 8 mL of sample dilution Put the pipette in to the test suspension and slowly dispense the sample in C1 Mix with the pipette 2
71. ndard 55 Interfering Interference levels and treatments substances A sodium chlorine NaCl concentrations of less than 15 g L or more than 50 g L or their osmolarity equivalents in a sample will cause osmosis related light inhibition The addition of solid NaCl to the sample 2 final concentration prevents osmosis related light inhibition of samples of low or unknown NaCl concentrations Sodium chloride This biological test is strongly temperature dependent ISO 11348 requires that the test is done under temperature controlled conditions at 15 C using a appropriate thermostat i e LUMIStherm LTV053 Temperature Causes high bias results due to physical absorption or scattering of light Turbidity and color Use color correction cuvettes accessories in a separate test according to ISO 11348 or dilute the samples i e 25 or 50 before testing in the screening measure to remove the interference 11 10 Prepare the sample To prepare the sample for testing 1 Ifthe sample is turbid either e Filter the sample with a modified polysulfone filter Before using other filter materials test the filter material with 2 NaCl first to make sure that the filter material can be used with the Luminescent Bacteria Toxicity Test Check the acceptable filters in the ISO method Note Do not use a cellulose nitrate or a cellulose acetate filter The use of cellulose nitrate or cellulose acetate filters can cause light
72. o a PC start LUMISsoft on the computer start the test on LUMISsoft and when Please select LSoft at the Luminometer is shown select Measure Luminescence and Send to PC and push ENTER The luminometer must be connected to a computer refer to Connect the luminometer to a computer on page 21 e To measure the luminescence and print the results on a printer select Measure Luminescence and Send to Printer and push ENTER The luminometer must be connected to a printer refer to Connect the luminometer to a printer on page 19 101 6 Open the luminometer lid and remove any sample that is in the cell Close the lid C Proceed OO 0O 7 Push PROCEED to show the test status When the cell tests are done push PROCEED again 8 If testing D 2 values and higher set the 0 2 1 0 mL pipette to 0 5 mL If testing D 1 values and higher set the 0 2 1 0 mL pipette to 0 8 mL 9 Open the luminometer lid 102 10 Put the test tube in position B1 in to the black test tube holder in the luminometer cell 11 Close the luminometer lid Push MEASURE we wenn Let Lt HRS MIN SEC Lt LILI HRS MIN SEC 12 While B1 is being measured set the timer to the correct contact time e g 15 or 30 minutes Start the timer When the measurement is complete approximately 15 seconds the luminometer shows the relative light intensity of the test tube 15 Put the test tube in position C1 i
73. of a product such as but not limited to lamps and tubing Contact Hach Company or your distributor to initiate warranty support Products may not be returned without authorization from Hach Company Limitations This warranty does not cover e Damage caused by acts of God natural disaster labor unrest acts of war declared or undeclared terrorism civil strife or acts of any governmental jurisdiction e Damage caused by misuse neglect accident or improper application or installation e Damage caused by any repair or attempted repair not authorized by Hach Company e Any product not used in accordance with the instructions furnished by Hach Company e Freight charges to return merchandise to Hach Company e Freight charges on expedited or express shipment of warranted parts or product e Travel fees associated with on site warranty repair This warranty contains the sole express warranty made by Hach Company in connection with its products All implied warranties including without limitation the warranties of merchantability and fitness for a particular purpose are expressly disclaimed Some states within the United States do not allow the disclaimer of implied warranties and if this is true in your state the above limitation may not apply to you This warranty gives you specific rights and you may also have other rights that vary from state to state This warranty constitutes the final complete and exclusive statement of
74. one andere ge luminometer If fault occurs again contact TCS 102 Can not store contrast settings meviGve power ane inen apply mui one luminometer If fault occurs again contact TCS Troubleshooting fm Page 115 Table 4 Luminometer troubleshooting 103 An internal error has occurred Remove power ang en app aaa lome luminometer If fault occurs again contact TCS 104 Can not read signal level Remove power and then apply power to the luminometer If fault occurs again contact TCS 105 Can not recall measurements Remove power andinen Apply ee loine luminometer If fault occurs again contact TCS io ICannotaoremeasuremenis ee POWSTIO IE luminometer If fault occurs again contact TCS 200 Initialization fault Remove power and then apply power to the luminometer If fault occurs again contact TCS Note TCS means contact Hach Technical Consulting Services 106 Can not store usage counter Remove power and then apply power to the luminometer If fault occurs again contact TCS Visit us at www hach com Section 15 Replacement parts and accessories Replacement parts Description Qty Item number Beaker 50 mL plastic each 1080 41 Battery Cover Screw Tool each 28880 00 Color comparator box each 1732 00 Color disc 0 100 and 0 500 color units each 2092 00 Color viewing tube glass 6 pkg 1730 06 Cuvette holder each 28879 00 Cuvette and 1000 uL pipette tip set 28 pkg 28874 00 Disc program each 28881 00 Long p
75. over 900 If the signal is 900 or over change the measurement range to the 0 2000 range Do not throw away the reagent set If the signal is below 300 the reagents are probably unusable due to temperature sensitivity and new ones are required Note If the reagent baseline is reading over 1000 or the luminometer shows Detector Overload change the measurement range to the 0O 2000 range and continue There is no need to throw away the chemiluminescent reagents 29 If the signal is below 300 again add another 100 uL of CT Reagent 3 to the cuvette and do the calibration procedure again 27 4 4 Measure pollutants in the water sample Start with fresh reference everyday and with each new reagent set If the measured light units for the reagents are over 900 change the measurement range to the O 2000 range and continue refer to section 3 2 3 Set the measurement range on page 16 1 Fill the beaker with 50 mL of sample water 0 4 Select ECLOX and push ENTER Select Measure and push ENTER Select Measure Sample and push ENTER 28 MTT p 2 If more than 0 4 mg L chlorine is present neutralize the sample by adding two drops of pre conditioner reagent to the sample beaker Note Two drops of pre conditioner reagent can neutralize up to 15 mg L of chlorine 5 Open the luminometer lid and make sure that a sample is not in the cell Close the lid L VO 3 Push ON green butto
76. own ett a Ai 4 Immediately put the cap 5 Remove the test strip on the reaction vessel and immediately compare Swirl to mix Do not shake the developed color to the or turn over Do not let chart on the test strip the sample touch the test bottle Use the row for 50 strip pad mL sample 0 500 ppb Let the reaction occur for 20 minutes Swirl two times Note For the best results Auanath ti iod read the strip outdoors in a GING E PEACUON PETIOR shady place Direct sunlight changes the color of the strip 33 5 2 EZ Arsenic 0 4000 ppb 0 35 75 175 1500 4000 1 Lift the flap on the black cap and put a test strip in to the groove The reactive pad should be against and over the small opening Hold in place by pushing the flap down ett ae gt SE 4 Immediately put the cap on the reaction vessel Swirl to mix Do not shake or turn over Do not let the sample touch the test strip pad Let the reaction occur for 20 minutes Swirl two times during the reaction period 5 3 Interferences Table 2 2 Use the small square sample vial to put 9 6 mL of the sample in to the reaction vessel The vial filled to the top is 9 6 mL 5 Remove the test strip and immediately compare the developed color to the chart on the test strip bottle Use the row for 9 6 mL sample 0 4000 ppb Note For the best results read the strip outdoors in a shady place Direct sun
77. pense the pipette to 2 0 mL in to the sample and slowly sample in to the test tube in pull in 2 0 mL position AY i 12 Set the 1 0 5 0 mL 13 Put the tip of the pipette 14 Slowly dispense the pipette to 1 5 mL in to the sample and slowly sample in to the test tube in pull in 1 5 mL position A8 14 5 6 7 8 9 10 15 Do steps 13 and 14 16 Pull the solution in A9 17 Start making the again to put 1 5 mL of the in to the pipette 2 to 3 times sample dilution series in sample in to the testtube in to mix the sample dilution Row A position A10 Pull in 1 5 mL of solution Figure 7 on page 89 from A9 and put it in to A7 shows the contents of the using the pipette he ree apnoea Pull the solution in A7 in to l the pipette 2 to 3 times to mix the sample dilution 87 3 4 5 6 7 8 18 Pull in 1 5 mL of solution from A7 and put it in to A5 using the pipette Pull the solution in Ad in to the pipette 2 to 3 times to mix the sample dilution 21 Pull in 1 5 mL of solution from A8 and put it in to A6 using the pipette Pull the solution in A6 in to the pipette 2 to 3 times to mix the sample dilution 88 123 4 5 6 19 Pull in 1 5 mL of solution from A5 and put it in to A3 using the pipette Pull the solution in A3 in to the pipette 2 to 3 times to mix the sample dilution 22 Pull in 1 5 mL of solution from A6 and put it in to A4 using the pipette Pull the solution in A4 in to the pip
78. pull in bacteria reagent bottle and reagent bottle to mix 1 0 mL slowly dispense the solution in to the reagent aS LAI Is HRS MIN SEC 10 Cool the reagent for 5 minutes in the cool box 53 11 7 Prepare the test suspension Prepare the test suspension stock suspension and Diluent mixture by doing the procedure in this section The Diluent is made according to IS011348 3 and makes sure that the test is not negatively affected by the presence of potassium K and magnesia Mg2 ions in the sample The Diluent is a specially made non toxic 2 sodium chloride NaCl solution that contains potassium and magnesia ions The marine bacterium in the reagent requires the osmotic protection that is given by the 2 NaCl in the Diluent The potassium and magnesium in the Diluent stabilize the light made over time This stabilization helps keep high negative inhibitions from getting with samples that contain potassium and magnesium ions TT t w Ow J Do not make Diluent or use substitutes my 1 Remove the Diluent 2 Put 14 0 mL of Diluentat 3 Remove the stock from the cool box refrigerator temperature in suspension rehydrated the reaction vessel using reagent from the cool box Remove the cap fromthe the pipette Diluent bottle Remove the rubber stopper from the reagent bottle g 1 00 4 Set the 1 0 5 0 mL 5 Put 1 mL of stock 6 Put the cap on the pipette to 1 0 mL suspension at refrigerator reaction
79. r tool or small flat bladed screwdriver until the reading is good for the pH of the buffer 7 0 or 4 0 10 0 pH Section 10 Pocket Pal TDS Tester 10 1 Use and care of the tester 1 Push ON OFF onetime 2 Remove the protective 3 Put the bottom of the to apply power to the cap from the bottom of the tester 2 5 to 8 9 cm 1 0 to tester tester 3 5 in in to the sample ii KY i ey 4 Gently mix the sample 5 Clean the bottom of the for several seconds with tester with water Put the the tester When the digital cap on the tester display is stable read the TDS value Note Keep or make the performance better by periodically cleaning the stainless steel electrode with isopropyl alcohol Note Readings may not become stable for up to 2 minutes especially if the temperature is far from ambient 47 10 2 Calibration 1 Make the TDS of a known calibration standard using the tester An 85 47 mg L standard is included in the kit 48 Calibration Trimmer a Trimmer Tool 2 If necessary change the calibration trimmer using the supplied trimmer tool or small flat bladed screwdriver until the reading is good for the concentration of the known calibration standard Section 11 Luminescent Bacteria Toxicity Test Screening and LIMIT measure The Luminescent Bacteria Toxicity LBT Test screening and LIMIT measure procedures use the luminometer Before doing either procedure e Read se
80. re the light intensity of the test suspension luminescent bacteria according to ISO 11348 using this procedure The Luminescent Bacteria Toxicity Test is a biological test method and the result is therefore strongly temperature dependent ISO 11348 states that the test must be done under temperature controlled conditions at 15 C using a thermostat LUMIStherm LTV053 In some instances if reconstitution is done at the optimum temperature and the test is carried out at 20 C the initial light made by the bacteria can be more than 1000 Eclox light units This causes the error Detector Overload If an error occurs change the amplification settings from 0 1000 to 0 2000 light units and do the readings again refer to Set the measurement range on page 16 To measure the light intensity of the test suspension 1 Push ON green button for several seconds to apply power to the luminometer When the built in tests are done push PROCEED The Main Menu is shown 2 Select Luminescent Bacteria Test and push ENTER 3 Select Measure and push ENTER 4 Select Measure Luminescence and push ENTER 5 Select one option that is shown e To measure luminescence and save the results on the luminometer select Measure Luminescence and Save and push ENTER e To measure luminescence and manually record the measuring values on paper select Measure Luminescence without saving and push ENTER e To measure the luminescence and send the results t
81. risk information The standards stipulate that certain validity criteria must be complied with for the reagent Accordingly a test is done for each batch of bacteria that is prepared in house or bought in The quality certificate delivered with each package of luminescent bacteria reagent by HACH LANGE GmbH guarantees compliance with the stipulated validity criteria To make sure that the test operates correctly on site do control measurements with the standard solutions refer to the ISO standard procedure The necessary information about standard substances test concentrations and sources of supply is contained in the quality certificate that comes with every box of luminescent bacteria reagent 73 12 5 Reagent storage and preservation The freeze dried reagent can be kept at 18 C until the expiration date shown on the package Tubes that contain thawed but not reactivated freeze dried luminescent bacteria can be frozen again and kept on stock The reagent can be transported or shipped up to 7 days at no more than 25 C 74 12 6 Prepare the reagent Prepare the Luminescent Bacteria Toxicity Test reagent not more than 4 hours before testing according to ISO 11348 as done in this section The amount of light made by the luminescent bacteria is affected by the temperature at which the reagent is reconstituted The luminescent bacteria and reconstitution solution must be mixed as cold as possible at refrigerator temperatures 3
82. rs 33 5 2 EZ Arsenic 0 4000 ppb 0 35 75 175 1500 4000 ec ceeeeee eens 34 SIMO TIG RCI CCS yeni ea r a edle ede hiatal sta a d 34 5 3 1 Removing sulfide Optional ccccccssseeeeceeeeeecceeeeeeseeseeeessueeeeesseaees 35 Section 6 Pesticide Nerve Agent TeSt cccccccceeesseeeeeeseeeeeseeeeeeeeseeeenens 37 6 1 Pesticide Nerve Agent procedure ccccccccseeeeeeeeeeeeeeeseeeeeeeeseeeeeessaeeeessaaaeees 37 Table of Contents EcloxTOC fm Page 3 Table of Contents Section 7 Free and Total Chlorine Test cccccccccseseeeeeseeeeeeeseeeeeeeeeaeeeees 39 7 1 Measuring hints and general information cccccseeeeceeeseeeeeeeseeeeeeeeeeeeeeeseaaees 39 7 2 Free chlorine procedure 0 3 5 MO L ccccceccccssececeeecesceeceeseeeeesseeesseeessaaaees 39 7 3 Total chlorine procedure 0 3 5 Mg L ccccceccceceeeecceeeeeceeeceeseeeeeseeeeeseeeessneeees 41 Section 8 Color TOSE o5ilecdier se hele le ee Steet eee ees 43 O21 Color TeSt lOW WAS 5 seh rsd oh iste S a a 43 Oz Colon Test NIN Vane cen Ne ie te ce a See Se 44 section 9 Pocket Pal pH Tester 24A i2 eld hte ha Ra enue 45 9 1 Use and care of the tester soca csehcsst poise wd aire ne aidnensebaneien dana nndeadedeuaehdea 45 92 Z POIMU Cale ON e aretha a en a al ake 46 section 10 Pocket Pal TDS Testelor aa 47 10 1 Use and care of the tester ccccccseccecceesseeeceeesececceeeeeeseeseeeessaeeseesseeseeessas
83. s and higher Prepare the test suspension for D2 values and higher if the sample is expected to be toxic t mi 1 Remove the Diluent 2 Put 50 parts Diluent 3 Remove the stock from the cool box solution D at refrigerator suspension rehydrated temperature in to the reagent from the cool box Remove the cap fromthe reaction vessel using a Diluent bottle pipette Remove the rubber stopper from the reagent bottle O TH a O i es c OOum O B 4 Put 1 part stock 5 Put the cap on the 6 Put one half of the new suspension S at reaction vessel and shake empty test tubes in Row B refrigerator temperature in to mix thoroughly and one half of the test to a clean reaction vessel tubes in Row C of the using a pipette LUMIStherm For example Note The LUMIStherm 0 2mLS 10mLD should be operating at 15 C 77 Blk u 7 Setthe 0 2 1 mL pipette 8 Put the end of the 9 Put the tip of the pipette to 0 5 mL pipette in to a clean pipette in to the reaction vessel tip and slowly pull in 0 5 mL of the test suspension 10 Slowly dispense the 11 Do step 9 and 10 again 12 Cool the filled test test suspension in to the until each test tube in Row tubes in the LUMIStherm at test tube in position B1 B and Row C contains 15 C for 15 minutes 0 5 mL of test suspension ia Ne 13 Remove the pipette tips from the pipettes and put in the waste bag Put the pipettes in the storage case 78 12
84. s in the 2 Set the 0 2 1 0 mL 3 Put the end of the pipette test tube stand pipette to 0 2 mL in to a clean pipette tip 57 u 1 2 3 4 4 Put the tip of the pipette 5 Slowly dispense the test 6 Do steps 4 and 5 again in to the test suspension suspension in to the test until all four test tubes and slowly pull in 0 2 mL tube in position 1 contain 0 2 mL of test suspension 0 80 1 2 3 4 7 Set the 0 2 1 0 mL 8 Put the tip of the pipette 9 Slowly dispense the 2 pipette to 0 8 mL in to the 2 NaCl and NaCl solution in to the test slowly pull in 0 8 mL tube in position 1 P ije a X 10 Set the 0 2 1 0 mL 11 Put the tip of the pipette 12 Slowly dispense the 2 pipette to 0 6 mL in to the 2 NaCl and NaCl solution in to the test slowly pull in 0 6 mL tube in position 2 58 A 13 Set the 0 2 1 0 mL 14 Put the tip of the pipette pipette to 0 3 mL in to the 2 NaCl and slowly pull in 0 3 mL 17 Set the timer clock for 15 minutes contact time 16 Set the 0 2 1 0 mL pipette to 0 2 mL 20 Set the 0 2 1 0 mL pipette to 0 5 mL 19 Slowly dispense the sample in to the test tube in position 2 Start the timer Note No sample is put in to the test tube in position 1 Test tube 1 is the non toxic reference 1 2 3 4 15 Slowly dispense the 2 NaCl solution in to the test tube in position 3 Note No 2 NaCl is put in the test tube in position 4 So 1
85. s should not be considered avirulent simply because they are vaccine strains Biosafety Level 1 represents a basic level of containment that relies on standard microbiological practices with no special primary or secondary barriers recommended other than a sink for hand cleaning A 3 Disposal The luminescent bacteria are harmless and can be put down the laboratory drain Make sure to dispose of toxic samples correctly Contact the local regulatory agency for correct disposal information 1From Biosafety in Microbiological and Biomedical Laboratories BMBL 4th Edition HHS Publication number CDC 93 8395 U S Department of Health and Human Services Centres for Disease Control and Prevention and National Institutes of Health U S Government Printing Office Washington DC 1999 126
86. scent Bacteria Toxicity Test is a biological test method and the result is therefore strongly temperature dependent ISO 11348 states that the test must be done under temperature controlled conditions at 15 C using a thermostat LUMIStherm LTV053 If the Luminescent Bacteria Toxicity Test is done at ambient temperature record the temperature The results of tests done at different temperatures can not be compared directly To measure the light intensity of the test suspension after the sample dilutions are added HRS MIN SEC 1 Wait until the contact 2 Open the luminometer 3 Put the test tube in time is completed lid position B1 in to the black test tube holder in the luminometer cell 106 5 Record the measured value on a sheet of paper if the measured value is shown but not saved to the luminometer saved to the computer or printed 4 Close the luminometer lid Push MEASURE When the measurement is complete approximately 15 seconds the luminometer shows the relative light intensity of the test tube um 8 Put the solution in the test tubes in to the waste bottle 7 Do steps 3 to 6 again until all the test tubes in Row B and Row C have been measured Measure the test tubes in the same order that the light output of the test suspension was measured e g B1 C1 B2 C2 etc 6 Open the lid of the luminometer Remove the test tube from the luminometer and put it back in to the
87. shown To measure luminescence and save the results on the luminometer select LIMIT Measure and Save and push ENTER To measure luminescence and manually record the measuring values on paper select LIMIT Measure without Saving and push ENTER To measure the luminescence and send the results to a PC start LUMISsoft on the computer start the test on LUMISsoft and when Please select LSoft at the 61 Luminometer is shown select LIMIT Measure and Send to PC and push ENTER The luminometer must be connected to a computer refer to Connect the luminometer to a computer on page 21 To measure the luminescence and print the results on a printer select LIMIT Measure and Send to Printer and push ENTER The luminometer must be connected to a printer refer to Connect the luminometer to a printer on page 19 N O E HRS MIN SEC 6 Open the luminometer 7 Push PROCEED to 8 Wait until the timer clock lid and make sure a sample show the test status When completes 15 minutes is not in the cell Close the the cell tests are done lid push PROCEED again 9 Open the luminometer 10 Put the test tube in 11 Close the luminometer lid position 1 non toxic blank lid Push MEASURE 62 in to the black test tube holder in the luminometer cell When the measurement is complete approximately 15 seconds the luminometer shows the relative light units measured 12 Open the lid of the luminometer Remove the test t
88. sing the pipette Pull in the solution in Ad in to the pipette 2 to 3 times to mix the sample dilution Ae ee 3 J JE 26 Pull in 1 5 mL of solution from A8 and put it in to A6 using the pipette 7 8 9 10 25 Pull the solution in A8 in to the pipette 2 to 3 times to mix the sample dilution Pull in the solution in A6 in to the pipette 2 to 3 times to mix the sample dilution mM MN mee mie HRS MIN SEC 28 Keep the dilution series at 15 C for at least 5 minutes to correct the temperature A1 A2 A3 A4 A5 A6 A7 27 Pull in 1 5 mL of solution from A6 and put it in to A4 using the pipette Pull in the solution in A4 in to the pipette 2 to 3 times to mix the sample dilution A10 A8 A9 Figure 10 Before the dilution series is started 9 dilutions D 1 and higher 1 Sample 2 2 NaCl solution 97 12 10 4 Prepare a 3 dilutions series D 1 values and higher To make a 3 dilution series according to ISO 11348 of D 1 sample values and higher do this procedure This procedure makes dilutions ranging from undiluted to a dilution ratio of 1 1 5 in Row A This corresponds to D values of 2 to 3 Figure 11 in the test as 0 5 mL of the sample dilution is added to 0 5 mL of the test suspension during the test in Row B and C Adding test suspension to the sample dilutions increases the sample dilutions in row A by a factor of two as final test concentration Figure 11 shows the contents of
89. t on page 112 Chemiluminescence line develops in a bell shaped curve The chemiluminescence reagents are weak and need to be replaced Do the reference measurement again Negative percent inhibition Salt could be in the sample or the reagents may be faulty Erase all measurements refer to section 3 2 2 Erase the results saved on the luminometer on page 15 Memory failure Contact Technical Consulting 01 System RAM test failed Services TCS 02 System EPROM test failed Memory failure Contact TCS 03 LCD display test failed ed operation If fault occurs again contact 04 Non volatile RAM test failed Memory failure Contact TCS 5 All saved measurements will be erased Continue 0 Storage measurements corrupt j i operation If fault occurs again contact TCS LCD contrast and measurement range will be Configuration settings corrupt reset Continue operation If fault error occurs again contact TCS Usage counter corrupt Usage counter will be reset to zero Continue j operation If fault occurs again contact TCS Database is full No new measurements can be saved A D input failure Failed measurement Contact TCS Reference LED failure Failed measurement Contact TCS Reference LED reading fault Failed measurement Contact TCS 07 11 100 Can not clear measurements Remove power ane inen apply nail tone luminometer If fault occurs again contact TCS Remove power and then apply power to the a wan Heb st
90. th the tests 2 3 Unpack the instrument Remove the Eclox Rapid Response Test Kit from the shipping carton and check it for any visible damage If any items are missing or damaged contact the manufacturer or a sales representative immediately Refer to the packaging guide supplied with the kit for equipment descriptions Visit us at www hach com Section 3 Luminometer 3 1 Overview The Eclox luminometer is used with the Chemiluminescence Toxicity Test and the Luminescent Bacteria Toxicity Test to measure and record relative light units made by the reagents when exposed to samples The Eclox luminometer is made for use under extreme field conditions The Eclox luminometer components are rugged easy to use and reliable refer to Figure 1 Figure 1 Luminometer components 1 5 2 Battery compartment 6 Cell lid 3 Battery compartment screws 7 Function keys 4 Label 8 Display 13 Figure 2 Luminometer buttons umber Doseripton Punton Does the action on the display directly above the key 3 2 Prepare the luminometer for use Do the procedures in this section before each deployment to prepare the luminometer for use 3 2 1 Test the operation Do this procedure to make sure that the luminometer is operating correctly If the luminometer passes all the tests done in this procedure it is operating correctly To test the operation of the luminometer 1 Open the hinged cell lid of the luminometer and make sure
91. the sample for at F in to the luminescent the reagent bottle Swirlthe least 15 minutes in a bacteria reagent bottle reagent bottle to mix refrigerator Quickly dispense the solution in to the reagent 12 6 2 Prepare the test suspension Prepare enough test suspension stock suspension and Diluent mixture to do the test Each test tube used for the test is filled with 0 5 mL of test suspension To identify the number of test tubes used for a test e For D 2 values an higher add 1 to the number of sample dilution steps to be measured e g 1 blank 9 dilutions 10 Then multiply that number by 2 e For D 1 values and higher add 2 to the number of sample dilution steps to be measured 2 blanks 3 dilutions 5 Then multiply that number by 2 The Diluent is made according to IS011348 3 and makes sure that the test is not negatively affected by the presence of potassium K and magnesia Mg2 ions in the sample The Diluent is a specially made non toxic 2 sodium chloride NaCl solution that contains potassium and magnesia ions 76 The marine bacterium in the reagent requires the osmotic protection that is given by the 2 NaCl in the Diluent The potassium and magnesium in the Diluent stabilize the light made over time This stabilization helps keep high negative inhibitions from getting with samples that contain potassium and magnesium ions Do not make Diluent or use substitutes 12 6 2 1 Test suspension for D 2 value
92. the test tubes in Row A at the end of this procedure D1 D2 g A1 A2 A3 A5 Figure 11 Dilution series 3 dilutions D 1 values and higher 1 Sample 2 2 NaCl solution g 1 00 123 4 5 1 Put 5 empty test tubesin 2 Set the 1 0 5 0 mL 3 Put the end of the pipette to Row A of the pipette to 1 0 mL in to a clean pipette tip LUMIStherm 98 h 4 Put the tip of the pipette 5 Slowly dispense the 2 6 Set the 1 0 5 0 mL in to the 2 NaCl solution NaCl solution in to the test pipette to 1 5 mL and slowly pull in 1 0 mL tube in position A4 h 7 Put the tip of the pipette 8 Slowly dispense the 2 9 Do steps 7 and 8 again in to the 2 NaCl solution NaCl solution in to the test to put 1 5 mL of 2 NaCl and slowly pull in 1 5 mL tube in position A3 solution in to the test tube in position A1 10 Put the tip of the pipette 11 Slowly dispense the 12 Set the 1 0 5 0 mL in to the sample and slowly sample in to the test tube in pipette to 2 0 mL pull in 1 5 mL position A5 99 13 Put the tip of the pipette in to the sample and slowly pull in 2 0 mL HRS MIN 16 Keep the dilution series at 15 C for at least 5 minutes to correct the temperature 100 14 Slowly dispense the sample in to the test tube in position A4 15 Do steps 13 and 14 again to put 2 0 mL of sample in to the test tube in position A2 12 11 Measure the light intensity of the test suspension Measu
93. ube from the luminometer and put it back in the LUMIStherm Ty 15 Put the solution in the test tubes in to the waste bottle 13 Do steps 22 to 24 again to measure the three other test tubes in the correct order 2 3 and then 4 The Inhibit and rel units are shown on the screen Record the Inhibit and rel units values for each sample dilution on the Screening Luminescence Results Sheet N Ny 16 Put the test tubes in to the waste bag 14 Use the color chart on the Screening Luminescence Results Sheet to identify which sample dilutions are toxic red and which are non toxic green Note The more of your results in the red zone the stronger are the inhibitory affects of the sample the more critical is the sample 63 Luminescent Bacteria Toxicity Test Screening Luminescence Results Sample Date Time Operator Comments Procedure 1 Add 1 0 mL of reconstitution solution to the reagent Swirl to mix Wait 5 minutes 2 Add 1 0 mL of stock suspension to 10 mL of Diluent Shake to mix Wait 15 minutes c Ae 2 T os 3 Add one spoon of solid NaCl to 7 mL of sample 4 Fill four test tubes with the test suspension 2 NaCl and sample in the order shown in the table Start the 50 8 timer after adding the first sample sample volume Set the timer for 15 minutes epep E enea the luminometer Go to the Tube Test 2 e ee Screening Luminescence rei
94. vessel and shake temperature in to a clean to mix thoroughly reaction vessel using the pipette 54 Y mm wry if FILS J HRS MIN SEC ED onan NAi A 7 Wait 15 minutes 8 Remove the pipette tip from the pipette and put in the waste bag Put the pipette in the storage case 11 8 Sample collection storage and preservation The test can be used with samples of municipal and industrial waste water aqueous eluates from soil and waste aqueous solutions of pure chemicals and with surface well and water of other sources Collect samples in clean glass bottles Keep samples in the dark at 0 to 5 C for no longer than 2 days Freeze and store samples at 18 C for not longer than to 2 months Record preservation activities Before use defrost samples completely Homogenize the defrosted samples 11 9 Interferences Samples interferences can inhibit the light made by luminescent bacteria Interferin g Interference levels and treatments substances Changes the viability of the bacterial reagent Chlorine is toxic to the bacteria Chlorine To remove chlorine from a sample add one powder pillow of sodium thiosulfate Hach 1436369 dechlorination agent to 20 mL of sample and wait for 10 minutes High oe Causes light inhibition that is not caused by toxicity consumption pH related light inhibition may occur if the pH is below 6 0 or above 8 0 The pH of the sample must be within 7 0 2 pH units of the sta
95. x 353 0 1 4 50 93 37 info hach lange ie www hach lange ie HACH LANGE FRANCE S A S 33 Rue du Ballon F 93165 Noisy Le Grand T l 33 0 1 48 15 68 70 Fax 33 0 1 48 15 80 00 info hach lange fr www hach lange fr HACH LANGE APS Akandevej 21 DK 2700 Br nsh j Tel 45 36 77 29 11 Fax 45 36 77 49 11 info hach lange dk www hach lange dk Repair Service in the United States HACH Company Ames Service 100 Dayton Avenue Ames lowa 50010 Tel 800 227 4224 U S A only Fax 515 232 3835 HACH LANGE GMBH Willstatterstrake 11 D 40549 D sseldorf Tel 49 0 2 11 52 88 320 Fax 49 0 2 11 52 88 210 info hach lange de www hach lange de HACH LANGE GMBH H tteldorferstr 299 Top 6 A 1140 Wien Tel 43 0 1 9 12 16 92 Fax 43 0 1 9 12 16 92 99 info hach lange at www hach lange at HACH LANGE SA Motstraat 54 B 2800 Mechelen T l 32 0 15 42 35 00 Fax 32 0 15 41 61 20 info hach lange be www hach lange be HACH LANGE AB Vinthundsv gen 159A SE 128 62 Sk ndal Tel 46 0 8 7 98 05 00 Fax 46 0 8 7 98 05 30 info hach lange se www hach lange se Repair Service in Canada Hach Sales amp Service Canada Ltd 1313 Border Street Unit 34 Winnipeg Manitoba R3H 0X4 Tel 800 665 7635 Canada only Tel 204 632 5598 Fax 204 694 5134 canada hach com HACH LANGE LTD Pacific Way Salford GB Manchester M50 1DL Tel 44 0 161 872 14 87 Fax 44 0 161 848 73 24 info hach lang

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