LifeECO Thermal Cycler User Manual
Contents
1. M 15 4 3 3 Backlight adjustment E 16 Instruction Manual of LifeECO Thermal Cycler 4 3 4 Hot lid setting 16 LLL 16 436 Soren calibration 7 7 PIN NM 16 MEM rc 17 4 3 8 Tm Calculator 17 4 4 Distribution characteristics of gradient module nnne 17 CHAPTER V CONNECT TO COMPUTER 18 5 1 System configuration 18 Ace ESL LLL oT 18 Boconnect to 19 SJ BufisOllWal amp tert tuned eer een e catu ed udine 20 5 4 1 User 20 5 4 2 File MERI M 21 5 4 3 Equipment management 23 5 4 4 Other TMAMAGEIMENNA MEMENTO 25 sm V MEME T 26 CHAPTER VI FIRMWARE UPGRADING 28 6 1 Connect to PC _ _______ ___ ____ 28 6 2 SOT WAS 28 VI FAILURE ANALYSIS AND TROUBLESHOOTING 29 T 1 Failure Analysis and Troubleshooting _ _ A AEE O 29 Appendix 1 Wiring Diagram O9UO 0O 92. 00 10 VS 00 10 SIGE VU 3 Alter password Select the file that needs to alter password and click alter password Input old and new passwords in the pop up dialog and click to save Do you want to change password of Computer gt test gt 117 If the selected file is not protected by password then Enter old password m it only needs to input new password Enter new password awe The password can contain only letters and numbers Change file password 4 Rename Select the file that needs to be renamed in the Change file name equipment list and click rename j cannot A Do you want to change file name of Computer gt test gt 11 renamed when the file is opened Enter new file The file can contain only letters and numbers 5 Delete file oelect the file that needs to be deleted in the equipment list and click delete E If the file is being edited then it cannot be deleted If the file is protected by password then it cannot be deleted unless inputting correct password 22 23 Instruction Manual of LifeECO Thermal Cycler 6 Copy Select the file that needs to be copied and click copy Lh or right click copy file in the menu to copy the file If the file is being edited then it cannot be copied 7 Paste Select the equipment option in the equipment list and click
3. TECHNOLOGY LifeECO Thermal Cycler INSTRUCTION MANUAL Instruction Manual of LifeECO Thermal Cycler Caution The Bioer Co reserves the right to modify this manual at any time without notice The manual contains copyright protected and patented material Without prior written consent from Hangzhou Bioer Technology Co Ltd any part of the manual shall not be duplicated reproduced or translated into any other language Before initial use of this instrument please carefully read this manual Product Patent No ZL 20092019972313 Product Standard No YZB Z 0189 2010 The product is for scientific research only Document No BYQ90780000000ESM Document Version 2013 Version 1 2 Instruction Manual of LifeECO Thermal Cycler IMPORTANT NOTES 1 General Practice Caution Important information is contained in any item and should be carefully read Failure to observe the instruction would result in damage or abnormal functions to instrument Warning The warning message requires extremely careful operation of a certain step Failure to observe the instruction would result in serious personal injury 2 Safety During operation maintenance and repairment of this instrument the following basic safety notes shall be observed In case of failure to follow these measures or the warnings or notes indicated herein the basic protection provided by the instrument its safety criteria of design and manufact
4. 2 After setting temperature control mode increased amount of fluid and temperature of hot lid click to run PCR program 98 0 98 0 98 0 00 30 0 Hotid heat 338 15 3 Lid temp 315 00 20 01 00 Block temp stop Suspend next section Program running interface Instruction Manual of LifeECO Thermal Cycler can click main menu to return to the main menu or carry out other operations such as creating new file If you want to return to the program running interface again you can click the running interface at the top of the screen on the Ver 1 00 Block typle 96 0 2 NN 2 R NewFile Main menu in program operation 4 2 2 Run saved PCR program V Recent 3 123456 3 7 F 4 Share 123 V TTTYREEYUT Y V 1 3 2 3 8 3 10YRJRWY7432 4 4 G 4 V 5 4 V 4m 666666666666 4 123 F 654321 F 1 page 1 of2 page 1 of 1 T Select User New User Delete User Rename New File Main Option interface for users Option interface for program file Under the main menu clicks run to enter Click corresponding user name to enter option interface option interface for users as the above for programs as the above figure In the option interface figure In the option interface for users the for programs the user can create new file edit file delete user
5. 123 w e ts 123 oe tee Ei Save Run Winzard User TEST d SampleTypo 96 0 2 sj e 18 Control Mode Z4 Tube Block Sample Volume J o Contro Ok x Cancel Delete File Edit File New File Parameter setting interface Instruction Manual of LifeECO Thermal Cycler The mode Block only takes into account of the temperature control in rise and fall of the module Since the rise and fall of the reagent lags behind the module to certain extent the actual temperature change process of the reagent is different from the program we set The actual time during which the reagent reaches the setting temperature will be far less than the setting time see the relationship between module temperature and reagent temperature in the right figure 2 Hh gt 2 mode takes into account of the backward process during which the rise and fall of the reagent lags behind the module After reaching the setting temperature there will be an overshooting process so that the reagent can reach the setting temperature at a quicker speed In the setting time that similar to that of Block mode the actual time during which the reagent achieves the setting temperature evidently prolongs see the relationship between module temperature and reagent temperature in the right figure
6. 7 Export user Click export to select the folder saved by the user Click OK to export user If there is same user name then it will prompt whether to cover in export If the program file under the user is being edited then the user cannot be exported 5 4 2 File management Include creating renaming deleting copying pasting importing and exporting user etc mo Save Change Rename Delete Copy I Export password File operation 1 Create Select the user that needs to create file and click create 4 Input file name and password and click OK Create file Do you want to create new file in test of Computer Enter new file name The file name can contain only letters and numbers Enter password The password can contain only letters and numbers 3 Cancel Instruction Manual of LifeECO Thermal Cycler 2 Edit and save Select the file that needs to be edited and click edit or right click edit file Click in the menu bar after finishing edition LI Gd ow x s Copy Export Creste Save paration File operati 1 213 504 z 04r 0 0 Ext 0 0 ws 04 I 00 00 Est Tim 0040 Ext T 00 00 Li wei 0 1 0 Boc ime 41 wa 0 rr or 4 T 4 v1
7. Ver 1 00 Block typle 96X0 2 B NewFile System _04 0 oystem main menu Setting interface of PCR program Instruction Manual of LifeECO Thermal Cycler 4 1 1 Add or delete program segment You can add or delete program segments by clicking segment delete 1 at the bottom of the screen on the setting interface of PCR program 98 0 98 0 98 0 98 0 980 1 00 30 00 05 gt 72 4 00 30 00 30 00 05 72 0 72 0 00 20 01 00 00 20 1 0 0005 99 2 1 00 05 04 0 lt gt Delete 4 1 2 Segment temperature and constant temperature time You can set temperature of program segment and constant temperature time by clicking temperature or time on the screen on the setting interface of PCR program 98 0 98 0 98 0 00 30 72 0 72 0 00 20 01 00 00 30 00 05 Temp and time set Temp 98 0 0 80 30 30 mmiss Edit program segment Instruction Manual of LifeECO Thermal Cycler 4 1 3 Cycle function On the setting interface of PCR program first click the program segment that needs to add cycle function Then click jump to enter the setting interface of cycle function as shown in the right figure Junp Setting Repeat Times 20 03 98 0 00 05 05 72 0 00 20 The jump function takes effe
8. paste 77 or right click paste copied file in the menu to paste the program file under the user and the user name if the copied user has already existed then it will prompt whether to cover in pasting If the program file under the existed user name is being used then it cannot be pasted You can use mouse to drag and paste 8 Import file Click import nd and select the folder of the user in the pop up window Click OK to import file If there is same name then it will prompt whether to cover n Wal eme in importing SORS tolls If the file with the same name is in use then it cannot E imported MR E Pictures newpcr110627 Videos File folder testear iM Computer EAR File amp 05 lt 4 00 KB File name test ear v EarFile ear 9 Export file Browse For Folder Click export and select the folder saved by the user in Please select export file s target path the pop up window Click OK to export file If there is same file name then it will prompt whether to cover in exporting If the file is being edited then it cannot be exported ag Libraries gt zhs p e Computer P lt Network b 28 Control Panel Recycle Bin L GeneTouchSetup 20110809 newpcrl 10627 5 4 3 Equipment management Include connection operation connection and disconnection and instrument manag
9. SEMA V ec Cap ENIT 4 8 28148 02 5 38 PAM N asna asna az LMA 7 2 3 wacce p m 3614 3804 J VEAOSZOV nitus 5 10095 65 PR 4 2 019939 3513 Yet TE EH 3 AG TIE SH Bit VEH YAL GERIN E sh BER ELINT wn T LLIN 92 013 FH RE E HAZIZLG3V Flv 00002220 905 60 e _ 0 rv Xm wu 00 ef w00 17 SL3NI 5 iis 00001 1092050A8 99 00002LL082080A8 ee vod QNO ft 4 ab gt 23 99 98 93 93 99 v7 ON 0 AH 2 8 3 5 86 85 2881 LLNO 1 9n AH 2 9 9f 31 995 1 0000111082090 8 89d 31 Instruction Manual of LifeECO Thermal Cycler MEMORANDUM 32 BloER TECHNOLOGY Add 1192 Bin An Rd Hi tech Binjiang District Hangzhou Tel 86 571 87774575 RM 87774303 Fax 56 5 E mail overseas bioer com cn PU 310053
10. 1 Normal working conditions Ambient temperature 10 30 Relative humidity lt 7096 Power supply 100 240V 50 60Hz Caution Before using the instrument please make sure the working condition meets the above requirements Especially the power line must be reliably earthed 2 2 Transportation and storage conditions Ambient temperature 20 55 C Relative humidity lt 80 2 3 Basic parameters Product name Product No Model Sample size LCD display Temperature range Max heating rate Max cooling rate Temperature uniformity Temperature control precision Temperature fluctuation Gradient range Gradient range of temp differential Temperature range of hot lid File saving amount Energy consumption Operation mode Dimensions Fuse PC interface Net weight LifeECO BYQ6078 TC 96 G H b C 96 holes x0 2ml 96 holes full apron plates half apron plates no apron general and 12x8 test tube bar Color touch screen 5 7 260 000 colors 4 99 C gt 2 JS Eq 204 C lt 0 3 test after 30s 20200 30 99 TO 300 110 gt 250 600W Continuous running mode 335 x 260 270mm 250 5 20 USB 8kg Parameters are tested in standard environment Instruction Manual of LifeECO Thermal Cycler 2 4 Software functions a Edit the file including file number temperature and time of each segment cycle times setting file
11. 31 Instruction Manual of LifeECO Thermal Cycler CHAPTER INTRODUCTION The contents in this chapter include introduction of PCR technology examples of PCR application introduction of Thermal Cycler and features of LifeECO Thermal Cycler 1 1 Introduction of PCR technology Polymerase chain reaction PCR is a nucleic acid amplification that simulates natural DNA replication in vitro It takes 2 DNA chains to be amplified as template uses a pair of artificially synthesized oligonucleotide primer as mediator and amplifies certain DNA fragment quickly and specifically by thermostable DNA polymerase Ever since PCR technology was invented by Mullis in 1985 and thermostable DNA polymerase was discovered by Erlich in 1988 this simple fast specific and sensitive technology has been widely applied to life science areas at astonishing speed Remarkable achievements have been witnessed in cytology virology oncology genetic disease forensic medicine animal plant immunology and etc It is a milestone in contemporary molecular biology development 1 2 Example of PCR technology application scope Research Clinical diagnosis Immunology Human genome project Forensic medicine Oncology Histology population biology Paleontology Botany zoology Gene clone dna sequencing mutation analysis gene recombination and fusion protein regulating dna sequence identification transposition insertion site mapping gene
12. a PCR instrument with multiple functions and several applications Below are its features Use advanced thermoelectric refrigeration technology from ITI of US and thermoelectric refrigeration elements made under quality management of Japan which are steady and reliable in quality Temperature can be changed as fast as 4 second The speed to increase or decrease temperature can be set as per need of experiment Several modes for temperature control better compatibility of reagent O Large LCD screen user friendly operation interface simple and easy operation O Power off protection available programs can continue after power recovery Height and pressure of hot lid can be set for different reaction tubes which effectively prevent the reagent from evaporation and pollution Connect to ordinary computer by USB standard interface which is easy for software upgrading 1 4 Use of LifeECO Thermal Cycler Integrating a number of advanced technologies LifeEco Thermal Cycler meets the demand of different PCR experiments and ranks top in similar products LifeECO Thermal Cycler is attached with 1 2 Strip of 8 tube 3 Strip of 12 tube 4 Full half apron plates 0 2 ml standard centrifugal tube Instruction Manual of LifeECO Thermal Cycler CHAPTER II FEATURES This chapter introduces the usage transportation storage conditions of the instrument as well as its basic parameters performance and functions 2
13. can create new user delete user and file and run selected file etc rename 14 Instruction Manual of LifeECO Thermal Cycler 4 3 Set system parameters Click system setting in the main menu to enter main menu of system parameter setting Click touch screen to enter corresponding system setting interface 4 3 1 Date amp Time Click date amp time on the main menu of system setting to enter date amp time setting interface Click OK to save after setting 4 3 2 Language Click language 84 on the main menu of system setting to enter language setting interface The instrument has integrated two languages namely Chinese and English Click OK to save after setting pt 1 jJ i Date amp Time Language Backlight Heat cover Beep Touch screen USB Tm calculator Re Main menu of system setting Date amp Time Date 2051 05 25 yy mm dd Time 12 29 34 Hh mm ss BBBBBBEU T Date amp time setting interface Language English Chinese Japanese SY Ok x Cancel Language setting interface Instruction Manual of LifeECO Thermal Cycler 4 3 3 Backlight adjustment Click backlight adjustment on the main menu of system setting to enter backlight adjustment setting interface Press the navigation bar to move right and left to adjust backlight brightness of the instrument Click OK to save a
14. d Power wire Generally this instrument uses accompanied power wire The damaged power wire shall be replaced by one power wire of the same type and the same specification It can t be repaired The power wire shall neither be compressed nor be laid in gathered personnel location e Insertion and withdrawal of power wire At insertion and withdrawal of power wire the plug shall be correctly held with hand At insertion the plug shall be completely and tightly inserted into the socket and at withdrawal heavily pulling the wire shall not be allowed f Placement of instrument This instrument shall be fixed in a low RH and low dust place apart from water source e g water pool and water pipeline and the room shall be well ventilated and free from corosive gas and interterence of strong magnetic field The instrument shall not be placed in a wet or dusty location The openings on this instrument are for ventilation circulation and in order to avoid over heat of the instrument body they shall not be blocked or covered When a single set or several sets of instrument are used the interval between its surrounding ventilation opening and its nearest object shall not be below 50cm or 100cm Also don t use the instrument on loose or soft surface or the air inlet at instrument bottom might be blocked Excessive temperature would impair the test performance or result in instrument failure This instrument shall not be used in location subjected to direct
15. system information contains beep alarm RN 3 key sound completion sound temperature keep sound and Complete ON hot lid setting the lowest temperature of hot lid and module Soak OFF retention temperature Clicks save after finishing setting Module Lowesthotlid temperature 400 Hotlid will clase when temperature lowes this The lowest hot lid temperature range 30 C 110 An Module keep temperature 20 HEC Module retention temperature range AC 30 C Madule keep this temperature when hotlid temperature is rising up The system information cannot be set when the instrument is TT disconnected Lowest hatlid temperature 30 0 When there is only single module in the connected instrument EE SR PES UE WES Wi the display area of module B cannot be altered keep this temperature when hotlid temperature is rising up 5 4 4 Other management 1 lemperature gradient Click temperature gradient 1 in the menu bar and a gradient dialog box as follows will pop up am 53 mAg cH Temperature gradient range temperature 30 1057 Gradient temperature 1 30 u Lt 40 ea Ng RIS J 5 1 10 T Instruction Manual of LifeECO Thermal Cycler 2 System help Click help then the help file will appear 3 Software edition Click about the system dialog box will pop up and the software edition can be referred
16. to 5 4 5 Run 1 Run file Open the file and click then a run file dialog box like the right figure will pop up Select instrument Do you want to run file Please select instrument or module and input parameters Tube Control Instrument viime method Hotlid temp Verti run method ____ 5 155 module test tube type amount of increased fluid temperature control mode hot lid temperature and running method limited to Verit Module Click OK to run program Selectall Unselect all After running the file the running status on the v Online Offline right of the main menu shows like the right figure test GeneTouch 48D Module temp 27 3 e B Module temp 29 9 C Details of instrument operating status are as follows Ban flle 50 _ mui slop d _ _ Tube wali Tube lype odin tame ED E temp 00 onome run lime h rritis Current segment r miainimg Erie Denis ee ITUR ac AXE s um Ext Ixt Tamp DA 4 Exi Tumi 09 Bel Time 200 05 Ext Tima 000 Grad 76 din rad amp 27 Instruction Manual o
17. view revision and deletion automatic file connection gradient temperature setting the maximum paragraph step and cycle times for editable file are 10 100 and 99 respectively The scope for gradient temperature setting is 1 C 30 b Save the file Revise the file adjust the rate for temperature rise fall refine temperature and time for cycling automatically and display and calibrate the time d Display the temperature time cycle times setting and real time value during the file running including sound prompt total rest time estimation for file running failure protection and alarming e Stop the file running including pause the file running stop the file running recover automatically after power off Caution above mentioned software function is merely for reference The software function would be modified without notice Instruction Manual of LifeECO Thermal Cycler CHAPTER PREPARATION This chapter mainly introduces structure of the instrument and preparation before startup When using the instrument for the first time read the contents in this chapter carefully before startup 3 1 Structure diagram Hot lid Touch screen Pen buckle USB interface Fixed location Touch pen Installation method Caution The fixed location can be adjusted according to your need Take the touch pen out of the accessory and strip the When fixing the location must be determined Then s
18. click USB amp the main menu of system setting The equipment will automatically search for U Input file disk The user can export file to the U disk Output file after successful searching USB search interface EL Caution 1 USB read write user number is 100 every user s file number is 250 2 USB read write fixup list USB root list descended pcrex folder use xx ebr file of the folder 3 Every user is one son folder of pcrex folder 4 Forbid to insert and pull out USB flash memory very fast To protect apparatus and USB flash memory if insert and pull out USB flash memory the apparatus will close USB function until the next boot strap 5 The operation method for double module is the same the link of USB flash memory is only for two modules under the original interface 4 3 8 Tm calculator Click Tm calculator on the main Im menu of system setting to enter tm calculator interface There are two blank Annealed Pia box in the interface through the keyboard to calculate tm value Tm calculatoe interface input annealed product click Calculate 4 4 Distribution characteristics of gradient module The gradient module adopts 2 temperature control units The temperature gradient distributes like a curve See as the following figure set temperature at 40 C and temperature gradient at 20 Each hole temperature gradient of the module do not distribute evenly Temperature diff
19. ct only when it jumps from the later program segment to the former one See as the above figure 05 seg03 In setting you need to enter the program setting status of 05 then click jump to set cycle function 2880 98 0 98 0 00 30 00 20 01 00 00 05 Goto Finished jump setting Instruction Manual of LifeECO Thermal Cycler 4 1 4 Extended parameters You can enter extended temperature li extended time speed and gradient and other operations by clicking extended parameters Ext temp lt D Exttemp Ext time Rate Grade c M EE Be Extended temperature temperature increment per cycle Back Cance il Ext time Exttemp Ext time Rate Grade All view OE Extended time time increment per cycle ui Lr Rate Exttemp Ext time Rate Grade All view we ENIM opeed heating and cooling speed 2 5 4 5 6 iLe 7 9 Grade Exttemp Ext time Rate Grade All view Temp Grade Gradient temperature gradient refers to the i 2 720 tempearture difference among the most left column and the most right column of the 072 0 module temperature of the most left 0720 0720 0720 072 0 i column is set in the temperature o72 9 072 0 072 0 7 7 I Ok X x Canc
20. ddress is not allowed to be used in different instruments Search instrument Eam 197 158 U 1 192 168 O 255 Serial port Set instrument name Select to add on the instrument Communi cation Address 820110422 _ SerialPort COM3 820110423 _ SerialPort COMS Select all Unselect all Automatic connection OK Add instrument Instrument Communication method Port Instrument address 24 25 Instruction Manual of LifeECO Thermal Cycler 5 Edit instrument Select equipment name sub item in the equipment list and click Edit instrument edit instrument amp amp An equipment edition dialog box like the Instrument name right figure will pop up input instrument name and instrument adaress Instrument address When connecting instrument the instrument address LOM cannot be edited communication method Seri gl 6 Remove instrument Select equipment name sub item in the equipment list and click remove instrument The instrument cannot be removed when it is connected 7 Show all instruments Click all instruments and a list of all instruments will appear to show all instruments information 8 Instrument system information Local gt Setting Select system setting a setting dialog box like the right figure SUE Beep alarm when run finish will appear Instrument
21. e Instruction Manual of LifeECO Thermal Cycler Display all browse the current setting of extended parameters and gt means to move the cursor left or right means to alter negative and positive data Clr means to clear data 4 1 5 Save Run All view Exttemp Ext time Ext temp Ext time Rate Grade OK Goto Delete Rate Grade Allview 2 0 0 0 1 00 00m00s 4 0 5 12 After setting PCR program click save run to enter save interface as shown in the right figure The user input user name and file name in the interface and also can adopt password assess mechanism for the data Finally clicks save User Name File Name Password Save interface of PCR program CEEE Instruction Manual of LifeECO Thermal Cycler The user can directly select the existing file under the user name by clicking icon as shown in the right figure 4 2 Running PCR program 4 2 1 Run new PCR program 1 After saving the new PCR program click run to enter parameter setting as shown below user can alter temperature control mode increased amount of fluid and temperature of hot lid including opening closing hot lid function mm eral FileName 9525212 Password 1 10 Password 4ovRIRWY7432 11
22. e Comprise user management file management and equipment management etc Menu bar Instrument contents File display and edition area Display area of running status otatus bar pri e 5 4 1 User management Include creating renaming deleting copying pasting importing and exporting user etc 1 Create user oelect equipment name sub item in equipment list and click create or right click create user The user name shall not be empty The max length of user name is 12 bytes The user name shall only contain numbers and letters under half width mode A same user name cannot be used for more than one time in a same instrument 2 Rename oelect the user needing to be renamed in the equipment list and click or right click alter user name Click OK to save after inputting user name It cannot be renamed when the file under the user name is opened 3 Delete user Select the user needing to be deleted in the equipment list and click delete or right click delete user to delete amp m Rename Delete Copy User operation Create user Do you want to create new user in Computer Enter new user name The user name can contain only letters and numbers Change user name Do you want to change user name of Computer s test 2 Enter new user The user name can contain only letters a
23. ement searching for instrument adding instrument editing instrument removing instrument and showing all instruments an x LM 3e Ao search Add 7 sempe All Instrument instrument instrument Connect operation Instrument manage Instruction Manual of LifeECO Thermal Cycler 1 Connect Select equipment name sub item in the equipment list and click connect or right click connect equipment in the menu It will show f in connection show disconnection 2 Disconnect Select equipment name sub item the equipment list and click disconnect amp If the file or system parameters in the equipment are being edited then the dialog box will pop up prompt for save 3 Search for instrument Click search for instrument then a dialog box like the right figure will appear Select added equipment and input equipment name Click OK to save If the instrument needs to be connected please select automatically connect 4 Add instrument Click add instrument a and a dialog box like the right figure will appear Input instrument name and address Click OK to add instrument The instrument name shall not be empty and the max bytes shall be 12 Only numbers and letters are allowed in the instrument name The same instrument name is not allowed The instrument address shall not be empty and the same a
24. erence of each hole in the middle of the module will be larger than that of holes on the two sides of the module Therefore when operating gradient module temperature of each hole shall be subject to the temperature displayed on the instrument Gradient graphs Temperature C SROGONROSO 1 2 3 5 6 T 8 9 10 11 12 Colum n left to right Instruction Manual of LifeECO Thermal Cycler CHAPTER V CONNECT TO COMPUTER 5 1 System configuration Operating system Windows XP Windows Vista Windows 7 System environment Operating environment Net Framework2 0 Other software PDF reader CPU Pentium R 4 Minimum configuration Memory 1GB Hard disc 10GB 5 2 Install software Click ThermalCyclerSetup exe in CD ROM to install online program 30 instruments can be controlled simultaneously by connecting to computer program jB Setup ThermalCyder Welcome to the ThermalCyder Setup Wizard This will install ThermalCycler version 1 0 on your computer It is recommended that you close all other applications before continuing 1 Click and run ThermalCycler Setup exe to select installation language and click OK Click Next to continue or Cancel to exit Setup ie Setup ThermalCycler Select Destination Location Where should ThermalCycler be installed Setup will install ThermalCycler into the Following Folder 2 Select installation location
25. f LifeECO Thermal Cycler 2 Browse running history Right click system history and a history table as follows will pop up local gt Hixtery m Username File name Bagin tima End time Siate 5 002 2011 04 11 3 29 04 2011 04 11 13 31 20 Usersto user nten 184323300 _ user 20110411 18 42 38 Run Instruction Manual of LifeECO Thermal Cycler CHAPTER VI FIRMWARE UPGRADE 6 1 Upgrade preparation LifeECO firmware upgrades with the new USB disk upgrade mode First of all prepare a USB disk with FAT32 file format Please do not use NTFS format Then copy the firmware file BYQ6078 cpu bin to the pcrex directory of USB flash disk root directory If without pcrex directory please create one manually After that rename BYQ6078 cpu bin file Firmware bin 6 2 Firmware upgrade Please insert the prepared USB disk and press the touch screen while opening the instrument and entering into the upgrade mode After click the UPGRADE button the program will automatically read the firmware file TCE Firmware bin and start the upgrade process When the window shows Upgrade Finish it means the upgrade 15 successful Now please restart the instrument 28 29 Instruction Manual of LifeECO Thermal Cycler CHAPTER VII FAILURE ANALYSIS AND TROUBLESHOOTING This chapter mainly introduces possible failures of this instrument and reason anal
26. fter setting Backlight adjustment setting interface 4 3 4 Hot lid setting Click hot lid setting on the main menu of system setting to enter hot lid setting interface The first is when the module temperature below a certain value need to shut down hotlid prevent hotlid temperature on the influence of the temperature of module The second is when cover heating modules need to keep a constant temperature untill hotlid temperature up to hotlid set temperature Click OK to save after setting hot lid setting interface 4 3 5 Tone Click tone on the main menu of system setting to enter tone setting Tone setting interface 4 3 6 Screen calibration Click screen calibration on the main menu of system setting to enter screen calibration setting screen In the setting process you can finish screen calibration function by clicking upper left corner of the screen and figure center at the lower right center in turn with touch pen as per the prompt Screen calibration interface Back light 79 Close cover while module temp below Keep module temp When cover heating Back e e lt Cancel End x Cancel Instruction Manual of LifeECO Thermal Cycler 4 3 7 USB Usb disk Insert USB and
27. he PCR THERMAL CYCLER file its running as well as setting of instrument system parameters etc Caution sound occurs or the display is abnormal failure alarming and tone occurs during the process of the self test of the instrument after the instrument startup please cut off the power supply immediately and contact the supplier Caution If the sample quantity in your experiment is less than the number of taper holes of the module please try to distribute the sample test tubes evenly when placing tubes so as to ensure that the hot lid can be stably pressed on top of the test tube when running the file Meanwhile make the load of the module even to guarantee that the temperature changes of each test tube are uniform Caution Turn the knob in a counterclockwise way as required in knob figure press tightly in clockwise direction and release in counterclockwise direction before closing the lid until hearing twice clang sound Then turn the knob in a clockwise way before startup Turn another round after hearing twice clang sound Twice clang sound will be heard no matter turning the knob in a counterclockwise or clockwise way Unlock device knob can be released when pressing the unlock device and turning two rounds in a counterclockwise way simultaneously 4 1 Creating PCR program Click new file 3 in the main menu enter setting interface of PCR program 98 0 98 0 00 30 00 05 72 0 72 0 62 0 00 05 00 20 000 N
28. he module neither heats nor cools manufacturer Cooling chips are all damaged In system parameters setting interface Set the hot lid status at a certain the hot lid status is set as OFF temperature value Temperature control in file edition 6 Hot lid cannot heat interface is set as so that the Set the temperature time as number form hot lid automatically closes Connectors get loose Hs Contact the supplier or the manufacturer Temperature sensor of the hot lid is damaged 5 Chip is in poor connection screen shows abnormal characters Chip is broken Contact the supplier or the manufacturer Instruction Manual of LifeECO Thermal Cycler S N Failure Reason Analysis Troubleshooting Have not set hot lid temperature Please refer to 4 3 in Chapter IV of the the hot lid is set as OFF Instruction on Setting Hot Lid Temperature Adjust hole location of the reaction tube 8 Reagent in reaction tube evaporates The reaction tube is unevenly placed try to place in a symmetrical way Please tighten the reaction tube The reaction tube is not tightened before placing it into the instrument S N 2 Err code 0x 000 Ox Left sensor Right sensor Reserves Reserves Reserves Reserves Hotlid sensor of single module sensor Heat sink sensor sensor of double module of double module 0 OK 1 Opencircuit alarm 2 Short ci
29. hermal Cycler 2 1 4 Use of LifeECO Thermal Cycler 2 CHAPTER FEATURES 3 2 1 Normal working conditions 3 2 2 Transportation and storage conditions eene 3 2 3 Basic RR 3 2 4 Software functions 4 CHAPTER PREPARATION 5 3 1 Structure LLL 5 3 2 Examination before 6 3 3 Startup 6 CHAPTER IV OPERATION GUIDELINE A 7 4 1 Creating PCR program EMT M 7 4 1 1 Add or delete program Segment 8 41 2 Seoment emperaus and 8 41 3 js eue 9 4 1 4 Extended parameters 10 NNI RU arae S S S SS stances NO cece ee 11 4 2 Running PCR program MEN 12 4 2 1 Run new PCR 91616 A A 4 A 12 4 2 2 Run saved 14 4 3 Set system parameters 15 EAE o S T AT 15 Language
30. nd numbers The program file under the user name will be simultaneously deleted when deleting the user 21 Instruction Manual of LifeECO Thermal Cycler 4 Copy Select the user needing to be copied in the equipment list and click copy right click copy user in the menu to copy the user and the program file under the user name It cannot be copied when the program file under the user is being edited 5 Paste Select the user sub item under equipment option in the equipment list or equipment option in the equipment list and click paste or right click paste in the menu to paste the user and the program file under the user name If the copied user has already existed it will prompt whether to cover when pasting If the program file under the existing user name is in use then pasting cannot be carried out Pasting may be finished by dragging mouse 6 Import user Browse For Folder Click import to select the folder saved by the user in the pop up dialog box Click OK to import user Please select import user If the user selected has no program file then user cannot be B Desktop gt Libraries If there is same user then it will prompt whether to cover in 4 Computer importing gt fy 05 b cw DATAPARTI D If the program file under the same user name is in use the user B gt efi DVD Drive E cannot be imported ig Network
31. of the To continue click Next IF you would like to select a different Folder click Browse software and click next ber am At least 2 7 MB of free disk space is required Setup ThermalCycler Select Start Menu Folder Where should Setup place the program s shortcuts Setup will create the program s shortcuts in the Following Start Menu Folder 3 Click next continue click Next IF you would like to select a different Folder click Browse rhermalCyder Browse Instruction Manual of LifeECO Thermal Cycler ie Setup ThermalCycler Ready to Install Setup is now ready to begin installing ThermalCycler on your computer TE n Click Install to continue with the installation or click Back if you want to review or Glick install change settings Destination location C BioerlThermalCycler Start Menu Folder ThermalCycler Setup ThermalCycler Completing the ThermalCycler Setup Wizard Setup has finished installing ThermalCycler on your computer The application may be launched by selecting the installed 5 Finish installation Click finish oe Click Finish to exit Setup 5 3 Connect to PC Connect the equipment with the computer through USB cable sag 1 USB cable Ie Computer Instrument 19 Instruction Manual of LifeECO Thermal Cycler 5 4 Run softwar
32. rcuit alarm Heat sink over temperature alarm The extwrnal environment is too row will cause radiator alarm not instrument anomaiy Caution 1 The user is not allowed to open the housing of the THERMAL CYCLER for inspection by himself during the warranty period If any failures that require opening the housing for inspection occur the supplier or manufacturer should be contacted timely 2 Since different brands and all instruments have their own temperature control characteristics including heating and cooling rate stability and volatility and due to the uncertainty of biological experiment and external vulnerability successful PCR program effect in one instrument may not apply to another Therefore when you change the instrument please adjust PCR running program so as to achieve the ideal state 30 Instruction Manual of LifeECO Thermal Cycler and is subject to change without further notice For reference only iring W Appendix 1 000002101 Z08049 989d 000012108 0 0 8 NH ex Hf 589 07100006 86 LOA 0100000065510 9 WZ ccr sn 2909 08 10 0 001 IBS dor vzr 8779318 9zr SEI HIE gr HS uni umo 813 11 243 11 BAT 91 3 7 HE AS LA Sek TINT EAN TG al TEES 114 23
33. se skin would be burnt 4 Maintenance of instrument In order to ensure full contact and good heat conduction between the test tube and the conical holes the conical hole on the module shall be regularly cleaned with clean soft cloth soaked with a little absolute alcohol The stained surface of this instrument if any shall be cleaned with soft cloth soaked with cleaning paste Warning During cleaning the instrument its power supply shall be cut off The instrument surface shall not be cleaned with corrosive cleaning agent 5 After sales service the warranty content and scope are shown in the warranty sheet Caution After unpacking immediately check the goods against the packing list In case of damaged or missed goods please immediately contact the supplier After qualification of acceptance properly fill the product acceptance sheet and send or fax the copied sheet to the supplier for filing and maintenance After unpacking the packing box and packing materials shall be well kept for future repairment For the damage of instrument occurring during transporting to the repair department due to improper package Hangzhou Bioer Technology Co Ltd shall bear no liability 4 Instruction Manual of LifeECO Thermal Cycler CONTENTS CHAPTER LINTRODUCTION 1 1 1 Introduction of PCR 1 1 2 Example of PCR technology application SCOPE 1 1 3 Features of LifeECO T
34. sunshine The instrument shall be kept away from hot gas stove and all other heat source If the instrument is to be set aside for a long time the power plug shall be withdrawn and the instrument covered with soft cloth or plastic film to aviod entrance from dust Instruction Manual of LifeECO Thermal Cycler g Cautions during operation During test cares shall be taken to prevent liquid from dropping onto the instrument Caution any of the following cases immediately cut off the power supply withdraw the power plug from the power socket contact the supplier or ask qualified repairman for treatment Liquid drops into inside of the instrument The instrument is rained or watered The instrument works abnormally especially generates abnormal sound or odor The instrument is dropped or its casing is damaged The function of instrument is obviously changed 3 Instrument label a Label Hangzhou Technology Co Ltd Model TC 96 G H b C c us ae Power 100 240V 600W 50 60 2 or Fuse AC250V 8A 5x20mm EpL cR SN BYQ6078 XXX Date Address Hangzhou China Made In China b Warning Sign Warning AD The instrument is adhered with mark HOT SURFACE During operation of instrument or a period immediately after completion of running of a program 2 any portion of body shall not be contacted with metallic portion module or 17 j IW HOT SURFACE hot lid nearby the said mark otherwi
35. tic modification detection synthetic gene construction clone construction carrier expression certain gene s incision enzyme diversity detection and etc Bacteria spirochete mycoplasma chlamydia mycobacterium rickettsia diphtheria bacillus pathogenic e Coli dysentery bacillus aeromonas hydrophilic and c Difficile and etc Virus htlv hiv hbv hcv hpvs cmv hsv measles virus rotavirus parvovirus b19 and etc Parasite malaria and etc Human genetic disease lesh nyhan syndrome mediterranean anemia hemophilia bmd dmd cystic fibrosis and etc HLA typing T cell receptor or antibody diversity qualification autoimmune disease gene mapping lymphokine quantification and etc DNA mark generated from scatter repetitive sequence genetic map construction detect DNA polymorphism sperm drawing physical map construction sequencing expression map and etc Crime scene specimen analysis hla dqa analysis and etc Pancreas cancer rectal cancer lung cancer thyroid cancer melanin cancer hematological malignant tumor and etc Genetic cluster research evolution research animal protection research ecology environmental science experimental genetics and etc Archeology and museum specimen analysis and etc Animal inflectional disease diagnosis plant pathogen detection and etc Instruction Manual of LifeECO Thermal Cycler 1 3 Features of LifeECO Thermal Cycler LifeECO Thermal Cycler is
36. trip the pen 3M rubber release layer at the back of buckle off from the back rubber release layer and fix it on the after determining the fix location Then paste it on the Instrument fixed location as shown Instruction Manual of LifeECO Thermal Cycler Module __ d Ventilation hole USB interface Power switch with fuse Power socket Caution The fuse specification must be 250V 5 20 Use qualified product to replace the fuse Contact fuse supplier or manufacturer when necessary 3 2 Examination before startup Please confirm the following contents before plugging in for powering on 1 Whether the power supply conforms to the required voltage see power supply requirements in Chapter II of the file 2 Ensure the plug of power cord has been reliably plugged in the socket 3 power cord is reliably grounded 3 3 Startup Turn on the power switch turn the switch at The Thermal Cycler will produce one toot sound It indicates that it is powered on At that time the screen is still black Please wait for about 10 20 seconds The main menu will then appear Now you can edit browse alter and delete PCR Thermal Cycler file as well as other operations Instruction Manual of LifeECO Thermal Cycler CHAPTER IV OPERATION GUIDELINE This chapter provides detailed introductions of the methods for the edition browsing alteration and deletion of t
37. ure and its predicted use range would be impaired Hangzhou Bioer Technology Co Ltd shall be irresponsible for the consequence resulted from user s failure to observe the following requirements Caution This instrument conforms to Class and Protection Class IP20 of GB4793 1 Standard and it is for indoor use This product has been tested to the requirements of CAN CSA C22 2 No 61010 1 second edition including Amendment 1 or a later version of the same standard incorporating the same level of testing requirements a Instrument earth In order to avoid electrical shock accident the input power wire of instrument shall be reliably earthed This instrument uses 3 core earth plug and this plug is provided with a third earth leg is merely used for earth type power socket and is a safety unit If the plug is impossible to insert into the socket the socket shall be correctly fixed by electrician otherwise the earth plug would lose safe protection function b Keeping apart from the live circuit The operator shall not disassemble instrument without permission Only qualified professionals are allowed to replace elements or adjust the machine inside Replacement of elements under energizing state shall be prohibited c Use of power supply Before turning on AC power make the power voltage consistent with the instrument required one 1096 deviation is allowed Make the rated load for power socket not below instrument s maximum load
38. ysis as well as troubleshooting 7 1 Failure Analysis and Troubleshooting S N Failure Reason Analysis Troubleshooting Power supply is not connected Check the power supply and connect 1 Alter the power supply switch is tumed on Fuse is burnt Replace fuse 250V 5x20 the display screen is not on and the toot HE sound is not heard either Switch is damaged switch Others Contact the supplier or the manufacturer Module sensor is damaged or in oor contact During file running process there is prompt 2 of Err code 0x 0000 Please Contact Heat sink sensor is damaged or in pp poor contact manufacturer the Manufacturer Hot lid sensor is damaged in poor contact The ventilation hole is blocked Clear obstructions at the vent 3 Obvious changes in module heating rate pil 1 Case 4 2 or inaccurate temperature control ignten the screws of wire terminals they get loose Cooling chip is damaged Contact the supplier or the manufacturer Please properly adjust the ambient is so high that they exceed application M conditions of the instrument d Temperature cooling rate of the module 4 obviously slows down or it cannot slow Cooling chip is damaged down under room temperature Contact the supplier or the manufacturer Fan is damaged or out of work The temperature sensor is damaged Contact the supplier or the T
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