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ImageMaster VDS-CL - GE Healthcare Life Sciences

1. 47 6 1 1 Loading a roll of film 48 6 1 2 Film select setting LL 50 6 1 3 Tonesetting 0 cee eren 50 6 2 Removing a roll of film 50 6 3 Clearing a film jam iii 51 6 4 Cleaning the Printer 52 6 4 1 Thermal head 52 6 4 2 Rubber roller ee 53 6 5 Printer pre sets 54 6 5 1 Restoring default printer parameters 57 6 5 2 UV transillumination procedure 58 6 5 3 White light imaging procedure 58 7 Troubleshooting 8 System maintenance 8 1 Care and cleaning 22 61 8 2 Replacing parts eee 61 8 21 UVIAMP3 esie nied b Ru uer n Eerburiw go 61 8 2 2 White light lamps llle 62 8 2 3 Replacingfuses e p E ee 63 A System Specifications B System Accessories Index Introduction EN 1 Introduction The ImageMaster VDS CL is a multifunction bio imaging station designed for quick and accurate photo documentation of electrophoretic and Chemiluminescent results The system includes a312 nm ultraviolet UV transilluminator sample tray for documentation of ethidium bromide stained agarose and polyacrylamide gels as well as excitation of fluorophorescent samples a Chemiluminescent tray for documentation of chemiluminescence samples a white light sample tray and va
2. This manual is designed as an introduction to the layout and functioning of the ImageMaster VDS CL system More detailed instructions on the following functions can be directly accessed via the online Help Menu Menus Dialogue Boxes Dialogue Bars Toolbar Status Bar im 18 1131 81AA e p14 System overview 3 7 Other system functions 3 71 Lights The user can operate upper or lower white lights only when the white light tray is inserted and the door is closed Upper and lower UV lights can only be operated when the UV drawer is in and the door is closed 3 7 2 Cutting option The UV drawer can be used to cut the sample on it To perform the cutting operation the UV drawer is inserted and pulled towards the user until the right panel in the status bar reads Cut amp Guide The Cut Light icon on the screen is then depressed triggering a 360 nm light from the lower lamp field This function is performed with the door open but the user MUST use visual protection 3 7 3 Positioning of samples When the drawer is inserted but the door still open the user can use the Guide Light icon on the screen to produce a laser spot The laser is directed to the center of the drawer and facilitates proper positioning of the blot 3 7 4 Camera The user can control all of the important camera parameters including focus iris zoom shutter or exposure time and gain 3 7 5 Save options The system allows the user to
3. resolution Close the door and focus the camera by pressing the AF button in the Optics Dialogue bar From this point you can select the right preset that matches your application from the Presets drop down menu or continue through step 12 Choose the appropriate filter for your application the CL application uses No Filter Set the Gain value in the Analog Dialogue bar to the appropriate value or 0 Unfreeze the image using the Freeze toolbar icon Choose the right illumination for your application for CL No illumination is used From this point you set the optimal conditions depending on which application you choose p13 ED System overview CL Applications 1 Set the Iris value to 1 2 using the Iris combo box in the Optics Dialogue bar 2 Set the Gain value to 180 and press Set to set it 3 Set the Exposure Tlme through the Analog Dialogue bar to match the application notes 4 If the resulting image is unsatisfactory either try Auto Y Gain Contrast or Brightness from the Digital Dialogue bar or change Exposure Time and Gain parameters to obtain a new image 3 6 On line Help Menu FL Applications 1 If there is too much light increase the Iris value and then if necessary increase decrease the Exposure Time through the Analog Dialogue bar 2 If there is not enough light increase the Exposure Time through the Analog Dialogue bar and then if necessary increase the Gain value
4. 20x 18 cm Filter Coomassie Gels 100 50 White 0 00 080 No 0 4 Lower 0 250 3 10x 8 cm Filter Silver stained gels 100 50 White 0 00 080 No 0 4 Lower 0 250 out 20x 18 cm Filter Silver stained gels 100 50 White 0 00 120 No 0 4 Lower 0 250 3 10 x 8 cm Filter 4 7 Help menu The Help menu contains the following commands Help Topics About IM VDS CL po EE Dam sielmlaj aj Sete Cmm s Ea EE CU UG fO a tie fee in ael an x E Fig 4 12 The Help drop down menu 4 7 1 Help Topics Use this command to view the system s Help menu topics im 18 1131 81AA e p36 Screen functions Cu 4 7 2 About IM VDS CL Use this command to display the copyright notice and version number of your copy of ImageMaster VDS CL About IM VDS CL x software VDS CL manager e Version 1 00 Web Site http www apbiotech com e Licensed to Amersham Pharmacia Biotech Fig 4 13 The About IM VDS CL dialogue box 4 8 Spot Filtering Tool There is a tool provided from version 2 01 of the software that allows the noise filtering on images On the VDS CL desktop there is an icon called SP Filter By clicking on this icon a File Open dialogue box pops up Choose the file you will to clean up and press Enter key A new file will be created into the same directory with a similar name and a suffix _SF This application is only removing single high intensity pixels from the image and improves the quality for
5. Contents 4 5 3 Analog Dialog Bar 33 4 5 4 Digital Dialog Bar 0 02000 cee eee 33 4 5 5 Optics Dialog Bar 33 4 5 6 Default View 33 4 6 Presets Menu L 0 0 34 4 6 1 Load Procedure cece ee ee 34 4 6 2 Save Current Procedure As 34 4 6 3 System Presets ee ees 35 4 7 Help Menu uc la a rl 36 4 7 1 Help TOPICS fire Pb pP Pialle 36 4 7 2 About IM VDS CL a 37 4 8 Spot Filtering Tool 00 37 5 Touchpad 5 1 The Taps panel o 39 5 1 1 Learning to tap on the touchpad 39 5 4 2 Pointers Tab aro aL eoe ARSA 40 5 1 3 Assign Buttons on the right hand side of the touch pad 40 Balad TestArea oiii o A Bek MAS ore Be 41 5 1 5 Single Tap Duration a 41 5 1 6 Double Click Speed 41 5 2 Motion Tab pamamasa rra Laga 42 5 2 1 Pointer Trails oo 42 5 2 2 Relative Speed ee 42 5 2 3 Acceleration ooooooccccoo ee ee 42 5 3 Scrolling Tabra cid ne ee A KA 42 5 3 1 Scrolling Method nananana 43 5 3 2 Scrolling Types lille 43 5 3 3 How dol use Scrolling 0oooooooooomo ooo 43 5 4 Options Tab sui le eu IER ANG 43 DAT Autojumps i es bd bene RI qUAS ei 43 5 4 2 Rotation LL 44 5 4 3 Motion extenders 0 00 eee eee 44 5 5 General Tab 000 eee eee eee 45 Contents vili 6 Printer 6 1 Film types and film loading
6. System overview 3 3 Status Bar The status bar is displayed at the bottom of the onscreen window To display or hide the status bar use the Status Bar command in the View menu The left area of the status bar describes actions of menu items as you use the arrow buttons to navigate through menus This area similarly shows messages that describe the actions of toolbar buttons as you depress them before releasing them If after viewing the description of the toolbar button command you wish not to execute the command then release the mouse button while the pointer is off the toolbar button The right areas of the status bar indicates which of the following functions are activated Live Freeze Saving Image indicates the current system video situation Lights indicates the light and light sensors state In case one of the lamps is burned and the matched light is operated the system will state error the light type and the number of the burned lamp Doors indicates if door is open or closes The system will operate lights only if the door is closed Drawer Indicates the drawer type and position epll System overview 3 4 Camera Control Bar The Image Master VDS CL contains the following Dialogue Bars Digital Dialogue Bar Optics Dialogue Bar Analog Dialogue Bar The Dialogue Bars are located on the left portion of the screen and provides a quick access to most of the functions listed under Camera drop down men
7. on the integrated hard disk or to a connected network The system can be directly connected to a 10baseT or 100baseT Network through the network connector on the back panel e pl Unpacking amp Installation EM 2 Unpacking 8 Installation The ImageMaster VDS CL should be handled carefully during the unpacking procedure and care should be taken when installing and connecting the system Please follow the directions outlined below 2 1 Unpacking Procedure Unwrap all packages carefully and compare the contents with the packing list making sure all items arrived Inspect all components for damage that may have occurred while the unit was in transit If any part appears damaged immediately contact the carrier Be sure to keep all packing material for re packing should it become necessary to move the unit Use the following steps to unpack the ImageMaster VDS CL 1 Gently remove the box lid and the upper cardboard foam material 2 Take out all items from the upper foam and put them in a safe place Remove the upper foam damper 3 Take out the monitor box 4 Take out the film holders a pair of green holders near the monitor box 5 Remove the film roll from the foam near its holders 6 Remove the keyboard box and any additional cardboard foam material 7 Remove the surrounding cardboard sleeve Caution Lifting the ImageMaster VDS CL from the shipping box requires at least 2 persons Maintain the instrument
8. 256 125 0 125 256 512 1023 This setting will only affect the displayed or printed image You can adjust the Contrast slider into the Digital dialogue bar on the left part of the screen Contrast o rra agr 4 4 5 Brightness The following Brightness Values are available from the Menu Camera Brightness 0 50 100 125 150 200 255 which is the maximum Brightness value available This setting will only affect the displayed or printed image You can adjust the Brightness slider into the Digital dialogue bar on the left part of the screen Brightness 125 cola T 4 4 6 Sharpness The following Sharpness Values are available from the Menu Camera Sharpness 0 10 20 30 40 50 60 67 which is the maximum sharpness value available This setting will only affect the displayed or printed image You can adjust the Sharpness slider into the Digital dialogue bar on the left part of the screen Sharpness 0 l Or e p29 END Screen functions 4 4 7 Y Gain The following Y Gain Values are available from the Menu Camera Y Gain 10 20 40 100 200 256 400 800 which is the maximum Y Gain value available Another option is Auto Y Gain Camera gt Y Gain gt Auto Y Gain the system will decrease the Y Gain automatically to meet the optimum brightness This setting will only affect the displayed or printed image You can adjust the Y Gain slider into the Digital dialogue bar on the left part of the screen or push the
9. 3 Z zoom function 26
10. Auto Y Gain button Y Gain 256 aaa Auto Y iain 4 4 8 Zoom This function allows the user to quickly zoom in or out in graded steps from wide angle Out to the tightest zoom In The following Zoom Values are available from the Menu Camera Zoom Out 2 3 4 5 In You can adjust the Zoom into the Optics dialogue bar on the left part of the screen zaam cu ale 4 4 9 Auto Focus Use this command to automatically focus the camera Activating Auto Focus initiates several other functions while the focus is set including turning the Upper White Light On However once the auto focus has been achieved the system will return to the previous settings Adjust the Focus through the Camera Auto Focus drop down menu or with the onscreen Focus buttons mx m a 4 4 10 Iris The following Iris Values are available from the Menu Camera lris 1 2 1 4 2 2 8 4 5 6 8 and 11 or from the onscreen Iris combo box Iris 12 gt im 18 1131 81AA e p30 Screen functions Cu 4 4 11 Filter The following Filter options can be chosen either with the Camera Filter drop down menu or with the onscreen Filter combo box UV Low Band Pass 520 nm FWHM 50 nm UV High Band Pass 580 nm FWHM 50 nm Green High Pass 490 nm Blue Low Pass 450 nm Red Low Pass 590 nm Filter no Filter 4 4 12 Gain The following Gain Values are available from the Menu Camera Gain 0 25 50 75 100 125 150 175 2
11. System maintenance Gi 8 System maintenance 8 1 Care and cleaning Use a soft cloth dampened with water or a mild cleaning solution to clean the outside cabinet display and labels Wipe sample trays after each use to remove all residual materials Use only a damp cloth and a mild cleaning solution Do not mark sample the sample tray surface by cutting gel on it or scraping gel away See the printer section Chapter 6 for instructions on cleaning the printer 8 2 Replacing parts 8 2 1 UV Lamps Whenever the lights are turned on the ImageMaster VDS CL system automatically checks that all UV lamps are working To perform a check of the UV lamps 1 Install a UV tray and switch the Lower illumination to ON 2 Check the status bar window for an error message 3 Repeat this procedure for the Upper illumination 4 Replace any non working lamp with an 8W 312 nm standard UV lamp To replace a lamp Turn the system OFF and disconnect the power cord from the mains outlet Remove the sample tray and open the compartment door Note that there are 8 lamps 4 UV and 4 white light ordered as follows from the inside out WL UV WL UV WL UV UV WL see Fig 8 1 Remove the malfunctioning lamp by carefully rotating it about 1 4 turn counterclockwise until the pins align vertically Carefully pull the lamp upward To insert the new lamp hold it so that the metal pins align vertically Place the lamp in the sockets at each
12. devices that can be adjusted with the various options included in software Tapping on the Touchpad s touch surface will simulate button clicks Right clicks can be activated by tapping the upper right corner of the touch surface Left clicks can be activated in the remainder of the pad Drag features can be activated by double tapping and holding your finger on the pad Enable a scrolling method that is compatible with most Windows based applications Enables a scrolling method that is compatible with most 97 compatible applications that support advanced vertical scrolling Office 97 type applications offer not only horizontal and vertical scroll options but also a Zoom control that allows you to increase or decrease the current view in an Office 97 application To scroll vertically lift and place finger on the right edge of the touchpad and glide your finger up or down To scroll horizontally lift and place finger on the lower edge of the touchpad excluding the corners and glide your finger left or right To zoom in or out lift and place finger on the left edge of the touchpad and glide your finger your finger up or down applicable in many 97 applications To go back or forward pages on the world wide web lift and place a finger on the upper edge of the touchpad and glide your finger left or e p45 mo Touchpad ss right Each 1 8 inch movement will activate a page change Note the back forward feat
13. end of the unit and lightly push it into place Rotate the tube about 1 4 turn clockwise until it locks into place e pol ED System maintenance Lamp codes First letter Light type W White U UV C Cut Second letter Lamp location L Lower U Upper Number Lamp number Fig 8 1 Layout of the lamp locations within the system 8 2 2 White light lamps 1 Install a White light tray and switch the Lower illumination to ON 2 Check the status bar window for an error message 3 Repeat this procedure for the Upper illumination im 18 1131 81AA e p62 System maintenance Gi m swm 4 Replace any non working lamp with an 8W white lamp To replace a lamp IMPORTANT Turn the system OFF and disconnect the power cord from the mains outlet Remove the sample tray and open the compartment door Note that there are 8 lamps 4 UV and 4 white light ordered as follows from the inside out WL UV WL UV WL UV UV WL Remove the malfunctioning lamp by carefully rotating it about 1 4 turn counterclockwise until the pins align vertically Carefully pull the lamp upward To insert the new lamp hold it so that the metal pins align vertically Place the lamp in the sockets at each end of the unit and lightly push it into place Rotate the tube about 1 4 turn clockwise until it locks into place 8 2 3 Replacing fuses Fuses do not require maintenance but may fail due to fatigue under normal operating con
14. mains power outlet There is a lithium battery in the computer motherboard This battery is not replaceable by the customer Use only an S B 4amp fuse there is one spare fuse in fuse housing Please be absolutely certain that you are using the correct mains voltage 100 240 VAC 10 50 60 Hz 2 3 2 Installation procedures During system installation follow the steps in order as given below Ji Remove the monitor from its box and place it on its base on the upper right side of the system Attach the monitor power supply connection cables to the monitor power inlet and the system s power outlet Plug the monitor VGA cable into the monitor socket Place the keyboard on the extendable keyboard holder underneath the instrument base plate and plug the keyboard cable into the keyboard socket If the system will be connected to a network plug the network cable into the network connector Unpacking 8 Installation cn Fig 2 2 Layout of the power panel with On Off switch A power inlet B power outlet C and the fuse holder D with spare fuse 6 Attach the power cable to the mains outlet 7 Insert the film into the printer as outlined in Chapter 6 The system is now operational Fig 2 3 Layout of the computer panel showing port connections for the monitor A serial cable B parallel C keyboard D and network E ep7 System overview 3 System overview The ImageMaster VDS CL softwa
15. or click on the Time Lapse toolbar icon and then choose among the following options Exposure Time Exposure Time can be chosen either by directly inputting values in the windows or by using the sliders buttons to the right of each window Number of iterations Use this function to choose how many images will be taken Delay Use the edit box and buttons to choose the time delay between images The minimum time resolution is 1 second Averaged Sum Use this check box to average a series of images into one image in each iteration Sum Images Use this check box to sum your images into one image in each iteration Start When all parameters have been set push this button The system will then display a Save As dialogue box allowing the user to assign a file name image format and directory location Abort use this button to Abort the Time Lapse Save operation Cancel use this button to return to the main menu Time Lapse Image Save x m Exposure Time sec msec Abort po jo A 20 Cancel Number of Iterations E 4 m Delay hour min sec die ale ae lu Fig 4 3 The Time Lapse Image Save dialogue im 18 1131 81AA e p20 EL Note Note Screen functions Cu When using either Incremental Save or Time lapse Image Save two figures in consecutive order will be added to the given file name for each image e g filenamexx tif 4 1 5 Illumination Correction
16. up and down arrow keys to access FUNCTION mode 3 Then select S5 FUNCTION mode 5 by pressing the up arrow key twice b Press the FUNC key two times to access the ID character set The position 0 and character 46 code for F in Fuji film will appear 046 c In this position use the up and down keys to select the desired character code for the first 0 position For A the code should be 041 as shown in the table below Press the FUNC key to accept The next set of character codes automatically appears The table below lists all position and character codes required to program an APBiotech label on each print Character Position Character code 0 41 1 50 2 42 3 69 4 67 5 74 6 65 7 63 8 68 d Press the FUNC key for more than 0 5 s to set the selected values e ps7 ED Printer and exit Check that the film type setting matches the film type in the printer To match the print to the monitor image follow the procedures outlined below for UV transillumination or for white light imaging 6 5 2 UV transillumination procedure 1 2 3 4 Make sure you have chosen the UV sample tray that the sample is in place and that the tray is properly installed Adjust the desired picture size using the Zoom function Adjust the focus using the Focus controls or Auto Focus Print the image by pressing PRINT on the printer While printing the printer display
17. which the touchpad activates the scroll commands If the cursor is in a window that has a scroll bar scroll functions can be activated 5 4 Options Tab If the touchpad control panel is open click on the Options tab now This group of settings allows you to adjust various touchpad features 5 4 1 Autojumps Autojumps are designed to help you save time by automatically moving the cursor to predefined locations when applications or dialogue windows are opened e p43 mo Touchpad The following autojumps are available No Autojumps Default Button The autojump features setting causes the cursor to jump to the default button such as the OK button in the newly opened window or dialogue box System menu box This setting causes the cursor to jump to the system menu box the small icon in the top left corner of a newly opened window Window center This setting causes the cursor to jump to the center of a newly opened window or dialogue box 5 4 2 Rotation Rotation sets the orientation for the direction of motion This feature is useful if you intend to use the touchpad in a different position For example if you prefer to use the touchpad upside down you would click Set Rotation turn the touchpad upside down and move your finger from the bottom of the touch surface to the top This will set the direction of motion to match the way the touchpad is positioned The Use Defaults button is used to set the rotation b
18. 00 225 250 or by choosing a value and then pressing Set on the onscreen Gain controls Ce 4 4 13 Exposure Time This commands allows the user to choose among available exposure times ranging from 20 msec to 20 minutes Adjust these values either with the Camera Exposure Time drop down menu or with the onscreen Exposure Time setting controls Gopridi Tete into Dame 5a Tar mc may Pap ae y 4 4 14 Date and Time Display This menu allows the user to enable or disable the camera s Date and Time Displays 4 4 15 Display Text Use this command to display and remove text from the camera image memory The user may choose between e p31 4 Screen functions On Choosing On prompts the Display Text dialogue box allowing the user to insert text The text is then displayed on the screen and is available on the printout from the thermal printer Off Removes the Text from the camera image memory 4 5 View Menu The View menu allows the user to toggle various screen views on and off thereby optimising the workspace on the screen The View menu contains the following commands Toolbar Status bar Analog Dialog Bar Digital Dialog Bar Optics Dialog Bar Default View Uppa be Light OSO Ele Ed Lubos Cores M Hain Diala e jee a e om pss aha Y Dina dar Bsa a ia y pao biag Ban Fuere Fig 4 10 The View drop down menu im 18 1131 81AA e p32 Screen functions Cu 4 5 1 Tool
19. 4 1 5 Illumination Correction an 21 4 1 6 Save Current Frame as Bitmab 21 4 1 7 Image Viewer i 224 a lios a ci rin 22 4 1 8 Export to Photoshop standard 23 4 1 9 Interpolate ee 23 4 1 10 Optional Text to Saved Images 24 4 1 11 EX BKA eim Rn ai MAEL ANG e Ra 24 4 1 12 System Shut Down 24 4 2 Edit Menu ia AA 25 4 3 Lights Menu coi NENG EIIBWG ANG 25 43 T Highs Of innata 25 4 3 2 Upper Light On Off annn 26 4 3 3 Lower Light On Off o 26 4 3 4 Guide Light On Off o 26 4 3 5 Cut Light On Off oo 27 4 4 Camera Menu esee 27 ALA Freeze ii Ari AA 28 4 4 2 Block System on First Exposure 29 4 4 3 Default Digital Parameters 29 444 Contrast oci lado hig dh aaa ei la 29 4 4 5 Brightness eee 29 4 4 6 Sharpness i din weit i eei 29 LET Gall uestre sio a a Me Me DADANG va 30 44 8 ZOOM na NG NG bee NADA TA KAGAD paw Pha LG A 30 4 4 9 Auto Focus ees 30 PANO Iris 5 ASLEA col ad dece GE aces e 30 Laa FING etr Rent es eR ERE A 31 PAN 2 Galia pata toe GG ub a ia e dede PAGA VE NEG ies 31 4 4 13 Exposure Time cece ee ee 31 4 4 14 Date and Time Display a 31 44 15 Display Text rt ER ERR ala 31 4 5 View Menu cinco e aia ia iia 32 4 5 TOO bareas nee TR ost e Ete es 33 4 5 2 Status Bar ca sa Fiato NG E ox ENS 33
20. 4 Note the instructions on the cleaning sheet Do not touch the cleaning section of the sheet be sure to insert the sheet with the cleaning surface facing up the printed side of the sheet will be against the rubber roller and the matte side will contact the thermal head and take care to align the sheet The positioning line on the cleaning sheet should align along the entire length of the edge just above the rubber roller The sheet must be fed evenly to ensure proper alignment Also make sure that the sheet makes contact with the rubber roller gently edge the sheet down as far as it will go 5 Simultaneously press the FUNC and up arrow keys The cleaning sheet will advance but not pass all the way through As the sheet advances the LED will switch from indicating error 1 to indicating cleaning mode CL3 im 18 1131 81AA e p52 Printer Cn If the cleaning sheet does not begin to feed move the thermal head release lever to the RELEASE position and reposition it Cleaning sheet Align Guide protrusion Lever RELEASE position Rubber roller Fig 6 5 Cleaning the thermal head When the cleaning sheet begins to enter the stacking tray an audible beep will sound and the LED will display cleaning mode CL2 Move the thermal head release lever to the RELEASE position The printer will then eject the cleaning sheet and the LED will first display cleaning mode CL1 and then error 1 for about 30 seconds If the s
21. Filter ECL large size blots 125 0 ECL 5 00 00 No 250 12 None 0 50 Out Filter ECL medium size 125 0 ECL 5 00 00 No 220 12 None 0 50 3 blots Filter ECL small size blots 125 0 ECL 4 00 00 No 200 12 None 0 50 5 Filter ECL Plus Western 125 0 ECL 2 00 00 No 180 1 2 None 0 130 Out large size blots Filter ECL Plus Western 125 20 ECL 1 00 00 No 180 1 22 None 0 130 3 medium size blots Filter ECL Plus Western 125 20 ECL 1 00 00 No 180 1 2 None 0 130 5 small size blots Filter ECF Western large 125 0 UV 0 30 00 UV 0 4 Upper 0 250 out size blots High ECF Western 125 0 UV 0 30 00 UV 0 4 Upper 0 250 3 medium size blots High ECF Western small 125 0 UV 0 30 00 UV 0 4 Upper 0 250 5 size blots High AlkPhos DirectECF 100 50 UV 0 30 00 UV 180 4 Upper 0 450 out large size blot High AlkPhos DirectECF 100 50 UV 0 30 00 UV 180 4 Upper 0 450 3 medium size blot High AlkPhos DirectECF 100 50 UV 0 30 00 UV 180 4 Upper 0 450 5 small size blot High Sypro Ruby gels 125 0 UV 0 01 00 UV 150 12 Lower 0 250 out 20x 18 cm High Sypro Ruby gels 125 0 UV 0 01 00 UV 150 12 Lower 0 250 3 10x 8 cm High EtBr Large gels 100 0 UV 0 05 00 UV 150 4 Lower 0 250 out High EtBr small gels 100 0 UV 0 05 00 UV 150 4 Lower 0 250 4 High e p35 4 Screen functions o r ue x lt Y Y Name Bright Con es ExpTime Filter Gain Iris Light Shp y gain Zoom ness trast ness Coomassie Gels 100 50 White 0 00 080 No 0 4 Lower 0 250 out
22. Illumination correction is used to correct light source aberrations within saved images A second image is generated from the original according to the user defined criteria in the dialogue box The new image is given the same name but with an _FFC suffix To initiate illumination correction select File lllumination Correction or click on the Illumination Correction toolbar icon and then use the following buttons Acquire Image Use this button to choose the name and format of the images you wish to save Flat Field Use this button to acquire the reference image for the flat field calculation Cancel Use this button to return to the main menu Illumination Correction x Acquire Image leuia Press Acquire Image Button when ready Fig 4 4 The Illumination Correction dialogue When using the white light WL drawer the user will be asked to remove the image media and close the door before the FlatField button will be enabled When using the UV drawer replace the image media with the Field Calibrator before the second exposure 4 1 6 Save Current Frame as Bitmab Use this command to save the active frame When you save a bitmap ImageMaster VDS CL displays the Save AS dialog box for you to name your bitmap e p21 END Screen functions 4 1 7 Image Viewer Use this command to view saved images To initiate the Image Viewer select File ImageViewer or click on the Image Viewer toolbar icon Ima
23. Once the spool seats the rubber roller will start rotating Guide the film to the back of the compartment and gently press the middle of the spool just until the film starts to self feed 6 Allow the film to feed until it emerges at the stacking tray and then stop the roller by lifting the thermal head release lever into the PRINT position Film Rubber roller Fig 6 4 Feeding the film through the roller e p49 ED Printer 7 Close the front cover 8 Optional step Reset the counter to zero by simultaneously pressing the up arrow and PRINT keys Note Fach film roll will produce 170 images at the default 4 3 aspect ratio 9 Ifyou are switching to a different film type change the two settings as follows 6 1 2 Film select setting When the printer is in normal or standby state showing the print number press the down arrow key and then also the FUNC key This will switch to a sub menu in which 6 parameters can be set Forward through the menu 5 steps by pressing the FUNC key until either LCr required setting for the Super VDS film or TFF required setting for VDS film appears Press either the down arrow key or the up arrow key to toggle between the two choices Press and hold the FUNC key for longer than 0 5 s to set the current value and then exit 6 1 3 Tone setting When the printer is in normal or standby state press the FUNC key to enter the Print mode submenu Forward through the menu 3 step
24. User Defined Load Procedure User Defined Save Current Procedure As System Presets les 5 ale Conan fi ep ning Doa pra v Digital Dio E bgal a y peer la Fig 4 11 The Presets drop down menu 4 6 1 Load Procedure Use this command to load existing Procedure The ImageMaster VDS CL system will change the current system parameters to the loaded parameters When loading a Procedure the system displays the File Open dialogue box prompting you to choose the stored Procedure to load Note that you cannot reload the same procedure name that the one already in memory 4 6 2 Save Current Procedure As Use this command to save and name the active setting The system displays the Save As dialogue box allowing the user to name the procedure and choose a destination directory for saving To save the current updated procedure with its existing name and directory use the Save command im 18 1131 81AA e p34 Screen functions Cu 46 3 System Presets Use one of the presented system Presets as a first approach to optimise the chosen kit Name Bright Con Drawer ExpTime Filter Gain Iris Light Shrp Y gain Zoom ness trast ness CDP Star large size 125 0 ECL 10 00 00 No 250 1 2 None 0 50 Out blots Filter CDP Star medium 125 0 ECL 5 00 00 No 220 1 2 None 0 50 3 size blots Filter CDP Star small size 125 0 ECL 5 00 00 No 200 12 None 0 50 5 blots
25. ack to the standard setting 5 4 3 Motion extenders Motion extenders help you drag efficiently and easily Motion extenders only work with tap engaged drag functions No extend disables all Motion extenders GlideExtend when enabled virtually eliminates the edge of the pad when dragging For example if you drag an item and hit the edge of the touchpad s surface GlideExtend will hold the drag while you lift and reposition your finger to complete the drag function GlideExtend will time out after 3 seconds GlideExtend can be released at the edge of the pad by tapping once or releasing the drag away from the edge of the pad Drag lock when enabled holds the drag function indefinitely until a primary button is pressed or until you tap on the pad Motion extenders can be found in the touchpad control panel on the Options tab im 18 1131 81AA e p44 Touchpad cn 5 5 General Tab If the touchpad control panel is open click on the General tab This group of options allows you to change pointing device drivers and the current pointing device the device being adjusted by the control panel settings The Name field allows you to change the currently installed pointing device driver Changing the driver from the GlidePoint driver will disable the touchpad control panel returning the standard mouse control panel The Choose a device to customize setting allows you to select a pointing device from a list of currently connected
26. avelengths 6 filters motorized turret Selectable emission filters Green 99 555 nm high pass Blue 99 495 nm high pass Red 99 595 nm high pass UV low 520 nm band pass UV high 580 nm band pass Gel positioning By laser beam Gel cutting facility Yes by additional UV tubes Sample Illumination standard UV 312 nm and white light in reflection or transmission mode Spatial resolution at max imum magnification 4 lp nm Temperature controlled CCD 752 x 582 pixels Lens Motorized for zoom aperture and focus e p65 System Specifications Focus Automatic in any mode Image file format TIFF 12 10 or 8 bits Printout Built in high quality video printer and parallel port Sample drawers Chemiluminescence fluorescence and white light Sample drawer size 21 x 25 cm Voltage 100 240 V Frequency 50 60 Hz Power consumption 300W Dimensions HxWxD 88x80x41 Operating temperature humidity Weight 10 to 30 C max 80 Rh according to IEC 1010 1 Approx 50 kg EMC and Safety CE 89 336 EEC EMC directive CE 73 23 EEC LV directive EN 50081 1 EN 61326 1 IEC EN 61010 1 im 18 1131 81AA e p66 System Accessories GE B System Accessories The following table gives a list of the ImageMaster VDS CL a
27. bar Use this command to display or hide the Toolbar A check mark appears next to the Toolbar menu item when the Toolbar is displayed 4 5 2 Status Bar Use this command to display or hide the Status Bar at the lower right hand corner of the screen A check mark appears next to the menu item when the Status Bar is displayed 4 5 3 Analog Dialog Bar Use this command to display or hide the Analog Dialog Bar at the left of the screen A check mark appears next to the menu item when the Analog Dialog Bar is displayed The Analog Dialog Bar allows to set the Gain value and the Exposure Time 4 5 4 Digital Dialog Bar Use this command to display or hide the Digital Dialog Bar at the left of the screen A check mark appears next to the menu item when the Digital Dialog Bar is displayed The Digital Dialog Bar is used to control the look of the image on screen and on a printer by changing the Contrast Brightness Y Gain and Sharpness 4 5 5 Optics Dialog Bar Use this command to display or hide the Optics Dialog Bar at the left of the screen A check mark appears next to the menu item when the Optics Dialog Bar is displayed The Optics Dialog Bar is used to control the camera optics User can set the focus Iris Filter and Zoom from this dialogue box 4 5 6 Default View Use this command to set the system to default view status e p33 4 Screen functions 4 6 Presets Menu The Presets menu contains the following commands
28. ccessories and their code numbers Description Quantity Code Number CL drawer 1 18 1137 93 UV drawer 1 18 1137 91 White light drawer 1 18 1137 92 VDS film TFF 4 rolls 14m 80 6249 67 Super VDS film HG 4 rolls 14m 80 6249 86 Tube white light 6 W top 2 18 1134 10 Tube UV 6 W 254 nm top 2 18 1134 04 Tube UV 6 W 312 nm top 2 18 1134 05 Tube UV 6 W 360 nm top 2 18 1134 11 Tube white 8 W bottom 2 18 1139 27 Tube UV 8 W 254 nm bottom 2 18 1134 03 Tube UV 8 W 312 nm bottom 2 18 1139 28 Tube UV 8 W 360 nm bottom 2 18 1134 15 Field calibrator 1 18 1137 90 e p67 Index A autofocus 26 C camera control bar display or hide 29 layout 11 camera functions Cut D auto focus 26 contrast 25 date and time display 28 display text 28 exposure time 27 filter 27 freeze 25 gain 27 iris 26 remaining time display 28 sharpness 25 zoom 26 overview 14 default parameters 16 default view reset 29 dialogue E About IM VDS CL 32 Illumination correction 19 Incremental save 17 System shut down 20 Text to add 20 Time lapse image save 18 edit menu copy 21 exposure time settings 27 Index Index ema ii file menu default state 16 export to Photoshop standard 19 illumination correction 18 incremental save 16 optional text 19 save image 16 time lapse save 17 film clearing a jam 45 loading 42 removing 44 setting s
29. ditions Fuses protect equipment by disconnecting loads too large for the instruments circuit design For continued protection replacement fuses must be of the specified voltage and current ratings 4A Slow Blow The fuse holder situated above the main power cord holds two fuses one is a spare To replace the fuse 1 Caution Set the power switch to OFF and detach the power cord before replacing fuses 2 Insert a small flat blade screwdriver into the slot on the fuse holder depress and pull the holder door forward 3 Pull the fuse out of its holder and inspect it If the fuse element is burned or broken replace the fuse with an identical type If the fuse appears to be intact check it with an ohmmeter 4 Insert a good fuse into the holder and press the holder door shut e p63 System Specifications A System Specifications The following table summarizes the specifications for the ImageMaster VDS CL system System specifications are subject to change without notice Instrument Type Thermo electrically cooled CCD dig ital camera with micro lens technol ogy Built in Computer Interfacing Pentium MMX Processor 233Mhz 32 Mb Ram 6 4 Gb Hard drive 48 bit I O card SVGA card 2Mb VRAM Ethernet Serial amp Parallel ports Integrated industry standard PC Pen tium 233 with Ethernet 10 100 Mbits floppy disk amp hard drives RS232 C and parallel output keyboard and mouse W
30. e and choose the image format You then click on the button called Save in the dialogue to execute the save command Contents CONTENTS Preface About this manual eese iii Assumptions iii lie ia iii Typographical conventions aaa iv 1 Introduction 2 Unpacking amp Installation 2 1 Unpacking Procedure 3 2 2 Packing List iii aia a a eases 4 2 2 1 System Components elles 4 2 2 2 DOCUMENTS LL 5 2 2 3 Mariuals is iii i 5 2 3 Installation inci IRI RR RIA 5 2 34 Precautions sary ode RR ERRARE ila RR E 5 2 3 2 Installation procedures 6 3 System overview 3 1 The Screen Interface leseessss 9 3 2 Toolbar siecle nu RE Rr a mese Rub eg 9 3 3 Status Bar unie Eia LIE E 11 3 4 Camera Control Bar eese 12 3 5 Getting Started 2 0 00 12 3 6 On line Help Menu eere 14 3 7 Other system functions 15 IMAN iu oet Dre me TES ees mar m beta tt ee 15 3 7 2 Cutting option 15 3 7 3 Positioning of samples 15 374 Camera KG NG i tear 15 3 7 5 Save Options 15 3 27 61 Filters rese aria barba lb eo 15 Contents vi 4 Screen functions 43 File Menu see eed id re 17 4 1 1 Default State wicca aaa Ga NAA a a HA 18 41 2 Saye Image cele c re Rl RR x Rm 18 4 1 3 Incremental Save 00 19 4 1 4 Time Lapse Save 2 2 20
31. e ler Fig 4 8 The Lights drop down menu 4 3 1 Lights Off Select Lights Lights Off to turn off all lights in the system housing When selected the Lights Off option displays a check mark next to the menu text In addition the lamps box in the Status bar should read Sensors OK e p25 END Screen functions 4 3 2 Upper Light On Off Selecting Lights Upper or clicking on the Upper Light toolbar icon turns the Upper lighting source on or off When another light is on and the user chooses to turn the Upper light on the current light is switched off There are 3 onscreen indicators visible when the Upper light is turned on 1 A check mark is visible next to the Upper menu text 2 The Upper Light icon on the toolbar is depressed 3 The Lamps box in the status bar indicates W Upper OK if the White drawer is used or U Upper OK if the UV drawer is used 4 3 3 Lower Light On Off Selecting Lights Lower or clicking on the Lower Light toolbar icon turns the Lower lighting source on or off When another light is on and the user chooses to turn the Lower light on the current light is switched off There are 3 onscreen indicators visible when the Lower light is turned on 1 A check mark is visible next to the Lower menu text 2 The Lower Light icon on the toolbar is depressed 3 The Lamps box in the status bar indicates W Lower OK if the White drawer is used or U Lower OK if the UV drawer is
32. e tray snaps into place with keys a click Fig 6 1 Layout and functions of the thermal imaging printer e p47 ED Printer Note im 18 1131 81AA e p48 6 1 1 Loading a roll of film This section describes how to install a new roll of film when no film is currently installed or when the film roll is empty To switch to a different type of film first remove the installed film by following the instructions in the next section Removing a roll of film Note the type of film you are installing Super VDS film is included with the unit and the printer is originally set to LCr to process it Regular VDS film can be ordered and if used the setting must be changed to TFF see step 9 below Keep film away from direct sunlight during loading Always store film in a cool dark place 1 The VDS mains power switch must be on the secondary power switch must be turned to ON and the printer power switch must be on 2 Release the printer cover by pressing the PUSH button on the upper left corner Lever in RELEASE position Fig 6 2 Removing an empty roll of film 3 Move the thermal head release lever down to the RELEASE position 4 Lift the film roll out of the holder rails Remove the flanges from both ends of the spool and insert them into the new roll of film Printer Cn Holder rails Film spool RELEASE position Fig 6 3 Loading a new roll of film 5 Seat the new film spool into the holder rails
33. ead release lever down to the RELEASE position Reload the film according to instructions in the Loading a roll of film section above e pSl ED Printer Note Important If the roller is still unable to fed the damaged film turn the power to the printer OFF and lower the lever into the RELEASE position Lift the roll of film out and slowly but firmly pull the film free of the roller Once the film is free cut off the damaged section It may be easier to reload the film if you angle the cut slightly Turn the printer power back ON Reload the film according to instructions in the Loading a roll of film section above It is advisable to clean the roller see below after a jam to remove particles that may streak the film 6 4 Cleaning the Printer 6 4 1 Thermal head It is good practice to run the cleaning sheet after every roll of film to prevent printing quality problems such as blotches and streaks The maximum recommended interval between cleaning sheet runs is three rolls of film approximately 500 prints Use only the accessory cleaning sheet to clean the thermal head 1 Remove the film according to the instructions in the Removing a roll of film section above 2 Lift the roll of film out Turn the printer power OFF and then ON to reset the printer mode The LED will indicate error condition 1 and an audible beep will sound 3 Make sure the thermal head release lever is in the PRINT position
34. election 44 tone setting 44 types 41 filters overview 14 types 27 freeze functions activating 25 on screen indicators 25 G gain settings 27 H help menu online 13 topics 31 update presets 32 I illumination correction 18 installation precautions 5 procedures 6 iris settings 26 L lights cut 23 guide 23 Index lower 23 off 22 replacing UV 55 replacing white 56 upper light 22 M manual assumed skills of user 1 bold typeface entries ii contents overview i P presets load procedure 30 save current procedure as 30 user defined 30 printer cleaning rubber roller 47 cleaning thermal head 46 default parameters 51 film removal 44 film types 41 loading film 42 presets 48 UV procedures 52 white light procedures 52 R replacing parts fuses 57 UV lamps 55 white light lamps 57 S sample cutting 14 positioning 14 save current procedure 30 incremental 16 options 14 time lapse 17 screen functions 15 iii Index iv sharpness settings 25 status bar display or hide 29 system accessories 61 functions 1 presets 31 shut down 20 specifications 59 version number 32 system maintenance cleaning 55 T text display toggle 28 optional image 19 toolbar display or hide 29 icons 9 layout 9 touch pad control panel 33 touchpad general controls 39 motion control 36 options 37 scrolling 36 tapping 33 troubleshooting 53 typographical conventions ii U unpacking procedure
35. ered by either inputting the desired values directly in the windows or by clicking on the sliders to the right of each window End Exposure Time The End Exposure Time can be altered by either inputting the desired values directly in the windows or by clicking on the sliders to the right of each window Multiply Factor Use this Combo Box to choose a multiply factor i e incremental increase for the Exposure Times The user can set the Start and End Exposure Times and then adjust the Multiply Factor to arrive at the proper number of images or iterations Use this Combo Box to choose a multiply factor for the Exposure Time Start From End Exposure Time Use this Check Box to start taking your images from the end of the Exposure Time to the beginning Bing Erani Tire E Far ae 4 Maira 2 peat From End Enmore Tin Ered E periurio T inns Viri ue ruler kb xe s 24 AnkaiEndEspecum Tre Fig 4 2 The Incremental Save Dialogue Box Start Once the desired parameter values have been set use this button to start the Incremental Save process The system will then display Save As dialogue box asking for a File name Directory and image file format Abort Use this button to stop the incremental save process Cancel Use this button to leave the Incremental Save dialogue box e pl9 END Screen functions Note 4 1 4 Time Lapse Save This command is used for time lapse imaging Select File Time Lapse Image Save
36. erial 2 Unpacking and installation 3 System overview System 4 Screen functions Operation 5 Touchpad 6 Printer System 7 Troubleshooting management 8 System Maintenance Appendix A Technical specifications Assumptions Two broad assumptions are made in this manual 1 You should be familiar with the fundamentals of bio imaging For background on any particular imaging function please refer to the appropriate Help Menu which provides more extensive details 2 You should be familiar with the general principles of using Microsoft Windows 95 on your PC Although ImageMaster VDS CL is a self contained program package and does not require any direct interaction by the user with Windows 95 the user interface principles follow the conventions set by Windows 95 programs iii Preface iv Typographical conventions Menu commands the names of dialogue boxes and windows the contents of dialogue boxes and windows and option buttons are written with a bold helvetica typeface Menu commands are written in the order of the menu name and then the command separated by a colon For example Select File Save Image to display the Save Image dialogue Locate the destination drive and folder and enter a file name Click on Save This directs you to click on the File menu and select the command Save Image A dialogue called Save Image is displayed in which you must locate the destination folder for the saved file give the file a nam
37. etting 5 1 6 8 Double Click Speed This option allows you to set the double click speed of the current pointing device This setting affects the touchpad buttons as well as the touchpad tapping features e p4l mo Touchpad Personalize your double click speed by double clicking or double tapping on the Earth icon You can verify your setting in the test area the Moon icon If you would like to set your double click or double tap speed back to it s original setting click on the Use Defaults button 5 2 Motion Tab If the touchpad control panel is open click on the Motion tab now The options that are available in Motion allow you to adjust the pointing device s motion characteristics and pointer trails 5 2 1 Pointer Trails Pointer trails provide visual feedback by leaving a distinctive cursor trail This may help if you have difficulty locating the cursor on your screen You may adjust the length of the trails from short to long The Show pointer trails check box is used to enable or disable the pointer trails option 5 2 2 Relative Speed This adjustment allows you to increase the motion sensitivity of the pointing device The higher the speed setting the faster the cursor moves as you glide your finger across the touchpad This setting is helpful for fine tuning the pointing device s range of motion and responsiveness 5 2 3 Acceleration This adjustment allows you to change the pointing device s accelera
38. f film section above 6 5 Printer pre sets Printer parameters are preset for ImageMaster VDS CL system applications The factory set parameters for the system are listed below for your reference im 18 1131 81AA e p54 Printer Cn cs Menu available by pressing the FUNC key only PRINT mode Gain Brightness b0 Contrast C0 Tone T3 Super VDS film or T7 regular VDS film Gain select G_n G28 The available range for these settings is 9 to 9 However 0 is the default and nominal setting and settings outside the 3 to 3 range are not recommended Menu available by pressing both the FUNC and the down arrow key simultaneously FUNCTION mode 1 Output THr Enhance EoF H size 50 Format F10 H posi HOC V posi U0E Menu available by pressing both the FUNC and the up arrow key simultaneously FUNCTION mode 2 Aspect A43 Int Noint in Odd Even odd Mono color Cro Signal select SA Film select LCr Super VDS film or TFF Regular VDS film e p55 ED Printer Menu available by pressing the FUNC up arrow and down arrow keys simultaneously First set the LED display for S3 with the up arrow and down arrow keys and then press FUNC to access FUNCTION mode 3 Direction d0 Posi Nega PoS Film cutsize C_n Cutter Con Mirror obu Gradation G_8 Menu available by pressing the FUNC up arrow and down a
39. further quantitation or for eventual publication if combined with Interpolation It is recommended to use this feature before using the Interpolate feature in Chapter 4 1 9 e p37 Touchpad cn 5 Touchpad The ImageMaster VDS CL utilizes a Touch Pad interface that allows the user to navigate the on screen menu system You can access the Touch Pad control panel by clicking on the Windows Start button lower left hand corner of screen and then choosing Settings Mouse El GlidePoint On PS 2 Port Properties 2 x Taps Pointers Motion Scrolling Options General C Lefthanded C Custom socian Butang Iv Tap enable Testarea Left click Middle click Right click m Single tap duration Set Test Tap once on the globe to set your wee single tap duration i Use Defaults Double click speed Set Test Double Click on the globe to set your E double click speed 2 Use Defauits Fig 5 1 The Touch Pad control panel 5 1 The Taps panel To configure the taps functions click on the Taps panel These options allow you to adjust functions related to the touchpad s buttons and touchpad tapping features These options can also be used to configure other detected pointing devices 5 1 1 Learning to tap on the touchpad Click double click drag and highlight functions of the left click and right click mouse buttons can be activated by tapping directly on the s
40. geMaster VDS CL system displays the Image Viewer dialog box for the user to choose one of the following Adding an image to the Image Viewer Creating a procedure form the viewed image Print the viewed image in the video printer or a regular printer Change the values of the black and white range Zoom the viewed image In this way the user has the ability to view saved images print the images and create new procedures using the saved images parameters Add Image use this button to add an image to the Image Viewer The selected image will be added to the image s list in upper part of the Dialog Box Create Procedure use this button to create a procedure from the parameters that are listed in the right side of the Dialog Box Remove Image use this button to delete the selected image in the viewer Print use this button to print the image in a regular printer e g ink laser etc connected to the system through the parallel port Video Printer use this button to print the image in the system s video printer Fit to window use this button to fit the image to the viewer s window size Set Values use this button to set the maximum and minimum values of the slider as shown in the matching Edit Boxes Invert use this check box to invert the image Zoom use this check box to change the pointer from a zooming mode to a shifting mode and back Images List use this list to change the image in the v
41. heet becomes stuck because it engaged with the roller too late turn the power to the printer OFF and lower the thermal head release lever into the RELEASE position Slowly but firmly pull the sheet free Go back to step 3 above 6 Repeat steps 3 to 5 as often as required 7 To reload the film see the Loading a roll of film section above 6 4 2 Rubber roller White vertical lines or black spots on your prints may indicate that the rubber roller and or the thermal head require cleaning If cleaning doesn t improve the photo quality contact a qualified service technician to replace the thermal head or roller e p53 ED Printer Remove the film according to the instructions in the Removing a roll of film section above Turn the printer power OFF and then ON to reset the printer mode The LED will indicate error condition 1 and an audible beep will sound Moisten a cotton swab with water to clean the roller do not use gauze or cloth because they may catch in the roller Lever RELEASE position Rubber roller Fig 6 6 Cleaning the rubber roller 4 Press the REPRINT key to start the rubber roller rotating It will rotate for about 30 seconds The LED will first indicate error 1 and then cleaning mode CL 1 Clean the roller with the cotton swab as it rotates Allow the roller to dry Move the thermal head release lever into the RELEASE position Reload the film as described in the Loading a roll o
42. iew window View Window use this window to view the image zoom in and out and shift the image in the window im 18 1131 81AA e p22 Screen functions Cu m sew Parameters List use this list to view the image parameters Those are the parameters that will be used in the created procedure E E Hos EA Tir Image d 111 14 INN i Fig 4 5 The Image Viewer window 4 1 8 Export to Photoshop standard Select File Export to Photoshop standard to export an ImageMaster TIFF file to meet Photoshop 16 bit grayscale file format by changing a tag on the image This is useless if the files are stretched to 16 bits by default In both cases the format is compatible with ImageQuant The new file will be stored in the same directory and is given a PS suffix Note It is not possible to change files not created by the ImageMaster VDS CL 4 1 9 Interpolate Use this command to increase the resolution of an image you selected xd by four twice in the width and twice in the height When you interpolate an image ImageMaster VDS CL display the Open File dialog box for you to choose the file After interpolation completion it saves the image with a suffix of IT to the image name Before using this tool we recommend to use the noise filtering feature as described in Chapter 4 8 This will remove unwanted single pixel noise from the image and enhance the results e p23 END Screen functions 4 1 10 Optional Tex
43. in an upright balanced position follow the directions below with particular care 8 Placing one hand on the groove on the lower side of each end see Fig 2 1 and another hand on the upper back side of the system carefully lift the instrument out of the lower foam and place it on a clean surface in a ventilated room Be careful not to tip the instrument while moving it rather keep it upright or tilted slightly back with the weight against your upper hand ep3 gt Unpacking amp Installation im 18 1131 81AA e p4 Fig 2 1 Proper positioning of hands when moving the system 9 If necessary correct the level of the system by adjusting the extension of the feet 10 Remove the two foam holders from the internal UV drawer before use 2 2 Packing List While unpacking verify that each of the following components was delivered 2 2 1 System Components 1 VDS CL system 2 LCD Monitor AC adaptor for the monitor VGA cable AC adaptor power cord Unpacking amp Installation EM Keyboard Power cable Thermal film Film holders 2 pcs Drawers WL UV CL 3m twisted network cable VN ON A t A WwW Field calibrator 2 2 2 Documents 1 Microsoft license 2 2 8 Manuals 1 User manual 2 Monitor manual 3 Printer manual 2 3 Installation 2 3 1 Precautions Before installing the system make sure that the following points are considered carefully Under normal operation
44. manual bio imaging documentation ImageMaster VDS CL multi purpose imaging system for biological applications Amersham im 18 1149 01AA 2000 11 ai Biosciences to order Asia Pacific Tel 852 2811 8693 Fax 852 2811 5251 Australasia Tel 61 29894 5152 Fax 61 298997511 Austria Tel 01 576 0616 20 Fax 01576 0616 27 Belgium Tel 0800 73 888 Fax 03 272 1637 Canada Tel 1 800 463 5800 Fax 1800 567 1008 Central East South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 Denmark Tel 45 162400 Fax 45 16 2424 Finland Tel 095123940 Fax 095121710 France Tel 0169356700 Fax 0169419677 Germany Tel 0761 4903 401 Fax 0761 4903 405 Italy Tel 02 27322 1 Fax 02 27302 212 Japan Tel 813 5331 9336 Fax 81 3 5331 9370 LatinAmerica Tel 55 11 3667 5700 Fax 55 11 3667 87 99 Middle East and Africa Tel 30 1 96 00 687 Fax 30 1 96 00 693 Netherlands Tel 0165 580 410 Fax 0165 580 401 Norway Tel 2318 5800 Fax 2318 6800 Portugal Tel 01 417 7035 Fax 01 417 3184 Russian Federation Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 South East Asia Tel 60 3 724 2080 Fax 60 3 724 2090 Spain Tel 93 594 49 50 Fax 93 594 49 55 Sweden Tel 018 612 00 00 Fax 0186121200 Switzerland Tel 01 802 81 50 Fax 01 802 81 51 UK Tel 0800 616 928 Fax 0800 616 927 USA Tel 1 800 526 3593 Fax 1 800 329 3593 ImageMaster Multiphor PhastGel and ImageQuant are trademarks of Amersham Biosciences Limited or its subsidiaries Amersham Bioscience
45. mise the positioning Symptom The Touchpad does not function 1 Check that the mouse driver is properly set up Symptom The computer does not detect the PS2 1 Check the computer default setting Symptom The system and monitor work but there is no image on the monitor 1 Make sure the connector RGB from the monitor is connected e p59 Troubleshooting 2 Check that the monitor s green lamp is on 3 Check the power cable to the monitor Symptom The computer does not detect the keyboard 1 Check that the keyboard connector is properly connected 2 Check the computer default setting for the keyboard Printer problems Symptom After pressing the PRINT key no film emerges 1 Turn the printer power OFF Check that the film is properly loaded 2 Ifthe printer is out of film reload Symptom Printout is spotty 1 Send a cleaning sheet through the printer to clean the thermal head Symptom Printout quality is poor 1 Check the printer settings there are two film types and the proper setting must be chosen 2 Store the film in a cool dry place to preserve film quality Symptom Error message 1 Refer to the separate Fuji Thermal Imager instruction manual accompanying the ImageMaster VDS CL system It contains an error code key on page 16 2 Reset the printer by turning the printer power switch OFF and then press the DOWN arrow the PRINT button then press ON im 18 1131 81AA e p60 IMPORTANT
46. o step 2 of the Cleaning the rubber roller section below If cleaning the thermal head go to step 2 of the Cleaning the thermal head section below 6 3 Clearing a film jam Allow the printer to rewind the film never pull the film out while the printer is ON or while the lever is in the PRINT position 1 The VDS mains power switch must be on the secondary power switch must be switched to ON and the printer power switch must be ON Release the printer cover by pressing the PUSH button on the upper left corner 2 Inspect the spool for proper placement the film path for obstructions and the film for signs of bunching or buckling To remove a damaged section of film lower the thermal head release lever into the RELEASE position and allow the roller to advance the film into the stacking tray If the printer is unable to feed the film try rewinding the film as follows Return the lever to the PRINT position and switch the printer power OFF Simultaneously hold down both the PRINT and FUNC keys and turn the printer power switch ON The LED will indicate SEV If a section of film is stuck in the exit pressing the down arrow key in the SEV mode will trim it off To rewind the film apply gentle pressure to the film drum to help feed the film at the proper rate and then press the FUNC key to start rewinding The LED will indicate PrU Printer unwind Once the film is free release the FUNC key Move the thermal h
47. of the VDS CL system the user is not exposed to UV light All UV lamps automatically turn off when any door is opened or when the sample tray is pulled out Exposure to UV light is harmful If you choose to operate the UV Cutting option 360nm put the UV drawer in the cutting position and be sure to wear UV blocking glasses to shield your eyes Due to the danger of exposure to high voltage the rear panels should only be removed by trained service technicians The AC power outlet must be properly grounded to protect both personnel and the equipment All devices must be turned off before connecting to any other device such as a computer Making connections with devices turned on may damage both devices eps ED Unpacking amp Installation im 18 1131 81AA e p6 Shut down the system properly according to Windows 95 procedures Disconnecting the system prematurely may result in damaged system files Store film in a cool place away from sunlight Do not leave objects on the top cover as it may interfere with proper ventilation Do not look directly at the laser source and avoid positioning your head directly within sight of the laser while the door is open Locate instrument at a minimum distance of 30cm from the wall and ceiling The ideal operating conditions for the instrument are 25 C 78 F and 55 relative humidity recommended Do not replace UV and WL lamps while the instrument is connected to the
48. on of each parameter is Sharpness 0 Gain 0 Contrast 0 Iris 1 2 Filter No Filter Exposure Time 20 msec Zoom Out Procedure Name Default Brightness 125 Y Gain 256 User Defined Text To Add To Image None 4 1 2 Save Image Use this command to save the active image When you save an image ImageMaster VDS CL displays the Save As dialogue box for you to name your image Images can be saved in TIFF tif formats in 8 10 or 12 bit resolution and can be also stretched to 16 bits by default The following options allow you to specify the name and location of the file you re about to save File Name Type a new filename to save a document with a different name The system supports long file names using Windows 95 standards and an extension of up to three characters ImageMaster VDS CL adds the extension you specify in the Save File As Type box Drives Select the drive in which you want to store the document Directories Select the directory in which you want to store the document Network Choose this button to connect to a network location assigning it a new drive letter im 18 1131 81AA e p18 Screen functions Cu 4 1 3 Incremental Save Use this command to save images with incremented exposure times Select File Incremental Save or click on the Incremental Save toolbar icon and then choose among the following options Starting Exposure Time The Starting Exposure Time can be alt
49. osure time Default Digital Parameters Date and Time Display and Display Text all of the camera functions can also be accessed directly in the camera function box on the left side of the main screen e p27 4 Screen functions Feo Eemi fate dale bg Fig 4 9 The Camera drop down menu 4 4 1 Freeze The Freeze command can be accessed either by selecting Camera Freeze or by clicking on the Freeze toolbar icon When any of the following functions are used the system automatically employs the Freeze command Setting the Exposure Time Setting the Gain Setting a new Procedure Setting a new Preset Activating Default State There are 2 onscreen indicators alerting the user that the Freeze function is on 1 A check mark appears next to the Freeze menu item in the Camera menu 2 The Freeze icon on the Toolbar is pushed in im 18 1131 81AA e p28 Screen functions Cu 4 4 2 Block System on First Exposure Use this command to block the system on every time a new exposure time is set When Block is on ImageMaster VDS CL system will display Blocking Dialog box allowing the user to cancel the blocking 44 3 Default Digital Parameters Use this command to reset all digital parameters to their default values i e Contrast 0 Brightness 125 Sharpness 0 and Y Gain 256 4 4 4 Contrast The following Contrast Values are available from the Menu Camera Contrast 1023 512
50. pad control panel is open 1 Click the Taps tab and click Custom 2 Click Assign Buttons 3 Press a touchpad button of your choosing 4 Select a function for example select double click im 18 1131 81AA e p40 Touchpad cn 5 Click Finish 6 Click Apply to store your settings The button that you selected should now function as a double click button To test it out place your cursor on an application icon and press the button you selected as double click 5 1 4 Test Area The test area is designed to quickly display your current button configuration By putting your cursor in the Test Area portion of the touchpad control panel and pressing the touchpad s buttons or taps you will get an indication of your current button configuration Below are the standard mouse button functions and what they do in Windows 95 Left click Primary is used to open applications drag and select items Right click Secondary is used for extended menu functions and special drag operations Middle click Auxiliary is currently an application specific function 5 1 5 Single Tap Duration This option allows you to set the single tap duration of the touchpad Personalize your single tap speed and duration by tapping once on the Earth icon the Earth icon should spin You can verify your setting in the test area the Moon icon Click on the Use Defaults button to set your single tap duration back to its original s
51. re was designed for advanced imaging functionality within a user friendly environment This chapter provides a brief overview of the system layout and functions 3 1 The Screen Interface All system functions are controlled from the screen Details of the system functions can be found in the following chapters Users should be familiar with the Windows 95 interface and functions Two aspects of the screen interface the Toolbar and the Status bar are described in more detail below 3 2 Toolbar The toolbar is displayed across the top of the application window below the menu bar The toolbar provides quick mouse access to many tools used in the ImageMaster VDS CL Was hzree a ame med PARAR a a uai Fram Mom li forte Fig 3 1 The screen Interface with Toolbar and Status bar To hide or display the Toolbar choose Toolbar from the View menu ALT V T The following items are available on the toolbar e p9 System overview im 18 1131 81AA e p10 Function Default State Load Procedure Save Current Procedure as Upper Light On Off Lower Light On Off Guide Light On Off Cut Light On Off Copy Freeze Lap Time Save Incremental Save Save Image Illumination correction Save image as Bitmap Interpolate Image Viewer o o o o o o 3 a al e ll 8 A EGO Tal Keyboard shortcut Ctrl N gt lt Ctrl O gt lt Ctrl C gt
52. rious filters for documentation of polyacrylamide gels Coomassie blue silver stained gels membranes films blots thin layer chromatography TLC plates autoradiographs and more The tray area is 21 x 25 cm with a maximum image transmission size of 17 5 x 21 cm This accommodates the developed area of one full size Multiphor gel 8 mini gels or up to 15 PhastGel gels The imaging system comprises a highly sensitive charged coupled device CCD camera connected to a monitor which allows for image viewing and manipulation of camera controls before the image is printed or saved A Pentium computer controls the system and the interface is based on Windows 95 The camera lens lights filters and all other functions are controlled automatically This imaging system is contained in a free standing dark room that includes UV and white light sources for obtaining images in transmitted or reflected light conditions Once the image is optimised it can be printed on the integrated thermal printer Two grades of film are available the higher grade Super VDS film is thermally activated and fixed and the regular VDS film is thermally activated and UV light fixed Both use a polymer emulsion technology that produces a sharp image on tough plastic and both have a storage life of 2 years under proper storage conditions temperature of 10 to 23 C and relative humidity of 45 15 In addition the information can be stored on a floppy disk
53. rrow keys simultaneously First set the LED display for S4 with the up arrow and down arrow keys and then press FUNC to access FUNCTION mode 4 LED display select d50 Print no print Con T H temp print ToF EPDT 9A8 VSDT 5Cd Filter select 5F1 Menu available by pressing the FUNC up arrow and down arrow keys simultaneously First set the LED display for S5 with the up and down arrow keys and then press FUNC to access FUNCTION mode 5 ID print Pon ID character set 050 Note The 16 available characters are programmed at the factory to spell APBiotech To re program see the Fuji Thermal Imager manual im 18 1131 81AA e p56 Printer Cn 6 5 1 Restoring default printer parameters If the printer settings are in an unknown state restore default parameters according to the instructions below For a full description of all printer options and settings see the Fuji Thermal Imager instruction manual accompanying the ImageMaster VDS CL system 1 To initialize all printer parameters hold down both the down arrow and PRINT keys while turning the printer mains power switch ON After printer initialization step 1 each print will be labelled Fuji film To program the 16 character label to read APBiotech follow the procedure below For additional codes to program your preferred text refer to the Fuji printer manual a Press the FUNC key while holding down both the
54. s P and does not accept further commands Once the print number displays the printer is ready to print 6 5 3 White light imaging procedure 1 um 18 1131 81AA e p58 Make sure you have chosen the while sample tray that the sample is in place and that the tray is properly installed Adjust the desired picture size using the Zoom function Adjust the Focus using the Focus controls or Auto Focus Print the image by pressing PRINT on the printer While printing the printer displays P and does not accept further commands Once the print number displays the printer is ready to print Troubleshooting 7 Troubleshooting This section provides advice on steps to be taken if you encounter problems with the operation of the ImageMaster system Symptom The system will not turn on 1 Check that the main power switch is ON 2 Check that the mains power inlet is properly connected 3 Check the main fuse an extra fuse is located in the fuse holder Symptom The monitor does not function 1 Check that the monitor power switch is ON 2 Check the power inlet to the monitor Symptom The printer does not function 1 Check that the printer power switch is ON Symptom One of the lower upper or cutting lamps does not function 1 Check that the lamp is properly seated in it s socket Symptom The laser pointer does not function 1 Check that the drawer is in the Guide position pull the drawer out to opti
55. s by pressing the FUNC key until either T3 recommended setting for Super VDS film or T7 recommended setting for VDS film appears Please note that the setting that results in the closest approximation of the image on the monitor may vary slightly from these values Press either the down arrow key or the up arrow key to scroll through the tone setting options until the desired setting appears Press and hold the FUNC key for longer than 0 5 s to set the current value and then exit 6 2 Removing a roll of film Allow the printer to rewind the film never pull the film out while the printer is ON or while the lever is in the Print position 1 Set the printer to service mode The ImageMaster VDS mains power switch must be ON the secondary power switch must be ON and the printer power switch must be OFF Simultaneously hold down both the PRINT and FUNC keys and turn the printer power switch ON The LED will indicate SEV im 18 1131 81AA e p50 Note Printer Gi 2 Release the printer cover by pressing the PUSH button on the upper left corner of the cover 3 To rewind the film press the FUNC key If necessary apply gentle pressure to the film drum to help feed the film at the proper rate The LED will indicate PrU Printer unwind Once the film edge is free release the FUNC key 4 If loading a new roll of film Go to step 3 of the Loading a new roll of film section above If cleaning the printer roller go t
56. s is a trademark of Amersham plc Microsoft and Windows 95 are trademarks of the Microsoft Corporation Pentium is a trademark of the Intel Corporation Fuji is a trademark of the Fuji Photo Film Co Ltd Photoshop is a trademark of Adobe Systems Incorporated Amersham Biosciences Bj rkgatan 30 SE 751 84 Uppsala Sweden Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA England Amersham Biosciences 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group that supplies them A copy of these terms and conditions is available on request Amersham Biosciences 2000 All rights reserved Preface Preface About this manual This manual provides a full reference to the ImageMaster VDS CL bio imaging system ImageMaster VDS CL together with the ImageMaster analysis software is a complete package for documentation and analysis of sample images in visible light fluorescence and chemiluminescence ImageMaster utilizes software that runs on an integrated PC compatible along with an Intel processor under Microsoft M Windows 95M and hardware for interfacing the controlling PC to the imaging system The manual is organised in 8 chapters and 1 appendix Introductory 1 Introduction mat
57. save files in a variety of formats including 8 10 12 16 bit images in TIFF formats In addition the Incremental Save and Lap Time Save functions allow the user to save and view images in different time increments thus allowing for the optimisation of image quality 3 7 6 Filters The user can control a filter wheel automatically and depending on the desired result choose various filter colours For Chemiluminescent work use the CL filter which means No Filter is employed e pls Screen functions CU 4 Screen functions Most of the system functions for the ImageMaster VDS CL system are available within one of the drop down menus This summary will lead you through the following onscreen menus File Edit Lights Camera View Presets Help 4 1 File Menu LO Ar e xam A S Fig 4 1 The File drop down menu The File menu is used to organize and save the results of your analysis and documentation The drop down menu consists of the following options ep17 END Screen functions 4 1 1 Default State Set the system to the initial position of all parameters When turning the ImageMaster VDS CL system on not through user defined procedure it will automatically be set to default state After manipulating the settings of an image the user can return to the default state by selecting File Default State or by selecting the Default State Shortcut lt CTRL N gt The default positi
58. t to Saved Images Select File Optional Text to Saved Images to add text to the bottom of the image in the saved file The text is added without altering the original file information FF Const F Gan E Poda haa F Brghina F Coy Tira F Y Gan F Za F la Salaci Ki elect Hone Fig 4 6 The Text to Add dialogue box 4 1 11 Exit Choose File Exit to end your ImageMaster VDS CL session without shutting down the system Mouse Double click the application s Control menu button on the upper right corner Fig 4 7 The window Exit button Keyboard lt ALT F4 gt 4 1 12 System Shut Down The System Shut Down dialogue is displaed when the user chooses File System Shut Down The system will always prompt the user to save any opened but unsaved documents Choose the Yes button to shut down the system or the No button to return to the previous window im 18 1131 81AA e p24 Screen functions CU Note It is important to wait for the It is now safe to turn off your computer prompt before turning off the mains power to your system Turning off the power before this dialogue appears may damage system files 4 2 Edit Menu The Edit menu contains the Copy command Select Edit Copy or click on the Copy toolbar icon to copy the current image onto the clipboard Copying an image to the clipboard erases any data that were previously stored there Keyboard lt CTRL C gt 4 3 Lights Menu Tort al Ligas Fem Wiek el
59. tion Acceleration allows the cursor to move faster on the screen with increased movement from the pointing device Acceleration increases in increments depending on how the adjustment is set Acceleration allows quick cursor movement while maintaining cursor accuracy with small amounts of movement Setting acceleration to none disables the acceleration feature 5 3 Scrolling Tab If the touchpad control panel is open click on the Scrolling tab now These options control the available methods of scrolling The touchpad activated scrolling is compatible with many Windows applications including 97 applications that support advanced scrolling and zooming im 18 1131 81AA e p42 Touchpad cn Scrolling also incorporates audible feedback that may be applied to each of the scrolling options and types 5 3 1 Scrolling Method These settings allow you to choose the scrolling method to use None disables the touchpad scrolling options 5 3 2 Scrolling Types This group of options allows you to enable or disable specific scrolling functions You can enable or disable the following Vertical Horizontal Zoom Back forward 5 3 3 How dol use Scrolling Scrolling enables you to quickly access the scroll bars in most Windows applications Zoom in many 97 applications and move Back and Forward in many applications such as web browsers The touchpad surface displays vertical and horizontal arrows that designate the areas in
60. u As the functions of the Digital Analog and Optics Dialogue bars are dealt with in detail in Chapter 4 the user is referred to that chapter for a detailed description of the functions Casa p yaa E err dano Fin DA kk kk ki Fig 3 2 The Camera Control Dialogue Bar 3 5 Getting Started These directions are designed as a quick introduction to the initial steps involved in acquiring an image After setting up the system and turning it on see Chapter 2 follow the steps below 1 Check to make sure the system is not in Freeze state the left panel in the status bar should read Live 2 Move into Default state using the Default toolbar icon im 18 1131 81AA e p12 10 11 12 13 14 15 System overview Unfreeze the image using the Freeze toolbar icon Open the door and pull the current drawer out to the edge The drawer must be lifted gently at the end and fixed into position The right most pane in the Status bar should read Guide Place your sample in the middle of the drawer For the positioning of the sample use the Guide light toolbar icon Insert the drawer in the chamber but do not close the chamber door Increase the Gain value in the Analog Dialogue bar to 200 and press Set to set it Unfreeze the image using the Freeze toolbar icon Set the camera Zoom in the Optics Dialogue bar to fit the size of the sample the less irrelevant space in the image the better the
61. ure also may be used in some applications such as time management software for moving from day to day im 18 1131 81AA e p46 Printer Cn 6 Printer The ImageMaster VDS CL utilizes a Fuji Thermal Imaging System to produce printouts of user selected images A complete technical reference to the printer is contained in the accompanying Printer technical manual This chapter is designed as a quick start review of the printer s basic operating procedures 6 1 Film types and film loading Two types of film are available for the ImageMaster VDS CL system The high grade Super VDS film is installed at the factory and the printer is set to process this thermally fixed film The regular grade film is UV fixed so the Film Select setting must be adjusted if switching film types Latch for opening and closing the front panel for film loading and roller cleaning Printer power switch FUJIFILM C THERMAL IMAGING SYSTEM FTI 500 i Press the REPRINT key for additional copies of the image displayed on the monitor Press the PRINT key for a print of the image displayed on the monitor Oooo 0000000 000 OO OR FUNC REPRINT PRINT The FUNC key accesses all the printer settings The arrow keys increase or decrease the value displayed on the LED The LED displays Slide the stacking tray into print number or this slot holding the tray the printer settings level When properly installed ifusing the FUNC th
62. urface of the pad Remember the touchpad responds best to a light quick tap e p39 mo Touchpad To click lightly and quickly tap the surface of the pad once To double click rapidly tap on the pad twice To drag draw or highlight double tap and hold your finger down on the second tap Then glide your finger to move To drag further than the touchpad surface simply lift your finger and reposition after reaching the textured edge of the pad 5 1 2 Pointers Tab If the touchpad control panel is open click on the Pointer tab now This group of options allows you to change the pointer s appearance This section is identical to the Microsoft Pointer options To change all of the pointers at once select a different scheme To change the appearance of a specific pointer click on the pointer to be changed and select Browse to locate other pointer styles 5 1 3 Assign Buttons on the right hand side of the touch pad This option becomes available when the Custom option has been selected When you click on Assign Buttons you will be asked what button or tap you would like to assign You can choose any of the available buttons and can also use the Ctrl key on your keyboard plus any of the touchpad buttons or tapping features Once you have selected a button or tap you will be asked to select a function to attach to the selected button or tap Follow the steps below to make an assignment Make sure the touch
63. used 4 3 4 Guide Light On Off Selecting Lights Guide or clicking on the Guide Light toolbar icon turns the laser pointer on or off This laser pointer is only available when the drawer is positioned above the Cut light and the Drawer panel in the status bar reads Guide There are 3 onscreen indicators visible when the Guide light is turned on 1 A check mark is visible next to the Guide menu text 2 The Guide Light icon on the toolbar is depressed 3 The Lamps box in the status bar indicates Guide OK im 18 1131 81AA e p26 Note Screen functions Cu 4 3 5 Cut Light On Off Selecting Lights Cut or clicking on the Cut Light toolbar icon turns the Cut light on or off This cut light is only available when the drawer is positioned above the Cut lamps and the Drawer panel in the status bar reads Cut amp Guide There are 3 onscreen indicators visible when the Cut light is turned on 4 A check mark is visible next to the Cut menu text 5 The Cut Light icon on the toolbar is depressed 6 The Lamps box in the status bar indicates Cut OK 4 4 Camera Menu The Camera menu contains the following commands Freeze Block the system on the first exposure time Default Digital Parameters Contrast Brightness Sharpness Y Gain Zoom Auto Focus Iris Filter Gain Exposure Time Date and Time Display Display Text With the exceptions of Freeze Block the system on the first exp

ImageMaster VDS-CL - GE Healthcare Life Sciences

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