Hardware User`s Manual Tail
Contents
1. Tail flick meter 18 Harvard Apparatus Panlab H 14 IROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION een dossinobela tun e Check the condition of the fuses The equipment does not send data to the program Sedacom By pressing START STOP button or pedal lamp is not lit When the animal removes the tail the lamp does not turn off FOCUS selector cannot select values below 10 Can calibrate the lamp Tail flick meter Check that the cable is connected between the RS 232 device and the PC serial port see Figure 9 Make sure that both the serial port as the device selected in the Sedacom program are correct The equipment will only send data when after the experiment starts the lamp turns off because the animal has removed the tail If the lamp turns off because has reached the safety time see Chapter 13 will not send data Check that the cable is connected to the lamp see Figure 9 If it starts counting the time but the lamp does not light check the condition of the lamp If the two items are correct it could be a fault in the membrane button Verify that the V shaped accessory is properly seated in Make sure the hole is clean in the V shaped accessory if it is clogged with dirt will not allow the passage of light Clean the phototransistor located in the lamp if it is dirt can prevent light to be detected There is not an err2. the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Tail flick meter Harvard Apparatus Panlab H 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during
3. materiais dos seus velhos aparelhos esta a fazer uma enorme contribuic o para a protecc o do ambiente P Tail flick meter 23
4. ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique D Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt p O Informa
5. 0 21 Harvard Apparatus Panlab 6 INTRODUCTION The LE 7106 Light Beam Analgesy meter is designed to provide all the necessary technical elements to measure the response of rats and mice to pain stimulation by means of a radiant heat applied to their tails AMLGESY METER 1g 7108 Figure 5 LE 7106 Light Beam Analgesy Meter The analgesy meter has an adjustable intensity heat source a photoelectric based animal response detector and a response latency chronometer with 0 01 second resolution The analgesy meter is standard equipped with a maximum stimulus duration of 20 seconds This prevents tissue damage due to excessively prolonged stimulation This time setting can be modified see chapter 13 6 1 SUPPLIED ACCESSORIES e LE 7106 Control Unit 1 e Experimenting Unit 1 e Interconnection cable between Units 1 e Power cord 1 e RS 232 wire 1 e V shaped accessory 1 e Foot switch 1 e Restrainer 1 As option customer is able to order a calibration certificate This certificate is not supplied by default with the instrument This certificate is not free of cost and must be done in factory Tail flick meter Panlab H Ache y 7 EQUIPMENT DESCRIPTION 7 1 CONTROL UNIT FRONT PANEL START STOP TIME FOCUS ANALGE Y METER LE 7106 Tim Sec Figure 6 Control Unit Front Panel e START STOP The lamp is turned on by pressing this button If it were necessary to turn of
6. Focus 50 249000 Focus 50 1 5 2 d depending on the focus and time DIMENSIONS Width x Height x Depth 232mm 111mm 297mm Weight 3 65kg Tail flick meter 21 Panlab H Harvard DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direcci n del fabricante Energ a 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto LIGHT BEAM ANALGESYMETER Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 7106 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tension Low Voltage Basse tension 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electronicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restriccion de ciertas Sustancias Pel
7. Hardware User s Manual Tail flick meter Tail flick test References LE7106 76 0293 Version V29 10 2014X Panlab H Harvard Apparatus Panlab 5 1 0 C Energia 112 08940 Cornella de LI Barcelona Spain www panlab com International Calls 34 934 750 697 Domestic Call 934 190 709 Fax 34 934 750 699 Info Qpanlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Harvard Apparatus Panlab H 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can messenger e ue Pee AN exposed to a hazard MT MEM A touched Feo REEL Y operative life Waste Electrical and Electronic Equipment Directive WEEE w 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure
8. ata will not be sent to the computer e POWER Power inlet main switch and fuse holder Tail flick meter 10 Panlab H Ni 7 3 EXPERIMENTATION UNIT PHOTOTRANSISTOR LAMP RESTRAINER Figure 8 Experimentation Unit PHOTOTRANSISTOR A phototransistor detects light when the animal moves its tail e LAMP Heat is produced by a halogen lamp see technical specifications for further details The lamp holder turns around its vertical axis to withdraw the lamp and facilitate placement of the animal Never turn the lamp on if it is not positioned over the phototransistor as it can produce high temperatures e RESTRAINER Animal is kept in a restrainer There are 2 metal cylinders in which to correctly position the restrainer on the platform ACCESSORY It is strongly advised to work with the accessory at all times It is a V shaped metal unit that must be placed over the phototransistor It protects the phototransistor from ambient light and lamp light It is very important to place the orifice of this piece over the phototransistor see marks on the unit If it is placed in an incorrect position the light will never be detected and time will reach 20 00 The small orifice reduces the amount of light that reaches the phototransistor and makes the system less sensitive to light Tail flick meter 11 Panlab H 8 EQUIPMENT CONNECTION The following figure shows the equipment connection Figure 9 Equipment connect
9. e power inlet Tail flick meter Panlab H 2 Open fuse holder by pulling the flange with a regular A screwdriver Figure 2 Open fuse holder door 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert again fuse holder both possible positions are correct because power supply is universal 6 Ifthe fuses blow again unplug the equipment and contact technical service For electrical safety never open the equipment The power supply has dangerous voltages WARNING Tail flick meter Panlab H 5 TABLE OF CONTENTS 7 1 7 3 10 11 11 1 11 2 12 13 14 15 16 SYMBOLS TABLE GOOD LABORATORY PRACTICE UNPACKING AND EQUIPMENT INSTALATION MAINTENANCE TABLE OF CONTENTS INTRODUCTION SUPPLIED ACCESSORIES EQUIPMENT DESCRIPTION CONTROL UNIT FRONT PANEL CONTROL UNIT REAR PANEL EXPERIMENTATION UNIT EQUIPMENT CONNECTION CHANGING THE LAMP WORKING WITH THE EQUIPMENT RECOMMENDATIONS CLEANING THE RESTRAINER CLEANING THE EXPERIMENTATION UNIT AND V HAPPED ACCESORY SING THE COMMUNICATIONS PROGRAM CHANGING SAFETY TIME TROUBLESHOOTING PREVENTIVE MAINTENANCE TECHNICAL SPECIFICATIONS Tail flick meter Harvard Apparatus 10 11 12 13 14 16 16 16 17 18 19 2
10. eter will run until a tail flick is detected Figure 13 The animal moves its tail 7 Return the lamp to its BACK POSITION 8 Take note of the response time if you are not using SEDACOM to record the experimental data otherwise data are automatically sent to the computer and prepare a new animal repeating the process as many times as necessary Tail flick meter 14 Harvard Apparatus Panlab lt gt Focus 99 ae o ee E o VA AE IIA N AA 10 Temperature 2C 0 5 10 15 Time Sec Figure 14 Temperature versus time at different focus intensity The above graph displays the evolution of temperature versus time with different focus intensities AN warnine The temperature of the lamp can be up to 200 C Tail flick meter 15 Harvard Apparatus Panlab H 11 RECOMMENDATIONS 1 When working with mice as they have very thin tails it is advisable to use low focus values as to prevent harm to their tails 2 When working with rats as they have thick tails it is possible that low focus values will not be able to stimulate the animal In that case it will be necessary to increase the focus value 3 Periodically it will be necessary to clean the experimentation unit and ensure that the accessory V shaped metal unit is correctly positioned 4 Once the animal is placed in the restrainer it will be necessary to wait for 2 minutes for it to calm down T
11. f the lamp before the animal had moved its tail this button can be pressed again When the START button is pressed time will increase until the animal moves its tail At that time the lamp is cut off and time stops e TIME A 4 digit display that counts time while the lamp is on If time reaches 20 00 the system will automatically turn off the lamp and the error message Cut Cut off will be shown on the display this is a safety measure to prevent harm to the animal e FOCUS Decimal selector of heat intensity It can be set from 10 to 99 Tail flick meter Harvar Panlab HA Harvard 7 2 CONTROL UNIT REAR PANEL FOCUS FOOT SWITCH RS 232 POWER LE 7106 Fotus Foot Bwitch Rs 232 v C Made in Spain by Pan ab s l C Rated Voltage 90 260 V AC 47 63 Hz 30 W Figure 7 Control Unit Rear Panel e FOCUS 4 pin connector that sends power to the lamp and sends light detection information from the phototransistor to the control unit e FOOT SWITCH System can be activated remotely using a foot switch It has the same function as the START STOP button on the front panel e RS 232 Data are transmitted through this DB9 connector to the computer by the serial port using the Sedacom software not included should be purchased separately Data are sent to the computer each time a correct test is completed lamp is turned off because the animal moves its tail If a test is finished by pressing STOP or time reaches 20 00 then d
12. h all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Tail flick meter Panlab H Ade 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment check fuses according to the following procedure 1 Remove power cord from th
13. his way false measurements of movements caused by animal stress will be avoided 11 1 CLEANING THE RESTRAINER WARNING In order to clean the restrainer never use neither alcohol nor alcoholic derived products otherwise stripes will appear in the transparent plastic To clean the restrainer you can use a slightly wet cloth and then dry it with a dry cloth If it s too dirty you can wet the cloth with a soapy solution then remove foam with a wet cloth and finally dry it with a dry cloth 11 2 CLEANING THE EXPERIMENTATION UNIT AND V HAPPED ACCESORY To clean the surface of experimentation unit and the V shaped accessory you can use a slightly wet cloth and then dry it with a dry cloth If they re too dirty you can wet the cloth with a soapy solution then remove foam with a wet cloth and finally dry it with a dry cloth Tail flick meter 16 Harvard Apparatus Panlab H 12 SINGTHE COMMUNICATIONS PROGRAM The purchase of the Sedacom software is needed for transferring the data to a computer please contact your local sales delegate for more information The Sedacom software reference is composed by a USB Flash key containing the software Installer a License for use and the Sedacom User s Manual Follow next instructions e Please refer to the Sedacom User s Manual for the instructions about how to install and use the software with the present device e Aserial port RS232 communication cable provided with the present dev
14. ic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment taf Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de a usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente P Remarques concernant la protection de l environnement Conform
15. ice is needed for the connection of the present devices to the computer in which the Sedacom software is installed Please refer to the present User s Manual for the instruction about how to connect this cable to the device e Ifthe computer doesn t have any serial port the RS232 USB adapter is needed ref CONRS232USB contact your local sales delegate for more information WARNING the RS232 communication cable provided with the device is used for any connection of the device with associated software Sedacom etc When the device is used without software in first instance this cable need to be preserved and kept in secure place in case the need of using the system with a software is required in the future In this last case if the user lost the cable a new one should be purchased to his local sales delegate ref CONRS232 The warranty time of this cable is the same than the warranty time of the device Tail flick meter 17 Panlab 13 CHANGING SAFETY TIME If there is no animal response the light automatically cuts off after 20 seconds to prevent harm to the animal s tail This safety time can be modified with internal micro switches as follows 149250961 AREA 32 259184 a u a 20 seg 12 3 4 EIN E m 1559 12 3 4 E u 10 seg 1 2 3 4 5 seg ai NE E 3 Figure 15 Changing safety time
16. igrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les equipements lectriques et lectroniques ROHS 2006 42 EC Directiva mecanica Machinery directive Directive m canique Para su evaluacion se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons applique les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2012 Class B FCC FCC47CFR 15B Class Safety of machinery EN ISO 12100 2010 En consecuencia este producto puede incorporar el marcado CE y FCC Consequently this product can incorporate the CE marking and FCC C En consequence ce produit peut incorporer le marquage CE et FCC En representacion del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canalis k General Manager p Panlab s l u a division of Hakvard BioScience Cornell de Llobregat Spain 15 10 2014 Tail flick meter 22 Harvard Apparatus Panlab H Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domest
17. ion The cables and connections are listed below a FROM CABLE LE7106 FOCUS DBg to 4 pins cable LE7106 FOOT SWITCH FOOT SWITCH 6 35mm Jack mono cable LE7106 RS 232 Computer Com Port RS 232 cable Tail flick meter 12 Harvard Apparatus Panlab 9 CHANGING THE LAMP This type of halogen lamp has a very long service life Nonetheless if you have to change one please follow these instructions 1 Toremove the cover press down and turn it clockwise Do not unscrew Figure 10 Remove the top cover 2 Then proceed to extract the bulb and unscrew the electrical contacts 3 Change the light bulb screw again the contacts and place it in its correct position inside the lamp Figure 11 Unscrew the wires change the lamp 4 Lastly place the cover in its correct position Tail flick meter 13 Panlab tove 10 WORKING WITH THE EQUIPMENT 1 Turn the control unit ON ensuring correct connection between it and the experimentation unit Focus 10 2 Adjust the INTENSITY to the desired heat using the selector 3 Move the lamp to its back position 4 Prepare the animal placing it in the restrainer Then place the restrainer on the platform with the help of the 2 small metal cylinders and leave the animal s tail over the phototransistor Figure 12 Preparation of the animal 5 Move the lamp to its most frontal position 6 Press e The lamp and the chronom
18. or the dial has a brake that prevents selecting values below 10 Lamp cannot be calibrated by the user As option there is a calibration certificate that must be done in factory and it s not cost free 19 Panlab ever 15 PREVENTIVE MAINTENANCE WHEN EXPERIMENT NECESSARY CLEAN V SHAPED ACCESORY CLEAN EXPERIMENTATION UNIT SURFACE RESTRAINER CLEANING CHANGE SAFETY TIME CHECK CABLES CONNECTION See chapter 9 See chapter 13 Tail flick meter 20 Panlab H Harvard 16 TECHNICAL SPECIFICATIONS POWER SUPPLY Input voltage Frequency Fuse Maximum Power Conducted Noise Universal input 100 240V AC 50 60 Hz 2 fuses 5mm 20mm 2 250V Fast 36W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating temperature Operating Relative Humidity Storage temperature 10 C to 40 C 0 to 85 RH non condensing o C to 50 C non condensing COMUNICATIONS OUTPUT Standard Interface RS232C Connector Delta 9 contacts female connector FRONT PANEL Beam Intensity Adjustable in the interval 10 99 Display 4 Digits Max Time 99 99 s limited to 20 seconds Precision 0 015 CONNECTOR FOCUS Pin Function A 5V B Lamp C Input D Lamp CONNECTOR R5232 Pin Function 3 Txd 5 Gnd LAMP Model Halogen OSRAM 64607 Voltage 8V Power 50W Operative life Number of measurements Temperature error never use other brands or lamps 4500 h
19. transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category ll over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Tail flick meter Panlab H ed PC Control Some of these instruments are designed to be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common wit
20. zioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente Nota Protecc o Ambiental Apos implementag o da directiva comunit ria 2002 96 EU sistema legal nacional seguinte aplica se Todos os aparelhos el ctricos e electr nicos nao podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo sao definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instruc es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os
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