PicoPipet User Manual
Contents
1. Canada Bulldog Bio Inc One New Hampshire Ave Suite 125 Portsmouth NH 03801 USA USA phone 1 603 570 4248 fax 1 603 766 0524 China Hua Yue Enterprise Holdings Ltd 483 Xingnan Road Nancun Panyu Guangzhou 511442 phone 86 20 34821111 fax 86 20 34820098 Europe Sonidel Limited 32 Tonlegee Drive Cameron Park Raheny Dublin 5 Republic Ireland phone 353 1 4434358 fax 353 1 6865462 Germany Xceltis GmbH Im Tal 12 74909 Meckesheim Germany phone 49 6226 972418 fax 49 6226 972419 India Medi Analytika India Pvt Ltd 6 Adyar Bridge road Adyar Madras 600 020 India phone 91 44 2446 0988 fax 91 44 2446 3931 Israel Almog Diagnostic P O Box 349 Shoham 73142 Israel phone 972 3 977 3390 fax 972 3 977 3391 Korea Bio Medical Science Co Ltd BMS Bldg 22 Yeoksam ro 7 gil Gangnam ku Seoul 135 936 Korea phone 82 0 2 3471 6500 fax 82 0 2 3471 7001 Singapore Insta BioAnalytik Pte Ltd 21 Biopolis Road 03 01 Nucleos 138567 Singapore phone 65 6515 0110 fax 65 6515 0220 Taiwan Bio East Technology Co Ltd 9F No 21 Fu Kor Rd Shi Lin Taipei 111 Taiwan phone 886 2 2831 3061 fax 886 2 2831 6374 Nepa Gene Co Ltd 3 1 6 Shioyaki Ichikawa Chiba 272 0114 Japan Phone 81 0 47 306 7222 Fax 81 0 47 306 7333 Email info nepagene jp 1 CONTHR 3 PicoPipet Controller NEPA GENE Instruction Manual 201505212. The glass micropipette is connected to the PicoPipet pipette part through the silicone tube Straight shape for dishes microscope slides Cat 1 GS15 10 10 pcs glass micropipettes straight shape tip ID 15 um 1 GT30 10 10 pcs glass micropipettes straight shape tip ID 30 um 1 GT50 10 10 pcs glass micropipettes straight shape tip ID 50 um 1 GT75 10 10 pcs glass micropipettes straight shape tip ID 75 um 2 1 2 Related Products 2 2 S shape for multiple well plates PCR tubes Cat 1 GT30S 5 1 GT50S 5 1 GT75S 5 Description 5 pcs glass micropipettes S shape tip ID 30 um 5 pcs glass micropipettes S shape tip ID 50 um 5 pcs glass micropipettes S shape tip ID 75 um L shape for microdevices or micro liquid applying spotting Cat 1 GS15L 10 1 GT30L 10 1 GT50L 10 1 GT75L 10 Description 10 pcs glass micropipettes L shape tip ID 15 um 10 pcs glass micropipettes L shape tip ID 30 um 10 pcs glass micropipettes L shape tip ID 50 um 10 pcs glass micropipettes L shape tip ID 75 um EM PicoPipet holders 2 A The PicoPipet holder is for holding the pipette part and being attached to a manipulator The light weight helps precise manipulations by a manipulator The position of the pipette part can be flexible because the angle of holding the pipette part can be changed freely There are three different holder lentghs 40 mm 80 mm and 150 mm The 15 mm version has a clamp for fixing the ho
3. DS 5 Push the plunger to push out the residual water and air completely in the pipette part and fill the pipette part with fresh ultrapure water Until air bubbles in the pipette disappear thoroughly keep pushing the plunger You may see water coming from the PicoPipet cable connector as well as the tip of the pipette but it is not a problem Leave it until pushing out the residual water and air in the pipette 4 Preparation of PicoPipet pipette part 6 Connect the cut silicone tube to the tip of the pipette 27 y L Z Push the plunger to push out water and air until they come out PA 8 Connect the glass micropipette to the silicone tube 4 Preparation of PicoPipet pipette part 10 Remove the syringe from the pipette a MT MONOS 4 4 Connecting the PicoPipet cable 1 Connect the PicoPipet cable to the pipette part and the controller 2 Power on the PicoPipet controller 3 Turn the Knob small clockwise to increase Output to 30 Confirm water coming from the tip of the glass micropipette pa 4 Press the Knob small clockwise to change Output to 0 4 4 4 Preparation of PicoPipet pipette part 5 Set the pipette on the PicoPipet holder PicoPipet holder 6 Adjust the position of the tip of the glass micropipette to focus the microscope NOTE Lower the position of the tip of the glass micropipette enough to attach to the bottom lt Good gt lt Not
4. A ee D Ma SAN D 5 1 9 27 UCI di en eee ee 5 1 EA o Neer ee ee Na a a a aa Men en er ee 5 2 OA LEO SOU eek 5 2 5 4 1 Setting a new zero VAIUC cc ccceeceeccceseeeeeccceeeeeeessceeeeeeecscesesess 5 2 5 4 2 Recalling the set zero value oooncnccconnnccoconcncononcnnononcnnonnnnonconanenoss 5 3 55 EQUIP dos 5 3 506 JUMP A einen 5 4 5 01 Senn UMP Vallendar 5 4 5 6 2 Recalling JUMP Valle iii ee 5 4 TS aa san AA ea 5 4 5 7 1 Washing with ultrapure water euunuuesnssneeensennnennennnnnnennnn nennen nenne 5 4 6 SSDECIIGANOINS aaa feet 6 1 7 Wana AA AA AA 7 1 1 Checking the Package 1 Checking the Package Mm 1 x 1 CONTHR 3 PicoPipet controller HN HN Mi Accessories e 1 x PicoPipet cable 1 x Power adapter and power cord e 1 x Instruction Manual this manual 1 1 2 Related Products 2 Related Products EM PicoPipet pipette part It is connected to the PicoPipet controller through the PicoPipet cable This pipette has a special electroosmotic pump that makes it possible to precisely control microfluidic flows without pulsation Cat 1 PPD SP PicoPipet pipette part MW Silicone tubing It is for the connection between the PicoPipet pipette part and a glass micropipette The tubing needs to be cut into a 1 5 cm piece before use The inner diameter is 0 8 mm and the outer diameter is 4 mm Cat i 1 ST D Silicone tubing 1 5 m mi Glass micropipettes
5. GG NEPAGENE 1 CONTHR 3 PicoPipet Controller Instruction Manual DANN TT y Pos r a AED ri fore e e di A EPAGE Step Select Important Information This product is authorized for laboratory research use only not medical product The product has not been qualified or found safe and effective for any human or animal diagnostic or therapeutic application Uses for other than the intended use may be a violation of applicable law The PicoPipet is an electronic ultra small pipetting device that has an electroosmotic pump in its pipette part The special pump can easily control microfluidic flows without pulsation This manual provides instructions for proper setup and operation of the 1 CONTHR 3 PicoPipet Controller and its componenis Table of Contents Table of Contents ImB rtant INTormatllon is i Table Of COE Sen nn nn apaa li 1 Checking th PACK AG C nn nn tai 1 1 2 Related Products sni NA AA ici 2 1 3 Parl NAMES sa oia coa 3 1 4 Preparation of PicoPipet pipette part uuunnuesnnnennnnnnnnennnnnnnnnnn nennen 4 1 ONO A 4 1 4 2 Preparing the silicone tube emma reen arena ana nennen akan anakanak 4 1 4 3 Flushing the PicoPipet pipette part oocccccconcoccccnnnncoconnnccconcnnonnnnonconanoncnnonons 4 1 4 4 Connecting the PicoPipet Cable c ooonncnncccconncnncncnnnncnncnonancnnnnnonancnnnononanennnnos 4 4 5 Operating INSUUCUONS m rida 5 1 Selk ONEVIEW A A
6. a device that controls z axis movements of the PicoPipet pipette part automatically A manipulator can be attached to the horizontal bar of the Z robo 4 different heights can be saved and recalled by touching the buttons on the control panel During the use of the PicoPipet the pipette part need to be moved to fixed heights repeatedly The Zrobo is used to significantly reduce this troublesome manual z axis work The customers who already have the MPP 200A 200B 100 can add the Zrobo to their system and combine the Zrobo with it Description 1 TKRK B1 Zrobo z axis automatic control device 2 Related Products mi Compass video microscope u Compass image The Compass is a video microscope that works by a unique pattern projection method The images of transmissive objects like cells can be high contrast and 3D shape views under the simple optical system With the PicoPipet you can conveniently transfer a cell from one container to another while monitoring the process on the screen The total mafnification is 52x 425x similar to microscope objective 20x Description Compass video microscope EM Compass Z Zrobo mounted video microscope The Zrobo motor that enables automatic z axis movements is mounted on the Compass video microscope The height of the PicoPipet pipette part can be automatically changed to the intended height by touching the button on the control panel If you have the Compass it can be upgraded to t
7. eement is limited to the expense of providing product repair neither Nepa Gene Co Ltd nor its employees or agents shall be liable for indirect special incidental or consequential damages including without limitation business interruption loss of profits or loss of revenues loss of wages or injury to persons property or customers Furthermore Nepa Gene Co Ltd categorically states that the sole and exclusive remedy for nonconforming goods shall be replacement of defective goods or at Nepa Gene s option refund of the purchase price to customer The parties acknowledge that the price of Nepa Gene Co Ltd s products would be much greater if Nepa Gene Co Ltd undertook more extensive liability Any action by customer for any alleged breach of the warranty stated in this section must be brought to the attention of Nepa Gene Co Ltdl within 90 calendar days after the end of the warranty period Notice Products returned to Nepa Gene Co Ltd for repair or replacement shall be received prepaid If found not to be defective under the terms of warranty a charge will be made for repair or replacement and freight costs will be at customer s expense Specifications are subject to change without notice For this reason and at its sole discretion Nepa Gene Co Ltd reserves the right to upgrade products during a repair process Prices for out of warranty repairs are subject to change without notice Contact Area Distributor Address
8. fects in materials and workmanship when used in accordance with their intended use This warranty is limited to twenty four 24 months from the product s original date of shipment from Nepa Gene Co Ltd The warranty for non durable products is limited to thirty 30 days from the product s original date of shipment from Nepa Gene Co Ltd Further this warranty dose not apply to modifications made to the products by the customer or damage to products resulting from modifications or repairs effected by customer or other personnel not authorized by Nepa Gene Co Ltd to perform such work This warranty shall not apply to damage resulting from I loss or damage in transit Il unreasonable use Ill customer s negligence or IV accident Nepa Gene Co Ltd reserves the right to examine the alleged defective goods in order to determine whether the warranty is applicable EM Limited warranty and disclaimer Nepa Gene Co Ltd warrants that all services supplied hereunder will be performed in a workmanlike manner except for the express warranties provided to customer under the above limited warranty for products Nepa Gene Co Ltd makes no other warranties or conditions expressed or implied as to the services or parts supplied hereunder and Nepa Gene Co Ltd expressly disclaims all warranties or conditions of merchant ability or fitness for a particular use EB Limitation of liability Nepa Gene Co Ltd liability under this agr
9. good gt 1 Tip of glass Tip of glass 5 Operating Instructions 5 Operating Instructions 5 1 Overview Basic functions Applied functions Suction Output reverse Discharge Zero set NOTE e Before pipetting the target suction culture media buffer for 1 minute at Output 10 1 2uL to avoid the target being in ultrapure water 5 2 Suction Decrease the Output value on the display by turning Knob large or Knob small counterclockwise e The Output range is from 30 to 30 e The Output increment can be changed by pressing the Step select button Knob large 0 05 0 01 or 0 001 Knob small 0 25 e Abeep sounds when the Output value is minus NOTE During waiting time leaving the PicoPipet without power off be sure to change the Output to the set zero value see 5 4 2 on this manual to avoid extra suction The suctioned targets liquid going into the pump in the pipette may cause damage to the pump 9 1 5 Operating Instructions 5 3 Discharge e Increase the Output value on the display by turning Knob large or Knob small clockwise e The Output range is from 30 to 30 e The Output increment can be changed by pressing the Step select button Knob large 0 05 0 01 or 0 001 Knob small 0 25 5 4 Zero set 5 4 1 Setting a new zero value Small flow in the glass micropipette can arise at the default zero value of Output To achieve the best flow for stopping the target the Output value should be adju
10. he Compass Z Description Compass Z Zrobo mounted video microscope 2 5 3 Part Nam 3 Part Names es EM PicoPipet Controller Top Power connector 1 Zero set button 2 Step select button 3 Jump set button 4 Knob large 1 Zero set button 2 Step select button 3 Jump set button 4 Knob large 5 Knob small Display A Output voltage step 7 A Output voltage step B Offset value C Output voltage value HN Kin Ujana Push Zero Jump The current Output voltage can be set as a new zero value and added to the Offset value The Output voltage increment of Knob large can be changed 0 05 0 01 0 001 The displayed Output voltage value can be saved as Jump value The Output voltage value can be changed by turning the knob Fine adjustment 0 05 0 01 0 001 Output voltage can be reversed by pressing the knob The Output voltage value can be changed by turning the knob Coarse adjustment 0 25 The Output voltage value can be changed to the set zero value by pressing the knob The Output voltage value can be changed to Jump value by pressing the knob when the Output voltage value is zero B Offset value C Output voltage value The current Output voltage increment The increment can be selected from 0 05 0 01 and 0 001 The distance from the default zero value of Output voltage The current Output voltage value that indicates a dis
11. lder to a manipulator Cat 1 PPH4 1 PPH8 1 PPH415 1 Description PicoPipet holder 40 mm PicoPipet holder 40 mm PicoPipet holder 150 mm with clamp 2 Related Products EM Joystick manipulator The joystick manipulator is a manual control micromanipulator that has a joystick bar to intuitively operate the PicoPipet pipette part at XY axis The brass bracket part enables the manipulator shape to be flexibly changed according to the microscope environment Description Joystick manipulator EM Joystick manipulator with lens condenser mounting clamp This includes a clamp that can fix the joystick manipulator on the lens condenser of user s microscope like the picture below The available outer diameter of the lens condenser is 40 70 mm Compared with the magnetic stand kit the next item it saves space and allows easy setting up Description Joystick manipulator with lens condenser mounting clamp 2 3 2 Related Products EM Joystick manipulator with magnetic stand kit This includes poles a magnetic stand and an iron mat that are for the use of the joystick manipulator with user s microscope Compared with the lens condenser mounting clamp the manipulator can be positioned flexibly A place for the iron mat 250 mm x 200 mm is needed next to the microscope Description Joystick manipulator with lens condenser mounting clamp EM Zrobo z axis automatic control device Zrobo is
12. p value set zero value Jump value set zero value Post operation Washing with ultrapure water Power off the PicoPipet controller Disconnect the PicoPipet cable Disconnect the silicone tube and the glass micropipette TIPS When disconnecting the glass micropipette from the silicone tube rotating the glass micropipette helps it detached from the silicone tube smoothly 5 Operating Instructions 4 Connect the syringe to the pipette part mn DODE 5 Push the plunger to push out the residual water medium buffer and air in the pipette part and fill the pipette part with ultrapure water Until air bubbles in the pipette disappear keep pushing the plunger 6 Cover with the reservoir cap Reservoir cap Put the pipette part back into the pipette box 9 9 6 Specifications 6 Specifications Flow Rate Output Voltage Settings Output Voltage Range Input Rating Operating Environment Dimensions Weight 1 nL min to 10 uL min with PicoPipet pipette part Knob large 0 05 0 01 V increments Knob small 0 25 V increments Zero set polarity reverse Jump set 30 to 30 V Power adapter AC100 240V 50 60Hz Temperature 5 40 C Humidity 80 RH Max Non condensing 187 W x 124 D x 46 H mm 0 3 kg 6 1 7 Warranty Warranty EM Limited warranty for products Nepa Gene Co Ltd warrants that its products as delivered shall conform to its specification and be free of de
13. sted Pick up the target 2 Turn Kob large clockwise or counterclockwise to stop the target in the glass micropipette It would be better not to stop the target completely but let the target suctioned just a little 3 Press the Zero set button Then the current Output will be set as a new zero value and added to the Offset value and the values on the display change accordingly 9 2 5 Operating Instructions 5 4 2 Recalling the set zero value When the displayed Output value is not zero press Knob small Then the Output value will be changed to the set zero value and the display will change accordingly e The target liquid in the glass micropipette will stop NOTE During waiting time leaving the PicoPipet without power off be sure to change the Output to the set zero value to avoid extra suction discharge 5 5 Output reverse SON SOHN C a WH DS nen O or at the Output value is changed to the other without change of the absolute value by pressing Knob large 9 3 5 Operating Instructions 5 6 5 6 1 5 6 2 5 7 5 7 1 Jump Setting Jump value DINO e The displayed Output value is saved as Jump value by pressing the Jump set button Recalling Jump value e When the displayed Output value is zero press the Knob small Then the Output value will be changed to Jump value Every time the Knob small pressed the Output value changes as follows Jum
14. tance from the set zero value of Output voltage 3 1 3 Part Names EM PicoPipet Controller Rear DCOUT DCIN35V MAX30V POWER VOLUME Emm REMOTE PicoPipet cable connector Power switch Sound volume control 3 2 4 Preparation of PicoPipet pipette part A4 Preparation of PicoPipet pipette part 4 1 Overview The PicoPipet pipette part must be connected to the PicoPipet controller to do the experiment Before starting the experiment please follow the steps below Flushing the PicoPipet pipette part Connecting the silicone tube and the glass micropipette Connecting the PicoPipet cable 4 2 Preparing the silicone tube 1 Prepare a 1 5 cm long piece of the 1 ST D silicone tubing by cutting out the tubing Mantap aa er 4 3 Flushing the PicoPipet pipette part The residual water and air inside the pipette part need to be flushed out with fresh ultrapure water using a luer lock syringe The special pump part inside the pipette needs to be filled with fresh ultrapure water 1 Prepare the included luer lock syringe or a commercial luer lock syringe e g BD 309657 3mL syringe Terumo SS 02LZ 2 5mL syringe and fill the syringe with ultrapure water 4 Preparation of PicoPipet pipette part 2 Take out the pipette part from the pipette box N A PicoPipet cable connector Reservoir cap 3 Remove the reservoir cap 4 Connect the syringe to the pipette part te eS m
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