Class II, Type B2 (Total Exhaust) Biological Safety
Contents
1. 0 9 meter 3 model one single outlet in workzone 1 2 1 5 and 1610 mm 63 3 080 O 1 8 meter 4 5 amp 6 models two single outlets in workzone 13 Stainless steel armrest 14 Drain valve Retrofit Kit provision 15 Esco Sentinel Gold microprocessor control system 16 Safety glass sliding sash window 17 Single piece stainless steel back wall and side walls 18 Removable side dress panel for plumbing access 19 Pre filter 20 Cabinet power inlet 21 Auxiliary power inlet 22 Zero voltage relay contact 23 Frequency selector lapan cabinet only 24 RS 232 port 300 mm 11 8 o e eese 1540 mm 60 6 E oo 18 970 mm 1270 mm 1570 mm 1870 mm 180mm 7 1 RE 38 2 50 0 61 8 73 6 r ae a E 1115 mm 1420 mm 1725 mm 2030 mm mm 43 9 55 9 67 9 79 9 li 852 mm 33 5 i Accessories and Options Cont as relative humidity in the cabinet ambient e IV bar with hooks Electrical Outlets and Utility Fixtures e Electrical outlet ground fault North America e Electrical outlet Europe Worldwide e Petcock air gas vacuum North America American style Europe Worldwide style DIN 12898 DIN 12919 DIN 3537 Cabinet Accessories e Germicidal UV lamp Controlled by automa2. l Re Le Bcul cure Labculture a i i FIN O Labculture Class Il Type B2 Total Exhaust Biological Safety Cabinet Model LB2 4B_ Class Il Type B2 Total Exhaust Biological Safety Cabinets The Safety Solution for Life Science Laboratories p Main Features Lower exhaust volume and pressure save energy Lower noise for better working comfort Lower height to fit into shorter labs Utilizes exhaust pressure switch for fast and accurate alarm switching Dual airflow sensors to measure exhaust volume and downflow velocity gt Labcultu Esco Sentinel Gold controller with large LCD that simultaneously display key parameters Quickstart mode to turn the blower and lights ON OFF by moving sash to proper position Simplified field calibration mode for easier airflow sensor calibration Voltage free contacts to trigger external alarm external blower damper Built in RS 232 port for remote monitoring of cabinet operation Unique Esco Dynamic Chamber plenum Enhanced Auto Purge side capture zones optimize containment Long life supply ULPA filter per IEST RP CC001 3 with gt 99 999 efficiency for particle size between 0 1 to 0 3 microns a cm a l P lt a Angled supply filter matches cabinet profile to achieve best downflow uniformity Frameless shatterproof sash is easier to clean Ergonomically angled front improves reach and comfo
3. Personal Identification Number can be set to restrict access to main menu Zero Volt Relay Contacts to trigger external alarm Zero Volt Relay Contacts to synchronize building exhaust blower with internal blower SET button doubles as Alarm mute button with adjustable Color coded indicator lamps display green for primary function Touchpad data entry buttons permit control settings and access to diagnostics default A graphical interface indicates cabinet settings and hierarchical ringback period fan operation blue Programmable performance menus for secondary function automatic UV light fluorescent lights and timer simplifies Digital read out with alpha numeric display Indicates all input status and alarm functions operation enhances contamination control extends UV lamp life and saves energy electrical outlet and orange for caution UV lamp ON Diagnostics button to easily check the cabinet operating parameters and assist Servicing All functions can be user activated through touch pad programming access see Operations Manual Won a pIAGWOSTCL P ad F EccO airals Lers a MITBOPBOCESSOR C CT ROL ST3TER Sentinel Gold Microprocessor Control System Programmable When programmed ON the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restricts unauthorized adjustments e an airflow
4. LB2 6B_ 1 8 meters 6 2030 x 815 x 1610 mm 79 9 x 32 0 x 63 3 WxDxH With Optional Base Stand 1115x815 x2321 mm 711mm 28 type 43 9 x 32 0 x 91 4 1420 x 815 x 2321 mm 55 9 x 32 0 x 91 4 1725 x 815 x 2321 mm 67 9 x 32 0 x91 4 2030 x 815 x 2321 mm 79 9 x 32 0 x 91 4 Internal Dimensions Wx D x H 970 x 623 x 670 mm 1270 x 623 x 6 0 mm 1 2 0 x 623 x 6 0 mm 1870 x 623 x 670 mm 38 2 x 24 5 x 26 4 50 0 x 24 5 x 26 4 61 8 x 24 5 x 26 4 73 6 x 24 5 x 26 4 Usable Work Area 0 45 m 4 8 sq ft 0 6 m 6 5 sq ft 0 75 m 8 1 sq ft 0 9 m 9 7 sq ft Tested Opening 203 mm 8 0 203 mm 8 0 203 mm 8 0 203 mm 8 0 Average Inflow 0 53 m s 105 fpm LOVAG Downtlow 0 31 m s 60 fpm Inflow 376 m7 h 223 cfm 492 m h 292 cfm 608 m h 361 cfm 724 m3 h 429 cfm Downflow 628 m h 363 cfm 822 m h 476 cfm 1016 m h 588 cfm 1210 m h 700 cfm Concurrent Balance Value CBV Exhaust Volume at corresponding Static Pressure 1127 m h 658 cfm is for HVAC sizing eters Note Must use this for C sizing 1476 m h 862 cfm 1853 m h 1091 cfm 2173 m h 1269 cfm Minimum exhaust static pressure for clean exhaust filter 400 Pa 1 6 in H O 375 Pa 1 5 in H 0 375 Pa 1 5 in H 0 400 Pa 1 6 in H 0 CBV Static Pressure with additional 174 Pa 0 7 in H O required by NSF ANSI 49 2010 5 5 Pa 2
5. alarm warns of deviations from normal velocities Cabinet Construction Robust construction and enhanced safety e features qualify the cabinet for the most demanding laboratory applications The cabinet is fully assembled and ready to install and operate when shipped Consult your Esco Operating Manual or contact your Sales Representative for information on user preference programming capabilities built into the Sentinel microprocessor platform steel drain pan channel spills The drain pan is flush with the side walls to eliminate concealed or hard to clean spaces ee aid caliorarencmede males e There are no screws in the front or sides easier for certifiers to calibrate the to trap contaminants or complicate airflow display e The interior work area is formed cleaning e RS 232 data output port enables from A single piece of stainless Optional service fittings are offset for remote monitoring of cabinet operating steel with large radius 25 mm 1 easier access corners to simplify cleaning o peel ey 9 External plumbing is concealed behind e Optional TCP IP converter to connect RS The cabinet work zone has no welded 232 to network for remote monitoring joints to collect contaminants or rust dress panels to maintain aesthetics e External surfaces are coated with Esco lsocide antimicrobial coating to protect against surface contamination and inhibit bacterial growth Isocide eliminates 99 9 of surfa
6. 1 The airflow display and alarm system shall be individually calibrated before shipment 42 The main control panel shall exhibit continuous display of air velocity and a 24 hour clock display 43 Diagnostics button should be available on the control panel to easily check the cabinet operating parameters and assist servicing 44 The cabinet shall have field calibration mode that simplifies on site calibration mode 45 A selectable Quickstart mode should be available to automatically turn the blower and lights on off by moving the sash window to correct position 46 The BSC shall have RS 232 data output port for remote monitoring of cabinet operating parameters 47 TCP IP converter shall be available as an option to connect RS 232 to network for remote monitoring 48 Voltage free contacts shall be available to trigger external alarm and switch ON OFF the external blower damper 49 Pressure switch shall be used to trigger the cabinet exhaust alarm and switch the internal blower ON OFF Certification Service and Decontamination 50 The cabinet shall be approved for both hydrogen peroxide vapor HPV and formaldehyde decontamination protocol 51 All panels leading to potentially contaminated and or hazardous areas shall be color coded red 52 All components with the exception of blower motor and ULPA filters shall be located outside of contaminated air spaces to facilitate servicing without the need to decon
7. 3 iM HO Note Must use this for HVAC sizing 550 Pa 2 2 in H 0 550 Pa 2 2 in H 0 575 Pa 2 3in H 0 Supply ULPA Filter Typical Efficiency gt 99 999 for particle size between 0 1 to 0 3 microns Exhaust HEPA Filter Typical Efficiency gt 99 99 at 0 3 microns Sound NSF ANSI 49 57 58 59 60 Emission EN 12469 54 55 56 57 gt 1250 Lux gt 1400 Lux gt 1200 Lux gt 1200 Lux Fluorescent Lamp Intensity At Zero Ambient gt 116 foot candles gt 130 foot candles gt 111 foot candles gt 111 foot candles Main Body Electro galvanized steel with white oven baked epoxy polyester Isocide antimicrobial powder coated finish Cabinet Construction Work Zone Stainless Steel Type 304 with No 4B finish 220 240V AC 50Hz 10 LB2 3B1 LB2 4B1 LB2 5B1 LB2 6B 1 Electrical 110 120V AC 60Hz 10 LB2 3B2 LB2 4B2 LB2 5B2 LB2 6B2 220 240V AC 60Hz 10 LB2 3B3 LB2 4B3 LB2 5B3 LB2 6B3 Net Weight 279 kg 615 lbs 317 kg 699 lbs 359 kg 791 Ibs 438 kg 966 Ibs Shipping Weight 318 8 kg 703 Ibs 380 5 kg 839 Ibs 402 0 kg 886 Ibs 491 3 kg 1083 Ibs PE i 1210 x 940 x 1930 mm 1520 x 940 x 1930 mm 1900 x 940 x 1940 mm 2150 x 940 x 1950 mm kkkkkk Shipping Dimensions Maximum W x D x H 47 6 x 37 0 x75 9 59 8 x37 0 x75 9 74 8 x37 0 x76 4 84 7 x37 0 x76 8 Shipping Volume Maxi
8. T RP CC001 3 USA IEST RP CC007 USA IEST RP CC034 1 USA All exterior painted surfaces are powder coated with Esco Isocide an antimicrobial inhibitor to minimize contamination Isocide is integrated into the coating substrate and cannot wash out or diminish by repeated cleaning Performance results are available upon request Labculture Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets Electrical Safety UL 61010 1 USA CAN CSA C22 2 No 61010 1 EN 61010 1 Europe IEC 61010 1 Worldwide e Each cabinet is shipped with a comprehensive user s manual complete with a report documenting all test procedures e Additional IQ OQ documentation is available upon request e Contact your local Sales Representative for specific warranty details or docu mentation requests Accessories and Options Esco offers a variety of options and accessories to meet local applications Contact Esco or your local Sales Represen tative for ordering information Support Stands e Fixed height available 711 mm 28 or 864 mm 34 With leveling feet 38 1 mm 1 5 With casters e Telescoping height stand for leveling feet nominal range 660 mm or 960 mm 26 or 37 8 e Telescoping height stand for casters nominal range 660 mm or 880 mm 26 or 34 6 Adjustable in 25 4 mm 1 increments e Cradle stand electrical hydraulic infinitely adjustable with casters Elevates to s
9. and easy to clean without crevices or joints The lower drain trough is a single piece fabrication with wide open angles and a channel to direct spills to the drain The closed sidewall contains no perforations air return slots or other hidden areas where contaminants can accumulate WORLD CLASS WORLDWIDE Biosafety Cabinets Standards Compliance Dynamic Chamber Plenum Design E High negative pressure I Negative pressure I Positive pressure The Esco triple wall design creates a Dynamic Chamber plenum which surrounds contaminated areas with negative pressure preventing the possibility of contamination from leaks in filter seal gasket or cabinet structure The third wall conceals utilities Comfortable Ergonomic Design The LB2 cabinet is engineered for comfort utility value and safety e The 10 angled viewing window and narrow profile front grille improves reach into the work area e The instant start 5000k fluorescent lamp operates on an electronic ballast to reduce heat improve comfort and conserve energy e The lamp delivers uniform lighting to the work surface for greater comfort reduced glare and improved productivity see Technical Specifications e The front armrest is raised above the workzone to improve comfort and to ensure that the operator s arms U NSF ANSI 49 USA LB2 4B2 LB2 6B2 SFDA YY 0569 China Air Quality ISO 14644 1 Class 3 Worldwide JIS B9920 Clas
10. arget agar plates are placed throughout the entire work surface e Acceptance The number of Bacillus Subtilis CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet protection from cross contamination of samples placed simultaneously inside the workzone e A nebulizer containing 55 mL of spores 5 to 8 x 10 mL is placed against one of the workzone sidewalls e Target agar plates are placed 36 cm 14 inches away from the same side wall as the nebulizer e Acceptance The number of Bacillus Subtilis CFU recovered on agar plates shall not exceed 2 CFU per test HPV Test Compliant Safer Hydrogen Peroxide Vapor Decontamination Compatibility Esco biological safety cabinets are Hydrogen Peroxide Vapor HPV compliant and decontaminatable cabinets tested with both BIOQUELL and STERIS patented processes HPV is asafer and more efficient alternative to conventional decontamination using formaldehyde CH 0 e HPV is non carcinogenic and odorless while formaldehyde is carcinogenic toxic and has pungent smell e HPV biological efficacy is independent of environmental variables whereas formaldehyde efficacy is dependent on such variables e f there is a gap on the cabinet sealing escaping HPV to the lab will decompose to become oxygen and water Escaping formaldehyde however is harmful to people in the lab Therefore HPV decontamina
11. by Kl Discus test European Standard EN12469 2000 on statistical sampling basis to validate operator personnel protection The retention effi ciency for the front aperture shall be not less than 99 999 Microbiological testing for cabinet performance shall also be performed on a statistical sampling basis Filtration System 6 The cabinet shall have one supply downflow filter and one exhaust filter Supply filter shall be ULPA type per IEST RP CC001 3 and meet EN1822 H14 requirements Exhaust filter shall be HEPA type per IEST RP CC001 3 and meet EN 1822 H13 requirements 7 The filters shall be within an aluminum frame with mini Labculture pleat design without aluminum separators no wood or fiberboard shall be used in the filter assembly 8 Typical ULPA filter efficiency shall be gt 99 999 for 0 1 to 0 3 microns Typical HEPA Filter shall be gt 99 99 at 0 3 microns 9 An integral filter guard shall be affixed to prevent damage to the filter media 10 The filters shall be a individually scan tested by the manufacturer b individually scan tested after assembly and b easily accessible for scan testing in situ by means of a dedicated upstream sampling port accessible from within the cabinet 11 The supply filter shall be angled and oriented to the 10 cabinet front angle to maximize downflow uniformity over the work surface 12 A removable perforated metal diffuser shall be installed below the supply
12. ce bacteria within 24 hours of exposure e All stainless steel work surfaces are RS 232 SERIAL removable for cleaning NTERFACE PORT a e Tray components lift and remove to provide easy access and encourage surface decontamination e A recessed central area and stainless Robust Cabinet Construction and Enhanced Safety Features Service fixtures are offset for easier reach Standard cabinets include two fixture provisions on each sidewall one provision on each sidewall for 0 9 meter 3 ft cabinet Electrical outlets are mounted below service fixtures to minimize obstructions Helpful for certifiers the hinged maintenance assembly opens to a fixed position on integrated gas spring struts providing front service access All key components with the exception of the blower motor assembly are mounted outside the air stream and away from contaminated air to permit service without decontamination These include fluorescent lamps UV lamp electrical harnesses electronic boards and microprocessor control Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical shock are color coded to warn service technician The telescoping Dynamic Chamber plenum minimizes physical lifting and accelerates filter change when required Work area containment is maintained even when removable components are lifted out for cleaning The one piece stainless steel work tray edges are radiused
13. drain 22 The closed sidewall shall be sealed without perforations return air slots or concealed areas which can contain contaminants 23 The cabinet shall be free of sharp edges non functional protrusions bolts screws or hardware and all metal edges shall be deburred Ergonomics and Convenience 24 The front sash shall be frameless to maximize visibility and accessible for cleaning front and back Sash glass shall be safety glass 25 The sash counterbalance shall be suspended on two high strength cables and the sash shall lock into position in the event one cable becomes detached 26 Magnetic not mechanical proximity sensors shall work in conjunction with the control system to indicate proper sash position for containment 27 Fluorescent lamps shall be mounted behind the control panel module out of the work zone Electronic ballasts shall be used to eliminate flicker extend lamp life and reduce heat output 28 The UV lamp shall operate via an automatic timer with automatic shut off managed by the microprocessor controller and shall be interlocked with the blower motor and fluorescent lights for safety 29 The cabinet shall be designed with a 10 angled front to optimize user comfort reduce glare and maximize reach into the work area 30 The front grille shall be raised to prevent airflow blockage and improve comfort Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabi
14. eating or standing work surface height When lowered permits movement through standard doorway Note Increases exterior dimensions e Requires flexible height duct connection The sash window can be lowered further down by removing the arm rest to easily clean the upper back side of the glass Model LB2 Biological Safety Cabinet Technical Specifications 295 mm 11 6 LB2 3 406 mm 15 9 LB2 4 643 mm 25 3 LB2 5 820 mm 32 2 LB2 6 ws asa 1 Exhaust collar 2 Exhaust HEPA filter 3 Blower 4 Downflow ULPA filter angled z z sidl Ao Be 5 Downflow Sensor HARA HAZA 6 UV light Retrofit Kit provision 7 Single piece stainless steel work tray 8 Electrical Electronic panel 9 Fluorescent lamp 10 IV Bar Retrofit Kit provision SUS SS HH o 11 Service Fixture Retrofit Kit provisions 2 on Each Side 12 Electrical outlet Retrofit Kit provision
15. ers 0 15 0 20 0 25 Particle Size um 0 30 0 40 provide 99 999 typical efficiency for particle sizes of 0 1 to 0 3 micron level ESO WORLD CLASS WORLDWIDE 0 50 ULPA filtered air _ Unfiltered potentially contaminated air _ Room air Inflow air for a 50 increase in pressure loss over clean filters without manual speed control adjustment The permanently lubricated direct drive external rotor motor blower reduces operating costs The external rotor motor design allows for optimum cooling of the motor during extended operations and extends the motor bearing life Built in RFI and electrical noise filters eliminate interference with adjacent instrumentation An integral blower hour meter tracks operating life and aids in predictive maintenance planning To prevent fan damage a paper catch grille traps papers or towels that may drop down on the drain pan preventing Labculture Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets Side capture zones Dynamic air barrier inflow and forward directed downflow air converge Ambient air is pulled through the front grille to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Ambient air is taken in through a pre filter at the top of the cabinet and passes thro
16. filter to optimize airflow uniformity and to protect from damage Blower System 13 The cabinet shall have a direct drive permanently lubricated centrifugal blower motor dynamically balanced in two planes compliant to ISO 2710 for low noise low vibration and long filter life 14 The blower motor shall have an external rotor design and include an automatic thermal cut out to disable the motor in case of overheating 15 The blower motor shall compensate for filter loading 16 The blower motor system shall be enclosed within a dynamic chamber shaped steel plenum and integrated with the removable supply filter assembly to simplify filter changing 17 The cabinet shall be equipped with an integral exhaust collar for connection to the laboratory ducting system Cabinet Design Construction Cleaning 18 The cabinet shall be of triple wall design whereby all positive pressure plenums capable of handling contaminated air shall be surrounded by negative pressure No positive pressure areas shall be accessible external to the cabinet The third wall shall conceal utilities 19 The cabinet shall maintain containment performance even when removable work area components are removed for cleaning 20 The work tray shall be one piece removable stainless steel with radius corners without crevices or joints 21 The cabinet shall have a stainless steel one piece fabricated drain trough with open angles to channel spills to acommon
17. front air grille to establish operator protection room air does not enter the work zone preventing product contamination The front grille has proportionally larger perforation on the extreme left and right side Combined with the side air gap between the work tray and the side wall the stronger side capture zones in crease protection in this critical area The inflow grille has a convex shape to maintain operator protection by discouraging users from placing objects on it Raised armrest prevents the likelihood of the inflow grille being blocked by the operator s arms Auto purge openings located at the front side walls eliminate eddy currents and dead air pockets in the critical area behind the sash window The ULPA downflow supply filter is positioned proportionally to the cabinet front angle to direct more air forward to the front air grille The inflow velocity downflow velocity air flow path and intake geometry are precision tuned and tested to create an optimum air curtain on the front opening this curtain maintains personal and product protection by increasing barrier protection Integrated Filtration System A combination of a supply ULPA filter and an exhaust HEPA filter give the Labculture cabinet a fully integrated performance envelope for product operator and environmental protection ULPA supply filter per IEST RP CC001 3 is tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micro
18. g the KI Discus method for operator safety Purchase Specifications LB2 Series Class Il Type B2 Total Exhaust Biological Safety Cabinet General Performance and Certifications 1 The biological safety cabinet shall comply with one or more of the following international standards and the manufacturer shall provide a certified copy of containment and performance tests equivalent to or greater than specified in the following independent international standards for biological safety electrical and other functional characteristics Class Il Type B2 per NSF ANSI 49 USA and SFDA YY 0569 2 The cabinet shall protect a the operator and laboratory environment from particulates generated within the work zone b the product and process within the work zone from airborne contamination from ambient air c and the product and process within the work zone from cross contamination 3 Each cabinet shall be listed by Underwriters Laboratories UL cUL or CE for electrical safety 4 Original documentation specific to each cabinet serial number shall be provided with the cabinet and maintained in the manufacturers records Test data verifying all perfor mance criteria shall be available upon request to include a inflow velocity through direct inflow measurement method b downflow velocity and uniformity c filter leak scan with aerosol challenge for both filters d electrical safety 5 The cabinet shall be tested
19. haust collar for connecting the cabinet exhaust to an airtight ducting system NB Ductworks are not provided with standard product Front Sash Assembly Integrated sash proximity contacts sense sash position serve as an interlock for the UV lamp and activate an alarm if the sash is improperly positioned The magnetic switch eliminates the chance of mechanical wear and tear typical of a mechanical switch The back of the sash can be easily cleaned by removing the arm rest and lower the sash glass The sash is counterbalanced for smooth and light sash moving operation The counterbalance locking mechanism is inherently safe it locks the counter balance in place if either of the 2 cables is detached The sash cable and cable clio have rated strength of more than 6x the weight of the sash window of the largest Labculture Class II cabinet 1 8 meter 6 ft model The safety glass maintains containment if the sash is accidentally broken during cabinet operation Blower Efficiency The Labculture blower system is de signed for high performance operation maximum energy efficiency and minimal maintenance The blower motor can maintain airflow velocity within 10 of original setpoint Esco cabinets use supply ULPA filters per IEST RP CC001 3 instead of conventional HEPA filters commonly found in biological safety cabinets While HEPA filters offer 99 99 typical efficiency at 0 3 micron level ULPA filt
20. mum 2 19 m 77 3 cu ft 2 76 m 97 4 cu ft 3 46 m 122 2 cu ft 3 94 m 139 1 cu ft z height includes exhaust collar FE This Concurrent Balance Value CBV Exhaust Volume per Pitot Duct Traverse and Static Pressure at cabinet exhaust connection should be used when sizing the HVAC exhaust amp supply This minimum exhaust static pressure for clean exhaust filter should not be used for exhaust fan sizing and it is listed here for comparative purpose only Noise reading at open field condition anechoic chamber Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand Comprehensive Performance Testing At Esco Labculture Inflow downflow velocity Airflow pattern visualization Electrical safety to EC61010 1 Additional KI Discus containment and microbiological Every Labculture LA2 model manufactured by Esco is individually tested documented by serial number and validated with the following test methods PAO aerosol challenge for filter integrity testing are performed on statistical sampling basis Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets Microbiological Testing Esco performs testing in accordance with more than 10 of the world s most recognized standards for local regional and international criteria Testing in our microbiology laboratory is conducted according t
21. n particles providing better filtration capability than conventional HEPA filters that have a typical efficiency of gt 99 99 for 0 3 micron particles HEPA exhaust filter per IEST RP CC001 3 is tested to a typical efficiency of gt 99 99 for 0 3 micron particles Modern separator less mini pleat filter construction maximizes the filter surface area to extend filter life and eliminate possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction The filter frame and media is constructed in accordance with EN1822 requirements for fire retardant properties Mini pleat Separatorless Filter left vs Conventional Aluminium Separator Filter right Esco cabinets use Camfil Farr mini pleat filters without aluminum separators to increase filter efficiency minimize the chance of leakage and to prolong filter life Filters include a lightweight aluminum frame for structural stability and elimination of swelling common to conventional wood frames Esco ULPA Filter Efficiency Typical Penetration 0 0002 A E The supply ULPA filter provides ISO Class 3 per IS014644 1 clean air to the work surface in a gentle vertical laminar flow for product protection The exhaust HEPA filter traps biohazard particles acquired from the work surface before air is exhausted via the ducting system to the external environment The Model LB2 is provided with an integral ex
22. nd other printed materials Esco reserves the right to alter its products and specifications without notice All trademarks and logotypes in this material are the property of Esco and the respective companies 9010009_Biolo Tee PT Esco Bit Cert Ni intan Indonesia lo 651333 E
23. nets 31 Penetrations for petcocks and service fittings shall be provided penetrations shall be offset to improve user access 32 The cabinet shall accommodate an optional mounting stand for fixed height Control and Alarm System 33 All cabinet functions shall be managed by a programmable microprocessor control system capable of software updates via Internet downloads 34 The microprocessor controller shall be mounted on the main control panel facing down toward the user 35 The controller shall include soft touch keypad controls and backlit LCD displays to permit operation of the blower motor light UV lamp electrical outlet s and menu 36 The controller shall be user programmable in situ to enable or disable functions such as PIN personal identification number access restriction cabinet start up protocol airflow alarm and other microprocessor controlled operations outlined in the user manual 37 When programmed ON the start up protocol shall perform an automatic pre purge and post purge cycle to ensure proper cabinet operation 38 The controller shall include a blower motor hours meter to display aggregate motor running time to assist in predictive maintenance 39 Audible and visual alarms shall be provided for unsafe conditions such as improper airflow or sash position 40 Airflow shall be monitored by a temperature compensating thermistor based true air velocity sensor mounted in the cabinet 4
24. o NSF ANSI 49 EN 12469 and JIS K3800 An NSF accredited biohazard cabinet field certifier is available in house full time to supervise all tests Harmless Bacillus Subtilis bacteria is used to challenge the cabinet then incubated for 48 hours and the Colony Forming Units CFU are counted to determine the testing results Increased microbiological challenge tests with objects inside the cabinet work zone bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel Protection Test The test objective is to evaluate the safety of the cabinet for the personnel operating on potentially hazardous samples in the cabinet workzone e A nebulizer containing 55 mL of 5 to 8 x 108 spores mL B Subtilis spores is placed inside the workzone 10 cm 4 inches behind the front opening sash e Target slit air samplers and impingers are placed outside the workzone to capture B Subtilis spores that may escape then the sample is incubated e Acceptance The number of B Subtilis CFU recovered from the agar plates shall not exceed 10 CFU per test Product Protection Test The test objective is to determine cabinet protection to the product samples inside the cabinet workzone from environmental contaminants e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B Subtilis is placed at 10 cm 4 inches in front of sash window e T
25. ofile The completely integrated assembly optimizes motor cooling All rotating parts are unitized and balanced for smooth quiet vibration free operation A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone The downflow combined with the inflow air enters the common air plenum All air in the common plenum is HEPA filtered and exhausted via a dedicated ducting system to the external environment An integrated temperature compen sated true airflow velocity sensor provides an accurate airflow reading despite room temperature fluctuation All electronic parts are contained inside a plug and play module Microprocessor software updates are available from Esco for download Sentinel functions are factory set to default to ON or OFF depending on worldwide destination and local preferences Default settings can be user activated through the touchpad data entry access The airflow alarm can be activated or deactivated depending on user preference and nature of the work Automatic start up sequence will prepare the cabinet for normal operation and advise when safe conditions are established Selectable Quickstart mode to automatically turn on off the cabinet blower and lights by simply moving the sash window to the correct position An administrator controlled PIN
26. rt Removable one piece work surface simplifies cleaning Raised airflow grille maintains safety by preventing blockage Laa tal i Esco ISS lt i E antimicrobial coating on all painted surfaces minimizes contamination Negative pressure plenum surrounds supply positive pressure plenum no fabric bags are The cabinets are Kl Discus tested on sampling basis for performance integrity SFDA u Available in 0 9 1 2 1 5 and 1 8 meter models 3 4 5 and 6 Shown with optional telescoping stand Esco Labculture has passed more performance tests in more languages for more certifications throughout more countries than any other biological safety cabinet in the world Labculture Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets rece Operator Product and Environmental Protection The Esco Labculture Class II Type B2 Total Exhaust Biological Safety Cabinet provides operator product and environmental protection for biosafety Levels 1 2 and 3 This cabinet can be used for handling Biohazard Level 4 provided that the operator wears positive pressure sult Containment and Protection The total exhaust design of the LB2 makes the cabinet suitable for microbiological work involving trace amounts of toxic chemicals The non recirculating design guarantees Safety in cases where exhaust HEPA filtration fails to ensure containment Inflow of room air enters the
27. s 3 Japan JIS BS5295 Class 3 Japan US Fed Std 209E Class 1 USA do not block the forward airflow perforations e The optional adjustable support stand provides work surface height control e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone provides ample room for transferring of small equipment See Specifications e The sliding window can be fully opened Max sash opening is 457 mm 18 to insert and remove larger instrumentation and equipment Electrical Safety and Certification All components meet or exceed applicable safety requirements e Each cabinet is individually factory tested for electrical safety e Documentation specific to each cabinet serial number is maintained on file e UL Listed for USA and Canada e Certified to the US standard NSF ANSI 49 for Class Il biohazard cabinetry refer to Standard Compliance table at the top of this page for the full list e Contact Esco or your Sales Representative for site preparation information see Electrical Specifications Warranty The Labculture cabinet is warranted for 3 years excluding consumable parts and accessories 1E Antimicrobial Powder Coating OV D Bacterial Population 10 folds N 12 hours 8 hours 0 4 hours 16 hours 20 hours 24 hours Contact Time in Hours Filtration EN 1822 H14 amp H13 Europe IES
28. taminate the cabinet 53 All exterior surfaces shall be painted with a permanent antimicrobial inhibitor coating to minimize contamination Installation for Labculture Class II Type B2 Total Exhaust Biological Safety Cabinet Check List 7 Ensure a Class II Type B2 cabinet is 2 Exhaust Blower at Terminal End Preferably individual and Connected to Emergency Power Pitot Tube Duct Traverse Penetration Drill Penetrations in Ductwork Anti Blowback Valve Optional Air Tight Damper Required Enables static pressure to be adjusted as filter loads to maintain airflow volume Y 4 lt q Enables the cabinet to be sealed off during decontamination aS isoce Labculture Note Cabinet shown with optional support stand optional air tight damper optional anti blowback valve and optional exhaust blower Exhaust ductwork is not provided by Esco Supply Air Make Up for Downflow and Inflow Air Located far away from exhaust blower loss for the specified airflow volume in 5 Provide an air tight damper the ductwork mandatory and anti blowback valve 3 A dedicated exhaust system one optional exhaust blower to one cabinet is 6 Provide provisions for pitot tube duct strongly recommended It is also traverse These can be drilled in the recommended the exhaust system ductwork on site be operated continuo
29. tic UV lamp timer through Sentinel micro processor control panel Emission of 253 7 nanometers for most efficient decontamination Lamp is positioned away from operator line of sight for safety and proper exposure to interior surfaces Note UV lamp intensity reduces over time and its effectiveness is subject to factors such air temperature and microbial species in the work zone e PVC armrest Chemically treated improves s operator comfort easy to clean 711 mm 28 standard size e Ergonomic lab chair Laboratory grade construction meets Class 100 cleanliness alcohol resistant PVC materials Adjustable 395 490 mm 15 6 19 3 Stainless steel construction Available for all standard Esco cabinets Microscope viewing device Mounting and viewing pouch integrated into sash Factory installed specify when ordering Anti blowback valve Prevents flow from the facility HVAC system into the Esco product e Air tight damper Seals the Esco product from the e Ergonomic foot rest Angled helps maintain proper posture Adjustable height Anti skid coating chemical resistant finish facility HVAC system during decontamination ESCO WORLD CLASS WORLDWIDE 1115x815 x 1610 mm Without Base Stand 43 9 x 32 0 x63 3 External Dimension 1420 x 815 x 1610 mm 55 9 X 320 X 63 3 General Specifications LB2 3B_ LB2 4B_ LB2 5B_ 1725 x815 x 1610 mm 67 9 X S201 XGI
30. tion can be performed while people still working inside the lab while formaldehyde decontamination must be performed with no one present in the lab The HPV method improves safety productivity and reduces the time to seal the cabinet HPV has a better penetration capacity resulting in a full decontamination of the cabinet The formaldehyde method is known to result in incomplete decontamination HPV is more effective and rapid against biological organisms compared to formaldehyde HPV requires approximately 4 7 hours for set up decontamination and tear down compared to a total of 12 15 hours needed to complete a formaldehyde decontamination process HPV decontamination effectiveness is independent of temperature and humidity Formaldehyde requires temperature above 20 C and relative humidity above 65 For information on the BIOQUELL and STERIS HPV methodologies contact Esco or your Sales Representative and ask for our HPV Decontamination Whitepapers 9 The Performance Envelope Concept High Int Enn i High Desanntiow Male High inflow 1S8 Lowy Downfkas 026 i Nominal Inflows i 5a Nominal Dovenflave 0 21 LOAF imil th i cna infi Cow Downie 026 0 25 0 20 035 0 40 045 Lovania webod ly High Dormia 0 40 0 mi s Esco cabinets are designed to operate within a performance envelope to maintain protection for personnel product and the environment Airflo
31. ugh the downflow ULPA filter entering the work zone as laminar flow The uniform non turbulent air stream protects against cross contamination within and throughout the work area Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small blue arrows them from being pulled into the column by fan suction Sentinel Microprocessor Control Alarm Monitoring System The Esco Sentinel microprocessor based control system supervises operation of all cabinet functions e The control panel is located on the center of the cabinet and angled down for easy access by the operator e Continuous monitoring of cabinet airflow is displayed on a bright easy to read LCD panel e A temperature independent fast response pressure switch is used to trigger the airflow alarm and turn off the internal blower should exhaust is insufficient Esco Centrifugal Fan with External Rotor Motor left vs Conventional Fan with Standard Motor right Esco cabinets use German made ebm papst permanently lubricated centrifugal motor blowers with external rotor designs ntegrated blades norrow the profile and eliminate the need for a motor shaft Motors are selected for energy efficiency compact design and flat pr
32. usly 7 Observe Esco recommendations on 4 Ensure the lab is supplied with suitable cabinet location especially sufficient makeup air required for the application Provide an exhaust system ductwork and blower capable of delivering the required airflow volume and static pressure at the connection point note the Required CBV Exhaust Volume and NSF required Static Pressure listed on Page 8 Remember to add the pressure putting the cabinet away from external airflow disturbances 8 Certify cabinet after installation before use ESCO WORLD CLASS WORLDWIDE 11 2 escoglobal corr LIVE SUPPORT qi C Biological Safety Products Cleanroom Products Containment Pharma Products Ductless Fume Hoods Carbon Filtration General Purpose Scientific Equipment Industrial Lab Equipment In Vitro Fertilization Products PCR Products Pharmacy Products Lab Animal Research Products Lab Thermostatics Products Lab Ventilation Chemical Fume Products Lab Furniture Powder Handling Products Since 1978 Esco has emerged as a leader in the development of controlled environment laboratory and cleanroom equipment solutions Products sold in more than 100 countries include biological safety cabinets cleanroom products compounding pharmacy equipment containment pharma products ductless fume hoods in vitro fertilization workstations lab animal research products laboratory fume hoods laboratory o
33. vens and incubators laminar flow clean benches and PCR products and instrumentation With the most extensive product line in the industry Esco has passed more tests in more languages for more certifications throughout more countries than any biosafety cabinet manufacturer in the world Esco remains dedicated to delivering innovative solutions for the clinical life science research and industrial laboratory community www escoglobal com Biological Safety Cabinets and Laminar Flow e Laboratory Fume Hoods e Laboratory Ovens Laboratory Incubators e PCR Thermal Cyclers e Microplate Shaker Incubators e Ultralow Freezers ESCO WORLD CLASS WORLDWIDE Esco Technologies Inc e 2940 Turnpike Drive Units 15 16 e Hatboro PA 19040 USA Toll Free USA and Canada 877 479 3726 Tel 215 441 9661 Fax 215 441 9660 us escoglobal com usa escoglobal com Esco Micro Pte Ltd e 21 Changi South Street 1 e Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 mail escoglobal com www escoglobal com Esco Global Offices Beijing China Kuala Lumpur Malaysia Manama Bahrain Guangzhou China Hanoi Vietnam Marietta OH USA Melaka Malaysia Mumbai India Philadelphia PA USA Salisbury UK Shanghai China Seoul Korea Delhi India Singapore p Esco Micro Pte Ltd Cert No 651076 gical Safety Cabinet_LB2_Brochure_A4_vA1_08 11 Esco can accept no responsibility for possible errors in catalogues brochures a
34. w parameters used to frame the performance envelope include both inflow velocity and downflow velocity The graph illustrates the boundaries of the performance envelope as well as the nominal performance point at which tests are conducted The range between high and low Inflow and high and low downflow together with the fluid dynamics achieved through sophisticated cabinet design proportionally size capture slots and uniform laminar airflow combine to deliver a complete containment and safety solution expected of a professional biological safety cabinet For details on the Esco performance envelope contact Esco or your Esco Sales Representative ESCO WORLD CLASS WORLDWIDE 10 KI Discus Containment Test According to EN 12469 Potassium lodide Esco is currently one of the few companies in the world equipped to perform the KI Discus test for our customers The KI Discus test is defined in the European Standard for microbiological safety cabinets EN 12469 as a test method for validating the operator personnel protection capabilities of the cabinet e The KI Discus test shows excellent correlation with the microbiological test method for operator protection and is useful for validating the actual containment performance of the cabinet on site e The Kl Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Thus Esco Labculture LB2 models are factory tested on a sampling basis usin
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