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PhotonTM

1. Abnova Corporation OF No 108 Jhouzih St Neihu Taipei 114 Taiwan Te 886 2 8751 1888 Fax 886 2 8751 1166 version 1 0
2. MG Abnova USER MANUAL Photon 96 well Chemiluminescence System Photon ey Abnova www abnova com CG Abnova Table of Contents Safety Items 2 Chapter 1 Photon Overview 3 Chapter 2 Installation 4 Chapter 3 USB Driver Installation 8 Chapter 4 Software Settings 13 Chapter 5 Photon Operations 16 Using the Default Settings 16 Il Save File in CSV for MAT 17 Ill Kinetics or Temporal Studies 18 www abnova com 1 Photon Safety Items A Caution 1 The Photon includes the system manual CD power cord and a serial cable Photon is a very delicate system with optoelectronics therefore please handle with extreme care 2 To prevent electrically related injuries and to identify any property damage during shipment properly inspect all electrical equipment priors to use and immediately report any damage or defects Contact an Abnova Sales Representative for any servicing of the equipment that requires removal of covers on system 3 To avoid electric shock do not open the system use the supplied power cords only and connect to properly grounded wall outlets 4 Do not expose the system to strong light Direct sunlight and other strong illumination may cause damage of the photon detector even when the system is not in operation We recommended that you close the microplate cover immediately after placing the sample plate into the system Do not open the cover when the system is ru
3. Ready to Install Setup is now ready to begin installing Photon on your computer Click Install to continue with the installation or click Back if you want to review or change any settings Destination location C Program Files Photon Start Menu folder Photon 9 Click on Finish when prompted j Setup Completing the Photon Setup Wizard Setup has finished installing Photon on your computer The application may be launched by selecting the installed icons Click Finish to exit Setup 10 Connect the power cord and plug to a grounded wall outlet Photon operates at 100VAC 240VAC and 50Hz 60Hz Abnova A Connect the USB or B Connect the serial cable 11 Plus cable into the host PC A USB connection see the next B Serial connection go section USB Driver Installation to Software Operation for more information 12 Turn the power on www abnova com Photon USB Driver Installation USB connectivity is under testing and is subject to change If you use serial cable please skip this section and go to next section When the first time you attach USB cable to a PC it will prompt you to install the driver 1 Check Install from a specific location Advanced and click on Next Hardware Update Wizard Welcome to the Hardware Update Wizard This wizard helps you install software for Photon USB cable COM5 If your hardware came with an installat
4. 1 10 seconds per well and any number of scans according to your choice The system can also be used for kinetic and luminescence time profile studies After clicking on the Go bottom the system will automatically and quickly scan all of the reactions in the microwells and display the results Photon uses a Windows based PC or notebook computer as its microprocessor The system is interfaced to the computer by a simple plug in serial port connection A CD with user friendly software is provided for easy installation The resulting data can be displayed as a spreadsheet in Microsoft Excel or exported as a comma delimited CSV file The data reported as the number of optical counts is displayed as it is collected The software also includes utilities for routine calibration and operation The resulting data can be saved or printed out The system approximately 16 x 11 x 4 and 20 Ibs is easily portable for sharing between laboratories or researchers www abnova com Photon Installation 1 Please read Safety Information before proceeding Carefully unpack Photon fromthe box The package includes the Photon system manual CD a power cord and a serial cable Photon is a very delicate system with optoelectronics therefore please handle with extreme care 2 Insert CD in the host PC Window 2000 XP with Excel 3 Use Windows Explorer to browse the CD Double click Setup exe to begin the i
5. ERPETEPEEEPE BA H H H H Gg Integration Time ms 100 Experiment HRP 3 ZH 8 S Ss Select All 4 Unselect All 3 Go 4 ica ce AE EENET 4 8 The next time you want to do the same temporal study you only need to click on the Temporal project button and select the single well you want to read Temporal Empty Empty Empty Default se Ill Kinetics or Temporal Studies of multiple well 1 Power up the Photon 2 Fill the wells on the microplate with reagent 3 Place the microplate on the Photon Make sure the Al well is on up right corner Abnova 4 Launch Photon software and click on the Setting tab Photon Selection Setting Data Delay between ZONE Delay between fi pass sec well ms Number Of Pass 10 Com Port USB gt Output File Dir CA Photon 1 Browse Empty Empty Empty Empty Default Save Proj 5 Change Delay between pass to 60 seconds and change the number of passes to 10 6 Click on Selection tab again Select the wells you want to do temporal study on and click Go Photon Uma Experiment ey 4 da H EH Integration Time ms da a Ia a r Select All jy TE Unselect All Go lt ERRER 7 Effectively this setting will read A6 B6 C6 to H7 than delay for 60 second And go back to read A6 B6 C6 to H7 again It will repeat for 10 times www abnova com Photon
6. There are 4 project spaces you can use Project Name try1 Project 1 X OK i Project 1 button If you have saved the settings for a project the name of the project will display on the project button By clicking on this button you can retrieve the setting you have saved try1 Empty Empty Empty Default Save Proj j Project 2 button The same as above k Project 3 button The same as above Project 4 button The same as above 3 The Data tab The data and reading will display on this screen Photon Selection Setting Data Running ITOTMOONOO Do Not Open Cover a Stop You may stop Photon while it is still running b Scroll Bar Due to the limited screen display size Photon could not display all of the data in one screen You may drag the scroll bar to scroll the data view www abnova com Photon 4 How Photon naming the data files and where Photon saves the data file The system will save the data file using the file name as YYYY_MM_DD_Experiment_ hh_mm_ss xl s YYYY stands for year in 4 digits MM stands for month DD stands for date hh stands for hour mm stands for minute and ss stands for second Experiment is the same text as you type in previously If you did not type anything in the Experiment field the file name will be YYYY MM DD hh mm ss xls The system will save the file to the location of Output file Dir Operator The default of Outp
7. age box Click OK to dismiss the dialog and the system will automatically launch Excel spread sheet 10 When you next launch the Photon software all of the settings will be the same as from the previous session therefore you only need to select the well you want to read and click the Go button Il Kinetics or Temporal Studies of single well 1 Power up the Photon 2 Fill only one well on the microplate with reagent 3 Place the microplate on the Photon Make sure the Al well is on up right corner 4 Launch Photon software and click on the Setting tab Selection Setting Data Delay between bo oo Delay between fi pass sec well ms Number Of Pass ho Com Port usb Output File Dir ca Photon Browse Empty Empty Empty Empty Default Save Proj www abnova com Photon 5 Change Delay between pass to 60 seconds and change the number of passes to 10 Effectively this setting will read the well 10 times at a 60 second delay between reads Make sure you select Use file name and type the file name next to check box 6 Click the Save Project button and type Temporal in the project name field Click on OK to dismiss the dialog Project Name Tempora Project 1 M OK 7 Click on Selection tab again Select the single well you want to do temporal study on and click Go This will take more than 10 minutes 60 sec x 10 passes 10 minutes Selection Setting Data J
8. are from the program icon on the desktop or the Start menu The main Photon window is shown below J H b Photon COX Operator a A c Experiment d Integration Time ms 100 e Select All f Unselect All g A There are three tabs Selection Setting and Data in the main program window Each tab button is responsible for specific information and tasks related to Photon operation Click on each tab button to change the display 1 The Selection default tab shown above is automatically selected upon launching the Photon application The boxes in the window allow the user to select one some or all of the wells in the micoplate a There are eight row buttons from A to H Click on A B C etc to choose the all wells in that row For an individual well selection if the well is not checked click on the well to select and click it again to unselect b There are 12 column buttons Click on 1 to 12 to select the all wells in that column c The Operator field You may type your name in this field The text in this field will appear in the spread sheet It will also effect where the data file save to Please refer to IV How Photon naming the data file and where Photon save the data file d The Experiment field You may type a short description in this field The text in this field will transfer to the data file e Integration Time ms Set the integration time in millisec
9. g signal in some wells we Suggest you increase this delay for better performance d Com Port Select the com port that Photon is attached to This combo box will only list the com ports your system has If the com port that Photon is attached to does not show in the combo box please refer to the Q amp A section e Output file Dir It will display the location of the data file Please refer to IV How Photon naming the data file and where Photon save the data file f Browse You can click the Browse button to change which directory to which you want to save the file Clicking this button will pop up a directory selection dialog as shown here Browse for Folder P Current folder is Et Desktop a My Documents TS A Computer fh 3 5 Floppy 4 3 0 Ee Local Disk C Se Local Disk D Ee New Volume E oa DWO CD Rw Drive FE 0 E E SysShare on Server 5 a Se CommonDoc on ServersSysShare 22 au My DxDM Places di My Network Places w Cancel Y Abnova g Default You can restore all of the default settings by clicking this button h Save Project You can save the current settings so that the next time you can just click on single button to retrieve the settings Clicking on this button will display a dialog box enter the name of the project if you want to create a new project or select which existing project you want to write the settings to overriding that project s existing settings
10. ion CD or floppy disk insert it now What do you want the wizard to do O Install the software automatically Recommended Click Next to continue 2 Check Don t Search Will Choose the Driver to Install and click Next Hardware Update Wizard Please choose your search and installation options S Search for the best driver in these locations Use the check boxes below to limit or expand the default search which includes local paths and removable media The best driver found will be installed Search removable media floppy CD ROM include this location in the search CADocuments and Settings dchou Desktop Drive Choose this option to select the device driver from alist Windows does not guarantee that the driver you choose will be the best match for your hardware 2 Je Abnova 3 Select Ports COM amp LPT and Click on Next Found New Hardware Wizard Hardware Type Select a hardware type and then click Next Common hardware types TONT Apm Legacy Support A PCMCIA adapters PCMCIA and Flash memory devices A Ports COM amp LPT YA Printers SA Processors SS SBP2 IEEE 1394 Devices SCSI and RAID controllers L wa Cmar card raaders ca 4 Click on Have Disk Found New Hardware Wizard Select the device driver you want to install for this hardware Select the manufacturer and model of your hardware device and then click Next If you have a disk
11. nning 5 Read all safety instructions and understand the operation procedures before proceeding with the installation maintenance and servicing of all components Y Abnova Photon Overview Photon is a compact system designed for highly sensitive chemiluminescence and bioluminescence detection The system measures the luminescence intensity in a 96 well microplate black white or opaque These microplates have microwells with a clear bottom allowing for luminescence detection from the bottom of the well Many companies such as Nunc Costar Corning BD Biosciences Perkin Elmer etc have microplates for luminescence applications Because Photon uses a state of the art photoncounting multiplier tube as the detector element the system is extremely light sensitive Photon has the ability to detect very small amounts of analyte in the range of attomoles by counting the number of photons generated from a chemiluminescent or bioluminescent experiment The detector is sensitive in all visible ranges Photon combines the latest low noise detector and electronic circuitry to provide very low background noise and very high signal Its broad dynamic range covers over 7 orders of magnitude The superior mechanical design eliminates most of the crosstalk down to 10 between the adjacent wells The system is flexible and easy to use The user can select to scan any one or any set of the 96 wells at a measuring time of 0
12. nstallation 4 Click Next on Welcome dialog Welcome to the Photon Setup Wizard This will install Photon V 01000000 on your computer Itis recommended that you close all other applications before continuing Click Next to continue or Cancel to exit Setup 5 If you do not wish to install Photon in the default location change the path now Click Next if you want to install Photon to default location Select Destination Location Where should Photon be installed D Setup will install Photon into the following folder To continue click Next If you would like to select a different folder click Browse C Program Files Photon Atleast 2 0 MB offree disk space is required Abnova 6 If you want to change the folder name on the Start Manu change now otherwise click on Next Select Start Menu Folder Where should Setup place the program s shortcuts Setup will create the program s shortcuts in the following Start Menu folder To continue click Next If you would like to select a different folder click Browse Proton Browse 7 If you want to create an Icon on desktop check the box or click Next to continue Select Additional Tasks Which additional tasks should be performed Select the additional tasks you would like Setup to perform while installing Photon then click Next Additional icons www abnova com Photon 8 Click Install
13. odel Photon USB cable AN This driver is not digitally signed me why driver signing is important Abnova 9 The wizard will begin to install the driver Found New Hardware Wizard Please wait while the wizard installs the software J Photon USB cable D Next gt Cancel 10 Windows XP will warn that the driver has not been tested for compatibility with Windows XP Click on Continue Anyway Hardware Installation The software you are installing for this hardware Photon USB cable has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair yaa yaa destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing STOP Installation www abnova com Photon 11 Copy files Hardware Update Wizard Please wait while the wizard installs the software J Photon USB cable D D ser2pl sys To C WINDOWS System32 DRIVERS 12 Click Finish Hardware Update Wizard Completing the Hardware Update Wizard The wizard has finished installing the software for 3 Photon USB cable pa Click Finish to close the wizard Abnova Software Settings Start the Photon softw
14. onds The integration time is the time interval that the Photon will allocate for counting photons at each well 100 milliseconds is the most common used value You can type in the value The value has to be multiple of 10 f Use Select All button to select all of the wells in the selection table www abnova com Photon g Use Unselect All button to unselect all of the wells in the selection table h The Go button to start reading the microplate 2 The Setting tab controls many aspects of Photon operations Selection Setting Data Delay between i Delay between MN pass sec well ms Number Of Pass 1 Com Port COM1 M I gt g Output File Dir ea Photon 1 Browse Empty Emply Empty Empty Default Save Proj 7 i k g h a Delay between pass sec Delay time between each pass After the PMT finish all of the selected well it will delay for the time specified in this field before going to next pass b Number of Pass Select the number of passes The number of passes indicates how many times the Photon will read the microplate If the number of passes is 2 Photon will read from Al well to H12 well and than come back to well A1 to perform the second pass c Delay between well sec Delay time between readings After the PMT moves to the target well it will delay for the time specified in this field for the PMT to settle down before take a reading If you have a stron
15. that contains the driver you want to install click Have Disk 5 Click on Browse Install From Disk Insertthe manufacturer s installation disk and then make H sure thatthe correct drive is selected below Copy manufacturer s files from www abnova com Photon 6 Navigate through the Installation directory to the folder called UsbCableDriver The default directory is C Program Files Photon UsbCableDriver Select SERWPL INF and click on Open Locate File Look in UsbCableDrive gt My Recent Documents 3 Desktop My Documents 9 My Computer 3 3 5 Floppy A s Main C O Program Files Photon Ped Usb CabieD ivel amp back D amp Data E ID CD Drive F My Documents S SysShare on Server S Se CommonDoc on Server X Pr My Network Places OldFile File name SERWPLIINF My Network Files of type Setup Information inf 7 Click OK Install From Disk Insert the manufacturer s installation disk and then make sure that the correct drive is selected below Copy manufacturer s files from C Program Files Photon UsbCableD rive 8 Click on Next Found New Hardware Wizard Select the device driver you want to install for this hardware Select the manufacturer and model of your hardware device and then click Next If you have a disk that contains the driver you want to install click Have Disk M Show compatible hardware M
16. ut file Dir is C Photon For example if you type Bob in the Operator field the data file will save to C Photon Bob Photon Operations Operations using the default settings 1 Power up the Photon N Allow 30 minutes for warm up WJ Fill a 96 well microplate with reagents aN Place microplate into the Photon Make sure the Al well is in the upper right corner t is very important that you close the microplate cover immediately after placing the sample plate into the system to protect the delicate optoelectronics from exposure to outside light Open the cover only for plate placement 5 Launch the Photon software and click on the Setting tab Photon Selection Setting Data Delay between nh Delay between fi pass sec well ms Number Of Pass fi Com Port USB X Output File Dir ca Photon 1 Browse Empty Empty Empty Empty Default Save Proj 6 Select the com port that connects to Photon and leave the rest of settings at default Y Abnova 7 Click on Selection tab then click on Select All button to choose all 96 wells or click on A B C etc to choose the wells in row or click on 1 2 3 etc to choose the wells in column Selection Setting Data EAE 6 ww TES KE Es KEE E KE E 8 Click on Go button The Photon software will switch to Data display tab 9 When Photon finish reading it will pop up a Done mess

PhotonTM

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