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Multiskan FC User Manual_English

1. Settings after installation If you want to change the language or add additional filters to the filter wheel modify the required settings in the Settings menu Refer to the instructions in Settings menu Installation of Skanlt Software for Microplate Readers For installing Skanlt Software for Multiskan FC refer to the Skanlt Software for Microplate Readers User Manual Cat No N16243 Caution Operate the instrument only with software and hardware that is specifically designed or selected for it Thermo Fisher Scientific assumes no liability when third party software applications are used The instrument has one computer interface USB When the instrument is connected to Skanlt Software the Remote mode active text is shown on the display 15 The STOP buiton on the keypad can be used to terminate the computer remote control Chapter 3 Multiskan FC Main Parts Front view The front view of the Multiskan FC instrument is shown in Figure 10 Lamp and filter w chamber c Measurement cha Figure 10 Multiskan FC front view Back view The back view of the Multiskan FC instrument is shown in Figure 11 16 i Cooling outlet USB port for dili USB port for P ON OFF switch ing ou T USB port Q for memory device Esel ce S A REE s1119100 SN 257 900780r Marsfactured in Crone Thame Fisher Soarta Shanghiss ov inerumerts Co Ld Ci 5 00 1 amo Figure 11 Multiskan FC back vi
2. staal 64 go 64 Chapter 12 Emergency SIUALIONS o5eutee iere ius Deeetae E E duto bu E Pere wiu E 65 Handling emergency situations RTT 65 Chapter 13 Malle I ANG Eseia eaa iaaa aaas adaa a 65 Regular and preventative maintenance esses nennen nennen mnn nnn nennen nennen nnns 65 Maintenance CHECKS bus ies eoi poetice HE Thao etude ace Be Une dU rend Bet Vasa Ph BS aea Ph te meti d UR iu 65 EIS BIST ene NITET TTD DUE 66 EEG GIO IO cuceag cutis do rush esp tutks at tuc aote E cute Pop sa G beds VeL Pop c QR DE de M UebC Poe aebT 67 cleaning AOT sssr t ee ee 67 Cleaning Orens tamen 5225 ein ts aes aaa teeth ees eet sheesh atte ates tie tee esa hee 67 Disposal ol MUA EET T a OT 67 Decontamination procedure eessseessssesseeeeee seen nne nnne nnne nena nsu sine nena nsa sana rsen a ananas 68 Adding individual filters to the filter wheel esses nennen 69 CHANGING THC lamp MH EE 72 PICTON ThANSDON JOCK rtt D DL 74 Maint ming a System 100 pm 75 HOWTO DaACK ieu T 76 Disposal of the INStrUIM N ccccccceccceecceeeceeecauecaueceueseueceuecuesueseueseeesaeeseeeteeeseeseeeseeeseesseeeseesseeess 76 Chapter 14 Technical Specification Sirini e Dura uox dv xe Eu rR UD D 77 General SDCCHICATONS PETIT T TR 77 FP CHOMNANCE SDCCITICANONS C A A M 78 SACL SOCCIN CATIONS snes ues eu esac cy coc ca e
3. Pollution degree 2 see Safety specifications Method of disposal Electronic waste Contaminated waste Infectious waste Do not treat electrical and electronic equipments as unsorted waste Collect waste from electrical and electronic equipment separately Regarding the original packaging and packing materials use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative 76 Chapter 14 Technical Specifications General specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program Table 15 General specifications General specifications Overall dimensions ca 290 mm W x 400 mm D x 220 mm H 11 4 W x 15 7 D x 8 7 H Operating conditions 10 C to 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C non condensing Indoor use only Transportation conditions 40 C to 70 C packed in transport packaging Display High contrast color display with 480 x 272 dots Keypad Four arrow keys OK button three function keys F1 F3 FILE and HELP keys 0 9 number keys a z letters with the number keys C key START STOP and PLATE in out buttons Internal software or PC control with Skanlt Software Computer interface USB 1 1 2 0 compatible Plate types 96 and 384 well plates Maximum
4. e Ignore from end Ignores a defined number of points readings counted from the last reading e Window The number of consecutive readings to use for evaluation Time to change The time to change is used for calculating the time required to reach a defined change in the signal in each well The time is given in seconds or minutes from the first reading 32 First define the base value by entering the number of readings from the start in baseline The base value is the average of results of the given baseline count from the beginning or end of results Then select whether the change from the base value is evaluated as a relative or an absolute value and define that percentage or value This change is then added to the base value to create a required change value The average of the sliding window is compared to the limit The result is the exact interpolated time where the given change occurs You can define the following settings Processing Preprocessing kinetic param Baseline readings 100 Ignore from beginning 0 Readings from Beginning Ignore from end 0 Change 0 00 Window 2 Change type Absolute Reaction Undefined 4h Move OK Edit e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading e Window The number of consecutive readings to use for evaluation e R
5. Maximum of well The maximum of well is used to search for the maximum measurement value in each well 33 You can define the following settings Preprocessing kinetic param Maximum of we Ignore from beginning Ignore from end Move OK Edit e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading Time to maximum The time to maximum is used to calculate the time elapsed before the maximum measurement signal in each well is reached You can define the following settings eee Ignore from beginning Ignore from end Move OK Edit e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading Calculation parameters You can define the calculation parameters in the Calculation window The plate layout parameters have to be set prior to measurement The parameters cannot be changed after measurement The available calculation types are curve fits and factor You can select different calibrator curve fit types for quantitative calculation or a factor You can also use a stored curve for quantitative calculation Processing Calculation parameters No Linear regression Four parameter logistic Cubic spline Point to
6. related by the equation 35 y a bx where y denotes the expected value The minimum number of calibrators for this fit type is two Extrapolation is only available for the linear regression fit type The extrapolation result is merely indicative and needs to be confirmed Results using extrapolated data are marked with in list format In matrix format the extrapolated results are not marked Four parameter logistic The four parameter logistic method uses the fit model below b ysb 1 xc In the equation above y is the signal x the concentration a the response at high asymptote b the response at low asymptote c the 1 concentration at the curve s inflection point i e where the curvature changes from concave upwards to downwards or vice versa and d the slope factor at f c The minimum number of calibrators for this fit type is five The Concentration logarithmic transformation is not available for this fit type Extrapolation is not available for the four parameter logistic fit type Cubic spline This method is a smoothed point to point method where the adjacent calibration points are connected together using cubic polynomials see the equation below and optimizing the connecting points as smoothly as possible to avoid sharp angles The results are calculated by first searching for the correct interval and then using a bisectional method to find the answer from the corresponding equation The minimum number of calibr
7. the assay will start Select the Time hh mm ss item using the Down arrow key and press the OK button Select for example 5 min 00 05 00 as the incubation time by using the number keys and then press the OK button 48 Incubation parameters Temperature C I Wait for warm up Yes Temperature Time hh mm ss 00 05 00 m Time Move OK Edit Accept Cancel 6 Press the F1 key to accept the incubation parameters Programming a plate layout You can start filling the plate layout on any of the wells of the plate You start the fill procedure from the well marked with a blue square You can start filling a series of samples by pressing the OK button in the highlighted well You can edit and delete a single well with the Edit function The plate layout parameters have to be set prior to measurement The parameters cannot be changed after measurement In this example e ablank sample e Calibrator 1 Conc 1 e Calibrator 2 Conc 2 e Calibrator 3 Conc 3 e acontrol sample and e 91 unknown samples are programmed To create the plate layout of this example protocol 1 Select the Plate layout row in the Processing menu using the Right ok arrow key Then press the OK button to open the Layout window ff y Processing Well A1 Name EMPT Y OO OOCOQOQO0Q0 OOCGGOOOOOOOCOCO sCOCOOOOOOOQOOCOCO OOOOOOOOO0O000 2 4 Move OK Add Accept Cancel Edit 2 Press the OK
8. Celsius C Processing menu You can specify the calculation parameters in the Processing menu These parameters supplement the parameters programmed in the Main menu The Processing menu contains the following parameters e Plate layout with well information e Preprocessing for example kinetic calculations e Calculation for example curve fit e Interpretation for example limit calculation e Quality control rules for quality control 25 fi Processing Results Settings Plate layout Preprocessing Calculation Interpretation Quality control 4 4 Info text bar See the info text bar for required actions Plate layout parameters You can set the plate map of the protocol in the Plate layout window The calculations of the results are made based on the plate layout The plate layout parameters have to be set prior to measurement The parameters cannot be changed after measurement Processing Well A1 Name EMPTY 24 Move OK Add Accept Cancel Edit L Blank L Calibrator L Control L Unknown L Empt Po Fil direction L Right pL Replicate direction _ L Right po Number of L1 t Replicates Oo S Ld Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol You can start filling the plate layout on any of the wells of the plate You start the fill procedure from the well marked with a
9. E I T TETT 38 Interpretation and limits cccccccccccsecceeccceeccucccueccucccucceueceueceueceueceueceusceueseussueseesseeeseeeseeeseessaeees 38 Qualitv control Daramelels sssini a a a e c Sl Ven ane 39 RESUS MEN Ucero aaa M R 40 Raw dalda a NT MC 40 ICH ANC eo SON Si IEEE T ET ETT eee eee eh ae henna ethane REIR 41 CaliDFato A eI MET E PEE 41 Quality COMO QC mr TE 42 FASS UES VON WISE POL IINE ETT eee Gn cece 42 SOLUS THOU ete eee MeN eee gE SNE o M bU DU DU eR Ru on 43 Chapter 6 Starting Ready made Protocols 1 eee eeeee Lees eese eene nennen nnn nnns 44 Starting a ready made protocol with the quick keys F1 F3 esses 44 Starting a ready made protocol from the list csse eene nennen nnn nnns 45 Chapter 7 Protocol Creation Examples ire corsa vescedecus u spar Oca eoe Sese or Eo eu exco Ende EB ee vv nnmnnn mnnn 47 Opening a Tew DOl OCON Rc CP 47 Progamming a wavelength eet 47 Programming shaking parameters ccccccceccceecceecceeeceeceeecueseeeceeesaeeseeeseeeseeeteeessesseesseeeseeeseeeseees 47 Programming INGUDAUON Y 48 Progamming a plate JJaVOLL asivisetieictumebeaeeu iu Prec me Dros ratu Pere a Deere oc du Dacre ad D 49 Programming calculations and IT TerDEelatiOris suse is us eas Reis us ne Naso s tus nU RS Cu No dw etd NRURS DM
10. Wait for warm up Time hh mm ss 00 00 00 Move OK Edit Accept Cancel The Incubation icon and row is visible in the Main menu of the instruments with an incubator indicating the present temperature The temperature range is from 25 to 50 C in increments of 1 C Refer to Table 17 When the protocol temperature is set it is used as the incubation temperature during measurement After measurement the background temperature is taken into use again If Wait for warm up is set to Yes the assay incubation is not started until the temperature is reached During measurement the background temperature is used if the protocol incubation temperature Main gt Incubation is not in use No Background incubation is also possible during kinetic measurements When the background incubation Settings Startup Temperature temperature is set the incubation temperature is activated once the instrument is switched on Note that the cooling of the incubation chamber takes time If you get the warning of Temperature is not changing it means that the incubator chamber is cooling slowly It is possible to start the run despite of the warning message in case the incubation temperature is not critical for the application The limits for the incubation time are 00 00 00 99 59 59 The temperature in Kelvin is calculated according to the following equation K t 273 16 where K denotes Kelvin and tthe temperature in degrees
11. You can change the date in the System settings The Left and Right arrow keys are used to move from one position to 9 another in the Date setting Enter the values with the number keys o M Changing the language You can set the internal software language by moving to Language and selecting the preferred language from the drop down list 1 In the Main menu select the Settings menu using the Left arrow key 2 Select the System row and press the OK button 62 3 Select the Language item using the Down arrow key and press the OK button i Settings 1 System parameters Date 08 07 2008 10 57 38 4 MultiskanFC Date format 20 Version 1 00 49 PZ Chinese SN Empty Time format Francais French 2 Deutsch German Language EJ2K88 Japanese Portugu s Portuguese PycckWh Russian Espa ol Spanish Move OK Select Cancel Select the internal software language for example Francais using the Down arrow key and then press the OK button to accept the selection Press the F2 key to confirm the selection and to close the system parameters Return to the Main menu using the Right arrow key Filters Multiskan FC comes with installed filters of 405 nm 450 nm and 620 nm You can introduce a filter to the internal software by moving to the Filters settings The filter wavelength must be between 340 and 850 nm The Filters settings window shows the current filters of the filter wheel I
12. blue square You can start filling a series of samples by pressing the OK button in the highlighted well You can edit and delete a single well with the Edit function 26 Well type Fill direction Number of S Move OK Select Cancel Note Blank subtraction is made automatically if the plate layout includes blanks The blank 1 used in the calculations is always an average of the blanks included in the layout You can determine the sample type in Well type The sample types are Blank Calibrator Control Unknown or Empty Note The results will not be calculated for the Empty sample type Note The calibrator concentrations are programmed in the Plate layout window You can 1 define calibrator concentrations when you create a calibrator series or activate a single calibrator A calibration concentration of 0 is not accepted Fill direction can be either to the right or downwards from the active well in the Plate layout window Fill replicate direction can be either the same or different from the fill samples direction in the Plate layout window You can define the number of samples belonging to the group for example the number of different calibrators or the number of different unknown samples in Number of You define the number of replicates for each sample type in Replicate using Add series starting from the well programming Use the F3 Edit key to program each Note The Replicate number is the same for samples
13. button to start filling the plate from well A1 49 Select the Well type item in the Add series starting from well A1 window and press the OK button Select for example a Blank sample using the Up arrow key and then press the OK button Well type Fill direction Number of Move OK Select Cancel Press the F1 key to accept the selection and return to the Layout window well A2 Select well B1 using the Down and Left arrow keys and press the OK button to start filling the plate from well B1 Select the Well type item in the Add series starting from well B1 window and press the OK button Select for example a Calibrator sample using the Up arrow key and then press the OK button Select the Number of item using the Down arrow key and press the OK button Select for example 3 by using the number keys and then press the OK button d Processing V Add series starting from well B1 Well type Calibrator Fill direction Down Replicate direction Down Number of EI Replicates 1 Move Ok Select Cancel wx 11 Press the F3 Conc key to program the concentrations for the calibrators 12 Select the Cal 1 item using the Down arrow key and press the OK wv ok button 50 Enter for example 7 1 00 as the Cal 1 concentration using the number keys and then press the OK button Select the Cal 2 item using the Down arrow key and press the OK button
14. for the sample result in the category of higher than Limit 2 for example High pos The interpretation limits can be entered with an editor The editor contains the following items e Sample You can select a sample to be used for a limit calculation The Sample types can be blank BLA Calibrator CAL1 CAL2 or Control CTRL1 CTRL5 If the sample has replicates the average value of these is automatically selected Both raw and calculated data of a sample can be used as criteria e Operator One of the following can be chosen to be used in the formula or e Constant is the numeric value used for the calculation A constant can be entered with the number keys on the keypad e Function can be a standard deviation SD or a coefficient variance percentage CV SD calculates the standard deviation of the sample CV calculates the coefficient of variation for the sample e Delete deletes the created formula digit by digit 38 Interpretation 3 e g high positive Limit 2 Interpretation 2 e g positive Limit 1 Interpretation 1 e g negative Figure 17 Interpretation and limits For instructions on how to program an interpretation to the protocol refer to Programming an interpretation Quality control parameters Quality control QC is used to program rules for quality control and thereby ensure that the assay works as expected You can set a maximum of four QC rules per protocol Processing R
15. hea heal hea MMeal Nea Meal Meal Meal Meal 29 Kinetic calculations Kinetic calculations can be used when the number of readings is more than 1 in the measurement parameters see Measurement parameters Also the results of a kinetic calculation can be used as source data for further calculations The available kinetic calculation types are e Average rate e Maximum rate e Time to maximum rate e Time to change e Maximum of well e Time to maximum Note If the message Kinetic processing failed appears check the calculation parameters first Average rate Average rate is also known as normal rate The average kinetic rate slope of the absorbance Abs vs time curve is calculated by linear regression linear least squares method or LLS using all the measurement readings within the selected raw data and time range You can define the following settings f Processing Kinetic rate Ignore from beginning Ignore from end Move OK Edit e Kinetic rate Select to view the results in either seconds s or minutes min The Kinetic rate is always calculated per second but if you select per minute the time is automatically converted to minutes after the calculations e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading Maximum rate If the maxim
16. instructions e Use the original packaging for shipping e Enclose a dated and signed Certificate of Decontamination see Appendix B both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your local Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Disposal of the instrument If the Multiskan FC is exposed to potentially infectious chemical samples toxic or corrosive chemicals or radioactive chemicals waste management of the complete instrument must be carried out to ensure that there is no risk of contamination Warning Decontaminate the instrument before disposal Refer to Decontamination procedure Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Warning The used lithium Li battery is regulated waste and must be disposed of according to local regulations The Li battery has to be changed by an authorized service technician only Instructions for changing the Li battery are described in the service manual Warning Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country
17. parameters Enable quality control Raw BLA D 100 24 gt Move OK Edit Accept Cancel 35 1 Select the Quality Control row in the Processing menu using the Down wv ok arrow key and press the OK button 2 Press the OK button on the Enable quality control and select Yes using the Up arrow key and then press the OK button 3 Select the Rule 1 row using the Down arrow key and press the OK wv ok button m 4 Select Sample and press the OK button E vE mE ul wxyz 5 Select BLA blank and press the OK button 6 Select Raw and press the OK button 7 Select Operator using the Right arrow key and press the OK button 8 Select using the Down arrow key and press the OK button 9 Select Constant using the Right arrow key and press the OK button 10 Enter the constant for example 0 700 using the number keys and press the OK button 11 Press the F1 key to accept the Rule 1 formula Note The maximum number of QC rules is four If additional QC rules are needed the assay 1 performance has to be checked separately wwe 12 Press the F1 key to accept the Quality control parameters and to return prt to the Processing menu Saving a new active protocol 1 Press the FILE key in the Main or Processing menu 2 Select Save As using the Down arrow key and press the OK button The wv ok Save Protocol As dialog opens 56 3 Enter the protocol name for exampl
18. point Factor Move OK Select Cancel 34 Note Blank subtraction is made automatically if the plate layout includes blanks The blank used in the calculations is always an average of the blanks included in the layout Note The plate layout must include calibrators and corresponding calibrator parameters such as concentrations to enable them for further calculations for example curve fits Refer to Curve fits and Programming a plate layout Den Table 9 Processing parameters Calculation Processing Calculation Calculation L Type ee ee L Linear regression T conc Transfor NE L Logarithmic meas masor 00 NEN EN L Logarithmic ewon 00 L Yes L No L Cubic spline L Conc Transfor L Four parameter logistic EE WEE Lo m L Logarithmic b Meas Transfor L Logarithmic Ls NENNEN T conc transfor SSS O MEN GEM L Logarithmic LMeas Transfor L Logarithmic ee ee LVaue y O oo E100 000 0 Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol L Point to point L Factor Curve fits The curve fit types of the calibrators are e Linear regression e Four parameter logistic e Cubic spline e Point to point Linear regression Linear regression is a term usually reserved for the simple linear model involving a response y that is a continuous variable and a single explanatory variable x
19. protocol exists Chapter 7 Protocol Creation Examples This section provides examples on how to open a new protocol how to program main parameters wavelength shaking and incubation the plate layout and precalculation calculation and interpretation parameters and how to save the protocol Note The Incubation row is only active with the Multiskan FC with incubator model Processing Results Settings Protocol Untitled C3 Plate format 96 Wells 4 Measurement 405nm 1 reading E Shaking No 370 C Incubation No Move OK Edit Demotl Demo2 Demo3 Note Ensure that you save the changes you make in each step Refer to Saving a new 1 active protocol Opening a new protocol Fite 1 Press the FILE key in the Main menu ok 2 Select New and press the OK button Programming a wavelength Select the Measurement row in the Main menu using the Down arrow key 1 wv ok and press the OK button Press the OK button at the Filter 1 nm item and select for example 450 2 ok wv ok as the filter 1 value using the Down arrow key and then press the OK button E E Press the F1 key to accept the selection and return to the Main menu Programming shaking parameters m 1 Select the Shaking row in the Main menu using the Down arrow key and press the OK button Press the OK button at the Mode item and select for example Before measurement as the shaking mode using the Down arrow key
20. stable due to autoblanking procedure Table 17 Incubator Performance specifications Incubator Temperature range Ambient 4 C to 50 C in increments of 1 C Liquid warm up time Liquid warm up time lt 40 min from 25 C to 37 C 96 well plate 200 ul water well Uniformity 1 C across the plate at 37 C 78 Safety specifications This section describes the safety specifications for the Multiskan FC instrument In conformity with the requirements Multiskan FC bears the following markings Type 357 100 240 Vac 50 60 Hz 100 VA max CE marking CSA monogram or similar The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Up to 2000 m Temperature 5 C to 40 C Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains supply fluctuations 10 from nominal Installation category Il according to IEC 60664 1 see Note 1 Pollution degree 2 according to IEC 60664 1 see Note 2 Humidity Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable t
21. the number keys and then press the OK button Press the F1 key to accept the selection and return to the Layout window The filled wells will show the following colors for blank wells green for calibrators bright green for controls and blue for unknowns Processing Al ce Well At Name BLA Replicate 1 1 QOOOOOQOO QOOOQOOCOO QOOCOQOOOCOQQO OOQOQ QO O00 OK Add Accept Cancel Edit Delete vwm 0 Press the F1 key again to accept the plate layout and to return to the Processing menu Programming calculations and interpretations Programming precalculations Preprocessing carries out calculations that are normally made as a first step in result handling that is preprocessing and kinetic calculations Precalculation is used with dual two wavelength measurements for example subtracting the reference wavelength from the assay measurement wavelength Processing Results Settings Plate layout B Preprocessing 3 ae Calculation 1 Select the Preprocessing row in the Processing menu using the Down arrow key and press the OK button 2 Select the Precalculation type and press the OK button Select for example Mea1 Mea 2 using the Down arrow key and press the OK button 52 Processing Preprocessing parameters Precalculation Move OK Select 4 Press the Left arrow key to return to the Main menu Programming calculations 1 Select the Calculation row in the Pro
22. 05nm 1 reading shaking 37 0 C Incubation Move OK Edit Demo1 Demo2 Demo3 Note Use only plates manufactured according to ANSI SBS standard dimensions The dimensions of the available plates are given in Table 5 and Table 6 Table 5 96 well plate dimensions 96 well plate Plate height Plate length Plate width First well position X First well location Y Corner distance X 199 Table 6 384 well plate dimensions 23 Measurement parameters You can define the measurement filter s number of readings and measurement mode in the Measurement window Filters Filter 1 defines the first main filter wavelength Filter 2 defines the second reference filter wavelength Measurement parameters Filter 1 nm 05 No of readings 1 100 Filter 2 nm No filter Mode Fast 4 Move OK Edit Accept Cancel You can select the suitable filter from the list of available filters You must physically install the filter into the filter wheel as well as introduce the additional filter to the internal software of the instrument in the Settings window Refer to Introducing a filter to the internal software Measurement modes There are two measurement modes Normal and Fast The measurement head stops on each well in the Normal measurement mode The Normal mode produces the most accurate results with measurement times that are fast enough for most purposes The head moves continuously in the Fast
23. 4 gt Move OK Edit Close The order of the data views results to be shown is according to the following list depending on which calculations were used in the protocol e Assay quality control if failed The results of a failed run are flagged e Interpretation e Quantitative results e Precalculated results e Raw data mwewm l Press the F3 Menu key to enable menus of different data views in order to view them 57 Select another data view using the Up and Down arrow keys and press 2 a wv ok the OK button Press the FILE key in the data view to print or export a data view Select 3 wv ok Export As Text or Print using the Down arrow key if necessary and then File press the OK button mwwm 4 Press the F2 key to close the data view F2 Processing f Results 1 Settings Raw data f Abs Calculated results Calibration curve Quality control Results in list format Demo2 Demo3 Note It is possible to select which information to be exported or printed by pressing the FILE 1 key Refer to Chapter 9 Exporting and Importing To view another row from the Results menu using the Up and Down arrow keys and press the OK button To view different data views or to print refer to Steps 2 4 Press the F2 key to close the data view Press the Left arrow key to return to the Main menu Chapter 9 Printing Exporting or Importing It is possible to export and import the stored protocols
24. 52 Programming precalculations cccccccecccscccscccsecceeccscccseccseccauecueccaeecauecaeecseecaeeeueecueseueseeeseeesnass 52 Programming calculation Sessea a a Era rotten 53 Programming an interpretation ccccccecceccseceseeeneeeseeeneeeneeeneeeneeesueeseeeneeeneeeseeeneeeseeeseeeneeeneeenaes o3 Adding d QC rule o INC DIOIOCOL a oie rater uvas rte autero abe cepas usta tod Dite tui 55 Saving a NeW active ProlOCO iseis ead see 56 Chapter 8 Viewing AESUINS we tsscivwsestetsecuacsaxwciccusneisiedonwadeasenwusnedenvuducasnwusiedatvndedasnueseadseeudecemnwesdidenuuss 57 Chapter 9 Printing Exporting or Importing ccccesseeceeseeeeseeeeeseseeseecensesenneseensesoaneseeneesonees 58 Printing Or CXOOMING oz METRE TET 59 EXPOING aA PrO OCON re n E 60 IMPpPOnmng a proloco nere 61 Chapter 10 SUG OW IN eraen E ceca Ene Ve neas cu Sce D E 62 Chapter 11 Modifying Settings ooo biooe tea Eubo sus an eua ar ho nne lun suia rb oou ae lan prsrAVvb o ceac bun nnmnnn nnmnnn sua Sn 62 oSVStenm SCTIAOS sexes Sees oa OA Sites hk dile Gants Saar IM RE ILU dea eee S LI i eie 62 RS UO Oc TTE 62 Ghianglhg the languages inna paste t d a Te ptus ue qiue ua tontesbancdshouetegauehsuedshountegteahsaedsteunteceanbenauchete 62 miM E 63 Introducing a filter to the internal software sees nnn nnne nnns 63 Removing a MLS m re ree 64 utc T TP
25. 80 2 062 2 274 2 309 H 2 132 2 239 2 439 2 275 Move OK Edit 40 You can disable enable samples and sample replicates from the raw data by pressing the OK button Select Disable and press the OK button If the user enables automatically disabled data the user is responsible for data quality You can print or export the selected data For further information refer to Chapter 8 Viewing Results Calculated results The calculated results are shown in the Interpretations window Processing Results Settings Interpretations Protocol De10 10 09 2008 13 24 00 AT CTRL1 Filter 1 405nm 1 2 9 10 11 12 AE TTE Nes Nes Nes Nes Nes Nes Nes B Nes C Nes D Nes E Nes F Nes G Nes H Nes In situations where the result cannot be calculated from the available data the result is expressed as NaN quantitative or Undefined qualitative Examples of such situations are e The divisor is zero 0 e The kinetic calculation cannot be processed e Concentrations for extrapolated data cannot be calculated when extrapolation is disabled e The qualitative range is undefined e f previous calculation results are NaN Note Blank subtraction is made automatically if the plate layout includes blanks The blank used in the calculations is always an average of the blanks included in the layout Note The results of precalculations are not available in the data view They are only available in exported or printed re
26. D OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The seller is not liable for any loss or damage arising out of or in connection with the use of the product or other indirect damages Cat No NO7710 January 2015 About This User Manual Intended users The Thermo Scientific Multiskan FC as standalone or with Thermo Scientific Skanlt Software can be used in research and routine test laboratories by professional personnel How to use this user manual This user manual is for the following instruments Multiskan FC Multiskan FC with incubator Cat No 51119000 51119050 51119100 and 51119150 It has been designed to give you the information you need to e Review safety precautions e Install the Multiskan FC e Navigate and edit in the Multiskan FC user interface e Make a protocol and measure e Perform basic cleaning and maintenance procedures e Troubleshoot the instrument performance This user manual also describes all the features and specifications of the Multiskan FC instrument as well as ordering information Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available during use of the instrument Keep the user manual together with the instrument in case you distribute it onwards For more informat
27. Enter for example 2 2 00 as the Cal 2 concentration using the number keys and then press the OK button Select the Cal 3 item using the Down arrow key and press the OK button Enter for example 3 3 00 as the Cal 3 concentration using the number keys and then press the OK button Concentrations Unit Cal 1 1000 Cal 5 0 000 Calg Cal 2 2 000 Cal B 0 000 Cal 10 0 000 Cal 11 0 000 Cal 8 0 000 Cal 12 Move OK Edit Accept Cancel 18 Press the F1 key to accept the concentrations and return to the previous view i e the Add series starting from well B1 window Ti BEN 19 Press the F1 key to accept the concentrations and to return to the Layout window 20 Press the OK button to start filling the plate from well E1 21 Select the Well type item in the Add series starting from well E1 window and press the OK button 22 Select for example a Control sample using the Up arrow key and then press the OK button 23 Press the F1 key to accept the selection and return to the Layout window 24 Press the OK button to start filling the plate from well F1 25 Select the Well type item in the Add series starting from well F1 window and press the OK button 51 Select for example an Unknown sample using the Up arrow key and then press the OK button Select the Number of item using the Down arrow key and press the OK button Select for example 97 using
28. FAT32 4 fthe item is a virtual CD drive then the type will be CD Drive This may cause the USB memory device to be incompatible with the Multiskan FC 81 Warnings and cautions This instrument is designed to provide full user protection When correctly installed operated and maintained it will present no hazard to the user The following recommendations are given for added user safety Electrical Ensure that the power supply cable supplied with the unit is always used If a correct type of mains cable is not provided use only cables certified by the local authorities The power plug should only be inserted into a socket outlet provided with a protective ground contact Never use an extension cable without a protective ground wire Warning Only authorized technical service personnel are allowed to open the instrument Disconnect the instrument from all voltage sources by disconnecting the power supply cable before opening it The same precautions applicable when using any electrical equipment should naturally be observed with this instrument Warning Do not touch switches or electrical outlets with wet hands Switch the instrument off before disconnecting it from the mains supply Defects and abnormal stresses perturbation which could impair the operation of other devices or equipment in the usual Warning If the instrument is not functioning properly it may create electromagnetic laboratory environment Whene
29. SERVICING and AVERTISSEMENT COUPER L ALIMENTATION AVANT L ENTRETIEN ET LE DEPANNAGE Warning and other markings used in the documentation The following symbols and markings appear in this user manual Warning Risk of electric shock A AN A Warning Biohazard risk Warning Hot plate risk of burns Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest Table of Contents Chapter 1 Introduction to the Multiskan FC ccccsssessseecsseeeeseeceseesensesceneeeensesenseeeeneenonnenes 9 OO OU E use un Man ER MAMMA AMA AMAA AA CM E 9 PHINC OT ODE aO C 9 Chapter 2 IfistallallOH si a a a a E 10 Blolsiziei igo aenn EE 10 mie TOMO SERE PUT ER MEET NEU 10 Checking delivery for completeness or damage sessessssesssesesneeeen nennen nennen 11 Environmental reQuireMenttS cccccccccscccecccscccecccscccecccscccaccceeecseecaeecaeecaeecaeecaeecaeecueeeaeseaessaeseass 11 SOLUS mm PP edd 11 Heleasingsthie transport NOCK sc tisse ated gabe iudei es uet deubi i bres qaedi lesu Bui 11 Installing the filter wheel osse tots aed reck ek t ux tu rec Seu k Eua Du gzeck x euss vb aa aug zwck qeu e
30. Switch the power off 4 Open the lamp and filter wheel chamber cover 5 Push the transport lock bar through the hole in Figure 27 into the hole in the measurement head Figure 27 Refitting the transport lock bar into the measurement head 6 Push the transport lock bar in as far as possible Figure 27 7 Fasten the transport lock bar and tag clockwise using the Allen key supplied Figure 28 Ensure that the transport lock bar is completely tightened 74 Figure 28 Fastening the transport lock 8 Close the lamp and filter wheel chamber cover The transport lock bar and tag are now refitted Figure 29 CAUTION Remove Transport Lock Before Use Figure 29 Transport lock bar and tag refitted Maintaining a system log A system log which includes a short summary of the use maintenance procedures error messages and other information about the use of the system is useful in properly maintaining the system Refer to Appendix A System Log 75 How to pack for service To pack the Multiskan FC for service e Inform about the use of hazardous materials e Remove any microplate before decontamination Also remove the filter wheel and put it into its transportation box Decontaminate the instrument e Refit the transportation lock Refer to Refitting the transport lock e Close the lamp and filter wheel chamber cover and the measurement chamber door e Pack the instrument according to the packing
31. Thermo Scientific Multiskan FC User Manual Rev 2 2 Cat No NO7710 Copyright 2015 Thermo Fisher Scientific Inc All rights reserved Decon is a trademark of Decon Laboratories Limited Excel and Microsoft are trademarks of Microsoft Corporation in the United States and other countries Microside SQ is a trademark of Global Biotechnologies Inc Virkon is a trademark of Antec International Ltd All other trademarks are the property of Thermo Fisher Inc and its subsidiaries Reproduction of the accompanying user documentation in whole or in part is prohibited Patents This product is protected by the following patent US 6111636A Device for Measuring Optical Density Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions Remarks on screenshots ocreenshots may be slightly different on your system depending on the firmware version No liability for consequential damages Thermo Fisher Scientific shall not be liable for any indirect or consequential damages whatsoever ar
32. ality control QC You can view the quality control data in the Quality control window If the QC rules are not fulfilled and the assay is therefore not valid the QC view is not opened until after the measurement Results Quality control Rule Result 1 Raw BLA lt 0 080 0 040 lt 0 080 PASS 2 Conc CTRL f gt 20 124 FAIL You can print or export the selected data For further information refer to Chapter 8 Viewing Results Results in list format The assay results can be viewed in list format in the Results as list window Results Results as list Interpretation Interpretation Close Next page You can print or export the selected data For further information refer to Chapter 8 Viewing Results 42 Settings menu You can set the instrument parameters in the Settings menu The values shown in the Settings menu remain in the instrument memory and are instrument specific not protocol specific You can define System Filters Printer and Startup settings For instructions on how to modify different settings refer to Chapter 11 Modifying Settings Processing Settings st 21 Sustem a Filters Printer Startup Move OK Edit Demol Demo2 ae e Info text bar a L 22 09 2008 14 19 01 L Date format L dd mm yyyy L dd mm yyyy L dd mm yyyy L yyyy mm dd L mm dd yyyy L 12 hour L 24 hour L Language L English L Russian L French L Sp
33. alysis interpretation the results are divided into separate categories The data is categorized using cutoff values Limit 1 and Limit 2 for up to three different categories Figure 17 The limits cutoff values can be set as a number a formula a specified sample or a formula containing both samples and numbers 37 The absorbances or concentrations of the samples are compared to the limits and defined for example as negative or positive Source data You must define the Source data for the interpretation calculation The source data used for interpretation can include the following data types e Raw absorbances Raw e Blank subtracted absorbances Blank e Precalculated absorbances Dual e Preprocessed kinetic values Kinetic e Calculated concentrations For further information refer to Preprocessing parameters Interpretation and limits Interpretation parameters Limit 2 Interpretation 2 Pos Limit 1 Raw CTRL1 0 050 Interpretation 1 Neg Source data Move OK Edit Accept Cancel An interpretation calculation always contains at least one limit and two categories A limit can be a value or a formula Refer to Figure 17 Interpretation 1 is a typed name for the sample result in the category less than Limit 1 for example Neg Interpretation 2 is a typed name for the sample result in the category of higher than Limit 1 but less than Limit 2 for example Pos Interpretation 3 is a typed name
34. and then press the OK button 47 vE vE vo H BE Select the Speed item using the Down arrow key and press the OK button Select for example Fast as the shaking speed using the Down arrow key and then press the OK button Select the Time hh mm ss item using the Down arrow key and press the OK button Select for example 20 seconds 00 00 20 as the shaking time by using the number keys and then press the OK button Press the F1 key to accept the selection and return to the Main menu Programming incubation You can set the incubation time and temperature in the Incubation window 1 lil Note The Incubation row is only active with the Multiskan FC with incubator model Note The temperature set in the protocol overrules the startup temperature If the protocol 1 does not include incubation the startup temperature remains Processing Protocol Plate format Measurement shaking Incubation Results Settings Untitled 96 Wells 405nm 1 reading No No Select the Incubation row in the Main menu using the Down arrow key and press the OK button Press the OK button on the Temperature row and use the Down arrow key to select the preferred temperature from the list The temperature range is from 25 to 50 C in increments of 1 C Then press the OK button If Wait for warm up is set to Yes the assay will not start until the temperature is reached If Wait for warm up is set to No
35. anish L Chinese L Japanese L Portuguese b Wavelength Filters L Wavelength L 405 1 L 450 2 Lh ot L Comment a ee ooo SS pe Printerparameters ef t Enable header 43 L For example Laboratory 123 i SE S L Plate position a e L Out e Temperatureec os L No L 25 50 Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol Chapter 6 Starting Ready made Protocols Note Make sure the lamp and filter wheel chamber cover is closed before starting a 1 measurement Starting a ready made protocol with the quick keys F1 F3 Use the F1 F3 function keys reserved for ready made demo or favorite protocols to measure routine assays quickly Three default protocols can be connected to the F1 F3 keys You can change these protocols from Protocol OK FILE Quick Key Set Clear F1 F3 SEMEL Processing Results Settings Protocol Untitled Plate format 96 Wells Measurement 405nm 1 reading shaking No Incubation No 1 Press for example the F1 key Demo7 in the Main menu 2 If the plate carrier is inside the instrument press the PLATE in out button Insert the assay microplate so that the A1 corner is positioned in the top left corner of the plate carrier Figure 18 Figure 18 Multiskan FC plate carrier Warning The plate carrier opens automatically Make sure there is enough clearance
36. asurements 31 1 to 3 the second rate using the measurements 2 to 4 and so on up to measurements 8 to 10 The maximum rate will be the maximum value among these calculated rates Time to maximum rate The time to maximum rate is calculated similarly to the maximum rate but the result is reported as the time in seconds from the first reading to the point on the line where the maximum rate occurs instead of the rate itself Refer to Figure 16 1 4 1 U B 0 6 0 4 0 2 D Abs Time s Time to Max Rate Figure 16 Determining the time to maximum rate You can define the following settings A Processing Preprocessing kinetic param Ignore from beginning 0 Ignore from end 0 Window 2 Reaction Undefined 4 gt Move OK Edit Accept Cancel e Reaction Define whether the reaction is supposed to produce increasing or decreasing signal levels There are three options for this setting e Undefined Searches for the absolute maximum rate and returns the corresponding time e Increasing Searches for the maximum increasing rate and returns the corresponding time If no increasing rate is found the result is NaN not a number e Decreasing Searches for the maximum decreasing rate and returns the corresponding time If no decreasing rate is found the result is NaN not a number e Ignore from beginning Ignores a defined number of points readings counted from the first reading
37. ators for this fit type is five yi 8 ji T bx CX dx Only the means of the measured calibrator replicates can be used not the individual replicate values Extrapolation is not available for the cubic spline fit type Point to point The point to point method connects the adjacent response concentrations coordinates that is the calibration points together using a straight line see the equation below which is different for each of the intervals The results are calculated by first searching for the correct interval and then using the corresponding equation The minimum number of calibrators for this fit type is two yi aj DX Only the means of the measured calibrator replicates are used in the calculations The replicates are visible in the graph but they are not used in calculations Note The method always returns the first value found for a sample Therefore ensure that the pE calibration points behave monotonously Extrapolation is not available for the point to point fit type Factor Measured absorbances are multiplied with a factor given by the user to obtain the concentrations The concentration is calculated according to the following equation Concentration Factor x absorbance Stored curve You can use a stored curve for an assay a run You can only use a stored curve with the protocol it was created with After the curve has been stored the plate layout may be modified to contain for example only unkno
38. atrix Results as list Move OK Select Accept Cancel wm 6 Press the F1 key to accept the desired data xn Data is exported to the USB memory device or printed to the external printer depending on the selection Run information includes all protocol and processing parameters The naming of an export file is automatic with a running number that is the instrument searches for the first number available Excel Note The internal software uses 32 bit floating point numbers in processing lil Note It is recommended to format the USB memory device if export fails For more information lil Note The data format should be UTF 8 when the txt file is opened in for example Microsoft refer to Chapter 15 Troubleshooting Guide Exporting a protocol The calculated results or stored protocols can be exported to the USB memory device via the USB port Figure 9 by using the FILE key Runs including protocol information and data can be exported Protocols can be exported and imported to another Multiskan FC instrument with the same configuration Import of runs is only possible to the same instrument as backup In the main level of Results the results or protocols to be exported can be defined by FILE Export A protocol can be transferred from an instrument and imported to another instrument 1 Insert the USB memory device into the USB memory device port of the instrument 2 Press the OK button on the P
39. background temperature is taken into use again Background incubation is also possible during kinetic measurements AN Warning The incubator thermal plates inside the instrument may be hot Shaker The linear shaker operates at three different speeds Table 1 Table 1 Shaking speeds Speed name Slow 5 Hz amplitude 15 mm 11 Hz amplitude 3 mm 20 Hz amplitude 1 mm Background shaking is also possible during kinetic measurements Internal memory There is memory for 99 assay protocols and at least assay results for 100 microplates Chapter 4 Operating the Internal Software Display and keys for navigating and editing The keypad and display are shown in Figure 13 18 SCIENTIFIC MULTISKAN FC Menu Active row blue Untitled Plate format 96 Wells Display l Measurement 405nm 1 reading Shaking Incubation Info text bar Function keys abc in out 5 m jkl Number keys with letters 2 Arrow keys and OK key START STOP and PLATE in out keys Figure 13 Keypad and display of the Multiskan FC Keys Action The relevant keys for navigating and editing are described in detail below The keys also have other functions depending on the level in the internal software The active row is colored blue Use the Left Right Up and Down arrow keys to navigate You can i v speed up the selection by holding down the arrow key continuously ok Press the OK button to select and edit t
40. c nae i na Eu E 79 In conformity with the requirements ccccccecccscccecccscccecccececsceceeecseecaeeceeeceeecseeceeeseeenseeneeeneeenes 79 Chapter 15 Troubleshooting GUIOG uestes cente cm ie inno Dus te cus iue ee vau Uo Sia Casin acea vUe aia dave E cera ons Ex cea cene 79 Eror and warming CodeS TETTE T T 79 JSB MEMON OVCE ea TOT EOD E EEO TET 81 D Os ad Calon sesan E E UE IC UI E Oe Ee en 82 mis ej ewe ee T SC 82 Defects and abnormal SIress65 uoo roS eboney iat elas cuin s putsis eai esee uoce oui doit 82 Chapter 16 Ordering Inforrmiatlorn iiec ooi eser eene eot eue paene eos e exeo PCR nex e Unas coe o pee nere oaS RE oa CERE EE 83 BTS OP IOS ice acdsee acetates cascada acetate tud usb stel eid auta etme 83 EISt Of Spare Darts ANG ACCESSONES a wc ec OE cetera Gee OR UU PEOR LR OR it OR a a3 List or Thermo Scene DIGIGS 2 oc tcu utt Ru tu acte du ieu de Ser Rl eden tas Dade teu er M tenes abl 84 VEMMCAUOM tools astuce leuita ipu ibl INIM I I IMMER I MN ERI D RM ELI ERE 84 Appendix A System Meroe nw S S S 85 Appendix B Certificate of Decontamination ccccsceeseeseeeeeeceeeeeeeseeeeneeeenseseeseeseneeseeseesonees 86 Chapter 1 Introduction to the Multiskan FC The Multiskan FC Figure 1 is a high quality filter based microplate photometer It is used to measure absorbance from appropriate 96 or 384 well plate formats in the wa
41. cessing menu using the Down arrow key wv ok and press the OK button m 2 Select Type and press the OK button 3 Select for example Linear regression as the calculation type calibrator curve fit wv ok using the Down arrow key and then press the OK button Processing Calculation parameters Four parameter logistic Cubic spline Point to point Factor Move OK Select Cancel www 4 Press the F1 key to accept the selection F1 5 Press the Left arrow key to return to the Main menu Programming an interpretation The following example shows how to add an interpretation to a protocol The interpretation limit in this example is the absorbance of the negative control 0 05 Abs The samples below the limit are categorized as negative Neg and the samples above it as positive Pos 53 1 Select the Interpretation row in the Processing menu using the Down arrow key and press the OK button 2 Select Source data and press the OK button 3 Select for example Raw using the Down arrow key and then press the OK buiton Processing Interpretation parameters Interpretation 3 Limit 2 Interpretation 2 Limit 1 Interpretation 1 Source data Move OK Select Cancel 4 Select Interpretation 1 using the Up arrow key and press the OK button 5 Enter the category name for example Neg by using the letter keys and press the OK button 6 Select Limit using the Up arro
42. cup us s eue E nnmnnn mnnm 20 MIEN ERREUR RR REP rA 20 PO LOCO Wir Hr rr ERES TNIENER 22 Plate format parameters cccccccecccscccscccecccecccecccseccseececccacecseecaeecaeecaeecseecaeecaeecseeeaeecaeseeeseaesnass 23 Measurement parameters sssini duel ext Sev sv dul ict iwa abeiro Seakan eva erudi lad 24 mc HM T HE EE E 24 MeasSUremernt THOGGS uec nective rats rae nd n ted Lat EI dues ned de rM Der Uu 24 Shaking Dat atTielel Ss cissonu estet o ohx Msn E TE tnu escent hadi aan aces ean MIB MD ED Mx E ERES E M E EM EE 24 Incubation parameters osseesssseseeeseeeeeeee nennen nnne nnne nnne nnne nnne s nsn nsu sana nsu sese sns arse is 25 FLOCESSING INCU mM 25 Plate layout parameters issiria ERR ide EM e die thas Mo eMe ooo thee oaa a oe oa V cov o UNIS US VEU UNT 26 Preprocessing Dalal IO FS PER TE T T 27 FC CAICUIAU ONS oui adusta nac c cda Inoue ud Inc M Inc DRM DM nM 29 Knel silo rci og zo DRM 30 Calculation Dalam Glens cud sende eiubdeu dettes peed etu ehoen estable etu reno dtabiedu tus rens tab dutusue etes detubushs 34 CUVE TIO MH 35 gelo NM M o 36 EOLO eU Miele UI AULEM III re E 36 Interpretation parameters sssssssesseeeeeeeeen nennen nennen nnne nnne nnne nnne snsa esa esa esas ese esas aser 37 AS10108 LE EO TEE TENTAT NA EEIE T EEE TE ETETEA IM A A
43. d L Increasing L Decreasing a a Ee o maximum rate l Kinetic rate rate L min fe ignore frombeginning Ec E tenore from end mE EE we Lem L Undefined L Increasing 28 Processing Preprocessing L Ignore from beginning SS a 9 L Time to change L L Undefined L Increasing L Decreasing oo Baseline readings J 000 o o o o LI00 pf Leading from EMEN L Beginning L End pe hange 0 o o o o 5 R00 a bhOhmgepe 0 0 L Relative 96 b Maximum of wel Ignore from beginning o 00 O meemmed EE L Time to maximum L Ignore from beginning ae LO NENNEN Lo o o qe Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol Precalculations Precalculation is used in the context of dual two wavelength measurements when Filter 1 main filter and Filter 2 reference filter are defined in the Main menu The purpose of the precalculation is to merge the data from the two measurements into one data set which can then be further processed for example using kinetic calculations or a curve fit The most common example of precalculation is the subtraction of the reference wavelength where absorbance values of Measurement 2 measured with Filter 2 is subtracted from the absorbance values of Measurement 1 measured with Filter 1 heal Mea Meal
44. d on s Note If you have a similar data view as the one below the main view of the Results menu will open by pressing the F2 Close key Processing Results Settings Protocol Untitled 26 08 2008 20 38 14 Filter 1 340nm 3 4 5 6 8 9 10 11 12 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 4 Move OK Edit Close 2 If you are in the Main menu press the Right arrow key continuously till you get to the Results menu Processing Results Settings Abs Raw data f Abs Calculated results 26 Calibration curve Quality control Results in list format Move OK Edit Demo1 Demo2 Demo3 3 Press the FILE key 4 Select Export Results As Text or Print Results and press the OK button 5 The Define export Print box is opened Select the information you want using the Down arrow key and tick the check box with the OK button 59 3 Processing i Results Settings Define export Run information Instrument status Plate layout Quality control Results as m
45. d results Calibration curve Quality control Results in list format Move OK Edit Demo1 Demo2 aes a Info text bar The assay data that is linked to the protocol consists of raw data and all the information needed for calculations programmed in the Main and Processing menus The result of the previously run assay protocol measured protocol is shown as default if exists When another protocol is selected from the Main menu the result view is cleared to avoid any conflicts Note The instrument measures the absorbance with four decimals Absorbance data is pE presented with three decimals However all measured decimals are used in further calculations Note Results over 4 Abs are calculated but they are struck through and interpretations for 1 these results are not given Raw data You can see the unprocessed data raw absorbances of the assay in the Raw data window Results Raw data Protocol Untitled 11 12 2012 09 45 58 AT UNT Filter 1 405nm 1 2 3 4 5 6 Y 8 9 10 11 12 AMPS 2 099 2 154 2 368 3 287 2 751 2 418 2 031 2 597 2 789 3 206 2 144 B 3 454 2 713 2 029 2 208 3 068 2 066 2 208 2 413 2 594 2 128 2 010 2 338 C 2132 2 183 2 063 2 545 2 221 2 336 2 257 2 478 2 764 2 160 2 022 2 868 D 2 018 2 692 2 519 2 3398 2 238 2 735 2 797 3 400 2 379 2 684 2 771 2 217 E 2 261 2 240 2 544 3 060 2 177 2 453 2 631 2 322 2 171 2 034 2 030 2 026 F 2 535 2 082 2 882 2 14 2 010 2 249 2 011 2194 2 161 G 2 943 2 098 2 155 2 9
46. diately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled water Cleaning a filter 1 Remove particles for example dust with clean pressurized air 2 f necessary clean the filters with a lintfree tissue soaked in 7096 ethanol Caution Do not use any other liquids to clean the optics Avoid any harsh treatment 3 Wipe over the filter in a straight movement from inside to out Do not use any circular movements when cleaning the filter 4 fthe filter is not clean repeat the procedure Caution Check the condition of the filter s regularly Cleaning of the instrument Clean the instrument regularly as stated below agent as recommended by the manufacturer Do not expose painted surfaces to concentrated Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning acids or alcohols for prolonged periods of time as damage may occur owitch the power off and unplug the instrument Use disposable gloves Clean the instrument outside and the plate carrier with a soft cloth dampened with water or mild detergent 4 f you have spilled infectious agents on the instrument decontaminate the instrument Refer to Decontamination procedure Caution Do not use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as this may cause permanent damage to the f
47. e Multiskan FC instrument and accessories carefully with the arrows on the transport package pointing upwards Place the instrument onto a laboratory bench Caution Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so may cause misalignment and will void the instrument warranty Retain the original packaging for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use If you relocate your instrument or ship it for service refer to How to pack for service 10 Checking delivery for completeness or damage Check the enclosed packing list against order Visually inspect the transport package the instrument and the accessories for any possible transport damage If any parts are missing or damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Caution If the instrument has been mechanically damaged ship it for service Environmental requirements When you set up your Multiskan FC avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations Make sure e the working area is flat dry clean and vibration proof and leave additiona
48. e Test7 by using the number and letter keys and then press the OK button Processing Results Settings Plate layout Quality control Note You cannot use the protocol name Untitled Note The results of an Untitled protocol are not saved unless you save the active protocol 1 including the measured run data with a new name Chapter 8 Viewing Results The results can be viewed in several formats depending on the protocol settings raw data in list and table format calculated results in list and table format and graphs calibration or kinetic curves You can disable enable samples and sample replicates from the raw data in table format If the user enables automatically disabled data the user is responsible for data quality After the run has finished the data view of a run is automatically shown see example view below Processing Results Settings Protocol Untitled 26 08 2008 20 38 14 Filter 1 340nm 3 4 9 6 r4 8 9 10 11 12 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 0 204 2
49. e protocol list Endotoxin 17 06 2008 13 03 40 17 06 2006 13 03 40 Helicobacter 17 06 2008 13 04 44 17 05 2008 13 04 44 HIY 1 2 17 06 2008 13 05 30 17 06 2008 13 05 30 MTT 17 06 2008 13 05 54 17 06 2008 13 05 54 Move OK Load 45 2 Select the ready made assay protocol you want to run from the protocol list wv ok using the Down arrow key and then press the OK button Note The protocol name selected is shown on the Protocol row in the Main menu 3 Ifthe plate carrier is inside the instrument press the PLATE in out button Insert the assay microplate so that the A1 corner is positioned in the top left cue corner of the plate carrier Warning The plate carrier opens automatically Make sure there is enough clearance in front of the instrument for the plate carrier to come out freely 4 Press the START button 9 5 Enter the unknown count with the number keys ra to 6 Press the START or OK button to accept the selection and to start the o ok EN measurement Note If you want to cancel the run press the F2 key 7 The microplate is measured and the results are automatically calculated according to the predefined protocol Note During the run you can press the STOP button to abort the run 8 Press the F2 key to close the result table and then press the Left arrow key pexy Ey twice to return to the Main menu 46 Note The main parameters are locked when a run measured data for this
50. e timer is not anymore running Your timing Contact the PC software vendor requirement is not met 102 Temperature is not changing Please note that the cooling of the incubation chamber takes time If you get the warning of Temperature is not changing it means that the incubator chamber is cooling slowly It is possible to start the run despite of the warning message in case the incubation temperature is not critical for the application USB memory device It is recommended to format the USB memory device if export or import fails It is recommended to use FAT16 or FAT32 formatted USB memory devices It is not recommended to use multiple drive USB memory devices that contain virtual CD drives The multiple drive USB memory devices normally contain one or more removable disk type memory spaces but can also contain virtual CD drive type memory spaces The Multiskan FC does not support virtual CD drive type memory spaces It is possible to check if there is a virtual CD drive present when inserting a multiple drive USB memory device into the PC USB port 1 Select My Computer and note the new drives that appear in the list when the multiple drive USB memory device is inserted Check all the appearing items by using the right click mouse menu and selecting Properties The item type Removable Disk is stated for the USB memory device in the General sheet It is compatible with the Multiskan FC if the File system is FAT16 or
51. eaction Define whether the reaction is supposed to produce increasing or decreasing signal levels There are three options for this setting e Undefined Searches for the defined change in signal regardless of the rate polarity positive or negative and returns the corresponding time e Increasing Searches for the increasing defined change in signal and returns the corresponding time If no increasing rate is found the result is NaN not a number e Decreasing Searches for the decreasing defined change in signal and returns the corresponding time If no decreasing rate is found the result is NaN not a number e Baseline readings The Baseline readings parameter is the number of initial readings calculated from the beginning first or end last of the measurement used for the baseline calculation The baseline can also be zero which means that the comparison will be made against zero In the Baseline readings the software chooses as many measurement points as exist in the baseline and calculates the mean thereof The mean equals the baseline value If ast is selected the change is searched starting from the end of the measurement e Readings from There are two options for this setting Beginning default or End e Change The change in signal is specified and compared to the baseline e Change type The relative change from the baseline in percent or the absolute change The default is Absolute
52. esults Settings Plate layout Move OK Edit L Rule 1 L Rule 2 L Rule 3 L Rule 4 Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol 39 The rules formulas can be entered with a QC rule editor The editor contains the following items e Sample Select the sample to be checked It can be any of the calibrators or controls present in the layout or the blank If the sample has replicates the average value of these is automatically selected for the QC check Both raw and calculated data of a sample can be used as criteria e Operator One of the following can be chosen to be used in the formula gt or lt e Constant A constant can be entered with the number keys on the keypad e Function SD or CV of a sample can be added SD calculates the standard deviation of the sample CV calculates the coefficient of variation of the sample e Delete deletes the created formula digit by digit For instructions on how to add a QC rule to a protocol refer to Adding a QC rule to the protocol Results menu You can view the results of a measured run assay in several formats in the Results menu depending on the protocol settings in the Processing menu raw data in list and table format calculated results in list and table format and graphs calibration and kinetic curves Processing A ILL Settings Raw data f Abs Calculate
53. ew Side view The side view of the Multiskan FC instrument is shown in Figure 12 Lamp and filter wheel chamber cover open m m Plate carrier OU Figure 12 Multiskan FC side view USB memory device port The instrument is equipped with a USB port for an external memory device Figure 11 You can transfer measurement data to the PC and assay protocols from an instrument to another with the USB memory device 17 Note It is recommended to format the USB memory device if export or import fails For more at information refer to Chapter 15 Troubleshooting Guide Incubator The optional incubator is only available with the Multiskan FC with incubator model The incubator chamber consists of thermal elements on the top and bottom of the incubator chamber The upper electrical thermal element prevents condensation on the microplate that is being incubated The incubation temperature ranges from 25 to 50 C max in increments of 1 C When the background incubation Settings gt Startup gt Temperature temperature is set the incubation temperature is activated once the instrument is switched on The temperature is then kept constant all the time During measurement the background temperature is used if the protocol incubation temperature Main gt Incubation is not in use No When the protocol temperature is set it is used as the incubation temperature during measurement After measurement the
54. f the points mentioned in the checklist below Table 13 65 Table 13 Maintenance checklist ENS LN Keep the instrument free of dust See General AB oF of oF Avoid disturbing any of the optical system components including optical covers See General Y Clean and check the condition of the filters and the filter wheel position slot Wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces immediately to prevent damage and wipe with deionized distilled water See Immediate If any surfaces have been contaminated with biohazardous material disinfect with a mild sterilizing solution See General Clean the case of the instrument periodically See Cleaning of the instrument Clean the plate carrier when necessary See Cleaning of the instrument Ensure proper shutdown See Chapter 10 Shutdown Decontaminate the instrument when relocating the instrument or sending it for service See Decontamination procedure Perform verification with the Multiskan Verification Plate Cat No 24072800 Service the instrument regularly See Cleaning of the instrument and Maintaining a system log V depending on the laboratory conditions and the use and configuration of the instrument General Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which
55. for example for different controls when 1 sample individually if a different replicate number for the samples are needed When filling the plate with the Add series function note that adding a group of samples of the same type to another location will delete the first group If you want to add the same well type into several separate locations on the plate layout use the Edit function For instructions on how to program the plate layout refer to Programming a plate layout Preprocessing parameters You can define the preprocessing parameters in the Preprocessing window Preprocessing includes two different types of data processing precalculations and kinetic calculations Processing Preprocessing parameters Precalculation Mea Meal Move OK Select Cancel 2 Preprocessing is used with dual two wavelength measurements for example to subtract the reference wavelength data in dual measurements and with kinetic measurements to reduce data in kinetic calculations Table 8 Processing Preprocessing parameters Preprocessing Preprocessing L Pre Calculation L No L Mea1 Mea2 L Meal Mea2 L Meal Mea2 L Meai Mea2 L Mea2 Meat L Mea2 Meat Kinetic ae L Type L No L Average rate L Kinetic rate L Ignore from beginning i L Ignore from end L Maximum rate ee ee Kinetic rate Ignore from beginning a Ignore from enc M rr LOIR L Undefine
56. g a cloth dampened with 7096 ethanol Place the instrument in a large plastic bag Ensure that the lamp and filter wheel chamber cover and measurement chamber door are open and the plate carrier is out Place a cloth soaked in the prepared decontaminant solution into the bag Ensure that the cloth does not come into contact with the instrument Close the bag firmly and leave the instrument in the bag for at least 24 hours Remove the instrument from the bag After decontamination clean the instrument using a mild detergent After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package 68 Adding individual filters to the filter wheel This section explains how to replace a filter in the filter wheel or add an additional filter to the filter wheel Caution Only use filters approved by the instrument manufacturer Cat No 24072800 to verify filters added Caution For certain filters you can use the Thermo Scientific Multiskan Verification Plate Note The filter is a consumable and should be changed regularly owitch the power off 2 Open the lamp and filter wheel chamber cover Caution When handling the filter wheel do not touch any other mechanical or electronic part 3 Lift the filter wheel from the filter wheel slot Figure 8 and Figure 19 Do not touch the filter surfaces Figu
57. he highlighted item 0 9 Use the character keys to enter numerical data and text e and are found under the 1 key The CLEAR C key is used to delete written text or numbers Eoy Use the F1 F3 keys to select the corresponding action from the info text bar Figure 14 The information on the info text bar is updated according to the active menu EH UND 19 Info text bar Protocol 1 Protocol 2 Protocol 3 m mz Ex ER Figure 14 Info text bar of the Multiskan FC user interface Press the FILE key for example for saving the protocol in the Main menu Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol New Open Save Save As Export Export All Protocols Export As Text Import Print Print Protocol Print Results and Delete Use the HELP key for more detailed instructions Press the PLATE in out button to move the plate carrier in or out PLATE in out Press the START button to start reading using the currently selected protocol Press the STOP button to terminate the reading process Data from the plate in progress is lost It can also be used to terminate the possible computer remote control Chapter 5 Menus and Parameters This section describes the menus and the relevant main parameters of the internal software for creating and editing protocols The internal software includes the Main Processing Results a
58. height 15 3 mm Shaker Linear shaking 3 speeds Slow 5 Hz amplitude 15 mm Medium 11 Hz amplitude 3 mm and Fast 20 Hz amplitude 1 mm External printer type HP PCL5 PCL5e or PCL5c 17 Performance specifications Table 16 Photometry This section provides the performance specifications at typical laboratory conditions 22 C relative humidity 40 for the relevant measurement technique and other instrument capabilities Performance specifications Photometry Optical system Quartz halogen lamp Osram 64222 6V 10W interference filter in the filter wheel fiber fiber end optics photodetector signal processing Wavelength range 340 850 nm Filters 8 position filter wheel The instrument is delivered with the following standard filters installed 405 nm 450 nm and 620 nm Additional filters can be ordered separately One silicon photodetector Linearity 0 3 Abs 2 96 well plate 405 nm 0 2 5 Abs 2 384 well plate Absorbance resolution 0 001 Abs Accuracy 1 0 3 Abs or 0 003 Abs which ever is greater 405 nm normal mode Precision CV lt 0 2 0 3 3 Abs 405 nm normal mode Measurement time 7 s 96 well plate fast mode 13 s 96 well plate normal mode 16 s 96 well plate fast mode dual wavelength 13 s 384 well plate fast mode 34 s 384 well plate normal mode 28 s 384 well plate fast mode dual wavelength Long term stability Instrument is
59. in front of the instrument for the plate carrier to come out freely Caution Check that the plate carrier moves in the measurement door closes properly and the lamp and filter wheel chamber cover is closed gt gt 44 3 Press the START button 4 Enter the unknown count with the number keys to Press the START or OK button to accept the selection and to start the 5 o ok EN measurement Note If you want to cancel the run press the F2 key 6 The microplate is measured and the results are automatically calculated according to the predefined protocol Note During the run you can press the STOP button to abort the run 7 Press the F2 key to close the result table and then press the Left arrow key A q twice to return to the Main menu Note The main parameters are locked when a run measured data for this protocol exists Starting a ready made protocol from the list 1 Press the OK button on the Protocol row in the Main menu OR Press the FILE key in the Main menu and select Open using the Down arrow key and then press the OK button Settings Created Modified 05 2008 13 00 14 17 05 2008 13 01 15 Clamydia 17 06 2008 13 02 02 17 06 2008 13 02 02 Demo1 17 06 2008 13 02 34 17 05 2008 13 02 34 Demo2 17 06 2008 13 02 50 17 06 2008 13 02 50 17 05 2008 13 00 14 17 06 2008 13 0115 0 1 0 0 Demo3 17 06 2008 13 03 02 18 06 2008 09 28 14 1 0 0 0 0 Exampl
60. inish Disposal of materials Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Refer to local regulations for the disposal of infectious material 67 Warning The samples can be potentially infectious Dispose of all used plates disposable gloves syringes disposable tips and so on as biohazardous waste Be cautious and always use gloves Decontamination procedure If you have spilled infectious agents carry out the decontamination procedure Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed It is strongly recommended to perform the complete decontamination procedure before relocating the instrument from one laboratory to another Example of decontaminants a Xx TN c Ethanol 70 Virkon solution 1 396 Glutaraldehyde solution 496 Chloramine T Microcide SQ 1 64 Decon 90 min 4 to use formaldehyde since even small traces of formaldehyde negatively affect the enzyme Caution If local or laboratory regulations prescribe regular decontamination it is not advisable being used in EIA tests resulting in inconsistent test results Prepare the decontaminant Empty the plate carrier Ensure that you are wearing disposable gloves owitch off the power and disconnect the power supply cable Disinfect the outside of the instrument or to remove stains usin
61. ion For PC software related issues refer to the Thermo Scientific Skanlt V Software for Microplate Readers User Manual Cat No N16243 or Skanlt Software for Microplate Readers Technical Manual Cat No N16046 For the latest information on products and services visit our websites at http www thermoscientific com http www thermoscientific com platereaders http www unitylabservices com In an effort to produce useful and appropriate documentation we appreciate your comments on this user manual to your local Thermo Fisher Scientific representative Manufacturer Thermo Fisher Scientific Oy Ratastie 2 P O Box 100 Fl 01621 FINLAND Safety symbols and markings These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated Safety symbols and markings used on the Multiskan FC The following symbols and markings appear on the type label and the instrument itself Power ON Power OFF Warning The plate carrier may come out risk of operational failure or damage Warning Hot plate risk of burns Serial number Catalog number Date of manufacture Consult instructions for use WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2012 19 EC 4c lit ee O A black label with the following text Figure 11 CAUTION WARNING DISCONNECT SUPPLY BEFORE
62. ising out of the use or inability to use this product Power failure The system requires uninterrupted power supply in order to operate correctly Thermo Fisher Scientific has no responsibility whatsoever for system malfunctions arising from power failures Warranty The Thermo Scientific Microplate Instrumentation products are fully guaranteed against defective parts and materials including defects caused by poor workmanship for a period of one year from the date of delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with instructions from Thermo Fisher Scientific The warranty is invalid if products have been misused or abused For the warranty to be effective the product must have been purchased either directly from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject to the following exclusions e Any defects caused by normal wear and tear e Defects caused by fire lightning flood earthquake explosion sabotage war riot or any other occurrence of the character listed above e Refurbished products that are subject to different warranty conditions THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSE
63. kvE aa ug xeck a vedo raa arp aud oeg dues 13 Connecting the power supply cable seesssssssssesseeeen nennen nennen nennen nnn nnns 14 GONMECIMG to T COMD UN CL cai c2 atest cok ai ciea alee itieteiaa ai Steieet eect dist iaaeatateat aie acacia atc acuaendteate 14 Connecting to Ax DUM Gl m E E D T 14 S147 G1 011A o 1 ET Ere 15 Performing the operational check eesssessssssessseesseeseee eene nennen nennen nn re nnne nenne nennen ns 15 Settings after IBSIalIatlQr cete i tete Ert tate ape Ove v o Sume ed xd tust Ead tects tiene Desc es usui p 15 Installation of Skanlt Software for Microplate Readers esses 15 Chapter 3 Multiskan FC Main Parts iunior oue dace e voi ga aua du C wcv Eo us vae vada Cua vao ga usw dvo x v vkE mnnn 16 FION VIG T 16 BAK QU cres Tt ee ee eee er eee fe ee 16 ILS MIC WV asp he s cast rina 17 USB MENON device POM TREE 17 MECUD ALON sss ig Uem 18 SUNL e ES Eo MEE M NUM UU ee 18 Jeder secisiolg mc ND 18 Chapter 4 Operating the Internal Software 11 lese eeee Lees eese eene enne nnn nnns 18 Display and keys for NAVIGATING and editing csse eene nnn nnn nnn nns 18 ETE T T AE 19 Chapter 5 Menus and Parameters oi euo uueiua E exo sa acu sexu Ead ob Rack cunc ix E dao da
64. l room for cables COVers and so on e there is sufficiently room behind the instrument to enable disconnecting the device e there is enough clearance in front of the instrument for the plate carrier to come out freely e the ambient air is clean and free of corrosive vapors smoke and dust e the ambient temperature range is between 10 C 50 F and 40 C 104 F e the humidity is low so that condensing does not occur relative humidity is between 10 and 8096 non condensing Caution Do not operate the instrument in an environment where potentially damaging liquids or gases are present Setups Releasing the transport lock Caution Make sure the transport lock has been released before you put the instrument into operation 1 Open the lamp and filter wheel chamber cover Figure 3 Figure 3 Transport lock inside the instrument and transport lock tag 2 Unfasten the transport lock bar counterclockwise using the Allen key supplied Figure 4 11 Figure 4 Unfastening the transport lock 3 Pull the transport lock bar out Figure 5 Figure 5 Pulling the transport lock out 4 Keep the transport lock bar and tag Figure 6 for future relocation or transportation of the instrument 12 Figure 6 Transport lock bar and tag removed Installing the filter wheel 1 Unpack the filter wheel Figure 7 It is delivered in a filter wheel box Check that all filters are clean and undamaged Position sl
65. ls L EE Wells Measurement NENNEN Filter 1 L No filter L 405 1 L 450 2 L 620 3 Filter 2 n DE L No filter L 405 1 L 450 2 L 620 3 ee L Fast L _ L No of readings 1 100 E ege tt L No L Before measurement L Background Wwe s 7 Temperature C ee 21 Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol Protocol The Protocol row in the Main menu shows the name of the active protocol You can open another protocol by pressing the OK button on the Protocol row or by pressing the FILE key The list of protocols saved in the internal software will appear You can also open a particular run of the protocol in question protocol with measured data with the Menu function Also the runtime is shown on the Protocol row For more details on opening a protocol refer to Opening a new protocol i Main Processing Results Settings Protocol Created Modified 06 2006 13 00 14 17 5 20808 13 00 14 17 06 2008 13 01 16 17 06 2008 13 01 15 17 06 2008 13 02 02 17 06 2008 13 02 02 17 06 2008 13 02 34 17 06 2008 13 02 34 17 06 2008 13 02 50 17 06 2008 13 02 50 17 06 2008 13 03 02 18 06 2008 09 28 14 Endotoxin 17 06 2008 13 03 40 17 06 2008 13 03 40 Helicobacter 17 06 2008 13 04 44 17 05 2008 13 04 44 HI 1 2 17 06 2008 13 05 30 17 06 2008 13 05 30 MTT 17 06 2008 13 05 54 17 06 2008 13 05 54 Mo
66. measurement mode The Fast mode produces fast results with a slightly narrower linear absorbance range than with the Normal mode Number of readings sets the number of measurements per well One means that each well is measured only once and two or more means that the measurement is repeated kinetic The number of readings can be anything between 1 and 100 If the Number of readings is higher than 1 the measurement is considered kinetic and the interval time between the readings must be set Kinetic calculations can be added to the protocol For instructions on how to program a wavelength for example refer to Programming a wavelength Shaking parameters You can define the shaking parameters in the Shaking window shaking paramet No Before measurement Background Move OK Select Cancel You can select between no shaking shaking before measurement and background shaking The shaking speed can be set to Slow Medium or Fast Refer to Table 1 Background shaking is active during the intervals of kinetic measurements The shaker shakes in sections of 1 second with the slow speed For instructions on how to program shaking refer to Programming shaking 24 Incubation parameters Note Incubation is only available if the instrument configuration supports incubation You can define the incubation parameters incubation time and temperature in the Incubation window Incubation parameters Temperature C
67. my 24072800 Multiskan Verification Plate IOPQDOCEN08265 Multiskan FC IQ OQ PQ English 84 Appendix A System Log Instrument name number Appendix B Certificate of Decontamination Name Address Tel Fax Instrument Serial No A confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place oignature Name block capitals Please include decontaminating solution used 86 Glossary absorbance optical density decontamination disinfection EIA ELISA error message fast mode filter initialization tests kinetic measurement normal mode optical density absorbance photometer photometry self tests single measurement transmittance USB Index A logarithmic function of the transmission of a wavelength of light through a liquid log Ml dime
68. nd Settings menus The menu tree is displayed in Table 2 Table 2 Menu tree Main Procesing Resuts Setings Plate layout Plate format Preprocessing Calculated results Filters L Calculation L Calibration curve L Shaking Interpretation Quality control Startup Incubation L Quality control Results in list format DE S S Main menu You can specify the instrument related parameters in the Main menu The Main menu contains the Protocol Plate format Measurement Shaking and Incubation parameters 20 Processing Results Settings Protocol Untitled t3 Plate format 95 Wells amp Measurement 405nm 1 reading V Shaking No 370 C Incubation No Move OK Edit Demol Demo2 Demo3 4 info text bar The clock on the Main tab shows the real time The Incubation icon also shows the current temperature only Multiskan FC with incubator model See the info text bar for the required actions of the F1 F3 keys The action text on the info text bar changes according to the level of the internal software protocol exists After the measurement the plate format measurement shaking and incubation parameters cannot be changed For more information refer to Chapter 6 Starting Ready made Protocols Note The parameters of the Main menu are locked when the run measured data for the Table 3 Main parameters L Protocol Created Modified Runs L 96 Wel
69. nsion Abs Removal or neutralization of radiologic bacteriological chemical or other contamination The destruction of pathogenic bacteria usually with an antiseptic chemical or disinfectant Enzyme immunoassay An immunoassay using a color changing enzyme substrate system for indicating results A diagnostic test method to measure or detect a substance using antibody antigen reactions Abbreviation for enzyme linked immunosorbent assay Indication that an error has been detected The detector moves over the well without stopping An optical component to select a wavelength So called self tests which are carried out before operation to ascertain that the necessary instrument adjustments have been carried out Measurement over a time period at certain intervals The detector stops over the measured well log 1 transmittance log Mlo dimension O D A device measuring absorbance or optical density The measurement of the properties of light particularly luminous intensity Initialization tests which the instrument performs before operation Synonym to endpoint measurement assay method The measurement is only done with a single reading The ratio of transmitted and incident light o Mo Universal serial bus Use the Find option for searching words information in this user manual 87
70. ntroducing a filter to the internal software This section provides an example on how to enter the information of an additional filter which has been added to the filter wheel Note Before introducing the additional filter to the internal software make sure the filter is 1 physically inserted into the next free filter position in the filter wheel and the filter wheel is placed into the filter wheel slot 1 In the Main menu select the Settings menu using the Left arrow Key 2 Select the Filters row using the Down arrow key and press the OK button vi 63 fi settings Filter settings Wavelength nm 405 Comment OK Edit 4 Prev P Next Cancel Remove Select the empty filter position by pressing the Right arrow key until the filter wheel on the display rotates to the empty position and the text Empty is shown Press the OK button Enter the wavelength of the filter for example 492 with the number keys and then press the OK bution Press the F1 key to accept the filter settings Note The instrument starts the filter initialization 6 Press the Right arrow key to return to the Main menu Removing a filter You can remove a filter from the instrument settings by selecting the position and pressing the F3 Remove key Note The filter must physically be removed from the filter wheel Printer You can add a header in the Printer parameter window for example the laboratory informa
71. o public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply zm Note 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation mm Chapter 15 Troubleshooting Guide Note Do not use the instrument if it appears that it does not function properly B Error and warning codes When an error is detected the current operation is terminated After an error it is best to abort the current run and restart from the beginning after the problem is fixed The error Table 18 and warning codes Table 19 that may appear in Skanlt Software for Microplate Readers are presented below 79 Table 18 Error codes reported ST 0 Thecommand was executed successfully it received the PC software vendor not valid the PC software vendor 4 Plate positioning error Check that there is nothing preventing free plate Contact service 5 Measure head positioning error Check that there is nothing preventing free measure head movement Contact service eam aa Contact service 8 AD converter noise level is too high Contactsevce 1 1 1 11 Lamp failure Replace the lamp ap MN It is also p
72. ossible to get this error if either one of the measure head or plate motors has lost steps so that the position is wrong Attempt to set the instrument serial number Do not try to set the instrument serial number when it already has been set 12 Not enough memory for a new user defined The internal memory is full Take a backup by parameter exporting the protocols runs Refer to Chapter 9 Printing Exporting and Importing Release space from the internal memory by deleting protocols runs from the internal memory 13 ouch an error during startup which prevents Restart the instrument measurement If you are unable to eliminate the error contact service The distance between plate wells is too short Ensure that the well distance in the PC software for scan measurement plate template is large enough or use Normal measurement mode The sampling time for a single result is too long for scan measurement The plate cannot be moved as slowly as the sampling time requires Failed to adjust a suitable lamp intensity for a filter positions If the error persists contact service 14 5 1 16 7 Temperature is not changing Use a at least a 4 C higher incubation temperature than the environmental temperature This applies to both the protocol and startup temperature 1 Too high dark signal level The dark level is This error may also be caused by a measure head ul checked once for each measured plate or plate position erro
73. ot TS3580 Figure 7 Multiskan FC filter wheel i Caution Do not touch the filters with your bare hands Caution When installing the filter wheel do not touch any other mechanical or electronic part 2 Place the filter wheel into the filter slot so that the filter wheel numbers 1 8 face outwards Figure 8 A magnet locking mechanism will automatically lock the wheel in the correct position and the optical filter position sensor will make sure the correct filter is used during measurement 13 Figure 8 Placing the Multiskan FC filter wheel into the filter slot If the filter wheel seems to be jumping in the filter wheel slot you have inserted the wheel incorrectly and the magnet is rejecting it Turn the wheel over 3 Close the lamp and filter wheel chamber cover Connecting the power supply cable Warning Do not operate your instrument from a power outlet that has no ground connection Do not use a power supply cable other than the Thermo Scientific power supply cable designed for your region 1 Ensure that the ON OFF switch Figure 9 at the back of the instrument is in the OFF O position Connect the power supply cable to the power supply connector and plug in the instrument Connect the power supply to a correctly installed line power outlet with a grounded conductor Figure 9 Power supply connector USB port for printer USB port for PC USB port for memory device Figure 9 Connecting the power s
74. ports FE The kinetic timing is relative This means that each time point is calculated from the first measurement point with the filter You can print or export the selected data For further information refer to Chapter 8 Viewing Results Calibration curve The calibration curve is shown in the Calibration curve window Results 0 OK Edit The report also includes the calculated fit parameters of the curve 41 You can use both the curve on the plate and a previously created calibration curve of the same assay protocol You can store a curve for future use with the FILE function You can load a saved curve for the active protocol with the FILE function If a stored curve is in use for calculation the calibrators measured with the current plate can be taken into use with the Menu function Note The plate layout must include calibrators and corresponding calibrator parameters such as concentrations to enable them for further calculations for example curve fits Refer to Curve fits and Programming a plate layout Note Verify the correctness of the calibration curve EE You can disable and enable calibrator replicates from the curve by pressing the OK button in the Calibration curve window If the user enables automatically disabled data the user is responsible for data quality You can print or export the selected data For further information refer to Chapter 8 Viewing Results Qu
75. r Warning If the instrument has been in use and you need to replace a burned lamp the lamp and its surroundings may be very hot Wait for the lamp to cool down before replacing it Caution When handling the lamp do not touch any other mechanical or electronic part 3 Turn aside the two clamps which keep the lamp in place Figure 24 and Figure 25 Lift the lamp up with the terminal socket 5 Loosen the two screws of the terminal socket and pull the lamp from the terminal socket Figure 24 Changing the lamp 6 Refit the terminal socket to the contacts of the new lamp approved by the manufacturer Osram 64222 6V 10W 7 Tighten the screws of the terminal socket and put the new lamp in place 72 Match the notch of the lamp with the alignment tab LE cene c E ne d wirt Le ag Stites etn Figure 25 Clamps open Note Place the lamp and terminal socket into the correct position Refer to Figure 25 8 While holding the lamp in place turn the two clamps to lock the lamp into position Figure 26 Figure 26 Lamp changed and clamps closed 9 Close the lamp and filter wheel chamber cover 10 Plug in the instrument and switch the power on 73 Refitting the transport lock When you relocate the instrument or ship it for service make sure you refit the transport lock 1 The instrument has to be powered on 2 Press the PLATE in out button so that the plate carrier goes in PLATE in out 3
76. r see errors 4 and 5 Contact row column service Contact service working firmware is corrupted XY table position calibration failed This may be reported after startup 24 Temperature reference is out of limits Contact service ul Incubation is not available if this error is uu RN reported 80 Contact service One or more heaters not ok Contact service Filter 1 not selected in measure parameters Select filter 1 Filter information in the protocol does not match Edit the protocol or add the correct filter to the filter the filter wheel wheel EE 29 50 s 53 The file already exists Save with another file name a 56 29 50 51 52 53 54 55 56 of 08 File open error File not found The media is full First take a backup and then delete some protocols or runs If Defragmenting file system messages start to appear on the display you should already at this stage make more space by deleting some protocols or runs 57 Otherfileeror noneofthe previous Contactservce 1 1 1 1 58 _ Firmware update aborted by user o o O 60 The printer is out of paper 1 1 Addpapertotheprinter Table 19 Warning codes reported Unable to comply with the defined kinetic The minimum timing depends on the combination of interval measure parameters plate movement parameters number of wavelengths and number of measured wells 101 Th
77. re 19 Removing the filter wheel 4 Remove the filter spring by unscrewing the four 4 spring position holding screws Figure 20 and Figure 21 Caution Do not touch the filter glass surfaces with your bare hands Caution The magnet in the middle of the filter wheel attracts both the screwdriver and the screws Make sure the screwdriver or the screws do not scratch the filters 69 Figure 20 Removing the spring position holding screws Filter spring Filter Filter wheel Next free position for additional filter ra 7T Spring position holding screws Figure 21 Filter wheel parts Figure 22 Individual filter showing the arrow 70 5 Note Arrow pointing up Figure 23 Inserting a filter into the next free position in the filter wheel Insert a new filter or an additional optional filter Figure 22 into the next free position in the filter wheel Figure 23 When inserting the filter the arrow must be pointing upwards as in Figure 23 The wavelength of the filter is marked on the filter package as well as on the filter side that is the three digits before the last digit of the number sequence xxxxx NNNx where NNN is the wavelength The filter positions are marked on the other side of the filter wheel The filter wheel has at present three filters factory installed Table 14 Note Do not change the positions of the factory or earlier installed filters Insert the optional at filters into filter
78. rotocol row in the Main menu ox o Press the FILE key and select Open using the Down arrow key and then WV ok press the OK button oelect the protocol you want to export from the protocol list using the Up or 3 A wv Down arrow key 60 ff Main Protocol Created Move OK Select Cancel Press the FILE key and select Export using the Down arrow key Then press the OK button If you also want to export the runs that are created measured with the protocol press the OK button If not press the Right arrow key and then the OK button Protocol information will be exported mem Press the F2 key to close the protocol list and return to the Main menu the protocol name is stored in file mapping fc Do not delete the mapping file Note When a protocol is exported to the USB memory device the data is saved in one file and Importing a protocol 1 2 a V E ove AVE Insert the USB memory device into the USB memory device port of the instrument Press the OK button on the Protocol row in the Main menu OR Press the FILE key in the Main menu and select Open using the Down arrow key Then press the OK button Press the FILE key and select mport using the Down arrow key and then press the OK button Select the protocol you want to import from the USB protocol list using the Up or Down arrow key and then press the OK bution also import the protocol to an ins
79. t searches for the absolute maximum rate If the highest absolute rate value is increasing it is shown as a positive number If the maximum rate is decreasing it will be u J presented with the prefix e Increasing The software searches for the maximum increasing rate The rate is shown as a positive number If no increasing rate is found the result is NaN not a number e Decreasing The software searches for the maximum decreasing rate The rate is shown as a positive number If no decreasing rate is found the result is NaN not a number e Kinetic rate Select to view the results in either seconds s or minutes min The kinetic rate is always calculated per second but if you select per minute the time is automatically converted to minutes after the calculations e Ignore from beginning Ignores a defined number of points readings counted from the first reading e Ignore from end Ignores a defined number of points readings counted from the last reading e Window Select the number of consecutive readings to use for evaluation The highest reaction rate for each well is calculated using a sliding window A window defines how many measurement points are included in the measurement calculations The size of this window is given in the Window parameter box For example if the number of measurements is ten and the Window parameter is three the system will calculate the first rate using the me
80. they should be applied Always ensure that the electrical supply in the laboratory conforms to that specified on the type label of the instrument To guarantee the continuous reliability and accuracy of the Multiskan FC avoid disturbing any of the optical system components A misalignment of the light path affects measurements e Keep the filters clean to ensure proper function and accurate results e Prevent any liquid from entering the instrument e Keep the instrument free of dust and other foreign matter e Avoid touching the lens surfaces filters or detectors with your bare hands e Perform the operational check regularly see Performing the operational check In the event of any damage contact your local Thermo Fisher Scientific representative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance see Cleaning of the instrument 66 Clean the keypad and display surface with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used Caution Do not autoclave any part of this instrument Immediate Although the Multiskan FC is constructed from high quality materials you must imme
81. tion to all of the reports to be printed You can do this by selecting Yes for the Enable header The maximum length of the header is about 50 characters Startup In the Startup parameters the Plate position setting defines the plate carrier position after the instrument is switched on You can set the startup temperature The instrument starts to warm up to the set temperature immediately after it has been switched on 64 on The temperature set in the protocol overrules the startup temperature If the protocol does Note The set startup temperature is only activated when the instrument is powered switched not include incubation the startup temperature remains Chapter 12 Emergency Situations Handling emergency situations In case there is any abnormal situation during the operation such as fluids spilling inside the instrument 1 2 3 4 Switch off the instrument Figure 9 Unplug the instrument immediately from the power supply Figure 9 Carry out appropriate corrective measures However do not disassemble the instrument If the corrective measures taken do not help contact authorized technical service or your local Thermo Fisher Scientific representative Chapter 13 Maintenance Regular and preventative maintenance Contact local authorized technical service or your local Thermo Fisher Scientific representative for assistance if necessary Maintenance checklist This chapter contains an outline o
82. to or from an instrument to another of the same type When exporting a protocol it is also possible to include measurement data in the exported file This feature can be used as a backup for the measurement data Note It is highly recommended to take a database backup before deleting runs Taking a 1 backup is the responsibility of the user It is only possible to import measurement data to the same instrument as it was generated from Before running the imported protocol check that the filter and filter position to be used are correct The calculated results or stored protocols can be printed after the measurement using the FILE key The printout is sent to the external printer according to the general settings of the instrument The printed data can be selected The report to be printed can be defined by FILE Print Define Report in the main level of Results Each active data table can also be defined by using FILE Print 58 Results data is expressed as when available space is too little Note It is recommended to format the USB memory device if export or import fails Printing or exporting data This section provides information on how the active run data measurement results can be printed or exported to the USB memory device 1 If you are exporting data insert the USB memory device into the USB memory device port of the instrument OR If you are printing data ensure that the printer is connected and switche
83. trument with the same configuration Note You can only import the runs to the same instrument they were created with You can Note It is recommended to format the USB memory device if export fails For more information 1 refer to Chapter 15 Troubleshooting Guide O Press the F2 key to close the protocol list and return to the Main menu 61 Chapter 10 Shutdown 1 Remove any plates still in the instrument pa Press the PLATE in out button Make sure the plate carriage goes inside the in out instrument and check that the measurement chamber door closes properly 3 Switch off the instrument If you have spilt infectious agents on the instrument disinfect with 70 ethanol or some other disinfectant see Decontamination procedure Chapter 11 Modifying Settings You can modify settings related to the system filters printer or startup in the Settings menu Processing Results fi Settings Sern Sustem a Printer Startup OK Edit Demol Demo2 Demo3 System settings The Multiskan FC instrument s internal software version serial number and model are shown in the System settings window Model 1 refers to Multiskan FC and Model 2 to Multiskan FC with incubator You can set the date date and time format and language in the wv ok System settings parameter window Select the item to be changed with the Up and Down arrow keys and press the OK button Setting the date
84. tungsten halogen lamp as the light source Figure 2 Wavelength selection is made by means of interference filters Detector and preamplifier Background reading point ud Light source Opening for blank reading 9 X Filter Figure 2 Optical system The selected wavelength is directed at a moving measurement head by an optic fiber The measurement head moves to select the well column and the track to select the row During measurement the light from the lamp passes through an interference filter to an optic fiber The filter selects from the lamp spectrum the exact wavelength desired The optic fiber directs the light below the selected well of the plate After passing through the well the light is sensed by a photodetector in the measurement head above the well Blank air and dark level measurements are needed to calculate the absorbances The Multiskan FC performs these measurements once at each measured row The blank is measured from an opening at the left end of the plate tray and the dark signal when the plate tray edge opposite the blank hole blocks the light path Chapter 2 Installation Warning The Multiskan FC weighs 8 5 kg 18 7 lbs and care must be taken when lifting it Unpacking How to unpack Move the packed instrument to its site of operation To prevent condensation the instrument should be left in its protective plastic wrapping until the ambient temperature has been reached Unpack th
85. um rate is selected the software searches the data for the maximum rate found in each well To obtain the maximum rate a series of linear curve fits will be performed for different segments of the measurement value absorbance vs time curve Figure 15 The first segment starts at the first data point within the selected time and measurement range the second segment starts at the second data point and so on until all the data points have been analyzed All the rate calculations are evaluated to determine the maximum rate In other words the LLS fit of m span points are sequentially fitted through each of the n data points There will be n m 1 curves produced from this You can specify the number of data points in a segment with the Window setting 30 1 4 1 2 1 08 Max Rate p 0 6 0 4 0 2 Abs V 2 4 b g 10 Ce Time s Figure 15 Determining the maximum rate with window value 2 You can define the following settings Processing Preprocessing kinetic param Reaction Undefined Kinetic rate Ignore from beginning Ignore from end Window 24h Move OK Edit e Reaction Define whether the reaction is supposed to produce increasing or decreasing signal levels There are three options for this setting e Undefined default The reaction can be increasing decreasing or both for example first increase and then decrease that is peaking The software does not produce any warnings in either case I
86. upply cable Connecting to a computer If you are using a computer with the Multiskan FC connect the communication cable to the USB port for the computer Figure 9 Connecting to a printer If you are using a printer connect it to the USB port for the printer Figure 9 The external USB printer protocol that can be used is HP PCL5 PCL5e or PCL5c 14 Switching on Check that all the cables are properly fitted according to the installation instructions Switch the instrument ON plug unplug the printer cable during Importing Exporting data from to the USB memory device Caution 1 Do not switch the power off during Performing self diagnostics 2 Do not 3 Do not plug unplug the USB memory device during Printing or Performing self diagnostics Performing the operational check Before you take the instrument into use make time to perform the following operational check 1 Switch the instrument ON 2 Check that the plate carrier comes out from the instrument and the Self diagnostics passed text is shown for a while on the display 3 Press the START button twice 4 Check that the plate carrier moves in and the measurement chamber door closes properly The instrument starts the measurement After the measurement the plate carrier comes out No error messages should appear on the display mwewm 2 Press the F2 key to close the result table 6 Press the Left arrow key twice to return to the Main menu
87. ve OK Load The maximum number of protocols is 99 Table 4 Protocol structure A measured run of a protocol includes run data and protocol information for that run You can start the measurement of an active protocol with the name Untitled but the results of an Untitled protocol are not saved unless you save the active protocol including the measured run data with a new name 22 Note It is recommended to back up the protocols regularly The backup is created by exporting the protocols to the USB memory device Note If you make any changes to the processing parameters of a measured run in the Processing menu the changes are made to that run only If you want to make changes to the protocol and thereby to the future runs you must open the protocol make the changes and press the FILE key Save to save the changes EJ Note When you press the START button the software always loads the saved protocol pi regardless of what parameters are active on the display For instructions on how to start and create protocols refer to Chapter 6 Starting Ready made Protocols and Chapter 7 Protocol Creation Plate format parameters You can select the plate format 96 or 384 in the Plate format window Note The 384 format is only available if the instrument configuration supports 384 well plate measurements E Main Processing Results Settings Protocol Untitled Plate format 96 Wells Measurement 4
88. velength range of 340 to 850 nm The instrument allows shaking The instrument is controlled through the built in graphical user interface and keypad Multiskan FC can be connected to the Skanlt Software The Multiskan FC is available in the following configurations e Multiskan FC e 96 well plate reading with internal shaking e Multiskan FC with incubator e 96 and 384 well plate reading with shaking and incubation Figure 1 Multiskan FC microplate photometer Intended use The Multiskan FC is a microplate photometer for measuring absorbance from suitable microplates and strips in 96 well plate formats and optionally microplates in 384 well plate formats that meet the ANSI SBS standards The Multiskan FC as standalone instrument or with Skanlt Software can be used in research or routine test laboratories by professional personnel The Multiskan FC is part of the analyzing system used by the end user who therefore is responsible for validation of the whole system for reliable and safe results If the assay performance is essential for the analysis the test result has to be ensured with internal quality controls or with an alternative test It is recommended to use Good Laboratory Practice GLP during the analyzing process Principle of operation The Multiskan FC is a filter based microplate photometer Measurement is vertical The instrument reads from bottom to top The optical system is based on one channel optics with a
89. ver it is likely that the protection has been impaired the instrument should be made inoperative and be secured against any unintended operation Contact authorized technical service immediately The protection is likely to be impaired if for example the instrument e Shows any visible damage e Fails to perform the intended functions e Has been subjected to prolonged storage under unfavorable conditions e Has been subjected to severe transport stresses 82 Chapter 16 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information List of filters Table 20 Codes for filters Code List of spare parts and accessories Table 21 Codes for spare parts and accessories Co em Quy Y 5187139 Skanlt Software for Microplate Readers Research Edition CD 5187149 Skanlt Software for Microplate Readers Drug Discovery Edition CD 1 1410101 Halogen lamp 6V 10W BEES N04001 USB A B device cable MEO 83 List of Thermo Scientific plates Table 22 Codes for plates Code immulon Plates 96 Well o O Micirr 96WellPlates O o Microtiter384 Well Plates ImmulonStrip Assemblies in frames ImmulonStips Microtiter Breakable Strip Assemblies in frames Microtiter Solid Strip Assemblies in frames Verification tools Table 23 Codes for verification tools Cos Mm Qu
90. w key and press the OK button The limit editor is opened Processing Interpretation parameters 4 gt Move OK Edit Accept Cancel 7 Select Sample and press the OK buiton 8 Select CTRL 1 by using the Down arrow key and press the OK button 9 Select Raw and press the OK button 54 10 Select Operator using the Right arrow key and press the OK button 11 Select and press the OK button 12 Select Constant using the Right arrow key and press the OK button 13 Enter the constant for example 0 050 using the number keys and press the OK button 14 Press the F1 key to accept the formula 15 Select Interpretation 2 using the Up arrow key and press the OK buiton 16 Enter the category name for example Pos positive using the letter keys and then press the OK button 17 Press the F1 key to accept the interpretation Processing Interpretation parameters Interpretation 3 Limit 2 Interpretation 2 Limit 1 Raw CTRL1 0 050 Interpretation 1 Neg Source matrix Move OK Edit Accept Cancel Adding a QC rule to the protocol The rules are normally given in the kit insert and can read for example the absorbance of Blank has to be smaller than 0 100 Abs In this case the rules to be entered would be Sample Raw BLA Operator Constant 0 100 The following example shows how to add a Quality Control QC rule to a protocol Processing a Quality control
91. wheel positions after the earlier installed filters Table 14 Example of the filters in the filter wheel positions 1 8 Filter wheel position Wavelength nm a a 405 example of factory installed filter 450 example of factory installed filter 620 example of factory installed filter a 10 11 Write down in Table 14 the additional filters you have installed into the filter wheel It is recommended to add the filters in ascending wavelength order Place the filter spring into its original position and fasten the four screws symmetrically Slide the filter wheel back into the filter wheel slot with the filter numbers 1 8 facing outwards Figure 8 The magnet locking mechanism will automatically lock the wheel in the correct position Refer to Installing the filter wheel Close the lamp and filter wheel chamber cover Switch the instrument on Add the filters to the internal software s general instrument parameters These parameters are set in the Settings menu Refer to Settings menu and Table 14 71 amp Note The measurement results will be incorrect if the filter parameters differ from the actual Bt filters in the filter wheel Now the filters are ready for use Changing the lamp Caution Only use the lamp approved by the supplier Cat No 1410101 Lamp quartz halogen lamp Osram 64222 6V 10W Switch the power off and unplug the instrument 2 Open the lamp and filter wneel chamber cove
92. wns You can save the curve from the curve window using FILE Save calibrators and load it using FILE Load curve 36 Note The stored curve must be older than the measured run data This means that if a new curve is stored the earlier saved assay run data cannot be recalculated against the new curve Interpretation parameters You can define the qualitative calculation classification in the Interpretation window Processing esie Settings Plate layout Preprocessing Calculation QC Quality control Move OK Edit Demo Demo2 Demo3 Processing Interpretation L Source data L Interpretation 1 L Limit 1 L BLA L Raw Blanked Dual Kinetic Concentration L CAL1 CAL1 2 L CTRL1 CTRL5 L Raw Blanked Dual Kinetic Concentration L L Raw Blanked Dual Kinetic Concentration SS LConsan o t10 Trw SD CVX O o epeete y pt interpretation o oo L Enter class in free text field Oooo eens S S The pop up menu appears when you press OK Depending on the level of the internal software the FILE key opens a list of actions possible for the current protocol Interpretation classification can be used in a qualitative assay a run where no absolute concentration is calculated and it can also be used for a quantitative assay a run when the interpretation is made based on the calculated concentration Figure 17 In a qualitative classification an

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