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Operating and Maintenance Manual 322375H01

1. Separation distance according to frequency of transmitter m Rated maximum output power of 150 kHz to 80 MHz 80 MHz to 800 MHz 800 MHz to 2 5 GHz transmitter W 1 2 d 1 2VP d 2 3VP 0 01 0 12 0 12 0 23 0 1 0 38 0 38 0 73 1 1 2 1 2 2 3 10 3 8 3 8 7 3 100 12 12 23 For transmitters rated at maximum output power not listed above the recommended separation distance meters m can be estimated using the equation applicable to the frequency of the transmitter where P is the maximum output power rating of the transmitter in watts W according to the transmitter manufacturer NOTE 1 At 80 MHz and 800 MHz the separation distance for the higher frequency range applies NOTE 2 These guidelines may not apply in all situations Electromagnetic propagation is affected by absorption and reflection from structures objects and people NOTE Medical Electrical Equipment needs special precautions regarding EMC and needs to be installed and put into service according to the EMC information provided in this manual NOTE Portable and mobile RF communications equipment can affect Medical Electrical Equipment NOTE The use of accessories transducers and cables other than those specified with the exception of transducer and cables sold by the manufacturer of this device as replacement parts for internal components may result in increased emissions or decreased immunity of the Controller NOTE The
2. Event Code Description AM Alarm Mute AH Temp A High Alarm AL Temp A Low Alarm BB Running on battery power BH Temp B High Alarm BL Temp B Low Alarm BP Bypass Sensor Open BV Low Battery Voltage Alarm BY Hot Gas Bypassing CA Temp A Calibration Alarm CB Temp B Calibration Alarm CG Bypass Sensor Calibration Alarm F Filling FD Fill Disabled FT Fill Time Alarm HG Hot Gas Bypass Time Alarm LH High Level Alarm LL Low Level Alarm LO Lid Open Alarm PF Power Failure SC Stuck Closed Alarm 0 Stuck Open Alarm US Liquid Usage Alarm UW Usage Warning ZO Level Zeroing Once the event log has been downloaded it can be plotted to facilitate analysis 7 0 Fill St ill Stop D O nnn enn ne one nn nn 2 no 2 one 2 5 6 0 40 0 4 Ez F 7 p 1 38 F N8 4 50 LN2 supply exhuasted 40 80 0 4 1 4 n Slope of LN2 Level 5 Fill Start indicates liquid usage lag 1200 fF 4 2 0 dan oe eene eee woe EEA Bocce cae i Storage space begins to T 1 0 warm LN2 is completely depleted 200 0 0 0 o 9 S S e S S e F3 afr i rd s Y E Date Time TEMP 4 TEMP BTM LN2 LEVEL Figure 10 Sample Plotted Ev
3. Figure 19 Standard reverse RJ 45 network cable configuration Numbering as viewed from side of connector opposite latch Note Pressing STOP FILL will delay the automatic fill circuit for 30 minutes To initiate the Automatic Fill Circuit restart controller 91 of 133 326636H01 Rev 7 4 3 Printer Setup The Controller compatible printer kit allows users to print a hard copy of the freezer s status at a set interval and as events occur The Controller will print the current status of the freezer in the event log format The default print interval is 30 minutes NOTE Adjusting the print interval will not affect the event log interval These two parameters are independent The event log interval can be adjusted using ASCII commands All printed events are not necessarily logged in the event log however all the events in the event log are printed Controller Printer Kit P N 11544943 e Epson LX 300 II Serial Dot Matrix Printer e RS 485 Converter and Adapters e Users Guide Installation 1 Setup printer as described in the included user s guide 2 Assemble and connect RS 485 converter and adapters as shown below 3 Connect the printer to Controller serial port 1 or 2 via the RS 485 converter assembly 4 Set the corresponding COM Setup to 9600 N81 5 Set the corresponding COM Type to Printer 6 Adjust the Print Interval to the desired value 7 Test setup by forcing an event or printing a new header or event
4. High Level Al The display will read High Level Alarm UC ENTE 8 0 in 5 Press SETUP Press ENTER for Until the display reads Advanced Level Menus Advanced Level menus 6 Press ENTER The display will read Auto Fill Control Use the keys to adjust and press ENTER to save the change ENABLED Auto Fill Control 45 of 133 7 2 2 3 Level Offset 326636H01 RevA Since the annular line that carries the pressure level signal to the Controller is not located at the very bottom of the LN2 column a Level Offset is needed This value is determined and set at the factory and should not be adjusted to fix an inaccurate level reading unless setting Startup Procedure or if instructed to do so by an authorized CryoExtra For liquid level calibration see section 7 3 2 Note Security Level 3 or higher is required to adjust the Level Offset 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A V keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP Until the display reads Liquid Level Menus 4 Press ENTER The display will read High Level Alarm 5 Press SETUP Until the display reads Advanced Level Menus 6 Press ENTER The
5. Last Event Query Input EVENT Returned XXXXX MM DD YY HH MM XXX X XXX X XXX X XXX X Comments String returned Date Time TempA Temp B LN2 Level Liquid Usage Event Codes Event Log Count Query Input EVNCT Returned XXXXX Comments Used to query the number of event log records Event Log Record Query Input EVNLOG N Returned XXXXX MM DD YY HH MM XXX X XXX X XXX X Comments Used to query Event Record n where record number String returned Date Time TempA Temp B LN2 Level Liquid Usage Event Codes Set Event Log Interval Input LOGPER XXX Returned No response Comments Used to set the event log interval between 1 and 240 minutes Default setting is 240 minutes Event Log Interval Query Input LOGPER Returned XXX Comments Used to query the current event log interval Set the Time Input hh mm ss Returned No response Comments Used to set the controller Time in the format hh mm ss hour minute second Time Query Input TIME Returned hh mm ss Comments Used to query the current time 132 of 133 326636H01 Rev A UNID Set Unit ID Input UNID XXXXX Returned No response Comments Used to set the controller 5 digit Unit ID CONTROLLER Unit ID should be between 1 and 200 UNID Unit ID Query Input UNID Returned XXXXX Comments Used to query the controller Unit ID 13 Decontaminating and Sanitizing CryoExtra Freezers are constructed from Stain
6. Set Level Offset Input CALVL Returned No response Comments Used to set the liquid level offset Level Offset Query Input CALVL Returned XXX X Comments Used to query the current level offset value Global Password Query Input CODE Returned XXXX Comments Used to query the current global password Temp A High Alarm Test Input HITSTA Returned No response Comments Used to initiate the High Temp A Alarm Test See the High Temperature Alarm Test section for more information Temp B High Alarm Test Input HITSTB Returned No response Comments Used to initiate the High Temp B Alarm Test See the High Temperature Alarm Test section for more information Restore All Defaults Input INITEE Returned No response Comments Used to restore all factory default settings Set LN2 Saturation Temperature Input LNSATP Returned No response Comments Used to set the LN2 saturation temperature for single point calibration reference point Temperature Commands BPTMP Bypass Temp Query Input BPTMP Returned XXX X Comments Used to query the current bypass temp sensor reading 125 of 133 HITAS HITAM HITAM HITB HITB HITBS HITBM HITBM 326636H01 Rev A Set Temp A High Alarm Input XXX X Returned No response Comments Used to set the Temp A High Alarm value Temp A High Alarm Query Input HITA Returned
7. i253 nus m s 2 x 11 mae mae sb z 08 25 to RJ 45 RH45 mmm mmu z g Adapter pe 8 28 B B Bg 7 92 of 133 326636H01 Rev 7 5 Remote Alarm Tests 7 5 1 Global Remote The Global Remote contacts can be checked for continuity using a digital multimeter or ohm meter e Normal State No Alarms There should be continuity between the COM and NC terminals The COM NO circuit should be open e Alarm State There should be continuity between the COM and NO terminals The COM NC circuit should be open 7 5 2 Discrete Contacts The Discrete Contacts can be tested for continuity using a digital multimeter with diode check Since the Discrete Contacts are open collectors the diode check function tH should be used e Normal State No Alarms All Discrete Contact terminals should be open with respect to COMMON e Alarm State There should be continuity between the specific active alarm terminal and COMMON Inactive alarms should remain open with respect to COMMON z E S tT x lt gt a 88886 o el al DISCRETE CONTACTS 93 of 133 8 Controller Menu Maps 8 1 Main Setup Menus Main Display Temp A 200 0 C Temp 200 0 Level 100in Usage 12 1 infday Setup Security Level 1 Press ENTER for Press ENTER far Liquid Level menus or press SETUP for Advanced settings
8. Verify LN2 connections Verify adequate supply resistance for debris 326636H01 Rev A Equipment Usage Page 8 Verify Adequate LN2 Supply Page 106 Solenoid Valve Replacement Page 109 Verify lid switch settings Lid Switch Settings Current LN2 level at or above the high level setpoint Auto Fill disabled Inadequate LN2 supply Verify current LN2 level and level control settings Press Start Fill to verify manual operation Confirm auto fill settings Verify adequate supply Liquid Level Settings Page 44 Auto Fill Control Page 45 Verify Adequate LN2 Supply Page 106 Clogged inline filter Clean replace inline filter Inline Filter Replacement Page 109 Fill solenoid valves not opening Verify fill solenoid resistance Solenoid Valve Replacement all the way Check fill solenoid valves for debris Page 109 Leak in plumbing or LN2 supply Check for leaks Plumbing Leak Check connection Page 107 Restore to defaults improperly Recalibrate temperature Temperature Sensor calibrated or requires Calibration bae sensor Incorrect recalibration Page 71 temperature Incorrect LN2 saturation Verify and adjust LN2 LN2 Saturation Temperature readings temperature for altitude saturation temperature Page 43 Faulty temperature probe Requires calibration Disconnected clear vinyl tube Confirm resistance values and replace if necessary Page 118 Perform LN2 level cal
9. warms to room Place Bypass Probe in ice water and Wait for Bypass Temp to stabilize 326636H01 Rev A 15 Press ENTER Bypass Probe two The display will read Bypass Probe two point calibration complete Point calibration 83 of 133 326636H01 Rev 7 4 Communication Networking The Controller is equipped with two independent RJ 45 serial ports These ports are intended for connection to another controller PC serial printer or other RS 485 device Up to 100 controllers can be successfully networked NOTE The Controller should never be connected directly to a LAN or public telecommunications network SERIAL SERIAL 1 2 Billie i 2 S B Uu RF 3S ie a 1 Se Yes ow oot Figure 12 Controller serial ports and RS 485 4 wire pin diagram Table 6 RS 485 Interface Specifications COM Setup bits sec Parity Data bits Stop bits 9600 N81 9600 None 8 1 9600 N82 9600 None 8 2 19200 N81 19200 None 8 1 19200 N82 19200 None 8 2 COM Type ASCII Printer OFAF MODBUS Disabled Transmission Mode Terminator 4 wire Asynchronous CR LF 84 of 133 326636H01 Rev 7 4 1 Controller Connect Computer Interface Controller Connect is a free downloadable software program that enables a user to download the event log and query and adjust controller parameters Features include a user friendly A
10. 0954 45 0525 345 280 5957 1009_ 559 355 554 0555 355 288 7159 1038 558 oso 350 292 725 1053 162 0585 365 206 7360 1 068 165 000 f 370 309 746 1082 Posie 375 304 7562 1097 385 312 7764 1125 Lim poen 3950 316 785 mia oes s 7s 1 155 _ 0587 405 324 8065 110 os 0717 410 332 8268 1193 07 415 335 836 1214 420 340 8469 1228 25 Cas saa 0775 30 ae 857 1258 5445 0790 435 353 8772 1272 275 23 555 080 440 357 8873 1257 93 2010 ose 200 27 556 445 351 8974 1202 25 97 2420 285 291 574 08 450 365 9074 1316 125 101 2521 0355 J 290 235 558 0848 455 369 915 1331 f os 2er 030 295 239 5999 469 373 926 1345 is ws vom _ 2 2728 305 ose 9478 1375 480 389 9579 220 22 5 EL 3 932 4 033 _ 4 134 4235 NUM EINEN 380 308 7663 EIN 16 0 130 3 226 0 468 f 325 263 6 554 0951 123 of 133 326636H01 Rev 12 3 Controller ASCII Interface American Standa
11. 7 Press SETUP The display will read Timed Fill Start Hour Use the keys to adjust and press ENTER to save 8 Press SETUP The display will read Timed Fill Start Minute Use the keys to adjust and press ENTER to save 56 of 133 326636H01 Rev A Press ENTER for Advanced Fill menus Timed Fill DISABLED Timed Fill Frequency 5 Days Timed Fill Start Hour 20 00 Timed Fill Start Minute 20 00 7 2 5 2 Maximum Fill Time 326636H01 Rev A The maximum fill time can be adjusted in the Advanced Fill Menus If a freezer begins filling but does not reach its High Level Setpoint within the maximum fill time then the Controller will terminate the fill and trigger a Fill Time Alarm Auto Fill Control will then be disabled until the Fill Time Alarm is cleared The maximum fill time has a range of 30 to 240 minutes NOTE Security Level 3 or higher is required to adjust the maximum fill time 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP Until the display reads Advanced Settings 4 Press ENTER The display will read Advanced Fill Menus 5 Press ENTE
12. Minimu Minimum Turn tray Direct Load m Door Ceiling Liftover Platform Weight Weight per Caster Width Height Height Height Empty Liquid Full Full Qty of Freezer Model in mm in mm in mm In mm Ibs kg Ibs kg Casters Ibs kg CryoExtra 20 CryoExtra 20MDD 32 813 90 1 2289 55 3 1405 6 152 515 234 1340 608 4 335 152 CryoExtra 40 CryoExtra 40MDD 42 1066 87 8 2230 39 2 995 9 228 720 327 2140 971 4 535 243 CryoExtra 80 CryoExtra B0MDD 60 1524 90 3 2294 38 8 985 9 5 242 1721 781 4830 2191 4 1208 548 CryoExtra94 CryoExtra 94MDD 60 1524 98 6 2540 43 8 1112 9 5 242 1721 781 4875 2211 4 1219 553 11 of 133 5 2 Plumbing Assembly 326636H01 RevA The plumbing assembly carries and regulates the flow of LN2 from the liquid supply through the fill line and into the freezer It is comprised of three main circuits fill circuit purge circuit and gas bypass circuit 2 Circuit Hot Gas Bypass Key PartNumber Component Spec Details 1 14224611S Fill Solenoid Valve 24 VDC R 70 single 35 dual 2 1810032 Pressure Relief Valve 50 PSI 3 45 bar 3 11648945 Inline Filter 40 micron 4 n a Fill Tee Transfer Hose Connections in ODT 45 flare 14 MPT 5 13284954S Purge 3 way Solenoid Valve 24 VDC R 140 6 10713400 Gas Bypass Temp Sensor Pt 1000 RTD 7 14224611S Gas Bypass Solenoid Va
13. XXX X Comments Used to query the current Temp A High Alarm value Temp A High Alarm Status Query Input HITAS Returned 0 or 1 Comments Used to query the Temp A High Alarm status where 0 Off and 1 On Set Temp A High Alarm Mask Input HITAM 0 or HITAM 1 Returned No response Comments Used to enable or disable the Temp A High Alarm where 0 disabled and 1 enabled Default setting is 1 enabled Temp A High Alarm Mask Query Input HITAM Returned 0 or 1 Comments Used to query the Temp A High Alarm Mask status where 0 disabled and 1 enabled Default setting is 1 enabled Set Temp B High Alarm Input zXXX X Returned No response Comments Used to set the Temp B High Alarm value Temp B High Alarm Query Input HITB Returned XXX X Comments Used to query the current Temp B High Alarm value Temp B High Alarm Status Query Input HITBS Returned 0 or 1 Comments Used to query the Temp High Alarm status where 0 Off and 1 On Set Temp B High Alarm Mask Input HITBM 0 or HITBM 1 Returned No response Comments Used to enable or disable the Temp B High Alarm where 0 disabled and 1 enabled Default setting is 1 enabled Temp B High Alarm Mask Query Input HITBM Returned 0 or 1 Comments Used to query the Temp B High Alarm Mask status where 0 disabled and 1 enabled Default setting is 1 enabled 126 of 133 LOTA LOTA LOTAS LOTAM LOTAM LO
14. keys to adjust the High Level Alarm value and press 8 0 in ENTER to save the change 5 Press SETUP High Level Set t The display will read High Level Setpoint Use the MENU AERE keys to adjust the High Level Setpoint value and 70in press ENTER to save the change 6 Press SETUP Low Level Set t The display will read Low Level Setpoint Use the keys to adjust the Low Level Setpoint value and 5 0 in press ENTER to save the change 7 Press SETUP Low Level Al The display will read Low Level Alarm Use the A keys to adjust the Low Level Alarm value and press 4 0in ENTER to save the change 44 of 133 326636H01 Rev 7 2 2 2 Enable Disable Auto Fill Control Auto Fill Control can be enabled or disabled in the Advanced Level Menus NOTE Security Level 3 or higher is required to enable disable Auto Fill 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER The display will read Temperature Menus Press ENTER for Temperature Menus 3 Press SETUP P ENTER f Until the display reads Liquid Level Menus a Liquid Level menus 4 Press ENTER
15. or press SETUP for 94 of 133 326636H01 Rev A 8 2 Temperature Setting Menus Press ENTER tor Temperature menus o press SETUP tor next menu Security Level 2 ENTER tor Temp Amenu o press SETUP br next menu Temp A ENABLED Use 1 1 Prem ENTER to sam iati e High Temp Abm Test NO Testing Temp A 200 0 lt Temp AHigh Alarm noo Use 1 Frwm ENTER to 43 Temp A Low Alarm 200 0 C Setup Use 11 Fress ENTER 16 sum p AHigh Alem ENABLED Use 1 1 to adit Press ENTER to awe Temp Aam ENABLED Use 1 to adit Prew ENTER 10 sea Press ENTER br Temp press SETUP fr nextmenu 55 pres ENTER foc Temp B menu o press SETUP br next menu Use so adiuet Prem ENTER to seve indateHeh Temp Alm Test NO Testing 200 0 C Temp B High Asm 110 0 Use to Prem ENTER to see Temp B Low Aem 200 0 c Use T 1 Prem ENTER to see Temp B High Alam ENABLED Use 1 to Fres ENTER to sma Use 1 1 Press ENTER to save Press ENTER tor Temp B Ca traton of press SETUP for next menu 95 of 133 326636H01 Rev A 78 2 Temperatum Use T 1 adjust ress ENTER to 195 8 8 3 Temperature Calibration Menus Press ENTER for Press ENTER for Temp B Calibration Calibration Type Temp A Calibration 5 sec SINGLE P
16. 0 or 1 Comments Used to query the Temp B Low Alarm Mask status where 0 disabled and 1 enabled Default setting is 1 enabled 127 of 133 TUNI TUNI 326636H01 Rev A Temp A Current Data Query Input TEMPA Returned XXX X Comments Used to query the current Temp A sensor reading Temp B Current Data Query Input TEMPB Returned XXX X Comments Used to query the current Temp B sensor reading Set Temp Units Input TUNI C TUNI F or TUNI K Returned No response Comments Used to set the Temp units where C Celsius F Fahrenheit and Kelvin Default setting is degrees Celsius Temp Units Query Input TUNI Returned C F or K Comments Used to query the Temp units where Celsius F Fahrenheit and Kelvin Default setting is degrees Celsius Level Commands BPFIL FILAS FILL FILL FILLM FILT Bypass Status Query Input BPFIL Returned 0 or 1 Comments Used to query the current hot gas bypass status where 0 not bypassing and 1 bypassing Fill Time Alarm Status Query Input FILAS Returned 0 or 1 Comments Used to query the Fill Time Alarm Status where 0 off and 1 on Set Fill Status Input FILL 0 or FILL 1 Returned No response Comments Used to set the current Fill Status where 0 Stop Fill and 1 Start Fill Fill Status Query Input FILL Returned 0 or 1 Comments Used to query the current Fill Stat
17. 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press ENTER The display will read Temp A Menu NOTE To access Temp B Menu press SETUP instead of ENTER 4 Press ENTER The display will read A Enabled Use the A V keys to change Temp A to DISABLED and press ENTER to save the setting change If a setting was changed a confirmation display will read New Setting Accepted 39 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Temp A Menu Temp ENABLED New Setting Accepted 7 2 1 2 High Temperature Alarm Test 326636H01 Rev A The high temperature alarm test allows a user to simulate a high temperature alarm without having to remove it from the freezer Once initiated the Controller will apply a small voltage to the sensor which causes the sensor to heat up The Controller will continue to heat the sensor until it exceeds the High Temp Alarm setting Once surpassed a High Temp Alarm will result and the sensor readings will begin to decrease The alarm buzzer will sound and the remote alarm contacts will switch to alarm state until the simul
18. 10 Press ENTER The display will read Wait for Bypass Temp to stabilize Wait for the displayed temp reading to stabilize while the probe is submerged in liquid 11 Press ENTER The display will read Bypass Probe single point calibration complete 81 of 133 326636H01 Rev A Higher User Level Required use A V to TAa A Press ENTER for Temperature Menus Press ENTER for Add on menus Battery Status On AC Power Press ENTER to Hot Gas Bypass menus Hot Gas Bypass ENABLED Press ENTER for Bypass Probe Calibration Type SINGLE POINT Bypass Calibration Place Bypass Probe Wait for Bypass Temp to stabilize Bypass Probe Single point 7 3 3 2 Hot Gas Sensor Two Point Calibration 1 Press SETUP Controller will prompt for a password A flashing cursor will make it clear which digit is being changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP Until the display reads Add on Menus 4 Press ENTER The display will read Battery Status 5 Press SETUP Until the display reads Hot Gas Bypass Menus 6 Press ENTER The display will read Hot Gas Bypass 7 Press SETUP Until the display reads Bypass Probe Calibration 8 Press ENTER The display
19. 18 82 RED GREEN 6 LID SWITCH STRIPES 22 96 7 4 PURGE VALVE YELLOW 20 48 8 PURGE VALVE RED 20 48 9 4 BYPASS SENSOR RED 22 67 10 4 BYPASS VALVE BLACK 18 75 11 BYPASS VALVE GRAY 18 75 12 BYPASS SENSOR WHITE 22 67 15 of 133 5 5 Specifications 326636H01 Controller Dimensions stand alone Length 9 1 in 232 mm Width 3 5 in 89 mm Height 8 0 in 203 mm Weight 6 5 lbs 2 95 kg Display Type Liquid Crystal Display LCD with Size backlight Resolution 20 x 4 Character Keypad 8x5 Pixels per Character 8 keys Multi function Electrical Controller Only Input Voltage 30 VDC Input Current max 5A Input Current continuous 1A Power Consumption max 28W Power Consumption continuous 6W Fill Valve Output Voltage 24 VDC Short Protection Current limiting automatic reset Electrical Jerome Power Supply WSL730M V1 Input Voltage Input Frequency 110 230 VAC automatically switching 50 60 Hz Output Voltage 30 VDC Max Current Capability 3A Input Current 0 73 A 110 VAC 0 35 A 230 Power Requirements Controller Power Supply Input Voltage 110 230VAC 50 60Hz Input Current max 73A 110VAC Input Current continuous 35A 9230VAC Input Power max 30 Watts Input Power continuous 8 Watts Controller Physical Connections Temperature Probes 2 pin twist lock Input Power 5 DIN Output Power Sensors B
20. 191 5 7 03 13 10 200 23 2008 5 00 AM 2 3 08 500 195 9 192 8 68 03 14 11 200 2 3 2008 419 AM 2 3 08 4 19 1959 1909 7 0 3 Figure 15 Properly formatted event log for plotting 87 of 133 ASCII Command Interface American Standard Code for Information Interchange ASCII commands can be used to query and adjust all Controller settings and parameters Commands must be entered in all capital letters just as they appear in the below tables For a complete list of proper syntax and responses refer to the Appendix IDN ALMS ALMS CALTA CALTB CALVL CALVL BPTMP HITA HITA HITAS HITAM HITAM HITB HITB HITBS HITBM HITBM LOTA LOTA BPFIL FILAS FILL FILL FILLM FILT FILT FILTIM HFIL HFIL HILA HILA HILS DSPN DSPN PCNT CLEVLG DATE DATE EVENT EVNCT EVNLOG n CONTROL COMMANDS ID Query CODE Set Alarm Status HITSTA Alarm Status Query HITSTB 326636H01 Rev A Global Password Query Initiate Temp A High Alarm Test Initiate Temp B High Alarm Test Calibrate Temp A in LN2 INITEE Restore All Defaults Calibrate Temp B in LN2 LNSATP Set LN2 Saturation Temp Set Level Offset LNSATP LN2 Saturation Temp Query Level Offset Query TEMPERATURE COMMANDS Bypass Temp Query LOTAS Temp A Low Alarm Status Query Set Temp A High Alarm LOTAM Set Temp A Low Alarm Mask Temp A High Alarm Query LOTAM Temp A Low Alarm Mask Query Temp A High Alarm Status Query LOTB Set Temp B Low Alarm Set Temp A High A
21. Backup Optional 37 7 2 Adjusting Settings and OPTIONS sc exea dba een on ra vae nnn eene 38 21 Temperdiure deli 39 722 Liquid Level e e e eerta 44 7 2 3 Additional Feature Sets o oa oe heb mel Ua eben dla RR RU be Tune 47 7 2 4 Display and Output Settings e to dent uds hee eate oo ee tute e Ape e RR 50 7 2 5 Advan ed SCUIAGS 55 7 2 6 Password Security Xe todo tei 68 7 3 Calibration PIOGBOUIBS uidet 71 7 3 1 Temperature Sensor CAlIDIatlOE iere erben 71 7 3 2 Liquid Nitrogen Level Galibration co retire ter tat abd e RR ds 75 7 8 8 Hot Gas Bypass Sensor Calibration esee 79 7 4 Communication tete ped ruere 84 7 4 1 Controller Connect Computer Interface esee 85 PAD OFAF Network he Dx n ete 89 TAB ES pipere TE aa 92 4 of 133 326636H01 Rev 7 5 Remote Alarm 93 75 Global Remote 93 75 2 Discrete Gorntacls oed rt tr epa ode eee eee 93 8 Controller Menu uu acre pe 94 8 1 Main Setup TSO stia pa alee uoce 94 82 Temperature Setting 2 95 83 Temperature Calibration Menus eese eene netten enn
22. LN2 or handling hoses valves and plumbing components Recommended protective clothing e Cryogenic gloves loose fitting Full face shield or chemical splash goggles Cryogenic apron Long sleeve shirt and cuff less pants Closed toe shoes no sandals Equipment Usage Cryogenic containers must be operated in accordance with the manufacturer supplier instructions Safety instructions will also be posted on the side of each Dewar Cryogenic containers must be kept in a well ventilated area protected from weather and away from heat sources In applications that use a modular liquid cylinder as a source of LN2 the supply will need to be replenished at regular intervals to ensure proper operation of the freezer When exchanging liquid cylinders follow the below procedure 1 Allow all plumbing components to warm to room temperature before attempting to change supplies 2 Close all valves associated with the liquid supply cylinder 3 Relieve pressure in the plumbing assembly by initiating a brief fill by either pressing Start Fill or using the manual fill button 4 Loosen the plumbing connection for the transfer hose at the liquid cylinder 5 Remove empty liquid cylinder and replace with full liquid cylinder pressurized to 22 35 psig 1 52 2 41 bar Attach the transfer hose to the plumbing connection on the liquid cylinder Ensure that the hose is connected to the connection labeled LIQUID Tighten the transfer hose plumbing connec
23. Level Verification The differential pressure measurement system used on CryoExtra freezers is nearly maintenance free It provides a high level of accuracy and resolution to give the operator a precise indication of the exact amount of LN2 present in the freezer at all times Despite its reliability it is important that the accuracy of the level measurement system is verified on a weekly basis This will prevent a control system malfunction from adversely affecting the temperature in the freezer storage space Use the meter dipstick provided with every CryoExtra freezer to manually measure the amount of LN2 in the freezer Follow the Dip Stick Procedure listed in section 7 3 2 to properly measure the level If the level is off by 1 0 inch 25mm or more follow the calibration procedure listed in the aforementioned section Verify Adequate Supply Adequate LN2 supply pressure and flow is imperative to the proper operation of CryoExtra freezers Any LN2 supply whether from bulk tank or liquid cylinder must be able to maintain a pressure of 22 35 psi 1 52 2 41 bar during a filling cycle and must have enough liquid to ensure the completion of a fill cycle The majority of nuisance alarms reported from CryoExtra freezers are due to inadequate supply 1 Observe the pressure of the supply source Ideally pressure should be 22 35 psi 1 52 2 41 bars NOTE It is very common for the pressure gauge on an industrial liquid cy
24. Returned E M or Comments Used to query the current LN2 level units English inches Metric millimeters and 96 Percent Current Liquid Usage Query Input RATE Returned XXX X Comments Used to query the current LN2 liquid usage rate Level Percent Commands DSPN DSPN PCNT DZER DZER Define 10096 Point Input DSPN XXX X Returned No response Comments Used to set the 100 level setting in inches or millimeters 100 Point Query Input DSPN Returned XXX X Comments Used to query the 100 level setting Current Percent Query Input PCNT Returned XXX X Comments Used to query the current percent level reading Define 096 Point Input DZER XXX X Returned No response Comments Used to set the 0 level setting in inches or millimeters 096 Point Query Input DZER Returned XXX X Comments Used to query the 0 level setting 131 of 133 326636H01 Rev Event Log Commands CLEVLG DATE DATE EVENT EVNCT EVNLOG LOGPER LOGPER TIME TIME Clear the Event Log Input CLEVLG Returned No response Comments Used to clear all data from the event log Data will be permanently lost when the event log is cleared Set the Date Input mm dd yy Returned No response Comments Used to set the current date in the format mm dd yy month day year Date Query Input DATE Returned mm dd yy Comments Used to query the current date
25. The Controller has a built in data logging feature that automatically stores vital time stamped information including temperatures LN2 level liquid usage and any alarms Data is logged at a user defined interval and anytime an event or alarm status changes The default log interval is 4 hours The Controller is able to store up to 30 000 events in its non volatile memory With a 4 hour log interval the Controller is able to store approximately 10 years worth of data This event log can be easily downloaded from the controller using ControllerConnect This downloaded file is a csv file that can be opened analyzed and plotted in EXCEL Besides being a record of the freezer status the event log is a vital tool for diagnosing problems or detecting potential problems with a freezer For instructions on how to download the event log refer to the Communication Networking section of this manual or the ControllerConnect User Manual PN 13946348 NOTE Since the event log is stored in non volatile memory it will be retained and unaffected when the controller loses power is restarted if the firmware is updated or when the event log is downloaded However resetting the controller to defaults will clear the event log If the event log memory is exceeded the oldest event will be deleted to make space for the most recent event Below is a sample event log and a list of event codes The event log header displays the version of ControllerConnect used to
26. Time Delay Use the A V keys to adjust the value and press ENTER to save 10 Press SETUP The display will read Stuck Valve Alarms Use the keys to adjust the value and press ENTER to save 11 Press SETUP The display will read Stuck Open Delay Use the keys to adjust the value 1min to 90min and press ENTER to save 12 Press SETUP The display will read Stuck Closed Delay Use the keys to adjust the value 1min to 90min and press ENTER to save 48 of 133 326636H01 Rev A Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Add on menus Battery Status On AC Power Press ENTER for Hot Gas Bypass menus Hot Gas Bypass DISABLED Bypass Temperature 20 0 Bypass Temp Setpoint 70 0 Bypass Alarm Time Delay 5 min Stuck Valve Alarms DISABLED Stuck Open Delay 1 min Stuck Closed Delay 1 min 7 2 3 3 Lid Switch Settings 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP The display will read Add on Menus 4 Press ENTER The current batter
27. adjust and press ENTER to save ENABLED Alarm Buzzer 52 of 133 326636H01 Rev 7 2 4 4 Languages The Controller has five language options to choose from English Spanish German Italian and French NOTE Security Level 2 or higher is required to change the language setting 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER P ENTER f The display will read Temperature Menus pi Si Temperature Menus 3 Press SETUP Press ENTER for Until the display reads Display and Output Display and Output 4 Press ENTER Temperature Units The display will read Temperature Units 5 Press SETUP P ENTER f Until the display reads Advanced Display and Output ee ne Advanced Display 6 Press ENTER The display will read Alarm Buzzer Alarm Buzzer ENABLED 7 Press SETUP The display will read Language Use the keys to adjust and press ENTER to save ENGLISH Language 53 of 133 326636H01 Rev 7 2 4 5 Printer This section describes how to adjust the Printer settings For instructions on how to connect a printer see the Communication Networki
28. and acquire these features Contact your CryoExtra Distributor or CryoExtra Technical Service for instructions NOTE The standard UL Lid Switch configuration is not controlled by the Controller Lid Switch Menus and the Lid Switch Installed option should be set to Optional Fog Clear Lid Switch configuration The optional fog clear lid switch configuration is connected directly to the Controller wire harness lid switch connector and is not wired in series with the fill valves This lid switch configuration is controlled by the settings in the lid switch menu of the Controller which include the fog clear feature and the Lid Open Alarm With the fog clear feature enabled the freezer will fill for 30 seconds each time the lid is opened which clears the fog and improves visibility The Lid Open Alarm feature allows the user to set a maximum time that the lid can remain open before an alarm is triggered For instructions on adjusting the lid switch settings refer to the Optional Features section NOTE Converting from the standard UL Lid Switch configuration to the optional Fog Clear Lid Switch configuration voids the UL compliance and requires that the UL mark be removed from the freezer NOTE Both lid switch configurations do have an override feature Simply pull up on the lid switch until it clicks to engage the manual override 35 of 133 326636H01 Rev 7 1 11 Hot Gas Bypass The Hot Gas Bypass is a feature on al
29. display reads Advanced Settings 4 Press ENTER The display will read Advanced Fill Menus 5 Press SETUP Until the display reads OFAF Menus 6 Press ENTER The display will read OFAF ID Use the A keys to adjust the OFAF ID and press ENTER to save 7 Press SETUP The display will read OFAF Units Use the keys to adjust the number of units and press ENTER to save 8 Press SETUP The display will read One Fill All Fill Use the A V keys to adjust the type and press ENTER to save 62 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Advanced Settings Press ENTER for Advanced Fill menus Press ENTER for OFAF menus OFAF ID llona A W ta adinct OFAF Units 3 One Fill All Fill SEQUENTIAL 326636H01 Rev A 7 2 5 5 Restore Default Settings Global Password The Controller can be reset to the factory defaults in the Advanced Settings Menu This will also reset post factory calibrations and global password to 3 4 5 6 The accuracy of the level and temperature measurements should be confirmed after resetting Note It is recommended to download complete data from the controller prior to performing a restore to defaults Restoring to defaults will erase previous stored data and it cannot be retrieved once the restore function is performed NOTE Security Level 3 o
30. display will read Auto Fill Control 7 Press SETUP The display will read Level Offset Use the A Y keys to adjust value and press ENTER to save the change 46 of 133 a freezer see the First Fill Distributor or Technical Service Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Liquid Level menus High Level Alarm 8 0 in Press ENTER for Advanced Level menus Auto Fill Control ENABLED Level Offset 1 3 inches 7 2 3 Additional Feature Settings 326636H01 Rev A This section details how to view and adjust the settings for the Battery Backup Power Failure Alarm Hot Gas Bypass and Lid Switch NOTE Security Level 2 or higher is required to adjust the Hot Gas Bypass and Lid Switch settings 7 2 3 1 7 2 3 2 Battery Backup Status 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP The display will read Add on Menus 4 Press ENTER The current battery status will be displayed The display will read On AC Power if battery is connected or not connected and controller running on AC OR The display will read On Battery Backup
31. fittings for any leaks ev Complete Function Test ThermoFisher Scientific recommends that freezers undergo a complete function test every 12 months to ensure correct functionality and identify potential problems before symptoms develop Function test documents can be written based on this manual or this manual itself can be used to verify the function of CryoExtra freezers Solenoid Valve Replacement All CryoExtra freezers are equipped with electromechanical solenoid valves that have been tested and approved by CryoExtra for cryogenic use These valves utilize a PTFE seal for optimal sealing in cryogenic environments Over time the normal thermal cycling that this seal is subject to will cause it to harden and lose its ability to seal completely This will result in seepage past the sealing surface which can increase the LN2 consumption of the system and in extreme cases result in an overfill situation Thermal cycling through normal operation can also cause moisture ingress into the coil of the solenoid valve Over time this may cause the connections and wiring in the coil to corrode and eventually fail This will result in an inoperative solenoid valve NOTE Always use replacement solenoid valves from ThermoFisher Scientific Substituting non CryoExtra components may result in inoperable valves and even damage to the Controller system Damage to the control system due to use of non CryoExtra parts will not be covered by war
32. for radio cellular cordless telephones and land mobile radios amateur radio AM and FM radio broadcast and TV broadcast cannot be predicted theoretically with accuracy To assess the electromagnetic environment due to fixed RF transmitters an electromagnetic site survey should be considered If the measured field strength in the location in which the CONTROLLER is used exceeds the applicable RF compliance level above the CONTROLLER should be observed to verify normal operation If abnormal performance is observed additional measures may be necessary such as reorienting or relocating the CONTROLLER Over the frequency range 150 kHz to 80 MHz field strengths should be less than V m 119 of 133 326636H01 Rev A Table 6 Recommended separation distances between portable and mobile RF communications equipment and the Controller for Controller systems that are not life supporting See 5 2 2 2 Recommended separation distances between portable and mobile RF communications equipment and the CONTROLLER The CONTROLLER is intended for use in the electromagnetic environment in which radiated RF disturbances are controlled The customer or the user of the CONTROLLER can help prevent electromagnetic interference by maintaining a minimum distance between portable and mobile RF communications equipment transmitters and the CONTROLLER as recommended below according to the maximum output power of the communications equipment
33. increases This differential pressure system allows the controller to measure the exact LN2 level accurately L9 Lo Lo papaa jaaha po png uri uen ha ju pur reden rpm e rea dua Figure 4 As the fluid level in the cylinder increases the imposed head pressure at the bottom of the cylinder increases proportionally The pressure gauges measure this increase in level This pressure signal generated by the LN2 is transmitted from the bottom of the inner vessel through the freezer s annular line to the differential pressure sensor inside the Controller The Controller then compares this pressure signal to its atmospheric pressure readings and is then able to determine the exact LN2 level The term differential pressure refers to the fact that the level is determined from the difference between the hydrostatic head pressure of the fluid column and atmospheric pressure The measured LN level is displayed in inches millimeters or as percentage full Unlike alternative level sensing systems such as thermistor based systems differential pressure allows the exact level to be measured and displayed not just a level range This completely enclosed system requires minimal maintenance and is not affected by humidity moisture or other environmental variables Also the differential pressure system allows the automatic level settings and alarms to be set and adjusted electronically instead of having to p
34. of 133 326636H01 Rev A 7 2 5 Advanced Settings This section describes how to change the various Advanced Settings 7 2 5 1 Timed Filling The Controller has several advanced filling options including Timed Filling Timed Filling allows the user to set a regular filling schedule based on a fill start time and a fill frequency If a facility staff would like the peace of mind of knowing that all the freezers are full of LN2 before leaving for the weekend then they could set up a timed fill for every Friday afternoon Even if the LN2 level in a freezer is above Low Level Setpoint the Controller will initiate a fill and top off the freezer to its High Level Setpoint If the liquid level is at or above the High Level Setpoint then the Controller will skip that timed fill event and not initiate a fill A Controller with a Timed Filling schedule will still maintain Auto Fill Control If Timed Filling and Auto Fill Control are enabled then the Controller will still initiate a fill anytime the liquid level reaches the Low Level Setpoint Firmware Ver 2 03 or higher will allow the Timed Fill Start to be set to any hour and minutes except midnight Timed Filling Setup Enable Timed Filling and then select the number of days to elapse between timed fillings Select a Timed Fill Start time that is later that same day If Timed Filling is being enabled at 9 30 AM then the Timed Fill Start should be set to 9 31 AM or later This is recommended for
35. operation and lead to a dangerous over pressurized condition Additionally this will void any warranty Gasket Removal or Replacement 1 Depending on the condition of the current gasket the gasket material can be removed and replaced or more material can simply be added to the existing gasket 2 The replacement gasket material will be a neoprene tape 3 Simply clean the surfaces remove the tape back to expose the adhesive and install gasket material 4 Trim to size as needed 5 Cut a 4 inch gap in the gasket material on either side of the lid as shown below to allow sufficient venting of the freezer space 111 of 133 326636H01 Rev Additional p T T TT 4 inches Lid Gasket Standard Ae F 4 nt 4 inches Complete Freezer Thaw and Moisture Removal Remove freezer LN2 supply Unplug Controller main power and battery backup if equipped Open or remove lid from freezer Allow LN2 to completely evaporate and the freezer space to warm to room temperature Placing a fan blowing into the freezer will accelerate this process After it has reached ambient temperature thoroughly remove any moisture from the freezer space This can be done with a wet dry vacuum and towels Open the hinged hatch on the bottom of the turn tray to access the bottom of the freezer 6 Once moisture has been removed from the freezer space purge the plumbing assembly and annular lines with nitrogen gas Compressed n
36. switch to alarm state For more information on the global remote and discrete contacts refer to the Remote Alarm Monitoring section To clear an alarm first correct the condition and then press the Alarm Mute key or restart the controller If the alarm condition is still present then pressing the Alarm Mute key will only mute the audible buzzer for 30 minutes The condition must be corrected in order to clear the alarm NOTE Most Controller alarms have a one minute delay to avoid false positive or nuisance alarms denotes alarms that do not have a one minute time delay Table 1 Controller alarms and descriptions Alarm Display Description High Temp A Temperature A is reading at or above the Temp A High Alarm setting Can be Enabled When disabled there will be no visible or audible alarm indication The alarm event or Disabled code will continue to be recorded in the internal data log High Temp B Temperature B is reading at or above the Temp B High Alarm setting Can be Enabled When disabled there will be no visible or audible alarm indication The alarm event or Disabled code will continue to be recorded in the internal data log Low Temp A Temperature A is reading at or below the Temp A Low Alarm setting Can be Enabled When disabled there will be no visible or audible alarm indication The alarm event or Disabled code will continue to be recorded in the internal data log L
37. termination of the fill cycle 5 Coil retaining clip Coil assembly Plunger housing Plunger assembly and PTFE seal Hex nut Brass valve body 110 of 133 326636H01 Rev A NOTE If the brass valve body requires replacing the freezer plumbing will need to be disassembled and the entire valve replaced PN 142246115 It is typically easier to start disassembling the plumbing assembly beginning at the fill tee for fill valve replacement or the gas bypass muffler for gas bypass valve replacement NOTE When installing a complete new valve ensure it is oriented correctly An is engraved on the side of the SMC brass valve body The valve should be installed so that this is on the inlet side of the valve Relief Valve Replacement CAUTION Ensure that the LN2 supply valve is closed and the plumbing assembly is vented before removing the relief valve 1 Remove plumbing shroud to gain access to plumbing system 2 If equipped with a relief valve deflector loosen the deflector clamp and slide off the deflector 3 Loosen the relief valve and remove it from the plumbing assembly Be sure to support the attachment tube with wrench to prevent damage from twisting 4 Install new relief valve PN 1810032 applying new Teflon tape if needed Ensure relief valve is rated to 50 PSI 3 4 bar CAUTION Installing a relief valve with a different pressure rating could prevent proper
38. these probes changes linearly with temperature Platinum s very linear relationship between resistance and temperature makes Pt 1000 RTDs ideal for temperature measurement in cryogenic environments The temperatures displayed on the Controller LCD are not real time readings but rather a running average of the previous few measurements The Controller takes temperature measurements several times a second and then averages the previous few measurements while updating the displayed temperature every second The hot gas bypass temperature sensor is also a Pt 1000 RTD Pt 1000 RTD Temperature Measurement 1200 1000 0 9999 800 400 Resistance Output ohms 200 0 E T T T T T T T 220 200 180 160 140 120 100 80 60 40 20 0 20 40 Temperature C Figure 6 Platinum s linear relationship between temperature and resistance 27 of 133 326636H01 Rev 7 1 5 User Defined Alarms The Controller employs 18 different audible visual alarms These alarms are designed to alert the user to possible problems with the freezer temperature LN2 level controller power and LN2 supply In the event that a condition triggers an alarm an audible buzzer will sound that particular alarm will appear on the Controller LCD display and the global remote alarm contacts will switch to alarm state If the triggered alarm is one of the four specified discrete contacts then that contact will also
39. will read Wait while probe warms to room temperature Wait while the temperature sensor warms to room temperature and the controller counts down 11 Wait 180 seconds After the controller counts down the display will read Place Probe A in ice water Completely submerge the sensing end of the probe in the ice water bath 12 Press ENTER The display will read Wait for Temp A to stabilize Wait for the displayed Temp A reading to stabilize while the probe is submerged in the ice bath 13 Press ENTER The display will read Probe A two point calibration complete 74 of 133 326636H01 Rev A Wait while probe warms to room Place Probe A in ice water and press Wait for Temp A to stabilize then press Probe A two point calibration complete 326636H01 Rev 7 3 2 Liquid Nitrogen Level Calibration This section describes the procedure to calibrate the LN2 level This procedure requires the cryogenic meter dip stick supplied with each CryoExtra freezer This calibration method provides level measurements with a 0 5 13 mm accuracy All new freezers equipped with controllers have been calibrated at the factory The liquid level should only be calibrated if faulty readings are suspected the Controller itself has been replaced following a firmware update or as a part of a preventative maintenance schedule CAUTION Always wear protective gloves and face shield when handlin
40. 26VDC or 80 or 100 if controller is running on battery backup AC disconnected 5 Press SETUP The Power Failure Alarm status will display Use the keys to ENABLE or DISABLE and press ENTER to save the change Hot Gas Bypass Settings 47 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Add on menus Battery Status On AC Power Battery Status On Battery Backup AMIRAN ana A Anno Power Failure Alarm ENABLED 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP The display will read Add on Menus 4 Press ENTER The current battery status will be displayed 5 Press SETUP Until the display reads Hot Gas Bypass Menus 6 Press ENTER The display will read Hot Gas Bypass Use the A keys to adjust and press ENTER to save 7 Press SETUP The current Bypass Temperature Sensor reading will be displayed 8 Press SETUP The display will read Bypass Temp Setpoint Use the keys to adjust the value and press ENTER to save 9 Press SETUP The display will read Bypass Alarm
41. 6 428 862 478 905 861 901 923 945 169 941 244 984 358 960 444 1019 53 1054 60 105849 1093 47 1097 35 113221 1136 08 1170 85 1174 70 120936 1213 21 124777 125160 1286 05 128987 132422 1328 03 136228 1366 08 121 of 133 578 205 618 756 659 120 699 310 739 337 779 210 818 937 1023 43 1062 38 1101 23 1139 95 1178 56 1217 05 1255 43 1293 70 1331 84 1369 87 198 154 241 103 283 713 326 006 368 002 409 719 451 175 492 386 533 370 574 139 614 709 655 092 695 299 735 341 326636H01 Rev 189 522 232 541 275 217 317 572 359 626 401 397 442 904 484 163 525 191 566 002 606 611 647 030 687 271 727 346 767 263 807 033 846 663 886 160 925 530 964 778 1074 05 1112 86 1151 55 1190 12 1228 58 1266 92 1305 15 1381 26 326636H01 Rev 12 2 Table 10 LN2 Volume per Inch of Liquid in CryoExtra Freezers NOTE The below values are accurate for the cylindrical portion of the freezer and do not take into account inventory components that will displace LN2 CE 8120 CE8120M 10 1 CE 8140 CE8140M 19 3 CE 8180 CE8180M 1 38 9 CE 8194 CE8194M 38 9 122 of 133 326636H01 Rev Table 11 Conversion of inches of LN2 to various pressure units NOTE Conversion values do not take into account the Controller Level Offset SSN eee 11 1 REL 4 rea TIE ELLE oa 7o 138 3428 0497 335 271 6755 0 980 75 142 359 0512 340 276 6856
42. CALL A PHYSICIAN IMMEDIATELY 8 of 133 326636H01 Rev 4 Certifications and Listings All fully automatic CryoExtra cryogenic freezer systems equipped with a controller are UL CUL listed and CE marked to the Low Voltage Directive LVD Specially designated freezer models are also CE marked to the Medical Device Directive MDD The LVD is a European Union directive regulating the construction and operation of electrical equipment that is not considered a medical device The MDD is a European Union directive regulating medical device construction and operation These listings and certifications encompass the entire freezer system and not just the electronic controller EU Representative Dr Rainer Kalamajka Manager Regulatory Compliance amp Document Control Thermo Fisher Scientific Laboratory Equipment Division Robert Bosch Str 1 D 63505 Langenselbold Germany CryoExtra brand manufactured liquid nitrogen freezers covered in this manual are non hazardous open mouth vacuum insulated dewars They are constructed of stainless steel and aluminum and specifically designed to hold liquid nitrogen They are not subject to any pressure vessel codes as they are open to atmospheric pressure CryoExtra liquid nitrogen containers are shipped empty without liquid nitrogen or any hazardous material from our factory An MSDS is not available for the final formed and welded assembly An MSDS on the stainless steel or aluminum a
43. CRYOEXTRACE8100 Series Liquid Nitrogen Storage Systems Operating and Maintenance Manual 326636H01 Rev A 326636H01 Rev CryoExtra TECHNICAL FREEZER MANUAL P N 326636H01 Rev A April 2014 1 Preface Thermo Fisher Scientific Inc 275 Aiken Road Thermo Asheville NC 28804 United States www thermofisher com Technical Support Contact Information IF YOU NEED ASSISTANCE US and Canada Phone 1 866 984 3766 866 9 THERMO LABORATORY PARTS and SERVICE Phone 1 800 438 4851 TECHNICAL SUPPORT Phone 1 800 438 4851 Thermo Scientific products are backed by a global technical support team ready to support your applications Visit www thermoscientific com or call North America 800 438 4851 India 91 22 6716 2200 China 8621 68654588 Japan 81 3 3816 3355 Australia 1 300 735 292 Austria 43 0 810 282 206 Belgium 32 0 53 73 42 48 France 33 0 8 25 800 119 Germany 49 0800 1 112 110 Italy 39 0295059537 Netherlands 31 00800 98765555 Nordic Baltic 358 9 329 100 Russia CIS 7 812 703 42 15 Spain Portugal 34 914 845 965 Switzerland 41 56 618 41 00 UK Ireland 44 870 2411034 Denmark 45 70 23 62 67 2 of 133 326636H01 Rev This manual covers the use and maintenance of CryoExtra Cryogenic Freezers and the associated control system READ BEFORE USING THIS EQUIPMENT It is intended for use by trained personnel only All service and maintenance should be performed by a
44. Control Alarm Settings X X X Date Time X X X Language Settings X X X Printer Settings X X X Temp Calibration X X X Level Calibration X X Enable Disable Auto Fill X X Timed Fill Settings X X OFAF Settings X X Communication Settings X X Reset to default settings X X Password Settings X Forgot your password Contact your authorized CryoExtra Distributor or Technical Service 31 of 133 326636H01 Rev 7 1 8 Communication Networking Capabilities The Controller RS 485 Serial interface offers several advanced communication capabilities The Controller is equipped with two independent RJ 45 serial ports These ports are intended for connection to another CryoExtra controller PC serial printer or other RS 485 device Up to 100 Controllers can be successfully networked x NOTE The Controller should never be connected directly to a LAN or public telecommunications network Below is summary of the different communication networking capabilities e Host Computer The Controller can communicate with a computer via an RS 485 converter and ControllerConnect or Windows HyperTerminal Through the use of simple ASCII commands all Controller settings and functions can be monitored and adjusted with these software programs In addition to ASCII communication ControllerConnect also offers a user friendly event log downloader The event log is downloaded as a comma separated variable csv fil
45. Controller should not be used adjacent or stacked with other equipment and that if adjacent or stacked use is necessary the Controller should be observed to verify normal operation in the configuration in which it will be used DISPOSAL OF PRODUCT Stainless Steel Freezer Freezers used to store biological materials require decontamination prior to disposal Contact Thermo Scientific for decontamination information or refer Section 13 of the manual Controller Local or national environmental laws and regulations may prohibit disposal of electrical and or electronic equipment such as the controller Contact the local city or town offices for instructions on proper disposal of electrical or electronic equipment Alternately Thermo Scientific may be contacted for disposal information 120 of 133 12 Appendix 12 1 Reference Tables Table 9 Temperature vs Resistance Output ohms for Pt 1000 Temperature Sensors c 0 i 2 3 4 5 6 7 8 1039 03 1116 73 1155 41 1193 97 1232 42 1270 75 1308 97 1347 07 223 965 266 708 309 126 351 238 393 064 434 622 475 930 517 003 557 856 1011 72 1050 71 1089 59 1128 35 1166 99 1205 52 1243 93 1282 23 1320 41 1358 48 211 076 206 772 253 920 249 651 296 434 292 197 338 635 334429 380 545 376 367 422 182 418 030 463 563 459 436 504705 500601 545 622 541 540 586 331 582 269 626 843 622 800 667 172 663 147 320 747324 743 331 787 166 783 189 826 865 822 902 86
46. Display Bypass M0 0 C Bpass Time 0000 Altemate Display Text Temp Open amp Lave 100 in Usage 121inday 326636H01 Confirmation Display NEW SETTING ACCEPTED Alarm Display flashing from the main display Low Temp A Low Temp Ended at Started at 11 2505 15 30 11 25 05 1530 ALARM ALARM Low Laval Low Level En ded at Started at 11 2505 15 30 11 2546 1530 ALARM Fill Time 20 min Ended at 11 25 05 15 30 11 25 05 1530 AL ARM Lid Open 30 mn Rated ar 11 255 1530 ALARM Temp A Calibration Temp ACgitretion Ended at 512100 at 11 25 05 15 30 11 255 1530 ALARM ALARM Bypass Calbratcn Bypass Calo ration En ded at Stated a 1112505 15 30 1172545 1530 11 256 15 30 ALARM Vane Stuck Closed Stuk Closed Endod at Sawi at 11 2505 15 30 11 25 08 15 30 Low 8 Endedat 11 25 05 15 30 ALARM High Level Ended at 11 25 05 15 20 ALARM Bypses Time 5 min Ended at 1142505 15 30 Comm unrcarions Loss Urit 2 Ended at 11 25 05 15 30 ALARM Tanp Calibration Ended at 11125505 15 30 11 2505 15 30 ALARM valve Stuck Open Ended at 12586 153 ALARM High Temp Started 1112555 15 0 ALARM Low Temp Started at 11 2505 15 30 11 2505 1530 Communications Loss Unit 2 Staited at 11125595 1530 ALARM 11 2505 1530 ALARM Valve Stuck Open Storied at 11 2505 15 30 104 of 133 High
47. EL 2 Before plotting be sure to delete any rows containing text such as Parameter number refer to figure 15 for clean data 3 Insert new column between the Time and Temp A columns 4 Label this new column heading Date Time 5 In cell E4 enter the formula C4 D4 6 Fill down the rest of column E with this formula 7 Format the cells in column E so that the Number Category is Date and the Type is a date time combination 8 Sort all the data by Descending Date then by Descending Time and then by Ascending Record 9 Select the desired data to plot Date Time through LN2 Usage columns and insert a scatter chart 10 Once the chart has been created select a secondary axis for the LN2 Level and LN2 Usage data sets 11 Format the chart and adjust the axis scales so that the data is easy to view and analyze C A B C D E F G H J 1 2 00200 3 Unit ID Date Time Date Time TempA Temp LN2 Level LN2 UsageEvent Codes 4 1 200 2 4 2008 9 00AM 2 4 08 9 00 1959 190 6 5 03 5 2 200 2 4 2008 500 AM 2 44 08 5 00 1959 190 65 0 3 6 3 200 2 4 2008 100 AM 2 4 08 100 1959 190 66 03 7 4 200 2 4 2008 1200 AM 214108 000 1959 190 3 66 03 8 5 200 2 4 2008 1200 AM 214108 0 00 1959 190 3 66 0320 9 6 200 2 3 2008 900 2 3 0082100 1959 190 3 66 03 10 7 200 2 3 2000 500 2 3 08 17 00 1959 190 6 7 03 11 8 200 23 2008 100 2 3 08 13 00 1959 190 9 67 03 12 9 200 2 3 2008 9 00 AM 23 08 900 195 9
48. Fuse 4A 250V 13319459S CONTROLLER Stand alone with text display 13319467S CONTROLLER Stand alone with symbolic display 13223908 CONTROLLER Cabinet Back Panel 13223836 CONTROLLER Cabinet Text Display Panel 13223861 CONTROLLER Cabinet Symbolic Display Panel Accessories Part Number Description 20561020 LN2 Level Dip Stick 47 13376947 Controller COM USB Kit PC interface kit 10856321 Daisy Chain Kit One network cable and splitter 10740053 RJ 45 Network Cable 10856312 RJ 45 Jack Splitter 11358251 OFAF Master Cable 10784443 Fill Valve Tee Assembly Tee two freezer to one LN2 supply connection 1611592 Relief Valve Pipe Away Adapter 3 8 inch NPT Outlet 1810092 Relief Valve Pipe Away Adapter 12 inch NPT Outlet 9713159 LN2 Transfer Hose 4 ft 1220 mm inch 12 7 mm ODT 9713109 LN2 Transfer Hose 6 ft 1829 mm inch 12 7 mm ODT 1110862 LN2 Transfer Hose Coupler Daisy chain two transfer hoses 11544943 Printer Kit Includes serial printer and cables 13934911 Automatic LN2 Supply Switch 114 of 133 10 Troubleshooting Quick Reference Possible Causes Fixes Instructions Freezer not filling Slow fills or Long fill times Incorrect LN2 level readings Consistent power failure alarms Improperly connected LN2 supply Inadequate LN2 supply volume or pressure Fill solenoid valves not opening
49. Interval Event Log Interval Query Set Time Time Query Set Unit ID Unit ID Query 326636H01 Rev 7 4 2 OFAF Network Setup OFAF networking allows multiple controllers to be linked together such that all of the freezers will fill whenever any networked controller calls for a fill When multiple freezers are connected to a common supply source it is advantageous to fill all freezers at the same time LN2 transfer losses are significantly reduced by filling all networked freezers while the supply system is primed and cold Using and OFAF network is more efficient than cooling the supply system every time an individual freezer fills This approach is also more efficient than employing a keep full keep cold system Up to 100 Controllers can be connected to an OFAF network An OFAF network can be configured in two modes Sequential or Simultaneous For both modes when any controller in the network initiates a fill the Master controller OFAF ID 1 recognizes this and triggers all other controller to initiate fills as well In Sequential OFAF mode once the controller that initiated the first fill has reached its High Level Setpoint the Master will trigger the freezer with the next sequential OFAF ID to fill until it reaches its High Level Setpoint The Master will then trigger the freezer with the next sequential OFAF ID to fill and this process will continue until all freezers in the network including the Master have reached their High L
50. Menus 3 Press SETUP Until the display reads Password Menus 4 Press ENTER The display will read Password Entry Mode 5 Press SETUP The display will read Change Global Password The Global Password has Security Level 4 clearance 6 Press ENTER The display will read Global Password Use the A keys to input new Global Password and press ENTER to advance the cursor 7 Press ENTER The display will read Confirm new Password To confirm new password use keys to change from NO to YES and press ENTER to save 69 of 133 Higher User Level Required use A V to TAa A Press ENTER for Temperature Menus Press ENTER for Password menus Password Entry Mode ENABLED Press ENTER to Change global Global Password Use A Y to adjust Confirm new Password 326636H01 Rev 7 2 6 3 Multilevel Passwords This section describes how to setup and change the 9 multilevel passwords 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP Until the display reads Password Menus 4 Press ENTER The display will read Pas
51. NOTE Security Level 3 or higher is required to change the Communication Settings 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER P ENTER f The display will read Temperature Menus u Temperature Menus 3 Press SETUP P ENTER f Until the display reads Advanced Settings cu or Advanced Settings 4 Press ENTER Press ENTER for The display will read Advanced Fill Menus Advanced Fill menus 5 Press SETUP ENTER f Until the display reads COM 1 Menus For COM 2 Pe B Menus press SETUP 1 menus 6 Press ENTER COM 1 Set The display will read COM 1 Setup Use the A Y ae keys to adjust and press ENTER to save 9600 N81 7 Press SETUP COM 1 T The display will read 1 Type Use the A Y 9 keys to adjust and press ENTER to save ASCII 60 of 133 326636H01 Rev A 7 2 5 3 3 MODBUS ID The Controller MODBUS ID also serves as the ASCII Unit ID This address can be adjusted in the MODBUS Menu and has a range of 1 to 200 NOTE Security Level 2 or higher is required to change the MODBUS ID 1 Press SETUP Controller will prompt for a password A flashing cursor on the e
52. NTER to save Press SETUP The display will read Year Use the A keys to adjust the year and press ENTER to save Press SETUP The display will read Month Use the A Y keys to adjust the month and press ENTER to save 10 Press SETUP The display will read Day Use the A V keys to adjust the day and press ENTER to save 11 Press SETUP The display will read Time Format Use the A V keys to adjust and press ENTER to save 58 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Advanced Settings Press ENTER for Advanced Fill menus Press ENTER for Set Time and Date Hour 12 00 Minute 12 31 Year 2009 Month 10 Day 22 Time Format 12 31 PM 326636H01 Rev A 12 Press SETUP The display will read Date Format Use the A Y keys to adjust and press ENTER to save MM DD YY Date Format 59 of 133 326636H01 Rev 7 2 5 3 1 Communication Settings 7 2 5 3 2 Setup Type The settings for the two serial ports can be adjusted in the COM 1 and COM 2 Setup Menus Since they are independent serial ports only one serial port can be set to a given COM Type If COM 1 is set to ASCII then COM 2 cannot also be set to ASCII Networked controllers must be daisy chained together with RJ 45 splitters in order to communicate using the same COM Type
53. OINT Place Probe A in il Use adjust 2 and press time out Press ENTER for next Cabieation aborset Remove Probe A fram LN2 and press ENTER Wait while probe warms 50 temperature 180 seccads calibration complete 96 of 133 326636H01 Rev A Calibration Type SINGLE POINT Use 11 to adjust Press ENTER for next Calibration complete Remove Probe B from LN2 and press ENTER Wait while pmbe warms 0 mom temperature 180 seconds Place Probe B in water and press ENTER 8 4 Press ENTER tor Lid Switch menus Press ENTER tor Add on nems press SETUP toe next men ENTER Batiory Status MVDC Sower Fakre Alarm DEABLED Use 1 1 tosdist Peon ENTER to ese Lid Switch installed NO Use T 1 Prom ENTER do vn Lid Open Fog DISABLED Uso 1 1 Prom ENTER to cue bid Open Alarm Time 1mn Use T 1 toadiuet Prem ENTER to ena Bypass Alarm DBAELED Use T 1 toodet Press ENTER to Bypass Temperature aoc Bypess T emp Setpart 5 oc Use T 1 Press ENTER to cave Bypass Alam Tene Delay 5Mnutes Use to edant Preca ENTER 1020 Stuck Valve Alarme DEMRED Use 1 1 Press EXTER to sum Stuck Imn Use T 1 vadit Press ENTER to eme Stuck Closed Delay Imn Use T 1 Prose ENTER to savo Press ENTER Cmibratonor pre
54. R The display will read Timed Fill 6 Press SETUP Until the display reads Maximum Fill Time Use the keys to adjust and press ENTER to save 57 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Advanced Settings Press ENTER for Advanced Fill menus Timed Fill DISABLED Maximum Fill Time 60 min 326636H01 Rev 7 2 5 3 Time and Date The time and date can be set in the Advanced Settings Menus The user has the option of a 12 or 24 hour clock and a MM DD YY or DD MM YY date format NOTE Security Level 2 or higher is required to change the time and date Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Press ENTER The display will read Temperature Menus Press SETUP Until the display reads Advanced Settings Press ENTER The display will read Advanced Fill Menus Press SETUP The display will read Set Time and Date Press ENTER The display will read Hour Use the keys to adjust the hour and press ENTER to save Press SETUP The display will read Minute Use the keys to adjust the minute and press E
55. SCII command window automatic COM Port selection real time temperature level monitoring and the event log download button that allows a date range selection It will include the fill control buttons and a quick parameter setup table For additional information refer to the Controller Connect user manual Status COMIS connected Required Items e Microsoft Windows Vista XP WIN7 e Controller COM USB Kit P N 13376947 e USB Port 85 of 133 326636H01 Rev A Installing Controller COM USB Kit 1 Insert the included USB to Serial Driver Disc into the PC CD ROM 2 Plug the Controller COM USB Kit COM USB Kit into an open USB port 3 Follow the automatic installation prompts Downloading Controller Connect 1 Download latest version online 2 Click the link and open the compressed folder 3 Extract all files from the compressed folder to a location on your hard drive 4 Open the extracted SETUP file 5 Follow the prompts to complete installation 6 A Controller Connect icon should appear on your programs list when complete Connecting to Controller Controller Settings 1 COM Setup 9600 N81 2 COM Type ASCII 3 Select a unique MODBUS ID Unit ID for each controller involved Controller Connect Settings Connect Controller serial port to PC with Controller COM USB Kit Open Controller Connect Determine the Windows COM Port by clicking Find COM Port on the Controller Connect main page Note the COM nu
56. TB LOTB LOTBS LOTBM LOTBM 326636H01 Rev A Set Temp A Low Alarm Input LOTA zXXX X Returned No response Comments Used to set the Temp A Low Alarm value Temp A Low Alarm Query Input LOTA Returned XXX X Comments Used to query the Temp A Low Alarm value Temp A Low Alarm Status Query Input LOTAS Returned 0 or 1 Comments Used to query the Temp A Low Alarm status where 0 off and 1 on Set Temp A Low Alarm Mask Input LOTAM 0 or LOTAM 1 Returned No response Comments Used to enable or disable the Temp Low Alarm where 0 disabled and 1 enabled Default setting is 1 enabled Temp A Low Alarm Mask Query Input LOTAM Returned 0 or 1 Comments Used to query the Temp Low Alarm Mask status where 0 disabled and 1 enabled Default setting is 1 enabled Set Temp B Low Alarm Input LOTB zXXX X Returned No response Comments Used to set the Temp B Low Alarm value Temp B Low Alarm Query Input LOTB Returned XXX X Comments Used to query the Temp B Low Alarm value Temp B Low Alarm Status Query Input LOTBS Returned 0 or 1 Comments Used to query the Temp B Low Alarm status where 0 off and 1 on Set Temp B Low Alarm Mask Input LOTBM 0 or LOTBM 1 Returned No response Comments Used to enable or disable the Temp B Low Alarm where 0 disabled and 1 enabled Default setting is 1 enabled Temp B Low Alarm Mask Query Input LOTBM Returned
57. TER for next Level 1 Password 6 level Use 1 10 adjust Press ENTER for next Level 1 Password 7 level Use 1 10 adjust Press ENTER far next Level 1 Password amp level Use 1 10 adjust Press ENTER far next Level 1 Password 9 level Use 1110 adjust Press ENTER far next Level 1 Press ENTER for nex XXXX Password 1 Press ENTER tn Use 11 to adjust Change Password 1 Press ENTER for nex XXXX Password 2 Use 11 to adjust Press ENTER for nex XXXX Password 3 Use 11 to adjust Change Password 3 Press ENTER for nex XXXX Password 4 Use 7 10 adjust Change Password 4 Press ENTER for nex or press Setup for Password 5 Use to adjust Change Password 5 Press ENTER for nex Password 6 Use 7 10 adjust Change Password amp Press ENTER for nex or press Satup for XXXX next Password 7 Use 1 10 adjust Press ENTER for nex XXXX Password 8 Use 11 10 adjust Change Password 8 Press ENTER for next or press Setup for XXXX next Password 9 Use 11 10 adjust Press ENTER for nex XXXX 103 of 133 Press ENTER for Password menus or press SETUP for next menu ENTER Security Level 4 Password Entry Mode ENABLED Use 11 to adjust Press ENTER to 326636H01 Rev A 8 11 Controller Display Screens Main Display Filling Display Temp A 200 0 C Temp 200 0 C Tene B 200 0 Level foo Level 10 0in Usage 12 4 inday FILUNG MM 5S Bypass
58. Thaw and Obstruction in level sensing line Insufficient venting Replace gasket lid if 111 necessary Introduction retrieval of racks or samples Liquid usage value should return to normal as more data is Lid open for extended period of acquired High liquid usage time Inadequate LN2 supply AIR LN2 nu LN2 Supply Purge level sensing annular line and clear any debris in the bottom of Moisture Removal freezer Complete freezer Page 112 thaw and moisture removal 9 may be necessary Consistently high and increasing Contact your authorized CryoExtraTM Distributor or Technical Service liquid usage _ _ _ Complete Freezer Thaw Obstruction in level sensing line 116 of 133 326636H01 Rev A 11 EN Compliance Tables Table 1 Guidance and manufacturer s declaration electromagnetic emissions for all Controllers See 5 2 2 1 C Guidance and Manufacturer s Declaration Electromagnetic Emissions The CONTROLLER is intended for use in the electromagnetic environment specified below The customer or the user of the CONTROLLER should assure that it is used in such an environment Flicker emissions IEC 61000 3 3 Emissions Test Compliance Electromagnetic Environment Guidance RF emissions Group 1 The CONTROLLER uses RF energy only for its internal functions CISPR 11 Therefore its RF emissions are very low and are not lik
59. Using a 9 16 or small adjustable wrench remove the sensor from the plumbing assembly It may be necessary to temporarily disconnect the sensor from the wire harness to avoid over twisting of the wires Following removal of the sensor reconnect the sensor wires 5 Perform the hot gas bypass sensor calibration procedure 6 Following calibration reinstall the bypass sensor using new PTFE thread tape ensure sensor wires are connected reinstall plumbing shroud or rear panel and reconnect the LN2 supply source 80 of 133 7 3 3 1 Hot Gas Sensor Single Point Calibration 1 Press SETUP Controller will prompt for a password A flashing cursor will make it clear which digit is being changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Press ENTER The display will read Temperature Menus Press SETUP Until the display reads Add on Menus Press ENTER The display will read Battery Status Press SETUP Until the display reads Hot Gas Bypass Menus Press ENTER The display will read Hot Gas Bypass Press SETUP Until the display reads Bypass Probe Calibration Press ENTER The display will read Calibration Type Use the A V keys to set as SINGLE POINT 9 Press ENTER The display will read Bypass Calibration Submerge the sensing end of the bypass probe in LN2
60. al between each level setting and alarm High Level Alarm Query Input HILA Returned XXX X Comments Used to query the High Level Alarm value Value returned in either inches or millimeters depending on the current level units There must be at least a 0 5 inch interval between each level setting and alarm High Level Alarm Status Query Input HILS Returned 0 or 1 Comments Used to query the High Level Alarm Status where 0 off and 1 on Set High Level Alarm Mask Input HILM 0 or HILM 1 Returned No response Comments Used to enable or disable the High Level Alarm where 0 disabled and 1 enabled Default setting is 1 enabled 129 of 133 HILM LEVL LFIL LFIL LOLA LOLA LOLS LOLM 326636H01 Rev A High Level Alarm Mask Query Input HILM Returned 0 or 1 Comments Used to query the High Level Alarm Mask status where 0 disabled and 1 enabled Default setting is 1 enabled Current LN2 Level Query Input LEVEL Returned XXX X Comments Used to query the current LN2 Level reading Set Low Level Setpoint Input LFIL XXX X Returned No response Comments Used to set the Low Level Setpoint Input value uses the current level units either inches or millimeters There must be at least a 0 5 inch interval between each level setting and alarm Low Level Setpoint Query Input LFIL Returned XXX X Comments Used to query the Low Level Setpoint Value returned in either
61. annel 75 of 133 326636H01 Rev Level dip stick inserted to measure the physical liquid nitrogen level Caution Picture is for demonstration only on an empty tank Please use protective gear while working with liquid nitrogen Please refer Section 3 for Safety while working with Liquid nitrogen 76 of 133 326636H01 Rev Liquid Level Calibration NOTE Liquid level calibration cannot be performed while the Controller is filling If Controller is filling press Stop Fill and perform the calibration Allow freezer plumbing to thaw 10 to 15 minutes before calibrating NOTE Liquid level calibration is most accurate when calibrated at 10 0 inches 254 mm Calibration must be performed above 3 0 inches 75 mm NOTE Security Level 3 or higher is required to calibrate the liquid level 1 Press SETUP Higher User Level Controller will prompt for a password A flashing cursor will make it clear which digit is being changed Use the Required use A Y to keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER P ENTER f The display will read Temperature Menus Mod P Temperature Menus 3 Press SETUP NT Press ENTER for Until the display reads Liquid Level Menus Liquid Level menus 4 Press ENTER High Level AI The display will read High Level Alarm 8 0 5 Press SETUP Press ENTER to Until the d
62. ated alarm is cleared Press Alarm Mute NOTE The heat generated by the sensor during the High Temperature Alarm Test is NOT enough to affect the actual temperature in the freezer storage space If the sensor is submerged in LN2 the heat generated by the sensor may not be sufficient to initiate a high temperature alarm 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press ENTER The display will read A Menu NOTE To access Temp B Menu press SETUP instead of ENTER 4 Press ENTER The display will read Temp A Enabled 5 Press SETUP The display will read Initiate High Temp A Alarm Test Use the A V keys to change NO to YES and press ENTER to initiate the alarm test If High Temp Alarm Test is initiated the display will read Testing and show the temp change throughout the test Note If the temperature probe is open or no probe is connected the controller will not go into the high temperature alarm test mode 40 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Temp A Menu Temp ENABLED Initiate High Temp A Alarm Test In
63. ated on the unit either audibly or visually It will only be recorded in the internal data log This is to minimize self correcting nuisance warnings A New menu page was added in the Display and Output menu after the Enable Liquid Usage Display page to allow enabling disabling the liquid usage alarm 51 of 133 326636H01 Rev 7 2 4 3 Alarm Buzzer The audible alarm buzzer can be disabled This will not disable the alarms only the audible buzzer Alarms will still be displayed visually on the screen if the alarm buzzer is inactive NOTE Security Level 2 or higher is required to enable disable the alarm buzzer 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER P ENTER f The display will read Temperature Menus box t Temperature Menus 3 Press SETUP Press ENTER for Until the display reads Display and Output Display and Output 4 Press ENTER Temperature Units The display will read Temperature Units 8 5 Press SETUP P ENTER f Until the display reads Advanced Display and Output ics ue Advanced Display 6 Press ENTER The display will read Alarm Buzzer Use the A V keys to
64. attery Backup 15 pin AMP Serial Ports RJ 45 4 pin RS 485 Temperature Sensor Type 2 wire Platinum RTD Pt 1000 Quantity 2 Resistance 1000 0 Sensitivity 3 85 C Temperature Measurement Resolution 0 1 C 0 2 F Accuracy Single Point Calibration Two Point calibration 1 0 C 1 8F 2 0 3 6 Range 200 C to 70 C 328 C to 158 F Level Measurement Type Differential Pressure Sensor Accuracy 0 5 in 13 mm LN2 Resolution 0 1 in 2 5 mm Range 3 0 in to 48 in 76 mm to 1219 mm RevA Accuracy determined over range of 200 C to 135 C Accuracy decreases slightly as range increases Accuracy determined over a range of 200 C to 0 C Accuracy decreases slightly as range increase 16 of 133 326636H01 Rev A 5 6 Operating Environment Ambient Temperature and Relative Humidity CryoExtra cryogenic freezers are designed to be operated in environments near room temperature 65 F 80 F 18 27 C and relative humidity below 50 Due to the large gradient between LN2 and ambient temperatures an additional change of a few degrees will not have a significant impact on the freezer performance Although temperature changes will affect the top temperatures during normal operation and lid opening to a greater degree it again will not be a significant effect The relative humidity should be maintained low enough so that condensation does not form on t
65. awback is a longer more complex calibration procedure The benefit of a single point calibration is a simple calibration procedure The drawback of single point calibration is less accurate temperature measurement in near room temperature environments All new freezers equipped with controllers have been calibrated at the factory The temperature sensors should only be calibrated if faulty readings are suspected a sensor or the Controller itself has been replaced following a firmware update or as a part of a preventative maintenance schedule For an accurate calibration the LN2 Saturation Temperature Section 7 2 1 4 needs to be correctly set based on the altitude of the freezer location NOTE Security Level 2 or higher is required to calibrate temperature sensors CAUTION Always wear protective gloves and face shield when handling LN2 Refer to the Safety section of this manual 71 of 133 7 3 1 1 Single Point Calibration The single point calibration procedure requires a small volume of LN2 enough to completely submerge the end of the temperature sensor It may be possible to use the LN2 in the freezer space if the probe length permits 1 Press SETUP Controller will prompt for a password Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press ENTER The display will rea
66. bypass time If the temperature in the plumbing system does not reach the Bypass Temperature Setpoint within the Bypass Alarm Time Delay setting the Controller will terminate the bypass cycle initiate the fill cycle and trigger a Hot Gas Bypass Time Alarm Once the immediate fill cycle is complete auto fill will be disabled until the Hot Gas Bypass Time Alarm is acknowledged This feature prevents a freezer from continuing to attempt to fill from an empty supply A Hot Gas Bypass Time Alarm could be a sign of an empty supply a supply with too low of pressure or it could mean that the Bypass Alarm Time Delay needs to be increased in order to effectively purge the supply line of nitrogen gas The default Bypass Alarm Time Delay is 5 minutes but should be adjusted based on the freezer setup It should be arranged so that the Bypass Temperature Setpoint is easily reached within the Bypass Alarm Time Delay setting NOTE If a Hot Gas Bypass Time Alarm is triggered once the immediate fill cycle is complete auto fill will be disabled until the alarm is acknowledged 36 of 133 326636H01 Rev A 7 1 12 Battery Backup Optional The Battery Backup comes standard on all MDD freezers The Controller is able to run seamlessly on power from this external battery system when the primary power source is interrupted The Controller is able to run fully functional on the Battery Backup for approximately 72 hours in the event of a power failure While the Con
67. connect the LN2 supply If using an LN2 cylinder as the liquid supply securely connect and tighten the transfer hose to both the fill tee connection and the supply connection labeled LIQUID If using a bulk LN2 supply System securely connect and tighten the supply connection to the freezer fill tee connection NOTE The recommended LN2 supply pressure is 22 35 psig 1 52 2 41 bar First Fill Startup procedure 1 2 Once the freezer has been properly installed begin the first fill procedure Fill freezer at the desired location where it is to be used Do not fill to move freezer to another location Open or remove the lid for the first fill due to the accelerated LN2 evaporation rate when filling a warm freezer If the freezer is equipped with a lid switch engage the manual override see the Lid Switch section of this manual for more info Verify supply pressure at 22 35 psig 1 52 2 41 bar open valve and press Start Fill to begin filling the freezer The first fill will take significantly longer than subsequent fills due to a warm freezer It is recommended to place empty inventory system components such as racks boxes frames or canisters in the freezer during the first fill This will cool the inventory system as well as help the freezer reach its top box temperature rating faster It is normal for some condensation or frost to develop around the neck opening during the initial fill Once the Controller reads a l
68. connections with leak detect solution 2 Allow leak detect solution to penetrate fittings for at least 30 seconds 3 Large leaks will be immediately apparent with large bubble formations 4 Small leaks will take longer to detect with small bubble formation in the appearance of foam 5 Most leaks can be repaired by tightening the suspect fitting with a crescent or appropriate sized wrench 6 If tightening the fitting does not fix the leak check the fitting for cracks and or galling If the fitting is damaged replace 7 Recheck any replaced fittings for leaks High Temp Alarm Test Please refer to Section 7 2 1 2 for details and instructions on the High Temp Alarm Test 107 of 133 326636H01 Rev Level Alarm Test The Controller can trigger a high level or low level alarm if the LN2 level in the freezer exceeds the user defined parameters High Level Alarm Test 1 Observe and record the current LN2 level Record the current level settings They may be accessed by pressing the and simultaneously to access the quick reference menu or through the Liquid Level Menus The current LN2 level should be between the High Level Alarm setting and Low Level Alarm setting If not allow the freezer to fill until it reaches the High Level Fill setting 3 Adjust the level offset so that the current level is a value that is greater than the High Level Alarm setting For example if the High Level Alarm setting is current
69. d Temp A Menu Press SETUP for Temp B 4 Press ENTER The display will read Temp 5 Press SETUP Until the display reads Temp A Calibration 6 Press ENTER The display will read Calibration Type Use the A V keys to adjust to SINGLE POINT 7 Press ENTER The display will read Temp A Calibration Completely submerge the sensing end of Temp probe A in LN2 8 Press ENTER The display will read Wait for Temp A to stabilize Wait for the displayed Temp A reading to stabilize while the probe is submerged in LN2 before pressing ENTER 9 Press ENTER The display will read Probe A single point calibration complete 72 of 133 326636H01 Rev A Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Temp A menu Temp ENABLED Press ENTER to Temp A Calibration Calibration Type SINGLE POINT Temp A Calibration Place Probe A in Wait for Temp A to stabilize then press Probe A single point calibration complete 326636H01 Rev A 7 3 1 2 Two Point Calibration The two point calibration procedure requires a small volume of LN2 and an ice water bath enough to completely submerge the end of the sensor Proper ice water bath preparation is imperative to ensure accuracy It is best to add filtered water to a Styrofoam cup containing crushed ice Allow the solution to s
70. download the event log the controller s unit ID and the controller s firmware version The events are logged so that Record 1 is the most recent event logged The event parameters are logged in the units and format of that respective category For example if the Controller temperature units are set to be in degrees Celsius then the temperature will be logged in degrees Celsius If the time is set to a 24 hour clock then the event log times will be in that format Parameter changes will be logged as a string such as Parameter number 126 changed from 60 to 180 Contact your CryoExtra Distributor or CryoExtra Technical Service for information regarding these parameter changes ControllerConnect v1 1 2 CryoExtra Controller Software ver 2 00 Record UnitID Date Time TempA TempB LN2 Level LN2Usage Event Codes 1 200 12 15 10 9 00 186 7 194 8 6 1 0 5 AH 2 200 12 15 10 5 00 191 4 195 8 6 3 0 5 3 200 12 15 10 1 00 191 4 195 8 6 5 0 5 4 200 12 15 10 00 00 191 4 195 8 6 6 0 5 ZO 5 200 12 14 10 21 00 191 4 195 8 6 6 0 5 6 200 12 14 10 17 00 191 4 195 8 6 8 0 5 7 200 12 14 10 9 00 191 4 195 8 6 8 0 5 8 200 12 14 10 7 15 191 5 195 8 7 0 0 0 9 200 12 14 10 6 32 191 5 195 8 5 0 0 0 10 200 12 14 10 5 00 191 5 195 8 5 3 0 0 Figure 9 Sample event log download 33 of 133 TEMPERATURE 326636H01 Rev Table 4 Controller Event Log Codes
71. e a user attempts to access the setup menus A flashing cursor on the password entry screen will make it clear which digit is being changed Feature is available with Firmware Version 2 02 These instructions will start from the main monitoring display screen assume password entry mode is enabled and the user has Security Level 4 clearance Once in the setup menus the user can press the ESC key to exit that menu level or press the ESC key repeatedly until the display returns to the main monitoring screen After 30 seconds of inactivity the display will automatically return to the main monitoring display screen Controllers with newer displays have the added functionality of a quick reference scroll menu Pressing the up and down arrow keys simultaneously while on the main monitoring screen will display the controller s serial number and firmware version Pressing the up or down arrow keys will then scroll through the level temperature and gas bypass settings The user can return to the main monitoring screen by pressing the ESC key or waiting 30 seconds Main Monitoring Display Screen 38 of 133 7 2 1 Temperature Settings 326636H01 Rev A This section describes how to access and adjust the various temperature settings on the Controller NOTE Security Level 2 or higher is required to adjust the Temperature Settings 7 2 1 1 Enable Disable Sensors The default setting for both Temp A and Temp B sensors is enabled
72. e far end of the network from the LN2 supply 2 Connect one end of the OFAF Master Cable to either Serial Port 1 or 2 of the Master controller NOTE Either Serial Port 1 or 2 can be used for OFAF as long as the COM Types are in agreement 3 Connect an RJ 45 splitter to the next controller in the network Connect the free end of the OFAF Master Cable to this splitter 4 Set the OFAF ID of this second controller to 2 5 Connect an RJ 45 splitter to the third controller in the network Connect the splitters on the second and third controllers with a standard CAT 5e cable 6 Set the OFAF ID of the third controller to 3 7 Continue this pattern until all controllers in the network are daisy chained and assigned sequential OFAF IDs 8 Each controller in the network should also have the following settings a Auto Fill Control Enabled b COM Setup 9600 N81 COM Type OFAF d OFAF Type Sequential or Simultaneous All controllers in a network must have the same OFAF Type e OFAF Units of controllers 1 The Master controller is not counted in the number of OFAF Units All controllers in a network must have the same number of OFAF Units 90 of 133 326636H01 Rev Cable Wiring OFAF Master Cable Figure 18 OFAF Master Cable wiring configuration Numbering as viewed from side of RJ 45 connector opposite latch Standard Cable
73. e that can be opened in EXCEL Either a single controller or a network of controllers may be connected to a host computer For more information on computer communication capabilities refer to the Communication Networking section e Serial Printer The Controller can be connected directly to a serial printer via an RS 485 converter This allows controller events to be printed as they occur This gives users the opportunity to keep hard copy records of the freezer s status in addition to the standard event log saved in the controller s memory This printed data bypasses the controller memory and is not given the opportunity to be altered electronically For more information on the printer interface refer to the Communication Networking section e MODBUS The Controller has RS 485 MODBUS communication capabilities This will not be extensively covered in this manual Please contact your CryoExtra Distributor or CryoExtra Technical Service for more information e One Fill All Fill OFAF A group of Controllers can be networked in order to coordinate fill cycles and reduce LN2 transfer losses For locations with multiple freezers this function will increase the filling efficiency and drastically reduce LN2 consumption over time A sequential or simultaneous OFAF network is possible For more information on OFAF networking refer to the Advanced Filling Options section 32 of 133 326636H01 Rev 7 1 9 Event Log And Event Codes
74. el Setpoints and Alarms The auto fill settings can be adjusted in the Liquid Level Menus With auto fill enabled the Controller will initiate an auto fill when the level is at or below the Low Level Setpoint and terminate a fill when the level reaches the High Level Setpoint If the level is at or below the Low Level Alarm or at or above the High Level Alarm the Controller will initiate that respective alarm NOTE There must be a minimum of 0 5 inches between each of setpoint and alarm For example if the Low Level Alarm is set to be 4 0 inches then the Low Level Setpoint would have to be set at least 4 5 inches The Low Level Alarm can be set to a minimum value of 3 0 inches and the High Level Alarm can be set to a maximum value of 48 0 inches NOTE Security Level 2 or higher is required to adjust the Liquid Level Settings 1 Press SETUP Controller will prompt for a password A flashing cursor moner ser evel on the entry screen will make it clear which digit is being Required use A Y to changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER Press ENTER for The display will read Temperature Menus Temperature Menus 3 Press SETUP ENTER Until the display reads Liquid Level Menus ress or Liquid Level menus 4 Press ENTER High Level Al The display will read High Level Alarm Use the A Y AUSSIE E
75. ell ventilated If inhaled move to fresh air If breathing is difficult supplemental oxygen may be required If not breathing give artificial respiration SEEK MEDICAL ATTENTION IMMEDIATELY Liquid nitrogen can cause severe frostbite to the eyes or skin DO NOT touch frosted pipes or valves Cold nitrogen vapor can damage the eyes or skin In case of frostbite consult a physician at once If a physician is not readily available warm the affected area with water that is near body temperature Never place LN2 in a sealed container without a pressure relief device The expansion ratio of liquid nitrogen to gaseous nitrogen is approximately 1 to 700 i e 1 cubic foot of LN2 becomes 700 cubic feet of gas when evaporated The two most important safety aspects to consider when handling LN2 are adequate ventilation and eye and skin protection Although nitrogen gas is non toxic it is dangerous in that the gas will displace oxygen in a normal breathing atmosphere Liquid products are of even greater threat since a small amount of liquid evaporates into a large amount of gas Therefore it is imperative that cryogenic supply and storage Dewars be stored and operated in well ventilated areas 7 of 133 326636H01 Rev Persons transferring LN2 should make every effort to protect the eyes and skin from accidental contact with liquid or cold vapor ThermoFisher Scientific recommends the following protective clothing and accessories when transferring
76. elow 50 PSI 3 4 bar until the line in clear and completely free of any moisture NOTE If moisture is not completely removed for the freezer space and annular lines ice will form when LN2 is reintroduced into the freezer Ice blockage in the freezer space or annular lines will interfere with proper function of the freezer and level sensing system ENSURE ALL MOISTURE IS COMPLETELY REMOVED PRIOR TO INTRODUCING LN2 113 of 133 326636H01 Rev 9 3 Replacement Parts and Accessories Replacement Parts Part Number Description 11648945 Inline Filter 40 micron mesh 1810032 Relief Valve 50 PSI 3 4 bar 142246115 SMC Solenoid Valve Fill and Gas Bypass 132849545 Purge 3 way Solenoid Valve 10713400 Gas Bypass Temperature Sensor Pt 1000 RTD 11499812 Gas Bypass Muffler 11885449 Gas Bypass Muffler Deflector 20570663 Temperature Probe All CryoExtra Series 65 14248744 HE Series 3 Tube Temperature Sensor Assembly 26 inch 660 mm 14248816 HE Series 3 Tube Temperature Sensor Assembly 39 inch 990 mm 14248752 HE Series 3 Tube Temperature Sensor Assembly 44 inch 1118 mm 11795030 Jerome Power Supply Input 110 230 VAC Output 30 VDC AC cord not included 14010103 Power Outlet Cord 110 VAC The Americas 10995363 Power Outlet Cord 230 VAC Europe 10718155 Battery Backup Replacement Battery 12 VDC 11858467 Battery Backup
77. ely to cause any interference in nearby electronic equipment RF emissions Class B The CONTROLLER is suitable for use in all establishments CISPR 11 including domestic establishments and those directly connected to the public low voltage power supply network that supplies Harmonic emissions Class A buildings used for domestic purposes IEC 61000 3 2 Voltage fluctuations Complies 117 of 133 Table 2 Guidance and manufacturer s declaration electromagnetic immunity for all Controllers See 5 2 2 1 326636H01 Rev Guidance and Manufacturer s Declaration Electromagnetic Immunity The CONTROLLER is intended for use in the electromagnetic environment specified below The customer or the user of the CONTROLLER should assure that it is used in such an environment Immunity Test EN60601 1 2 Test Level Compliance Level Electromagnetic Environment guidance Electromagnetic Discharge ESD IEC 61000 4 2 6 kV contact 8 kV air 6 kV contact 8 kV air Floors should be wood concrete or ceramic tile If floors are covered with synthetic material the relative humidity should be at least 3096 Electrical fast 2 kV for power 2 kV for power Mains power quality should be that of a transient burst supply lines supply lines typical commercial or hospital IEC 61000 4 4 1 kV for input output 1 kV for environment lines input output lines Surge t1 kV line s to
78. emperature Menus 3 Press SETUP ENTER f Until the display reads Display and Output fess or Display and Output 4 Press ENTER The display will read Temperature Units Use the keys to adjust and press ENTER to save Temperature Units 5 Press SETUP P ENTER f The display will read Level Display Menus Level Display Menus 6 Press ENTER Level Unit The display will read Level Units Use the A Y keys ics to adjust and press ENTER to save in 7 Press SETUP Define 100 The display will read Define 100 If using the nds percentage level units then use the keys to 8 0 in adjust and press ENTER to save 8 Press SETUP Define 0 The display will read Define 0 If using the SARS percentage level units then use the A keys to 0 0 in adjust and press ENTER to save 50 of 133 326636H01 Rev A Percentage Level Units Explanation The defined 096 and 10096 values are independent of the automatic level control parameters The percentage level units are just an alternative to displaying the measured level in inches or millimeters The percentage parameters can be set to match the high and low fill setpoints or they can be set to any other value that makes interpreting the liquid level easier for the user 7 2 4 2 Liquid Usage Display NOTE Security Level 1 or higher is required to e
79. ent Log 34 of 133 326636H01 Rev 7 1 10 Lid Switch The Controller has the ability to support a lid switch This additional feature allows the user to disable filling while the lid is open utilize the Lid Open Alarm or initiate an automatic fog clear A lid switch comes standard on all CryoExtra Series There are two configurations of the lid switch the standard UL lid switch configuration and the optional fog clear lid switch configuration The difference in their functionality is based on how they are wired to the Controller Standard UL Lid Switch configuration The UL lid switch configuration which is standard on CryoExtra Series is wired directly in series with the fill valves With this configuration when the lid is open and the lid switch is disengaged the fill valves cannot be energized When the lid is down and the lid switch is engaged the fill valve circuit is closed and the valves can be energized The functionality of the UL lid switch is independent of the settings in the lid switch menu of the Controller since it is hardwired into the fill valve circuit The UL listing stipulates that the models must have the lid switch hardwired in series with the fill valves when it leaves the factory to be compliant The fog clear and lid open alarm features are not available with the UL lid switch configuration It is available for users to easily adapt their UL lid switch configuration toa fog clear lid switch configuration
80. er User Level Required use 7 1 to ENTER Passe od incorrect Password ENTER assword Loss of communications from the control board to the display English Only Communicabons Loss anroller 326636H01 Rev A 9 Preventative Maintenance 9 1 Preventative Maintenance Schedule This section describes the preventative maintenance that should be performed on CryoExtra freezers to ensure optimum operation and performance as well as maximum service life As with any technical piece of laboratory equipment preventative maintenance is key to equipment success NOTE This is the CryoExtra recommended preventative maintenance CryoExtra Distributors may have a more comprehensive maintenance service plan At the minimum the below schedule should be followed Table 8 Periodic Preventative Maintenance Schedule Weekly Monthly 6 Months 12 Months 24 Months 60 Months Level Verification X Verify Adequate Supply X Plumbing Leak Check X High Temp Alarm Test X Level Alarm Test X Thaw Freezer Lid X Folding Step Inspection X Lid Hinge Inspection X Inline Filter Replacement X Complete Function Test X Solenoid Valve Replacement X Relief Valve Replacement X Lid gasket replacement X Complete Freezer Thaw and X Moisture Removal 105 of 133 326636H01 Rev A 9 2 Preventative Maintenance Procedures
81. evel Setpoints In Simultaneous OFAF mode when any controller initiates a fill and fills for at least 60 seconds the Master will then broadcast a signal for all freezers including itself to begin filling Each freezer will continue to fill until its High Level Setpoint is reached A user would select sequential OFAF over simultaneous if their supply system is not able to maintain the proper filling pressure while multiple freezers filling at the same time Sequential OFAF allows freezers to fill one at a time with a primed and cold supply system so that it is easier for the system to maintain the proper filling pressure Sequential OFAF Simultaneous OFAF Master 2 OFAF3 OFAF4 Master OFAF2 OFAF3 OFAF4 82 Figure 16 Diagram showing differences between Sequential and Simultaneous OFAF Network Setup 89 of 133 326636H01 Rev OFAFID 1 OFAFID 2 OFAFID 3 OFAF ID 4 Serial Port 1 or2 RJ45 Splitter PN 10856312 Master Cable PN 11358251 Standard Cable PN 10740053 Figure 17 Diagram showing a typical OFAF network setup Table 7 Required items for OFAF network P N Description Quantity needed 11358251 OFAF Master Cable 1 10740053 Standard CAT 5e Cable of controllers 2 10856312 RJ 45 Splitter of controllers 2 1 Designate the Master controller by setting its OFAF ID to 1 Generally this is the controller on th
82. evel press stop fill allow liquid to equalize and insert dipstick verify level to the controller level display change the offset value or to match liquid level press start fill to continue the fill process When freezer has reached its high level set point wait 10 minutes for system to equalize then verify level with dipstick and calibrate if necessary Once the Controller matches freezer LN2 level close or place the lid and allow the freezer to equilibrate and reach its temperature rating It is recommended that biological samples are not introduced into the freezer until several days after the top box temperature has stabilized at or below the freezer s temperature rating It is recommended to restart controller after 24 hours of initial install to reset the Liquid Usage 10 Refer to the Operations section of this manual for further instructions Note Pressing Stop Fill will disable Controller s automatic fill function for 30 minutes 21 of 133 326636H01 Rev 7 Operation This section of the manual will detail the functions and features of the Controller and demonstrate how to access and adjust the various user settings and options All CryoExtra Freezers utilize LN2 as the means of refrigeration Under atmospheric conditions the temperature of LN2 is 196 C 320 F 77 Depending on the model the LN2 resides either or below the freezer storage space Through normal usage and time the LN2 will naturally boil of
83. ezer 10 52 Plumbing FASS ITU aceto ist tr t ETE Ebert tard itai eph 12 5 3 C ntroller D 13 5 4 Controller Stand Alone Back Panel Physical 14 5 4 1 Controller 12 Pin Wiring Harness Connections eese 15 3 of 133 326636H01 Rev Se CHIC ALON irc ET 16 5 6 Operating Environments ooo depen pt 17 6 Jnetallation anq Startup 18 NIE Go Dc RAE 22 Functions and FediUl es aset a 23 7 1 1 Liquid Nitrogen Level Measurement eese entente eterne 24 7 1 2 Automatic Liquid Nitrogen Level Control eese 25 7 43 Liquid Nitrogen js aqaa teet etae ttam Rn e RN 26 7 1 4 Temperature Measurermiefits eoe roro ee ri her a pa QS 27 445 User Defined Alaf MS sinesine enge aste o trate egg etn dep br ea DAS tux 28 7 6 Remote Alarm Monitoring 30 o PessworndsJ SOC oem a 31 7 1 8 Communication Networking Capabilities eene 32 7 19 Event Log And Event Codes oor eR pet e en RR PA a 33 11 10 MAGS WHC Mae eR e 35 Pr PA Hot GAS YASS eases cok alates 36 7 1 12 Battery
84. f reducing the amount of refrigerant in the freezer It is imperative that the LN2 level be properly maintained in order for the storage space temperature to be maintained This along with monitoring and recording temperature is the main function of the Controller a Figure 3 Illustration showing LN2 as the refrigerant Left LN2 below the storage space naturally boils off and cools the freezer Right LN2 resides in the freezer storage space 22 of 133 326636H01 Rev 7 1 Functions and Features The Controller utilizes a variety of functions and features that enables it to closely monitor and control the environment inside a cryogenic freezer This section will give an overview of the following features and their functionality Liquid Nitrogen Level Measurement Automatic Liquid Nitrogen Level Control Liquid Nitrogen Usage Temperature Measurement User Defined Alarms Passwords Security Communication Capabilities Lid Switch Hot Gas Bypass Battery Backup Optional 23 of 133 326636H01 Rev A 7 1 1 Liquid Nitrogen Level Measurement The LN2 level in the tank is determined through the use of a differential pressure sensor This sensor operates on the physical principle of hydrostatic head pressure The pressure generated by a column of fluid is proportional to the height or depth of the fluid column In this application the pressure generated by the LN2 at the bottom of the freezer will increase as the LN2 level
85. g LN2 Refer to the Safety section of this manual Dip Stick Procedure 1 Open or remove the freezer lid to access the interior storage space 2 Hold the meter dip stick vertically with the 0 0 inch end pointed down 3 Lower the meter dip stick into the LN2 at the bottom of the freezer Ensure the meter dip stick is vertical and touching the bottom of the inner Dewar Some LN2 boiling will occur around the meter dip stick a CryoExtra Freezers Insert meter dip stick into the rectangular channel on the turn tray in order to access the liquid below the tray 4 Leave the meter dip stick in the LN2 for approximately 5 seconds 5 Remove the meter dip stick from the liquid and immediately wave it back and forth in the air A distinct frost line will begin to develop as moisture in the air condenses on the meter dip stick predominately where it was submerged 6 Subtract 0 5 inches 13 mm from the observed frost line to account for the LN2 boiling up around the meter dip stick while it was submerged This resultant level measurement represents the actual liquid level inside the freezer Once you have obtained the measured level proceed to the liquid level calibration Figure11 Meter dip stick showing example level frost line The resultant measured level would be 5 5 inches after subtracting 0 5 inches from the frost line to account for the LN2 boiling Note LN2 liquid at or above turn tray height will rise higher in the dip stick ch
86. he controller Elevated humidity levels can lead to excessive condensation and frost on and around the lid In situations where the relative humidity is high and uncontrollable the lid should be routinely wiped dry to prevent ice formation Should significant ice formation develop thaw as necessary Refer to the Preventative Maintenance procedures for details Atmospheric Pressure CryoExtra cryogenic freezers are designed to be operated in environments with atmospheric pressure range of 8 2 psi 57 2 kPa to 14 7 psi 101 kPa Thermal Load Since CryoExtra Freezers use LN2 as the refrigerant and do not employ any type of mechanical refrigeration the thermal load will be negligible to negative 17 of 133 326636H01 Rev A 6 Installation and Startup This section will review the basic receiving installation and startup procedures for CryoExtra Freezers Always inspect the bill of lading for accuracy and external crate packaging for damage before accepting the shipment Included with each full auto freezer e Literature Packet o Controller Quick Start Reference Guide PN 326636H02 o Manual Freezer Status Log PN10936355 o Warranty Statement Registration Certificate Controller Packaged in box separately for CryoExtra models Transfer hose 6 ft Inside freezer CryoExtra Dipstick Inside freezer Desiccant bag To be removed and discarded Inside freezer Liquid Nitrogen handling instructions CryoE
87. hysically move sensors 24 of 133 326636H01 Rev 7 1 2 Automatic Liquid Nitrogen Level Control The Controller is equipped with a fully automated LN2 level control system This level control system is based on user defined parameters that can be adjusted to maintain a LN2 level in a freezer over a range of 3 0 inches 76 mm up to 48 0 inches 1219 mm Since this control system utilizes the differential pressure system described previously these parameters can be adjusted electronically using the Controller keypad or remotely through a networked computer This automatic fill control feature can be disabled Below is a brief explanation of the four user defined level control parameters For instructions on accessing and adjusting the level control settings see the Liquid Level Set points and Alarms section e High Level Alarm If the LN2 level in a freezer reaches or exceeds this setting a High Level Alarm will result This audible visual alarm will cause the High Level discrete contact and the global remote to switch to alarm state e High Level Setpoint When the LN2 level in a freezer reaches this setting with or without automatic fill enabled the controller will close the fill valves and terminate the fill e Low Level Setpoint When the LN2 level is at or below this setting with automatic fill enabled the controller will initiate a filling cycle e Low Level Alarm If the LN2 level in a freezer is at or below this set
88. ibration Page 75 Check clear vinyl tube connections and integrity Resistance Table LN2 Level Calibration Leak in level sensing line Perform leak test on purge valve vinyl tube and fittings Plumbing Leak Check Page 107 Purge valve defective Check resistance of purge valve 140 ohms Purge valve replacement Insufficient freezer venting Increase lid gasket vent Replace gasket lid if necessary Lid Gasket Replacement Page 111 Obstruction in level sensing line Purge level sensing annular line and clear any debris in the bottom of freezer Complete freezer thaw and moisture removal may be necessary Complete Freezer Thaw and Moisture Removal Page 112 Non uniform AC voltage dirty power Install uninterruptable power supply UPS Battery Backup or quality surge protector 115 of 133 326636H01 Rev Transition to generator power See Incorrect LN2 level Inaccurate level readings readings above Increase lid gasket vent Insufficient freezer venting Replace gasket lid if Seri Replacement necessary Short cycle fills Purge level sensing annular line and clear any debris in the bottom of Moisture Removal freezer Complete freezer P age 112 thaw and moisture removal may be necessary See Incorrect LN2 level Inaccurate level readings readings above increase lid gaske verni Lid Gasket Replacement Page Complete Freezer
89. inches or millimeters depending on the current level units There must be at least a 0 5 inch interval between each level setting and alarm Set Low Level Alarm Input LOLA XXX X Returned No response Comments Used to set the Low Level Alarm value Input value uses the current level units either inches or millimeters There must be at least a 0 5 inch interval between each level setting and alarm Low Level Alarm Query Input LOLA Returned XXX X Comments Used to query the Low Level Alarm value Value returned in either inches or millimeters There must be at least a 0 5 inch interval between each level setting and alarm Low Level Alarm Status Query Input LOLS Returned 0 or 1 Comments Used to query the Low Level Alarm Status where 0 off and 1 on Set Low Level Alarm Mask Input LOLM 0 or LOLM 1 Returned No response Comments Used to enable or disable the Low Level Alarm where 0 disabled and 1 enabled Default setting is 1 enabled 130 of 133 LOLM LUNI LUNI RATE 326636H01 Rev A Low Level Alarm Mask Query Input LOLM Returned 0 or 1 Comments Used to query the Low Level Alarm Mask status where 0 disabled and 1 enabled Default setting is 1 enabled Set Level Units Input LUNI E LUNI M or LUNI 96 Returned No response Comments Used to set the LN2 level units where E English inches M Metric millimeters and 96 Percent Level Units Query Input LUNI
90. ing and Sanitizing 133 5 of 133 326636H01 Rev Page intentionally blank 6 of 133 326636H01 Rev 3 Safety and First Aid READ BEFORE OPERATING THIS EQUIPMENT General Liquid nitrogen LN2 is used in CryoExtra Cryogenic Freezers as a refrigerant Understanding and following certain safety precautions is extremely important when handling LN2 and cryogenic containers Dewars Liquid Nitrogen Properties Nitrogen is a colorless odorless tasteless gas Gaseous nitrogen makes up about 78 of the Earth s atmosphere by volume Once collected and isolated nitrogen can be liquefied Boiling Point 1 atm 195 8 C 320 3 F 77 4 Thermal Conductivity Gas 25 83 mW m K Heat of Vaporization Liquid 198 38 kJ kg Density 1 atm Liquid 1 782 Ibs L 807 4 g L 808 6 kg m Liquid Nitrogen Safety Transferring LN2 and operating the Controller should be done in accordance with the manufacturer supplier instructions It is important that all safety precautions written on the storage Dewar and recommended by the manufacturer be followed ANWARNING Do not modify this equipment without authorization of the manufacturer Nitrogen is a potential asphyxiant and can cause rapid suffocation without warning Store and use in area with adequate ventilation DO NOT vent container in confined spaces DO NOT enter confined spaces where gas may be present unless area has been w
91. isplay reads Advanced Level Menus Advanced Level menus 6 Press ENTER Auto Fill Control The display will read Auto Fill Control ENABLED 7 Press SETUP Until the display reads Level Calibration Level Calibration Press ENTER to 8 Press ENTER The display will read Please wait while the sensor zeros Wait for the controller to count down from 60 seconds Please wait while the sensor zeros 77 of 133 326636H01 Rev A 9 Wait 60 seconds After the controller counts down the display will read Actual Level Use the A V keys to input the meter dip stick measured level Actual Level 10 0 in 10 Press ENTER Level Calibrati The display will read Level Calibration Complete MIL E Complete 11 Verify that the home screen reads the liquid level value that was just entered In some cases the controller should be restarted Contact Technical Support with any questions 78 of 133 326636H01 Rev A 7 3 3 Hot Gas Bypass Sensor Calibration This section describes how to calibrate the Controller hot gas bypass sensor There are two calibration procedures single point and two point calibration For single point calibration the reference point is LN2 For two point calibration the reference points are LN2 and ice water Unless regulations require a two point calibration the single point calibration procedure is recommended All new freezers ha
92. itiate High Temp A Alarm Test 326636H01 Rev A 7 2 1 3 Temperature Alarm Settings This section describes how to navigate and adjust the temperature alarm settings The high temperature alarm is used to alert the user when the temperature in the freezer space has risen above the set threshold The low temperature alarm can be used as a calibration error or redundant overfill indicator To be used as a calibration error indicator the low temperature alarm would be set to a temperature slightly colder than the LN2 saturation temperature Since the temperature in the freezer storage space can never be colder than LN2 if this alarm is triggered it is an indication that the sensor requires recalibration To be used as a redundant overfill or high level alarm the low temperature alarm would be set to a temperature slightly warmer than the LN2 saturation temperature and the sensor placed higher than the desired liquid level in the freezer If the low temperature alarm is triggered then it is an indication that sensor is submerged in LN2 and the liquid level is higher than desired 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER P ENTER f The display will read Te
93. itrogen or the gas use valve on a LN2 cylinder work best The LN2 cylinder vent valve can also work but will deplete the cylinder head pressure quickly Ensure the nitrogen gas pressure does not exceed 50 PSI 3 4 bar 7 Plug in the Controller main power and connect the freezer plumbing via transfer hose to a compressed nitrogen supply or the gas use valve on a LN2 cylinder Ensure gas bypass is disabled if equipped 8 Press Start Fill and allow the freezer to fill for 30 seconds 9 Press Stop Fill 10 Press Start Fill and allow the freezer to fill for 30 seconds 11 Continue cycling fills for 30 seconds until the plumbing assembly and annular lines are clear and completely dry APON In some cases it be necessary to purge the level sensing annular line separately This be done by connecting pressurized nitrogen gas directly to the freezer annular line fitting CAUTION Ensure that the LN2 supply valve is closed and the plumbing assembly is vented before loosening the compression fittings and removing the annular line tube 1 Loosen and remove the 1 4 inch compression fittings from the purge valve and the freezer annular line fitting 2 Remove 1 4 inch copper tube and purge to clear any moisture 3 Connect nitrogen gas source directly to the freezer s 3 8 inch FPT annular line connection 112 of 133 326636H01 Rev A 4 Purge annular line with nitrogen gas maintaining a pressure b
94. l CryoExtra freezer series equipped with a Controller The Hot Gas Bypass is able to vent the warm nitrogen gas from the supply line before initiating a fill cycle This prevents warm gas from entering the freezer space The bypass system helps to maintain a stable temperature gradient inside the freezer and increases the efficiency by preventing excess LN2 evaporation while filling This feature is advantageous for any freezer setup and is especially ideal for sites where longer transfer hoses cannot be avoided The Hot Gas Bypass system consists of these components Solenoid Valve 24 VDC Temperature sensor Pt 1000 RTD Muffler Plumbing and electrical connections Freezers will complete a bypass cycle prior to filling When a fill is initiated the bypass solenoid valve will open first and begin venting nitrogen gas from the supply line through the muffler and into the atmosphere The Controller monitors and displays the temperature in the plumbing system throughout the bypass cycle As LN2 begins flowing the temperature in the plumbing system will decrease Once the bypass temperature sensor readings reach the user defined Bypass Temperature Setpoint the bypass solenoid valve will close terminating the bypass cycle The fill solenoid valves will then open and the freezer will begin filling The default Bypass Temperature Setpoint is 70 C and can be adjusted based on the freezer setup The Bypass Alarm Time Delay is the maximum allowable
95. l security passwords NOTE Security Level 4 is required to setup or change passwords 7 2 6 1 Password Entry Mode This section details how to enable disable password entry mode Disabling the password entry mode will remove all Controller password protection 1 Press SETUP Higher User Level Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being Required use A Y to changed Use the A Y keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER Press ENTER for The display will read Temperature Menus Temperature Menus 3 Press SETUP Press ENTER for Until the display reads Password Menus Password menus 4 Press ENTER The display will read Password Entry Mode Use the keys to change and press ENTER to save ENABLED Password Entry Mode 68 of 133 326636H01 Rev 7 2 6 2 Global Password Change This section describes how to change the Global Password 3 4 5 6 The Global Password has Security Level 4 clearance 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature
96. larm Mask LOTB Temp B Low Alarm Query Temp A High Alarm Mask Query LOTBS Temp B Low Alarm Status Query Set Temp B High Alarm LOTBM Set Temp B Low Alarm Mask Temp B High Alarm Query LOTBM Temp B Low Alarm Mask Query Temp B High Alarm Status Query TEMPA Current Temp A Query Set Temp B High Alarm Mask TEMPB Current Temp B Query Temp B High Alarm Mask Query TUNI Set Temp Units Set Temp A Low Alarm TUNI Temp Units Query Temp A Low Alarm Query LEVEL COMMANDS Bypass Status Query HILM Set High Level Alarm Mask Fill Time Alarm Status Query HILM High Level Alarm Mask Query Set Fill Status LEVL Current LN2 Level Query Fill Status Query LFIL Set Low Level Setpoint Auto Fill Status Query LFIL Low Level Setpoint Query Set Max Fill Time LOLA Set Low Level Alarm Max Fill Time Query LOLA Low Level Alarm Query Current Fill Duration Query LOLS Low Level Alarm Status Query Set High Level Setpoint LOLM Set Low Level Alarm Mask High Level Setpoint Query LOLM Low Level Alarm Mask Query Set High Level Alarm LUNI Set Level Units High Level Alarm Query LUNI Level Units Query High Level Alarm Status Query RATE Current Liquid Usage Query LEVEL PERCENT COMMANDS DZER DZER Define 100 Point 100 Point Query Current Percent Query Define 096 Point 096 Point Query EVENT LOG COMMANDS Clear Event Log LOGPER Set Date LOGPER Date Query TIME Last Event Query TIME Number of Events Query UNID Event Log n Query UNID 88 of 133 Set Event Log
97. larm terminal Wires can be inserted and removed from the terminals by placing a small flathead screwdriver into the slot above the contact and prying down the latch until the clamp connector opens Remove the flathead screwdriver to close the clamp connector For alarm contact test procedures refer to the Remote Alarm Tests section Below are the remote alarm contact specifications and a typical remote monitoring setup schematic If an alarm condition has been corrected but it continues to register through the global or discrete contacts remove the contacts and cycle the power on the controller This will clear the alarm if it has latched in the system EMOTE Figure 7 Controller remote alarm contacts Table 2 Controller remote alarm contact specifications Discrete Contacts Global Remote Normally Open Normally Open or Normally Closed Open Collector Dry Contact Relay Latching Latching Polarity Sensitive Non Polarity Sensitive 24 VDC at 100 mA max 230 VAC at 1 A max Remote Monitoring System COMMON TEMP AHIGH DISCRETE CONTACTS Figure 8 Typical remote alarm monitoring setup schematic Temp A High monitoring shown 30 of 133 326636H01 Rev 7 1 7 Passwords Security The Controller has a multilevel security system that can be customized to meet your security needs Four different levels of security can be assigned up to 10 passwords allowing users to control
98. less Steel sheets Any cleaning solution which does don t react with Aluminum and Stainless Steel can be used in these freezers Any cleaning solution that does not react with aluminum or stainless can be used in the sanitation process of these dewars In most cases any household detergent or mild soap solution is suitable The U S Custom Service uses a solution called EXPOR for incoming shipments from abroad This is mixed 9 parts water mixed with sodium chloride amp lactic acid As mentioned above however any household cleaning solution can be used These include bleach detergents and mild soaps Other cleaners and disinfectants that can be safely used include hydrogen peroxide chlorine water and denatured alcohol NOTE DO NOT USE ANY PETROLEUM BASED CLEANING SOLUTION It is important that the inner vessel is thoroughly rinsed with water and all cleaner residues have been removed Spraying the solution into the inner vessel is preferred although agitation of the solution inside the inner will suffice The generally accepted practice of using 1096 chlorine bleach with 9096 water solution still holds as the best method for decontamination However with some of the bovine and swine virus strains showing up today it is the conclusion of the agricultural professors at the University of Minnesota and Texas A amp M that an increased mixture of chorine bleach to 30 and 70 water will kill all known viruses except BSE This is still unknown to them a
99. linder to be inoperative If you suspect this to be the case install a pressure gauge inline between the liquid cylinder and the freezer for verification 2 Verify the amount of liquid in the supply source Most bulk tanks have some method of digital or analog volume measurement Liquid cylinders typically use a sight gauge As with the pressure gauge on liquid cylinders it is common for the sight gauge to be inoperative 3 The minimum amount of liquid necessary in the supply should be enough to completely fill the number of freezers it is supplying This amount can be determined from the LN2 inch to volume table in the Appendix 4 Initiate a fill start on at least one freezer on the network The supply system should be able to maintain appropriate pressure throughout the duration of the fill cycle 5 If the supply is determined to be inadequate have your gas supplier replenish replace the supply 106 of 133 326636H01 Rev A Plumbing Leak Check Leaky plumbing connections can create a host of problems including but not limited to e Slow fill times Nuisance Alarms High LN2 Consumption Inaccurate level readings Inaccurate liquid usage readings Leaky plumbing connections are especially common on liquid cylinder supply systems since the fittings are regularly loosened and tightened during liquid cylinder swap out 1 With the supply system at operating pressure thoroughly spray all transfer hose connections and freezer plumbing
100. line s 1 kV line s to Mains power quality should be that of a IEC 61000 4 5 line s typical commercial or hospital 2 kV line s to earth 2 kV line s to earth environment Voltage dips short interruptions and voltage variations on power supply input lines IEC 61000 4 11 596 UT gt 95 dip in UT For 0 5 cycle 4096 UT 6096 dip in UT For 5 cycles 7096 UT 30 dip in UT For 25 cycles lt 5 UT gt 95 dip in UT For 5 seconds lt 5 UT gt 95 dip in UT For 0 5 cycle 4096 UT 60 dip in UT For 5 cycles 7096 UT 30 dip in UT For 25 cycles 596 UT gt 95 dip in UT For 5 seconds Mains power quality should be that of a typical commercial or hospital environment If the user of the CONTROLLER requires continued operation during power mains interruptions it is recommended that the CONTROLLER be powered by an uninterruptible power supply or battery Power frequency 50 60 Hz Magnetic field IEC 61000 4 8 3 A m 3 A m Power frequency magnetic fields should be at levels characteristic of a typical location in a typical commercial or hospital environment NOTE Ur is the AC mains voltage prior to application of the test level 118 of 133 326636H01 Rev Table 4 Guidance and manufacturer s declaration electromagnetic immunity for all Controllers See 5 2 2 2 Guidance and Manufacturer s Declaration Electromagnetic Immunit
101. lloys used is available but is not specific for the complete manufactured vessel 9 of 133 326636H01 Rev 5 Product Information This section will give an overview of CryoExtra cryogenic freezers and components ThermoFisher Scientific offers a wide range of LN2 freezers with a controller that can accommodate a variety of inventory systems designed to meet all of your cryogenic storage needs Each freezer is a hand made double walled vacuum insulated stainless steel Dewar designed to maintain temperature with minimal LN2 evaporation 5 1 Thermo Scientific CryoExtra Freezer Models Thermo Scientific CryoExtra cryogenic freezers offer specialized features and functionality Below is the list of the CryoExtra cryogenic freezer models Sno Name Model Description 1 CryoExtra 20 CE8120 110 V 230V 2 CryoExtra 40 CE8140 110 V 230V 3 CryoExtra 80 CE8180 110 V 230V 4 CryoExtra 94 CE8194 110 V 230V 5 CryoExtra 20 MDD CE8120M 110 V 230V 6 CryoExtra 40 MDD CE8140M 110 V 230V 7 CryoExtra 80 MDD CE8180M 110 V 230V 8 CryoExtra 94 MDD CE8194M 110 V 230V 10 of 133 326636H01 Rev A Figure 1 Top view of HE Freezer showing offset neck and P and R turn trays The CryoExtra High Efficiency Vapor Series freezers will maintain a vapor storage temperature of 190 C with minimal LN2 evaporation while accommodating a wide variety of inventory systems
102. lve 24 VDC R 700 8 11499812 Gas Bypass Muffler 9 11885449 Gas Bypass Muffler Deflector 12 of 133 326636H01 Rev 5 3 Controller Display The Controller front panel display is the primary user interface for the Controller There are two display options text or symbolic i OQO AOW coss m c 00 i 4 Thermo SCIENTIFIC Temrhr TemrFB uel e c oc gt 3 3 a a E A sa LDC Display 20 x 4 LCD with backlight START FILL Key Used to manually initiate a fill STOP FILL Key Used to manually terminate a fill Disables Auto Fill for 30 minutes SETUP Key Used to access Setup Menus and parameters Used to silence the audible alarm for 30 minutes Will reset the latching alarm once it has ALARM MUTE Key been corrected A Key Used to increase parameter values or to toggle YES NO or ON OFF values Key Used to decrease parameter values or to toggle YES NO or ON OFF values ESC Key Used to escape or exit a menu or menu level ENTER Key Used to select a menu or value or save a setting change 13 of 133 326636H01 Rev 5 4 Controller Stand Alone Back Panel Physical Connections The Controller physical and electrical connections are located on the bottom of the stand alone non cabinet controller and on the back panel of the cabinet controller 124 100mA MAX x am
103. ly 10 0 inches increase the offset value by at least 1 0 inch This will fool the controller into thinking that the level inside the freezer is higher than actual Reference Section 7 2 2 3 for offset adjustment procedure 4 Observe the audible visual alarm Be aware that the level alarms have a one minute This delay is intentional and is to prevent nuisance alarms If the alarm does not occur after one minute verify that the audible alarm is turned on Decrease the offset value to the original observed setting own Low Level Alarm Test Stand Alone Controller configuration Observe and record the current LN2 level Remove the vinyl tube from the hose barb on the bottom of the Controller Be careful not to damage the tube The displayed level should drop to 0 0 inches After one minute the audible alarm should sound If the alarm does not sound verify that the audible alarm is turned on Reconnect the vinyl tube If the tube is deformed at the end it may be necessary to trim off 7 of the tube to ensure a good connection Press Fill Start to purge the level sensing line After 30 seconds the level should gradually increase to actual 8 After the fill cycle is complete manually measure the level using the dipstick oor Ao N Lid Thaw Procedure 1 Open or remove lid from freezer 2 It is recommended that the freezer opening be covered with a spare lid or in another non airtight manner to prevent moistu
104. m ENABLED Temp A Low Alarm ENABLED 326636H01 Rev 7 2 1 4 Liquid Nitrogen Saturation Temperature This section shows how to adjust the LN2 saturation temperature This temperature is altitude dependent The default LN2 saturation temperature is 195 8 320 4 F 77 4 This value is accurate for altitudes ranging from sea level to 500 feet 152 m For the appropriate saturation temperature refer to Table 5 LN2 Saturation Temperatures 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A Y keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER Press ENTER for The display will read Temperature Menus Temperature Menus 3 Press ENTER P ENTER f The display will read Temp A Menu TE il Temp A Menu 4 Press SETUP Until the display reads LN2 Temperature Use the A V keys to adjust setting per Table 5 and Use A Y to adjust press ENTER to save the change ad cea LN2 Temperature Table 5 LN2 Saturation Temp vs Altitude Aude fO ONZ Saturation Temperature me X 3001 4000 915 1220 196 9 322 4 43 of 133 326636H01 Rev A 7 2 2 Liquid Level Settings This section demonstrates how to adjust the LN2 level settings 7 2 2 1 Lev
105. mber listed beside RS 485 Isolated Port in the Device Manager window Close Device Manager and click OK Select the appropriate Windows COM Port from the drop down menu Connect to Controller e For a single controller Select Single Controller and click Connect The Unit ID is automatically detected e For networked controllers Select Networked Controller enter the Unit ID of the desired controller and click Connect In order to connect to another controller on the network click Disconnect change the Unit ID and then click Connect 8 If the controller is successfully connected Port Connected and Controller Detected will be displayed in the status box NOOR NOTE Contact your CryoExtra Distributor Technical Service if you experience problems connecting to a controller 86 of 133 326636H01 Rev Downloading the Event Log 1 Connect to controller using Controller Connect 2 Select Event Download Range with Event 1 being the most recent logged event The default download range includes all events 3 Designate a filename and save location The default file name is the current date an time while the save location is the c drive 4 Click Download Event Log 5 Once the event log has finished downloading select Click to Open Downloaded Data File or open the file from its saved location Plotting the Event Log 1 Open downloaded event log with EXC
106. mperature Menus Lex Temperature Menus 3 Press ENTER Press ENTER for The display will read Temp A Menu NOTE To access Temp B Menu press SETUP T AM instead of ENTER emp enu 4 Press ENTER T A The display will read A Enabled SUP ENABLED 5 Press SETUP Until the display reads Temp A High Alarm Use the A V keys to adjust setting and press 110 0 ENTER to save the change Temp A High Alarm 6 Press SETUP The display will read Temp A Low Alarm Use the A V keys to adjust setting and press 200 0 ENTER to save the change Temp A Low Alarm Alarm masks added for Low and High Temperature alarms for both sensors A and B This allows these alarms to be enabled or disabled by the user Note these are called alarm masks because the alarms are not actually disabled They are simply no longer indicated via audio visual means The text in the menu screens uses enable and disable since that is the more conventionally understood terminology Feature is available with Firmware Version 2 03 41 of 133 7 Press SETUP The display will read Temp A High Alarm Use the A V keys to adjust setting and press ENTER to save the change 8 Press SETUP The display will read Temp A Low Alarm Use the A V keys to adjust setting and press ENTER to save the change 42 of 133 326636H01 Rev A Temp A High Alar
107. n 35 mm Auto Fill Enabled Maximum Fill Time 60 minutes Temperature Display Units C Liquid Level Display Units Inches Hot Gas Bypass MENU DISABLED Hot Gas Bypass Temp Setpoint 70 Hot Gas Bypass Alarm Time Delay 5 minutes COM 1 Type ASCII COM 2 Type Disabled Event Log Interval 240 minutes Global Password 3456 64 of 133 7 2 5 6 Restart Controller 326636H01 Rev A The Controller can be restarted in the Advanced Settings Menu Restarting the controller and cycling the power are both safe ways to reboot the controller NOTE Security Level 3 or higher is required to restart the controller from the Advanced Settings Menu 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP Until the display reads Advanced Settings 4 Press ENTER The display will read Advanced Fill Menus 5 Press SETUP Until the display reads Restart Controller 6 Press ENTER The display will read Confirm Restart Use the A V keys to change to YES 7 Press ENTER The controller will restart and the display will read Starting Please Wait Following the startup sequence the display will retu
108. n authorized CryoExtra Distributor NOTE All CryoExtra models are electrically Class 1 the AC supply requires a protective earth GND externally powered intended for continuous operation The general purpose cryogenic storage models are intended for professional use in research and the medical device versions are intended to be used in situations that directly support medical applications They are not suitable for use with flammable anesthetics This equipment has been tested and found to comply with the limits for medical devices to IEC 60601 1 2 or EN 60601 1 2 2001 or Medical Device Directive 93 42 EEC MDD The medical device versions of the CryoExtra models designated with an M at the end of the model name can be used to support a medical application as an accessory but is considered as a medical device in its own right by the medical device competent authority The M suffix models of the CryoExtra have been certified by a Notified Body to the MDD to be suitable for use in applications up to and including class lla storing of samples for eventual re introduction to the body 2 Table of contents 1 2 ERE 3 3 Satety PUPS Aldaris e aa 7 4 Certifications and BISIngs eed es 9 ES Mee C 10 5 1 Thermo Scientific CryoExtra Fre
109. nable disable the liquid usage display 1 Press SETUP Controller will prompt for a password A flashing cursor get User eves on the entry screen will make it clear which digit is being Required use A Y to changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER P ENTER f The display will read Temperature Menus em Re Temperature Menus 3 Press SETUP Press ENTER for Until the display reads Display and Output Display and Output 4 Press ENTER 1 T t Unit The display will read Temperature Units MIL 5 Press SETUP Display liquid Until the display reads Display liquid usage Use the SPEI keys to adjust and press ENTER to save ENABLED 6 Press SETUP m Liquid U Al Until the display reads Liquid Usage Alarm Use the keys to adjust and press ENTER to save ENABLED DISPLAY AND OUTPUT Liquid Usage Alarm can now be Enabled or Disabled on software versions starting with 2 03 When this alarm is disabled there will be no visual or audible indication at the controller but the event code for the alarm will continue to be recorded in the internal data log Since this alarm is the early warning to potential vacuum failure it is recommended that it not be disabled With software version 2 03 the liquid usage warning will no longer be indic
110. nd are not willing to offer a solution To perform this sanitizing procedure cover all inner surfaces with the solution let stand for 30 minutes and remove Rinse the decontaminated surfaces with clean water and remove rinse water Allow to dry before putting into service Note Units can be used immediately after rinsing but may take longer to recharge to 100 capacity 133 of 133
111. ng section NOTE Security Level 2 or higher is required to adjust the Printer settings 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER M Press ENTER for The display will read Temperature Menus Temperature Menus 3 Press SETUP P ENTER f Until the display reads Display and Output ress or Display and Output 4 Press ENTER Temperature Units The display will read Temperature Units p C 5 Press SETUP P ENTER f Until the display reads Advanced Display and Output bis ud Advanced Display 6 Press ENTER Al B The display will read Alarm Buzzer ewer ENABLED 7 Press SETUP Press ENTER for Until the display reads Printer Menu Printer menus 8 Press ENTER The display will read Print Interval Use the keys to adjust and press ENTER to save 30 min Print Interval 9 Press SETUP The display will read Print Header Press ENTER to print a new header Press ENTER to Print Header 10 Press SETUP The display will read Print Event Press ENTER to print a new event Press ENTER to Print Event 54
112. ntry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER Press ENTER for The display will read Temperature Menus Temperature Menus 3 Press SETUP P ENTER f Until the display reads Advanced Settings 1955 or Advanced Settings 4 Press ENTER P ENTER f The display will read Advanced Fill Menus ER or Advanced Fill menus 5 Press SETUP Press ENTER for Until the display reads MODBUS Menu MODBUS menu 6 Press ENTER MODBUS ID The display will read MODBUS ID Use the keys to adjust and press ENTER to save 200 61 of 133 7 2 5 4 One Fill All Fill OFAF 326636H01 Rev A This section describes how to adjust the various OFAF settings For more information and instructions on how to setup an OFAF network refer to the OFAF Network Setup section of this manual NOTE Security Level 3 or higher is required to adjust the OFAF settings 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP Until the
113. oller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A Y keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press SETUP Until the display reads Advanced Settings 4 Press ENTER The display will read Advanced Fill Menus 5 Press SETUP Until the display reads Update Firmware 6 Press ENTER The display will read Confirm Update Use the A keys to change to YES 7 Press ENTER The display will read Connect COM 1 to a PC and cycle controller power After cycling controller power the updater program should begin loading the new firmware 8 During the firmware update the display will read Communications Loss Check Controller This is normal Upon completion the startup sequence should begin within several minutes 67 of 133 326636H01 Rev A Higher User Level Required use A to Press ENTER for Temperature Menus Press ENTER for Advanced Settings Press ENTER for Advanced Fill menus Press ENTER to Update Firmware Confirm Update NO Connect COM 1 to a PC and Communications Loss Check Controller 326636H01 Rev A 7 2 6 Password Security Setup This section details how to enable disable password entry mode as well as how to change and setup multileve
114. out SETUP for next Calibeation aborted Please wait while the sensor zeros 60 seconds 60 sec delay ESC Press ENTER to save ENTER Level Calibration Complete 101 of 133 326636H01 Rev A 8 9 Advanced Settings Menus Cam 2 Type DISABLED Use f Prees ENTER to save Up Press ENTER to save Com 2 Type PRINTER Use f adjust Press ENTER to save Cam 2 Setup 9600 N31 Use 1 adjust Press ENTER 10 save Confirm Restore Press ENTER NO Restore All Defaults Use f to adjust Press ENTER 10 save Contrm Restan NO Use f to adjust Press ENTER to save 102 of 133 Security Level 3 326636H01 Rev A Setup One Fill All 8 10 Password Menus Confirm new Password NO Use 1 to adjust Use 1 to adjust Confirm new Password NO Use 1 to adjust Confirm new Password NO Use 1 to adjust Confirm new Password NO Use 1 to adjust Confirm new Password NO Use 1 to adjust Confirm new Use 1 to adjust Confirm new Password NO Use 11 to adjust Confirm new Password NO Use 1 to adjust Password 1 level Use 1 to adjust Press ENTER for next Level 1 Password 2 level Use 1 to adjust Press ENTER for next Level 1 Password 3 level Use 1 10 adjust Press ENTER for next Level 1 Password 4 level Use 1 10 adjust Press ENTER for next Level 1 Password 5 level Use 1 10 adjust Press EN
115. ow Temp B Temperature B is reading at or below the Temp B Low Alarm setting Can be Enabled When disabled there will be no visible or audible alarm indication The alarm event or Disabled code will continue to be recorded in the internal data log High Level LN level is at or above the High Level Alarm setting Low Level LN2 level is at or below the Low Level Alarm setting Liquid Usage Liquid Usage rate doubles within a 24 hour period Warning The liquid usage warning will not be indicated on the unit either audibly or visually It will only be recorded in the internal data log Liquid Usage Liquid Usage increases by a factor of 5 within a 24 hour period Alarm When this alarm is disabled there will be no visual or audible indication at the Can be Enabled controller the event code continues to log or Disabled Fill Time Fill cycle has not completed within the Maximum Fill Time setting Auto fill is disabled until this alarm is cleared Bypass Time Hot Gas Bypass cycle has not completed within the Bypass Alarm Time Delay setting Alarm Once the subsequent fill has ended auto fill will be disabled until this alarm is cleared Temp A Temperature A is reading lower than absolute zero 273 C 460 F 0 Calibration Temp B Temperature B is reading lower than absolute zero 273 C 460 F 0 Calibration Bypass Bypass sensor is reading lower than absolute zero 273 C 460 F 0 Calibration Low Battery Backup Batter
116. p lt mI HIGH LEVEL LOW LEVEL TEMP A HIGH e Le s 891 1 Temp Port Connection for Temp A probe 2 Temp Port Connection for Temp probe 3 Serial Number Barcode Controller serial number written below barcode 4 30 VDC Power Input Main power supply connection 5 Serial 1 Port RJ 45 connection for Serial COM 1 6 Serial 2 Port RJ 45 connection for Serial COM 2 7 Manual Fill Button Used to manually fill freezer When depressed and held the fill valves open When released the fill valves close 8 Discrete Contacts Open collector alarm terminals 9 Global Remote Dry contact alarm terminals 10 Wire Harness Connection 12 pin wire harness connection to plumbing assembly lid switch and battery backup 11 Level Connection Level signal input Clear vinyl tube connects to hose barb 14 of 133 5 4 1 Controller 12 Wiring Harness Connections 326636H01 Rev A MAIN WIRE HARNESS JOL GO a J 5 5 e 0 giao JOMO qnd oo INPUT ANNUAL i ae FILL JLILJ oy 90000 OOO DISCRETE GLOBAI CONTACTS REMOTE ID TEJ Y Ji POSITION DESCRIPTION WIRE COLOR WIRE GAUGE LENGTH 424VDC BATTERY 1 BACKUP DARK BLUE 18 50 24VDC BATTERY 2 BACKUP ORANGE 18 50 BLACK WHITE 3 LID SWITCH STRIPE 22 96 4 FILL VALVE BROWN 18 82 5 FILL VALVE PURPLE
117. polating that value out to a 24 hour period in order to obtain a per day liquid usage For example If the LN2 level to decrease from 6 0 to 5 5 inches in 12 hours then the displayed liquid usage will be 1 0 inch day See below This calculation is suspended during fills and will resume 15 minutes after a fill to allow the LN2 level in the freezer to stabilize The liquid usage calculation can be reset by restarting the controller or cycling the power For liquid usage display options refer to the Liquid Nitrogen Level Options section NOTE Several scenarios can lead to a temporarily exaggerated liquid usage If the level abruptly decreases 0 5 inches then there will be a spike in the liquid usage Having the freezer lid off for an extended period of time adding or removing samples and racks attempting to fill from an empty LN2 supply or moving the freezer all can lead to an accelerated evaporation rate Although the liquid usage is an accurate estimate of the daily evaporation rate it can be temporarily skewed by certain events however because of method used to calculate the usage it will recover as more data is acquired 26 of 133 326636H01 Rev 7 1 4 Temperature Measurement The Controller is equipped with two independent temperature measurement channels They are designed to be used with 1000 ohm platinum RTD temperature probes also referred to as Pt 1000 RTDs The electrical resistance of the very fine platinum wires in
118. r higher is required to reset to factory default settings 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER P ENTER f The display will read Temperature Menus TES S Temperature Menus 3 Press SETUP P ENTER f Until the display reads Advanced Settings 1655 or Advanced Settings 4 Press ENTER P ENTER f The display will read Advanced Fill Menus SSF or Advanced Fill menus 5 Press SETUP P ENTER t Until the display reads Restore All Defaults SP Restore All Defaults 6 Press ENTER The display will read Confirm Restore Use the A V keys to change to YES and press ENTER to save NO Confirm Restore 63 of 133 326636H01 Rev Controller Default Settings Controller Default Settings Parameter Default Setting High Temp Alarm 110 0 166 0 F 163 2 K Low Temp Alarm 200 0 327 6 F 73 4 High Level Alarm 8 0 in 205 mm High Level Setpoint 7 0 in 180 mm Low Level Setpoint 5 0 in 125 mm Low Level Alarm 4 0 in 100 mm Defined 100 8 0 in 205 mm Defined 096 4 0 in 100 mm Level Offset 41 3 i
119. ranty 109 of 133 326636H01 Rev SMC Solenoid Valve Current production SMC black Solenoid Valve Replacement CAUTION Ensure that the LN2 supply valve is closed and the plumbing assembly is vented before removing the solenoid valves 1 Remove plumbing shroud to gain access to plumbing system 2 Remove coil retaining clip by inserting a flathead screwdriver between the clip and the edge of the coil body Twist the screwdriver and the clip should slide off 3 Remove and discard the coil assembly 4 Using a crescent wrench loosen hex nut and remove the plunger housing Remove plunger housing and plunger assembly Discard these parts 5 Remove any debris that may have collected in brass valve body 6 Inspect the brass valve of the solenoid valve for nicks or damage If the sealing surface appears to be in good condition the valve body may be reused If the sealing surface is damaged the plumbing will need to be disassembled and the entire body will need to be replaced this is not common 7 Disassemble new SMC valve PN 142246115 using the above procedure 8 Install the new plunger plunger housing and coil assembly onto the old valve body 9 Assembly valve with new components in the reverse order 10 Verify that no leaks are present using leak detect solution 11 Open the LN2 supply valve and initiate a fill cycle by pressing Fill Start Allow the fill cycle to complete and verify that flow stops at the
120. rd Code for Information Interchange ASCII is a standard code used in data transmission in which 128 numerals letters symbols and special control codes are represented by a 7 bit binary number Below is a chart for these binary codes USASCII code chart Controller ASCII Command List Control Commands IDN ALMS ALMS CALTA Identification Query Input IDN Returned CONTROLLER Software ver Comments Used to query the controller ID Command must include preface Returns controller model and firmware version Set Alarm Status Input ALMS 0 or ALMS 1 Returned No response Comments Used to set the audible alarm status where 0 Off and 1 On Has same function as the Alarm Mute key Alarm Status Query Input ALMS Returned 0 or 1 Comments Used to query the current audible alarm status where 0 Off and 1 On Calibrate Temp A in LN2 Single Point Input CALTA Returned No response Comments Used to perform a single point calibration of Temp A Temp A sensor must be submerged in LN2 See the Temperature Calibration section of this manual 124 of 133 CALTB CALVL CALVL CODE HITSTA HITSTB INITEE LNSATP 326636H01 Rev A Calibrate Temp B in LN2 Single Point Input CALTB Returned No response Comments Used to perform a single point calibration of Temp B Temp B sensor must be submerged in LN2 See the Temperature Calibration Section of this manual
121. re from entering the storage space and to minimize the top box temperature change while the lid is open 3 Allow lid to sit at room temperature for approximately 30 minutes 4 Once thawed thoroughly dry lid cork and liner 5 Inspect lid for damage and replace parts if necessary 108 of 133 326636H01 Rev Folding Step Inspection CryoExtra freezers equipped with folding steps assemblies should be inspected for integrity at least every 6 months Verify that hinges and free of cracks and all connections are secure Check that the anti slip strips on the steps are in good condition and replace if necessary PN 4810179 Ensure the step locking strap is able to securely hold the steps in their folded position If the pivot bolts continuously loosen apply thread locker PN 11087674 and retighten Inline Filter Replacement AN CAUTION Ensure that the LN2 supply valve is closed and the plumbing assembly is vented before removing the inline filter 1 Close the LN2 supply valve and disconnect the LN2 transfer hose from the plumbing assembly fill tee Loosen and remove the fill tee and inline filter from the plumbing assembly Replace the inline filter PN 11648945 and reassemble the fill tee and filter to the plumbing assembly using new Teflon tape if needed Ensure the filter is oriented correctly so that the affixed arrow indicates the direction of LN2 flow 4 Reconnect the LN2 transfer hose open the LN2 supply valve and check
122. rn to the main monitoring screen 65 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Advanced Settings Press ENTER for Advanced Fill menus Press ENTER to Restart Controller Confirm Restart NO Starting Please Wait 326636H01 Rev 7 2 5 7 Firmware Update NOTE Controller firmware should only be updated by authorized CryoExtra Distributors or under the direction of Technical Service Improper firmware updates can render the controller inoperable The firmware or controller software can be updated in the event that a new revision is released A personal computer COM USB communications kit PN 13376947 and the current firmware updater program are required to perform an upgrade Contact your authorized CryoExtra Distributor or Technical Service for more information 1 Connect the Controller COM USB kit to serial port 1 on the Controller and a USB port on a computer 2 Start the current firmware updater program 3 When prompted select Update Firmware in the Controller Advanced Settings Menu and cycle power to the controller 4 The updater program should begin loading the new firmware 5 Once complete the controller may take several minutes to reboot It is normal for an occasional beep to sound during this rebooting period 66 of 133 NOTE Security Level 3 or higher is required to update the firmware 1 Press SETUP Contr
123. s SETUP fornest menu 97 of 133 326636H01 Rev A Press ENTER for Hot Gas Bpass manus opress SETUP tor nextmenu 8 5 Hot Gas Bypass Sensor Calibration Menus Press ENTER for Bypass Probe Calibration or press SETUP for next menu ENTER Calibration Type SINGLE POINT Use to adust Press ENTER for nex Temp to stabilize then press ENTER Bypass Temp 195 8 C Remove Hypass Probe from LN2 and press ENTER Wait while penhe wams mom temperature 180 sec time out Wait far Bypass Temp to stabilize then press ENTER Bypass Temp 0 0 C ENTER Bypass Probe two point calibration complete 98 of 133 Bypass Calibration Place Bypass Probe Wait for Bypass Temp to stabilize then press ENTER Bypass Temp 195 c ENTER Bypass Probe single point calibration complete 326636H01 Rev A 326636H01 Rev 8 6 Display and Output Menus Display liquid usage ENABLED Use 1 to adjust and Output or press SETUP far next menu ENTER Press ENTER to save Security Level 2 Use 1 to adjust 99 of 133 326636H01 Rev 8 7 Liquid Level Menus Press ENTER for Liquid Level menus Use to adjust Press ENTER to save Use f ta adjust Press ENTER to save Level Calibration Press ENTER to Start calibration ar SETUP for next 100 of 133 8 8 Liquid Level Calibration Menus Level Calibration 5 sac Press ENTER to i Start calibration time
124. simplicity The Controller will initiate a fill when the Timed Fill Start is reached later that day From then on each time the Timed Fill Frequency elapses the Controller will fill the freezer to its High Level Setpoint The Timed Fill Frequency has a range of 1 to 28 days and the Timed Fill Start can be set to any hour and minutes except midnight If midnight is selected controllers with Ver 2 03 or higher will automatically change the hour and minutes to 01 00 hours NOTE Security Level 3 or higher is required to setup or change Timed Filling 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER Press ENTER for The display will read Temperature Menus Temperature Menus 3 Press SETUP WS Press ENTER for Until the display reads Advanced Settings Advanced Settings 55 of 133 4 Press ENTER The display will read Advanced Fill Menus 5 Press ENTER The display will read Timed Fill Use the A Y keys to adjust and press ENTER to save 6 Press SETUP The display will read Timed Fill Frequency Use the keys to adjust and press ENTER to save It can be set from 1 to 28 days
125. stallation NOTE Do not connect the battery backup to the main wire harness until the power supply has been verified to power up the controller e Plug in the power supply to an appropriate wall outlet with the proper AC voltage Avoid wall outlets that are connected to emergency generator power if possible Although an uninterruptible power supply UPS is ideal to ensure continuous power a surge protector or power conditioner is recommended e Plug the power supply into the Controller 30 VDC power input The Controller display should illuminate and begin the startup sequence WARNING A A To avoid risk of electrical shock this equipment must only be connected to a properly grounded power source or outlet e Following the startup sequence the Controller may start to alarm This is normal e Press Alarm Mute to silence the audible buzzer for 30 minutes For installation and startup purposes the alarm buzzer can be disabled however be sure to enable it when installation is complete For instructions see the Alarm Buzzer page in Section 7 of this manual e Connect the battery backup to the main wiring harness While running on outlet power the Controller supplies a steady 27 VDC trickle charge to the battery backup The battery backup may need to be charged for several hours before it is able to power the Controller 20 of 133 326636H01 Rev A Remove a caplug from the fill tee on the plumbing assembly in order to
126. sword Entry Mode 5 Press SETUP Until the display reads Change Password 1 Continue to press SETUP to access passwords 2 9 6 Press ENTER The display will read Password 1 Use the A Y keys to input Password 1 and press ENTER to advance the cursor 7 Press ENTER The display will read Password 1 Level Use the A V keys to select the appropriate Security Level 8 Press ENTER The display will read Confirm new Password Use the keys to change to YES and press ENTER to save 70 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Password menus Password Entry Mode ENABLED Press ENTER to Change Password 1 Password 1 Use A Y to adjust Password 1 Use A Y to adjust Confirm new Password 326636H01 Rev 7 3 Calibration Procedures 7 3 1 Temperature Sensor Calibration This section describes how to calibrate the Controller temperature sensors There are two calibration procedures single point and two point calibration For single point calibration the reference point is LN2 For two point calibration the reference points are LN2 and ice water Unless regulations require a two point calibration the single point calibration procedure is recommended The benefit of two point calibration is more accurate temperature measurement in near room temperature environments The dr
127. t tnnt 96 8 4 MODUS ueteri patet m otia 97 8 5 Hot Gas Bypass Sensor Calibration Menus esee teens 98 8 6 Display and Output Menus oerte tae ttr ee sr 99 8 7 LIQUIG EGVEl MENUS xs societe ren bates e Dae S iuda tiet 100 8 8 Liquid Level Calibration MORBUS oot 101 8 9 Advanced Settings Menus ente tetra dehet 102 8 10 Password elis ch vet e intu 103 8 11 Controller Display ern trt rn dee 104 9 Preventative Maintenance s oeste err 105 9 1 Preventative Maintenance Schedule eese 105 9 2 Preventative Maintenance Procedures 106 9 3 Replacement Parts and Accessories 114 10 Troubleshooting Quick 115 11 Compliance Tables rocas 117 2 matices deed obeunt d quoted dedu ds qup pud eam ad LEE cet 121 12 1 Reference e I ta e erra eet e Re Rep inui Deva un VENERE RR audes 121 12 2 Table 10 LN2 Volume per Inch of Liquid in CryoExtra Freezers 122 12 3 Controller ASCII Interface teri tuus 124 13 Decontaminat
128. tand at room temperature for five minutes prior to beginning procedure 1 Press SETUP Controller will prompt for a password Use the a keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position 2 Press ENTER The display will read Temperature Menus 3 Press ENTER The display will read Temp A Menu Press SETUP for Temp B 4 Press ENTER The display will read Temp 5 Press SETUP Until the display reads Temp A Calibration 6 Press ENTER The display will read Calibration Type Use the A V keys to adjust to Two Point 7 Press ENTER The display will read Temp A Calibration Completely submerge the sensing end of Temp probe A in LN2 8 Press ENTER The display will read Wait for Temp A to stabilize Wait for the displayed Temp A reading to stabilize while the probe is submerged in LN2 9 Press ENTER The display will read Remove Probe A from LN2 Remove the temperature sensor from the LN2 73 of 133 Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Temp A menu Temp ENABLED Press ENTER to Temp A Calibration Calibration Type TWO POINT Temp A Calibration Place Probe A in Wait for Temp A to stabilize then press Remove Prove A from LN2 and press 10 Press ENTER The display
129. ting a Low Level Alarm will result This audible visual alarm will cause the Low Level discrete contact and the global remote to switch to alarm state NOTE Certain events can temporarily disable Auto Fill Control Pressing Stop Fill will disable Auto Fill Control for 30 minutes In the event of a Hot Gas Bypass Alarm or a Fill Time Alarm Auto Fill Control will be disabled until the respective alarm is cleared by pressing Alarm Mute or restarting the controller Overfill Protection The overfill protection will prevent an automatic fill if the LN2 level reading is 0 or if the level reading suddenly drops to 0 This prevents an overfill scenario in any situation where the Controller loses its ability to measure the LN2 level High Level Alarm High Level Setpoint Low Level Setpoint Low Level Alarm Figure 5 Example of automatic level control parameters Note Pressing Stop Fill will temporarily terminate Controller s automatic fill function for 30 Minutes 25 of 133 326636H01 Rev 7 1 3 Liquid Nitrogen Usage The Controller is able to calculate and display an estimated rate of LN2 evaporation inside a freezer This liquid usage is displayed in inches or millimeters per day It takes time for the real time data to accumulate in order for the liquid usage rate to be calculated The liquid usage is calculated by measuring the time it takes the LN2 level in a freezer to decrease 0 5 inches 12 7 mm and then extra
130. tion at the liquid cylinder Open the liquid supply valve on the liquid cylinder Inspect plumbing for audible and visual leaks Request repair if necessary 0 Manually initiate a fill to verify proper operation o 00N Recommended First Aid Every site that stores and uses LN2 should have an appropriate Material Safety Data Sheet MSDS present The MSDS may be obtained from the manufacturer distributor The MSDS will specify the symptoms of overexposure and first aid to be used Here is a typical summary If symptoms of asphyxia such as headache drowsiness dizziness excitation excess salivation vomiting or unconsciousness are observed remove to fresh air If breathing has stopped give artificial respiration CALL A PHSYICIAN IMMEDIATELY If breathing is difficult supplemental oxygen maybe required If exposure to cryogenic liquids or cold vapor occurs restore tissue to normal body temperature 37 C as rapidly as possible and then protect the injured tissue from further damage and infection Rapid warming of the affected areas is best achieved by bathing it in warm water The temperature of the water used should not exceed 40 C Under no circumstances should the frozen part be rubbed either before or after warming If the eyes are involved flush them thoroughly with warm water for at least 15 minutes In case of massive exposure remove clothing while showering with warm water The patient should not drink alcohol or smoke
131. troller is running on its primary power source it continuously provides a 27 volt trickle charge to keep the Battery Backup fully charged A Low Battery Alarm will trigger if the Controller is running on Battery Backup and the voltage falls below 21 volts The Controller will begin losing select functionality when its power source falls below 18 volts Non essential circuits are disabled first to conserve power The battery status can be viewed in the Add on Menus With a Battery Backup connected while the controller is running on main power the battery status screen will read On AC Power While running on Battery Backup the battery status screen will display on battery backup 26VDC 80956 10095 With no Battery Backup connected the battery status screen will display On AC Power This feature is available with Firmware Version 2 02 or higher The Battery Backup system consists of these components e Two 12 VDC lead acid batteries wired in series e Inline fuse 4A 250V F e Battery housing e Electrical connections NOTE The amount of time that a freezer will operate on power from the Battery Backup will vary depending on the fill status the fill intervals and the size of the freezer 37 of 133 326636H01 Rev A 7 2 Adjusting Settings and Options Password Entry This section describes how to access the Controller menus and adjust the various settings and options With password entry enabled the controller will prompt for a password anytim
132. us where 0 not filling and 1 filling Auto Fill Status Query Input FILLM Returned 0 or 1 Comments Used to query the Auto Fill Status where 0 disabled and 1 enabled Set Max Fill Time Input FILT XXX 128 of 133 FILT FILTIM HFIL HFIL HILA HILA HILS HILM 326636H01 Rev A Returned No response Comments Used to set the maximum fill time in minutes from 30 to 240 minutes Max Fill Time Query Input FILT Returned XXX Comments Used to query the maximum fill time Value returned in minutes Fill Duration Query Input FILTIM Returned XXX Comments Used to query the duration of the current fill Value returned in seconds Set High Level Setpoint Input HFIL XXX X Returned No response Comments Used to set the High Level Setpoint Input value uses the current level units either inches or millimeters There must be at least a 0 5 inch interval between each level setting and alarm High Level Setpoint Query Input HFIL Returned XXX X Comments Used to query the High Level Setpoint Value returned in either inches or millimeters depending on the current level units There must be at least a 0 5 inch interval between each level setting and alarm Set High Level Alarm Input HILA XXX X Returned No response Comments Used to set the High Level Alarm value Input value uses the current level units either inches or millimeters There must be at least a 0 5 inch interv
133. ve been calibrated at the factory The hot gas bypass sensor should only be calibrated if faulty readings are suspected bypass sensor has been replaced or as a part of a preventative maintenance schedule For an accurate calibration the LN2 Saturation Temperature Section 7 2 1 4 needs to be correctly set based on the altitude of the freezer location Both the single and two point calibration procedures require a small volume of LN2 enough to completely submerge the bypass sensor The two point calibration also requires an ice water bath Proper ice water bath preparation is imperative to ensure accuracy It is best to add filtered water to a Styrofoam cup containing crushed ice Allow the solution to stand at room temperature for five minutes prior to beginning the calibration procedure NOTE Security Level 2 or higher is required to calibrate the bypass sensor 79 of 133 326636H01 Rev Hot Gas Bypass Sensor Removal CAUTION Removing the hot gas bypass sensor while a LN2 supply is connected to the freezer will cause the user to be exposed to LN2 Before beginning procedure shut off and disconnect all LN2 supply sources Always wear protective gloves and face shield when handling LN2 Refer to the Safety section of this manual 1 Ensure all LN2 supply sources are shut off and disconnected 2 Remove plumbing shroud or rear panel to access the plumbing assembly 3 Locate the hot gas bypass sensor on the plumbing assembly 4
134. who has the ability to change specific settings as well as to what extent these settings may be altered For example in a tissue bank or repository that employs many technicians the facility manager may want to restrict the technicians access to allow them to view alarm settings but not have the ability to adjust the settings In this situation the technicians would be assigned a password with low level security privileges Conversely if the shift manager wants to have complete access to all menus and settings then he she would be assigned a password with high level security privileges Password entry mode can be disabled in the Password Menu For more information on passwords and security refer to the Passwords Security Setup section Password entry mode can be disabled in the Password Menu For more information on passwords and security refer to the Passwords Security Setup section Table 3 Password security levels and descriptions X denotes access to adjust setting Not Password Feature Protected Level 1 Level2 Level 3 Level 4 Start Fill X Stop Fill X Alarm Mute X Quick Reference Settings X Temp and Level Display Units X X X X Display Liquid Usage X X X X Enable Disable Temp Sensors X X X Temp Alarm Settings X X X High Temp Alarm Test X X X Lid Switch Settings X X X Hot Gas Bypass Settings X X X Enable Disable Alarm Buzzer X X X Level
135. will read Calibration Type Use the A V keys to set as TWO POINT 9 Press ENTER The display will read Bypass Calibration Submerge the sensing end of the bypass probe in LN2 10 Press ENTER The display will read Wait for Bypass Temp to stabilize Wait for the temp reading to stabilize while the probe is submerged in LN2 11 Press ENTER The display will read Remove Probe A from LN2 Remove the bypass sensor from the LN2 12 Press ENTER The display will read Wait while probe warms to room temperature Wait while the bypass sensor warms to room temperature and the controller counts down 13 Wait 180 seconds After the controller counts down the display will read Place Probe A in ice water Completely submerge the sensing end of the probe in the ice water bath 14 Press ENTER The display will read Wait for Temp A to stabilize Wait for the displayed Temp A reading to stabilize while the probe is submerged in the ice bath 82 of 133 326636H01 Rev A Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Add on menus Battery Status On AC Power Press ENTER to Hot Gas Bypass menus Hot Gas Bypass ENABLED Press ENTER for Bypass Probe Calibration Type TWO POINT Bypass Calibration Place Bypass Probe Wait for Bypass Temp to stabilize Remove Bypass Probe from LN2 and press Wait while probe
136. x This is recommended as it will be the warmest temperature experience by samples being stored in the freezer space e Connect the temperature probes to the Controller temperature ports using the twist lock connectors e For CryoExtra models once the temperature probes are in the desired position apply a small amount of the silicone sealant included with the freezer surrounding the temperature probes where they enter the sensor tubes This will help maintain their position and help keep moisture from entering the freezer storage space Proceed to the next step e Connect the clear vinyl tubing to the Controller Level Input hose barb and connect the other end of the tubing to the 3 way purge valve e Ensure all of the plumbing assembly connections to the Controller wire harness are secure 19 of 133 326636H01 Rev NOTE Do not connect the battery backup if equipped to the wire harness until later in the installation procedure e Connect the 12 pin wire harness to the Controller wire harness connection e f the freezer is equipped with battery backup measure the voltage at the end connector for approximately 24VDC to 27VDC if no voltage is present the included battery fuse must be installed before connecting the battery to the main wire harness Open the battery enclosure and unscrew the fuse harness Install the fuse close the fuse harness and the battery enclosure This is done at the factory but should be verified at in
137. xtra Checklist Signed by shipping inspector 18 of 133 326636H01 Rev Installation NOTE Do not apply power to the controller or connect an LN2 supply until later in this procedure to avoid injury or damaging the equipment Following the careful uncrating and unpacking of the freezer install using these basic instructions NOTE Only install the freezer on a level surface Never fill freezer and move to another location Always fill the freezer where it is to be installed used e Locate the temperature sensor tube assembly that will house the two temperature probes A three tube temperature sensor assembly will be installed in the center of the top of the freezer The two smaller tubes are designed to house the included temperature probes The third larger tube is designed to accommodate a third party temperature probe A silicone plug will be installed in this third tube If the third tube is going to be used simply remove the silicone plug 0 21 inches e Insert the two temperature probes into the sensor tubes and position the sensors at the preferred height in the freezer space Selecting probe A and B as well as the sensor placement is adjustable and completely up to the user ThermoFisher Scientific does recommend placing sensor Temp A at the top box level This refers to the level in the freezer space where the highest sample is being stored If storing vials in boxes then this would be at the level with the top bo
138. y The CONTROLLER is intended for use in the electromagnetic environment specified below The customer or the user of the CONTROLLER should assure that it is used in such an environment Immunity Test IEC 60601 Test Level Compliance Level Electromagnetic Environment guidance Portable and mobile RF communications equipment should be used no closer to any part of the CONTROLLER including cables than the recommended separation distance calculated from the equation applicable to the frequency of the transmitter Recommended separation distance d 1 2NP Conducted RF 3 Vrms 3 Vrms IEC 61000 4 6 150 kHz to 80 MHz d 1 2VP d 2 3NP Radiated RF 3 V m 3 V m IEC 61000 4 3 80 MHz to where P is the maximum output power 2 5 GHz rating of the transmitter in watts W according to the transmitter manufacturer and d is the recommended separation distance in meters m Field strengths from fixed RF transmitter as determined by an electromagnetic site survey should be less than the compliance level in each frequency range Interference may occur in the vicinity of equipment marked with the following symbol NOTE 1 At 80 MHz and 800 MHz the higher frequency range applies NOTE 2 These guidelines may not apply in all situations Electromagnetic propagation is affected by absorption and reflection from structures objects and people Field strengths from fixed transmitters such a base stations
139. y status will be displayed 5 Press SETUP Until the display reads Lid Switch Menus 6 Press ENTER The display will read Lid Switch Installed Use the keys to adjust and press ENTER to save 7 Press SETUP The display will read Lid Open Fog Clear Use the keys to adjust and press ENTER to save 8 Press SETUP The display will read Lid Open Alarm Time Use the keys to adjust the value and press ENTER to save 49 of 133 326636H01 Rev A Higher User Level Required use A V to Press ENTER for Temperature Menus Press ENTER for Add on menus Battery Status On AC Power Press ENTER for Lid Switch menus Lid Switch Installed NO Lid Open Fog Clear DISABLED Lid Open Alarm Time 1 min 326636H01 Rev 7 2 4 Display and Output Settings This section demonstrates how to adjust the Display and Output Settings 7 2 4 1 Temp and Level Display Units NOTE Security Level 1 or higher is required to change the display units 1 Press SETUP Controller will prompt for a password A flashing cursor on the entry screen will make it clear which digit is being changed Use the A keys to scroll to the appropriate number Press ENTER to advance the cursor to the next position Higher User Level Required use A V to 2 Press ENTER P ENTER f The display will read Temperature Menus Dons T
140. y voltage has dropped below 21 VDC Power Failure Controller main power has been disconnected and it has been running on battery 28 of 133 326636H01 Rev Can be Enabled or Disabled power for 30 minutes Lid Open Freezer lid has been open longer than Lid Open Alarm Time setting Communication Loss Controller has lost communication with the LCD display If any alarms occur contact your authorized CryoExtra Distributor or Technical Service 29 of 133 326636H01 Rev 7 1 6 Remote Alarm Monitoring The Controller is equipped with a global remote alarm relay and four specific discrete dry contacts that allow remote monitoring of alarm conditions Once activated these latching contacts will retain their alarm state until the alarm condition is corrected and cleared Although CryoExtra does not provide remote monitoring or programmable logic controller PLC systems these devices can be easily connected to the Controller For example it is possible to setup a Controller so that if a Low Level Alarm is triggered the remote monitoring system will automatically send an email or place a call to alert the necessary individuals of the freezer s status For proper discrete contact function ensure the negative low voltage terminal of the remote monitoring system is connected to the COMMON discrete contact terminal and the positive high voltage terminal is connected to the respective discrete a

Operating and Maintenance Manual 322375H01

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