950 NORTH HAGUE AVENUE TEL:(614)
Contents
1. COM amp LPT Bese md Communications Port COMI a F ECP Printer Port LPT1 vane a MetMos PEI Serial Pork COM Bevo coon NetMos PCI Serial Pork COME H Processors H Sound video and game controllers Preses F MetMos Unusable Parallel Port LPTS The name of your USB to Serial adapter will be in the list under the Ports section along with the port number currently assigned to it It s most convenient to set the port for your adapter to the lowest possible port that s not already in use You can change the port number for the serial port by right clicking it on the list and selecting Properties A tabbed property window will appear like the one below Select Port Settings from the list of tabs NetMos PCI Serial Port COM Properties General Port Settings Driver Details Resources Bits per second Emi Data bits je H Parity Nowe Stop bits hooo H Flow control Nore ss Advanced Restore Defaults cancel User s Guide for the NIBP 8 from Columbus Instruments 27 Leave all of the settings alone but click the Advanced button to bring up the following screen Advanced Settings for COM x Use FIFO buffers requires 16550 compatible UART OF Select lower settings to corect connection problems Cancel Select higher settings for faster performance Defaults Recerve Buffer Low 1 a High 14 14 Transmit Butter Low 1 i J H2. Non leakage sensor cuff problems If you did not see any bubbles from the previous test but you are not getting acceptable heart beat readings and then the sensor portion of the NIBP Monitor must be inspected Connection Problems Inspect the tubing and connections from the back of the NIBP monitor to the cuffs Make sure there are no kinks or knots in either of the lines Only allow the tubes to contact the device where they are connected Mechanical vibrations from the air pump from the table or not to place the unit on a flat surface can distort sensor waveforms Functional Equipment Test If you have proper cuff pressure and connections without leaks do a signal test Run several test scans While the scan is running rub the sensor cuff between your thumb and index finger This generates signals that are strong enough to be displayed on the graph yellow line The presence of signals indicates your NIBP Monitor and the sensor assembly are in working order Noisy Signal Readings If you are getting inconsistent readings and or the pulse graph yellow line has noise that is inconsistent with an animal heartbeat the animal is probably moving or the tubing 1s receiving noise from the environment The animal may get restless from being confined for a long time overheated loud noise or a lot of activity in the room Reduce the heat and or put a cover over animal s head The animal can also become agitated if the cuff pressure is too h
3. you will see noise in the pulse signal graph To test the tail warming system set the warming time in the setup menu to 3 minutes then click the Warmer button in the NIBP software s tool bar The tail warmer will blow warm air within several seconds The blinking red LED older style mounted near the heating cylinder on each unit indicates the heating elements are turned on The thermostatically controlled models with digital display will alternate between the dial setting and the current temperature reading If the pressure graph does not go up in the first 1 3 period of data acquisition this may indicate that your cuff system has air leakage Accordingly the system will not operate properly See the leakage part of section 6 Restrain and warm the animal s tail A restrainer is used for restraining mice to achieve a stable blood pressure reading This procedure is necessary for blood pressure measurement in conscious animals Our mouse restrainer will effectively restrain the animal but not cause aversive response This provides the possibility to monitor the blood pressure for extended times We suggest that all the parts be cleaned with soapy water then rinsed after every use To restrain the animal let its head enter the restraining cylinder first Then you can push the animal body forward until the whole body is inside the cylinder For smaller animal you can use the gray plastic removable floor provided to keep it comfortable
4. 45 200 Overall Quality ER Click for notes Baa iad Diaa 9 Rand DanS Daa Pi H H o Mm 1w Nm od Nm He o p w o fe n a m o z cy DIASTOLIC 87 mmHg AVGSYSTOLIC 111 mmHg AVGDIASTOLIC 86 mmHg APSA SSS oO oO ud WJ o oy o w Nm ud TT T 4 ee men 1 T TTT 5 i ih i sh ral ie Y i vy Occlusion Curve The larger curve on the black portion of the screen 1s a graphical representation of the pressure in the occlusion cuff over time The scale for pressure is printed up the left hand side of the graph Clicking anywhere on the curve will display the pressure at that point Sensor Waveform The smaller graph is the signal from the sensor cuff over time The high amplitude portions of the waveform at the beginning and end represent the animal s normal heart rate The signal drops to zero when the occlusion cuff occludes the artery and slowly rises back to its initial state as the pressure in the occlusion cuff is released The sensor waveform can be enlarged by using the up and down arrows on your keyboard The current state of magnification is displayed in the Notes section of the Summary Tab User s Guide for the NIBP 8 from Columbus Instruments 12 Summary Tab The Summary Tab shown here is where all relevant information about a reading is displayed The Summary Tab is broken down into three sections Summary Notes and Recommendations C
5. Position the rear stopper Item 1 and tighten the thumbscrew Item 2 Place the two cuffs Item 3 and 4 on the tail They should be as close to the tail base as possible The connecting tubing Item 5 connects the cuffs and air outlets in the rear panel of the NIBP monitor interface The correct assembly is shown User s Guide for the NIBP 8 from Columbus Instruments 21 Figure 2 Restrainer with a mouse The way to pressurize the cuffs 1s to click the Start button on your Columbus NIBP program It will start a blood pressure measurement and the cuffs will be inflated The pressure inside the sensing cuff will be kept at a user defined level to keep the cuff from sliding off the animal s tail Place the restrained mouse tail under the tail warmer For the first time measurement it usually takes about 10 minutes warming to produce adequate tail pulse signal 2 5 minutes for returning animals Set the Warming Time to 10 minutes on the setup menu Then click the Warmer button on the NIBP program tool bar to start warming The timer will count down and stop warming Measure tail blood pressure on animal After the program stops the tail warmer wait one minute to let the animal adapt To measure blood pressure just click the Start button on the NIBP program tool bar after setting the following 1 Set cuff pressures Click Setup gt Cuff Pressure Occlusion pressure 200 mm Hg Sensor pressure 45 55 mm Hg for m
6. Prostam falls tO T ner a E T a 29 ADONI E anise sav auuaeeesteaiee eect eee Error Bookmark not defined NIB D S reie T E TN E Error Bookmark not defined Columbus INSTINCTS sacnieriee eae N 29 Mouse Phy ysiolocical Dala saaicteticcnetii cbse hticeats a eae een 30 Rat Physiological DGG oirascasisesicd costed cassiendeseasacassenelesatewtagstianle Sen seutaa nina TE 31 Last Revised November 28 2008 by Ata Sayfee User s Guide for the NIBP 8 from Columbus Instruments 3 Introduction Columbus Instruments NIBP system consists of a microprocessor embedded control unit and a Windows application program The system acquires the tail artery pulse and corresponding pressure signals through a pressurized sensor cuff during the transition between inflation and deflation of an occlusion cuff The user can set inflation pressure in both the occlusion and sensor cuffs according to experimental conditions The NIBP system is capable of measuring blood pressure on up to eight animals Specifications Applicable Subjects Operating Modes Non invasive Measurements Range of Measurement Pulse Detection Method Inflation Pressure Data Acquisition Connection between NIBP control unit and computer Power Requirements Fuse Physical Dimensions Weight Rats and Mice Manual and Periodic Scan Systolic blood pressure Diastolic blood pressure Mean blood pressure Heart Rate Blood pressure Heart rate Pressurized cuff palpation
7. Settings will allow you to change the interval in which the experiment is run It takes about 26 seconds to run the experiment once In order to have enough time to heat the animal in between readings it s recommended that you use an interval of at least 45 to 60 seconds If for some reason you are not heating the animal you can use a smaller interval The automated test must be stopped manually and for beginner subjects it s recommended you test them for an hour or so at a time Animals that seem not bothered by their surroundings can be run for longer than that If at any point the animal s tail changes color or the signal strength drops abruptly despite heating stop the experiment for the sake of the subject Scanning Interval Setting x Scanning Interval seconds Range 00 00 25 to 00 16 40 Cancel Starting the Experiment After you ve set the pressures and interval at which you wish to run the experiment click Scan Start to initiate the automatic scan The NIBP Monitor will hum click and rest for a few seconds This initial noise 1s the device putting a pulse to the occlusion cuff and inflating the sensor cuff to the set pressure After the rest it will start humming continuously as it inflates the occlusion cuff After the humming the device will hiss at 1t slowly releases the air from the occlusion cuff The waveform from the sensor will look like a sine wave whose amplitude should drop to near nothing
8. Warming Time will bring up options for the Tail Warming Station The software does not control the temperature of the warmer merely the times it runs For purposes of preheating the animal you can set a timer of about 15 20 minutes Clicking on the Warmer is OFF button on the main screen will activate the warmer and display the remaining warming time on the button Usually after 20 minutes of heating you do not need to heat the animal for about 30 minutes which during this time you can take your test unless the sensor signal becomes weak As long as the animal is warm heating 1s not necessary During the experiment you ll probably want to set the warmer to Continuous Mode which will automatically maintain the temperature set on the station You ll note that while the device is taking readings from the animal the warmer will go off The combination of the occlusion cuff pinching the animal s tail and the clicking sound of the heater usually disturb the subject and upsets the data Thus the warmer turns on and runs only in between readings Cancel OF Default ee Set warming time warmer i OFF 12 minutes aN User s Guide for the NIBP 8 from Columbus Instruments 10 Scan Settings You can run the tests manually one at a time by clicking on the Start button on the Main Menu In most cases however you ll want the experiment to run itself on some kind of interval Navigating to Scan
9. the area test e Signal Strength One of the more important confidence tests is the strength test The strength test takes a sample of signal strength from different parts of the reading and uses those samples to determine if there s enough signal in the reading to make valid calculations A weak signal is also more prone to failing the other confidence tests e Noise Test The noise test looks for spikes or anomalies in the sensor waveform If the animal jerks or moves during the reading which will register as a spike on the sensor waveform Sudden extreme changes in the signal are marked as noise A reading will fail the noise test for any of three reasons o Reading is too noisy This occurs if more than ten percent of the reading has noise in it User s Guide for the NIBP 8 from Columbus Instruments 13 Notes Overall Result This is the compilation of the four major reading tests A result of Good means there 1s a good chance the data can be trusted without inspection from the user It is still highly recommended that a person check all readings because the human brain is the best pattern recognition software available A result of Fair means that the reading passed all the tests but some of them were slightly flawed A Poor reading most certainly needs to be checked by a person to see if it should be accepted A No Good reading means that the reading should be disqualified o Blood pressure region is
10. 3 Turn on the unit and the power indicator will come on 4 From the NIBP program set the warming time and click on the warmer button 5 The warmer will heat to the previous thermostat set temperature The display will read the temperature at the animal tail then every second or so it will temporarily flash the tail warmer thermostat setting the temperature that the animal will be warmed to In cases where air circulation is such that the heat generated by the heater is drawn away from the animal we recommend using the provided heater cover To change the heater thermostat temperature setting 1 Turn the thermostat knob clockwise until the display reads the desired heating temperature As long as the dial is being turned the unit will stay in the adjustment mode 2 When the desired thermostat temperature is displayed stop turning the dial After a few seconds the new thermostat temperature will flash on the display and will be locked in automatically The warmer will maintain that temperature Manual heater Stop and Idle Monitoring operation Turn knob to LOW counter clockwise and the warmer heater will automatically stop The display will read OFF After several seconds of no action controller enters IDLE state to monitor current temperature reading on the display Error Message The sensor wiring and controller are continually checked for proper operation If a malfunction is detected the c
11. 8 Channel Non Invasive Blood Pressure Monitor NIBP 8 User s Manual 0219 011M Non invasive BP Monitor Columbus Instruments 950 NORTH HAGUE AVENUE TEL 614 276 0861 COLUMBUS OHIO 43204 USA FAX 614 276 0529 www colinst com TOLL FREE 1 800 669 5011 G User s Guide for the NIBP 8 from Columbus Instruments l Table of Contents Inrodu cho neinn eee 3 SPEC A O EEE O EE T TOTEE OEE 3 The Principle of Blood Pressure Measureme n sssssscccccccccccccccccccccssssssssssssssceceecececcees 4 Blood Cir c lation ina Rat S Tailerornn T E T N Aduaiiunans 4 lalala O osaa EEE A A E E a E E REO 4 NIBE Device SCHIP ereean E Rd ausesastics dol wydeueen eae sot ance seeaasans Cotunsensesavaneactehaes 5 Tail Warming Station SCUlp arcs cries aaa as 6 PFre Experiment Setup sss csercsca ieee s aaa ssteccatancasedesauskcaiiatetauessbossaiapissedesevstcaduelesaueceiestasebiaseceeeesseess 6 ATUINGL A COUINQUION r En ENE EE aetna ae ited ee 6 Experimenl Sup dissin tava a ceataldcenties sien A EAEE A 6 Restraining the Animal cornirrcrnrsnoris ir AE EEEE 6 Cuff SOLU Or RA eiia A R A 6 SES OT CUJ op a a sh io i dn gs essa elon aes teeth eae 6 OCCIUSIOM CU ces etcdtreti cnet Gere TE O E ee teeta eee ead eee 7 Cuff Semp Jor Mite sernir a r Sa 7 Tal CHOC nee eE E O EE E TO E O 8 Executing he Experimen loreanne EE E E E 8 PCC OTTO s E oh bent Ra EE A EE T S SVALA seas casas sues A A EE E E A sade ade E EE E EA 9 Pressures CLI
12. Occlusion Cuff Sensor Cuff Resolution Sampling interval Port Format 120 240 volts AC 60 50Hz 40 watts 2Amp fast blow fuse 11 5 W X 7 5 H X 13 D 29 2cm W X 19cm H X 33cm D 12 Pounds 5 5 kg 20 290 mmHg 1 900 BPM 26 second reading time 10 sec pre reading 16 second measurement 45 300 mmHg 5 100 mmHg 12 bits 4 ms RS 232 serial port 19200 N 8 1 User s Guide for the NIBP 8 from Columbus Instruments 4 The Principle of Blood Pressure Measurement During the inflation of the occlusion cuff the tail blood will experience three distinct phases The first phase occurs once the occlusion cuff has been inflated to its user defined maximum Systolic Diastolic pressure At this point the pressure on the artery is so great that it is completely occluded and no blood passes through it The occlusion cuff then begins to release its pressure This phase 1s denoted by the flat line before the systolic point The second phase occurs once the pressure in the occlusion cuff drops below the animal s systolic blood pressure It is at this time that blood can force its way through the partially occluded artery and generate a signal for the sensor cuff This phase is represented on the graph by the ascending waveform between the systolic and diastolic points The last phase takes place when the pressure in the occlusion cuff is not enough to restrict blood flow at all and the waveform from the sign
13. Panel The window that opens will contain a list of settings one is called wil System Double click the System Icon shown here and a window like the one System below will appear Click the Hardware tab to show the screen below System Properties 7 System Restore Automatic Updates Remote General Computer Mame Hardware l Advanced Device Manager The Device Manager lists all the hardware devices installed on your computer Use the Device Manager to change the properties of any device Device Manager Driver Signing lets you make sure that installed drivers are compatible with Windows Windows Update lets you set up how Windows connects to Windows Update for drivers Driver Signing Windows Update Hardware Profiles Hardware profiles provide a way for you to set up and store different hardware configurations Hardware Profiles User s Guide for the NIBP 8 from Columbus Instruments 26 Click the Device Manager button and compare the screen that comes up to the one shown below a Device Manager File Action View Help e mE 8lgla THLOMN 3000 5 4 Computer See Disk drives B Display adapters a DYDICD ROM drives 3 IDE ATASATAPI controllers H IEEE 1394 Bus host controllers ag keyboards Mice and other pointing devices H Monitors E rs Multifunction adapters H H8 Network adapters I ak Other devices fo be RAID Controller SE rA Ports
14. VE S oonan E E ANNA 9 WPT TUS ine aiso a E T a E 9 SCAN SONOS aN E E E NETE N E E A EN 10 Sarino he TN CTIIICHL rir e e a AAA 10 NILE SOWNO ris E O N A E Il SENSO V AVC OLIN ica Cos AE dona ves ited TAEAE E AEE E ll SUMMAIY LAD oee E ahaa E A T ae ai 12 SUITING EEE EEO OE ENEO OE ENO 12 NOOS n E ET E E A E N EER 13 Recommendallon S eeii O A 13 MAKIN r EE E N E A E 13 Dalas heel cn E ne een re een eee 14 SASOS ne ne eee ee 15 D lashee OOS reckons oe te ctesh ora sca ce ea tah saad T T A A 15 Channel TADS sesonon RTA aia ACN saa OAS aA a 15 TNEM O S e T E E statues Cacaaees Ga etosuenecwancaaaeee es 16 TO EEEE AI A NE E AAEE O EEI AEE IE A A on N 16 D1 ae AAAA E EEEE RS E TEE eT ROE E EE E AA N E E E T 16 SCD i a a A a S S 17 SOO EE E E N ENAA EN EEE 17 DCU EON ELEELE NEE AEA eee ute EEE EA Meade EANA EA E AEE ETETA 18 Install Hardware and test the system integrity csssssccccccccccsssssssssccccccccccssesssccccccccccsssssees 18 Install Hardware and test the system integrity e ssssssssssssssseseeeossssesseeeeccccosseeeeecccosssseeeeeeccsssses 19 Figure 1 Tail warmer the NIBP interface and their connections cccccseccccceeeeeeeeeeeeeees 19 Fiemme 2 IRCSIVQUIICT WITH O INOUS C eia I EVEEN AANE E TNA 21 Measure tail blood pressure on QNUIMGL wcccccctis ces sedcainievss vadecusesiectanevks seicedasesasecnuves tei cieeeaes 21 User s Guide for the NIBP 8 from Columbus Instruments 2 Guide Jo Using Animal Tail WQIIMNEL sipar
15. al s tail and the sensor cuff will pick up the palpation in the tail The inflation pressure in the cuff is set by the user through the software The rate is from 5 to 100 mmHg The default sensor cuff pressure is 45 mmHg Depending on the size of the animal the pressure can be set from 35 to 45 mmHg To put the cuff together place the elastic sleeve in the cuff and pull it around one end Gently place an O ring on that end of the cuff in the User s Guide for the NIBP 8 from Columbus Instruments 7 groove cut for it Now pull the other end of the sleeve out and around the other end of the cuff so that the sleeve inside the cuff is wrinkle free Place an O ring on the other end of the cuff Make sure to use the plastic caps on both ends over the O rings to secure everything Push the caps each end of the cuff and tuck the O rings into the caps with a pen a small screwdriver or similar implement Occlusion Cuff The occlusion cuff is slightly bigger than sensor cuff The sleeve inside inflates and then slowly deflates The user through the software sets the inflation pressure in the cuff The rate is from 5 to 300 mmHg The default occlusion pressure is 200 mmHg Depending on the size of animal the pressure can be set from 190 to 200 mmHg To put the cuff together place the elastic sleeve into the cuff and pull it around one end Gently place an O ring on that end of the cuff in the groove cut for it Now pull the other end of the sleeve out and
16. al cuff resumes its regular form and amplitude Blood Circulation in a Rat s Tail The presence of continuous blood flow in the tail 1s crucial in achieving a reliable blood pressure measurement In a rat s tail there are three arteries two lateral caudal arteries and a middle caudal artery The middle caudal artery is larger than the lateral arteries In a rat with the body weight of about 350 grams its diameter is approximately 700 micrometers The tail arteries lie along the ventral side of the tail In a cross sectional view of the rat s tail many bundled tendons and a few thin muscles can be seen enclosing these arteries The tail anatomy suggests that rats do not require a large blood supply for nourishment of their tails Little evidence of tail blood flow can be seen when the environmental temperature is below 25 C As the environmental temperature increases from 25 to 28 C blood flow increases in both duration and volume Therefore in addition to nourishment another physiological function of tail blood circulation has been proposed a rat may cool its body temperature through relaxing the tail blood vessel Installation The following items are required to set up the NIBP blood pressure monitoring system a NIBP control unit b NIBP install CD Windows compatible amp test results CD opens thru NIBP software c Power cord d Serial communication cables with 9 pin male to female connectors e Occlusion and sen
17. around the other end of the cuff so that the sleeve inside the cuff is wrinkle free Place an O ring on the other end of the cuff Make sure to use the plastic caps on both ends over the O rings to secure everything Push the caps each end of the cuff and tuck the O rings into the caps with a pen a small screwdiver or similar implement The sleeve should be relaxed but not so relaxed that there is a buildup of material in the middle of the cuff Wrinkles in the sleeve are considered normal Rat Occlusion Cuff Cuff Setup for Mice The occlusion cuff is slightly bigger than sensor cuff The only difference between the two is the application of the sleeves The sensor cuff sleeve should be pulled tight inside the cuff and wrinkle free The occlusion cuff sleeve should be very relaxed in the cuff Ideally the sleeve is cut to the size of the cuff and should just slide in and then is secured to the ends with O rings The Occlusion cuff pressure for mouse is 200 mmHg and sensor cuff pressure is 45 55 mmHg User s Guide for the NIBP 8 from Columbus Instruments 8 Leakage With both cuffs setup and attached go to the NIBP software and navigate to Setup Cuff Pressure Set the occlusion cuff pressure to 200mmHg and the sensor cuff to 45mmHeg Click on Start to run a test experiment Make sure that the occlusion cuff curve has a shape similar to the one pictured below If not your cuffs are leaking Check the guide s section o
18. asuring the blood pressure through the tail cuff An optional heater cover is included with your heater Under normal usage or circumstances the heater cover will not be needed The animal can be put in a restrainer under the heater with very desirable results The heater cover is supplied to you as a convenience for use in difficult test locations If you are in a room with a strong airflow it is difficult to concentrate enough heat on the animal tail to heat the animal for proper readings In this situation put the heater cover over the animal restrainer This will concentrate the warm air on the animal tail to give you proper results When you see the animal starts moving around or its tail around or you get good results then you can remove the cover and continue to read the results from the animal You will be able to read proper results from the animal for 2 hour then you will need to put the cover back on and heat the animal again We suggest 15 20 minutes of warming time with 45 to 52 C for rats and 37 to 39 C for mice This will dilate the animal s tail blood vessels and keep this status for about one hour at 32 C room temperature During this time blood pressure can be measured Figurel Temperature controlled tail warmer User s Guide for the NIBP 8 from Columbus Instruments 24 Installation 1 Connect an A C power cord from the tail warmer to a power outlet 2 Connect the 9 pin cable from the tail warmer to the unit
19. ations of the systolic and diastolic pressures for the displayed reading The Avg Systolic and Avg Diastolic tags mark the locations of the average pressures for all displayed readings 1n the datasheet Readings marked N G are not considered for the averages Disabling or enabling readings will alter the average values and the tags will adjust accordingly SYSTOLIC 139 mmHg AYO SYSTOLIC 141 mmHg fj AYVGDIASTOLIC 106 mmHg User s Guide for the NIBP 8 from Columbus Instruments 14 Datasheet The datasheet on the right hand side of the screen contains the list of readings for this experiment Each row is an individual reading in the experiment Information about the reading is printed in columns There is a white space above the data sheet where one can enter in any miscellaneous info about this particular experiment the animal s weight medical afflictions etc e Data This column is an on off switch that determines if the particular reading should be included in any statistical calculations Those readings marked with a 7 will be considered Those marked with N G or No Good will not be considered Double clicking this cell will prompt the user to either include or ignore the reading e SBP Systolic blood pressure e DBP Diastolic blood pressure e MBP Mean blood pressure is the sum of the diastolic pressure and 45 of the difference in systolic and diastolic pressures e HR Hea
20. during the inflation of the occlusion cuff and then slowly rise to its initial amplitude during the occlusion cuff s deflation It may take several 5 15 readings before you can observe a waveform like the one below If more than 15 readings pass and you fail to observe good waveforms consult the troubleshooting section on Non leakage sensor cuff problems To stop the experiment just click the Stop button on the main form It is recommended you save the experiment to disk immediately after its conclusion to ensure the data are preserved User s Guide for the NIBP 8 from Columbus Instruments 11 NIBP Software The latest iteration of the NIBP software package has many new features and updates to better extract blood pressure data from an animal The Main Screen The main screen of the NIBP software package appears here Non Invasive Blood Pressure Monitor 2 2a f Ioj x File Edit Setup Scan Help Start Ch 1 Warmer is ON Deflate Cuffs Summary of Calculations Time 10 54 00 Systolic Blood Pressure 111 mmHg mmHg 006 Diastolic Blood Pressure 87 mmHg Mean Blood Pressure 95 mmHg Heart Rate 320 BPM SOREN TAMOS E i 114 87 96 310 10 Sen 457200 Magnification 0 f 113 0 91 313 10 46 00 45 206 Excursion Test a f 119 93 102 312 10 46 30 45 200 Area Test iii N G 10 47 00 45 200 Signal Strength 111 90 97 309 10 47 30 45 200 Noise Test a 110 88 95 309 10 48 00
21. e NIBP s Device ID View Extension Lines If checked the program will display the vertical markers for systolic and diastolic points and the envelope around the sensor waveform Maintainer einiiieiohe TTT Setup Scan Help Number of Channels Cuff Pressure Change Color warming Time Communication Port Check System Scan y View Extension Lines Start Starts the automated data acquisition from the selected NIBP channels Setting Allows the user to alter the interval between scans Channels Active Allows the user to select which channels will be scanned during the automatic data acquisition period Scan Help Start Setting Channels Active User s Guide for the NIBP 8 from Columbus Instruments Help e Topic Search Brings up the help index e About Brings up information about the NIBP software package Help Topic Search About 18 User s Guide for the NIBP 8 from Columbus Instruments 19 Install Hardware and test the system integrity System hardware includes the NIBP Monitor Item 12 and animal tail warmer Item 6 The tail warmer should be connected to the NIBP monitor via a cable Item 2 Their connections are shown below Figure 1 Tail warmer the NIBP interface and their connections Connection port between unit and serial port of PC Connection cable between unit and tail warmer Fuse holder for the tail warmer 120V 5A For 220 system use 3A fu
22. e to the file Setup exe in the root directory of your CD ROM drive Alternatively use Windows Explorer to find the file on the CD ROM and then double click the file to install For best performance the computer processor should be at least a 133 MHz Pentium NIBP Device Setup 1 Install the NIBP software package from the enclosed CD ROM 2 Connect the occlusion cuff to the Occlusion Cuff outlet port on the back of the NIBP device 1 3 Connect the sensor cuff to the Sensor Cuff outlet port on the back of the NIBP device To 4 Plug any unused channel outlet ports on the back of the NIBP Sensor Cuff device 5 Connect the device to your PC with the serial communication cable 6 Ifapplicable connect the NIBP device to the Tail Warming o C Station with a serial communication cable occlusion Plug the power cord into a proper AC line Turn on the NIBP device Run the NIBP software package 0 If prompted select the appropriate serial communication port Any one of the available COM ports can be used If the port chosen is being used by another program you will receive an error message 11 Using the menu navigate to Setup Check System Device Connected j xj Upon a successful system check you will receive the Yh Device ID IS EERS message pictured here J If you receive an error message make sure no other uff ee Columbus Instruments Device is Found in COM Port 1 Columbus Instrum
23. ents Columbus OH 43204 devices are using the communication port or select a Phone 614 276 0861 new one by navigating to Setup Communication Port 12 Once you receive the Device Connected dialog your NIBP unit is setup User s Guide for the NIBP 8 from Columbus Instruments 6 Tail Warming Station Setup 1 Ifyou have not already done so connect the Tail Warming Station to the NIBP device using a serial communication cable 2 Plug the power cord into a proper AC line 3 Ifyou are using more than one Tail Warming Station for your multi channel NIBP unit daisy chain the warmers together using serial communication cables Pre Experiment Setup There are a few things that need to be done some time before the experiment to ensure better results and cooperation from the animal Animal Acclimation The placement of animals into a new and uncomfortable environment will often elevate their blood pressure and cause them to become nervous and fidgety It is therefore advised that the animals be acclimated to the NIBP environment for a few days before any actual measurements are performed A day or two before the tests place a first time subject in an appropriately sized restrainer two or three times for about an hour at a time When the animal appears calm place them under the warmer and run it continuously at the same temperatures you ll be running the experiment 35 45 degrees Celsius Experiment Setup Afte
24. es before starting the experiment During this time the animal may become restless due to the stress of the heat User s Guide for the NIBP 8 from Columbus Instruments 9 Shaking and jerking is unacceptable during the experiment but is tolerable during the preheating stage If the jerking continues consider turning down the temperature on the heater during the experiment All animals will respond differently to the heat so don t expect one temperature to work for all Keep in mind that you do want it as warm as possible without upsetting or exhausting the animal settings Cuff Pressure Settings ume Pressure Settings Occlusion Cutt Pressure The occlusion pressure must be higher than the 230 mmHg expected systolic pressure by 80 100mmHg If your subject s average ideal systolic blood a A pressure is 120mmHg you ll want to occlude the artery somewhere approximately close to 200mmHg Going to such a high pressure gives the signal a definite occluded region The pressure set for the sensor cuff is not truly representative of the pressure being applied to the animal s tail A good deal of the pressure put into the cuff is used against the tightly applied sleeve With this in mind it is usually OK to set the sensor cuff pressure to figures higher than the animal s supposed diastolic pressure if the situation calls for it Settings between 50mmHg and 60mmHg should work well Warming Time Navigating to Setup
25. i EEE lupins dui en AOOO 23 NIU LLL 15 eee ene Or PEE RPEET SPRAY EEE Pe NEEE PRICE PRPERPE EEN Ee EE 23 ES LLL SO ee eee eee N ee ee ee ee ee ee 24 To change the heater thermostat temperature setting cccccceccccecccccccccccccceceeeeeeeaaaaes 24 Manual heater Stop and dle Monitoring Operation 0cccccccccececcccccccccccccecceeeeeennaaes 24 Error WICS SOG encase tcc us aie Sct hae ixacah M R E A O hanes atom E 24 SCV NUT SOP See REET a EC re ES ett NRT re ee eR eo ee rr ne ne ee 24 D 6 Re Ee Ie Pa TT OSE ae a ET nr OTP ea NE AOE ne eee ee nee 24 Troubles hoone serans E E seven cesdeutsuerwadeneetecussunt seven EEN 25 Serial Port problems coranica a a aaa aiea 25 Leakaze DVODICING osrore nerien r RE NE AEE NEEE EA NRN 27 Non leakage sensor cuff problems sssssssssssssseeeeeeeoesooosssssssssseeeeeeeeeeocococssssssssseseeeeeeeeeeeoessoo 28 CONNCCHIONT TODLOINE eiA EE AEAEE AE E TOT OE A O TAO 28 FUNCT ONG Equipment Test sia scale as alent Gees ee daw oaks vg Laan Sasa ed 28 NOSS Onal Kad S sisi ois ade a A 28 FDE IN OVS Ca saapsarrnc ter caticee E nese ea eee ee aa a 29 LOW Pulse SiQnd Levelei n T sa eat ee 29 Incorrect Systolic Blood Pressure Readings 0 0 0 0 cccccccccccccccc cece eneesssececeeeeeeaaeeeeeeceeeeeaas 29 Cap Unused Channel Connections ccccccccccccssscccssessseesscnccnccccccccecceeecceeeeeeeseeeeeeseeees 29 Sofware F TOD CINS eines ear EE E EATE E puesaaatiusstlaeneuaecesise ideas puesientiuseuiatins 29
26. ice 30 45 mm Hg for rats 2 Click Start button to measure the blood pressure BP Measurement in Unrestrained Male Rats 350 gm Using NIBP 700 4 SBP s00 oee HR 500 4 p 400 E 300 4 are aa ee ee 100 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Measurement Number Non Invasive Systolic Blood Pressure versus Invasive Systolic Blood Pressure 160 Non Invasive SBP Pressure mmHg invasive BP o Er a ee a D a 4 1 2 3 a S5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 User s Guide for the NIBP 8 from Columbus Instruments Figures Front 1 Rat or Mouse selector 2 Gain control 3 Power indicator 4 Power switch 5 AC Power 6 7 8 9 l Non Invasive BP Monitor HIBP amp Fuse holder Occlusion cuff connectors Sensor cuff connectors Heater connection Qasr 0 Serial port to computer crs n rrr mb leer Hea Er To Compu ler Channel User s Guide for the NIBP 8 from Columbus Instruments 23 Guide to using Animal Tail Warmer Introduction The animal tail warmer is designed for use with Columbus NIBP Non Invasive Blood Pressure Monitor Rat or mouse tail arteries are nearly closed at room temperature which means there is little continuous blood circulation in its tail However it is necessary to have in the tail a stable blood circulation for accurately me
27. igh 16 16 COME in use COM COMA fir usel The dropdown list in the lower left corner of the setting window it contains all of the serial ports on the computer Re assign the adapter to an unused serial port between 1 and 16 Leakage problems The NIBP collects data via pressurized cuffs which fit over the animal tail Because of the nature of this technique it is obvious that there 1s constant abrasion to the thin rubber sleeves on the cuffs Leakage of the sleeve is the primary problem that customers encounter This problem is exhibited in many ways The two most common are low or nonexistent pulse signal yellow line or the occlusion signal red line does not have the characteristic graph shown below z200 150 100 User s Guide for the NIBP 8 from Columbus Instruments 28 To check for this problem take the cuff holder assembly and submerge it in water with the air tubing still connected to the NIBP unit Make several test scans Air bubbles indicate you have leaks in the cuff sleeves The leak can either arise from a hole in the sleeve or a buildup or wrinkles under the cuff that prevent the O ring from forming a seal If upon inspection of the sleeve no holes can be found re apply the sleeve and be aware of wrinkles If the cuff still leaks dispose of the sleeve and use a new one Do not remove the tubing from the rear panel of the NIBP unit Disconnect the tubing from the port by the thumbscrew as seen below
28. igh Try to reduce the cuff pressure and or heat to calm the animal Low pressure and high pressure in the sensor cuff can be a problem If you are getting regular periodic noise it could be due to the animal breathing When the animal breathes its tail moves slightly in the cuff which generates noise or vibration If you observe this periodic noise try increasing the sensor cuff pressure User s Guide for the NIBP 8 from Columbus Instruments 29 Tubing Noise Place the connecting tubing where no mechanical noise such as motor or fan may create vibrations to be picked up This will reduce background noise from the sources that are near the sensor cuff or tubing Low Pulse Signal Level If the tail isn t sufficiently warmed it can cause a low signal level Increasing the warming time or warming temperature can correct this Another simple technique is to increase the sensitivity gain using the dial on the front of the NIBP device However this not only increases the size of your signal but it also increases the ability of the cuff to pick up noise If the signal from the sensor cuff is already high and the gain is turned up too much the signal can sometimes be clipped and valuable information can be lost If a number of sensitivity Gain readings are receiving clipping errors as printed in the datasheet turn the gain down on the NIBP device Low sensor signal levels can also be caused by low cuff pressure in thi
29. io 43204 2121 USA Phone 614 276 0861 Fax 614 276 0529 E Mail sales colinst com Web Page www colinst com User s Guide for the NIBP 8 from Columbus Instruments 30 Mouse Physiological Data Life Expectancy Breeding age minimum Age at puberty Estrous cycle Gestation period Weaning age Temperature rectal Respiratory rate Heart rate beats min Systolic blood pressure Diastolic blood pressure Mean blood pressure Food consumption daily Water consumption daily Urinary volume daily Body weight in grams MAP mean arterial pressure in mmHg CO cardiac output ml min CI cardiac index CO BW in kg SV stroke volume ml beat BV blood volume ml BV blood volume ml kg l 2 years 6 8 weeks 35 40 days 4 5 days 20 days 21 days 96 4 100 F range of averages Average 163 breath per min minimum 84 maximum 230 Average 580 25 per min It ranges from 328 780 120 30 90 30 100 30 4 6 Grams 6 milliliters 1 2 milliliters 25 30 Grams 92 3 12 1 4 591 80 0 018 0 002 2 3 0 09 80 4 All values can vary depending on age and sex of animal User s Guide for the NIBP 8 from Columbus Instruments 31 Rat Physiological Data Life Expectancy Breeding age minimum Age at puberty Estrous cycle Gestation period Weaning age Temperature rectal Respiratory rate breaths per minute Heart rate beats min Systolic blood pressure Diastolic blood pressure Mea
30. licking the button on the bottom of the tab will cycle through the sections Summary Summary e Systolic Blood Pressure systolic Blood Pressure 139 mmHg e Diastolic Blood Pressure Diastolic Blood Pressure 96 mmHg Mean Blood Pressure 110 mmHg e Mean Blood Pressure Heart Rate 431 BPM e Heart Rate Channel Number 1 e Channel Number Magnification 0 e Magnification The current state of Excursion Test magnification on the sensor waveform Area Test Moise Test D signal strength pmm D Overall Qualty Dmm Click for notes At the conclusion of a reading four tests are performed on the data and their results are quantified and displayed in the form of colored bars Green is Good Yellow is Fair Orange is Poor Red is No Good These qualitative results are automatically set as the comments of each reading They can be altered by the user at any point e Excursion Test Given the rate of deflation in the occlusion cuff the rate of ascension in the sensor cuff s signal should be relatively linear At the very least the rate of ascension shouldn t vary a great deal The excursion test is scaled on the linearity of the ascension linear being the best and curved being least favored e Area Test The amplitude of the sensor waveform should be relatively uniform following the diastolic point Large differences or discrepancies in the amplitude of the waveform following the diastolic point will fail
31. n Leakage Problems 200 150 100 Tail Cradle As with humans blood pressure should be read from a point on the body at the same elevation as the heart With this in mind the cuffs should be placed as close as possible at the base of the tail When running the experiment you may need to prop up the end of the restrainer so it is flushed with the tail cradle Executing the Experiment Once all of the pre experiment procedures are complete you can begin testing your animals Preheating In order to get a sufficient signal from the animal s tail there must be good blood flow through it To improve flow heat is applied to the animal but mostly to the tail to increase circulation Clicking on the Warmer is OFF button in the NIBP interface software will activate the heater at which point the heater will take a temperature reading and display that temperature Any turning of the heater s knob will change the display to the target temperature Go to Setup Menu Click on Warming Time Check The Continuous Mode and Click on OK Once that temperature has been set the station will begin to heat the tail flashing the current temperature on the display When the current temperature reaches the target temperature the heater will turn off and automatically start back up again when the sensor temperature falls below the target temperature The heater should be run in continuous mode at the experimental temperature for 15 20 minut
32. n blood pressure Food consumption daily Water consumption daily Urinary volume daily Body weight in grams MAP mean arterial pressure in mmHg CO cardiac output ml min CI cardiac index CO BW in Kg SV stroke volume ml beat BV blood volume ml BV blood volume ml kg 2 3 years approx 72 days vaginal opening on 42nd day 60 72 days 4 5 days 22 days lactating female 24 34 days 21 days 99 5 100 6 F range of averages range of averages 85 5 113 BPM minimum 63 maximum 179 range of average 350 400 BPM minimum 300 maximum 504 120 30 90 30 100 30 12 15 Grams 25 milliliters 11 15 milliliters 250 400 Grams 100 125 100 300 0 25 20 55 60 e all values can vary depending on the age and sex of the animal
33. nfidence tests e Exit Program File Edit Setup Scan Help pen Ctrl 0 Close Current Data File Save 45 Cerl 5 Export Print Figure Ctrl P Exit Program Edit e Save Datasheet as TXT Saves the summary information from the datasheet in a simple text file This is a useful option for printing e Clear Datasheet This will remove all readings from the datasheet e Delete Current Reading This option will remove the reading currently displayed Edi Setup Scan Help Save Datasheet as TXT Clear Datasheet Delete Current Reading User s Guide for the NIBP 8 from Columbus Instruments 17 Setup Number of Channels Opens the dialog for selecting the number of channels in the experiment animals Cuff Pressure Allow the user to set the pressures of the occlusion and sensor cuffs Change Color Opens the color of dialog for the plotting region of the page You can change the background foreground occlusion and sensor wave colors Systolic and diastolic markers will always be white and green respectively Warming Time Opens the dialog for setting a timer on the warmer or setting it to continuous mode Communication Port Opens the dialog for selecting the port on the computer to communicate with the NIBP device Check System Sends a signal to the NIBP device If the signal is received properly the device will send back the contact address for Columbus Instruments and th
34. ontroller will automatically switch to OFF The heating element will be deactivated and the display will show Err Safety heater stop The heating element stops automatically when a malfunction is detected or temperature exceeds upper limit Safety The heating element has an overheat breaker to prevent it from overheating The breaker may fail if a piece of dirt is sucked inside of it Therefore we suggest the user clean the tail warmer compartment routinely User s Guide for the NIBP 8 from Columbus Instruments 25 Troubleshooting The NIBP is a complex combination of computer and pneumatic equipment It cannot be stressed enough that the manual should be read before attempting to set up or to run the NIBP The time spent will be rewarded in a better understanding of the equipment This will translate into more accurate data collection and a more trouble free operation of the equipment Serial Port problems Customers whom are using computers without standard serial ports usually use a USB to Serial adapter Most normal serial ports have port numbers less than 16 but such is not always the case with adapters If you seem unable to connect to the NIBP device on any of the 16 ports available from the Communication Port menu you ll need to check what serial port your device is actually connected to and renumber it To check the port number for your adapter click Start gt Settings gt Control 4g
35. ove all readings if you want to scrap the data sheet e Delete Current Reading This option will remove the reading currently displayed not necessarily the one you clicked This is useful if there is a reading in the experiment that is useless because of animal movement or very poor signal strength Channel Tabs The tabs displayed above the data sheet are the different channels for the given experiment If the experiment was conducted on a multi channel system with multiple channels in use the readings from each channel are stored on their own data sheet under the appropriately numbered channel tab Clicking each tab will bring up the data sheet for that channel User s Guide for the NIBP 8 from Columbus Instruments 16 The Menus Many of the program s features are accessible through the menu bar across the top of the main screen File e Open Opens previous experiments in the form of DAT files e Close Current Data File Closes the currently opened data file Will prompt you to save it if it has not been previously saved e Save As Saves the currently open experiment as a DAT file e Export Exports the raw data from the current reading as a CSV coma separated value file Useful for creating graphs of the reading in an external spreadsheet program e Print Figure Prints the current occlusion curve and waveform with systolic and diastolic points marked The printout also features the results of the co
36. r the animal has been acclimated to the NIBP environment there are steps that need to be taken immediately before initiating the experiment Restraining the Animal The restrainer should be a snug fit but not too tight You want a restrainer that will prevent the animal from rotating his body and restrict its feet from kicking or moving the restrainer To put an animal in the restrainer grasp it firmly by the tail and have the head goes in forward through the restrainer so its tail is completely out the hole in the back Insert the end cap into the restrainer and move it toward the back of the animal so its nose pokes through the holes of the front of the restrainer Tighten the end cap by turning the screw on top Some animals may become nervous during the experiment if they can see you it might be necessary to cover the restrainer with a piece of dark cloth a box or anything that might obstruct its view The NIBP device needs to filter signals differently if it s using rats or mice Make sure to flip the metal switch on the front of the unit to the appropriate animal Leaving the switch on Mouse when experimenting with rats will make the resulting waveform look noisy If the switch 1s set to Rat while experimenting with mice the software will not accurately be able to discern heart rate Cuff Setup for Rat Sensor Cuff The sensor cuff is slightly smaller than occlusion cuff The sleeve inside inflates and gently grips the anim
37. rt rate in beats per minute e Time The time the reading was taken e Sen Occ The pressure settings for the sensor and occlusion cuffs For example 45 200 means the sensor cuff was set to 45mmHg and the occlusion cuff was set to 200mmHg e Comments The software automatically generates comments about the reading based on its quality but you can delete or edit these comments User s Guide for the NIBP 8 from Columbus Instruments 15 Statistics Clicking and holding the left mouse button while selecting more than one cell will give you statistics on the cells you have selected BERR 97 105 434 i 295 ERE Fi 118 374 5 i EENECLHETEEEE Column 3 SBP i ae Average 141 03 mmHg Fi Ga Standard Deviation 10 24 mmHg 5S BRR Eee yerage 39 mmHg a ly Standard Deviation 9 15 mmHg EEF oo a EH E o RORURGHE Som 5 wor Average 117 95 mmHg F 4i Standard Deviation 8 74 mmHg f 5i f a E Column amp HR Average 429 11 BPM rii Standard Deviation 12 63 BPM j2 341 s PSE 331 EH ROFE EFA o EE Li BEEIRDEIEEEIER i 291 Ee ri 146 127 369 4 c MPESA l a a 5 Datasheet Options Right clicking on the data sheet brings up the Edit menu with the following options e Save Datasheet as TXT This will save the summary information from the data sheet in a simple text file This is a useful option for printing e Clear Datasheet This will rem
38. s case P increase the cuff pressure The sensor cuff being too far from the base of the tail can also cause low signal levels Make sure both the sensor and occlusion cuffs are as close to the base of the animal s tail as you can get without applying a force on its back with the cuff Incorrect Systolic Blood Pressure Readings Overheating the animal can cause high systolic blood pressure readings The remedy for this 1s to turn off the heater and wait 1 3 minutes Then take readings to see if the systolic pressure drops as the animal cools down Unusually high systolic pressure readings can also stem from having applied the occlusion cuff sleeve too tightly If the sleeve is stretched within the cuff the device wastes pressure pushing the sleeve up against the animal s tail Make sure the sleeve is applied so it is not stretched but not so sloppily that excess material builds up inside the cuff Cap Unused Channel Connections This applies only to multiple channel NIBP Monitors For example If you have a four channel unit and are only using one the unused occlusion channels must be capped Software Problems Program fails to run The NIBP software package is written in Visual Basic 6 0 and therefore requires the VB6 Runtime files in order to run properly See the Microsoft website for information on downloading the latest VB6 Runtime files Columbus Instruments Columbus Instruments 950 North Hague Ave Columbus Oh
39. se Connection tube from the sensor cuff outlet to the sensor cuff Connection tube from the occlusion air outlet to the occlusion cuff Tail warmer Sensor cuff connector that is labeled with To Sensor Cuff Occlusion cuff connector that is labeled with To Occlusion Cuff 9 Sensor cuff 10 Occlusion cuff 11 Animal restrainer 12 NIBP monitor unit 13 Fuse holder for the control unit 120V 2A For 220 system Use 1A fuse 14 Thermostat knob 15 Cover oe a a Note After these two devices and cuffs are connected the user must seal all of the unused cuff ports in the rear panel of the monitor if the device has more than one channel The serial connection cable should be connected to your PC Plug the two device s AC power cords to your power outlet then turn on the monitor s power switch located on the front panel The green LED will light up on the front panel and you will hear valve noise coming from inside the instrument The NIBP monitor takes about 10 seconds to calibrate its amplifiers and pressure sensors User s Guide for the NIBP 8 from Columbus Instruments 20 To test the integrity of the automatic air supply and data acquisition system select channel 1 then click the Start button on the NIBP software s channel tool bar You should be presented with a typical pressure graph in upper portion of screen and a pulse signal graph on the bottom During the signal acquisition if you touch the sensor cuff
40. sor cuff per channel with occlusion cuff inserts f Sets of Tygon tubing used to connect cuffs to control unit g Animal restrainer or unrestrained cage one for each animal h Tail Warming Station required with restrainers 1 IBM compatible computer provided by user minimum requirement 133 MHz Pentium User s Guide for the NIBP 8 from Columbus Instruments 5 Please contact us if your AC voltage is not 110 120 V or different from those specified on the back of the control unit On the front panel of the NIBP control unit there are a Power switch Power indicator Rat Mouse selection switch and Gain dial On the rear panel you will find the following connectors AC power input it should be plugged into the specified voltage source Fuse holder 120Vac 2A or 220vac 1A Use fast blow type fuse 9 pin female connector for the connection of the control unit to the computer Serial port Depending on the system to 8 pairs of connectors occlusion and sensor cuffs should be connected to these ports 5 9 pin female connector form the control to the Tail Warming Station a The NIBP software is provided on a CD ROM disk It is written to operate on a Windows based computer To install the software insert the setup CD disk into the CD ROM drive Depending on the configuration of your computer the installation program starts automatically If it does not select the Windows Taskbar Start button choose Run then brows
41. too noisy This condition is met if there is a buildup of small noise in between or near the systolic and diastolic points This can also be met when the ascension from systolic to diastolic takes an unusually sharp increase It s not necessarily noise but it makes the reading unreliable Large spike s in the blood pressure region This happens when the animal jerks close to the systolic point or diastolic point When the spikes are strong enough they are marked on the waveform with an exclamation point and a red beam pointing to the interval or intervals that are too noisy While the intervals in question might not necessarily be noise they are significant sudden changes in amplitude Anything unusual about the reading is listed in the box Some notes are made because there is something about the waveform that needs to be checked by hand Others are made simply so that the user knows what was wrong with the waveform if it failed any one of the tests particularly the noise test Recommendations If the reading failed a test for preventable reasons the solutions to those problems are listed here Not all problems with a reading are fixable Sometimes it s just bad luck and a bad reading Most problems with a reading have simple procedures that can be done to prevent the problem from repeating Markers There are four markers that appear on the occlusion pressure curve The Systolic and Diastolic tags mark the loc
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