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Smartline Pump 1050 user manual

1. 2c eee eee eee eee 15 Rear view of the device with connections 0 15 Attaching the snap ferrite 0 3 5442456 4444508 4 0o0e ses Cao ks 16 Connecting the flat ribbon cable with the connector strip 17 Remote terminal strip i ss ove Stes ov eee eee tas ewer Keke bart 17 Relation between display and Events terminal strip 18 RFID designation in the pump display 04 19 Connecting the eluent line at the pump head 21 Intake manifold with four inlets 2 0 2c eee eee eee 22 Connecting the intake manifold to the pump head 22 Screwing the eluent line into the intake manifold 22 Connecting the SmartMix mixing chamber HPG system 23 Display and keypad tad eeu ches oa ted eae sete wea eed 24 Controlling the main menu vi26 o34 2 sek KORRES Os OEE Se Sas 24 Structure of the Setup Menu ss c52ocerc dc ded te dew ease hdow ss 27 Structure of the GLP menu v2 26 esky erence eave eek eee 4 ta ee 31 Setting the flowrate cus uke tae a DRESS BOER OR eR ae 33 Creating a PIOGIAIN seses eere aug Tene oe aS eR ee he SM 34 Running a program estes See Reh Ss ee BERR RR 35 Changing a program 3 42 2509 284 2e et Ghai Ges tae e eee Os 36 Deleting program lines 0 eee eee eee 37 Deleting a program ata sek pede newens ee eea heen eae eee he 38 Creating a wakeup program 0 cece ee eee ee ee eee 39 Creating a NIP ice
2. Connecting an intake manifold to the pump head in the LPG system Steps Figure 1 With the knurled head screw B screw the intake manifold into the lower inlet fit ting A of the pump head 2 Rotate the mixing unit until the inlets of the intake manifold C is at the side 3 Tighten the knurled Fig 12 Connecting the intake f BeN manifold to the pump head 4 Screw the fastening screws D of the elu ent line into the inlets of the intake mani fold 5 Use filler caps to close unused inlets Fig 13 Screwing the eluent line into the intake manifold 23 Legend A SmartMix mixing chamber B Pressure sensor C Pump head Controlling the pump Mixing chamber KNAUER recommends using an eluent mixer with all gradient sys tems The best results are obtained with SmartMix mixing cham bers which can be used both in high pressure and low pressure gradient systems Connecting a SmartMix mixing chamber gt With two screws attach the mixing chamber at the front panel of the device Fig 14 Connecting the SmartMix mixing chamber HPG system Controlling the pump Procedure Operator errors and clogged capillaries can cause pressure spikes gt To avoid damage to the pump head never allow the pump to run without liquid in the pump head and piston backflushing components Switch on and self test After the device is switched on HPLC Pump and the software ver sion appear on
3. HPLC SMB Osmometry Wissenschaftliche Geratebau Phone 49 30 809727 0 Dr Ing Herbert Knauer GmbH Telefax 49 30 8015010 Hegauer Weg 38 14163 Berlin Germany E Mail info knauer net Internet www knauer net Ih KNALIER KNAUER 2012 V7608A 0 1 10 12 Koe
4. Inspect the eluent filter of the HPLC column and change when blocked Exchange the pump head Clean the ball valves Exchange the ball valves If the pump head seals are defective solvent enters the piston backflush ing inform the technical support of KNAUER Check the following options Clean the ball valves Exchange the ball valves Purge the pump head to remove the air bubbles Always tighten the inlet screw 1 and outlet screw 1 on the pump head with a torque wrench and 15 Nm 53 Troubleshooting Problem Pump head leaks Flow rate is not correct Solution Check the following options Inspect the inlet and outlet screw fittings of the pump head Exchange the pump head If the pump head seals are defective solvent enters the piston backflush ing inform the technical support of KNAUER Check the following options Check the data for the solvent com pressibility Clean the ball valves Exchange the ball valves System messages The system messages of the display are explained They are sorted alphabetically System message Auto pump head type head data uninitialized Auto pump head type no head detected Cannot delete active program link Cannot edit pro gram from the run ning link Cannot initialize LAN Cannot operate with an empty link Solution Repeat the automatic configuration in the chromatography software Remove the pump head clean it and
5. Press horizontal arrow key to go to the editing mode of the submenu 4 Press START STOP the display switches to OFF mode 5 Enter program number then press horizontal arrow key the display switches to HOLD mode 6 Place the cursor onto HOLD and press 1 the display switches to RUN mode program is running 7 After the program has been completed the display switches to END mode Press START STOP Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog lt gt Setup Link lt gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog P01 1 000 0416 00000000 Setup Linka 000 0 100 O O O J GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO0 on1 JL2 Prog P 1 000 000 gt Setup Link lt OFF 000 00 100 0 gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO0 on1JL2 Prog P01 1 000 000 gt Setup Link lt HOLD 000 00 100 0 gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO0 on1JL2 Prog P01 1 000 000 gt Setup Link lt RUN 000 10 83 17 gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO0 on1JL2 Prog P01 1 000 000 gt Setup Link lt END 000 50 0 100 gt GLP Hold 0 Time A B C D Run 1 min Fig 21 Running a program 36 Procedur
6. Remote p45 52542 SiGe the ix teeg PEGA wae wpe Be RAK G 17 Terminal strip Events d6 2 6 s8 o Shek Salded eid bee ss ems ee 18 Entering control signals in the program 2 eee eee eee 19 Controlling the pump with chromatography software cc 2c tesa ews cee runerne Ke bw ee aw Swe oe 19 REID GECOGMINIOIN aS wits ee ah ie Lake ah gs Pinel Seat rec an Pieler tire lee ag 19 Eluent line iee sine batters Stem ince E TE E E E Bie pa a a oe tg a 20 Connecting the eluent line in the isocratic system or HPG system to the pump head 00 00000 21 Intake manifold ows Soe Na es we Se See ae ae aes aes 22 Connecting an intake manifold to the pump head in the LPG system 22 MIXING ChamMbeRs lt ssie oidin a a Ee MARAE Re eae BR ae TO Routed 23 Controlling the pump 055 cis oe ee eee bw ee ae ee Ree wee 23 Switch on and self test aces ei aiad oa ave aera kat an wie BE Cit eda Saeed 23 Display and keypad essesi erties tennint duren a by echt tiouw ved ees 24 Controlling the main menu 064 55 ee P NS eSNG Se ee SG ON ES ae 24 Selecting the Setup Menu 2 wee Reet Vek ota eee see ewes 25 Selecting the GLP menu lt 3 ts eM oer thet Beat eo Hota ewe tee eat 25 Selecting the Link menu ia 4 c08 oa bed HOS ee eK ROR eS 25 Selecting the Program Menu 2 eee cee eee ee eens 25 Controlling the Setup menu 42 24 64 S Be ee VR eee aes cs oho YG Bs 25 Structure of the Setup Menus 4 x 2044 4 4aSkeeenee ea dete eee ee
7. The output range can be set to values of max 1 2 5 and 10 V Analog input signal for controlling the flow rate e g 1V for 1 ml min in the case of the 10 ml pump head 1V for 5 ml min in the case of the 50 ml pump head Output is active for 500 ms when the pump starts Activated by a voltage of 0 V against GND Output is active until the Error condition has been eliminated A voltage of 0 V against GND stops the pump Terminal strip Events For test purposes or in some other cases it can make sense to manually enter these signals Sending control signals Events to external devices Opening and closing contacts Activating 500 ms pulses Legend A Display f Prog lt B Terminal strip A Link lt Events Fig 8 Load Flow Prog ml min 0 1MPa offO0 on1 JL2 P01 01 00 0200 01000010 Setup OFF 000 0 100 gt GLP Pressure Events Relation between display and Events terminal strip 19 Procedure Open control protocol Connecting the pump with other devices Entering control signals in the program 1 Select the program menu Select the program number 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Enter values for events in the upper status bar by using the vertical arrow keys Value Explanations 0 Off 1 On Jl Pulse No status change Controlling the pump with chromatography software The pump can be controlled individually or a
8. With dynamic mixing chamber 6 4 kg 390 x 226 x 135 mm Devices and accessories Name Pump with installed 10 ml pump head stain less steel accessories Pump with installed 50 ml pump head stain less steel accessories Pump with installed 10 ml pump head stain Order number A50353 1 A50363 A50351 1 less steel with titanium inlays accessories Pump with installed 50 ml pump head stain A50361 less steel with titanium inlays accessories 58 Delivery program Manual V7608A 1 Spare parts 10 ml pump head stainless steel available A54123 1 until 2012 10 ml pump head stainless steel available A54123 2 from 2012 10 ml pump head stainless steel with titanium A54121 1 inlays available until 2012 10 ml pump head stainless steel with titanium A54121 2 inlays available from 2012 50 ml pump head stainless steel A54133 50 ml pump head stainless steel with titanium A54131 inlays Network cable A5255 Set of connector strips A1420V12 2 x connector strip 12 pos stylus 2 x flat ribbon cable 1 5 m 10 pos A1467 Ball valve A0684 Power supply cable Power supply cable Germany M1479 Power supply cable United Kingdom UK M1277 Power supply cable USA M1279 59 Legal information Legal information Warranty Conditions The factory warranty for the device is valid for 12 months after the date of dispatch All warranty claims shall expire in the event that any unau
9. 39 Procedure Controlling the pump Creating a program with preselectable start time The pump can start a program at a preset time WAKE UP pro gram This program is preset as number 20 The pump starts the selected program at the specified time and the display switches to RUN mode program is running gt Make sure that the date and time configured in the Setup menu are correct 1 Select the Program menu Enter program number 20 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Enter the date and time for the program start 5 Enter the number of the program that should start at the preset time or select the link 6 To interrupt the current WAKE UP program enter another program number Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog Open P20 01 Setup Link lt gt GLP Hold 0 Time A B C D Run 1 min S Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog P20 Time 12 09 WAKE UP Setup Linka Date 19 01 10 P j gt GLP Hold 0 Time A B C D Run 1 min i Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog P20 Time 15 00 WAKE UP pSetup Link lt Date 19 01 10 P01 j gt GLP Hold 0 Time A B C D Run 1 min Fig 25 Creating a wakeup program 40 Navigating through the Link menu Practical tip Controlling the pump Controlling the Link menu The Link menu displays the status of active li
10. Support 022 eee eee 47 Maintenance contract 1 5 osnce esa eed eu eiich ss aoa meee Ae OE ae Oe x 47 What maintenance tasks may users perform on the device 47 Tightening the Screw fittings on s c lt vs bee eee tes beens 2 Sees e se ees 47 Loosening the screw fittings ce cc ee eee eens 48 Leaks in the capillary screw fittings 0 eee eee ee eee 48 Replacing the pump ihead s isc e2hu tegen dy Cane sae eee eee es Caeewes 48 Removing the pump head was508 444 es hee a eh dew headed whale 48 Installing the pump head o 5 lt hcscie nes oes ewekeates Reese he ees 49 Exchanging the ball valves 3 u geae4t been eens eee aie Ree eS KER 49 Removing the ball valves 0 0 eee eee eee eee teens 50 Cleaning the ball valves 3 0 s64 28 4 saaced caden Saw iteaeu deeds 50 Installing the Ball valves s25 casa sedew snd e ead sev eae e cad ad dae wed 50 Cleaning and caring for the device cee ee eee eee eee 51 Environmental protection 0 0 ce eee eee eee eee eee 51 Disposal sereta be ee eee eee Saget OE Ae Ea Re Re ER A N 51 Decontamination sess eee aana e Re en aces bee ee ee ete een 51 StOMAGQE s senn iea Ake eR oe AR RY OPE ERLE Re SOO ES 51 Ambient storage conditions for the device 000 eee 51 Troubleshooting G008 5 fone Akela ho es oes Ratha OA 52 Error liSt and solutions speen Yeas Cues nee eee Se ea ee ee ew sala oe 52 SYSCEMAINESSAQES oy sade Sete stese
11. gt 5MPa Setup Link lt OFF gt gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO0 on1JL2 Prog lt PURGE 3 000 000 25MPa Setup Link lt PURGE gt gt GLP Hold 0 Time A B C D Run 1 min Fig 30 Purging the pump in the HPG system 46 Purging the pump Piston backflushing The pump is equipped with automatic piston backflushing It increases the service life of the seals and pistons and removes contaminants from the area behind the seals The piston backflushing function automatically flushes the rear piston area of the pump head upon switch on and in continuous mode Upon switch on The rear piston area of the pump head is automatically flushed for 8 seconds In continuous mode The rear piston area of the pump head is flushed automatically every two hours for 15 seconds The flushing interval can be changed in the chromatography software Recommended The rear piston area is either flushed with water with an 80 20 washing solution mixture of water and methanol or with isopropanol depending on the application Legend A inlet from solvent bottle to flushing pump B Outlet from pump head to solvent bot tle C Inlet to pump head D Outlet from flushing pump to pump head Fig 31 Connectors for piston backflushing 47 Maintenance and care Maintenance and care Contact data for KNAUER Technical Support European hotline E
12. install it again Switch the device off and on Repeat the automatic configuration in the chromatography software Remove pump head clean it and install it again If the pump head cannot be detected automatically select the pump head manually at the device Pause active program or link After wards delete the link First pause the link then edit the data on the device display or in the chroma tography software Check the cables and connections in the local area network The link is empty First create a link Troubleshooting System message Cannot read data from FRAM Cannot read RTC Cannot start time table Cannot use non existing component Cannot write data on FRAM Error input activated Insufficient access Invalid command Invalid parameter s Invalid time in time table Invalid time table index Link is loaded Link is running Maximum pressure System stopped Minimum pressure System stopped No components are available in iso cratic mode No link available No link available Pls edit link first No time table to start Solution Switch the device off and on If the system message appears again notify KNAUER Technical Support Switch the device off and on If the system message appears again notify KNAUER Technical Support Edit the data at the device display or in the chromatography software Change the setup settings or change the gradient in the
13. notify KNAUER Technical Support Change the line index in the link Check the link number Values from 1 10 are possible Correct the entry 4 40 C 39 2 104 F below 90 humidity non condensing Dual piston pump with main and auxiliary piston 10 ml pump head 0 001 9 999 ml min 50 ml pump head 0 01 49 99 ml min 10 ml pump head A54123 1 available until 2012 40 MPa up to 10 ml min 10 ml pump head A54123 2 available from 2012 60 MPa up to 5 ml min 40 MPa up to 10 ml min 50 ml pump head 20 MPa up to 25 ml min 15 MPa up to 50 ml min 1 for intermediate flow rates with a mixture of 10 ethanol and 90 water RSD relative standard deviation 10 ml pump head lt 0 1 1 ml min 50 ml pump head lt 0 3 5 ml min Delivery program Residual pulsation Automatic piston backflushing Gradients System protection Control Programming Supply voltage range Supply frequency Active power con sumption Weight Dimensions length x width x height Delivery program lt 0 5 1 ml min Standard Isocratic HPLC pump Pump with 4 channel gradients and manager low pressure gradi ent system LPG Up to 4 pumps combined high pressure gradient system HPG Pmin and Pmax adjustable RS 232 port remote control connections Stop Flow Pressure Error 20 programs 9 program links WAKE UP program 100 240 V 50 60 Hz Maximum 40 W 5 3kg
14. the pump pis tons on the inside from jamming Legend A Capillary screw fitting B Outlet fitting 1 C Inlet fitting 2 D Fastening screw E Inlet fitting 1 F Outlet fitting 2 Fig 32 Screw fittings on the pump head Leaks in the capillary screw fittings Caution If leaks occur on the capillary screw fittings after maintenance and proper assembly do not tighten them further instead replace them with new connection capillaries Replacing the pump head Depending on the requirements of the user different pump heads are used Prerequisite The pump head has been purged with suitable solvent Aggressive or toxic solvent residue can irritate the skin Wear protective gloves Removing the pump head 1 Remove the tubes from the inlet and outlet of the piston backflushing A Remove all tubes from the solvent bottles 3 Unscrew the eluent line D Unscrew the outlet fitting 2 E and the inlet fitting of the pressure sensor F to remove the capillary 5 Loosen the opposite pairs of fastening screws B on the pump head evenly and alternately 6 Hold the pump head by hand and consecutively pull out all fastening screws 49 Maintenance and care 7 Remove the pump head Legend A Inlet and outlet of the piston backflushing Fastening screw Inlet fitting 1 Eluent line Outlet fitting 2 Inlet fitting of the pressure sensor Fig 33 Exchange the pump head Installing the pump head 1
15. was com piled based on research in the pertinent literature and is only a recommendation by the manufacturer In the event of doubt contact the technical support of the manufacturer To avoid damage from leaks always place solvent bottles in a solvent tray on the device Organic solvents are toxic above a certain concentration Ensure that work areas are always well ventilated Wear protec tive gloves and safety glasses when working on the device Connect silicon tube inner diameter 3 0 mm with the olive type tube fitting of the exhaust and lead the gases or liquids into a suitable collecting container or to a fume hood Organic solvents are highly flammable Since capillaries can detach from their screw fittings and allow solvent to escape it is prohibited to have any open flames near the analytical system Only use solvents that have a self ignition point higher than 150 C under normal ambient conditions The following solvents can attack the components of the pump and are therefore not suitable Mineral and organic acids except in buffer solutions Bases except in buffer solutions Liquids containing particles The following solvents are suitable to only a limited extent for use in the pump Methylene chloride Tetrahydrofuran THF Dimethyl sulfoxide DMSO Slightly volatile solvents Fluorinated hydrocarbons gt Regularly check for leaks and clogged capillaries test back pressure withou
16. 0 5 12 13 14 15 16 Enter the value for channel A O To confirm the entry press a horizontal arrow key Enter the value for channel B 100 To confirm the entry press a horizontal arrow key Position cursor on the diamond icon and press horizontal arrow key to exit the submenu Load Events offO on1JL2 Pressure 0 1MPa Flow Prog ml min Prog lt Link lt Hold 0 Run 1 Time A min B C D Events offO0 on1JL2 Pressure 0 1MPa Load Flow Prog ml min Prog lt Link Hold 0 Run 1 Time A min B C D Load Prog ml min Events offO0 on1JL2 Pressure 0 1MPa Flow Prog P01 1 000 0416 00000000 Setup Link a 000 0 100 O O O J GLP Hold 0 Time A B C D Run 1 min g Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog P01 new time gt Setup Link lt 000 5 gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog P01 1 000 0200 00000000 Setup Link a 000 5 0100 0 O j GLP Hold 0 Time A B C D Run 1 min Fig 20 Creating a program 35 Procedure Controlling the pump Running a program During the program sequence the changes in the solvent com position are displayed in the A D fields After the program sequence has been completed the display switches to END mode 1 Select the Program menu 2 Select the program number 3
17. 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog lt L01 gt Setup Link lt line01 P02 R01 WO GLP Hold 0 Time A B C D Run 1 min Fig 26 Creating a link 42 Procedure Controlling the pump Running a link After the link has been completed the pump stops The display switches to the END mode 1 Select the Link menu 2 Enter the link number 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Press START STOP to go to OFF mode 5 Enter program number then press horizontal arrow key the display switches to HOLD mode Press number 1 The display switches to HOLD mode 7 Press number 1 again The display switches to RUN mode linked program is running 8 After the linked program has been completed press START STOP The display switches to the OFF mode Load Flow Pressure Events Prog ml min 0 1MPa offO0 on1 JL2 Prog Open L01 pSetup Link lt aoe cece p GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO0 on1 JL2 Prog L01 gt Setup Link lt lineO1 PO2 RO1 WO J GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog P02 1 000 000 gt Setup Link lt HOLD 000 00 100 0 gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1 JL2
18. 59 Mixing chamber HPG system 23 Module safety 7 O Operation System 9 P PEEK 8 Piston backflushing 46 Power supply 9 Pump Controlling the GLP menu 31 Controlling the main menu 24 Controlling the Program menu 32 Controlling the Setup menu 25 Link menu of controller 40 Switch on and self test 23 Pump head exchanging 48 installing 49 Labeling 15 removing 48 Purging the pump 44 HPG or isocratic system 45 LPG system 44 R RFID recognition 19 64 S Safety 7 Screw fittings loosening 48 Self ignition point 8 Service see technical support 47 Solvent Degasser module 8 toxicity 8 unsuitable 8 Solvent tray 8 Solvents 7 Solvents suitable 7 storage 51 Symbols 10 T Target group 9 Technical data 56 Technical support 47 Toxicity Solvent 8 Transportation damages 59 Troubleshooting 52 U UHPLC system Operation 9 Unsuitable solvent 8 W WAKE UP program 39 Warnings 7 Warranty 59 Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH All rights reserved The information in this document is subject to change without prior notice Translation of the original German edition of this manual version 1 1 2013 01 04 Printed in Germany on environmentally friendly paper from sustainable forests ChromGate and ClarityChrom are registered trademarks of Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH gt See up to date manuals online www knauer net downloads
19. Prog P02 1 000 000 gt Setup Link RUN 000 16 73 17 p gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO0 on1JL2 Prog P02 1 000 000 gt Setup Link lt END 000 50 10 90 p gt GLP Hold 0 Time AK B C D Run 1 min Fig 27 Executing a link 43 Controlling the pump Deleting a link Select the Link menu Enter link number 0 Procedure 1 2 3 Press horizontal arrow key 4 Enter number of the link to be deleted and press horizontal arrow key to confirm the entry i Press vertical arrow key to confirm the query 6 To cancel the delete process press any number key Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog Open LO 01 02 03 Setup Linka gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog Delete program L gt Setup Link lt gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog Delete program L01 gt Setup Link lt confirm with cursor key gt GLP Hold 0 Time A B C D Run 1 min Fig 28 Deleting a link 44 Purging the pump Purging the pump Purging the pump in the LPG system The purging process of the pump is limited to a max pressure of 5 MPa If this value is exceeded during the purging process the pump switches off automatically In the Setup menu of the p
20. Screw in the opposite pairs of fastening screws B evenly and alternately Tighten all fastening screws evenly with an Allen key 3 Screw in the capillary with outlet fitting 2 E and inlet fitting pressure sensor F and tighten with a wrench Exchanging the ball valves Note The ball and position of the valves have been harmo nized to each other Insert the valves in the direction of flow Legend A Ball valve B Ball dotted line C Flow direction arrow Fig 34 Functional principle of the ball valve Dirty ball valves do not open and close correctly They cause pressure fluctuations and irregular flow DANGER Irritations of eyes and skin in case of contact with harmful solvents Purge the pump head with suitable solvent before changing the ball valve Do not use harmful solvent 50 Legend A Inlet fitting 2 B Capillary screw fitting C Outlet fitting 1 D Ball valve E Inlet fitting 1 Maintenance and care Removing the ball valves Note Loosen the screw fittings of the capillary connections alternately to prevent the capillaries from bending 1 Unscrew the inlet fitting 2 A and capillary fitting B 2 Unscrew outlet fitting 1 C 3 Remove the ball valve 4 Unscrew Inlet fitting 1 E 5 Remove the ball valve A B Cc D eo 50 ml eo E Fig 35 Exchanging the ball valves Cleaning the ball valves Note Water is to be used as solvent in case of impurities caused by s
21. ad Flow Pressure Events Prog ml min 0 1MPa offO on1 JL2 Prog a P 2 000 gt Setup Link lt OFF 000 00 100 0 p gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog a P 3 000 gt Setup Link lt OFF 000 00 100 0 gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog lt P 1 234 000 gt Setup Link BFF 000 00 100 0 gt GLP Hold 0 Time A B C D Run 1 min Abb 19 Setting the flowrate Creating a program The use of the display is demonstrated using the example of a program to be created Program 01 is to be used to create a solvent gradient channels A and B with constant flow rate of 1 ml min The percentage of solvent A is to be continuously decreased from 100 to 0 within 0 50 minutes 34 Procedure 1 2 Ww at O NO e Controlling the pump Select the Program menu Select the program number Press horizontal arrow key to go to the editing mode of the submenu Enter the value for the flow rate 01 00 To confirm the entry press a horizontal arrow key Enter the value for channel A 100 To confirm the entry press a horizontal arrow key Enter the value for channel B 0 To confirm the entry press a horizontal arrow key To specify the time in the submenu position the cursor on the diamond icon and press the vertical arrow keys Enter the time value 00
22. alt Methanol is to be used in case of impu rities caused by organic substances 1 Put the valve in a beaker with rinsing solvent for example methanol or isopropanol 2 Put the beaker in an ultrasonic bath for at least 10 minutes Installing the ball valves 1 Insert both ball valves 2 Screw in the outlet fitting 1 C and tighten to 15 Nm with a torque wrench 3 Screw in the inlet fitting 1 E and tighten to 15 Nm with a torque wrench 4 Screw in the inlet fitting 2 A and capillary fitting B and tighten with a wrench 51 Clean display Environmental protection Cleaning and caring for the device Risk of electrical shock or short circuit if cleaning solution enters the device s interior Moisten the cleaning cloth only slightly All smooth surfaces of the device can be cleaned with a mild commercially available cleaning solution or with isopropanol The display of the devices can be cleaned with isopropanol and wiped dry with a soft lint free cloth Environmental protection Disposal Drop the devices off at the local municipal waste facilities or send the devices back to the manufacturer where it will be dis posed of properly Decontamination Storage Contamination of devices with toxic infectious or radio active substances poses a hazard for all persons during operation repair sale and disposal of a device Danger caused by toxic infectious or radio active substances Never let a contam
23. amp 35 4 4 ine eV Wes oe PNR Ae ot a eae he aS 41 Executing a NK vest tea at a e s eye eG 2 end ee ea en ne ee 42 Deleting alin oserei ek eve took AN RAGS eat a eR oe toh we sea es 43 Purging the pump in the LPG system 005 44 Purging the pump in the HPG system 000 45 Connectors for piston backflushing eee eee 46 Screw fittings on the pump head 0 000005 48 Exchange the pump head 5 20 sash wen ewes be Deer eee wes 49 Functional principle of the ball valve 004 49 Exchanging the ball valves c2 00 604444045 bene bee bee ae 50 63 Index A Abbreviations 61 ambient conditions 51 B Ball valves cleaning 50 exchanging 49 installing 50 removing 50 C Care 51 Chromatography software 19 Cleaning 51 D Dead volume 9 Declaration of conformity 60 Decontamination 51 Delivery program 57 Disposal 51 E Electrical connections 16 Events terminal strip 18 Remote terminal strip 17 Snap ferrite interference suppression ring 16 Eluent line 20 Connecting the pump head 21 Environmental protection 51 F Features 13 Filter 9 Fittings tightening 47 Flammability 8 Flashpoint 8 G Gradient grade filtered solvents 9 Installation 11 Intake manifold LPG system 22 L Laboratory use 6 LAN 6 Leaks on capillary screw fittings 48 M Mains connection 9 Maintenance by user 47 Maintenance contract 47 Manufacturer
24. ays Also inform the freight carrier about transport damage Fastening material and shipping boxes The device is fixated and protected by foam inserts at the top and bottom Please keep the transport box and the foam inserts gt Remove the foam insert on the top of the device gt Grip the device at its sides near the front panel and lift it out of the packaging Protective film on the display During transport a protective film prevents scratches to the screen of the device Remove the protective film from the display 12 Use original parts and original accessories European hotline E mail Installation Scope of supply Pump with installed pump head Accessories Manual Cables Power supply cable Network cable RS 232 port cable 2 x flat ribbon cable 10 pos Set of connector strips 12 pos Kit for bleeding the pump Tools Silicon tubing Single use syringe 10 ml Fitting 1 8 10 x Seal ring 1 8 PTFE eluent filter Stainless steel eluent filter gt Only use original parts and accessories made by KNAUER or a company authorized by KNAUER Checking the scope of supply 1 Check whether the device and accessories are complete 2 If anything is missing consult with KNAUER Technical Support Hotline of KNAUER Technical Support Languages German and English Available by telephone 8 a m to 5 p m CET Phone 49 0 30 809727 0 Fax 49 0 30 8015010 E mai
25. dit the data using the chromatography software Check the pump Check the number of program lines A maximum of 100 program lines are possible Check the pump Check the number of program lines A maximum of 100 program lines are possible Change the entry Modify the program or replace the pump head Create and edit a program Quit program or wait until program has been completed The setup data can only be edited when no program has been loaded or started First unload the program First quit or delete wakeup program wu wakeup then edit or delete link First pause or delete the link then edit or delete data by means of the chroma tography software First quit or delete wakeup program wu wake up then edit or delete data by means of the chromatography soft ware Correct the time entry Edit the program line Check the number of program lines A maximum of 100 program lines are possible Check the pump head Check whether a pump head with RFID recognition has been installed 56 Technical data System message used CRC failed Wrong Line number Wrong link Technical data Ambient conditions Smartline Pump 1050 Temperature range Air humidity Conveying system Flow rate range Maximum pressure Flow rate accuracy Flow rate precision Solution Switch the device off and on If the system message appears again
26. e Controlling the pump Changing a program When values are entered the old values are overwritten 1 Select the Program menu 2 Select the program number 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Enter the desired value 5 Position cursor on the diamond icon and press horizontal arrow key for approx 5 s to exit the submenu Fig 22 Load Flow Pressure Events Prog lt Link lt Prog ml min 0 1MPa offO on1JL2 P01 01 pSetup Sey Se Ss ce p GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 P02 1 000 0416 00000000 Setup 001 0 1090 0 O j GLP Hold 0 Time A B C D Run 1 min Changing a program 37 Procedure Controlling the pump Deleting program lines 1 Select the program menu and enter the program number 2 Press horizontal arrow key to go to the editing mode of the submenu 3 Move the cursor into the program line to be deleted Press arrow key until the displayed time value is reached and change it into 0 000 5 Press horizontal arrow key Confirm the 7 Tocancel th Prog lt Link lt Prog lt Link lt Prog lt Link lt lt Prog lt Link lt query Press vertical arrow key e deletion process press any number key Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Open POI OT aS na esse ee Hold 0 Ti
27. e front of the device The pump head pressure sensor and connections for the mixing chamber and tubes are behind the door Device door with display and keypad The displays shows the pump and program status Use the START STOP key to start the pumping procedure Use the number keys to enter values and to control program sequences Use the PURGE key to activate the purge function of the pump 14 Practical tip Legend A Display B Key START STOP C Number keys D Key PURGE E Arrow keys F Standby key with LED Legend A Fastening points of the mixing chamber B Pressure sensor C Bleed screw D Tube connections for the piston back flushing E Pump head Startup Use the arrow keys to navigate horizontally and vertically through the menus Use the Standby key to set the pump to energy saving mode With the vertical arrow keys you can increase and decrease the displayed values Note To disconnect the pump from the mains power press the power switch on the rear of the device 1 KNAUER nmg nw Fig 1 Device door with display and keypad Front view of the device with connections Fig 2 Front view of the device with connections 15 Legend A Labeling on pump head without inlays stainless steel B Labeling on pump head with titanium inlays Legend A Serial number B Smartline Manager 5050 connection C Terminal strip Events Testing seal Warning symbol LAN connect
28. erscore character 1 Use the horizontal arrow keys to navigate through the two lines of the display 2 Use the number keys to enter a value 3 To confirm the entry press a horizontal arrow key Note If the entry is not confirmed the unchanged previous value will be displayed after approximately 2 seconds 4 Move the cursor onto the diamond icon to navigate through the subitems of the Link menu by means of the vertical arrow keys 5 Press START STOP or hold horizontal arrow key for 1 second to return to the main menu With the vertical arrow keys you can increase and decrease the displayed values Here also the maximum pressure for the program can be changed The maximum pressure can differ from the default one and is thereby only valid for this program 33 Controlling the pump Setting the flowrate Note When setting the flowrate the complete digit sequence will be displayed after all digits have been entered Example Setting the flowrate to 1 234 ml min 1 Enter 1 2 3 and 4 successively by using the number keys 2 To confirm the entry press a horizontal arrow key Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog lt P 8 000 000 gt Setup Link a OFF 000 00 100 0 gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1 JL2 Prog lt P 1 _ 000 gt Setup Link OFF 000 00 100 0 gt GLP Hold 0 Time YA B C D Run 1 min Lo
29. es 26 Parameters of the Setup Menu pate ce os aac Reed obs Pe Bee y 28 Controlling the GLP menu vasss game doe eae Raa ak eh we eR Mek 31 Structure of the GLP menu 14 2 204 2h a user eae So oes ee eee Se es 31 Controlling the Program menu ce eee eee eet eee ee eens 32 Setting the flowrate oa a2 gate dew PA a HEM ES RIGA RI ERs 33 Creatingia progran ni athe nace bg ois ite Asis eed wy eked Badin hag no eee 33 RUMMING a progam sor wa twas Le Ss BOTAN Ros Ea OGRE Roe ees 35 Changing a program eite s sorie p ieee hie nea DERE Re ad REE OS 36 Deleting programslinesi aa 0 066s 6 oe eas awa Sah Ieee TRG wah ees 37 Deleting a Program os ce es eid soe ini a io Sadr Baa hed 38 Creating a program with preselectable start time 4 39 Controlling the Link menu ss vss eaese VaR Gees ees ee epee daw 40 Creating a link seire misanan Ree Ow hd Hees Marea PRS TAN wee 41 RUMNING A IINKS 252 oe actda nd Ah Souls aaa as eu IS Ae ee Oe aes 42 Del tinga link sarir eraen o a E ER oa sen a a e ae EE e Kass 43 Purging th PUMP n rere bk heed dep a eaim OR ER eRe OS Ae Eee e 44 Purging the pump in the LPG system 0 eee eee eee eee ee 44 Purging the pump in the HPG or isocratic System aun na warts are acti aana aie laa pha wie Sie ano S phe alre Sie Weare aie 45 Piston backhUushing s see eee em E wee He ae ee OSE ee RRS 46 Maintenance and care cee ee ee eee eee teens 47 How to contact KNAUER Technical
30. ey have been used never re use capillaries in other areas of the HPLC system 2 Only use a given PEEK fitting for one specific port and never re use it for other ports Always install new PEEK fittings on each separate port gt Use special columns and follow the manufacturer s instruc tions on caring for the columns gt Regularly check for clogged capillaries test back pressure without column 1 Use ultra pure filtered solvents Gradient grade for the HPLC Filtration of substances to be analyzed 3 Use of inline filters 10 Symbols and labels Devices are to be Note opened by the technical support department only Only allow the technical support department of the manufacturer or a company authorized by the manu facturer to open the devices for maintenance and repair work What expertise should users have to safely operate a HPLC device or device system Comp parabl leted degree as chemical laboratory technician or com e vocational training Fundamental knowledge of liquid chromatography Participation in an installation of the system performed by the manufacturer or a company authorized by the manufacturer or suitable training on the system and chromatography soft ware Basic knowledge of Microsoft Windows Knowl edge regarding substances that are suitable only to a limited extent for use in liquid chromatography Symbols and label S Explanations of the symbols a
31. ff 0 B GLP menu A C Link menu B Time D Program menu un 4 min Fig 16 Controlling the main menu 25 Procedure Procedure Procedure Procedure Navigating through the Setup menu Controlling the pump Selecting the Setup menu 1 Use the horizontal arrow keys to put the cursor in the top line of the display Press the right horizontal arrow key for approx 5 seconds Press START STOP or hold horizontal arrow key for 5 seconds to return to the main menu Selecting the GLP menu 1 Use the horizontal arrow keys to put the cursor in the lower line of the display Press the right horizontal arrow key for approx 5 seconds Press START STOP or hold horizontal arrow key for 5 seconds to return to the main menu Selecting the Link menu 1 Use the horizontal arrow keys to put the cursor in the lower line of the display Press the left horizontal arrow key for approx 5 seconds Press START STOP or hold horizontal arrow key for 5 seconds to return to the main menu Selecting the Program menu 1 Use the horizontal arrow keys to put the cursor in the top line of the display Press the left horizontal arrow key for approx 5 seconds Press START STOP or hold horizontal arrow key for 5 seconds to return to the main menu Controlling the Setup menu In the Setup menu fundamental parameters for controlling the pump are specified Use the horizontal arrow keys to navigate through the two
32. h the following requirements and prod uct specifications Manufacturer name and address Smartline Pump 1050 DIRECTIVE 2006 42 EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 17 May 2006 on machinery and amending Directive 95 16 EC recast IEC 60799 1998 Electrical accessories Cord sets and inter connection cords IEC 61010 1 2010 Corrigendum 2011 Safety require ments for electrical equipment for measurement control and laboratory use Low voltage directive 2006 95 EC EN 61000 3 2 2005 A1 2008 A2 2009 Electromagnetic compatibility EMC Part 3 2 EMC standard 2004 108 EC IEC 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements EN 61326 1 Corrigendum 2 2011 Directives for an environmentally sound use of electrical and electronic equipment RoHS directives 2002 95 EC 2003 and 2011 65 EU on the restriction of the use of certain hazardous substances in electrical and electronic equipment WEEE directive 2002 96 EC 2003 on waste electrical and electronic equipment The product was tested with a typical configuration Berlin 10 10 2012 Aye Dr Alexander Bunz Managing Director The mark of conformity has been applied to the rear panel of the device CE 61 Abbreviations and terminology Abbreviations and terminology Here you can find information on the abbreviations and terminol ogy used in this manual Terminology GLP Grad
33. he degassing module in the Smartline Manager 5050 LPG system Connect the solvent reservoir to the pump via the degassing module in the Smartline Manager 5050 HPG system Connect the respective solvent reservoir to sev eral pumps via a degasser or the degassing module in the Smartline Manager 5050 Note KNAUER recommends always using a degassing mod ule to ensure reliable transport of the solvent as eluent 21 Connecting the pump with other devices Connecting the eluent line in the isocratic system or HPG system to the pump head gt Make sure that the tapered side of the cutting ring is pointed towards the fastening screw of the Teflon tube Connecting the eluent Steps Figure line to the pump head 1 Push the Teflon tube D through the fas tening screw C and the cutting ring B 2 Insert the tube end as far as possible into the inlet fitting A of the pump head 3 Tighten the fastening screw by hand Fig 10 Connecting the eluent line at the pump head 22 Legend A Knurled head screw B Filler cap C Mixing unit Connecting the intake manifold Connecting the pump with other devices Intake manifold Connect up to four outputs of the LPG module of the Smart line Manager 5050 to the intake manifold inlets at the pump head To avoid the discharge of liquid out of the analysis system close unused inlets by means of filler caps Fig 11 Intake manifold with four inlets
34. ient HPG HPLC IP address Link Solvent LPG Manager Remote Explanations Good Laboratory Practice quality assurance for laboratories Time dependent composition of solvent mobile phase on low or high pressure side of system High Pressure Gradient HPG Operating mode of an HPLC or UHPLC system The solvent is mixed on the high pressure side of the pump High Performance Liquid Chromatography HPLC Unique address of transmitter or receiver in local network or Internet Internet Protocol Combination of multiple chromatography programs in a single HPLC system Mobile phase eluent carrier for liquid chromatography Low pressure gradient LPG Operating mode of an HPLC or UHPLC system The sol vent is mixed on the low pressure side of the pump Combined device consisting of degassing module gradient module and analog to digital converter The chromatography software controls the pump Table O Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig f fi ea ee Poe WWWWWWNNNNNNNNNN FSH SRP SBBsaanyo SE OF DO eae SO OO OS St OP Nr O SO Os ON es Ne ee 2 gures Device door with display and keypad 4 14 Front view of the device with connections 14 Labeling on the pump heads
35. ih KNALIER ar thine gt Pump 1050 Manual V7608A 000 3 Table of contents Note For your own safety read the manual and always observe the warnings and safety information on the device and in the manual OVEIVICW 520 ovine wee SSW Wg E aS peed Magee ea ewes 6 PUMP TYPE arriu dbo ee ee Bae Ae ae Ue bee ae we em ba es 6 Local area network and automatic configuration 2 0 ee eee 6 Laboratory se ac is we eee aS eon eS PENSE ewe ONE OS WENO 6 Where is it prohibited to use the device or system 00 ee 7 Safety lt td sel RR OLE ORES OO EOS ERE ARE HERE REELS 7 Laboratory regulations a2cavaquietuwas ta aU de VO HAT SECT R oes ORES 7 SOWGNUS cic esiu bax ie ewes Ba tutte we Sete ek owe te Rees eR eee ot 7 PEEK CONMGCUONS lt 0 ata eile eat E dada Ciclo aero aa at Pi alee ase led sana 8 Protective measures wicas avGue 6a Os Tea hw oS A ee dee 2G Kowa Se 9 Power supply and mains connection 0 0 eee eee eee 9 Target group esda en iat Giark als a ete es bates suk as oe daa 9 Operating the device or device system 0 eee ee eee 9 To what should the user pay particular Attention 3g dine ce iin Sates aes whale DE ge Ae ge ad ooh a 9 What expertise should users have to safely operate a HPLC device or device sys COME orae PEG Oh la ce E Pl ap enced ere GE Uae ate arn 10 Symbols and labels 3053p ghee eae Ae BO OP EA ERR OSI 10 Installation io sales do aretha Sse wea e s em es
36. inated device be repaired sold or dis posed of Commission a specialized company with the decontamina tion or perform it yourself if you have the required special ized know how All contaminated devices have to be decontaminated properly by a specialized company or the operating company before these may be recommissioned repaired sold or disposed of All materials or fluids used for decontamination must be collected separately and disposed of properly Ambient storage conditions for the device Temperature range 4 40 C 39 2 104 F Air humidity Below 90 humidity non condensing 52 Troubleshooting Troubleshooting First measures for troubleshooting Check all screw fittings Check whether air has gotten into the supply lines Check device for leaks Further measures Check errors against error list Contact the technical support hotline of the manufacturer Error list and solutions Problem Pump will not turn on When purging the pump switches off Pump does not transport solvent Pressure or flow rate fluctuations Solution The power cable must be connected to the power supply gt Inspect the power cable to ensure that it is plugged into the power supply The ventilation screw on the pressure sensor must be turned up gt Check if the ventilation screw on the pressure sensor is turned up Check the following options Purge the pump head to remove the air bubbles
37. ion RS 232 port Fan opening IO Tn mg Terminal strip Remote J Power switch K Mains power connection L CE mark Startup Labeling on the pump heads The front of the pump head is labeled with the max pumping capacity 10 ml or 50 ml Pump heads with inlays are addition ally labeled with the respective material Ti for titanium Fig 3 Labeling on the pump heads Rear view of the device The rear of the device contains the mains power connection power switch connections for external devices symbols warning signs and serial number Fig 4 Rear view of the device with connections 16 Connecting the pump with other devices Connecting the pump with other devices Legend A Flat ribbon cable B Ferrite ring Electrical connections Use the Events and Remote terminal strips to connect the pump with external devices Use the LAN connection to connect the pump with external devices within a network Alternatively connect the pump to a computer by means of the RS 232 port Use the special connection cable to connect the pump and Smartline Manager 5050 Caution Electrostatic discharge can damage the electronics of the pump Never touch the electronic contacts of the Events and Remote terminal strips Attaching the snap ferrite Flat ribbon cables that are connected to the Events and Remote terminal strips can sometimes cause electromagnetic interfer ence This can be supp
38. kale omega al Gals Raver E e 11 Packaging and transport 04 42 2cene renin sae ee tees Ree KARR 11 Fastening material and shipping boxes 2 cece eee ee eee 11 Protective film on the display 0 0 0 cee eee eee eee eee 11 SCOPEOFSUDDIV ch yee s ogee teehee Pawo ees ee iE See eee es oe he 12 ACCESSONIES ch Pate thee he Oe Nee t he Slay GUE itt ee tal ls a hg 12 Checking the scope of supply 2 0 ee eee ee eee 12 Space reguirements ses gaa sss tenn ha Sheen eee ee nas Ye ye tuck as 12 INStANAUONSUE arasin redan ra ea TE hoes se bor eel as aia esos hes 13 Feat res o reiesme a ea th ie le Rook Si hE A Win a Sk Wo Be yO A a SER Se ak kel 13 Startup anii be rcs go aaa Ee E Sharon ars Paw AR age he Ao ar Peck na ae tech TS 13 Front View of the device 7 nd ace x6 eR Re RAR OOS EERE RRR ES 13 Device door with display and keypad 20 e ee ee eee 13 Front view of the device with connections 000000 14 Labeling on the pump heads eee eee eee eee eens 15 Rear View OF th e device cos swine eecu saree Behe BARE EDE AGRE EEG Ys 15 Connecting the pump with other devices 000 0000s 16 Electrical connections vs a2 suv Zoaek Boe ede bates a eee Ve aad he 16 Attaching the snap ferrite 0 ev cdt eet iaahr eu eRe ALE REN eee RSG 16 V7608A Connecting the flat ribbon cable with the CONHECIOl SUID ios heated eae xe SERRE RRR PORE REG eRe a 16 Terminal strip
39. l info knauer net Space requirements Side clearance to other devices At least 5 cm if there is another device on one side At least 10 cm if there are other devices on both sides At least 30 cm gap to the fan on the rear of the device 13 Ambient conditions of the installation site Features Startup Features Installation site Air humidity below 90 non condensing Temperature range 4 40 C 39 2 104 F Sunlight When setting up the system at the installation loca tion make sure that it is protected against direct sunlight Dual piston technology combined with optimized electronic pulsation dampening and compressibility compensation Liquid transport with low pulsation stable flow rate and high flow accuracy Long service life Pump head made of stainless steel or with titanium inlays Automatic piston backflushing Internal controlling for programming binary to quaternary high pressure gradients In the high pressure gradient system up to four pumps can be combined In the low pressure gradient system in conjunction with the Smartline Manager 5050 up to four eluents can be combined The processes can also be controlled directly by the pump without chromatography software by means of analog and digital signals Control with KNAUER chromatography software such as ChromGate or ClarityChrom Front view of the device The input field and display of the pump are located in the door on th
40. lines of the display Use the vertical arrow keys to select the desired option Move the cursor onto the diamond icon on the left in the lower line to navigate through the subitems of the Setup menu by means of the vertical arrow keys Use the number keys to enter a value Press START STOP or hold horizontal arrow key for 5 seconds to return to the main menu 26 Controlling the pump Structure of the Setup menu INTERFACE LAN DHCP Analog RS232 9600 RS232 19200 RS232 38400 RS232 115200 LAN manual 19 01 2010 d m y 13 03 55 h m s PUMP HEAD Auto 50ml f 10ml PRESSURE LIMITS 0 1MPa min 000 max 400 HPG D HPG C HPG B HPG A LPG GRADIENT MODE none ID 0001 ON ON ON all LPG CYCLE TIME 1s 2s 5s 4s 3s SOLVENT Compressibility 0 34 disabled START IN enabled start pump stop pump 27 Controlling the pump 24V E 8 E 7 E 6 E 5 EVENTS active start 0 error 0 m ANALOG OUT pressure range 1V SEn ANALOG OUT OFFSET 0000 00mV f PRESSURE OFFSET 00 00MPa f Disabled HPG LPG D HPG LPG C HPG LPG B HPG LPG A Slave B 2V 5V ANALOG IN set zero actual 00 00 ml t 10s 5s TIME CONSTANT analog 2s 10s in no out no 1s 5s 05s 2s 0 2s 1s 0 5s 0 2s Activate edit events 00000000 DISPLAY Brightness 2075 Contrast 0741 Fig 17 Structure of the Setup menu 28 Controlling the pump Parameters
41. mail Proper maintenance of your HPLC device will ensure successful analyses and reproducible results How to contact KNAUER Technical Support If you have any technical questions regarding KNAUER hardware or software please use one of the contact options below Hotline of KNAUER Technical Support Languages German and English Available by telephone 8 a m to 5 p m CET Phone 49 0 30 809727 0 Fax 49 0 30 8015010 E mail info knauer net Maintenance contract The following maintenance work on the device may only be per formed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract gt Opening the device or removing housing parts What maintenance tasks may users perform on the device Users may perform the following maintenance tasks themselves Replacing the pump head Exchanging the ball valves Tightening the screw fittings 1 Always tighten the inlet screw 1 E and the outlet screw 1 B with a torque wrench and 15 Nm 2 Always hold the inlet fitting 1 E with a wrench when the capillary fitting A is being tightened with a wrench 3 Using an Allen wrench screw in the opposite pairs of fasten ing screws D evenly and alternately to prevent the pump pistons on the inside from jamming 48 Maintenance and care Loosening the screw fittings gt Using an Allen wrench loosen the opposite pairs of fastening screws D evenly and alternately to prevent
42. me A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 P02 1 000 0416 00000000 Setup 000 5 0 100 0 O j GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 P01 1 000 0416 00000000 Setup 0 000 0100 0 O J gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Delete prog line 02 gt Setup confirm with up down key gt GLP Hold 0 Time A B C D Run 1 min Fig 23 Deleting program lines 38 Procedure Controlling the pump Deleting a program 1 Select the Program menu Enter program number 0 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Enter number of the program to be deleted and press hori zontal arrow key to confirm the entry 5 Press vertical arrow key to confirm the query Load Flow Pressure Events Prog ml min 0 1MPa offO on1 JL2 Prog Open P0 01 pSetup Link lt eee cece GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1 JL2 Prog lt Delete program P gt Setup Link lt gt GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1 JL2 Prog Delete program P01 gt Setup Link confirm with cursor key gt GLP Hold 0 Time A B C D Run 1 min Fig 24 Deleting a program
43. menu Controlling the pump Controlling the GLP menu In the GLP menu statistical data on important parameters of the pump are displayed A diamond icon is displayed on the left of the lower line in the display Move the cursor onto the diamond icon to navigate through the subitems of the GLP menu by means of the vertical arrow keys Press START STOP or hold horizontal arrow key for 5 seconds to return to the main menu Structure of the GLP menu GLP_P S N AAA091800007 version 01 01 GLP_P volume 0002 21 p index 125041 0 MPah GLP_P cycles 196406 total time 00066 6h instal 01 07 2009 service 18 01 2010 GLP_H S N PAB head type Auto ml GLP_H volume 0001 91 p index MPah GLP_H cycles 000000 total time 00000 0h GLP display D01 02 T00 52 Fig 18 Structure of the GLP menu 32 Navigating through the Program menu Practical tip Controlling the pump Controlling the Program menu The Program menu is used to list and create programs The pump can save max 20 programs programs and program links and max 100 program lines Max nine program links between defined programs can be created and saved Programs have the following functions Controlling the flow rate Controlling the solvent composition Sending control signals Events A diamond icon is displayed on the left of the lower line in the display During data entry the cursor turns into the und
44. nd labels on the Pump 1050 Symbol Explanations Hazard symbol indicating microelec tronic devices that can be damaged by electrostatic discharge when touched Flow direction symbol for piston back A flushing inlet to flushing pump Flow direction symbol for piston back flushing outlet from flushing pump Marking for devices that comply with the canadian requirements for labora jd tory equipment CAN CSA C22 2 No 61010 1 second edition inclu ding Amendment 1 or a later version 11 Installation Checking for signs of damage during transport Removing the fixation material Removing device from the packaging Removing the protective film Installation Testing seals in Canada and the USA at nationally recognized testing centers NRTL The certified device or system has successfully passed the quality and security tests A T V Rheinland CE Conformit Europ enne mark for equipment that complies with the per tinent EU directives and comes with a declaration of conformity from the manufacturer For your own safety read the manual and always observe the warnings and safety information on the device and in the manual Packaging and transport At the factory the device was carefully packed for safe transport Check the device for signs of damage that occurred during trans port If the shipment is incomplete or damaged inform the man ufacturing factory within three workd
45. nks Max nine pro gram links between defined programs can be created and saved A diamond icon is displayed on the left of the lower line in the display Use the horizontal arrow keys to navigate through the two lines of the display Use the number keys to enter a value Move the cursor onto the diamond icon to navigate through the subitems of the Link menu by means of the vertical arrow keys Press START STOP or hold horizontal arrow key for approx 1 second to return to the main menu With the vertical arrow keys you can increase and decrease the displayed values 41 Procedure Controlling the pump Creating a link Note Ifthe W7 W Wait setting is used the pump waits for an external start signal or for the user to press number 1 before executing this line If the WO setting is used the link sequence is run without interruption 1 Select the Link menu Enter the link number 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Move the cursor to position P in the display and enter the program number 5 Move the cursor to position R R Repeat and enter the value number of repetitions for the specified program 6 Move the cursor to position W in the display and enter 1 or 0 Load Flow Pressure Events Prog ml min 0 1MPa offO on1 JL2 Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog lt Link lt Hold
46. of the Setup menu Value INTERFACE IP adr mask IP GW Port DATE PUMP HEAD PRESSURE LIMITS GRADIENT MODE LPG CYCLE TIME Meaning Setting for external connection options of pump The connections are on the rear of the device Network configuration IP address Network Mask Network configuration Gateway Port Setting for the date and time Pump head selection The selection is used as basis for calculat ing the flow rate and determines the max system pressure This value applies for all operating modes and cannot be exceeded Settings for the min and max pressure The pump stops automatically if the pres sure drops below the min value or exceeds the max value The min pressure value is used to ensure that the pump does not run dry If the minimum is set 0 the minimum system pressure is not monitored Here the modes of the high pressure gra dient HPG low pressure gradient LPG and isocratic system can be set LGP activation of up to four channels HPG selection between pump name HPG A to HPG D Isocratic system none Time specification for the switching cycle of the valves of the Smartline Manager 5050 Factory preset is 2 seconds When working with higher flow rates and lower mixing chamber volumes reduce the time to achieve better mixing of the eluents a When working with lower flow rates and higher mixing chamber volumes increase the time to obtain mo
47. program or in setup Switch the device off and on If the system message appears again notify KNAUER Technical Support Device error change device settings Change the entry Change the entry Check the cable connections Check the validity of the parameter s Correct the time entry Change the entry in the program line First unload the link then change the link or delete it Wait until the link has been executed then change the link or delete it Reduce the pressure or adjust the upper pressure limit Restart the system Increase the pressure or adjust the lower pressure limit Restart the system Since the pump can only be operated using a specific valve the entered data has to be adapted to isocratic mode Select an existing link in the wake up program or create a link and edit it Create a link and edit it Edit the data by means of the chroma tography software Troubleshooting System message Non existing com ponent is set to non 0 value Not enough space to store link Not enough space to store program Not supported Prg not compatible with pump head Program does not exist Program is running The gradient com ponent is used this link is used in WAKEUP this program is used in a link this program is used in WAKEUP Time already exists Time table line is empty Too much lines in program Unknown pump head type Solution Switch on the channel or e
48. re precise mixtures 29 Controlling the pump Value SOLVENT START INPUT EVENTS Meaning Data entry for the compressibility of the selected solvent Water 0 46 Benzene 0 95 Chloroform 0 97 Ethanol 1 10 Acetone 1 23 Methanol 1 21 n heptane 1 40 n hexane 1 50 Diethyl ether 1 84 Acetonitrile 0 99 Tetrahydrofuran 0 93 Water methanol 10 90 1 17 Water methanol 20 80 0 86 Water methanol 40 60 0 56 Water methanol 50 50 0 52 Water methanol 60 40 0 46 Water methanol 80 20 0 40 Water methanol 90 10 0 34 After receiving a signal from an external device the device or a program start an automatic run Software sends a trigger signal via LAN There are 4 possible settings Enabled Prgram starts Disabled No program starts Start pump Pump starts a run Stop pump Running pump stops Used for testing the Events terminal strip Connections E 1 to E 8 and 24 V can be selected The lower line shows the sta tuses at the digital inputs 30 Controlling the pump Value ANALOG OUT ANALOG OUT OFFSET PRESSURE OFFSET ANALOG IN TIME CONSTANT ACTIVATE EDIT EVENTS DISPLAY Meaning Used to output analog pump signals to external devices by means of the Remote terminal strip on the rear of the device Pressure preset for outputting the currently measured pressure for the purpose of external recordings or con
49. ressed by means of a snap ferrite gt Open the snap ferrite place it around the flat ribbon cable and close it Fig 5 Attaching the snap ferrite Connecting the flat ribbon cable with the connector strip To enable signal transmission from external devices to the pump the flat ribbon cable is connected with a connector strip and con nected to the Remote terminal strip on the rear of the pump 17 Connecting the pump with other devices Connecting flat ribbon Steps Figure cable with connector strip 1 Place the connector strip C on a suitable surface R 2 Insert the stylus A into the opening on the top of the connector strip and press downward 3 Keep the stylus pressed E down and insert the cable ends B into the front of the connector strip i Remove the stylus or pher 5 Check whether the cables are tightly Fig 6 Connecting the flat attached ribbon cable with the connector strip Terminal strip Remote For receiving start control and error signals from external devices For sending start control and error signals to external devices 8 TE Ce een in a e S8x802828788 Fig 7 Remote terminal strip 18 Connecting the pump with other devices Terminal strip Remote Signal ANALOG OUT ANALOG IN START OUT START IN ERROR OUT ERROR IN Explanations Analog output signal Either outputs the measured system pressure or a control volt age for Pump B
50. s 7 Safety Adherence to laboratory regulations Suitable solvents Safety Where is it prohibited to use the device or system DANGER Risk of explosion Never use the device in potentially explosive atmospheres without appropriate protective equipment and approval by a notified body Inform the technical support department of the manufacturer Laboratory regulations gt Observe national and international regulations pertaining to laboratory work Good Laboratory Practice GLP of the American Food amp Drug Administration For development of methods and validation of devices Protocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory American Journal of Medical Technology 44 1 pages 30 37 1978 Accident prevention regulations published by the accident insurance companies for laboratory work Solvents Solvents suitable for use in HPLC Acetone Acetonitrile Benzene Chloroform Acetic acid 10 50 at 25 C 77 F Ethyl acetate Ethanol Hexane heptane Isopropanol Methanol Phosphoric acid Toluol Water 8 Solvent tray Toxicity Valid for degasser module Flammability Self ignition point Unsuitable solvents Suitable to only a limited extent Leaks and clogged capillaries Safety Note Even small quantities of other substances such as addi tives modifiers or salts can influence the durability of the materials The list of selected solvents
51. s part of a high pressure gradient system HPG or low pressure gradient system LPG by means of a computer and the ChromGate or Clarity Chrom chromatography software by KNAUER The pump uses an open control protocol Therefore other soft ware can also be used to control the pump RFID recognition The pump head is equipped with an RFID chip It is used to mon itor and save all important parameters and settings of the pump and pump head In the display a diamond icon designates the RFID function 1 Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog P 00 75 000 gt Setup Link OFF 000 00 100 O O O Jj GLP Hold 0 Time PK WB C D Run 1 min Fig 9 RFID designation in the pump display 20 Connecting the pump with other devices The RFID technology offers the following advantages Automatic detection of all important pump head parameters by means of radio signals radio frequency identification by the pump software or the chromatography software Pump head type Serial number and year of manufacture Number of cycles and operating times Limit values of the pump head parameters Storage of all measuring data in accuracy with GLP Good Lab oratory Practice Transfer of all data to the software and pump display Eluent line Make sure that all connections are tight Isocratic system Connect the solvent reservoir to the pump either directly via a degasser or t
52. sistant up to 400 bar If bio compatibility is required titanium inlays can be used The pump heads can be exchanged easily Automatic piston backflushing increases the service life of the seals and pistons The piston backflushing removes salt and other substances from the area behind the seals Note Due to the new drive used in the Smartline Pump 1050 pump heads of previous models cannot be used any more Pump type For an initial startup of the pump liquid has to be sucked in via a syringe Local area network and automatic configuration The pump is controlled either by means of the input field on the front of the device or by means of the chromatography software Normally the pump is controlled by means of the chromatogra phy software via a local network LAN The pump automatically recognizes the pump head by means of an RFID chip The pump connected in the local area network LAN is automat ically recognized by the chromatography software When used in a local area network LAN the system status of the pump can be verified by means of chromatography software Ex works the pump is set to DHCP Dynamic Host Configuration Protocol This means that the pump is automatically assigned an IP address within the local network In the Setup menu this set ting can be modified manually Laboratory use Biochemistry analyses a Chiral analyses Food analyses Pharmaceutical analyses Environmental analyse
53. t column PEEK connections Disposable PEEK fittings One piece disposable polyetheretherketone fittings PEEK for easier installation of flexible thin capillaries Tightening torque of the PEEK screw Tightened by hand approx 0 5 Nm 9 Avoiding additional dead volumes Using special columns Checking for clogged capillaries Using filtered solvents Safety Protective measures 1 You are only permitted to perform the maintenance tasks described in this manual 2 All other maintenance tasks are to be performed exclusively by KNAUER or a company authorized by KNAUER Without exception the following applies to all maintenance tasks that can be performed by the user 1 Switch off the device and pull the power plug 2 Never open a device High voltage poses a life threatening risk Power supply and mains connection The device is intended for use with AC power networks of 100 240 V The supplied power cable is to be used to connect the device to the mains supply Note To disconnect the pump from the mains power disconnect the power plug on the rear of the device Target group Operating the device or device system The device can be operated by means of the chromatography software at the workstation or the membrane keyboard at the device To what should the user pay particular attention To make your HPLC separations as efficient as possible pay close attention to the following 1 Once th
54. the display The device performs a self test After the test has been successfully completed the SYSTEMTEST OK message appears for a short time After that the program that was active last will bee displayed The pump is now ready for operation 1 Switch on the pump 2 Wait until the pump has completed the self test 24 Controlling the pump Display and keypad Legend A Display Program i 7 a A B C D Display Flow rate A Pressure Events 0 1MPa offO ont m2 Display Pressure Event status Arrow key Standby key Display Gradients L ZTaA TM ON BB Number key Display Time J Status of pump or program or link K Key PURGE J H G F L Key START STOP Fig 15 Display and keypad Controlling the main menu The main menu contains the current parameters of the pump From here the Setup GLP Program and Link menus can be accessed During navigation through the display the cursor is a flashing rectangle Navigation through 1 Use the horizontal arrow keys to navigate through the two the main menu lines of the main menu s display 2 Use the number keys to enter a value 3 To confirm the entry press a horizontal arrow key Note If the entry is not confirmed the unchanged previous value will be displayed after approximately 2 seconds Practical tip With the vertical arrow keys you can increase and decrease the displayed values Legend A Setup menu Flow Pressure Events ml min 0 1MPa o
55. thorized changes are made to the device During the warranty period any components with material or design related defects will be replaced or repaired by the manu facturer free of charge This warranty excludes the following 1 Accidental or willful damage 2 Damage or errors caused by third parties that are not con tractually related to the manufacturer at the time the damage occurs 3 Wear parts fuses glass parts columns light sources cuvettes and other optical components 4 Damage caused by negligence or improper operation of the device and damage caused by clogged capillaries 5 Packaging and transport damage In the event of device malfunctions directly contact the manu facturer Manufacturer Wissenschaftliche Geratebau Dr Ing Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 E Mail info knauer net Internet www knauer net Transportation Damages The packaging of our devices provides the best possible protec tion against transportation damage Check the devices for signs of transportation damages In case you notice any damage con tact the technical support and the forwarder company within three workdays 60 Declaration of conformity Declaration of conformity Wissenschaftliche Geratebau Dr Ing Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin Germany Product number A50351 1 A50353 1 A50361 A50363 The device complies wit
56. trol Slave B preset for outputting a signal for controlling a second pump Presets HPG LPG A to HPG LPG D Voltage range options 1 V 2 V 5 V OFFSET data entry for the analog output Displays the automatic offset adjustment for the pressure display Make sure that the bleed valve is open Calibration option of the analog input of the Remote terminal strip for remote flow rate control At set zero an input voltage that is pres ent can be defined as zero point i e defined as a flow of 0 00 ml min by means of the vertical arrow keys If a devi ating positive control voltage is applied a resulting flow rate is displayed at actual based on the basic setting 1 ml corre sponds to 1 V for the 10 ml pump head and 5 ml corresponds 1 V for the 50 ml pump head This flow rate can then be changed by pressing the vertical arrow keys Thus a second calibration point is gener ated which modifies the scaling Selection of a time constant to be used for signal smoothing The larger the value of the selected time constant the stronger the applied signal smoothing Both an external signal present at the analog input in and a signal generated at the analog output out can be smoothed The E 1 to E 8 connections of the Events terminal strip can be activated 1 or deactivated 0 by means of the vertical arrow keys Here the brightness and contrast of the display can be set 31 Navigating through the GLP
57. ump select Gradient Mode LPG In purging mode select channel MIX or gradients A B C and D Note Before the purging process open the bleed screw to prevent pressure surge and damage to the column Procedure 1 Open the bleed screw 2 Suck liquid in via a Luerlock syringe 3 Press PURGE 4 Select the purging mode 5 Enter the value for the flow rate 6 Press START STOP to start the purging process 7 Press START STOP to stop the purging process Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog lt PURGE 3 000 000 gt 5MPa Setup Link lt lt OFF gt channel MIX j GLP Hold 0 Time A B C D Run 1 min Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog lt PURGE 3 000 000 gt 5MPa pSetup A Link a lt PURGE gt channel MIX jJ GLP B Hold 0 Time A B C A D C Run 1 min D Fig 29 Purging the pump in the LPG system 45 Procedure Purging the pump Purging the pump in the HPG or isocratic system In the Setup mode of the pump select Gradient Mode HPG A HPG B HPG Cor HPG D or none In purging mode set the values for the flow rate 1 Open the bleed screw 2 Suck liquid in via a Luerlock syringe 3 Press PURGE 4 Enter the value for the flow rate 5 Press START STOP to start the purging process 6 Press START STOP to stop the purging process Load Flow Pressure Events Prog ml min 0 1MPa offO on1JL2 Prog lt PURGE 3 000 000
58. v esos eke eke OWES SAS SH RSS OE 53 Technical Gata ccs cbs ate slate eo RG eens te ewe nd eam naval ee a 56 Delivery pr gram Dervio pes were ea ae ace Re BR oe a bee Gedy oe 57 Devices and accessories 44 eo ee Se eseaateacwsa diab eh aad x 57 Spareparts as Aue Ae hee hea eg Beg beets heii bas a eae dal Ait Balke coded g 58 Power supply cable 2 029 Sesser See We ae a a eee ae 58 Legal Information os i526 oe eae Ea 4 Aw eG ORE EBR AES AG RR Hy ES 59 Warranty Conditions ss es eea ans od ama Ce o oa ae ua tah ewe ate Bas ae 59 ManGbaCluirets sia eeu aae Soria ise aya Gale Be E RCRD Stee tata Se dap 59 Transportation Damages 5 6 se2 cae 8 ES sae hed A RAS ER A SE ES 59 Declaration of conformity 2 eee ee eee eee 60 Abbreviations and terminology 2 eee eee eee eee 61 Table OF fIQUIES sr ithe ee BONA Sad Oe PAG ORT aS BH SS 62 Index ae erect ORM cH ne tr ce aT i Bee Me ET Ta ERI CEST RICE on RE 63 Overview HPLC Smartline Pump 1050 Non self priming pump Remote control Automatic pump head recognition Automatic configuration Device status LAN setting High performance liquid chromatography HPLC is a method for separating substance mixtures and their qualitative and quantita tive determination The Pump is a conveying system for analytical and semi prepar ative applications Pump head inlays made of different materials cover all needs of the user Stainless steel is pressure re

Smartline Pump 1050 user manual

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