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Tablet Test - Thermo Scientific

1. 44 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 4 Water Analysis Test Menu To determine if a test Is a reverse color test use the Result A B Abs C Abs 2 D Abs equation If the B value is negative the test Is a reverse color test Using the equation Y mX b where m is the slope and b is the y intercept substitute Y with Result and X with Abs Results b m Abs For a reverse color equation the slope is negative and the absorbance color decreases as concentration increases For a standard equation the slope is positive and absorbance color increases as concentration increases How to Modify the Test Method Parameters l 2 3 Press the test key to access the Test Types menu Press the A or Y key to highlight Water Analysis and press the enter key Highlight and change the displayed test parameters including the Wavelength value Ifa reverse color test is being programmed the Reverse Color option must be set to On Water Analysis kihi 6MariS Auto Save Data More parameters Press the A or Y key to highlight More parameters and press the enter key Water Analysis aad 6MariS Result A BA CXA DX3 i 0 0000000 Etor Factor 1 OOoOoo000 C 0 OOo0O000 D 0 OO000000 Result Units mg L Hore parameters Press f or to select item to change a Test Tests Test Press the A or W key to highlight the A B C or D facto
2. Conc Abs 510 0nm Curve Fit Linear Slope 0 531 Intercept 0 037 Std Dev 0 009 Corr Coeff 0 999 Abs Result 510 0nm 128 Orion AquaMate Spectrophotometer User Manual File Edit Format View Help TEST SETUP Orion AquaMate 8000 UV Vis wv1 004 2w R091104 Thermo Scientific Thermo Scientific Chapter 6 Standard Curve Test Menu Editing a Standard Curve You may edit the concentration of any standard on a standard curve In addition you may change the number of standards delete points from the curve or reset the curve data With the standard curve displayed press the A or W key to highlight the standard to be edited and press the Edit Standards function key Standard Curve 10 46am 15ApriS Test Name CHLORINE CURVE BLANK Conc bs 0 126 O 104 0 350 0 216 0 550 0 333 Linear Std Dev 8 S6E 03 Intercept 0 037 Corr Coeff 9 99E 01 Page 1 Standards 1 3 Mos SE al ES Graph Test Standards Test Standard Curve 2 06pm 15AprisS Test Name CHLORINE_CURVE BLANK Conc Edit Concentration Add Standard Delete Standard Clear Measurements Reset Standards Press f or to select Press ENTER Linear Std Dev 9 70E 03 Intercept 0 0146 Corr Coeff 9 98E 01 gt ei Standards 1 3 a If Edit Concentration is selected use the numeric keypad to enter the concentration of the highlighted standard and press the enter key b IfAdd Standard is selected use the numeric keypad to enter the concentrat
3. ENUEN JOST all Thermo Scientific Orion AquaMate UV Vis and Vis Spectrophotometers 269 289800 RevisionC July 2015 Table of Contents ea DCSE Laie areiisestunnsunsveiiauesumininsanci sinanaviniauaveuiveeutandvisnciuivesisnninsinviainiiavasietiavedinegivenaiian 8 Spectrophotometer Introduction sssssssssssssssnsnsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnnnnn 8 Spectrophotometer OvervieW scccssssecsssssssssssrssssersssssssersssssersesssersesssersesesersesasersavesasersaserensases 8 PG UNIS aeieea E A T O 9 Orion AquaMate User Documentation CD s ssesssssresesrsrnsrsnnrnsnnnrnnnnnnrnnnnnnnnnnnrnnrnrnrenenrnres 10 EU a E T E meds eaten nade 10 sale y and Special NOICE aranera E AE 11 CAUCE 2 sinana nn SAE A 12 Spectrophotometer BASICS sssssssssnsusnsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnunnnnnnnnnnnnnnannnnnnnnnnnnnnanananann 12 Spectrophotometer COMPONENS cecssesessssserssssserssssssseesssseersesscersesesersesssersesesersavreeersaveten 12 Instrument CONNECTIONS cccscceessssessssssssssssserssssserersesscrsessceesevsceesevasersasasensevacatensevatensases 13 Instrument Keypad sconces rdnacstrsdavocdiocdaecitrecaetarnanscoe Cones tan aucese taneipactn aipeestanrieseunesanedeeans 13 Vial Holder Carousels saiiresecsaessczestacapa pose rtessntaracae seers heracobitartecpastniees Mociuenansemacrnaeaee ecient 15 Selecting and Positioning Vials and Cuvettes 0 eessseessesersssesesssseressssssesesssseressetteess
4. c b Se A i Initialization of the Vial Holder Carousel When the Run Test function key is pressed to starta measurement the instrument will display the message Calibrating and Checking Turret Please Wait while it attempts to initialize the vial holder carousel Sample Positioner at the blank B position With the AquaMate three position carousel the instrument will rotate the carousel to the default position and then rotate the carousel back to the last selected measurement position B 2 or 4 This ensures that the vial holder is properly positioned in the beam If a one position vial holder carousel has been installed the message Error Single Cell Holder found Use Single Cell Holder will appear Press the Accept Change function key to continue or install the AquaMate three position carousel and press the Cancel Change function key 4 15pm 25Mari5 Error Single Cell Holder Found Use Single Cell Holder So Change Change If the six position vial holder carousel has been installed the instrument will initialize to the blank B position After it has initialized the carousel the instrument will proceed to the test measurement screen Note To prevent a Fatal Error message when removing the vial holder carousel always return to the main menu or the test parameter menu before removing the carousel If the carousel is removed while in the test measurement screen the instrument will display
5. 11 Press the Measure Sample function key to display the result in mg l nitrate as N Notes Some solids may not dissolve Conversion mg l NO mg I N x 4 43 Orion AquaMate Spectrophotometer User Manual 89 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC 2046 Nitrite Tablet Test N 1 Naphthyl ethylenediamine Method 0 01 0 5 mg I N Nitrite as Nitrogen L 2 Pr PY a 8 9 Load and run the AC2046 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one Nitrite LR Tablet straight from the foil into the vial Crush the tablet with a clean Stir rod Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial Wait for a reaction period of 10 minutes Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l nitrite as N Notes The following ions can produce interferences since under the reaction conditions they Cause precipitation antimony Ill iron Ill lead mercury I silver chloroplatinate me
6. Appendix A General Instrument Information Number of Samples Enters the number of samples to be measured in the test Not available in Kinetics or Scanning Number of Standards Enters the number of standards to be measured for the standard curve Printer Selects the output mode as RS 232 or Parallel Utility Protein F actor Enters the factor to calculate protein concentration DNA Bio tests Ref Wavelength Enters a reference wavelength value for each reported measurement measures the analytical wavelength and reference wavelength Reported measurement Abs Analytical WL Abs Reference WL Ref Wavelength Correction Turns reference wavelength correction on or off Run Standard Goes to the standards entry screen Run Test Goes to the data collection screen Sample Positioner Selects the type of Sample Positioner 1 Cell no movement zeros and measures sample in same position Manual 6 vial holder carousel moved by sample positioner keypad always zeros on position B then returns to set position to start measurement Auto 3 vial holder carousel automatically moved to B 2 4 always zeros on position B then goes to position 2 to start measurement Auto 6 vial holder carousel automatically moved to B 1 2 3 4 5 always zeros On position B then goes to position 1 to start measurement Sample Volume Enters the total volume of sample under Dilution Multiplier in some
7. Close the vial tightly with the cap and swirl the vial for 10 15 seconds to mix contents The reagent will not completely dissolve Wipe the exterior of the vial Wait for a reaction period of 2 minutes Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l ortho phosphate Notes Ortho phosphate ions react with the reagent to form an intense blue color Phosphate in organic and condensed inorganic forms meta pyro and polyphosphates must be converted to ortho phosphate ions before analysis Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed inorganic forms Organically combined phosphates are converted to ortho phosphate ions by heating with acid and persulfate The amount of organically combined phosphates can be calculated mg l phosphate organic mg l phosphate total mg l phosphate acid hydrolysable Application for water wastewater and seawater Highly buffered samples or samples with extreme pH values should be adjusted between pH 2 and pH 10 before analysis with 1 mol l hydrochloric acid or 1 mol l sodium hydroxide Phosphate ortho Phosphorus reactive Interferences Large amounts of turbidity may cause inconsistent results Interference Level Interference Level greater than 200 mg l greater than 300 mg l at any level Silica Silicium dioxide greater than 50
8. e Selecting the measurement mode Absorbance or Transmittance e Setting Up Test Parameters e Taking Measurements e Recalling a Test Setting the Parameters for Advanced A T C Method 1 Press the test key to access the Test Types menu 2 Press the Aor VY key to highlight Advanced A T C and press the enter key Test Types 2 12pm 21Apris Water Analysis Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Met Multiwavelength Performance Verification Press T or to select 3 Highlight and change the displayed test parameters including Test Name Measurement Mode Wavelength and Sample Positioner a Some parameters only appear when one of the concentration modes is selected while others appear regardless of the selected measurement mode See the Parameters section for more information on the test parameter settings b Press the A or W key to highlight More parameters and press the enter key to view additional parameters displayed on the second page 4 Press the Save Test function key and follow the onscreen prompts to save the test 144 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements Taking Measurements with the Advanced A T C Method Once the parameters have been set for the absorbance or transmittance test 1 Press the Run Test function key 2 Prepare the blank and samples tha
9. 2 Press the Aor Y key to highlight Advanced A T C and press the enter key Test Types 2 12pm 21AprisS Water Analysis Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Net Multiwavelength Performance Verification Wave length Ref Wavelength Correction Humber of Samples More parameters Press f or to select item to change a ee Se Test Tests Test 4 Press the A or Y key to highlight Load internal test or Load test from USB drive and press the enter Key 5 Press the A or Y key to highlight the desired test and press the enter key 146 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements Concentration Measurements Using the Basic A T C Test Method Use the Basic A T C mode to obtain basic concentration measurements This basic concentration mode is useful for very simple comparisons that do not require a standard curve You cannot save data in this application or factors used when a single standard is measured For better accuracy and the ability to save and recall methods and data use the Standard Curve Test Technique Measuring concentration using the Basic A T C mode is similar to measuring absorbance or transmittance The Basic A T C mode measures concentration using either a standard Conc Std or factor Conc F actor to convert absorbance readings to concentrati
10. _ serverctelnet C Mark 6 bits 127 0 0 1 telnet Winsock is DSR 6 C Space 5 bits 127 0 0 1 9876 C Raw Ring 3 double click to scan ports Telnet BREAK Error Char Count 0 CPS 0 Port 3 57600 8N1 None Click the Baud dropdown menu and select 19200 m RealTerm Serial Capture Program 2 0 0 70 lft Display Port Capture Pins Send EchoPort 120 12C 2 12CMisc Misc n Clear Freeze Status Baud aB v Port 9 Serial2 v Open Spy f Change u Connected 57600 al Software Flow Control SDE Pari 38400 a Sae Receive Xon Char 17 TXD 3 cee _Bbits 1bit C 2bits l E CTS 8 A f bits Hardware Flow Control Transmit Xoff Char 19 DCD 1 C 1300 bits None C RTS CTS ee DSR 6 C 921600 bits C DTR DSR C RS485 rts C Raw Ring 460800 7 Telnet BREAK Error Char Count 0 CPS 0 Port 3 57600 8N1 None 192 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific 6 Click the Change button to save the port settings Chapter 11 Customer Services tm RealTerm Serial Capture Program 2 0 0 70 Display Port Capture Pins Send EchoPort l2c 12C 2 12CMisc Misc Baud 19200 y Port v Open Spy Parity Data Bits gt Stop Bits o wun 17 None bits 1 bit C 2bits f sept C bits Hardware Flow Control Transmit Xoff Char 19 Pee Ghits None C RTS C
11. e Taking Measurements e Recalling a Test Setting the Parameters for Absorbance Ratio Method 1 Press the test key to access the Test Types menu 2 Press the A or W key to highlight Absorbance Ratio and press the enter key Test Types Water Analysis Advanced A xT C Standard Curve Absorbance Eatio Absorbance Difference Kinetics Scanning 3 Point Met Multiwavelength Performance Verification ee Start Tests ATC 3 Highlight and change the displayed test parameters including Test Name Wavelength 1 Wavelength 2 and Sample Positioner See the Parameters section for more information on the test parameter settings Absorbance Ratio Test Name Wavelength 1 Wavelength 2 Ref Wavelength Correction sample Positioner Number of Samples More parameters Press f or to select item to change ee Se Test Tests Test 4 Press the Save Test function key and follow the onscreen prompts to save the test Orion AquaMate Spectrophotometer User Manual 157 Chapter 8 Absorbance Transmittance and Concentration Measurements Taking Measurements with Absorbance Ratio Method Once the parameters have been set for the absorbance ratio test 1 Press the Run Test function key 2 Prepare the blank and samples that will be measured 3 If Auto 6 or Auto 3 is selected for the Sample Positioner setting a Place the blank and samples in the appropriate positions on the carousel b Confirm that the blank and sam
12. 100 tests AC2016 Sulfide DPD catalyst method tablet reagent 50 tests AC2065 Zinc zincon method tablet reagent 50 tests Visit www thermoscientific com water for a complete listing of all available Thermo Scientific Orion meters electrodes solutions and accessories Thermo Scientific Orion AquaMate Spectrophotometer User Manual 201 General Instrument Information Parameters X Enters math operators when in the calculator mode Utility of Lamp Life Used we a i aa of lamp life used based on a typical Xenon 3 Pt Net Calculates peak height from the tangential baseline in the graph Scanning Absorbance Enters the absorbance value Accept Name Accepts the displayed name entry Test Name and Edit Units Add Character Adds a highlighted character to the name entry Test Name and Edit Units dan Adds a wavelength and factor to the list in multi wavelength tests and some performance verification tests Area Calculates area under the peak in the graph Scanning AutoP rint Turns the automatic printout on or off Autoscale Rescales the graph to the original ranges of X and Y axes Kinetics Scanning Baseline Expiration Enters time when the baseline for scan tests needs to be collected again Utility Beeper Turns the audible signal for key presses on and off Utility Calculation Baseline Selects the zero baseline or the tangential baseline to calculate area under the pe
13. Fatal Error 8 Press Esc to return to main menu If this message Is displayed press the esc key and then press the Run Test function key to re initialize the vial holder carousel Orion AquaMate Spectrophotometer User Manual 19 Orion AquaMate Instrument Setup Entering Parameter Values The following instructions outline the use of the Orion AquaMate instrument keypad with various menus settings and controls Numeric Entry Highlight the desired parameter i e Wavelength and start typing the numeric value The Entry window will open with the range of the parameter shown below Type the desired value and then press the enter key Number of Samples More parameters Entry Enter Wavelength 190 0 1100 0 ee T ee ee 20 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 3 Orion AquaMate Instrument Setup Menu Selection Highlight the desired parameter i e Units and press the enter key to display the selection list Press the A or V key to highlight the appropriate item on the list and press the enter key Advanced A xT C 1 15pm 25Mari5 Humber of Samp More paraneter Press f or to select Press ENTER Press f or 4 t On Off Toggle Highlight the desired parameter and press the enter key to toggle the parameter On or Off Advanced A xT C Delay Time min sec Conc of Standard Factor Units sample Positioner Humber of Samples More para
14. Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2095 WA Phosphate Ortho Low Range Tablet Test Phosphomolybdic Acid Ascorbic Acid 0 05 4 mg l PO L 2 Pr PY a 9 Load and run the AC 2095 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one Phosphate No 1 LR Tablet straight from the foil into the vial Crush the tablet with a Clean stir rod Add one Phosphate No 2 LR Tablet straight from the foil into the vial Crush the tablet with a Clean stir rod Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial Wait for a reaction period of 10 minutes 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l ortho phosphate Notes Only ortho phosphate ions react The tablets must be added in the correct sequence The test Sample should have a pH value between 6 and 7 Interferences Higher concentrations of Cu Ni Cr Ill V V and W VI interfere due to their color Silicates do not interfere masked by citri
15. Press the Set Options function key to access the set options window Press the A or Y key to highlight Factor use the numeric keypad to enter the desired factor value and press the enter key Press the A or Y key to highlight Calculation baseline and press the enter key to toggle between the Zero and Tangent settings Press the esc key once all settings are complete The instrument will calculate the area under a curve value for the selected wavelengths factor and calculation method Scanning 41pm 16AprisS Test Name SCAN_O1 464 517 0 2355 0 350 0 370 Wave length 550 0 Press the Peaks amp Valleys function key to label the valleys and peaks on the graph Press the A or Y key to highlight the type of labels to display and press the enter key The instrument will label up to nine peaks or valleys on the scanned data graph 0 9090 2 24pm 16Apris SCAN_O1 BLANK Label Peaks OFf Label V Valleys OFF Features 4 Press f or to select Press ENTER to change Press ESC to exit T T TO T T T oT NT OSSO 450 0 Wave length 550 0 IDH 2 Se col lected T Orion AquaMate Spectrophotometer User Manual 139 Chapter 7 Wavelength Scanning Test Menu Rescaling Tabular Scan Data Modify the tabular scan data by performing calculations on the data 1 Inthe tabular display press the Edit Data function key 2 Press the A or V key to highlight Select Data and press the enter key Scanning 2 59pm 16AprisS Test N
16. Reverse color methods use a reagent that when prepared with samples deceases in color as the concentration of the species being measured in the samples increases Reverse color methods require the use of both a blank and a reagent blank The blank is a clear solution deionized water with zero absorbance The reagent blank is a mixture of the reagent and deionized water or initial reagent and sample as in the zinc by zincon method and provides a zero concentration point with the darkest color highest absorbance The color of samples prepared with the reagent will decrease as the concentration increases The following provides an overview how to perform a reverse color method 1 Load the test method in the Water Analysis test menu The Reverse Color option should be set to On for the method Water Analysis 10 06am 4 7MariS BLANK Test Name ACZOOD Measurement Mode Absorbance Wave length 580 Onm ipt Adjust orf Reverse Color On sample Positioner Manual IDH O 0FF Statistics Auto Print Auto Save Data More parameters Press f or to select item to change es ee Test Tests Test 2 Press the Run Test function key 3 Filla vial with deionized water Close the vial tightly with the cap Wipe the exterior of the vial 4 Place the vial into the holder in the sample chamber and close the chamber door 5 Press the Measure Blank function key to measure the blank Water Analysis 10 09am 4 7MariS Test Mame ACZOO9 BL
17. Waitfora reaction period of 3 minutes 9 Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l iron Notes Thermo Scientific The reagent reacts with all soluble iron and most insoluble forms of iron in the water sample Iron oxide requires prior digestion use mild vigorous or Digesdahl digestion An example of digestion with acid is described here Add 1 ml of concentrated sulfuric acid to 100 ml water sample Heat and boil for 10 minutes or until all particles are dissolved After cooling down the sample is set to a pH value of 3 to 6 by using ammonia solution Refill with deionized water to the previous volume of 100 ml and mix well 10 ml of this pre treated solution is used for analysis perform as described by the selected test method ll Water that has been treated with organic compounds like corrosion inhibitors must be oxidized as necessary to break down the Iron Therefore add 1 ml concentrated sulfuric acid and 1 ml concentrated nitric acid to 100 ml water sample and boil to approximately half volume After cooling down proceed as described above Very strong alkaline or acidic Samples must be adjusted to a pH value between 3 and 5 before analysis Accuracy is not affected by undissolved powder Water samples containing visible rust should be allowed to react for at least 5 minutes Orion AquaMate Spectrophoto
18. the concentration absorbance and calculated factor will be displayed Advanced A xT C 1 lipm 8Ju115 Test Name BLANK A Factor 510 0nm calculated Page 1 Standard 1 aS ES Le Test Standard Test 7 To save the test with the calculated factor press the Save Test function Key and follow the onscreen prompts 8 Press the Run Test function key to start measuring samples Taking Measurements with Advanced A T C Method Once the parameters have been set for the concentration test 1 Press the Run Test function key 2 Prepare the blank and samples that will be measured 3 If Auto 6 or Auto 3 is selected for the Sample Positioner setting a Place the blank and samples in the appropriate positions on the carousel b Confirm that the blank and samples are in the appropriate positions on the carousel and press the enter key c Once the blank and samples have been measured remove them from the carousel d To measure additional samples press the Measure Sample function key and repeat Steps 3a through 3c 4 If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting a Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank has been measured remove it from the carousel 152 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements b Place the sample in th
19. 000 1 200 2590 0 10 000 Place Standards 1 2 in positions i1 2 to bs Std otd Test 4 To switch between absorbance and Transmittance press the Change to T or Change to Abs function key until the appropriate mode Is displayed 5 You will need to set three values for each standard the wavelength the absorbance or Transmittance and the tolerance value To add a standard a Press the Add Std function key use the numeric keypad to enter the wavelength value in the entry field and press the enter key b Use the numeric keypad to enter the absorbance or T value for the entered wavelength in the entry field and press the enter key Orion AquaMate Spectrophotometer User Manual 173 Chapter 9 Performance Verification c Use the numeric keypad to enter the tolerance value for the entered wavelength in the entry field and press the enter key d Repeatfor each standard 6 Verify that the wavelengths absorbance or T and tolerances are set correctly 7 Press the Start Test function key 8 The results will indicate pass or fail for each wavelength lf the test fails follow these guidelines e Repeat the test twice to verify that the test is failing consistently e Follow the guidelines provided with the standard reference materials e Contact technical support for more troubleshooting advice 174 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 9 Perfor
20. 1 mg l Fe 1 Load and run the AC207824 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Iron LR Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 7 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 8 Waitfora reaction period of 5 minutes 9 Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l iron Notes Alternatively the AC207850 method can be used with 50mm rectangular vials All blank and sample volumes must remain the same as those specified in these instructions so Samples may need to be prepared in separate containers and then transferred into the selected vial This method determines the total dissolved iron as Fe2 and F e For the determination of total dissolved and undissolved tron Soluble and insoluble iron digestion is required An example is described here Add 1 ml of concentrated sulfuric acid to 100 ml water sample Heat and boil for 10 minutes or until all part
21. 10 ml of sample Add one Glycine Tablet straight from the foil into the vial Crush the tablet with a clean stir rod Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Transfer the contents of the second vial into the first vial Orion AquaMate Spectrophotometer User Manual 69 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate 10 Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial 11 Place the vial into the holder in the sample chamber and close the chamber door 12 Press the Measure Sample function key to display the result in mg l chlorine dioxide Notes Alternatively the AC209950 method can be used with 50mm rectangular vials All blank and sample volumes must remain the same as those specified in these instructions so Samples may need to be prepared in separate containers and then transferred into the selected vial Vial cleaning As many household cleaners i e dishwasher detergent contain reducing Substances the subsequent determination of chlorine dioxide may show lower results To avoid any measurement errors only use glassware free of chlorine demand Preparation Put all applicable glassware into sodium hypochlorite solution 0 1 g l for one hour and then rinse all glassware thoroughly with deionized water Preparing the sample When preparing the sampl
22. ATC 3 Highlight and change the displayed test parameters including Test Name Wavelength 1 Wavelength 2 Wavelength 3 Factor Units and Sample Positioner See the Parameters section for more information on the test parameter settings 3 Point Net Test Name sample Positioner Humber of Samples More parameters Press f or to select item to change ee Se Test Tests Test 4 Press the Save Test function key and follow the onscreen prompts to save the test Orion AquaMate Spectrophotometer User Manual 161 Chapter 8 Absorbance Transmittance and Concentration Measurements Taking Measurements with 3 Point Net Method Once the parameters have been set for the 3 point net test 1 Press the Run Test function key 2 Prepare the blank and samples that will be measured 3 If Auto 6 or Auto 3 is selected for the Sample Positioner setting a Place the blank and samples in the appropriate positions on the carousel b Confirm that the blank and samples are in the appropriate positions on the carousel and press the enter key c Once the blank and samples have been measured remove them from the carousel d To measure additional samples press the Measure Sample function key and repeat Steps 3a through 3c 4 If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting a Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank ha
23. ATC 3 Press the A or V key to highlight Measurement Mode and press the enter key Press the A or Y key to highlight Conc Std or Conc F actor and press the enter key Advanced A z IT C Test Name Absorbance Transmittance Wave length Ref Wavelengt Conc Factor Delay Time mi sample Positio Press fT or to select Press ENTER More paraneter Press f or to select item to change 4 Highlight and change the displayed test parameters including Test Name Wavelength Concentration of Standard Factor Units and Sample Positioner 150 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements a Some parameters only appear when one of the concentration modes is selected while others appear regardless of the selected measurement mode See the Parameters section for more information on the test parameter settings b Press the A or W key to highlight More parameters and press the enter key to view additional parameters displayed on the second page Advanced A xT C anaes 6 Ju115 Test Name Measurement Mode ave length Undefined Undefined C sample Positioner Manual 6 More parameters Press f or to select item to change Pa Standard Test Tests Advanced A xT C aaa st 6 Ju115 IDH O 0FF Low High Limits Statistics Auto Print Auto Save Data More parameters Press f or to select item to change na
24. Adjust Factor Reverse Color Auto Save Data More parameters Press f or to select item to change ipt Adjust Test Tests Test 34 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 4 Water Analysis Test Menu 7 Press the A or key to highlight Load test from USB drive and press the enter key Water Analysis 11 05am 440cti2 BLANK Load internal test Load test from USE drive Press f or to select Press ENTER Press ESC to exit Auto Save Data More parameters Press f or to select item to a Utility Files Directory 11 05am 240ctiz AC4P12 WA 6Juni2 AC4SP71 WA AC4P 72 WA CODLOOG WA More tests Press fT or to select test I i Oo est O Tet 9 Press the Save Test function key Water Analysis 11 06am 440cti2 BLANK Test Name CODLOO Saved Measurement Mode Wave length ipt Adjust Reverse Color Data File Name More parameters Press f or to select item to change S TA ET Test Tests Test 10 Press the A or Y key to highlight Save as an internal test and press the enter key Thermo Scientific Orion AquaMate Spectrophotometer User Manual 35 Chapter 4 Water Analysis Test Menu 11 12 13 Water Analysis 11 07am 440cti2 BLANK Save as an internal test Save test to the USE drive Save test to internal and USB Press f or to select Press ENTER Press ESC to exit Data File Name More parameters Press f or to
25. Bio Tests Save Test Saves all parameters of the current test in internal memory for later recall Scan Speed Selects the speed nm min for a scan as Slow Medium or Fast Scanning Screen Contrast Improves visibility of the display by changing the contrast between the background and text Utility Select Test Tags the highlighted test name with gt to include the test in the SmartStart menu Utility Stored Tests Directory Set Max X Set Min X Sets the cursor position in the graph as the minimum X and the maximum X values for recalculating rate Kinetics Setnms Use to enter and edit the wavelength and factor values Set Options Selects the factor entry or the baseline to calculate area under the peak in the graph Scanning Setup Correction Initiates the procedure to collect the data necessary to correct for absorbance differences between vials or cuvettes Slope Enters abs concentration value Standard Curve Smoothing Turns data smoothing on and off Scanning Software Revision Displays the version of firmware in the instrument Utility SRE Tolerance Acceptable minimum stray light Standard Concentrations Enters the concentration of standards used to generate the standard curve for the test Standby Selects the time since the last keystroke or instrument activity powers down the unit to save lamp life Utility Start Wavele
26. Instructions for Orion AquaMate Preparation Put all applicable glassware into sodium hypochlorite solution 0 1 g l for one hour and then rinse all glassware thoroughly with deionized water For individual testing of free and total chlorine the use of different sets of glassware Is recommended EN ISO 7393 2 5 3 Preparing the sample When preparing the sample the escape of chlorine gases i e by pipetting or shaking must be avoided The analysis must take place immediately after taking the sample The DPD color development is carried out at a pH value of 6 2 to 6 5 The reagents therefore contain a buffer for the pH adjustment Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the reagent is added use 0 5 mol l sulfuric acid or 1 mol l sodium hydroxide Exceeding the measuring range Concentrations above 10 mg l chlorine using tablets can lead to results showing 0 mg l In this event the water sample must be diluted with water free of chlorine 10 ml of the diluted sample should be mixed with the reagent and the measurement repeated Turbidity can lead to errors The use of the DPD No 1 tablet in samples with high calcium ion contents and or high conductivity can lead to turbidity of the sample and therefore incorrect measurements Oxidizing agents such as bromine or ozone interfere as they react in the same way as chlorine Orion AquaMate Spectrophotometer User Manual 61 Chapter 5 O
27. Linear Regression general case A A c Where A absorbance c concentration A c is defined by an equation of the form A c aac4 a3C3 a2c ac ao Where ao Y axis intercept a1 a4 Coefficients Coefficients are computed using the least squares method Linear regression through zero A ai c Where A absorbance c concentration ai slope The slope is calculated as a1 SXY SXX This model requires Slope is not equal to zero or infinity Atleast one standard data point with concentration gt 0 The absorbance of the 0 concentration blank 0A Thermo Scientific Orion AquaMate Spectrophotometer User Manual 209 Appendix A General Instrument Information Calculation Calculation Equation Segmented model The segmented model requires Data for at least two standard data points with different concentration and absorbance Slopes of all segments must be ascending positive or descending negative Validity of standard curves A c1 gt A c2 for all c1 gt cz or A c1 lt A c2 for all c1 gt c2 Where A absorbance C1 C2 concentration Graph of Valid Nonlinear Standard Curve T lt GG0RBANCE MPASLINED w ABSORBANCE wm z IHECHTATOH E If this is not the case there will be more than one solution within the specified domain and the message Curve cannot be used to determine sample concentrations it may produce
28. Sodium hypochlorite solution 0 1 g l for one hour and then rinse all glassware thoroughly with deionized water Preparing the sample When preparing the sample the lost of ozone i e by pipetting or Shaking must be avoided The analysis must take place immediately after taking the sample The DPD color development is carried out at a pH value of 6 2 to 6 5 The reagent tablet therefore contains a buffer for the pH adjustment Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the tablet is added use 0 5 mol l sulfuric acid or 1 mol l sodium hydroxide Exceeding the measuring range Concentrations above 6 mg l ozone can lead to results showing 0 mg l In this case the water sample must be diluted with water free of ozone 10 ml of the diluted sample should be mixed with the reagent and the measurement repeated Oxidizing agents such as bromine and chlorine interfere as they react in the same way as ozone Orion AquaMate Spectrophotometer User Manual 97 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2001 pH Tablet Test Phenol Red Method 6 5 8 4 pH 1 Load and run the AC2001 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to meas
29. Test Loading Saved Test Files All saved test files can be loaded from the internal instrument memory or USB memory stick using the main Utility menu 1 Press the utility key to access the Utility menu 2 Press the A or W key to highlight Stored Tests Directory and press the enter key 3 Select the location of the saved test files to be loaded a To load saved test files from the internal memory press the A or W key to highlight Load internal test and press the enter key b To load saved test files from the USB memory stick press the A or Y key to highlight Load test trom USB drive and press the enter key 4 Press the A or Y key to highlight the desired test and press the Load Test function key 5 The test will be loaded in the corresponding test technique menu Note Saved test files on the USB memory stick can be viewed or deleted by selecting the USB Drive Files option in the Utility menu Alternatively saved test files can also be loaded using their specific test technique menu 1 Press the test key to open the Test Types menu 2 Press the A or Y key to highlight the desired test technique and press the enter Key 3 Press the Stored Tests function key 4 Press the A or Y key to highlight Load internal test or Load test from USB drive as the test location and press the enter key 5 Press the A or Y key to highlight the desired test and press the Load Test function key 6 The test will be loaded in the test
30. The results can be saved to the USB stick if desired If the reading is gt 0 900 absorbance dilute and retest Multiply the reading by the dilution factor If the results are lt 0 010 absorbance consider using a larger cell Load the appropriate method and rezero the instrument Quality Control QC Run an SCS and duplicate samples with each batch or run QC samples per your QA plan For SUVA testing follow requirements of EPA Method 415 3 See Reference 1 pH Adjustment of Sample 1 Note Do not adjust the pH of a sample which will be used for a SUVA calculation Proceed to the filtration step Calibrate the pH probe in pH 4 01 and 7 00 buffers Warm the sample up to room temperature Shake the sample to insure homogeneity Measure 50 mL of the sample to a 100 mL beaker using a graduated cylinder Immerse the pH probe in the sample and record the initial pH Adjust the sample into the range of pH 4 to 10 by adding drop wise 0 1N sodium hydroxide to raise the pH or by adding drop wise 0 1N hydrochloric acid to lower the pH Different Strength acid or base can be used if needed 8 Note that the overall volume change should not be greater than 1 0 5 mL Discard and re prepare with stronger acid or base if the volume changes more than 1 9 Record the adjusted pH Proceed to the filtration step p 1 a oe a YS i Results of SCS Testing on the Orion AQ8000 Spectrophotometer 25 0 mg L organic carbon KHP Bias Exp
31. Utility Stored Tests Directory Wavelength Enters values for the analytical wavelengths 206 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Appendix A General Instrument Information Concentration Units This section covers how to specify concentration units and create custom units To Specifying Concentration Units Concentration and Kinetics tests include a parameter for units which labels the results The units parameter offers a list of common concentration units and each test method uses this list e C concentration e M L e ppm e mM L e ppb e U e g L e pM uL e mg L e ng uL e ug L 1 Inthe test method press the A or Y key to highlight Units and press the enter key 2 Press the A or Y key to highlight the desired concentration units and press the enter key Advanced A xT C 11 36am 41Apris Test Name Measurement Mo Wave length Ref Wavelengt Humber of Samp More paraneter Press f or to select Press ENTER Press fT or 4 t nity Unit To Create Custom Units In addition to the common concentration units you can create a custom concentration unit and add it to the list This custom concentration unit can be changed when desired Only one custom concentration unit is available in the list at any given time 1 Inthe test method press the A or Y key to highlight Units and press the enter key 2 With the list of units shown press the Edit Units function k
32. ambiguous results will appear when the curve is viewed Graph of Invalid Nonlinear Standard Curve A ABO ANI MEASURED ABSORBANCE B 210 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Statistics Linear regression general case Appendix A General Instrument Information Calculation Calculation Equation Where N Degree of polynomial ISSXY SOX SOY The calculation for the correlation coefficient applies only to first order linear regression curves first degree polynomials Linear regression through zero model ISYY a SXY N 1 Absa Abs AbSref Absorbance Ratio AbsAy er ee Result AbsA factor AbsAz factor2 Absorbance Difference or Result AbsA1 AbsAref factor AbsA2 AbS ret factorz 3 Point Net Baseline correction absorbance Az Az Az 4 1a A2 ee i 3 Point Net Absorbance Sample Curve ABSORBANCE WAVELENGTH 3 Point Net ASTM E16904 Baseline correction absorbance Az A 4 1a A3 7 A ABSORBANCE WAVELENGTH Thermo Scientific Orion AquaMate Spectrophotometer User Manual 211 thermoscientific com water 2015 Thermo Fisher Scientific Inc All rights reserved Microsoft and Windows are registered trademarks of Microsoft Corporation All other trademarks are the property of Thermo Fisher Scientific Inc amp its subsidia
33. ass hp eves E a A acre nero 182 Replacing Ine TUNOSEN LAMP erraien 184 Chapter L iymoniinon 186 CUSTOMER Services annsna ene a ulanmar area ainE 186 Technical SUPPO creion a a nrcshsnea auld ciahned 186 External Device Interfacing and Data Transfer s ssssssrsrsrsrnrnrnnrsrsrnrnrnnnnrnrnrnrnnrnrernrrnrnnrnrnrnrnns 187 AquaMate Spectrophotometer Setup for Computer Interfacing s e 187 USB Computer Cable Interfacing and USB Driver Installation ereenn 188 Example Data CONC CUON Progra neninn nan 191 AquaMate Spectrophotometer Setup for Printer Interfacing 195 USB Printer Cable Interfacing and Printing Data cccscssesssssssssssssesssessteess 196 MS TUMERE S PECIICATIONS ciusnrsscasmonsperenncn poa TEAR 197 POSING ITOH ATOM ice sataed ova scetaed duaba dts tendhietnatianecusiedanataturtinch te waterside ei Abaulsecibacdeaaicausiedds 199 PODGNGIN A crencas 202 General Instrument Information ssssssssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnunnnnnnnnnnnnnnnnnnnannnnnananan 202 P arame tE ene S N O A 202 CONCEMEATON UTIINS aa tna macubelnaamenne 207 CAICUIAU ONS TOL SOW all vce visiosnasias sett scaisnsia veers thie a A tans A NR 209 Spectrophotometer Introduction Spectrophotometer Overview Thermo Scientific Orion AquaMate UV Vis and Vis spectrophotometers offer the following features and benefits Easy operation using the 260 preprogrammed methods for common colorimetric reagents Flexibility to create new methods for additio
34. ccssseesseserssserssssreesssererssserersesererseses 30 DEEG TS E E thas elas E E 30 Using the SmartStart Test FCAT Cs sicininstisransncvcvnssenvensedsiciendarseivu tincviesiinkelsarcaudogersaiviveds 30 Chapter A AEE E EE E A E E 32 Water Analysis Test Menu cicsisisisdssiverivsracieiaushcatsniiniviavivagninnsdpinanmainiapeapiaiuisiintancan 32 Preprogrammed MethodS s ssssssssresrsrnresnsrnrnnnnrnrnnnnnnnnnnnrknnNrnNENrErENENNENENENNENENENEE NEEE rEnEEn rnrn nnne na 32 Loading and Running a Water Analysis Method on the AquaMate Instrument 33 Loading Test Methods from USB Stick to AquaMate Instrument 34 Running a ESE Method cs cisedscatrapersavaiaabanstucthidndiusteisiideudsvetseu ined vans stauseardiwetaedinnag daiuetis 37 Running a Water Analysis Test Method s ssssssrsrsrsrsrnrnununnrnnnrnrnrnnnnrnnnrnnrnnnrnrnrnrnrnnrernrernns 38 Using the One Point Adjustment Feature cassis ccscncnnhsancintannnnsteranutitartshanesionannndaincesinsauiianisnwiswenn 40 Using the Reverse Color Feature ssissnnsnersnwaiteesnrvneunnsieenenisiinsnitalnes vinbieniicdugnitnescnntienitnngits ahuniptlies 42 Creating or Modifying a Test M thOd ccsscssscssssesssscssscssseessssessserssserssversssersssersesereasereesenen 44 How to Define the Test Method Parameters ccccssesssserssssersssssersssssrersssrersesrerseses 44 How to Modify the Test Method Parameters ccccsssssssssssssssesssesesssessesssesrseeasen 45 Chapter Saomioomiap an vavasisauisssuuasvdaseii
35. contents while still warm Allow the vials to cool to room temperature before measuring Wipe the exteriors of the vials 6 Load and run the CODHPO method 7 Place the blank vial into the holder in the sample chamber and close the chamber door 8 Press the Measure Blank function key to measure the blank 9 Open the sample chamber door Remove the blank vial from the holder 10 Place the sample vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l oxygen Notes Run samples and blanks with the same batch of vials The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch Do not place the hot vials in the sample chamber Cool the vials to room temperature for final measurements Suspended solids in the vial lead to incorrect measurements For this reason It Is important to place the vials carefully in the sample chamber The precipitate at the bottom of the sample should be not suspended Clean the outside of the vials with a towel Finger prints or other marks must be removed Samples can be measured when the chloride content does not exceed 1000 mg l In exceptional cases compounds contained in the water cannot be oxidized adequately so results may be lower than reference methods For samples under 1000 mg l it is recommended to repeat the test using the COD mid range test CODH00 or
36. evaluate the quality of a material This test technique is useful when using colorimetric reagents for which the test kit manufacturer does not specify a wavelength parameter for the test kit Always follow the reagent instructions provided by the test kit manufacturer when creating a custom method Note If the manufacturer does not specify the factor or equation for obtaining the concentration of the test kit the Standard Curve test technique should be used with prepared standards to determine the factor or equation for the reagent Use Scanning Test Technique e Toscan samples by setting parameters collecting a baseline scan and scanning a sample e To view and manipulate scanning data including rescaling graphical scan data determining peak height using a 3 point net equation calculating the area under a curve and labeling peaks and valleys e To recall existing scanning methods 132 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 7 Wavelength Scanning Test Menu Setting the Parameters for a Scan 1 Press the test key to access the Test Types menu 2 Press the A or key to highlight Scanning and press the enter key Test Types 3 53pm 15Apris Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics a 3 Point et Multiwavelength Performance Verification Press T or to select stare Teste arc 3 Highlight and change the dis
37. final measurements Suspended solids in the vial lead to incorrect measurements For this reason It Is important to place the vials carefully in the sample chamber The precipitate at the bottom of the sample should be not suspended Clean the outside of the vials with a towel Fingerprints or other marks must be removed Samples can be measured when the chloride content does not exceed 1000 mg l In exceptional cases compounds contained in the water cannot be oxidized adequately so results may be lower than reference methods For samples under 100 mg l itis recommended to repeat the test using the COD low range test CODLO0 Orion AquaMate Spectrophotometer User Manual 73 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate CODHPO COD High Range Digestion Tube Test Dichromate Digestion Method 0 15000 mg l 02 High Range 1 Open one 16mm COD reaction vial and add 0 2 ml of deionized water this is the blank vial 2 Opena second 16 mm COD reaction vial and add 0 2 ml of sample this is the sample vial 3 Close the vials tightly with the caps and gently invert the vials several times to mix the contents CAUTION The vials will become hot during mixing 4 Heatthe vials for 120 minutes in the preheated reactor at a temperature of 150 C 5 CAUTION The vials will be hot Remove the vials from the reactor and allow them to cool to 60 C or less Gently invert the vials several times to mix the
38. for samples under 100 mg l it is recommended to repeat the test using the COD low range test CODL00 74 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2029 Copper Free amp Total Tablet Test Biquinoline Method 0 05 5 mg l Cu 1 Load and run the AC202924 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Copper No 1 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 7 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 8 Place the vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Sample function key to display the result in mg l free copper 10 Open the sample chamber door and remove the vial from the holder 11 Add one Copper No 2 Tablet straight from the foil into the same vial Crush the tablet with a Clean stir rod 12 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the
39. keypad to enter the concentration of the standard and press the enter key to save the value 124 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 6 Standard Curve Test Menu Standard Curve 10 54am 15ApriS Test Name CHLORINE CURVE BLANK Conc AL 510 0nm b Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank has been measured remove it from the carousel Standard Curve 10 37am 15ApriS Test Name CHLORINE CURVE BLANK Conc bs 510 0nm c Place the standard in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Standard function key and press the enter key to measure the standard Repeat for each standard Standard Curve 10 43am 15ApriS Test Name CHLORINE CURVE BLANK Conc bs 510 0nm Page 1 Standards 1 2 Sie Es tia Sans Blank Test Standards Standard 5 When all of the standards have been measured the absorbance of each standard will be displayed with the slope intercept and correlation coefficient of the standard curve Thermo Scientific Orion AquaMate Spectrophotometer User Manual 125 Chapter 6 Standard Curve Test Menu Standard Curve 10 46am 15ApriS Test Name CHLORINE CURVE BLANK Conc bs 510 0nm 0 120 0 104 0 350 0 216 0 550 0 333 Curve Fit Linear 5 lope 0 531 Std Dev 8 S6E 03 Interc
40. mol l nitric acid or 1 mol l sodium hydroxide Concentrations above 10 mg l Cu causes too high test values if the reaction time of 5 minutes is increased so itis very important to perform the test procedure as described Substances which may interfere when present in concentrations at sO mal 1000 mi 50 mal s0 rg NI Level 88 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ACRO007 Nitrate Reaction Tube Test Chromotropic Acid Method 1 30 mg I N Nitrate as Nitrogen L 2 6 1 8 9 Load and run the ACR007 method Open one 16 mm reaction vial Reagent A and add 1 ml of deionized water this is the blank vial Open a second 16 mm reaction vial Reagent A and add 1 ml of sample this is the Sample vial Add the contents of one Nitrate Chromotropic Powder Pack straight from the foil into each vial Close the vials tightly with the caps and invert gently about 10 times to mix contents Some Solids may not dissolve Wipe the exteriors of the vials Wait for a reaction period of 5 minutes Place the blank vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door Remove the blank vial from the holder 10 Place the sample vial into the holder in the sample chamber and close the chamber door
41. of the diluted sample should be mixed with the reagent and the measurement repeated Oxidizing agents such as bromine or ozone interfere as they react in the same way as chlorine 66 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P72 Chlorine Total Powder Pack Test DPD Method 0 02 2 mg l Cl 1 Load and run the AC4P 72 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add the contents of one Chlorine Total DPD F10 Powder Pack straight from the foil into the vial 7 Close the vial tightly with the cap and swirl or invert several times to mix the contents approximately 20 seconds Wipe the exterior of the vial 8 Waitfora reaction period of 3 minutes 9 Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l total chlorine Notes Vial cleaning As many household cleaners i e dishwasher detergent contain reducing Substances the subsequent determination of chlorine may show lower result
42. period of 5 minutes 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l manganese Orion AquaMate Spectrophotometer User Manual 85 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P54 Manganese Low Range Powder Pack amp Liquid Test PAN Method 0 01 0 7 mg l Mn 1 Load and run the AC4P54 method 2 Use two clean AQUAfast 24mm round vials Cat No AC2V24 3 Pour 10 ml of deionized water into the first vial this is the blank vial 4 Pour 10 ml of sample into the second vial this is the sample vial 5 Add the contents of one Ascorbic Acid Powder Pack straight from the foil into each vial Close the vials tightly with the caps and swirl or invert several times to mix the contents 6 Add 15 drops of Alkaline Cyanide Reagent Solution to each vial Add drops of the same size by holding the bottle vertically and squeezing slowly Close the vials tightly with the Caps and swirl or invert several times to mix the contents 7 Add 21 drops of PAN Indicator Solution to each vial Add drops of the same size by holding the bottle vertically and squeezing slowly Close the vials tightly with the caps and swirl or invert Several times to mix the contents Wipe the exteriors of the vials 8 Waitfora reaction period of 2 minutes 9 Place the blank vial into the holder in the sample chamber and close th
43. reducing substances interfere o Cupric and ferrous ions cause low results o Antimonous auric bismuth chloroplatinate ferric lead mercurous metavanadate and silver ions interfere by causing precipitation o In samples with very high concentrations of nitrate gt 100 mg l N a small amount of nitrite will be found Such high levels of nitrate appear to undergo a slight amount of reduction to nitrite either spontaneously or during the reaction time of the test Orion AquaMate Spectrophotometer User Manual 91 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ACD004 Nitrogen Total Low Range Digestion Tube Test Persulfate Digestion Method 0 5 25 mg l N Nitrite as Nitrogen l 10 11 12 l3 14 15 16 17 18 Open two TN Hydroxide LR Digestion Vials and add the contents of one TN Persulfate Reagent Power Pack straight from the foil into each vial Use a funnel to add the reagent Wipe off any persulfate reagent that may get on the lid or tube threads Add 2 ml of deionized water to the first digestion vial this is the blank vial Add 2 ml of sample to the second digestion vial this is the sample vial Close the vials tightly with the caps and shake the vials for at least 30 seconds to mix the contents The reagent many not dissolve completely Heat the digestion vials for 30 minutes in the preheated reactor at a temperature of 100 C CAUTION The vials will be hot R
44. samples with high calcium ion contents and or high conductivity can lead to turbidity of the sample and therefore incorrect measurements e Oxidizing agents such as bromine or ozone interfere as they react in the same way as chlorine Thermo Scientific Orion AquaMate Spectrophotometer User Manual 63 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2072 Chlorine Total Tablet Test DPD Method 0 01 6 mg l Cl 1 Load and run the AC207224 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Empty the vial leaving a few drops of sample remaining in the vial 7 Add one DPD No 4 Tablet or one DPD No 1 Tablet and one DPD No 3 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 8 Add sample to the 10 ml mark on the vial 9 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 10 Wait for a reaction period of 2 minutes 11 Place the vial into the holder in the sample chamber and close the chamber door 12 Press the Measure Sample function key to display the result
45. select item to Press the Accept Name function key Create Test Mame 11 07am 240cti2 HBCDEFGH I JKLMNOPQRS TUVYWXY20123456789 abcdefghijkilmnopgrstuvuxygz _ pH Test Name CODLOQ Press the A or W key to highlight Include as a SmartStart test or Do not include as a SmartStart test and press the enter key Create Test Mame 11 08am 240cti2 Include as a SmartStart test Do not include as a SmartStart test Press f or to select Press ENTER Press ESC to exit Press f or to select character Press i Name to save the test Press the Run Test function key to begin using the test method Refer to the specific reagent chemistry instructions for detailed procedures 36 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 4 Water Analysis Test Menu Water Analysis 11 09am 440cti2 BLANK Test Name CODLOO Saved Measurement Mode Absorbance Wave length ipt Adjust Data File Name More parameters Press f or to select item to change a E a Test Tests Test Running a Test Method Running a Test Method from the USB Stick l eo ee E ii Make sure the test method to be run is in the Thermo folder on the USB stick and the USB stick is connected to the USB port on the front of the AquaMate instrument Press the test key to access the Test Types menu Press the A or Y key to highlight Water Analysis and press the enter key Press the Stored Tests function k
46. specified in these instructions must be used for accurate analysis The majority of AQUAfast reagent methods use a 24mm round vial Cat No AC2V24 or 16mm round vial Cat No AC2V16 Other vial sizes are noted in the individual reagent chemistry instructions 32 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 4 Water Analysis Test Menu Loading and Running a Water Analysis Method on the AquaMate Instrument The USB memory stick included with the AquaMate spectrophotometers contains over 260 preprogrammed methods for use with Orion AQUAfast Merck and CHE Metrics reagent chemistries Methods can be accessed directly from the USB memory stick when connected to the AquaMate spectrophotometer or up to 90 methods can be stored on the instrument for quick and easy access The USB memory stick contains four folders Thermo Orion Merck and CHE Metrics The Thermo folder is empty and all methods to be loaded on the AquaMate spectrophotometer need to be copied to the Thermo folder The Orion Merck and CHEMetrics folders contain preprogrammed methods specific to that manufacturer ne GC D Computer Removable Disk E gt Organize Open Share with Burn New folder Favorites J CHEMetrics J Merck W Desktop Orion Thermo e Information for methods in the Orion folder is available in this user guide and the Thermo Scientific Orion AquaMate Master Methods List document e Information fo
47. stir rod and mix well to dissolve the tablet completely 7 Add one Aluminum No 2 Tablet straight from the foil into the same vial Crush the tablet with a clean stir rod and mix well to dissolve the tablet completely 8 Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial 9 Wait for a reaction period of 5 minutes 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l aluminum Notes Thermo Scientific Before use clean the vials and the measuring beaker with hydrochloric acid approximately 20 Rinse them thoroughly with deionized water To get accurate results the sample temperature must be between 20 C and 25 C A low test result may be given in the presence of fluorides and polyphosphates The effect of this is generally insignificant unless the water has fluoride added artificially Orion AquaMate Spectrophotometer User Manual 51 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P27 Aluminum Powder Pack amp Liquid Test Eriochrome Cyanine R Method 0 01 0 25 mg l Al 1 Load and run the AC4P27 method 2 Use two clean AQUAfast 24mm round vials Cat No AC2V24 and mark one as the blank 3 Pour 20 ml of sample into a 100 ml beaker 4 Add the contents of one Aluminum ECR F20 Powder Pack straight from the
48. straight from the foil into the vial Crush the tablet with a Clean stir rod 8 Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial 9 Wait fora reaction period of 10 minutes 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l ortho phosphate Notes For samples under 5 mg l PO itis recommended to analyze the sample using the AC2095 phosphate ortho low range tablet test method Only ortho phosphate ions react Phosphate in organic and condensed inorganic forms meta pyro and polyphosphates must be converted to ortho phosphate ions before analysis Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed Inorganic forms Organically combined phosphates are converted to ortho phosphate ions by heating with acid and persulfate The amount of organically combined phosphates can be calculated mg l phosphate organic mg l phosphate total mg l phosphate acid hydrolysable The ortho phosphate ions react with the vanadate molybdate reagent under acid conditions to form a yellow colored product Phosphate ortho Phosphorus reactive Orion AquaMate Spectrophotometer User Manual 101 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P95 Phosphate Ortho Powder Pack Te
49. technique menu Orion AquaMate Spectrophotometer User Manual 29 Chapter 3 Orion AquaMate Instrument Setup Locking Unlocking Tests using the Password Use the password 4363797 to lock test parameters so they cannot be overwritten or deleted The password can also be removed to allow editing of the test parameters Note The password cannot be changed Tests stored on a USB memory stick cannot be locked or unlocked Press the utility key to access the Utility menu Press the A or Y key to highlight Stored Tests Directory and press the enter key Press the A or Y key to highlight Load internal test and press the enter key Press the A or Y key to highlight a test and press the Lock Unlock function Key on FPF WD NY e Enter 4363797 as the password using the numeric keypad and press the enter Key Deleting a Test 1 Press the utility key to access the Utility menu 2 Press the A or W key to highlight Stored Tests Directory and press the enter key 3 Press the A or Y key to highlight Load internal test or Load test from USB drive as the test location and press the enter key 4 Press the A or Y key to highlight a test and press the Delete Test function key Using the SmartStart Test Feature The SmartStart feature lets you customize the instrument by placing the most frequently used test methods on the first menu Immediately after the instrument initializes a simple menu containing only the SmartStart tests a
50. that assist with checking the performance of the instrument e Wavelength Accuracy Internal Wavelength Accuracy Standards e Photometric Accuracy e Noise Measurement e Stray Light filters e Stray Light liquids Run the appropriate tests regularly and maintain a log of results to helo document the reliability of the instrument and indicate potential performance Issues Note If a printer is connected enabled and active the instrument automatically prints test results You can also press the print key to print another copy of the results 168 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 9 Performance Verification Accessing the Performance Verification Tests Press the test key to access the Test Types menu Press the A or Y key to highlight Performance Verification and press the enter key Press the A or Y key to highlight the desired test and press the enter key Performance Verification 11 39am 8JuliS avelength Accuracy Internal Wavelength Accuracy Standards Photometric Accuracy Noise Measurement Stray Light filters Stray Light liquids Internal Printer Test Press f or to select test Troubleshooting Checklist Ifa performance verification test fails follow the suggestions below to diagnose common problems If a test continues to fail after you have tried all these recommendations follow the troubleshooting list for the tes
51. the chamber door 13 Press the Measure Sample function key to display the result in mg l ammonia as N Notes e Extremely basic or acidic water samples should be adjusted to pH 7 with a 0 5 mol l 1 N sulfuric acid solution or 1 mol l 1 N sodium hydroxide solution Interference Levels and Treatments Greater than 1000 mg l CaCO3 Interferes at all levels To correct determine the concentration of iron in the sample lron by performing a total iron test Add the same iron concentration to the deionized water step 3 Iron will be blanked out successfully Greater than 6000 mg l CaCO Greater than 100 mg l NO3 N Glycine Less common interferences such as hydrazine and glycine will cause intensified Hydrazine Color colors in the prepared sample Turbidity and color will give erroneous high values Turbidity Samples with severe interferences require distillation 54 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ACR012 Ammonia Low Range Reaction Tube Test Salicylate Method 0 02 2 5 mg I N Ammonia as Nitrogen L 2 J 4 1 8 9 Load and run the ACR012 method Open one 16 mm reaction vial and add 2 ml of deionized water this is the blank vial Open a second 16 mm reaction vial and add 2 ml of sample this is the sample vial Add the contents of one Ammonia Salicylate F5 Powder Pack straight from the foil into each
52. those included on the AquaMate USB memory stick when the test kit manufacturer specifies the wavelength and factor or equation up to third order polynomials required for obtaining the concentration of the test kit When creating a new test method the format for entering the factor or equation Is Result A Bx Cx Dx where x is absorbance at the set wavelength When x Is substituted with Abs the following equation can be used Result A B Abs C Abs2 D Abs where A is a numeric value B is a numeric value multiplied by absorbance C is a numeric value multiplied by absorbance squared D is a numeric value multiplied by absorbance cubed For test kits that specify a simple Pacer Mra lysig 10 17am a multiplicative factor enter the factoras the pesult A BX CX2 DK3 B value A 0 00000000 Bior Factor 12 6800000 For example a colorimetric test kit provides 7 a factor of 12 68 Result Units The equation is Result 12 68 Abs REINE Where Abs is the measured absorbance at the specified wavelength Setthe factor as B 12 68 For test kits that specify an equation decipher the A B C and D factors for the equation For example if the equation given with a Water Analysis 10 29am Z2 Maris test kit is ppm mg L 2301 abs 3 eee 4 ee ee ETT The equation is Result 3 2301 Abs Set the factors as A 3 B 2301 A Etor Factor C D Result Units Hore parameters AO J lI
53. to select in Note The battery backup saves changes automatically even when the instrument power is off Printer Setting 1 Inthe Utility menu press the A or W key to highlight the Printer option and press the enter key to open the printer menu 2 If interfacing the instrument with a computer press the A or W key to highlight the PC option and then press the enter key to set the PC option to On Utility 1 27pm 25Mari5 Software Revision Internal PC External Press f or to select Press ENTER to change Press ESC to exit Stored Tests Directory USB Drive Files Calculator T or to select paranakan 3 If interfacing the instrument with an external printer press the A or Y key to highlight the External option and then press the enter key to set the External option to On 4 Press the esc key to return to the main Utility menu 22 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 3 Orion AquaMate Instrument Setup Paper printouts with text and graphics can be generated using a USB printer attached to the instrument via the USB port To ensure that the instrument can output information correctly to the printer select an appropriate device The instrument is able to print to external printer that Supports HP PCL 5 0 format and later and have printer function only not multi functional printers PCL format does not support HP Windows printers Note The i
54. used with 10mm square vials and the AC203550 method can be used with 50mm rectangular vials All blank and sample volumes must remain the same as those specified in these instructions so Samples may need to be prepared in separate containers and then transferred into the selected vial Vial cleaning As many household cleaners i e dishwasher detergent contain reducing substances the subsequent determination of bromine may show lower results To avoid any measurement errors only use glassware free of chlorine demand Preparation Put all applicable glassware into sodium hypochlorite solution 0 1 g l for one hour and then rinse all glassware thoroughly with deionized water Preparing the sample When preparing the sample the escape of bromine gases i e by pipetting or shaking must be avoided The analysis must take place immediately after taking the sample The DPD color development is carried out at a pH value of 6 2 to 6 5 The reagent tablet therefore contains a buffer for the pH adjustment Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the reagent is added use 0 5 mol l Sulfuric acid or 1 mol l sodium hydroxide Orion AquaMate Spectrophotometer User Manual 57 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate e Exceeding the measuring range Concentrations above 22 mg l bromine can lead to results showing 0 mg l In this event the water sample must be diluted
55. vial Add contents of one Ammonia Cyanurate F5 Powder Pack straight from the foil into each vial Close the vials tightly with the caps and swirl or invert several times to mix the contents Wipe the exteriors of the vials Wait for a reaction period of 20 minutes Place the blank vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank 10 Open the sample chamber door Remove the blank vial from the holder 11 Place the sample vial into the holder in the sample chamber and close the chamber door 12 Press the Measure Sample function key to display the result in mg l ammonia as N Notes Thermo Scientific Strong alkaline or acidic water Samples must be adjusted to approximately pH 7 before analysis use 1 mol l hydrochloric acid or 1 mol l sodium hydroxide If chlorine is known to be present add one drop of 0 1 mol l sodium thiosulfate for each 0 3 mg l Cl in a one liter water sample Iron interferes with the test The interferences can be eliminated as follows Determine the amount of total iron present in the water sample To produce the blank add an tron Standard solution with the same Iron concentration to the vial instead of deionized water Conversion mg l NH mg I N x 1 29 mg l NH3 mog I N x 1 22 Orion AquaMate Spectrophotometer User Manual 55 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ACRO11 Ammon
56. vial 13 Place the vial into the holder in the sample chamber and close the chamber door 14 Press the Measure Sample function key to display the result in mg l total copper Notes Thermo Scientific Alternatively the AC202950 method can be used with 50mm rectangular vials All blank and sample volumes must remain the same as those specified in these instructions so Samples may need to be prepared in separate containers and then transferred into the selected vial Orion AquaMate Spectrophotometer User Manual 75 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P29 Copper Free Powder Pack Test Bicinchoninate Method 0 05 5 mg l Cu 1 Load and run the AC4P 29 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add the contents of one Cu 1 F10 Powder Pack Straight from the foil into the vial 7 Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial 8 Waitfora reaction period of 2 minutes 9 Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample fun
57. 000 Data xls and save the file to the computer desktop 13 Click the Start Overwrite button on the Capture tab of the RealTerm window The program is now ready to receive data from the AquaMate spectrophotometer and export the data to the selected file type name and location Thermo Scientific Thermo Scientific 14 15 16 Chapter 11 Customer Services Fig RealTerm Serial Capture Program 2 0 0 70 D mE Display Port Capture Pins Send EchoPort i2c i2c2 n Clear Freeze Status JE Secs PXD 2 g CAUsers ricki hartwell Desktop AQ8000 Datax v E TD micts s DCD 1 DSR 6 l Ring 9 BREAK Error Press the print key on the AquaMate spectrophotometer to send the data from the instrument to the computer and data collection program Once all data has been sent from the instrument to the computer and data collection program Click the Stop Capture button on the Capture tab of the RealTerm window Open the data file for example AQ8000 Data xls and format the data as needed AquaMate Spectrophotometer Setup for Printer Interfacing The AquaMate printer compatibility requirements are as follows e Printer must be PCL5 or PCL6 e Printer must be a printer only not a multi function printer e Forthis example a PCL5 HP Laser et 1300 printer is used e HP 2055D and Office jet 6000 printers have been used in the past Power on the Orion AquaMate spectrophotometer P
58. 16 Changing the Vial Holder C FOUS Clicsscacworsceisiwessniseariesdnanswescaiaawr du onseisadanseanbabesninadecwmninenins 17 Sample Positioner Setting for the Vial Holder Carousel ccsssessessssssssesssessseeeees 18 O11 Preece rec Eer ote a 20 Orion AquaMate Instrument SetUp ssssssssssssrsnonnnnsnnnnnnnnnnnnnnnnnannnnnnnnnnnnnnnnanannnannnnnnnnana 20 Entering Parameter V GIUCS ssisucsenisdsnsracdaietenishonteiaedehantnecisnddetinadebwciantaduavenienvaransaddedauaitannelantates 20 Setting Utility Menu OPtlons cccccsscssssssscsssecsssessssesessssessseessssessssessseessveesesersaseseeseseseseseten 22 PIRI Ce CUNO arn ae tere asaicr penetra E E E rica beiatntrentias 22 Date Time Setup Setting i giemescntuemnnteisoancins uoetedantes Samiearnubscistneeraiarnuisenaernianosinr unin 24 SNDE o110 0 eee eee ne oer er ee eee 25 Baseline Expralon Seigers eiia ene eee ne 25 BO eFC HIG E E EE ti E E E E E E 26 Seen Conta to CUNO sannindi iine oa O ES 26 Calculator FUNCION snanarkesnarindasantninanietadaninavialnnvariancnanininnuidutinavoaitieetavendennutinnmasts 26 Managing Stored OG 0 rea cn asian crite vatican sbrahcaeataeriatnnninabridincaniornetitee niaies amannuaeeoatins 27 Naming and Saving GT CS oi essasncesscsancntctivateudeuioateciansdeuen etatawiysdmateisasoaretvaensiaae ianiuatins 2 Loading Saved Test FCS wriientsssnnvsinpsicninassatniasneniteianiasidsiievarinsistunb nein ineweiananiinneaiiands 29 Locking Unlocking Tests USING the Password
59. ANK Result Blank 6 Open the sample chamber door and remove the vial from the holder 42 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 4 Water Analysis Test Menu 7 Add the reagent or reagents to the vial Close the vial tightly with the cap and mix the contents Wipe the exterior of the vial 8 Place the vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Rgnt Blank function key to measure the reagent blank Water Analysis 10 10am 4 7MariS Test Mame ACZOO9 BLANK Result ra e Blank tgant Blank 10 Open the sample chamber door and remove the vial from the holder 11 Empty and rinse the vial and then fill the vial with sample Add the reagent or reagents to the vial Close the vial tightly with the cap and mix the contents Wipe the exterior of the vial 12 Place the vial into the holder in the sample chamber and close the chamber door 13 Press the Measure Sample function key to display the results Water Analysis Test Name ACZOOD Result Measure as Measure Measure Blank Rant Blank Sample Water Analysis 10 12am 4 7MariS Test Mame ACZOO9 BLANK Result Thermo Scientific Orion AquaMate Spectrophotometer User Manual 43 Chapter 4 Water Analysis Test Menu Creating or Modifying a Test Method How to Define the Test Method Parameters The Water Analysis test technique can be used with colorimetric reagents in addition to
60. Absorbance Transmittance and Concentration Measurements Using the Conc Factor Mode to Measure Concentration In this mode a concentration factor is used to determine the concentration of samples When the factor is entered the Abs T of the sample is measured and the sample concentration is calculated from the entered factor The measurement can be expressed mathematically as Abs T of Sample Factor Concentration of Sample 1 Ifnecessary press the Change Mode function key until Conc Factor is set as the mode 2 Ifnecessary press the Set nm function key to set the wavelength value Use the numeric keypad to enter the wavelength value for taking measurements Press the Set nm function key again to save the entered wavelength value 3 Press the Units Factor function key to set the units and enter the factor a Press the Enter Factor function key use the numeric keypad to enter the value of the factor and press the enter Key b Press the Select Units function key press the A or W key to highlight the desired units and press the enter key Conc with Factor 3 20pm a Factor 1 850 Units ppr Press f or to select item to change ee et Factor Units 4 Press the esc key to return to the main measurement mode 5 Prepare the blank place it in the B position and press the Measure Blank function key Once the blank has been measured remove it from the carousel 6 Place the sample in the appropriate position o
61. AquaMate 7000 replacement Tungsten lamp in pre aligned fixture Bromine DPD method tablet reagent 100 tests 16 mm round vials 10 pack Alkalinity P acid indicator method tablet reagent 100 tests Aluminum eriochrome cyanine R method tablet reagent 50 tests Chlorine total high range KI acid method tablet reagent 100 tests en a a a gt gt gt en ee ee a a ee gt D gt gt D gt gt MI MIE MIE MIE DMDIDIDIP NDI DNInInImnanitIini t m MI AI OLlLOlLo o oslyolro o O OI O wU INI N I NI N N wi D A N Bil nN wl rm NINJ rR nI NI Oloolu lelol l ol l 4 CO oOololololololo lo alvlolvlol ol o lt lt lelInlAelz IlIZlciojlo o ol l 2 D SP at re w ES SB retlrmol njolo meilrn ao aolo mnl zazlcolrYl o D Thermo Scientific Orion AquaMate Spectrophotometer User Manual 199 Chapter 11 Customer Services Cat No Description Hydrazine 4 dimethyl amino benzaldehyde method powder reagent 30 tests AC2046 Nitrite as nitrogen n 1 naphthyl ethylenediamine method tablet reagent 100 tests AC4P 46 Nitrite as nitrogen low range diazotization azo method powder reagent 100 tests 200 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Chapter 11 Customer Services Cat No Description AC4P 60 Silica high range silicomolybdate method powder reagent 100 tests AC4P 82 Sulfate barium sulfate turbidity method powder reagent
62. AquaMate Spectrophotometer User Manual 159 Chapter 8 Absorbance Transmittance and Concentration Measurements Taking Measurements with Absorbance Difference Method Once the parameters have been set for the absorbance difference test 1 Press the Run Test function key 2 Prepare the blank and samples that will be measured 3 If Auto 6 or Auto 3 is selected for the Sample Positioner setting a Place the blank and samples in the appropriate positions on the carousel b Confirm that the blank and samples are in the appropriate positions on the carousel and press the enter key c Once the blank and samples have been measured remove them from the carousel d To measure additional samples press the Measure Sample function key and repeat Steps 3a through 3c 4 If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting a Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank has been measured remove it from the carousel b Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Sample function key Repeat for each sample 5 When all of the samples have been measured press the Save Data function key and follow the onscreen prompts to save the sample measurements to the USB memory Stick Recalling an Existing Absorbance Difference Meth
63. C2065 Zinc Tablet Test Zincon Method 0 02 1 mg l Zn l Load and run the AC2065 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of deionized water Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Empty and dry the vial and then fill the vial with 10 ml of sample 6 Add one Copper Zinc LR Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 7 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 8 Wait for a reaction period of 5 minutes 9 Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Rgnt Blank function key to measure the reagent blank 11 Open the sample chamber door and remove the vial trom the holder 12 Add one EDTA Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 13 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 14 Place the vial into the holder in the sample chamber and close the chamber door 15 Press the Measure Sample function key to display the result in mg l zinc Notes Reverse color methods use a reagent that when prepared w
64. Clear Freeze Display As Ta Half Duplex Status a baal newLine mode Disconnect C Hex space Invert f 7Bits PXD 2 fa Ascii v TXD 3 uint C is Data Frames CTS 8 C Hex aT C inti Bytes zi DCD 1 m re Single Gulp DSR 6 C Binary Ring 9 C Nibble Rows BREAK C Float i 14 O Hex CSV Terminal Font gt Scrollback Error You can use ActiveX automation to control me Char Count 1916 CPS 0 Port 9 19200 8N1 None 10 Once the connection has been validated select the type name and location of the file the data will be saved as 11 Click the Capture tab on the RealTerm window RealTerm Serial Capture Program 2 0 0 70 Display Port Capture Pins Send Echo Port 12C 120 2 l2CMisc Misc n Clear Freeze Capture Eme Diagnostic Files status Start Append Bytes o000000 Log hex Disconnect File c temp capture tt M t Secs Trace hex teas a EEE Direct Capture V TXD 3 Clear Dump CTS 8 Capture as Hex TimeStamp Delimiter DCD None C Matlab comma File o 1 C Unix YMDHS _ DSR 6 C UnixHex t space realtermlog v Ring 3 BREAK Error You have to click in terminal window before you can Char Count 1916 CPS 0 Port 9 19200 8N1 None 12 Click the button next to the File field and set the file type name and location For example use an Excel xls file type name the file AQ8
65. Confirm that the RealTerm communication settings match the appropriate AquaMate communcation settings Thermo Scientific Orion AquaMate Spectrophotometer User Manual 191 Chapter 11 Customer Services 5 r 4 RealTerm Serial Capture Program 2 0 0 70 ial Px Display Port Capture Pins Send EchoPor i2c 12c 2 l2cMisc n Clear Freeze Status Baud 57600 Port 3 v Open Spy S Change Connected PXD 2 F Software Flow Control Parity Data Bits Stop Ae moe Receive Xon Char 1 TXD 3 te None Shits 1 bit C 2bits E cTs 8 m F C 7bits Hardware Flow Control Transmit Xoff Char 19 DCD 1 eo s 6 bits None C RTS CTS TE DSR 6 C Space 5 bits B DTR DSR C R5485 rts C Raw Ring 9 fe Telnet BREAK Error You have to click in terminal window before you can Char Count 0 CPS 0 Port 3 57600 8N1 None Click the Port dropdown menu and select the COM port number of the Spectrophotometer USB connection 4 RealTerm Serial Capture Program 2 0 0 70 e PX Display Port Capture Pins Send EchoPor i2c 12c2 12CMisc Misc n Clear Freeze Status Baud 57600 _y Port Em v Open Spy Jf Change Iv Connected _ 3 f0 Software Flow Control Tao Parity Data Bits 9 Serial2 Receive Xon Char 17 TAD 3 None 8bits 0 E crs 6 C Odd C 7bits WCPO Transmit Xoff Char 19 i DCD 1 C Even
66. Curve Fit Number of Standards Units q sample Positioner Manual ore parameters Press f or to select item to change satin ot ee Standards Test Tests Orion AquaMate Spectrophotometer User Manual 123 Chapter 6 Standard Curve Test Menu Standard Curve 10 19am eo IDH O OFF Auto Save Data More parameters Press f or to select item to change totes Got ee Standards Test Tests 4 Press the Save Test function key and follow the onscreen prompts to save the test Measuring Standards for a Standard Curve Once the parameters have been set for the standard curve 1 Press the Run Standards function key 2 Prepare the blank and standards that will be used to create the standard curve 3 If Auto 6 or Auto 3 Is selected for the Sample Positioner setting a Place the blank and standards in the appropriate positions on the carousel b Foreach standard use the numeric keypad to enter the concentration of the standard and press the enter key to save the value c Press the Measure Standard function key Confirm that the blank and standards are in the appropriate positions on the carousel and press the enter key Standard Curve 10 52am 15ApriS Test Name BLANK Conc Insert blank into position E Insert standards 1 into positions 2 amp 4 Press ENTER to measure 4 If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting a Foreach standard use the numeric
67. D Method 0 02 2 mg l 0 Ozone Measurement in Absence of Chlorine l 2 ae ee N Load and run the AC3048 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber Close the sample chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Empty the vial leaving a few drops of sample remaining in the vial Add one DPD No 1 Tablet and one DPD No 3 Tablet straight from the foil to the vial Crush the tablets with a clean stir rod Add sample to the 10 ml mark on the vial Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial 10 Place the vial into the holder in the sample chamber Close the sample chamber door 11 Wait for a reaction period of 2 minutes 12 Press the Measure Sample function key to display the result in mg L ozone Ozone Measurement in Presence of Chlorine 2 Aa pe et se w Load and run the AC 3048 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber Close the sample chamber door Press the Measure Blank function key to measure the
68. DNS reagent solution contains arsenite Chlorine concentrations up to 5 mg l do not interfere Seawater and wastewater samples must be distilled Orion AquaMate Spectrophotometer User Manual 79 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC3032T Hardness Total Tablet Test Metallphthalein Method 2 50 mg l CaCO3 Low Range or 20 500 mg l CaCO High Range For Low Range Total Hardness Measurements L 2 ot a W 8 9 Load and run the AC3032TL method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one Hardcheck P Tablet straight from the foil into the vial Crush the tablet with a clean stir rod Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial Wait for a reaction period of 5 minutes Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l total hardness For High Range Total Hardness Measurements l 2 Sa a a 8 9 Load and run the AC3032TH method Filla clean AQUAfast 24mm rou
69. Get See Standard Test Tests 5 Press the Save Test function key and follow the onscreen prompts to save the test Measuring a Standard with Advanced A T C Method Once the parameters have been set for the concentration test and the measurement mode Is set to Conc Std 1 Press the Run Standard function key 2 Prepare the blank and standard that will be measured 3 Use the numeric keypad to enter the concentration of the standard and press the enter key to save the value 4 f Auto 6 or Auto 3 is selected for the Sample Positioner setting a Place the blank and standard in the appropriate positions on the carousel b Press the Measure Standard function key Confirm that the blank and standard are in the appropriate positions on the carousel and press the enter key Thermo Scientific Orion AquaMate Spectrophotometer User Manual 151 Chapter 8 Absorbance Transmittance and Concentration Measurements 5 IfManual 6 or Single Cell Holder is selected for the Sample Positioner setting a Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank has been measured remove it from the carousel b Place the standard in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Standard function key and press the enter key to measure the standard 6 When the standard has been measured
70. Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Instrument Setup Turn on the spectrophotometer Choose a Suitable cell size and method for the organic carbon content expected in your samples Refer to the following chart Select the desired cell holder position 1 or 5 cm by pressing the cell position key For 10 cm install the carousel with 10 cm holder Access the USB memory stick using a computer Copy the desired preprogrammed method from the Orion folder to the Thermo folder See Note 1 Remove the USB memory stick from the computer and insert it into the USB port on the front of the AquaMate Select the method and load it Press the Run Test function to start the analysis Refer to the following chart for choosing the method Sample Organic Carbon Organic Carbon Organic Carbon Concentration gt 0 5 mg L gt 0 1 mg L gt 0 05 mg L et Cell Size See Note 2 1 cm 10 mm 5 cm 50 mm 10 cm 100 mm Method Name UV254 1 UV254 5 UV254 10 Zero the Instrument Spectrophotometer Check 1 Touching only the frosted sides of the cell rinse a clean cell three times with DI water Then fill with DI water Use a lint free wiper to remove water on the outside 2 Open the sample compartment and insert the sample cell containing DI water the blank into the sample holder with the clear sides facing front and back If sample cell is not in the light path press the correct sample pos
71. No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Sulfide No 1 Tablet straight from the foil into the vial Crush the tablet with a clean Stir rod 7 Add one Sulfide No 2 Tablet straight from the foil into the same vial Crush the tablet with a Clean stir rod 8 Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial 9 Wait fora reaction period of 10 minutes 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l sulfide Notes The tablets must be added in the correct sequence Chlorine and other oxidizing agents that react with DPD do not interfere with the test To avoid loss of sulfide collect the sample carefully with a minimum of aeration It is essential to test the sample immediately after collection The sample temperature should be 20 C A different temperature can lead to higher or lower results 112 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate A
72. P Tablet straight from the foil into the vial Crush the tablet with a clean stir rod Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Sample function key to display the result in mg l total alkalinity Notes The terms alkalinity p p value and alkalinity to pH 8 2 are identical For accurate test results exactly 10 ml of water sample must be taken for the test This method was developed from a volumetric procedure for the determination of alkalinity p Due to undefined conditions the deviations from the standardized method may be greater 50 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2027 Aluminum Tablet Test Eriochrome Cyanine R Method 0 01 0 3 mg l Al 1 Load and run the AC2027 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Aluminum No 1 Tablet straight from the foil into the vial Crush the tablet with a clean
73. St ssssssssrsrsrsrsrnrnrrsrsrsrnrnrnrrsrsrsrrnrnrerrererererrerns 66 ACAP 72 Chlorine Total Powder Pack iOS Cae cec ists doa cndcnsactsatavectnantinadaitctianacenadaawnatiunt 67 AC3072 Chlorine Total High Range Tablet TeSt cccscsssssssssssssssessssessseersseeess 68 AC2099 Chlorine Dioxide Tablet POS tsetse scuswsetoacandtauat eal seatsaideandunesountaniiielimneadectiuls 69 CODLOO COD Low Range Digestion Tube TeSt ceecsssessssserssssersssrerssseerssssereess 11 CODHO00 COD Mid Range Digestion Tube TeSt s ssssssssssrssrsrsrnsrsrnrnrrnrnrnsrnrnrnsrererrnreres 13 CODHPO COD High Range Digestion Tube TesSt ssssisrsrrrrsrrrrrrrsrnrrrrrrrrrrerererrrnnnns 14 AC2029 Copper Free amp Total Tablet Test ccssecssscssssssrssrsrssressserssseeserseseen 15 AC4P 29 Copper Free Powder Pack TeSt s ssssssssssrerssrsrsrnrrnnsrnrnnrnrnrnnrrnresrerenrnrererens 76 AC2098 C yanune Acia Tablet TES secaviesssessnssssnnaraarvaneanmwnsatoan paN 11 AC 2009 Fluoride SPADNS Liquid TeSt s ssssssssssrsrnsrsrnsrsrnrnunnnsnnrnrnrnnnnrnnnrnrnsrsrnrsrnrerenrsrns 78 AC3032T Hardness Total Tablet Test tisicscasidustavewcanicuinee adenanesmuanlennvindeanseneni ade 80 AC2030 Hydrazine Powder ToS tincnnonnnroanannanaa 81 AC 207 BOM Gel Tablet OG Gs caccnscthinsatscarastteetaiasug eis esiaak gncaeaie teatmanieaaatameateieate 82 AC4P 78 Iron Ferro Powder Pack T St scsssssssssssrssssssrsssssssssesssessstrserserseseess 83 AC4P 79 Iron Total Powder Pac
74. TS bran cic ie E Space 5 bits C DTR DSR C RS485 rts C Raw Telnet Brim n Clear Freeze Status Disconnect PXD 2 CPS 0 Char Count 0 7 Port 9 19200 8N1 None Click the Display tab on the RealTerm window Select the Ascii option in Display As field m RealTerm Serial Capture Program 2 0 0 70 Display Port Capture Pins Send EchoPort l2c l2c 2 I2CMisc Mise Display As 7 Half Duol apron ag Half Duplex s aSo newLine mode C Hexfspace Invert 7Bits Hex Ascii 7 uint pi Data Frames ex a intB Bytes IE l Asci Single _Gulp m Pinan Rows Cols C Float4 i 16 Seo S Hex CSV Terminal Font Scrollback Brc n Clear Freeze Status Disconnect You have to click in terminal window before you can Char Count 0 CPS 0 Port 9 19200 8N1 None When data from the AquaMate spectrophotometer is ready to be sent to the computer first test the connection between the spectrophotometer and data collection program Spectrophotometer should appear on the program display Press the print key on the AquaMate spectrophotometer The data from the AquaMate Orion AquaMate Spectrophotometer User Manual 193 Chapter 11 Customer Services 194 Orion AquaMate Spectrophotometer User Manual aa RealTerm Serial Capture Program 2 0 0 70 Display Port Capture Pins Send EchoPort 2c 126 2 I2CMisc Misc n
75. Total Digestion Tube Te St csssessssessssecrsssterssserersssrees 104 ACDO95AH Phosphate Acid Hydrolysable Digestion Tube Test essnee 106 AC 2060 5 ica Taplet TeS Eis meinn eect Gut oe ese tahoe ati daa alaslealaheiieva weal 108 AC2061 Silica with Phosphate Removal Tablet TeSt 109 ACEP OO Silica Powder Pack TSU vicctiuteiecte tities elwpechubbertatuatdbeahiitianitdeldaubeantieaudbandiiadatites 110 AC4P 82 Sulfate Powder Pack CSU aricacsustese vbcacnoiaune nin snataniatisstienanodaianideaann tantuab tania 111 AC2016 SUMGS TaDlCULCSE sicanttiecrscetcat wale sraaars tal auuacect ahaa ear gia mmraceiess cel ahaes 112 AC 2065 ZING TANEC CSU rana a 113 Color Measurement from Application LOG 131 sssssssrsrsrsrsrnrrnrsrsrnrnrnrnrrsrererernrrrrnrererenns 114 UVA and UV254 Measurements from Application Log 137 cccsssesesesssseieen 118 Cia Dlel O iriran unineme Uieai 122 Standard Curve Test Menu siiisiasiatsinccsicauscaatesnsrtdtsnisrasicrsnvassinansinsssunsaticsumaaiennruananin 122 Concentration Measurements using the Standard Curve Application Custom Method 122 Setting the Parameters for a Standard CUVE c ccseccsssserssssserssssersssssereessserersssererseses 123 Measuring Standards for a Standard CU taisatiitrristiaariniapiaratiutanalida dimmcastie anaes 124 Measuring Samples using a Standard Curve cccsssecsssssssssesssssserssssserssssesersssererseses 126 Eding a standard CUNE oaair ecieaiaanto rains oatatchan tase heaeensanera
76. ak in the graph Scanning Calculator Enables the calculator mode Utility Cell Position Displays the vial or cuvette position placed in the light path only with Auto 6 or Auto 3 sample positioner settings 202 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Appendix A General Instrument Information Change Mode Change to Abs Change to T Switches measurement modes Basic A T C and some Performance Verification tests Collect Baseline Starts the collection of the baseline Scanning Concentration Sets the concentration value Conc of Standard Displays the entered concentration value Adv A T C Cursor Goes to cursor tracking mode to view data points in graph Kinetics Scanning lt Cursor Moves the cursor right or left on the graph and displays the data of each point Cursor Kinetics Scanning Curve Fit Selects the type of line fit calculation Standard Curve Data File Name Allows entry of a name for the data file when AutoSave is on Date Standards Displays the date when standards were last measured Standard Curve Measured Date Time Setup Enters the current date and time settings for the instrument Utility Delay Time Enters the time from test initiation to first measurement allows for sample equilibration Adv A T C and Kinetics Delete Character Deletes the last character of name entry Test Name and E
77. al 17 Chapter 2 Spectrophotometer Basics Sample Positioner Setting for the Vial Holder Carousel The instrument allows the use of various vial holder carousels to take measurements When selecting the test parameters the Sample Positioner setting determines how the instrument will work with the installed vial holder carousel to obtain readings in the test measurement screen The Sample Changer keys on the instrument keypad are also be used for certain settings Sample Positioner Settings Water Analysis 4 20pm 25Mar15 Advanced A xT C 4 17pm 25Mari15 BLANK BLANK Test Name Test Name 1 Cell Platform Measurement Mode Measurement Mo Manual Wave length Auto 3 Press T or to select IDH CO OFF 1 Number of Samp Press ENTER Statistics Auto Print More parameters Auto Save Data More parameters Press t or to select item to change Press t or to select item to change Sa a Test Tests Test This setting is only used in the Water Analysis test type Manual 3 Use the Sample Changer keys to position the carousel with the desired vial holder in the beam pathway for measurement Positions B 2 and 4 are selectable Measure one blank and five samples without changing vials on the carousel The instrument automatically measures the blank and advances the carousel to measure the remaining samples in positions 1 through 5 Measure one blank and two samples without changing vials on the carousel The instrument automatically me
78. al tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add six drops of Phenol Red Solution to the vial Add drops of the same size by holding the bottle vertically and squeezing slowly 7 Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial 8 Place the vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Sample function key to display the result in pH units Notes Thermo Scientific When testing chlorinated water the residual chlorine contents can influence the color reaction of the liquid reagent This can be avoided without interfering with the pH measurement by adding a small crystal of sodium thiosulfate Na2S203 5 H20 to the Sample before adding the phenol red solution Phenol red tablets already contain thiosulfate Due to differing drop sizes results can show a discrepancy in accuracy by comparison with tablets This can be minimized by using a pipette 0 18 ml phenol red solution is equivalent to 6 drops After use replace the bottle cap securely Store the phenol red solution in a cool dry place ideally at between 6 C and 10 C Orion AquaMate Spectrophotometer User Manual 99 Chapter 5
79. alculated mg l phosphate organic mg l phosphate total mg l phosphate acid hydrolysable Application for water wastewater and seawater Highly buffered samples or samples with extreme pH values should be adjusted between pH 2 and pH 10 before analysis with 1 mol l hydrochloric acid or 1 mol l sodium hydroxide Phosphate ortho Phosphorus reactive Interferences Large amounts of turbidity may cause inconsistent results Interference Level Interference Level greater than 200 mg l greater than 300 mg l at any level Silica Silicium dioxide greater than 50 mg l greater than 100 mg l greater than 10 mg l greater than 10 mg l Sulfide greater than 100 mg l greater than 80 mg l 102 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ACR095 Phosphate Ortho Reaction Tube Test Phosphomolybdenum Ascorbic Acid Method 0 06 5 mg l PO L 2 oro pe a 8 9 Load and run the ACR095 method Open one 16 mm P04 P Dilution Tube and add 5 ml of sample Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door Remove the vial from the holder Add the contents of one Phosphate Rgt F10 Power Pack straight from the foil into the vial Use a funnel to add the reagent
80. ame SCAN_O1 BLANK Select Data Save Data Press f or to select Press ENTER me Eae Ee Ere Ere Eee Ere Ere Ere Eee e e 0 0 0 0 0 IDH 1 paseme collected T 3 Select the scan data a To use all the scan data press the Use all data function key b To select specific start and end wavelengths press the A or Y key to highlight the appropriate data point in the table for the starting wavelength press the Start nm function key press the A or W key to highlight the appropriate data point in the table for the ending wavelength and press the End nm function key Scanning 3 08pm 16Apris Test Name SCAN_O1 BLANK Wave length T D O o E a o a o IDH Baseline collected 16Apri5S 4 The instrument will highlight the selected data points To display the graph using the highlighted data points press the Graph function key 140 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 7 Wavelength Scanning Test Menu Recalling an Existing Scanning Method 1 Press the test key to access the Test Types menu 2 Press the A or Y key to highlight Scanning and press the enter key Test Types 3 53pm 15Apris Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics a 3 Point et Multiwavelength Performance Verification Press f or to select ee De a Start Tests ATC 3 To recall an exi
81. ample have been measured remove them from the carousel To measure additional samples press the Measure Sample function key and repeat steps 3a through 3c If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting qd Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank has been measured remove it from the carousel Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Sample function key After the measurement is complete the kinetics data and rate will be shown Press the Tabular or Graph function key to switch between tabular and graphical displays d On the graphical display press the Edit Graph function key and then press the Cursor function key to move the cursor line from one position to another on the plot As the cursor moves the rate and result values indicate the values for the point where the cursor is located Recalling an Existing Kinetics Method Press the test key to access the Test Types menu l 2 3 4 Thermo Scientific Press the A or Y key to highlight Kinetics and press the enter key To recall an existing Kinetics method press the Stored Tests function key Press the A or Y key to highlight Load internal test or Load test from USB drive and press the enter key Press the A or Y key to highligh
82. and Y axes so all the data appears on the plot The instrument also recalculates the results using all the data and displays them To use Manual Scale 1 With kinetics data displayed on the Edit Graph screen press the Manual Scale function key to display the manual scale options 2 Enter the appropriate minimum or maximum value for the X or Y axis and press Min Y Max Y Min X or Max X to accept it The instrument redraws the plot using the entered minimum and maximum values and displays the recalculated rate and result 3 Continue until you have entered all the values that you want to change To use the Cursor function 1 With kinetics data displayed on the Edit Graph screen press the Cursor function key to display the cursor options 2 Press the Cursor or Cursor function key to position the cursor line on the appropriate point on the graph The data for the selected point will be displayed 3 When the cursor line is in the correct position press the Set Min X or Set Max X function key to accept the selected point 4 The instrument will redraw the plot using the selected minimum and maximum values and display the recalculated rate and result 166 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements Rescaling and Recalculating Tabular Kinetics Results The Kinetics application allows the viewing and manipulatio
83. ank is a mixture of the reagent and deionized water and provides a zero concentration point with the darkest color highest absorbance The color of samples prepared with the reagent will decrease as the concentration increases for this method The same batch of SPADNS reagent solution must be used for testing reagent blank and Sample measurement and one point calibration procedures The one point calibration process needs to be performed for each new batch of SPADNS reagent solution see Standard Methods 20 ed 1998 APHA AWWA WEF 4500 F D 4 a 78 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate During testing blank reagent blank and sample measurement and one point calibration procedures the same vial should be used as different vials may exhibit minor tolerances The calibration solution and water samples should have the same temperature 1 C As the test result is highly dependent on exact sample and reagent volumes the sample and reagent volumes should always be measured using a 10 ml or 2 ml volumetric pipette class A The accuracy of the test methods decreases above a level of 1 2 mg l fluoride Although the results are sufficiently accurate for most applications more exact results can be achieved using a 1 1 dilution of the sample prior to use and subsequent multiplication of the result by 2 SPA
84. aracter press the Add Character function key to select the highlighted character and repeat until the desired name has been entered shown c To save the entered test name press the Accept Name function key 3 The instrument will return to the test parameters menu Water Analysis 11 22am 46Mari5 BLANK Test Name easurement Mode Wave length ipt Adjust Reverse Color sample Positioner IDH O OFF statistics More parameters Press f or to select item to change C T Test Tests Test 4 Press the Save Test function key to save the test name and test parameters a Press the A or W key to highlight Save as an internal test Save test to the USB drive or Save test to internal and USB and press the enter key b Press the Accept Name function key Ifthe test name already exists press the enter key to overwrite the existing test or press the esc key to cancel and rename the test with a unique name c Press the A or W key to highlight Include as a SmartStart test or Do not include as a SmartStart test and press the enter key 5 The testname will be shown with Saved after the name 28 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 3 Orion AquaMate Instrument Setup Water Analysis 11 25am 46Mari5 BLANK Test Name TEST_12345 Saved easurement Mode Wave length Auto Save Data More parameters Press f or to select item to change a Test Tests
85. arsussiey tapsivannisanenitaeiieaal 46 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate sss 46 Orion AQUAfast Colorimetric Reagents Compatible with Orion AquaMate Instruments 46 Orion AQUAfast Reagent IMS UUCTIONS sisccccvosasciianitaceiaidiar iwi tartinnargebiancannaveteainncedaaanennan 48 Recommendations for Avoiding Measurement ErrOls ccscsssesscsseessssessseeessereesssseasen 48 AC2002 Alkalinity M Alkalinity to pH 4 3 Tablet TeSt 49 AC3002P Alkalinity P Alkalinity to pH 8 2 Tablet TeSt 50 AC 2027 Aluminum Tablet TeSt nsuipininarnnnaa ana 51 AC4P 27 Aluminum Powder Pack amp Liquid TeSt sssssssisrsrerrsrsrrsrnrnrrrrsrrrernrrrrrrererererrern 52 AC 2012 AMMONT Tablet TES Uaiassss shots cca a ubmdues sabre nagaetauncs 53 ACEP 12 Ammonia Powder Pack TCS Ui clini icateothavis carseat cnn seeaintcatt ianstehaataiciatuanmenaianeane 54 ACRO12 Ammonia Low Range Reaction Tube Te St ccccssssessssesesssssssrseerseees 55 ACR011 Ammonia High Range Reaction Tube TeSt s ssssssrsrrrsrrrrrrrrrrrrrrerrrnrnn 56 AC 2035 Bromine Tablet TeS tniasdpudupssi oaea 57 AC201I7 Chonde Tablet TES tsnena a 59 AC2070 Chlorine Free amp Total Tablet TeSt s s sssssssssrsrssrsresrnrnsrnrrsrnsnnrnsinsrrnsreresrnrenns 60 AC2071L Ghlorine Free Tablet T OS Uiccci seis Sen Shas soucicia anc adetaasineteas each aadaahiv wienatsalanantan asl anaunt 62 AC2072 Chlorine Total Tablet Tes iniinom 64 AC4P 71 Chlorine Free Powder Pack Te
86. asures the blank and advances the carousel to measure the samples in positions 2 and 4 Measure the blank and up to five samples on the carousel Use the Sample Changer Keys to position the carousel to the appropriate position for Sample measurement Place the blank vial in the B position and samples in position 1 5 Regardless of the carousel position the carousel automatically goes to the B position and measures the blank when the Measure Blank function key is pressed Use the Sample Changer keys to select different positions for samples Measure the blank and all samples using one position on the carousel 1 Cell Place the blank in the holder measure the blank remove the blank place a sample in Platform the holder measure the sample remove the sample and repeat for all samples The Sample Changer keys do not function is this setting Note When the carousel is installed and Manual 6 Is selected the instrument always considers the vial in the B position as a blank This means that even after measuring the blank the first time Samples can only be placed in positions 1 through 5 18 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 2 Spectrophotometer Basics Three Position Vial Holder Carousel Corresponding Sample Changer Keys SN Wee i f ii TR T Ch Ss a K gt t s I L x N JS aff o ay 10 io si im I ij w any i 3 v
87. blank 5 Open the sample chamber door and remove the vial from the holder 6 Add the contents of one Manganese Citrate Buffer Powder Pack straight from the foil into the vial 7 Close the vial tightly with the cap and swirl or invert several times to mix the contents 8 Add the contents of one Sodium Periodate Powder Pack straight from the foil into the vial 9 Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial 10 Wait for a reaction period of 2 minutes 11 Place the vial into the holder in the sample chamber and close the chamber door 12 Press the Measure Sample function key to display the result in mg l manganese Notes This test is applicable for the determination of soluble manganese in water and wastewater Highly buffered water samples or extreme pH values may exceed the buffering capacity of the reagents and requires sample pre treatment If samples were acidified for storing adjust the pH between 4 and 5 with 5 mol l 5 N sodium hydroxide before test Do not exceed pH 5 as manganese may precipitate Interferences Interfering Substance Interference Level Greater than 700 mal Chloride Greater than 70 000 mg l Greater than 5 mal Greater than 100 000 mg Orion AquaMate Spectrophotometer User Manual 87 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P42 Molybdate Powder Pack Test Mercaptoacetic Acid Me
88. blank Open the sample chamber door and remove the vial from the holder Empty the vial leaving a few drops of sample remaining in the vial Add one DPD No 1 Tablet and one DPD No 3 Tablet straight from the foil to the vial Crush the tablets with a clean stir rod Filla second clean AQUAfast 24mm round vial with 10 ml of sample Add one Glycine Tablet straight from the foil to the vial Crush the tablet with a clean stir rod Close the vial tightly with the cap and swirl several times until the tablet is dissolved 96 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate 9 Transfer the contents of the vial with glycine solution into the vial containing the DPD No 1 and DPD No 3 tablets 10 Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial 11 Place the vial into the holder in the sample chamber Close the sample chamber door 12 Wait for a reaction period of 2 minutes 13 Press the Measure Sample function key to display the result in mg L ozone Notes Vial cleaning As many household cleaners i e dishwasher detergent contain reducing Substances the subsequent determination of ozone may show lower results To avoid any measurement errors only use glassware free of chlorine demand Preparation Put all applicable glassware into a
89. c acid in the tablets Ortho phosphate ions react with the reagent to form an intense blue color Phosphate in organic and condensed inorganic forms meta pyro and polyphosphates must be converted to ortho phosphate ions before analysis Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed Inorganic forms Organically combined phosphates are converted to ortho phosphate ions by heating with acid and persulfate The amount of organically combined phosphates can be calculated mg l phosphate organic mg l phosphate total mg l phosphate acid hydrolysable Phosphate ortho Phosphorus reactive 100 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2096 Phosphate Ortho High Range Tablet Test Vanado Molybdate Method 1 80 mg l PO 1 Load and run the AC2096 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Phosphate HR P1 Tablet straight from the foil into the vial Crush the tablet with a Clean stir rod 7 Add one Phosphate HR P2 Tablet
90. c com waterlibrary or call Technical Support 2 Alternately use disposable cuvettes formulated for UV measurements available in the 1 cm 10 mm cell path Thermo Scientific Orion AquaMate Spectrophotometer User Manual 121 Standard Curve Test Menu Concentration Measurements using the standard Curve Application Custom Method The Standard Curve test technique is used to perform a quantitative analysis experiment using a multipoint calibration curve A calibration curve is composed of standards of well known concentration A fit of this standard curve Is used to measure the concentration of samples This test technique is ideal when using colorimetric reagents for which the test kit manufacturer does not specify a factor or equation for obtaining the concentration of the test kit Always follow the reagent instructions provided by the test kit manufacturer when creating a custom method Note If the manufacturer does not specify the wavelength for the test kit the Scanning test technique should be used with a prepared standard to determine the wavelength parameter for the reagent before creating a standard curve Use Standard Curve Test Technique e To create a standard curve by setting parameters and measuring standards for the curve e To view calibration curve data select between graphical and tabular displays e To edita standard curve including changing the number of standards selecting a different curve fit or deleting point
91. ce our website and other Thermo Scientific web locations These notes give detailed information on how to test for color on our Thermo Scientific Orion AquaMate spectrophotometers AQ 7000 visible range and AQ8000 UV Vis range Application Notes available at this time include e Log 133 Color of water and wastewater by Pt Co method at 455 nm e Log 134 Color of pulp mill wastewater by Pt Co method at 465 nm NCASI method References 1 Standard Methods for the Examination of Water and Wastewater Method 2120B www standardmethods org 2 Standard Methods for the Examination of Water and Wastewater Method 2120 www standardmethods org 3 Available through Fisher Scientific at www fishersci com 4 NCASI Technical Bulletin No 253 Dec 1971 See 40CFR Part 136 Table IB footnote 18 WWW eCcfr gov 5 EPA Color Method 110 1 htto www umass edu tel mwwp acrobat epal10 1colorspec pdf Thermo Scientific Orion AquaMate Spectrophotometer User Manual 117 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate UVA and UV254 Measurements from Application Log 137 UVA and UV254 Measurement of Water This method utilizes a spectrophotometer to measure the absorbance of waters such as drinking water and source water at 254nm wavelength These results may be correlated to Organic carbon color and or disinfection byproduct precursors Results can also indicate the efficacy of treatment processes that remove organic carbo
92. ces gj IDE ATA ATAPI controllers g IEEE 1394 Bus host controllers Keyboards P Mice and other pointing devices lt 4 Modems KX Monitors E Network adapters i Other devices Spectrophotometer I Portable Devices Ports COM amp LPT o Processors gt Smart card readers Sound video and game controllers lt gt Storage controllers iM System devices Universal Serial Bus controllers gy WI 6 Click the Update Driver button on the Spectrophotometer Properties window 188 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 11 Customer Services lia Spectrophotometer Device type Other devices Manufacturer Unknown Location Port_ 0003 Hub_ 0004 Device status The drivers for this device are not installed Code 28 There is no driver selected for the device information set or element To find a driver for this device click Update Driver vY 7 Click the Browse my computer for driver software button How do you want to search for driver software gt Search automatically for updated driver software Windows will search your computer and the Internet for the latest driver software for your device unless you ve disabled this feature in your device installation settings gt Browse my computer for driver software Locate and install driver software manually 8 Click the Browse button Browse for driver software on your compu
93. cientific Chapter 11 Customer Services Instrument Specifications Orion AquaMate 8000 UV Vis Spectrophotometer Specifications Optical Design Dual beam internal reference detector Wavelength Scanning Wavelength Data Interval 0 2 0 5 1 0 2 0 3 0 5 0 nm Photometric Measurement Modes Absorbance transmittance concentration lt 0 08 T at 220 nm and 340 nm lt 0 1 T at 340 nm and 400 nm lt 1 0 Photometric Stray Light YT at 198 nm Display Graphical with LCD backlight 9 7 x 7 1 cm 3 8 x 2 8 in Keypad Sealed membrane with tactile response keys n USB type A port for USB stick front panel USB type B port for computer rear panel USB type A port for printer rear panel 30 W x 40 D x25 H cm 11 8 x 15 7 x 9 8 in weight 8690988 Power Requirements 100 to 240 V 50 to 60 Hz Thermo Scientific Orion AquaMate Spectrophotometer User Manual 197 Chapter 11 Customer Services Orion AquaMate 7000 Vis Spectrophotometer Specifications Optical Design Single beam Tungsten halogen lamp 1000 hours Wavelength Scanning Speed 10 to 4 200 nm min Wavelength Data Interval 1 0 2 0 3 0 5 0 nm Photometric Measurement Modes Absorbance transmittance concentration 9 0 Photometric Stray Light o w nm and 340 nm lt 0 1 T at 340 nm and 400 nm lt 1 0 Display Graphical with LCD backlight 9 7 x 7 1 cm 3 8 x 2 8 in Keypad Sealed membrane with tactile response keys USB type A port
94. contents of one Silica HR Acid Rgt F10 Powder Pack straight from the foil into the same vial If silica or phosphate is present a yellow color will develop 9 Close the vial tightly with the cap and swirl or invert several times to mix the contents 10 Wait for a reaction period of 10 minutes 11 Add the contents of one Silica Citric Acid F10 Powder Pack straight from the foil into the Same vial In this step any yellow color due to phosphate is removed 12 Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial 13 Wait for a reaction period of 2 minutes 14 Place the vial into the holder in the sample chamber and close the chamber door 15 Press the Measure Sample function Key to display the result in mg l silica Notes Occasionally water samples contain forms of silica which reacts very slowly with molybdate The nature of these forms is not known A pre treatment with sodium hydrogen carbonate and then with sulfuric acid will make these forms reactive to molybdate pre treatment is given in Standard Methods for the Examination of Water and Wastewater under Silica Digestion with Sodium Bicarbonate Substance Interference lron Large amounts interfere Phosphate Does not interfere at concentrations less than 50 mg l PO At 60 mg l PO the interference is approximately 2 At 75 mg l PO the interference is approximately 11 Sulfide Interferes a
95. ction key to display the result in mg l free copper Notes For determination of total copper a digestion is required Extremely acid water samples pH 2 or less must be adjusted between pH 4 and pH 6 before the reagent is added with 8 mol l potassium hydroxide solution KOH Accuracy is not affected by undissolved powder Interferences Cyanide CN Cyanide prevents full color development Add 0 2 ml formaldehyde to 10 ml water sample and wait for a reaction time of 4 minutes cyanide is masked After this perform test as described Multiply the result by 1 02 to correct the sample dilution by formaldehyde Silver Agt If turbidity remains and turns black silver interference is likely Add 10 drops of Saturated potassium chloride solution to 75 ml of water sample Filtrate through a fine filter Use 10 ml of the filtered water sample to perform test 76 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2098 Cyanuric Acid Tablet Test Melamine Method 0 160 mg l CyA 1 Load and run the AC2098 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door a
96. ctor use the numeric Keypad to enter the factor value and press the enter key Repeat for each wavelength and factor Multiwavelength Test Name 340 0 532 0 633 0 ee Test 5 Press the Run Test function key Taking Measurements with Multiwavelength Method Once the parameters have been set for the multiwavelength test 1 Press the Run Test function key 2 Prepare the blank and samples that will be measured 3 If Auto 6 or Auto 3 is selected for the Sample Positioner setting a b Place the blank and samples in the appropriate positions on the carousel Confirm that the blank and samples are in the appropriate positions on the carousel and press the enter key Once the blank and samples have been measured remove them from the carousel To measure additional samples press the Measure Sample function key and repeat steps 3a through 3c 4 If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting qd Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank has been measured remove it from the carousel Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Sample function key Repeat for each sample 5 When all of the samples have been measured press the Save Data function key and follow the onscreen prompts
97. ctrophotometer e Sample cells for color testing are made of glass Glass is suitable for testing in the visible wavelength range A round glass 24 mm or 25 mm cell gives satisfactory results For best accuracy and precision at color levels that are lower than 15 color units a rectangular glass 50 mm cell is recommended The 10 mm sample cell size is not recommended for color testing Turbidity Effect on Color Measurement When measuring true color turbidity must be removed since it can cause light scattering and increase the color value Turbidity may also affect the precision of the measurement Chart 1 demonstrates that filtering reduces the color reading the more turbid the sample the more Significant the effect Chart 2 shows that the filtered sample generally shows better precision especially for turbid samples Chart 1 Color of unfiltered and filtered turbid Chart 2 Precision of the Color measurement in sample by using NCASI 71 01 method at unfiltered and filtered turbid water sample as 465nm CV 1 400 mUnfitterd mFiltered B Unfilteredturbid gFilteredturbid 3 0 2 5 D O 2 0 2 1 5 s 1 0 4 0 5 E 6 L 0 0 KA KA KI K o v Ta Wa U Z Z cn cn D K K E Thermo Scientific Orion AquaMate Spectrophotometers for Color Measurement The Thermo Scientific Orion AquaMate AQ8000 UV Vis and AQ7000 Vis spectrophotometers have the required wavelengths to analyze for any c
98. d service representative to perform any service procedure you do not feel comfortable performing 178 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 10 Maintenance Routine Care Routine care for the spectrophotometer does not require a lot of time To help minimize maintenance time and increase the life and performance of the instrument please follow these guidelines e Always replace the dust cover when the instrument is not turned on to prevent dust trom accumulating in and on the instrument e Donotuse or store the instrument in a corrosive environment e Gently wipe the outside of the instrument including the keypad with a soft cloth to remove any dust or spills Water isopropyl alcohol and other common laboratory cleaning agents may be used if necessary e Always clean up spills as soon as they occur to prevent or minimize damage to the instrument If concentrated acids or bases or any hydrocarbon materials are spilled on the instrument clean up the affected area immediately Cleaning and Maintaining Vials and Cuvettes Carefully check the condition of the vials cuvettes and other cells used to measure samples If they are chipped cracked or scratched it is important to discard the damaged vials and replace them with new ones Ensuring your vials are clean both inside and out is important to the quality of your results for two reasons 1 contaminating material may abs
99. d to allow the user to lock or unlock the file Utility Low High Limits Enters the lowest and highest acceptable results outside of which the result is flagged as Low or High Adv A T C Std Curve Abs Ratio Abs Diff Kinetics 3 Pt Net some Bio Tests Math Accesses manipulation functions of the graph Scanning Measure Blank as function key Initiates measurement of the blank Measure Blank as test parameter Selects the frequency of zeroing the instrument as Once or Every Reading Kinetics Measurement Mode Selects the type of photometric data reported for a measurement Abs T Conc in A T C Kinetics Scanning Multiwavelength Measure Samples Initiates measurement of samples Max X Enters a maximum X value to manually rescale the graph Kinetics Scanning Max Y Enters a maximum Y value to manually rescale the graph Kinetics Scanning Min X Enters a minimum X value to manually rescale the graph Kinetics Scanning Min Y Enters a minimum Y value to manually rescale the graph Kinetics Scanning iexeC rsor Selects a cursor point in functions using more than one cursor setting Scan Area and Scan 3 Pt Net calculations in the graph Scanning Number of Matched Enters the number of vials or cuvettes that will be run in the Correction Program Cuvettes maximum of 5 204 Orion AquaMate Spectrophotometer User Manual Thermo Scientific
100. data press the Edit Graph function key before performing other functions on the data Use the Edit Graph menu to rescale graphical scan data determine peak height using a 3 point net equation perform calculations on the scanned data and label peaks and valleys g 4 29pm 15Apri5 SCAN_O1 BLANK Wave length 550 0 When working with tabular scan data press the Edit Data function key before performing other functions on the data Use the Edit Data menu to rescale tabular scan data Scanning 2 59pm 16AprisS Test Name SCAN_O1 BLANK Select Data Save Data Press f or to select Press ENTER ee2eocecocos ecocoecocos faset ine collected pe Rescaling Graphical Scan Data Modify the scale of the scan data plot automatically or manually When Auto Scale is selected the instrument scales the X and Y axes so all of the data appears on the plot When Manual is selected manually select specific minimum and maximum values for the axes 1 Inthe graphical display press the Edit Graph function key 2 Press the Edit Scale function key to modify the scale 3 Inthe Edit Scale menu 136 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 7 Wavelength Scanning Test Menu 2 17pm 16ApriS SCAN_O1 BLANK Wave length IDH 2 Baseline collected 16Apr15 a Press the Auto Scale function key to automatically change the scale of the scanned data and display the new graph The instrument adjusts the m
101. dit Units Delete File Deletes a test or data file from the Stored Tests Directory Utility Delete Name Deletes the entire name to allow a new entry Test Name and Edit Units peee Removes a wavelength and factor from the list Multiwavelength and Performance Verification Diluent Volume Enters the volume of diluent added before measurement Dilution Multiplier in some Bio Tests Dilution Multiplier Displays the factor used to correct for sample dilution Display Activity Indicates whether results should include protein concentration DNA e 260 Calculates the extinction coefficient DNA Factor Enter the factor to calculate DNA concentration DNA Bio Tests Edit Changes a wavelength or factor in the list Multiwavelength and Performance Verification Edit Curve Manipulates the graph Kinetics Edit Data Selects a portion of data in a table for recalculation of result Kinetics Scanning Edit Graph Manipulates the graph Scanning Edit Scale Changes the graph axis scales and view individual data points Scanning Enters a factor to convert a datum to a result acter Abs x Factor 1 Concentration Result Abs min x Factor 2 Kinetics Result Can be entered or calculated from concentration and absorbance in Adv A T C Factor 1 Enters a factor to convert a datum to a result Abs WL1 x Factor Result Abs Ratio Abs Difference Multiwavelength Factor 2 Enters a factor to convert a datum to a re
102. e 9 200 mg l Clo Load and run the AC3072 method Filla clean AQUAfast 16 mm round vial Cat No AC2V16 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one Chlorine HR KI Tablet straight from the foil into the vial Crush the tablet with a clean stir rod Add one Acidifying GP Tablet straight from the foil into the same vial Crush the tablet with a Clean stir rod Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l chlorine Notes Oxidizing agents interfere as they react in the same way as chlorine 68 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2099 Chlorine Dioxide Tablet Test DPD Method 0 02 11 mg l C10 Chlorine Dioxide Measurement in Absence of Chlorine L 2 ee ee Load and run the AC209924 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly w
103. e the escape of chlorine dioxide gases i e by pipetting or shaking must be avoided The analysis must take place immediately after taking the sample The DPD color development is carried out at a pH value of 6 2 to 6 5 The reagent tablet therefore contains a buffer for the pH adjustment Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the tablet is added use 0 5 mol l sulfuric acid or 1 mol l sodium hydroxide Exceeding the measuring range Concentrations above 19 mg l chlorine dioxide can lead to results showing 0 mg l In this event the water sample must be diluted with water free of chlorine dioxide 10 ml of the diluted sample should be mixed with the reagent and the measurement repeated Oxidizing agents such as chlorine or ozone interfere as they react in the same way as chlorine dioxide 70 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate CODLO00 COD Low Range Digestion Tube Test Dichromate Digestion Method 0 150 mg l 02 l Open one 16 mm COD reaction vial and add 2 ml of deionized water this is the reagent blank vial 2 Open a second 16 mm reaction vial and add 2 ml of sample this is the sample vial 3 Close the vials tightly with the caps and gently invert the vials several times to mix the contents CAUTION The vials will become hot during mixing 4 Heat
104. e Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 9 Performance Verification Performance Verification 12 36pm 6Ju115 Stray Light filters Wavelength Expected Measured Result 220 0nm O 1 T O 1 T O 1 T Place SRE filters in position i 3 Press Start Test CO ae 2 Test 5 Press the Start Test function key 6 The results will indicate pass or fail for each wavelength If the test fails follow these guidelines e Repeat the test twice to verify that the test is failing consistently e Make sure all the filters being used are specifically designed to measure stray light at the Specific wavelengths e Verify that the filters are placed in the correct positions e Contact technical support for more troubleshooting advice Orion AquaMate Spectrophotometer User Manual 177 Maintenance The spectrophotometer is durable and reliable so routine maintenance is minimal This section explains e Routine Care e Changing the Fuse e Replacing the Tungsten Lamp Orion AquaMate 7000 Vis instruments only Warning Operating the instrument with the cover off exposes the operator to potentially dangerous voltages and ultraviolet UV radiation Therefore we recommend that only authorized service representatives perform procedures requiring removal of the instrument cover and replacement of electrical components To protect both yourself and the instrument be sure to contact an authorize
105. e appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Sample function key Repeat for each sample 5 When all of the samples have been measured press the Save Data function key and follow the onscreen prompts to save the sample measurements to the USB memory stick Recalling an Existing Advanced A T C Method 1 Press the test key to access the Test Types menu 2 Press the Aor W key to highlight Advanced A T C and press the enter key Test Types 2 12pm 21AprisS Water Analusis Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Net Multiwavelength Performance Verification Advanced A xT C Test Name Measurement Mode Wave length Ref Wavelength Correction Delay Time min sec sample Positioner Humber of Samples More parameters Press f or to select item to change De See Test Tests Test 4 Press the A or Y key to highlight Load internal test or Load test from USB drive and press the enter key 5 Press the A or Y key to highlight the desired test and press the enter key Thermo Scientific Orion AquaMate Spectrophotometer User Manual 153 Chapter 8 Absorbance Transmittance and Concentration Measurements Multiwavelength The Multiwavelength application obtains multiple fixed wavelength measurements Itis a fast alternative to scanning if the wavelengths of interest are well known T
106. e chamber door 10 Press the Measure Blank function key to measure the blank 11 Open the sample chamber door Remove the blank vial from the holder 12 Place the sample vial into the holder in the sample chamber and close the chamber door 13 Press the Measure Sample function key to display the result in mg l manganese Notes Rinse all glassware with 1 1 nitric acid solution first and then rinse with deionized water Water samples that contain more than 300 mg l CaCO hardness after adding the ascorbic acid powder pack add 10 drops of Rochelle salt solution After addition of the alkaline cyanide reagent solution a cloudy or turbid solution may form in some water samples The turbidity should disappear after the PAN indicator solution is added Water samples containing more than 5 mg l iron should be allowed to react for at least 10 minutes 86 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P55 Manganese High Range Powder Pack Test Periodate Oxidation Method 0 1 18 mg l Mn High Range 1 Load and run the AC4P55 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the
107. e function key use the numeric keypad to enter the minute value and press the enter key When using the am pm time format press the Set AM PM function key until the correct setting is shown Thermo Scientific Thermo Scientific Chapter 3 Orion AquaMate Instrument Setup Standby Setting To prolong the life of the lamp the spectrophotometer is pre set at the factory to automatically go into standby mode after 15 30 minutes of inactivity To change this setting press the A or V key to highlight the Standby option press the enter key press the A or Y key to highlight the desired standby setting and press the enter key to save the setting Utility 2 04pm 25Mari5 Software Revision Instrument Serial Number Printe Date T Standby Mode OFF Go into Standby Mode now Bazeli Go into Standby Mode in 15 min Keeper SG eee el eg ee ee Langua Go into Standby Mode in 60 min z of 1 Go into Standby Mode in 9O min Screen Printo Press f or to select Stored Press ENTER USE Dr Calculator T or to select parametar Baseline Expiration Setting If be performing scans on samples a time limit can be set for which a collected baseline will be valid This is particularly useful when measurements are made in a production setting across multiple shifts or when the nature of the blank sample changes dramatically with time To set the baseline expiration time press the A or W key to highlight the Baseline Expiration
108. easuring Samples Setting the Wavelength 1 Press the Set nm function key to set the wavelength value 2 Use the numeric keypad to enter the wavelength value for taking measurements Absorbance 4 05pm a oi 0 0968 oB Oni Enter Wavelength 190 0 1100 0 3 Press the Set nm function key again to save the entered wavelength value Measuring a Blank 1 Place the blank in the vial holder carousel Note If a 3 position or 6 position vial holder carousel Is in use the blank must be placed in the B position The instrument always uses the B position to collect the blank measurement 2 To enter an absorbance or transmittance value for the blank use the numeric keypad to enter the desired value 3 Press the Measure Blank function key Measuring Samples 1 Prepare the samples to be measured 2 Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location 3 The absorbance ABS or percent transmittance T measurement will be shown on the instrument display Orion AquaMate Spectrophotometer User Manual 143 Chapter 8 Absorbance Transmittance and Concentration Measurements Using the Advanced A T C Test Method Use the Advanced A T C application for absorbance or transmittance measurements that include a measurement delay time or for automating the measurement of multiple samples with a cell changer This section covers
109. ected in the utility menu Sample changer keys Press to select the location of the vial to be measured B is the blank position and 1 5 are the sample positions when using the Auto 6 sample positioner setting and B is the blank position and 2 and 4 are the sample positions when using the Auto 3 sample positioner setting 14 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 2 Spectrophotometer Basics Vial Holder Carousels The Orion AquaMate 8000 and Orion AquaMate 7000 instruments include the three position vial holder carousel preinstalled in the sample compartment The three position carousel has one adjustable 13 to 25 mm round vial holder in position B one 10 mm square vial holder in position 2 and one 20 to 50 mm long path rectangular vial holder in position 4 The Orion AquaMate 8000 instrument also includes the six position vial holder carousel and the one position vial holder carousel for 10 mm square vials and traditional spectrophotometer applications allowing you to take advantage of the full capabilities of the instrument AquaMate Three Position Carousel Catalog Number AQ003C Side View Top View AquaMate Six Position Carousel AquaMate One Position Carousel Catalog Number AQO06C Catalog Number AQOO1C A new one position carousel that holds one 100 mm rectangular vial can now be purchased using Catalog Number AQ100C Warning If the Sample Positioner optio
110. ected Result aiu persmsei08 was Pierence Evaluation Absorbance 0 358 cm 0 360 cm 0 002 cm1 0 6 Good Organic Carbon Concentration 25 0 mg L 24 9 mg L 0 01 mg L 0 4 Bias readings of a KHP organic carbon standard at 25 0 mg L in phosphate buffer prepared per SM 5910B tested in a 1 cm cell demonstrate good accuracy e The average AQ8000 absorbance result is within 0 002 absorbance units of the average reading expected per SM 5910B 0 6 difference from the expected absorbance e The average AQ8000 organic carbon concentration result calculated per SM 5910B is within 0 1 mg L of the expected value 0 4 difference 99 6 recovery from the expected value of 25 0 mg L organic carbon 120 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Precision of Samples Maximum RSD Method UV254_1 per SM 5910B dorian heated Evaluation Absorbance 14 lt 10 7 RSD 0 3 RSD Good Precision readings of a KHP organic carbon standard at 25 0 mg L in phosphate buffer prepared per SM 5910B tested in a 1 cm cell demonstrate good precision e The relative standard deviation RSD of 14 test results on the AQ8000 Is 0 3 RSD well within the maximum 10 7 limit expected per SM 5910B Notes 1 Ifthe preprogrammed method is not on the memory stick download the method file from the our Online Library at www thermoscientifi
111. een repeated readings The Measure Blank parameter is used to select the frequency of zeroing the instrument as function key as once or every reading See the Parameters section for more information on the test parameter settings Press the A or Y key to highlight More parameters and press the enter key to view additional parameters displayed on the second page tilen oJulis nk Display Result Rate Factor More parameters Press f or to select item to change Test Tests Test Kinetics Factor Units Linearity Value sample Positioner IDH CO 0FF Low High Limits 999979999 Auto Print i Auto Save Data Off More parameters Press f or to select item to change ee Se Test Tests Test 4 Press the Save Test function key and follow the onscreen prompts to save the test Taking Measurements with Kinetics Method Once the parameters have been set for the kinetics test 1 Press the Run Test function key 2 Prepare the blank and sample that will be measured 164 Orion AquaMate Spectrophotometer User Manual Thermo Scientific 3 4 Chapter 8 Absorbance Transmittance and Concentration Measurements If Auto 6 or Auto 3 is selected for the Sample Positioner setting a b Place the blank and sample in the appropriate positions on the carousel Confirm that the blank and sample are in the appropriate positions on the carousel and press the enter key Once the blank and s
112. emove the digestion vials from the reactor and allow them to cool to room temperature Open the cooled digestion vials and add the contents of one TN Reagent A Power Pack Straight from the foil into each vial Use a funnel to add the reagent Close the vials tightly with the caps and shake the vials for at least 15 seconds to mix contents Wait for a reaction period of 3 minutes Open the digestion vials and add the contents of one TN Reagent B Power Pack straight from the foil into each vial Use a funnel to add the reagent Close the vials tightly with the caps and shake the vials for at least 15 seconds to mix contents The reagent will not completely dissolve Wait for a reaction period of 2 minutes Open two TN Acid LR HR Reagent C Vials and add 2 ml of the digested treated blank to the first vial this is the blank vial Add 2 ml of the digested treated sample to the second vial this is the sample vial Close the vials tightly with the caps and gently invert the vials at least 10 times to mix contents Hold the vial in a vertical position with the cap pointing up Turn the vial upside down Wait for all of the solution to flow down to the cap Return the vial to the upright position Wait for all the solution to flow to the bottom of the vial This process is one inversion 10 inversions equal about 30 seconds Wipe the exteriors of the vials CAUTION The vials will become warm during mixing Wait for a reaction period o
113. ength If the test fails follow these guidelines e Repeat the test twice to verify that the test is failing consistently e Make sure the target and tolerance values you entered for the calibrated wavelength are the same as the values on the calibration certificate for the standard 172 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 9 Performance Verification Photometric Accuracy This test measures the absorbance or T of a set of standards and compares the results with specified tolerances The wavelength absorbencies and tolerances are preset but you should change them to the values on the calibration certificate included with your standards Note You can display the tolerances for this test in either absorbance or Transmittance When running the Photometric Accuracy test remember e You need photometric accuracy standards calibrated to known absorbance values at specified wavelengths e Measure the standards in the order they are listed on the test screen e Youcan use one to five standards To run the Photometric Accuracy test 1 Press the test key to access the Test Types menu 2 Press the Aor W key to highlight Performance Verification and press the enter key 3 Press the A or V key to highlight Photometric Accuracy and press the enter key Performance Verification 12 16pm 6Ju115 Photometric Accuracy H nm Expected Tol T xT zT Result A 1 590 0 50
114. ept 0 037 Corr Coeff 9 99E 01 Page 1 Standards 1 3 Mos T ER eel ES Graph Test Standards Test qd b C d Press the View Graph function key to display a graph of the standard curve data rve 11 52am i15ApriS CHLOR INE_CURVE BLANK 0 051 mg L Curve Fit 5 lope 0 531 Std Dev 8 S6E 03 Intercept 0 037 Corr Coeff 9 99E 01 Tabular To save the test with the standard curve press the Save Test function key and follow the onscreen prompts Press the Edit Standards function key to edit the standard curve Press the Run Test function key to start measuring samples Measuring Samples using a Standard Curve Once the standards have been measured for the standard curve Press the Run Test function key Prepare the blank and samples that will be measured If Auto 6 or Auto 3 is selected for the Sample Positioner setting qd b 126 Orion AquaMate Spectrophotometer User Manual Place the blank and samples in the appropriate positions on the vial holder carousel and press the Measure Samples function Confirm that the blank and samples are in the appropriate positions and press the enter key to begin taking measurements Thermo Scientific Chapter 6 Standard Curve Test Menu Standard Curve 1 43pm 15AprisS Test Name CHLORINE_01 Cell g Insert blank into position E Insert samples i 2 into positions 2 amp 4 Press ENTER to measure 4 If Manual 6 or Single Cell Holder is se
115. er User Manual Thermo Scientific Chapter 11 Customer Services 11 Wait while the USB driver software is located and installed 12 Approve any security messages by clicking the Install button 13 Once the installation of the USB driver software is complete click the Close button 14 Record the COM port number of the Spectrophotometer USB connection in the Device Manager window File Action Yew Help eee mm ol ee oo ae G 3 fatteries J Muetooth Radios amp Computer i ControlVault Device toa Disk drives i Display adapters iy DVD CD ROM drives 25 Human Interface Devices Sg JOE ATAATAFI controllers F IEEE 1394 Bus host controllers Keyhoards P Mice and other pointing devices a Modems L Monitors W Network adapters a i Ports COM amp LPT T ECP Printer Port LPT1 MI Spectrophotomerer COM J Processors g Smart cand readers Example Data Collection Program The AquaMate communcation settings are as follows e Bits persecond 19200 e Data bits 8 e Parity None e Stop bits 1 e Flow control None 1 Selecta computer program for data collection and make sure the program including device drivers and plug ins is fully installed Realterm data collection software can be downloaded from their website and is used in the following instructions as an example 2 Open the data collection software program by clicking the Realterm icon on the computer desktop 3 Click the Port tab on the RealTerm window
116. ey 3 Use the name entry screen to delete an existing custom unit delete a character in the name of a unit add a character to the name of a unit or accept the name of a unit Orion AquaMate Spectrophotometer User Manual 207 Appendix A General Instrument Information a To enter anew custom unit press the A or W key to highlight a character press the Add Character function key and repeat until the desired name is shown Press the Accept Name function key to save the entered name Edit Unit 11 49am 41Apri5 HBCDEFGH I JKLMNOPQRS TUYWX Y20123456789 abcdefghijkilmnopgrstuvuxygz _ pH Press f or to select character l l Ade 4 Press the A or Y key to highlight the custom concentration unit and press the enter key Advanced A xT C 11 49am 41Apri5s Ref Wavelengt Delay Time mi Conc of Standa Factor nits sample Positio Humber of Samp More paraneter Press f or to select Press ENTER Press f or 4 t O ee se O CUnitd 208 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Appendix A General Instrument Information Calculations for Software e Caleulaton Equation Standard Curves Partial Sums SX Xa SEDI SXX x SYY SXY gt xii SQX X N SXX SX SQY X0 7 N SYY SY SSXY XG x yi Y N SXY SX SY Where xi Concentration of i standard yi Absorbance of ih standard N Number of standards
117. ey Press the A or Y key to highlight Load test from USB drive and press the enter key Press the A or Y key to highlight the test method and press the Load Test function key Press the Run Test function key Running a Test Method from the AquaMate Instrument Library Press the test key to access the Test Types menu Press the A or Y key to highlight Water Analysis and press the enter key Press the Stored Tests function key Press the A or Y key to highlight Load internal test and press the enter key Press the A or Y key to highlight the test method and press the Load Test function key Press the Run Test function key Running a Test Method from the AquaMate SmartStart Menu L 2 3 4 Press the test key to access the Test Types menu Press the SmartStart function key Press the A or Y key to highlight the test method and press the Load Test function key Press the Run Test function key Orion AquaMate Spectrophotometer User Manual 37 Chapter 4 Water Analysis Test Menu Running a Water Analysis Test Method 1 When a preprogrammed test method has been loaded the following screen will appear Water Analysis Bi 6MariS AC4SP71 Saved 1 bzorbance 510 0nnm OFF OFF Manual 3 Auto Print Auto Save Data More parameters Press f or to select item to change es ee Test Tests Test Water Analysis Test Name AC4P71 Result Bik a 3 Open the sample compartment door If the desired sample
118. f 5 minutes Load and run the ACD004 method Place the blank vial into the holder in the sample chamber and close the chamber door 92 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate 19 Press the Measure Blank function key to measure the blank 20 Open the sample chamber door Remove the blank vial from the holder 21 Place the sample vial into the holder in the sample chamber and close the chamber door 22 Press the Measure Sample function key to display the result in mg l nitrogen Notes Appropriate safety precautions and a good lab technique should be used during the whole procedure Volumes for samples and blank should always be measured using 2 ml volumetric pipettes class A One blank is sufficient for each set of samples After taking the blank measurement it is possible to measure several Samples It is very important to remove the vials from the reactor after exactly 30 minutes Large quantities of nitrogen free organic compounds that are included in some water Samples may reduce the effectiveness of the digestion by reacting with the persulfate reagent Samples that are well Known to contain large quantities of organic compounds must be diluted and digestion and measurement must be repeated for checking the effectiveness of the digestion Application for water wastewater and seawater In
119. f this user manual Visit www thermoscientific com water to view Thermo Scientific Orion products and download product literature user manuals and manuals software updates and additional application and technical resources For the most current warranty information refer to the Thermo Scientific Orion warranty card included on the Thermo Scientific Orion AquaMate literature CD and available online at www thermoscientific com water 186 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 11 Customer Services External Device Interfacing and Data Transfer The following instructions describe how to interface an Orion AquaMate spectrophotometer with an external device transfer the data and save the data as a Microsoft Excel Word or Notepad file The operator must set the Orion AquaMate spectrophotometer settings to allow data to be sent to an external device install the AquaMate USB driver on the computer and install a computer program for a data collection program such as Realterm or HyperTerminal AquaMate Spectrophotometer Setup for Computer Interfacing 1 Power on the Orion AquaMate spectrophotometer 2 Press the utility key to acess the utility menu 3 Press the A or Y key to highlight the Printer option and press the enter key to open the printer menu Utility 11 55am 43Juni4 software Revision 1 004 Instrument Serial Number W2KR091104 Printer OFF Date Time Setup 11 55a
120. foil into the Sample in the beaker Dissolve the powder using a clean stirring rod 5 Wait fora reaction period of 30 seconds 6 Add the contents of one Hexamine F20 Powder Pack straight from the foil into the same Sample in the beaker Dissolve the powder using a clean stirring rod 7 Add 1 drop of Aluminum ECR Masking Reagent into the vial marked as blank Add 10 ml of the prepared sample to the same vial this is the blank vial 8 Add the remaining 10 ml of the prepared sample to the second vial this is the sample vial 9 Close the vials tightly with the caps and swirl or invert several times to mix the contents Wipe the exteriors of the vials 10 Wait for a reaction period of 5 minutes 11 Place the blank vial into the holder in the sample chamber and close the chamber door 12 Press the Measure Blank function key to measure the blank 13 Open the sample chamber door Remove the blank vial from the holder 14 Place the sample vial into the holder in the sample chamber and close the chamber door 15 Press the Measure Sample function key to display the result in mg l aluminum Notes Before use clean the vials and the measuring beaker with hydrochloric acid approximately 20 Rinse them thoroughly with deionized water To get accurate results the sample temperature must be between 20 C and 25 C A low test result may be given in the presence of fluorides and polyphosphates The effect of this is generally insignif
121. for USB stick front panel USB type B port for computer rear panel USB type A port for printer rear panel 30 W x 40 D x 25 H cm 11 8 x 15 7 x 9 8 in Wei 8690988 Power Requirements 100 to 240 V 50 to 60 Hz Connectivity Note We reserve the right to make product improvements and updates Specifications are Subject to change without notice 198 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 11 Customer Services Ordering Information Cat No Description AquaMate 8000 UV Vis spectrophotometer with methods on USB stick 6 position turret 3 position turret 1 position turret user documentation dust cover USB cable and 110V 220V 240V power cords AquaMate 7000 Vis Spectrophotometer with methods on USB stick 3 position turret user documentation dust cover USB cable and 110V 220V 240V power cords AquaMate 6 position vial holder turret for six 10 mm square vials AquaMate 3 position vial holder turret for one adjustable 13 25 mm round vial one adjustable 20 50 mm rectangular vial and one 10 mm square vial AquaMate 1 position vial holder turret for one 10 mm square vial COD165 Thermoreactor for digestion methods 100 120 150 160 165 C temperature control CODSO01 1000 ppm COD standard 475 mL CODS10 10000 ppm COD standard 475 mL Alkalinity M acid indicator method tablet reagent 100 tests AquaMate 1 position vial holder turret for one 100mm rectangular vial
122. g a Test e Recalling and Recalculating Graphical Kinetics Results e Rescaling and Recalculating Tabular Kinetics Results e Modifying the scale of the plot You can work with graphical or tabular data and perform the same functions with either However the location of the function keys depends on the display type Note The Kinetics application can measure only one sample ata time Note The Kinetics application collects up to 400 data points per run When setting test parameters select the interval time and total run time accordingly Setting the Parameters for Kinetics Method 1 Press the test key to access the Test Types menu 2 Press the A or VY key to highlight Kinetics and press the enter key Test Types 16pm 63u115 Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Scanning 3 Point Met Multiuave length Performance Verification ee T Start Tests ATC Thermo Scientific Orion AquaMate Spectrophotometer User Manual 163 Chapter 8 Absorbance Transmittance and Concentration Measurements 3 Highlight and change the displayed test parameters including Test Name Measurement Mode Wavelength Interval Time Total Run Time Factor Units Linearity Value and Sample Positioner qd The Delay Time parameter is used to enter the time from test initiation to first measurement allowing for sample equilibration The Interval Time parameter is used to enter the time betw
123. g the vial clean is very important for long vial life e Never store vials or cuvettes long term in a water or solvent bath between uses If the solvent you are using dries impurities in the water or solvent may be deposited on the inside of the vial or cuvette causing permanent damage e Use only lens cleaning tissue paper or fine soft cloth to wipe optical surfaces Most paper products such as facial tissues paper towels etc contain wood fibers that can damage the vial or cuvette material e Atthe end of the day ensure that all vials or cuvettes are well cleaned and stored in a Suitable container after drying Term Definition Dilute acid Dilute nitric acid lt 10 Acid Hydrochloric 5M acid or nitric acid 5M see the Note below Solvent rinse Rinse with the solvent that was originally used to solvate your analyte Copious water rinse Use a pure water deionized distilled RO and rinse at least 10 times Use a neutral pH detergent Triton X 100 if available to dilute acid wash water Detergent 9 rinse to remove residue Note Do not use 5M nitric acid on an anti reflection mirror coated vials or cuvettes 180 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 10 Maintenance Important Using an ultrasonic cleaning bath for your vials or cuvettes is not recommended Each bath generates a different frequency therefore if your bath operates at the resonant freq
124. gh Zero 5 lope 0 626 Std Dev 1 94E 02 ave ange ALL Tabular 130 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 6 Standard Curve Test Menu Recalling an Existing Standard Curve Method Press the test key to access the Test Types menu Press the A or Y key to highlight Standard Curve and press the enter key Test Types 11 07am 47 MaridS Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Met Multiwavelength Performance Verification Press f or to select es E T E Start Tests ATC 3 To recall an existing standard curve press the Stored Tests function key Standard Curve 9 54am eo Test Name Date Standards Measured Wave length Ref Wavelength Correction Curve Fit Humber of Samples More parameters Press T or to select item to change Standards Test_ Tests Press the A or W key to highlight Load internal test or Load test from USB drive and press the enter key Press the A or Y key to highlight the desired test and press the enter key Utility Test Directory 2 25pm 15AprisS Press f or to select test Test Test Test Orion AquaMate Spectrophotometer User Manual 131 Wavelength Scanning Test Menu The Wavelength Scanning test technique lets you measure the absorption or percent transmission spectrum of a sample You can use scans to determine peak wavelengths or to
125. gning the lamp assembly Captive screw on lamp door Thumbscrew _ Tab on cable connector Q Q 4 Raise the lamp door 184 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 10 Maintenance Unscrew the thumbscrew while holding the lamp assembly in place Carefully pull the assembly out until you can reach the connector Release the tab and disconnect the cable Unpack the new lamp assembly Use a Soft lint free cloth to wipe off any fingerprints on the lamp Connect the cable to the connector Ld 12 13 14 15 16 Position the new assembly as shown above making sure to align the pins correctly Tighten the thumbscrew Close the lamp door Turn on the instrument and press the utility key Press the arrow keys to highlight Lamp Hours and press the enter key Press the enter key to reset the lamp hours to zero Orion AquaMate Spectrophotometer User Manual 185 Customer Services Technical Support For any questions or if you require assistance contact our Technical Support Specialists e Email wai techservbev thermofisher com e Within the United States call 1 800 225 1480 e Outside the United States call 1 978 232 6000 or fax 1 978 232 6031 For additional product information contact your local authorized dealer Thermo Scientific Orion technical sales representative or contact us using the Water and Laboratory Products WLP information on the page back o
126. has been added The temperature of the sample is important for full color development At a temperature below 20 C the reaction period is 15 minutes Conversion mg l NH mg I N x 1 29 mg l NH3 mog I N x 1 22 Orion AquaMate Spectrophotometer User Manual 53 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P12 Ammonia Powder Pack Test Salicylate Method 0 01 0 8 mg I N Ammonia as Nitrogen Load and run the AC4P12 method Use two clean AQUAfast 24mm round vials Cat No AC2V24 Pour 10 ml of deionized water into the first vial this is the blank vial Pour 10 ml of sample into the second vial this is the sample vial oa A WO N FF Add contents of one Ammonia Salicylate F10 Powder Pack straight from the foil into each vial Close the vials tightly with the caps and swirl or invert several times to mix Wait for a reaction period of 3 minutes 7 Add contents of one Ammonia Cyanurate F10 Powder Pack straight from the foil into each vial Close the vials tightly with the caps and swirl or invert several times to mix Wipe the exteriors of the vials 8 Waitfora reaction period of 15 minutes 9 Place the blank vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Blank function key to measure the blank 11 Open the sample chamber door Remove the blank vial from the holder 12 Place the sample vial into the holder in the sample chamber and close
127. he Measure Blank function key to measure the blank 20 Open the sample chamber door Remove the blank vial from the holder 21 Place the sample vial into the holder in the sample chamber and close the chamber door 22 Press the Measure Sample function key to display the result in mg l nitrogen Notes Appropriate safety precautions and a good lab technique should be used during the whole procedure Volumes for samples and blank should always be measured using 2 ml volumetric pipettes class A One blank is sufficient for each set of samples After taking the blank measurement it is possible to measure several samples It is very Important to remove the vials from the reactor after exactly 30 minutes Large quantities of nitrogen free organic compounds that are included in some water Samples may reduce the effectiveness of the digestion by reacting with the persulfate reagent Samples that are well Known to contain large quantities of organic compounds must be diluted and digestion and measurement must be repeated for checking the effectiveness of the digestion Application for water wastewater and seawater Interfering substances that resulted in a concentration change of 10 Bromide more than 60 mg l and chloride more than 1000 mg l produce positive interferences Orion AquaMate Spectrophotometer User Manual 95 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC3048 Ozone Tablet Test DP
128. he outer walls of the vials are dry and clean before performing the analysis Fingerprints or water droplets on the light entry surfaces of the vials lead to incorrect measurements e Blank and measurement procedures should be performed using the same vial whenever possible since different vials can possess slightly different tolerances e Always take all readings with capped vials e Bubbles on the inside walls of the vial can lead to incorrect measurements To prevent this cap the vial and remove the bubbles by swirling the vial before performing the test e Always add the reagent to the sample straight from the foil The reagent should never touch fingers or hands e Major temperature differentials between the instrument and environment can lead to incorrect measurements i e due to the formation of condensate in the area of the lens or on the vial Specified tolerances at T 20 C e Forthe best results use a pipette to measure and add samples to vials or beakers 48 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2002 Alkalinity M Alkalinity to pH 4 3 Tablet Test Acid Indicator Method ha L 2 Pr PY a 8 9 200 mg l CaCO Load and run the AC2002 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the
129. he sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add exactly 2 ml SPADNS Solution to the vial CAUTION The vial will be filled to the top 7 Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial 8 Place the vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Rgnt Blank function key to measure the reagent blank 10 Open the sample chamber door and remove the vial from the holder 11 Empty the vial thoroughly rinse the vial and cap several times and then fill the vial with exactly 10 ml of sample 12 Add exactly 2 ml SPADNS Solution to the vial CAUTION The vial will be filled to the top 13 Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial 14 Place the vial into the holder in the sample chamber and close the chamber door 15 Press the Measure Sample function key to display the result in mg l fluoride Notes Reverse color methods use a reagent that when prepared with samples deceases in color as the concentration of the species being measured in the samples increases Reverse color methods require the use of both a blank and a reagent blank The blank is a clear Solution deionized water with zero absorbance The reagent bl
130. he sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one gram 1 g Hydrazine Powder to the vial Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial Wait for a reaction period of 10 minutes The slight turbidity that occurs when the reagent is added must be removed by filtration 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l hydrazine Notes Thermo Scientific lf the water sample is cloudy you must filter it before performing the blank measurement The temperature of the water sample should not exceed 21 C Using the hydrazine spoon 1 g is equivalent to one level spoon Qualitative folded filter papers for medium precipitates are recommended In order to check whether the reagent has aged if ithas been stored for a lengthy period perform the test as described above using tap water If the result is above the detection limit of 0 05 mg l you should only use the reagent with reservations as there may be a major deviation in results Orion AquaMate Spectrophotometer User Manual 81 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC 2078 Iron Il amp Ill Tablet Test PPST Method 0 02
131. hemist Allen Hazen The color is a light yellow to brown color The range is 0 to 500 Platinum Color Units PCU Intermediate standards are prepared from a platinum cobalt 500 ppm stock solution that is available commercially or can be prepared by usert A unit of color is the color produced by 1 mg L platinum in the form of chloroplatinum ion The color values measured by comparison with the platinum cobalt standards can be expressed as PCU Pt Co APHA or Hazen units depending on the specific procedures In the United States the National Secondary Drinking Water Regulation for color is a maximum of 15 color units The World Health Organization recommends that drinking water color does not to exceed 15 true color units 114 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Other Color Standards and Color Units For Samples where the color is different from Pt Co standards several different standards and color scales are used such as ADMI Color American Dye Manufacturer s Institute Gardner Color Saybolt Rosin EBC European Brewery Convention CIE System etc Platinum Cobalt Color Methods Two single wavelength methods are commonly used in the United States to measure water and wastewater color with color characteristics similar to that of the Pt Co standards e Water Wastewater at 455 nm This method utilizes a spectropho
132. his section covers e Setting Up Test Parameters and Adding Wavelengths and Factors e Taking Measurements e Recalling a Test Setting the Parameters for Multiwavelength Method 1 Press the test key to access the Test Types menu 2 Press the Aor Y key to highlight Multiwavelength and press the enter key Test Types Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Net ultiwvave length Performance Verification ee Start Tests ATC 3 Highlight and change the displayed test parameters including Test Name Measurement Mode and Sample Positioner a Some parameters only appear when the concentration mode Is selected while others 4 154 Orion AquaMate Spectrophotometer User Manual appear regardless of the selected measurement mode See the Parameters section for more information on the test parameter settings Hu ltiwavelength 1 lepa Julis IDH O 0FF Auto Print Auto Save Data Press f or to select item to change ave Dred Set nms Sest Tee Press the Set nms function key to add the wavelengths and factors Factors can only be added when the measurement mode is setto Conc F actor Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements Press the Add nm function key use the numeric keypad to enter the wavelength value and press the enter key lf the measurement mode is set to Conc Fa
133. holder is not in the beam press the appropriate Sample Changer key on the instrument keypad corresponding to the correct vial holder position B for the adjustable round vial holder 2 for 10 mm square vials and 4 for 20 50 mm rectangular long path vials If necessary the instrument will rotate the carousel to place the correct vial holder in the measurement position 4 Inserta vial containing the blank or zero solution into the sample holder Note Ideally the same vial should be used or one that has been matched to the sample vial 5 Close the lid and press the Measure Blank function key The instrument will set the measurement wavelength and perform a blank measurement 38 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 4 Water Analysis Test Menu Water Analysis Test Name AC4P71 Result Blank S Sampo 6 Open the lid and remove the vial containing the blank or zero solution 7 Place the vial containing the sample into the sample holder and close the lid 8 Press the Measure Sample function key The results will displayed as shown below Water Analysis Test Name AC4P71 Result Page 1 Samples 1 3 Maar Dae Blank Data sample 9 To save the measurements to the USB memory stick press the Save Data function key Note The blank measurement will be remembered as long as you are working in the sample test method After pressing the Run Test function
134. hr min option press the enter key use the numeric keypad to enter the time value and press the enter key to save the setting Utility 2 lipm 25Mari5 Software Revision 1 004 Instrument Serial Number W24K091104 Printer PC Date Time Setup Standb Keeper Language of lamp life used screen Contrast Printout Contrast Stored Tests Directory Entry Enter time 00 10 24 00 O OFF Orion AquaMate Spectrophotometer User Manual 25 Chapter 3 Orion AquaMate Instrument Setup Beeper Setting The instrument will emit an audible beep each time a key is pressed when the beeper option Is on To change this setting press the A or W key to highlight the Beeper option and press the enter key to toggle the setting between Off and On Screen Contrast Setting Adjust the screen contrast on the instrument to make it easier to read the display To set the screen contrast press the A or W key to highlight the Screen Contrast option press the enter key press the function key or function key to adjust the contrast and press the esc key to save the setting Utility 2 2 pm 25Mari5 Software Hevision 1 004 Instrument Serial Number W2R091104 Printer PC Date Time Setup 2 2epm 5 Mar 15 Standby 30 mi Baseline Expiration Chrimind Press t for darker contrast Press for lighter contrast Press ESC to exit Calculator Function To use the calculator function press the A or W key to highlight the Calculator option a
135. ia High Range Reaction Tube Test Salicylate Method 1 50 mg l N Ammonia as Nitrogen l 2 3 4 1 8 9 Load and run the ACR011 method Open one 16 mm reaction vial and add 0 1 ml of deionized water this is the blank vial Open a second 16 mm reaction vial and add 0 1 ml of sample this is the sample vial Add the contents of one Ammonia Salicylate F5 Powder Pack straight from the foil into each vial Add the contents of one Ammonia Cyanurate F5 Powder Pack straight from the foil into each vial Close the vials tightly with the caps and swirl or invert several times to mix the contents Wipe the exteriors of the vials Wait for a reaction period of 20 minutes Place the blank vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank 10 Open the sample chamber door Remove the blank vial from the holder 11 Place the sample vial into the holder in the sample chamber and close the chamber door 12 Press the Measure Sample function key to display the result in mg l ammonia as N Notes Strong alkaline or acidic water samples must be adjusted to approximately pH 7 before analysis use 1 mol l hydrochloric acid or 1 mol l sodium hydroxide If chlorine is known to be present add one drop of 0 1 mol l sodium thiosulfate for each 0 3 mg l Cl in a one liter water sample Iron interferes with the test The interferences can be eliminated as foll
136. ial Use a funnel to add the reagent 13 Close the vial tightly with the cap and swirl the vial for 10 15 seconds to mix the contents The reagent will not completely dissolve Wipe the exterior of the vial 14 Wait for a reaction period of 2 minutes 15 Place the vial into the holder in the sample chamber and close the chamber door 16 Press the Measure Sample function key to display the result in mg l total phosphate as phosphorus P Notes Appropriate safety precautions and good lab technique should be used during the whole procedure Ortho phosphate ions react with the reagent to form an intense blue color Phosphate in organic and condensed inorganic forms meta pyro and polyphosphates must be converted to ortho phosphate ions before analysis Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed Inorganic forms Organically combined phosphates are converted to ortho phosphate ions by heating with 104 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate acid and persulfate The amount of organically combined phosphates can be calculated mg l phosphate organic mg l phosphate total mg l phosphate acid hydrolysable e Application for water wastewater and seawater e Highly buffered samples or samples with extreme pH values should be adjusted between pH 2 and pH 10 before analy
137. ical support for more troubleshooting advice Orion AquaMate Spectrophotometer User Manual 175 Chapter 9 Performance Verification Stray Light This test measures the stray light at selected wavelengths and compares the measurements with expected values The wavelengths and expected values are preset and cannot be changed Running the stray light test takes about 30 seconds When running the stray light test remember Stray light standards are required that are designed to measure stray light at 220 nm UV Vis only 340 nm and 400 nm 1 e must have 0 1 T at the wavelength of interest For UV Vis instruments e Position B should be empty e Use position 1 for the 220 nm stray light standard SRE 220 or equivalent e Use position 2 for the 340 nm stray light standard SRE 340 or equivalent e Use position 3 for the 400 nm stray light standard SRE 400 or equivalent For Vis only instruments e Position B should be empty e Use position 1 for the 340 nm stray light standard SRE 340 or equivalent e Use position 2 for the 400 nm stray light standard SRE 400 or equivalent To run the Stray Light test Press the test key to access the Test Types menu Press the A or Y key to highlight Performance Verification and press the enter key Press the A or Y key to highlight Stray Light and press the enter key In the Stray Light screen make sure the wavelengths and tolerances are set correctly 176 Orion AquaMat
138. icant unless the water has fluoride added artificially 52 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2012 Ammonia Tablet Test Indophenole Blue Method 0 02 1 mg l N Ammonia as Nitrogen l 2 Pr PE a 9 Load and run the AC2012 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one Ammonia No 1 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod Add one Ammonia No 2 Tablet straight from the foil into the same sample in the vial Crush the tablet with a clean stir rod Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial Wait for a reaction period of 10 minutes 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l ammonia as N Notes Thermo Scientific The tablets must be added in the correct sequence The Ammonia No 1 tablet will only dissolve completely after the Ammonia No 2 tablet
139. icles are dissolved After cooling down the sample is set to a pH value of 3 to 6 by using ammonia solution Refill with deionized water to the previous volume of 100 ml and mix well 10 ml of this pre treated solution is used for analysis perform as described by the selected test method ll Water that has been treated with organic compounds like corrosion inhibitors must be oxidized as necessary to break down the iron Therefore add 1 ml concentrated Sulfuric acid and 1 ml concentrated nitric acid to 100 ml water sample and boil to approximately half volume After cooling down proceed as described above 82 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P78 iron Ferro Powder Pack Test 1 10 Phenanthroline Method 0 02 3 mg l Fe 1 Load and run the AC4P78 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add the contents of one Ferro F10 Powder Pack straight from the foil into the vial 7 Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial 8
140. iltered to remove suspended materials such as algae and particulates which cause turbidity True color is the result of only dissolved species in water natural organic matter minerals or chemicals Techniques to Measure Color in Water There are two main approaches to measuring water color e Visual methods a water sample is visually compared to a series of colored standards e Spectrophotometric methods the color is determined by measuring how much light is absorbed or transmitted through a sample at a single wavelength or at a number of certain wavelengths The results are then compared with a known color standard or used in various algorithms which are defined by the test method Many groups publish methods for measuring color Examples of spectrophotometric methods are Platinum Cobalt Color Tristimulus Values and ADMI methods Specific method references for color in water include APHA Standard Methods 2120 EPA 110 1 DIN ISO 7887 and SAC 436 nm China MEP GB 11903 NCASI Technical Bulletin 253 or 803 and others Color Standards and Color Units Platinum Cobalt Color Standard and Units The most common color measurement of water and wastewater is Platinum Cobalt Pt Co color also known as APHA color or Hazen color These names are commonly used in different applications but they are based on identical procedures Measurement with the Pt Co APHA Hazen standard is based on the Hazen color scale that was introduced in 1892 by c
141. in mg l total chlorine Notes Alternatively the AC207210 method can be used with 10mm square vials and the AC207250 method can be used with 50mm rectangular vials All blank and sample volumes must remain the same as those specified in these instructions so Samples may need to be prepared in separate containers and then transferred into the selected vial Vial cleaning As many household cleaners i e dishwasher detergent contain reducing Substances the subsequent determination of chlorine may show lower results To avoid any measurement errors only use glassware free of chlorine demand Preparation Put all applicable glassware into sodium hypochlorite solution 0 1 g l for one hour and then rinse all glassware thoroughly with deionized water For individual testing of free and total chlorine the use of different sets of glassware Is recommended EN ISO 7393 2 5 3 Preparing the sample When preparing the sample the escape of chlorine gases i e by pipetting or shaking must be avoided The analysis must take place immediately after taking the sample 64 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate The DPD color development is carried out at a pH value of 6 2 to 6 5 The reagents therefore contain a buffer for the pH adjustment Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 bef
142. inimum and maximum values for the X and Y axes so all the data appears on the plot b Press the Manual function key to manually change the scale of the scanned data values and display the new graph Press the Min Y Max Y Min X or Max X function key use the numeric keypad to enter the new value and press the enter key to apply the new value The instrument will redraw the graph using the entered minimum and maximum values c Press the Cursor function key to identify specific points along the X axis Press the lt Cursor function key to move to the desired minimum wavelength value and press the Set Min X reset the minimum wavelength value and redraw the graph Press the Cursor function key to move to the desired maximum wavelength value and press the Set Max X reset the maximum wavelength value and redraw the graph Scanning 1 59pm 16AprisS Test Name SCAN_O1 BLANK 0 370 ILON Wave length 550 0 Wave length Abs 500 0 0 305 Performing Calculations on the Scan Data Modify the scan data graph by performing calculations on the data 1 Inthe graphical display press the Edit Graph function key 2 Press the Math function key to modify the graph by performing calculations on the data 3 In the Math menu Thermo Scientific Orion AquaMate Spectrophotometer User Manual 137 Chapter 7 Wavelength Scanning Test Menu 2 20pm 16ApriS SCAN_O1 BLANK IDH gee OQ Wave length 550 0 li cp are ESE riS IDEF C o Fe
143. ion of the new standard and press the enter Key Follow the steps in the Measuring Standards for a Standard Curve section to re measure all of the standards c If Delete Standard is selected the highlighted standard will be deleted d IfClear Measurements is selected all absorbance measurements will be removed from the standard curve data e IfResetStandards is selected all concentration and absorbance measurements will be removed from the standard curve data Orion AquaMate Spectrophotometer User Manual 129 Chapter 6 Standard Curve Test Menu Selecting a Different Curve Fit for a Standard Curve To change the curve fit for a standard curve the standard curve must be displayed as a graph not as a table 1 With the standard curve displayed as a graph press the Change Fit function key 2 26pm 15ApriS mg L Std Dev i Intercept 0 034 Corr Coeff 1 00E 00 Tabular 2 Press the A or Y key to highlight the desired curve fit and press the enter key Standard Curve 2 29pm 15AprisS Test Name CHLORINE_CURVE BLANK 0 357 Linear Through Zero 2nd Order 3rd Order 4th Order Segmented Press f or to select Press ENTER mg L Std Dev Intercept 0 034 Corr Coeff 1 00E 00 a a 3 The instrument will apply the selected curve fit to the data and display the new fit Standard Curve 2 40pm 15AprisS Test Name CHLORINE_CURVE LANK 0 357 T TT TT TT TT TTT TT mg L 0 593 Curve Fit Linear Throu
144. ith samples deceases in color as the concentration of the species being measured in the samples increases Reverse color methods require the use of both a blank and a reagent blank The blank is a clear Solution deionized water with zero absorbance The reagent blank is a mixture of the reagent and sample with no EDTA reagent and provides a zero concentration point with the darkest color highest absorbance The color of samples prepared with the EDTA reagent will decrease as the concentration increases for this method Measuring the reagent blank needs to be done with each sample analysis The tablets must be added in the correct sequence In the case of high levels of residual chlorine perform the analysis with a dechlorinated water sample Orion AquaMate Spectrophotometer User Manual 113 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Color Measurement from Application Log 131 Color Measurement Water Color The color of our water is important not only for drinking purposes but also for aquatic environments and for home and industrial uses Color in water can be caused by dissolved and Suspended materials In water and wastewater color measurement there Is a distinction between apparent color and true color Apparent color is the color of the sample as received and includes color due to both the dissolved and suspended materials in the water True color is the color in the sample after it has been f
145. ith the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Empty the vial leaving a few drops of sample remaining in the vial Add one DPD No 1 Tablet straight from the foil into the vial Crush the tablet with a clean Stir rod Add sample to the 10 ml mark on the vial Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l chlorine dioxide Chlorine Dioxide Measurement in Presence of Chlorine L 2 eS eS a Load and run the AC209924 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Empty the vial leaving a few drops of sample remaining in the vial Add one DPD No 1 Tablet straight from the foil into the vial Crush the tablet with a clean Stir rod Filla second clean AQUAfast 24mm round vial with
146. ition key Close the lid then press the Measure Blank key 3 If required testan SCS Using the same cell empty and fill with the prepared SCS wipe dry and insert into the sample holder Close the lid and press the Measure Sample key Record the displayed result 4 The reading for the SCS should be within the desired criteria per your QA plan See Results section for examples Sample Storage Samples are not preserved Analyze as soon as possible after collection Samples may be Stored for up to 48 hours at lt 6 C prior to analysis See EPA Method 415 3 for SUVA storage Sample Preparation pH adjust and or sample filtration For non SUVA If the pH is not between 4 and 10 adjust pH per steps in oH Adjustment of Sample p 2 Note Do not adjust pH for a SUVA determination For UVA UV254 Set up the filtration apparatus with a 0 45 um filter Wash the filter with 50 mL DI and discard the rinse water Filter 50 mL of the sample Test the filtrate Thermo Scientific Orion AquaMate Spectrophotometer User Manual 119 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Sample Measurement Ensure the instrument has been zeroed properly Touching only the frosted sides of the cell rinse the clean cell with a portion of the filtered sample then fill with the filtered sample Wipe dry Insert the sample cell into the holder close the lid and press the Measure Sample key Record the displayed result
147. k T CSU cwecstcassnsiolsanatnsdasaatseavencseeantgaianatnantaatianamatietein 84 AC2055 Manganese Tablet Test s ssssessssresesrsrnrnnnnrnrnnnnrnrnnrnnnnnrnnnnnnrnrennrnrennrrnrenrnns 85 AC4P54 Manganese Low Range Powder Pack amp Liquid TeSt 86 AC4P55 Manganese High Range Powder Pack TeSt ccssecssssrssssrsssserssssererseeeen 87 AC4P 42 Molybdate Powder Pack TeSt csssssssrsrsssssrssesrsrssesrerssssrsesseersessersereen 88 ACR007 Nitrate Reaction Tube Test s sssssssssissssresrresnsresnnrnnnnrnnnnnnnnrnnnsrrnsrnresnrresrnrenns 89 AC2046 Nitrite Tablet TeSt ssssssssssrsrsrsrnrnrnnnnnnnnrnrnrnunurnnnrnnnnnnnnnnrnrnnnnnnnnnnrnrnrnnrnrnrernrnrrerns 90 AC4P 46 Nitrite Powder Pack Test ussssssssirrsrsisnrssnnsrsrsrnansnnnnrsrsrnansrrsrnannrsnnisrarernnrinrana 91 ACD004 Nitrogen Total Low Range Digestion Tube TeSt c cscs 92 ACDO007 Nitrogen Total High Range Digestion Tube T St ccsssscsssssessereeees 94 AC3048 OZONE Tapet eS Decanter n tus alasmmiwwieidee lau ieamabatvienien alata 96 AC 2001 DF Tabet S Kusnia a naa unncmommRntaNs 98 je 10K aim COUI TeS a et ere mtet tent rene merece ern N 99 AC2095 WA Phosphate Ortho Low Range Tablet TeSt 100 AC2096 Phosphate Ortho High Range Tablet TeSt 101 AC4P 95 Phosphate Ortho Powder Pack T St csccsssscesssrssssesserssererssetersssetteess 102 ACR095 Phosphate Ortho Reaction Tube TeSt ccssssssssscssssssssssssrssssrssserssersetes 103 ACD095 Phosphate
148. key 4 While the instrument is taking the baseline a Collecting baseline message will be shown indicating the progress of the baseline scan After the baseline is measured the message will update to Baseline Collected Remove the blank from the sample compartment g 4 18pm 15Apri5 SCAN_O1 BLANK Wave length 550 0 IDH 1 Baseline collected 15Apri5 5 Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location 6 Press the Measure Sample function key 7 When the scan is complete a graph of the scanned measurement will be displayed Press the Tabular Graph function key to switch between tabular and graphical displays 4 21pm ao 450 0 Wave length 550 0 IDH 1 Baseline collected i5Apri5 Enis tee ES Ean Baseline i sample 134 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 7 Wavelength Scanning Test Menu Scanning 4 24pm 15ApriS Test Name SCAN_O1 BLANK 0 0 0 0 IDH 1 Baseline collected i5Apri5 Ene com ES ES Baseline Data Sample Thermo Scientific Orion AquaMate Spectrophotometer User Manual 135 Chapter 7 Wavelength Scanning Test Menu Viewing and Manipulating Scanned Data The Scanning test technique allows results to be viewed and manipulated in graphical or tabular form When working with graphical scan
149. key the Measure Sample function key will be shown if the blank has already been measured and pressing the Measure Sample function key will use the previously blank measurement The blank measurement will be cleared automatically if any test method settings are changed if the test method is saved or if a new test method is loaded Note When running a reverse color test method a reagent blank measurement will be required after the measurement of the standard blank Insert the vial containing the reagent blank and press the Measure Rgnt Blank function key Open the lid and remove the reagent blank See the Using the Reverse Color Feature section for detailed instructions Note If the Statistics option is set to Off in the test parameters menu no Average or Std Dev information will be displayed in the measurement screen Orion AquaMate Spectrophotometer User Manual 39 Chapter 4 Water Analysis Test Menu Using the One Point Adjustment Feature Use the one point adjustment feature prior to running a preprogrammed test method to ensure accurate measurements This procedure is recommended each time a new batch of reagents are used to account for variations in batch to batch reagent composition and other factors that affect the accuracy of a method with a fixed calibration curve When the one point adjustment feature is off no adjustment is applied when running the test and test results are calculated exactly according to the preprogrammed e
150. l into the holder in the sample chamber and close the chamber door Press the Measure Rgnt Blank function key Open the sample chamber door and remove the vial from the holder Wipe the exterior of the standard vial and place the standard vial into the holder in the sample chamber and close the chamber door Press the Measure Standard function key Open the sample chamber door and remove the vial from the holder The display will show the entered standard concentration the measured standard concentration without a correction factor and the calculated adjustment correction factor Hater Analysis 9 59am 27MariS Test Mame AC4P71 BLANK Standard Concentration 1 000 mg L Measured Concentration 1 260 mg L Adjust Correction Blank fecert standard Press the Accept function key if the calculated adjustment correction factor is acceptable Typically a correction factor of 0 7 to 1 3 within 30 Is acceptable To save the adjustment correction factor to the test method press the Save Test function key and overwrite the existing test method or save as a new test method Press the Run Test function key When running a test with an adjustment correction factor in use the display will show 1pt Adj next to the test name Water Analysis 10 02am 4 7MariS Test Name AC4SP71iCipt Adj BLANK Result Blank Orion AquaMate Spectrophotometer User Manual 41 Chapter 4 Water Analysis Test Menu Using the Reverse Color Feature
151. lected for the Sample Positioner setting a Place the blank in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location b Press the Measure Blank function key Once the blank has been measured remove it from the carousel c Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Sample function key to measure the sample Repeat for each sample Standard Curve 1 29pm 15AprisS Test Name CHLORINE_CURVE BLANK bs Result Fage 1 Samples 1 3 ese ee Sisk Se Blank Data Samp le 5 When all of the samples have been measured press the Save Data function key and follow the onscreen prompts to save the sample measurements to the USB memory stick Do not use more than eight characters when naming the saved data file The data file will be saved as a TXT file in the Thermo folder on the USB memory stick Thermo Scientific Orion AquaMate Spectrophotometer User Manual 127 Chapter 6 Standard Curve Test Menu DATA_04LTXT Notepad Standard Curve 1 35pm 15Apri15 Test Name CHLORINE_CURVE Saved Date Standards Measured 15Apr15 Wave length 510 Onm Ref Wavelength Correction otf Curve Fit Linear Number of standards Units mg L Sample Positioner Manual 6 ID 0 0FF off Low High Limits 99997 9999 Statistics otf Auto Print ott Auto Save Data ott
152. let are dissolved chloride causes an extremely fine distributed turbidity with a milky appearance Heavy shaking leads to bigger sized particles that can cause false readings High concentrations of electrolytes and organic compounds have different effects on the precipitation reaction lons that also form deposits with silver nitrate in acidic media such as bromides iodides and thiocyanates interfere with the analysis Highly alkaline water should if necessary be neutralized using nitric acid before analysis Orion AquaMate Spectrophotometer User Manual 59 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2070 Chlorine Free amp Total Tablet Test DPD Method 0 01 6 mg l Cl L 2 O p O e 10 11 12 13 14 15 16 17 Load and run the AC207024 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Empty the vial leaving a few drops of sample remaining in the vial Add one DPD No 1 Tablet straight from the foil into the vial Crush the tablet with a clean Stir rod Add sample to the 10 ml mark on the vial Close the vial tightly with the cap and swirl or invert seve
153. let is dissolved Wipe the exterior of the vial 11 Wait for a reaction period of 1 minute 12 Place the vial into the holder in the sample chamber and close the chamber door 13 Press the Measure Sample function key to display the result in mg l silica Notes The tablets must be added in the correct sequence 108 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2061 Silica with Phosphate Removal Tablet Test Silicomolybdate Method with Phosphate Removal 0 05 4 mg l SiO 1 Load and run the AC2061 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Silica No 1 Tablet straight from the foil into the vial Crush the tablet with a clean Stir rod 7 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved 8 Wait for a reaction period of 5 minutes 9 Add one Silica PR Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 10 Add one Silica No 2 Tablet straight from the foil into the same vial Crush the tablet with a Clean Sti
154. low illustrates the position of the light beam in the instrument Beam size specifications are shown below e Distance from bottom of the vial cuvette to center of beam Z dimension 8 5 mm e Beam dimensions 2 mm wide by 7 mm tall 16 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Six Position Carousel One Position Carousel Chapter 2 Spectrophotometer Basics Changing the Vial Holder Carousel The Orion AquaMate 8000 and Orion AquaMate 7000 instruments have the three position vial holder carousel preinstalled in the sample compartment This carousel can be changed to use the six position vial holder carousel or one position vial holder carousel Installing the Six Position or One Position Vial Holder Carousel 1 Open the sample compartment door and let it rest on its hinge 2 Remove the preinstalled three position vial holder carousel Loosen the captive thumbscrew and pull straight up on the carousel to lift it out of the sample compartment 3 With one hand carefully lower the six position or one position carousel straight down into the sample compartment Captive Thumbscrew Captive Thumbscrews Alignment Pin Holes 4 With the other hand tighten the captive thumbscrew or thumbscrews 5 Close the sample compartment door Note If the carousel is not aligned correctly you will not be able to tighten the thumbscrews Orion AquaMate Spectrophotometer User Manu
155. m 43Juni4 Standby i Baseline Expiration Chrimind Keeper Language OF lamp life used screen Contrast Printout Contrast Stored Tests Directory USB Drive Files Calculator Press f or to select 4 Press the A or Y key to highlight the PC option and press the enter key to set the PC Option to On Utility 11 56am 43Juni4 Software Revision Instrument Serial Number Internal PC External Press f or to select Press ENTER to change Press ESC to exit USE Drive Files Calculator Press f or to select parametar 5 Press the esc key until the instrument returns to the main measurement screen Orion AquaMate Spectrophotometer User Manual 187 Chapter 11 Customer Services USB Computer Cable Interfacing and USB Driver Installation 1 Connect the USB computer cable to the USB communication port on the back of the AquaMate spectrophotometer USB communication port 2 Insert the Orion AquaMate Documentation DVD into the computer CD DVD drive 3 Connect the USB computer cable to a USB port on the computer 4 Open the computer Device Manager a Click the Start icon b Right click the Computer button c Click the Properties button d Click the Device Manager button 5 Double click the Spectrophotometer button on the Device Manager window Dy Device Manager Sox File Action View Help 9 m 6 8 m eRe Display adapters 43 DVD CD ROM drives OF Human Interface Devi
156. mance Verification Noise Measurement This test measures the amount of noise The Vis instrument measures only at 340 nm All test parameters are determined by instrument specifications and cannot be changed by the user When running the noise test remember e Perform the 0A measurement with the vial holder empty Optionally you can perform the 2A measurement with a 2A filter To run the Noise Measurement test 1 Press the test key to access the Test Types menu 2 Press the Aor Y key to highlight Performance Verification and press the enter key 3 Press the A or W key to highlight Noise Measurement and press the enter key Performance Verification 12 29pm 6Ju115 Noise Measurement POA Peak to Peak Meas Result Tolerance 230 lt 0 002 340 z0 001 e7A Peak to Peak Meas Result Tolerance 230 0nm 0 003 340 0nm 0 002 Make sure the Blank position is empty Place 2A filter in Cell Position 1 Press Start Test Test 4 With the Blank position empty insert the 2A filter in position 1 Ignore the test results at 2A if you do not have a filter installed in position 1 5 Press the Start Test function key 6 The results will indicate pass or fail for each wavelength If the test fails follow these guidelines e Repeat the test twice to verify that the test is failing consistently e Make sure the instrument is warmed up for at least 30 minutes with the standby mode feature turned off e Contact techn
157. mber AQ006C 3 position vial holder carousel Catalog Number AQ003C 1 position vial holder carousel Catalog Number AQ001C AquaMate user documentation CD Printed instrument test report Dust cover USB cable 110V US apan power cord Catalog Number AQ110V 220V Euro power cord Catalog Number AQ220V 240 V UK Singapore power cord Catalog Number AQ 240V Orion AquaMate 7000 Vis Spectrophotometer Vis Spectrophotometer Preprogrammed Methods on USB memory stick Catalog Number AQOUSB 3 position vial holder carousel Catalog Number AQ003C AquaMate user documentation CD Printed instrument test report Dust cover USB cable 110V US apan power cord Catalog Number AQ110V 220V Euro power cord Catalog Number AQ220V 240 V UK Singapore power cord Catalog Number AQ240V Note The China Australia New Zealand power cord Catalog Number AQCHNV must be ordered separately Orion AquaMate Spectrophotometer User Manual 9 Chapter 1 Spectrophotometer Introduction Orion AquaMate User Documentation CD Orion AquaMate Spectrophotometer Site and Safety Getting Started User Guide Declaration of Conformity Master Method List Warranty AQUAfast Reagent Instructions Requires Adobe Reader Th you do not have Adobe Reader you can download a 2013 by Thermo Fisher Soentfic Inc Al rights reserved free copy of Adobe Reader software directly from Adobe s website Adobe and Reader are trademarks of Adobe Systems Incorpora
158. meter User Manual 83 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P79 Iron Total Powder Pack Test TPTZ Method 0 02 1 8 mg l Fe mn FP WwW N FF 6 1 8 9 Load and run the AC4P 79 method Use two clean AQUAfast 24mm round vials Cat No AC2V24 Pour 10 ml of deionized water into the first vial this is the blank vial Pour 10 ml of sample into the second vial this is the sample vial Add the contents of one Iron TPTZ F10 Powder Pack straight from the foil into each vial Close the vials tightly with the caps and swirl or invert several times to mix the contents Wipe the exteriors of the vials Wait for a reaction period of 3 minutes Place the blank vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door Remove the blank vial from the holder 10 Place the sample vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l iron Notes For determination of total iron digestion is required TPTZ reagent recovers most insoluble iron oxides without digestion Rinse all glassware with 1 1 hydrochloric acid solution first and then rinse with deionized water to remove iron deposits that can cause slightly high results Strong alkaline or acidic water samples must be adjusted between pH 3 and pH 8 bef
159. meters Press f or to select item to change ia Gee Sees Standard Test Tests Alphanumeric Entry Highlight the desired parameter i e Test Name and press the enter key The Create Test Name window will open Press the A or V key to highlight a character press the Add Character function key and repeat until the desired name is shown Press the Accept Name function key to save the entered name Create Test Mame 1 19pm 25Mari5 ABCDEFGH I JKLMNOPORS TUVUX YZ20112 3456789 abcdefghijkilmnopqrstuvuxgz _ pCIt Test Mame Copper_01 Press f or to select character Hame Character Character Hame Orion AquaMate Spectrophotometer User Manual 21 Chapter 3 Orion AquaMate Instrument Setup setting Utility Menu Options Use the Utility menu to view the current software revision instrument serial number and remaining lamp life to set instrument parameters including printer computer interface date and time standby baseline expiration Keypad beeper language and screen contrast and to access a directory of all stored tests USB drive files and calculator function The utility parameters cannot be set or changed when the instrument is performing a measurement To access the Utility menu press the utility key on the instrument keypad Utility 1 20pm 25Mari5 Software Revision 1 004 Instrument Serial Number W2R091104 Printer OFf Date Time Setup Stored Tests Directory USB Drive Files Calculator T or
160. mg l greater than 100 mg l greater than 10 mg l greater than 10 mg l Sulfide greater than 100 mg l greater than 80 mg l Orion AquaMate Spectrophotometer User Manual 103 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ACD095 Phosphate Total Digestion Tube Test Persulfate Digestion Ascorbic Acid Method 0 02 1 1 mg l P Phosphate as Phosphorous 1 Open one 16 mm PO4 P Acid Reagent Digestion Tube and add 5 ml of sample 2 Add the contents of one Potassium Persulfate F10 Power Pack straight from the foil into the vial Use a funnel to add the reagent 3 Close the vial tightly with the cap and invert the vial several times to mix the contents 4 Heat the vial for 30 minutes in the preheated reactor at a temperature of 100 C 5 CAUTION The vial will be hot Remove the vial from the reactor and allow It to cool to room temperature 6 Open the cooled digestion vial and add 2 ml of 1 54 N Sodium Hydroxide Solution to the vial 7 Close the vial tightly with the cap and gently invert the vial several times to mix the contents Wipe the exterior of the vial 8 Load and run the ACD095 method 9 Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Blank function key to measure the blank 11 Open the sample chamber door Remove the vial from the holder 12 Add the contents of one Phosphate Rgt F10 Power Pack straight from the foil into the v
161. n Tube Test Dichromate Digestion Method 0 1500 mg l 0 1 Open one 16 mm COD reaction vial and add 2 ml of deionized water this is the blank vial 2 Open a second 16mm COD reaction vial and add 2 ml of sample this is the sample vial 3 Close the vials tightly with the caps and gently invert the vials several times to mix the contents CAUTION The vials will become hot during mixing 4 Heat the vials for 120 minutes in the preheated reactor at a temperature of 150 C 5 CAUTION The vials will be hot Remove the vials from the reactor and allow them to cool to 60 C or less Gently invert the vials several times to mix the contents while still warm Allow the vials to cool to room temperature before measuring Wipe the exteriors of the vials 6 Load and run the CODHOO method 7 Place the blank vial into the holder in the sample chamber and close the chamber door 8 Press the Measure Blank function key to measure the blank 9 Open the sample chamber door Remove the blank vial from the holder 10 Place the sample vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l oxygen Notes Run samples and blanks with the same batch of vials The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch Do not place the hot vials in the sample chamber Cool the vials to room temperature for
162. n is set to Auto6 whenever the Run Test function key is pressed to start a measurement the instrument will initialize the sample positioner and then run the blank in the B location on the sample positioner and measure five samples in the 1 5 locations on the sample positioner Orion AquaMate Spectrophotometer User Manual 15 Chapter 2 Spectrophotometer Basics Selecting and Positioning Vials and Cuvettes The compatible wavelength range for different types of vials and cuvettes depends on the material used The path length of test tubes is not as well defined as that of square cuvettes Vial Cuvette Type Wavelength Range Optical Glass 360 nm to gt 1100 nm Borosilicate Glass 330 nm to gt 1100 nm Quartz 190 nm to gt 1100 nm Disposable Polystyrene gt 340 nm Methacrylate gt 300 nm Acrylic gt 280 nm UV transparent gt 220 nm Note See the manufacturer s specifications and work within the recommended range Position vials and cuvettes so that the clear sides face the light beam one clear side facing the front of the instrument and the other facing the back Note Always place vials in the instrument in exactly the same orientation in the light beam An alignment mark on the vial helps orientation the vial consistently and correctly When using small aperture small volume cuvettes e Always used cuvettes with black masking e Use the same cuvette for your blank and your samples Z dimension The figure be
163. n of results in graphical or tabular form When the results are displayed the range start and stop time can be modified and the instrument recalculates the reaction rate When working with tabular kinetics results press the Edit Data function key before you can rescale and recalculate After collecting kinetics data you can use all the data for the rate calculation or select specific Start and end times When you modify the start and end times or select all the data the instrument recalculates and displays the new reaction rate and result The edit screen allows you to use all the data to recalculate the results and select specific start and end times for the rate calculation and recalculate the results To use all the data to calculate the reaction rate 1 With tabular kinetics data displayed on the Edit Data screen press the Use All Data function key 2 The instrument will calculate and display the rate To select specific start and end times for the rate calculation 1 With tabular kinetics data displayed on the Edit Data screen press the A or W key to highlight the appropriate data point in the table 2 Press the Start Time or End Time function key to display the recalculated rate and result Orion AquaMate Spectrophotometer User Manual 167 Performance Verification Performance Verification Tests Thermo Scientific Orion AquaMate 8000 UV Vis and AquaMate 7000 Vis spectrophotometers offer performance verification tests
164. n or results may be used with a corresponding total organic carbon TOC result to calculate the Specific UV Absorbance SUVA value for a water sample See Reference 1 for details References 1 EPA Method 415 3 Rev 1 1 UV254 for SUVA htto www epa gov microbes ordmeth htm 2 Standard Methods 5910B UV Absorbing Organic Constituents www standardmethods org 3 Orion AquaMate 8000 UV Vis Instrument User Guide Recommended Equipment e Thermo Scientific Orion AquaMate AQ8000 spectrophotometer e Filtration apparatus e g Fisher XX1004707 0 45 um filters 47 mm e g Fisher HNWP 04700 e Vacuum source aspirator air flow or water flow hand operated or low pressure electric vacuum pump e UV disposable cuvettes 1 cm Fisher 14 377 009 or quartz sample cells e Carousel for 10 cm cell if needed Orion AQ100C e Orion pH meter and electrode Solutions 1 Organic free deionized water DI 2 Reagents for pH adjustment Sodium hydroxide 0 1N Hydrochloric acid 0 1N Orion pH 4 01 and 7 00 Buffers Orion 910104 Orion 910107 3 Spectrophotometer Check Solution SCS optional Organic Carbon KHP in pH 7 phosphate buffer see Reference 2 for preparation or Commercial SCS PN 222 234700 www unitylabservices com Sample Cell Storage and Cleaning In order to obtain reproducible results clean and store sample cells per instructions in the Orion AquaMate Instrument User Guide 118 Orion AquaMate Spectrophotometer User
165. n the vial holder carousel If needed use the Sample Positioner keypad to set the vial location The concentration measurement will be shown on the instrument display Thermo Scientific Orion AquaMate Spectrophotometer User Manual 149 Chapter 8 Absorbance Transmittance and Concentration Measurements Using the Advanced A T C Test Method Use the Advanced A T C mode to obtain advanced concentration measurements Methods data and factors can be saved in this application This advanced concentration mode is useful for very simple comparisons that do not require a standard curve For better accuracy use the Standard Curve Test Technique The Advanced A T C mode measures concentration using either a standard or factor to convert absorbance readings to concentration units When using a standard specify the concentration of the standard and measure its absorbance When using a factor specify the factor and concentration units The steps for taking measurements in the two modes are similar the only difference is if a standard is measured or a factor is entered Setting the Parameters for Advanced A T C Method 1 Press the test key to access the Test Types menu 2 Press the Aor Y key to highlight Advanced A T C and press the enter key Test Types 2 12pm 21AprisS Water Analysis Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Net Multiwavelength Performance Verification Stant Teste
166. nal reagents or samples create new methods using Calibration standards or update methods using published wavelengths and equations Use a variety of vial sizes with the included 3 position vial holder carousel and update the vial position on the carousel with a Key press the sample changer keypad moves the carousel to the desired location for easy vial switching Performance verification tests ensure wavelength accuracy and instrument functionality plus built in filters allow for wavelength verification with no additional equipment required Additional functions include standard curve concentration measurements wavelength scanning multiple fixed wavelength measurements absorbance ratio and difference One year instrument warranty The Orion AquaMate 8000 UV Vis spectrophotometer measures in the 190 nm to 1100 nm wavelength range using a Xenon Flash lamp that requires no warm up time and has a five year average lifespan The Orion AquaMate 7000 Vis spectrophotometer measures in the 325 to 1100 nm wavelength range using a Tungsten lamp designed for easy replacement using the factory pre aligned lamp and base 8 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 1 Spectrophotometer Introduction Packing List Orion AquaMate 8000 UV Vis Spectrophotometer UV Vis Spectrophotometer Preprogrammed Methods on USB memory stick Catalog Number AQOUSB 6 position vial holder carousel Catalog Nu
167. nd press the enter key Use the numeric keypad to enter the first value Press the function key function key x function key or function key Use the numeric keypad to enter the second value Press the enter key to perform the calculation Utility 2 43pm 25Mari5 Software R Calculator 26 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 3 Orion AquaMate Instrument Setup Managing Stored Tests The instrument uses test method files that contain the parameters and settings needed to run the test including those for the Sample Positioner Setting After setting the test parameters assign a unique test name and save the test to either the internal instrument memory or USB memory stick so the test can be recalled and run without having to set the parameters again The instrument can save approximately 90 tests to its internal memory Tests saved to the internal memory will be maintained by battery backup when the instrument is powered off To access the Test Types menu press the test key on the instrument keypad The list of all available test techniques will be shown Use the following instructions as a general guide when setting up various test methods Test Types 10 35am 46Mari5S Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Met Multiwavelength Performance Verification a E T E Start Tests ATC Naming a
168. nd Saving a Test When naming a test enter the name using 8 to 16 alphanumeric characters The selected test type will determine the maximum number of alphanumeric characters for the test name The instrument will emit an audible beep when the maximum number of characters has been entered When saving a test itcan be saved to an external memory device using the front USB port or saved to the instrument s internal memory 1 After setting the test parameters highlight Test Name and press the enter key Water Analysis 10 47am 46Mari5 Test Name Measurement Mode Wave length ipt Adjust Reverse Color sample Positioner IDH O 0FF Statistics Auto Print Auto Save Data More parameters Press f or to select item to change a n Test Tests Test Orion AquaMate Spectrophotometer User Manual 27 Chapter 3 Orion AquaMate Instrument Setup 2 Inthe Create Test Name screen use the function keys to delete the test name delete a character in the name or add characters to the name and then accept the entered name Create Test Mame 11 21am 246Mari5 ABCDEFGH I JKLMNOPQRS TUYWX Y20123456789 abcdefghijkilmnopqrstuvuxgz _ pCIF Test Name TEST_12345 Press T or to select character l l Tard A a To delete the entire displayed test name press the Delete Name function key and to delete individual characters press the Delete Character function key b To enter characters press the A or W key to highlight the desired ch
169. nd remove the vial from the holder 6 Add one Cyanuric Acid Tablet straight from the foil into the vial Crush the tablet with a Clean stir rod 7 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved See notes below Wipe the exterior of the vial 8 Place the vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Sample function key to display the result in mg l cyanuric acid Notes Thermo Scientific If cyanuric acid is present a cloudy solution will occur Small single particles are not necessarily caused by cyanuric acid Dissolve the tablet completely swirl the vial for approximately 1 minute Undissolved particles of the tablet can cause results that are too high Exceeding the measurement range samples with concentration above 90 mg l must be diluted with water free of cyanuric acid 10 ml of the diluted sample should be tested as described above and the displayed results calculated using the dilution factor Orion AquaMate Spectrophotometer User Manual 77 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2009 Fluoride SPADNS Liquid Test SPADNS Method 0 05 2 mg l F 1 Load and run the AC2009 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with exactly 10 ml of deionized water Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in t
170. nd suitable for use as wavelength standards When running the internal standard test remember that e The wavelengths and tolerance values are preset and cannot be changed e The vial holder should be empty To run the Wavelength Accuracy Internal test Press the test key to access the Test Types menu Press the A or Y key to highlight Performance Verification and press the enter key Press the A or Y key to highlight Wavelength Accuracy Internal and press the enter key Press the Start Test function key Once the test is complete the results will indicate pass or fail for each wavelength Performance Verification 11 52am 6Ju115 Wavelength Accuracy Internal Tolerance Measured Result Pass Pass Pass Make sure the Blank position is empty Press Start Test CO o ee Test If the test fails follow these guidelines e Repeat the test twice to verify that the test Is failing consistently e Contact technical support for more troubleshooting advice 170 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 9 Performance Verification Wavelength Accuracy Standards This test measures the absorbance of a wavelength accuracy standard and compares the location of the peaks with precisely known values at up to five wavelengths By default wavelength accuracy tests are performed in absorbance mode however this test may also be performed in T mode When run
171. nd vial Cat No AC2V24 with 1 ml of sample and 9 ml of deionized water Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one Hardcheck P Tablet straight from the foil into the vial Crush the tablet with a clean stir rod Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial Wait for a reaction period of 5 minutes Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l total hardness Notes Strong alkaline or acidic water samples must be adjusted between pH 4 and pH 10 before the tablet is added use 1 mol l hydrochloric acid or 1 mol l sodium hydroxide 80 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2030 Hydrazine Powder Test Dimethylamino benzaldehyde Method 0 05 0 5 mg l N2H4 l 2 4 DW Fw 8 9 Load and run the AC2030 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in t
172. ngth Enters the beginning wavelength for a scan Scanning Thermo Scientific Orion AquaMate Spectrophotometer User Manual 205 Appendix A General Instrument Information Statistics Turns statistics on or off calculates the average and standard deviation of results when set on Statistics registers are cleared when set off when instrument Is turned off when test parameters are changed and when test is saved or resaved all test types except Kinetics Scanning Multiwavelength Std Concentration Enters the concentration of the analyte in the standard solution Stop Wavelength Enters the ending wavelength for a scan Scanning Stored Tests 7 Directory Displays the list of tests stored in the instrument Utility Tabular Displays the list of collected data Kinetics Scanning Operator enters an alphanumeric name maximum of 16 characters for the test Test Name the name will be included on the data printout and if the test is saved will be displayed in the Utility Test Directory screen available in all tests Total Run Time Enters the time from the run initiation to the end of the test equals Delay Time Interval Times Measurement Times Kinetics Selects or creates units labels for results all stored tests except Abs Ratio ane Scanning Cell Growth ns lect Test Removes the gt tag of the highlighted test name to remove the test from the SmartStart menu
173. ngth value Use the numeric keypad to enter the wavelength value for taking measurements Press the Set nm function key again to save the entered wavelength value 3 Prepare the blank and standard that will be used to calculate sample concentration 4 Place the blank in the B position and press the Measure Blank function key Once the blank has been measured remove it from the carousel 5 Press the Units Standard function key to set the units and measure the standard a Press the Enter Conc function key use the numeric keypad to enter the concentration of the standard and press the enter key b Press the Select Units function key press the A or W key to highlight the desired units and press the enter key c Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Standard function key The instrument will measure the absorbance of the standard and display the absorbance and calculated factor 3 14pm a bs Factor 510 0nm calculated Blank Conc Units Standard 6 Press the esc key to return to the main measurement mode 7 Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location The concentration measurement will be shown on the instrument display 148 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 8
174. ning the external standard test remember Wavelength accuracy standards designed to measure the wavelength accuracy at the Specified wavelengths are required Use an empty vial holder as the blank Measure the standards in the order they are listed on the test screen The wavelength Standard at only five wavelengths can be measured To run the Wavelength Accuracy Standards test FEF W N FF 6 Press the test key to access the Test Types menu Press the A or Y key to highlight Performance Verification and press the enter key Press the A or Y key to highlight Wavelength Accuracy Standards and press the enter key Enter the appropriate wavelength values and their tolerances a Press the Add nm function key use the numeric keypad to enter the wavelength value in the entry field and press the enter key b Use the numeric keypad to enter the tolerance for the entered wavelength in the entry field and press the enter key c Repeat for each standard Verify that the wavelengths and tolerances are set correctly Performance Verification 12 09pm 6Ju115 Wavelength Accuracy Standards Measurement Mode Transmittance Peak Expected Tolerance Measured Result Place Standard 1 in position 1 Press ESC to save test Mode Test Press the Start Test function key Orion AquaMate Spectrophotometer User Manual 171 Chapter 9 Performance Verification 7 The results will indicate pass or fail for each wavel
175. nk The blank is a clear Solution deionized water with zero absorbance The reagent blank is a mixture of the reagent and deionized water and provides a zero concentration point with the darkest color highest absorbance The color of samples prepared with the reagent will decrease as the concentration increases for this method Orion AquaMate Spectrophotometer User Manual 71 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate e Runsamples and blanks with the same batch of vials The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch e Do not place the hot vials in the sample chamber Cool the vials to room temperature for final measurements e Suspended solids in the vial lead to incorrect measurements For this reason itis important to place the vials carefully in the sample chamber The precipitate at the bottom of the sample should be not suspended e Clean the outside of the vials with a towel Fingerprints or other marks must be removed e Samples can be measured when the chloride content does not exceed 1000 mg l e n exceptional cases compounds contained in the water cannot be oxidized adequately so results may be lower than reference methods 72 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate CODH00 COD Mid Range Digestio
176. nsrantanmapninia 129 Selecting a Different Curve Fitfor a Standard Curve csccccssessssersssserssserssseserseseees 130 Recalling an Existing Standard Curve Method ccsccsssssssscssesssssssssesrsersteess 131 Chapter Taunan a 132 Wavelength Scanning Test Menu ssssssssssisosissnnnnononnnnnnnnnnnnnnnnnnnnannnnnnnnnnnnnnanananannnannn 132 Setting the Parameters fora SCAN sitchin deveisnseainataelevnctitasiiatieliwidaranlualisbatiinaviahiabswnailaers 133 Collecting a Baseline Scan and Scanning a SAMPLE ccccseessesessssesssssesssseeerseees 134 Viewing and Manipulating Scanned Data ccssccsssssssssssssssssessssssssssssrssserssserssserases 136 Recalling an Existing Scanning Method s s sssssrsrsrsrsrnrnunnrnrnrnrnrnnrnrnrnrnnnnrnrrrnrnrnnrerernrnns 141 CNapter Biria a aa 142 Absorbance Transmittance and Concentration Measurements ssis 142 Absorbance amp Transmittance Measurements s sssssssesrssisrnsrnrnnrnrnninrnnrnninrnnrnnrnrnnrnresrnrens 142 Using the Basic A T C Test M thod ccccssssssssssssssssssssssssssrssssssrsrersrssrseteess 142 Using the Advanced A T C Test Method ccccssecsssssssssssrsssrssrssssesssesssersteess 144 Concentaton Measurement narria 147 Using the Basic A T C Test Method s sssssrsrssrsrsrnrnrnurnrnnnrnrnrnnrnrnnnrnrnrnrnnrernrnrenrernrnrnns 147 Using the Advanced A T C Test Method s ssssssesrsrsrnrnrnsnrnrnnnnnnrnnnnrnrnnnnrnrenrnrerenreres 150 VIVO ORIN ead caster a acd tame setae eae ta
177. nstrument is compatible with most HP PCL printers and brands other than HP will also be supported if they use the PCL5 communications protocol It is your responsibility to determine whether the printer is compatible with the instrument Alternatively displayed ASCII text can be sent to a computer using a USB connection Enabling the computer as the printing device sends ASCII data to the computer via the USB connection to the computer however no graphics are sent Installing the USB Driver from the User Documentation CD 1 Connect the USB computer cable to the USB communication port on the back of the AquaMate spectrophotometer Insert the Orion AquaMate User Documentation DVD into the computer CD DVD drive Connect the USB computer cable to a USB port on the computer Open the computer Device Manager window Double click the Spectrophotometer option under Other Devices in the Device Manager Click the Update Driver button on the Spectrophotometer Properties window Click the Browse my computer for driver software button Click the Browse button O po oS ee Se OS Or Select the appropriate USB driver folder from the Orion AquaMate User Documentation DVD in the computer CD DVD drive and click the OK button a Select the Vista32 folder when using a 32 bit Microsoft 7 or Vista operating system b Select the Vista64 folder when using 64 bit Microsoft 7 or Vista operating system c Select the XP32 folder when using a Microsoft XP ope
178. nts approximately 20 seconds Wipe the exterior of the vial 8 Place the vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Sample function key to display the result in mg l free chlorine Notes Vial cleaning As many household cleaners i e dishwasher detergent contain reducing Substances the subsequent determination of chlorine may show lower results To avoid any measurement errors only use glassware free of chlorine demand Preparation Put all applicable glassware into sodium hypochlorite solution 0 1 g l for one hour and then rinse all glassware thoroughly with deionized water For individual testing of free and total chlorine the use of different sets of glassware Is recommended EN ISO 7393 2 5 3 Preparing the sample When preparing the sample the escape of chlorine gases i e by pipetting or shaking must be avoided The analysis must take place immediately after taking the sample The DPD color development is carried out at a pH value of 6 2 to 6 5 The reagents therefore contain a buffer for the pH adjustment Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the reagent is added use 0 5 mol l Sulfuric acid or 1 mol l sodium hydroxide Exceeding the measuring range Concentrations above 2 mg l chlorine using powder packs can lead to results showing 0 mg l In this event the water sample must be diluted with water free of chlorine 10 ml
179. od 1 Press the test key to access the Test Types menu 2 Press the A or W key to highlight Absorbance Difference and press the enter key 3 To recall an existing Absorbance Difference method press the Stored Tests function key 4 Press the A or Y key to highlight Load internal test or Load test from USB drive and press the enter key 5 Press the A or Y key to highlight the desired test and press the enter key 160 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements 3 Point Net The 3 Point Net application determines the height of a peak based on a sloping baseline drawn between two wavelengths on either side of the peak This type of analysis is beneficial when the precise peak height is needed for a particular assay A factor can be multiplied by the measured peak height to give the concentration of the measured analyte in the appropriate concentration units This section covers e Setting Up Test Parameters e Taking Measurements e Recalling a Test Setting the Parameters for 3 Point Net Method 1 Press the test key to access the Test Types menu 2 Press the A or W key to highlight 3 Point Net and press the enter key Test Types 2 10pm 63u115 Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scannin 5 u ltiwave length Performance Verification Start Tests
180. olor measurement by preprogrammed methods by a user generated standard curve or by multiple wavelengths if desired The following table describes some of the color methods which may be tested on the Orion AQ 7000 and AQ8000 spectrophotometers 116 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Preprogrammed Samale Color Method name or user Cell 4 Wavelength s Scope of the method defined i Platinum Cobalt Color CLRPT50 50 mm 455 nm Water and wastewater Method absorbance CLRPT25 25 mm Samples of color similar measured at one CLRPT24 24 mm to Pt Co color standards wavelength User Method 10 50 mm Range 0 500 PCU Adaptation of NCASI CLRPTP50 50 mm Color in pulp mill 253 803 absorbance CLRPTP25 25mm wastewaters measured at one CLRPTP 24 24 mm wavelength User Method 10 50 mm Range 0 500 PCU ISO 7887 GB 11903 User Method Raw and potable water Water Quality industrial water of low Examination and color Determination of Colour Tristimulus Values User Method Multiple Water and wastewater Method transmittance is wavelengths samples of color measured at multiple 400 to 700nm different from but not wavelength in the 400 range excluding Pt Co 700 nm range standards Application Notes Application notes for color testing are available through your local technical sales representative our technical support servi
181. on units When using a standard specify the concentration of the standard and measure its absorbance When using a factor specify the factor and concentration units The steps for taking measurements in the two modes are similar the only difference is if a standard is measured or a factor is entered 2 43pm aes 15 0 3090 510 Onm Blank standard nm Mods In the main measurement mode press the Change Mode function key to toggle between Absorbance Transmittance and Concentration When the mode is setto Conc Std or Conc Factor these capabilities are provided e Measuring a Blank e Measuring a Standard or Entering a Factor e Setting the Wavelength e Measuring Samples Orion AquaMate Spectrophotometer User Manual 147 Chapter 8 Absorbance Transmittance and Concentration Measurements Using the Conc Std Mode to Measure Concentration In this mode a standard sample of well Known concentration is used to determine the concentration of samples When the standard concentration is precisely Known the Abs T of the standard and the Abs T of the unknown sample are measured and the sample concentration is calculated The measurement can be expressed mathematically as Standard Concentration Sample Concentration Abs T of Standard Abs T of Unknown Sample 1 Ifnecessary press the Change Mode function key until Conc Std is set as the mode 2 fnecessary press the Set nm function key to set the wavele
182. ontents of one Phosphate Rat F10 Power Pack straight from the foil into the vial Use a funnel to add the reagent 12 Close the vial tightly with the cap and swirl the vial for 10 15 seconds to mix contents The reagent will not completely dissolve Wipe the exterior of the vial 13 Wait for a reaction period of 2 minutes 14 Place the vial into the holder in the sample chamber and close the chamber door 15 Press the Measure Sample function key to display the result in mg l acid hydrolysable phosphate as phosphorus P Notes Appropriate safety precautions and good lab technique should be used during the whole procedure Ortho phosphate ions react with the reagent to form an intense blue color Phosphate in organic and condensed inorganic forms meta pyro and polyphosphates must be converted to ortho phosphate ions before analysis Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed inorganic forms Organically combined phosphates are converted to ortho phosphate ions by heating with 106 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate acid and persulfate The amount of organically combined phosphates can be calculated mg l phosphate organic mg l phosphate total mg l phosphate acid hydrolysable e Application for water wastewater and seawater e Highly buffered samples or
183. or Y key to highlight a test and press the enter key to start the test Thermo Scientific Orion AquaMate Spectrophotometer User Manual 31 Water Analysis Test Menu Preprogrammed Methods Thermo Scientific Orion AquaMate 8000 UV Vis and AquaMate 7000 Vis spectrophotometers include a USB memory stick that contains over 260 preprogrammed methods for use with Thermo Scientific Orion AQUAfast Merck and CHE Metrics reagent chemistries Preprogrammed methods provide values for the test parameters required to run specific reagent chemistries on the instrument including wavelength vial path length concentration factors curves and measurement unit All preprogrammed methods are stored on the USB memory stick So operators can select and load only the methods needed for their applications Up to 90 methods can be stored on the AquaMate spectrophotometer and stored methods can be added or removed as needed Operators can also modify preprogrammed methods or create their own custom methods so additional parameters and test methods can be added at any time AquaMate instruments allow a one point adjustment on any preprogrammed method using a known standard to correct for variations in batch to batch reagent chemistries The following instructions are for using Orion AQUAfast reagent Merck or CHEMetrics chemistries with the AquaMate spectrophotometer Preprogrammed methods use a specific vial size path length in the formula and the vial size
184. orb light resulting in falsely high absorbance readings and 2 contaminants in the vial may react chemically with subsequent reagents or Standards introduced into the vial Cleaning methods depend to some extent on the nature of the contaminating material It is important to identify the residual material in the vial that needs to be removed Refer to the following table for suggestions on cleaning methods solvents and material Solvent Examples Suggested Cleaning Methods Warm water with detergent Aqueous Protein Biologics DNA Dilute nitric acid lt 10 rinse Copious water rinse Dilute nitric acid lt 10 rinse Aqueous Salt solutions Copious water rinse Warm water with detergent Aqueous Basic solutions Dilute nitric acid lt 10 rinse Copious water rinse Orion AquaMate Spectrophotometer User Manual 179 Chapter 10 Maintenance Solvent Examples Suggested Cleaning Methods Rinse with organic solvent Organic Hydrocarbons small molecules oils Aa ene a Dilute nitric acid lt 10 rinse Copious water rinse l l Rinse with similar alcohol acetone or other solvent Organic Alcohol solutions Copious water rinse Rinse with organic solvent Warm water with detergent eae fine so liens Dilute nitric acid lt 10 rinse Copious water rinse Rinse with organic solvent Organic Hydrocarbons small molecules oils elle cea el Dilute nitric acid lt 10 rinse Copious water rinse Important Keepin
185. orbance Transmittance and Concentration Measurements Absorbance Difference The Absorbance Difference application measures the difference in absorption at two different wavelengths Reference wavelength correction is available to eliminate the effects of a sample matrix Typically used in quality control applications an absorbance difference application provides a convenient and quick diagnostic test for sample quality This section covers e Setting Up Test Parameters e Taking Measurements e Recalling a Test Setting the Parameters for Absorbance Difference Method 1 Press the test key to access the Test Types menu 2 Press the A or V key to highlight Absorbance Difference and press the enter key Test Types Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Met Multiwavelength Performance Verification ee Start Tests ATC 3 Highlight and change the displayed test parameters including Test Name Wavelength 1 Wavelength 2 Factor 1 Factor 2 Units and Sample Positioner See the Parameters section for more information on the test parameter settings Absorbance Difference Test Name Wavelength 1 Wavelength 2 Ref tale ae Correction A Positioner of Samples More parameters Press f or to select item to change ee Se Test Tests Test 4 Press the Save Test function key and follow the onscreen prompts to save the test Orion
186. ore the reagent is added use 0 5 mol l sulfuric acid or 1 mol l sodium hydroxide Interferences When interferences occur color development is inhibited or a precipitate is formed The values below refer to a standard with an iron concentration of 0 5 mg l The following substances do not interfere when present up to the levels given Substance NointerrenceTo Substance No interference To 0 05 mal LO mal 0 6 mal ing 28 mal 84 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2055 Manganese Tablet Test Formaldoxime Method 0 2 4 mg l Mn 1 Load and run the AC2055 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Manganese LR 1 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 7 Add one Manganese LR 2 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 8 Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial 9 Wait fora reaction
187. ore the reagent is added use 0 5 mol l sulfuric acid or 1 mol l sodium hydroxide Exceeding the measuring range Concentrations above 10 mg l chlorine using tablets can lead to results showing 0 mg l In this event the water sample must be diluted with water free of chlorine 10 ml of the diluted sample should be mixed with the reagent and the measurement repeated Turbidity can lead to errors The use of the DPD No 1 tablet in samples with high calcium ion contents and or high conductivity can lead to turbidity of the sample and therefore incorrect measurements Oxidizing agents such as bromine or ozone interfere as they react in the same way as chlorine Orion AquaMate Spectrophotometer User Manual 65 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P71 Chlorine Free Powder Pack Test DPD Method 0 02 2 mg l Cl 1 Load and run the AC4P 71 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add the contents of one Chlorine Free DPD F10 Powder Pack straight from the foil into the vial 7 Close the vial tightly with the cap and swirl or invert several times to mix the conte
188. osphate Total Digestion Tube Reagent Phosphate Acid Hydrolysable Digestion Tube Reagent OO aa Orion AquaMate Spectrophotometer User Manual 47 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Orion AQUAfast Reagent Instructions The measurement ranges specified in the following test procedures are based on standard solutions measured under ideal conditions These ranges may vary due to the type of sample being measured since various interferences can have a major influence on the accuracy of the method Due to the fact that each sample is different the only way to check the tolerance precision is the Standard Additions Method According to this method first the original sample is tested Then further samples 2 to 4 are taken and small amounts of a standard solution are added and further results are obtained The amounts added range from approximately half up to double the amount present in the sample itself These supplementary results make it possible to estimate the actual concentration of the original sample by comparison Test methods and ranges are subject to change without notice For a list of the most up to date test methods visit www thermoscientific com water Recommendations for Avoiding Measurement Errors e Thoroughly clean vials caps and stir rods after each analysis in order to prevent carry over errors Even minute reagent residues lead to incorrect measurements e Ensure that t
189. ows Determine the amount of total iron present in the water sample To produce the blank add an iron Standard solution with the same iron concentration to the vial instead of deionized water Conversion mg l NH4 mg I N x 1 29 mg l NH3 mog I N x 1 22 56 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC 2035 Bromine Tablet Test DPD Method 0 05 13 mg l Br L 2 O p O e Load and run the AC203524 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Empty the vial leaving a few drops of sample remaining in the vial Add one DPD No 1 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod Add sample to the 10 ml mark on the vial Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l bromine Notes Alternatively the AC203510 method can be
190. played test parameters including Test Name Measurement Mode Start Wavelength Stop Wavelength Sample Positioner Scan Speed and Interval a See the Parameters section for more information on the test parameter settings Use the Baseline Expiration Setting to seta baseline expiration time ll If Auto Save Data is set On enter a Data File Name before accessing the Run Test or Measure Samples functions b See the Sample Positioner Setting for the Vial Holder Carousel section for detailed information on selecting the appropriate Sample Positioner setting Scan tests only measure one sample ata time so Auto 6 and Auto 3 Sample Positioner settings are not fully functional 3 58pm a a SCAN_O1 Auto Save Data Press f or to select item to change ee Se Test Tests Test 4 Press the Save Test function key and follow the onscreen prompts to save the test Orion AquaMate Spectrophotometer User Manual 133 Chapter 7 Wavelength Scanning Test Menu Collecting a Baseline Scan and Scanning a Sample Note If a 3 position or 6 position vial holder carousel is in use the blank must be placed in the B position The instrument always uses the B position to collect the baseline Once the parameters have been set for the scan 1 Press the Run Test function key 2 Prepare the blank and sample that will be scanned 3 Place the blank in the B position on the vial holder carousel and press the Collect Baseline function
191. ples are in the appropriate positions on the carousel and press the enter key c Once the blank and samples have been measured remove them from the carousel d To measure additional samples press the Measure Sample function key and repeat Steps 3a through 3c 4 If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting a Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank has been measured remove it from the carousel b Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Sample function key Repeat for each sample 5 When all of the samples have been measured press the Save Data function key and follow the onscreen prompts to save the sample measurements to the USB memory stick Recalling an Existing Absorbance Ratio Method 1 Press the test key to access the Test Types menu 2 Press the A or W key to highlight Absorbance Ratio and press the enter key 3 To recall an existing Absorbance Ratio method press the Stored Tests function key 4 Press the A or Y key to highlight Load internal test or Load test from USB drive and press the enter key 5 Press the A or Y key to highlight the desired test and press the enter key 158 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 8 Abs
192. ppears e fyou select one test as a SmartStart test when the instrument is powered on it will automatically load this test for immediate measurement e lf you select more than one test as SmartStart tests when the instrument is powered on it will automatically display a menu containing only those tests Note The default menu can always be accessed by pressing the test key 30 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 3 Orion AquaMate Instrument Setup Setting a SmartStart Test 1 Press the utility key to access the Utility menu Utility 9 59am 26Mari5 Software Revision 1 6004 Instrument Serial Number W2R091104 Printer PC Date Time Setup 9 59am 26Mari5 Standby i Baseline Expiration Chrimind Keeper Language of lamp life used 2 Press the A or W key to highlight Stored Tests Directory and press the enter key 3 Press the A or Y key to highlight Load internal test and press the enter key 4 Press the A or Y key to highlight the appropriate test and press the Select Test function key An arrow icon gt next to the test name indicates the test has been selected for the SmartStart menu Utility Test Directory 10 07am 46Mari5 6MariS 6Mari5 6Mari5 G areae Te Test Test Test Press the esc key to return to the Utility menu Press the test key to access the Test Types menu Press the Smart Start function key to open the Smart Start menu o Se Se p Press the A
193. quation 1 Load the test method in the Water Analysis test menu 2 Ifthe 1pt Adjust option is set to Off press the A or W key to highlight 1pt Adjust and press the enter key to set the parameter to On 9 54am 24 MariS BLANK AC4P71 Absorbance 510 0nm On 1 600 orf Manual A Auto Save Data More parameters Press f or to select item to change ipt Adjust Test Tests Test 3 Prepare a blank reagent blank reverse color methods only and calibration standard with a known concentration within the method range and near the expected sample concentration Refer to the specific reagent chemistry instructions for detailed information 4 Press the Calculate 1pt Adjust function key 5 Enter the concentration of the standard using the numeric keypad and press the enter key Water Analysis 9 57am 2 7MariS Test Mame AC4P71 BLANK Standard Concentration Entry Enter a number 0 000 to 9999 6 Wipe the exterior of the blank vial and place the blank vial into the holder in the sample chamber and close the chamber door 40 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific 8 9 10 11 12 13 14 15 16 Chapter 4 Water Analysis Test Menu Press the Measure Blank function key Open the sample chamber door and remove the vial from the holder For reverse color methods only Wipe the exterior of the reagent blank vial and place the reagent blank via
194. r press the enter key use the numeric keypad to enter the factor value and press the enter key Repeat for each required equation factor Save or run the test using the new test parameters Orion AquaMate Spectrophotometer User Manual 45 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Orion AQUAfast Colorimetric Reagents Compatible with Orion AquaMate Instruments Use the information in the following table to identify the Orion AQUAfast reagent method file name on the USB memory stick and the test parameters associated with each method This information is also included in the Orion AquaMate Master Methods List available on the Orion AquaMate user documentation CD or on our website at www thermoscientific com water Parameter Part Method Description rore Free amp Total Tablet Reagent Free Powder Pack Reagent 46 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Parameter Part Method Description Chlorine Total Powder Pack Reagent Chlorine Total High Range Tablet Reagent Copper Free amp Total Tablet Reagent Copper Free Powder Pack Reagent Hardness Total Low Range Tablet Reagent Hardness Total High Range Tablet Reagent Nitrogen Total Low Range Digestion Tube Reagent Nitrogen Total High Range Digestion Tube Reagent Phosphate Ortho Low Range Tablet Reagent Ph
195. r 2 Spectrophotometer Basics Instrument Connections The following connectors are on the back of the instrument USB Printer Port Fuse Compartment USB Computer Port AC Power Connector On Off Switch Warning Avoid shock hazard Always turn off the instrument and unplug it from the wall outlet Or power strip before you unplug the power cord from the instrument connector Instrument Keypad Orion AquaMate Spectrophotometer User Manual 13 Chapter 2 Spectrophotometer Basics a Function keys Press to perform the action shown above each key on the amp A amp instrument display The function keys will change depending on the displayed screen Some function keys may not be active on the display Escape key Press to clear a value being entered or return to a previous screen O Clear key Press to clear the last character entered Enter key Press to accept highlighted entered or selected values or press to enter advance to the next parameter or screen Numeric keys Use to enter numbers a decimal point or minus sign to make a value negative Arrow keys Press to move the location of the curser or press to highlight a value or option from a list of selections O Utility key Press to access the utility menu and instrument setup options O Test key Press to access the test menu with list of available analysis modes Print key Press to export results to a printer or computer when PC is sel
196. r detergent contain reducing Substances the subsequent determination of chlorine may show lower results To avoid any measurement errors only use glassware free of chlorine demand Preparation Put all applicable glassware into sodium hypochlorite solution 0 1 g l for one hour and then rinse all glassware thoroughly with deionized water For individual testing of free and total chlorine the use of different sets of glassware Is recommended EN ISO 7393 2 5 3 Preparing the sample When preparing the sample the escape of chlorine gases i e by pipetting or shaking must be avoided The analysis must take place immediately after taking the sample The DPD color development is carried out at a pH value of 6 2 to 6 5 The reagents therefore contain a buffer for the pH adjustment Strong alkaline or acidic water samples 62 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate must be adjusted between pH 6 and pH 7 before the reagent is added use 0 5 mol l Sulfuric acid or 1 mol l sodium hydroxide e Exceeding the measuring range Concentrations above 10 mg l chlorine using tablets can lead to results showing 0 mg l In this event the water sample must be diluted with water free of chlorine 10 ml of the diluted sample should be mixed with the reagent and the measurement repeated e Turbidity can lead to errors The use of the DPD No 1 tablet in
197. r methods in the Merck and CHE Metrics folders is available in the Thermo Scientific Orion AquaMate Master Methods List and on the manufacturer s website Note A copy of the methods on the USB memory stick should be saved to a computer or other data source in case the USB memory stick is accidentally deleted or otherwise corrupted Thermo Scientific Orion AquaMate Spectrophotometer User Manual 33 Chapter 4 Water Analysis Test Menu Loading Test Methods from USB Stick to AquaMate Instrument l 2 Open the AquaMate USB memory stick using a computer Copy the desired test methods from the Orion Merck and CHEMetrics folders to the Thermo folder Remove the USB memory stick from the computer and insert it into the USB port on the front of the AquaMate instrument When the AquaMate instrument is first powered on it will display the initial measurement screen Press the test key to access the Test Types menu Absorbance 10 59am 24240ct12 0 9018 450nnm Blank m Moda Press the A or Y key to highlight Water Analysis and press the enter key Test Types 11 00am 440cti2 Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Net Multiwavelength Performance Verification Press fT or to select start Teete ATC Press the Stored Tests function key Water Analysis 11 04am 440cti2 BLANK Test Name AC4SP71 Saved Measurement Mode Wave length ipt Adjust
198. r rod 11 Close the vial tightly with the cap and swirl or invert several times until the tablets are dissolved Wipe the exterior of the vial 12 Wait for a reaction period of 1 minute 13 Place the vial into the holder in the sample chamber and close the chamber door 14 Press the Measure Sample function key to display the result in mg l silica Notes Thermo Scientific The tablets must be added in the correct sequence Phosphate ions do not interfere under the given reaction conditions If phosphate is known to be absent the addition of the Silica PR Tablet may be omitted Orion AquaMate Spectrophotometer User Manual 109 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P60 Silica Powder Pack Test Silicomolybdate Method 1 90 mg l Si02 1 Load and run the AC4P 60 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Temperature of the sample should be 15 C to 25 C Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add the contents of one Silica HR Molybdate F10 Powder Pack straight from the foil into the vial 7 Close the vial tightly with the cap and swirl or invert several times to mix the contents 8 Add the
199. ra TS 154 PDS ONO ING ER GUO eset ate Sa ccd heaters E een ona si omer eaten 157 ADS OND ANC Ce DINCTENCE rit se snatttnticd einai ghia desinedunated a abentncnd nen thet 159 BoP OMA NS Earra eteseaattastina tates get usage O te leeiaa auttatiee sana ameter 161 FU CGS AE E AEEA E E AEE AT AE EAE AT ET AE ETE EET 163 Chapter Sanos pias isinedeliaeamarenibeenmantinis 168 Performance Vernicad frmnannnonnnna 168 Performance Verification MESS iniiaiee ai ae 168 Accessing the Performance Verification Tests s ssssssesssrsresrsrnresnnrnrnnrnrnnnnrnrennrnrrnrernrrnrrrerens 169 OCS MOO UGC NECKS Darra vila teen acini seiamananaaanienal anneal 169 Wavelenoth ACCURACY IMEI cites sacs eis atsoutiate inch att sabes A 170 Wavelength Accuracy lt 5 Ta NCA OS sarirsi cexiuiscnawtiarropianan esmustatcndaisgnctatssiatedcantedeenneansancutnernitants 171 PFU COUT SUING C CUI CY aioin aia aeo AE A N 173 NGISCMSAS UTI ACE sca ted cecnsactsiatactva tue daassatistnchsactsatitae Cac aieaty tdi Canvlonatrars aac otanae tides Caneaat 175 STAV LON rnanan A Ea 176 Chapter LO izicsiesavstvapassauavsvidatvagedsancantaiaavasisuluavsutucbarassavanntuaievanivsnniunienavesiingnunnineilt 178 Mantenan eannan TARA 178 Routine C ale asorar n a O 179 Cleaning and Maintaining Vials and CuvetteS s ssssssssrsrsrsrnrnrrnrsrrnrnrnrnrrnrnrnrnrrnrnrnrererenns 179 Cleaning the Windows of the Sample COmMpatment s ssssssrssesrsrrrrrnrnrnrrsrererernrrrrerererenns 181 CDan EE acacia
200. ral times until the tablet is dissolved Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Sample function key to display the result in mg l free chlorine Open the sample chamber door and remove the vial from the holder Add one DPD No 3 Tablet straight from the foil into the vial Crush the tablet with a clean Stir rod Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial Wait for a reaction period of 2 minutes Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Sample function key to display the result in mg l total chlorine Notes Alternatively the AC207010 method can be used with 10mm square vials and the AC207050 method can be used with 50mm rectangular vials All blank and sample volumes must remain the same as those specified in these instructions so Samples may need to be prepared in separate containers and then transferred into the selected vial Vial cleaning As many household cleaners i e dishwasher detergent contain reducing Substances the subsequent determination of chlorine may show lower results To avoid any measurement errors only use glassware free of chlorine demand 60 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry
201. rating system 10 Click the Next button 11 Wait while the USB driver software is located and installed a Approve any security messages by clicking the Install button 12 Once the installation of the USB driver software is complete click the Close button 13 Record the COM port number of the USB connection in the Device Manager window Orion AquaMate Spectrophotometer User Manual 23 Chapter 3 Orion AquaMate Instrument Setup Date Time Setup Setting 1 Inthe Utility menu press the A or W key to highlight the Date Time Setup option and press the enter key to open the Date Time Setup menu 1 29pm 25Mari5 2 5MariS 2 Tosetthe date press the A or Y key to highlight the Set Date option qd Press the Set Day function key use the numeric keypad to enter the day value and press the enter key Press the Set Month function key press the A or Y key to highlight the correct month and press the enter Key Press the Set Year function key use the numeric keypad to enter the year value and press the enter key 3 Tosetthe time format press the A or Y key to highlight the Time Format option and press the enter key to toggle the format between am pm and 24 hours 4 Tosetthe time press the A or Y key to highlight the Set Time option qd 24 Orion AquaMate Spectrophotometer User Manual Press the Set Hour function key use the numeric keypad to enter the hour value and press the enter key Press the Set Minut
202. ress the utility key to acess the instrument utility menu Press the A or Y key to highlight the Printer option and press the enter key to open the printer menu Utility 11 55am 43Jun14 Software Revision 1 004 Instrument Serial Number W24K091104 Printer oF 11 55am 243Juni4 min On Eng lish 0 557 Calculator Press f or to select i Orion AquaMate Spectrophotometer User Manual 195 Chapter 11 Customer Services 4 Press the A or V key to highlight the External option and press the enter key to set the External printer option to On Utility 3 19pm 24Jun14 Software Revision External Press f or to select Press ENTER to change Press ESC to exit Utility Software Revision Instrument Serial Number Pr inter Date Time Setu 3 22pm 24 Juni4 Standby i Baseline Expiration Chrimind Keeper Language OF lamp life used Screen Contrast Printout Contrast Stored Tests Directory USB Drive Files Calculator Press f or to select B USB Printer Cable Interfacing and Printing Data 1 Connect the USB cable to the USB printer port on the back of the AquaMate Spectrophotometer USB printer port 2 Connect the USB printer cable to the USB port on the printer 3 When data from the AquaMate spectrophotometer is ready to be sent to the printer press the print key on the AquaMate spectrophotometer 196 Orion AquaMate Spectrophotometer User Manual Thermo S
203. ries Water and Laboratory Products North America Germany India Toll Free 1 800 225 1480 Tel 49 6184 90 6000 Tel 91 22 4157 8800 Tel 1 978 232 6000 info water uk thermofisher com Wai asia thermofisher com Info water thermofisher com China Singapore Tel 86 21 68654588 Tel 65 6778 6876 wal asia thermofisher com wal asia thermofisher com 269 289800 0715 RevC Japan Tel 81 045 453 9175 wai asia thermofisher com Australia Tel 613 9757 4300 In Australia 1300 735 295 InfoW aterAU thermofisher com Thermo SCIENTIFIC A Thermo Fisher Scientific Brand
204. rion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2071 Chlorine Free Tablet Test DPD Method 0 01 6 mg l Cl 1 Load and run the AC207124 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Empty the vial leaving a few drops of sample remaining in the vial 7 Add one DPD No 1 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 8 Add sample to the 10 ml mark on the vial 9 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l free chlorine Notes Alternatively the AC207110 method can be used with 10mm square vials and the AC207150 method can be used with 50mm rectangular vials All blank and sample volumes must remain the same as those specified in these instructions so Samples may need to be prepared in separate containers and then transferred into the selected vial Vial cleaning As many household cleaners i e dishwashe
205. rra Press the Smoothing function key to smooth the data if it shows sampling noise b Press the 3 Pt Net function key to determine the peak height using a 3 point net equation The 3 point net measurement screen shows the cursor options and three Cursor lines designated for the left center and right wavelengths Press the Cursor and Cursor function keys to position the left cursor line at the desired wavelength value press the Next Cursor function key and repeat for the center cursor line and right cursor line Press the Enter Factor function key to access the set factor box use the numeric keypad to enter the desired factor value and press the enter key The instrument will calculate the value for the 3 point net absorbance for the selected wavelengths multiplied by the selected factor Scanning 2 42pm 16AprisS Test Name SCAN_O1 475 500 525 0 191 0 305 0 354 0 370 Wave length 550 0 0 033 Q 00 a 1 r ex Factor Cursor Cursor c Press the Area function key to calculate the area under the curve 138 Orion AquaMate Spectrophotometer User Manual Press the Cursor and Cursor function keys to position the left cursor line at the desired wavelength value Press the Next Cursor function key Press the Cursor and Cursor function keys to position the right cursor line at the desired wavelength value Thermo Scientific Thermo Scientific d Chapter 7 Wavelength Scanning Test Menu
206. s To avoid any measurement errors only use glassware free of chlorine demand Preparation Put all applicable glassware into sodium hypochlorite solution 0 1 g l for one hour and then rinse all glassware thoroughly with deionized water For individual testing of free and total chlorine the use of different sets of glassware is recommended EN ISO 7393 2 5 3 Preparing the sample When preparing the sample the escape of chlorine gases i e by pipetting or shaking must be avoided The analysis must take place immediately after taking the sample The DPD color development is carried out at a pH value of 6 2 to 6 5 The reagents therefore contain a buffer for the pH adjustment Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the reagent is added use 0 5 mol l Sulfuric acid or 1 mol l sodium hydroxide Exceeding the measuring range Concentrations above 2 mg l chlorine using powder packs can lead to results showing 0 mg l In this event the water sample must be diluted with water free of chlorine 10 ml of the diluted sample should be mixed with the reagent and the measurement repeated Oxidizing agents such as bromine or ozone interfere as they react in the Same way as chlorine Orion AquaMate Spectrophotometer User Manual 67 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC3072 Chlorine Total High Range Tablet Test KI Acid Method ha L 2 Pr PY
207. s been measured remove it from the carousel b Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Sample function key Repeat for each sample 5 When all of the samples have been measured press the Save Data function key and follow the onscreen prompts to save the sample measurements to the USB memory Stick Recalling an Existing 3 Point Net Method 1 Press the test key to access the Test Types menu 2 Press the Aor Y key to highlight 3 Point Net and press the enter key 3 To recall an existing 3 Point Net method press the Stored Tests function key 4 Press the A or Y key to highlight Load internal test or Load test from USB drive and press the enter key 5 Press the A or Y key to highlight the desired test and press the enter key 162 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements Kinetics The Kinetics application measures the change in the sample absorbance as a function of time The local control software allows the determination of a linear rate over a particular region which can be defined after the data acquisition Frequently used in enzymatic Kinetics a factor can be multiplied by the slope of the linear rate fit to determine activity This section covers e Setting Up Test Parameters e Taking Measurements e Recallin
208. s from the curve e To measure samples using the standard curve and to view and save measurement data e To recall existing standard curve methods 122 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 6 Standard Curve Test Menu Setting the Parameters for a Standard Curve Press the test key to access the Test Types menu Press the A or Y key to highlight Standard Curve and press the enter key Test Types 11 07am 4 7MariS Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Met Multiwavelength Performance Verification Press T or to select start Teste ATC Highlight and change the displayed test parameters including Test Name Wavelength Curve Fit Number of Standards Units and Sample Positioner Date Standards Measured Wave length Ref Wavelength Correction Curve Fit Humber of Standards Units Sample Positioner Humber of Samples More parameters Press f or to select item to change statiantel Sst Sees Standards Test Tests a See the Parameters section for more information on the test parameter settings b See the Sample Positioner Setting for the Vial Holder Carousel section for detailed information on selecting the appropriate Sample Positioner setting Standard Curve 10 18am eo Test Name CHLOR INE_CURVE Date Standards Measured Wave length Ref Wavelength Correction
209. samples with extreme pH values should be adjusted between pH 2 and pH 10 before analysis with 1 mol l hydrochloric acid or 1 mol l sodium hydroxide e Phosphate ortho Phosphorus reactive e Interferences Large amounts of turbidity may cause inconsistent results mw we ron Nickel Silica Silicium dioxide e Conversions mg l PO mg l P x 3 07 mg l P205 mg IP x 2 29 Thermo Scientific Orion AquaMate Spectrophotometer User Manual 107 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2060 Silica Tablet Test Silicomolybdate Method 0 05 4 mg l SiO2 1 Load and run the AC2060 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Silica No 1 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 7 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved 8 Wait for a reaction period of 5 minutes 9 Add one Silica No 2 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 10 Close the vial tightly with the cap and swirl or invert several times until the tab
210. seconds to mix contents Wait for a reaction period of 3 minutes Open the digestion vials and add the contents of one TN Reagent B Power Pack straight from the foil into each vial Use a funnel to add the reagent Close the vials tightly with the caps and shake the vials for at least 15 seconds to mix contents The reagent will not completely dissolve Wait for a reaction period of 2 minutes Open two TN Acid LR HR Reagent C Vials and add 2 ml of the digested treated blank to the first vial this is the blank vial Add 2 ml of the digested treated sample to the second vial this is the sample vial Close the vials tightly with the caps and gently invert the vials at least 10 times to mix contents Hold the vial in a vertical position with the cap pointing up Turn the vial upside down Wait for all of the solution to flow down to the cap Return the vial to the upright position Wait for all the solution to flow to the bottom of the vial This process is one inversion 10 inversions equal about 30 seconds Wipe the exteriors of the vials CAUTION The vials will become warm during mixing Wait for a reaction period of 5 minutes Load and run the ACD007 method Place the blank vial into the holder in the sample chamber and close the chamber door 94 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate 19 Press t
211. sis with 1 mol l hydrochloric acid or 1 mol l sodium hydroxide e Phosphate ortho Phosphorus reactive e Interferences Large amounts of turbidity may cause inconsistent results mw we ron Nickel Silica Silicium dioxide e Conversions mg l PO mg l P x 3 07 mg l P205 mg IP x 2 29 Thermo Scientific Orion AquaMate Spectrophotometer User Manual 105 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ACDO95AH Phosphate Acid Hydrolysable Digestion Tube Test Acid Digestion Ascorbic Acid Method 0 02 1 6 mg l P Phosphate as Phosphorous 1 Open one 16 mm PO4 P Acid Reagent Digestion Tube and add 5 ml of sample 2 Close the vial tightly with the cap and gently invert the vial several times to mix the contents 3 Heat the vial for 30 minutes in the preheated reactor at a temperature of 100 C 4 CAUTION The vial will be hot Remove the vial from the reactor and allow It to cool to room temperature 5 Open the cooled digestion vial and add 2 ml of 1 00 N Sodium Hydroxide Solution to the vial 6 Close the vial tightly with the cap and gently invert the vial several times to mix the contents Wipe the exterior of the vial 7 Load and run the ACD095AH method 8 Place the vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Blank function key to measure the blank 10 Open the sample chamber door Remove the vial from the holder 11 Add the c
212. st Phosphomolybdenum Ascorbic Acid 0 06 2 5 mg l PO L 2 a eS 8 9 Load and run the AC4P 95 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add the contents of one Phosphate Rgt F10 Powder Pack straight from the foil into the vial Close the vial tightly with the cap and swirl or invert several times for 10 15 seconds to mix contents The powder will not dissolve completely Wipe the exterior of the vial Wait for a reaction period of 2 minutes Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l ortho phosphate Notes Ortho phosphate ions react with the reagent to form an intense blue color Phosphate in organic and condensed inorganic forms meta pyro and polyphosphates must be converted to ortho phosphate ions before analysis Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed Inorganic forms Organically combined phosphates are converted to ortho phosphate ions by heating with acid and persulfate The amount of organically combined phosphates can be c
213. sting scan method press the Stored Tests function key 4 Press the A or Y key to highlight Load internal test or Load test from USB drive and press the enter key 5 Press the A or Y key to highlight the desired test and press the enter key Utility Test Directory 3 57pm 16Apris Scanning 2SCAN_O1 15Apris Press f or to select test errr dbase Tater tear Test Test Test Orion AquaMate Spectrophotometer User Manual 141 Absorbance Transmittance and Concentration Measurements Absorbance amp Transmittance Measurements Using the Basic A T C Test Method Basic A T C mode puts the instrument into an instant measurement mode The user simply walks up to the instrument inserts a sample and measures it Depending on whether the mode is set to Absorbance A Transmittance T or Concentration the measurement results appear along with the type of measurement date and time wavelength and vial position In the main measurement mode press the Change Mode function key to toggle between Absorbance Transmittance and Concentration Absorbance 4 01pm a O O96A 560 Onm iL See Blank Mode 142 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements When Basic A T C is set to Absorbance or Transmittance these capabilities are provided e Setting the Wavelength e Measuring a Blank e M
214. sult Abs WL2 x Factor Result Abs Ratio Abs Diff Multiwavelength Orion AquaMate Spectrophotometer User Manual 203 Appendix A General Instrument Information Enters a factor to convert a datum to a result pala Abs WL3 31 x Factor Result Multiwavelength Graph Displays the graph of collected data Kinetics Scanning D Enters the numeric identifier for measurement auto increments during the test until turned off Set to 0 ae ena Displays the serial number of the instrument Utility Intercept Enters where the line crosses the Y axis Abs where concentration 0 Interval Enters the wavelength range between data points Scanning Interval Time Enters the time between repeated readings Kinetics Linearity Value Enters a linearity value Kinetics To help determine the linearity of the reaction during the measurement the instrument offers a linearity parameter This is the difference between the changes in absorbance of two measurements as shown in the following example Time Abs A Linearity 1 l 2 2 1 3 29 09 P 4 38 09 P 5 46 08 P 6 52 06 F Linearity is the A between A calculations P Pass and F Fail Load Test Loads the highlighted test from the Stored Tests Directory into active memory and sets the instrument to the test parameters Utility Lock Unlock Used to protect stored tests from accidental deletion or alteration asks for a passwor
215. t all levels 110 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P82 Sulfate Powder Pack Test Barium Sulfate Turbidity Method 5 100 mg l S04 L 2 x DoW FF w 8 9 Load and run the AC 4P 82 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add the contents of one Sulpha 4 F 10 Powder Pack straight from the foil into the vial Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial Wait for a reaction period of 5 minutes Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l sulfate Notes If sulfate ions are present a cloudy solution will appear Orion AquaMate Spectrophotometer User Manual 111 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2016 Sulfide Tablet Test DPD Catalyst Method 0 04 0 5 mg l S 1 Load and run the AC2016 method 2 Filla clean AQUAfast 24mm round vial Cat
216. t are subject to change without notice All technical information in this document Is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any terms and conditions of sale which terms and conditions of sale shall govern all conflicting information between the two documents For Research Use Only This instrument is not a medical device and is not intended to be used for the prevention diagnosis treatment or cure of disease Warning Avoid an explosion or fire hazard This instrument is not designed for use in an explosive atmosphere Thermo Scientific Orion AquaMate Spectrophotometer User Manual 11 cHAPTER 2 Spectrophotometer Basics Spectrophotometer Components The following are some of the major components visible on the outside of the instrument Sample Compartment USB Port 12 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific Chapte
217. t being run included with the description of each test Make sure to follow the instructions for the test properly Make sure filters and standards are clean Check that the sample compartment door is closed during the test Check that the sample compartment is clear of obstructions Make sure the vial holder carousel is installed properly If the 6 position vial holder carousel is installed run the test once with the sample compartment door open to verify that the carousel is moving smoothly Check that no problems are indicated by the power on diagnostics after you turn the instrument power OFF and then ON Make sure the lamp is ON Make sure the lamp compartment Is clear of obstructions Warning Do not open the lamp compartment unless the instrument power is OFF Warning Do not turn the instrument power ON unless the lamp compartment is closed Orion AquaMate Spectrophotometer User Manual 169 Chapter 9 Performance Verification Wavelength Accuracy Internal This test locates the peaks of the internal xenon lamp UV Vis instruments or internal didymium filter Vis instruments and displays the expected and measured wavelengths for the peaks e UV Vis A xenon lamp has strong fundamental lines at 229 nm 529 nm and 883 nm These lines are an essential property of xenon and serve as a fundamental standard e Vis Didymium glass has strong transmission peaks at 585nm and 80 nm These peaks are well characterized a
218. t the desired test and press the enter key Orion AquaMate Spectrophotometer User Manual 165 Chapter 8 Absorbance Transmittance and Concentration Measurements Recalling and Recalculating Graphical Kinetics Results The Kinetics application allows the viewing and manipulation of results in graphical or tabular form When the results are displayed the range start and stop time can be modified and the instrument recalculates the reaction rate When working with graphical kinetics results press the Edit Graph function key before you can rescale and recalculate You can modify the scale of your kinetics data plot automatically or manually When you select Auto Scale the instrument automatically scales the X and Y axes so all the data appears on the plot When you select Manual Scale you select specific minimum and maximum values for the X and Y axes Whenever you modify the scale the instrument recalculates and displays the new reaction rate and result The edit screen allows you to use Auto Scale to change the scale display the new graph and recalculate the results use Manual Scale to change the scale display the new graph and recalculate the results and use the cursor to select new minimum or maximum values for the X axis and recalculate the results To use Auto Scale 1 With Kinetics data displayed on the Edit Graph screen press the Auto Scale function key 2 The instrument adjusts the minimum and maximum values for the X
219. t will be measured 3 If Auto 6 or Auto 3 is selected for the Sample Positioner setting a Place the blank and samples in the appropriate positions on the carousel b Confirm that the blank and samples are in the appropriate positions on the carousel and press the enter key c Once the blank and samples have been measured remove them from the carousel d To measure additional samples press the Measure Sample function key and repeat Steps 3a through 3c 4 If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting a Place the blank in the B position on the vial holder carousel and press the Measure Blank function key Once the blank has been measured remove it from the carousel b Place the sample in the appropriate position on the vial holder carousel If needed use the Sample Positioner keypad to set the vial location Press the Measure Sample function key Repeat for each sample Advanced A xT C 2 2epm 21AprisS Test Name BLANK Page 1 Samples 1 3 sie ps TEs Blank Data Samp le 5 When all of the samples have been measured press the Save Data function key and follow the onscreen prompts to save the sample measurements to the USB memory stick Thermo Scientific Orion AquaMate Spectrophotometer User Manual 145 Chapter 8 Absorbance Transmittance and Concentration Measurements Recalling an Existing Advanced A T C Method 1 Press the test key to access the Test Types menu
220. tavanadate and bismuth Copper II ions may cause lower test results as they accelerate the decomposition of the diazonium salt It is unlikely in practice that these interfering ions will occur in such high concentrations that they cause significant reading errors Conversion mg l NO2 mg IN x 3 29 90 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC4P46 Nitrite Powder Pack Test Diazotization Azo Method 0 01 0 3 mg I N Nitrite as Nitrogen L 2 a ek es ae ae 8 9 Load and run the AC4P 46 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add the contents of one Nitri 3 Powder Pack straight from the foil into the vial Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial Wait for a reaction period of 20 minutes Place the vial into the holder in the sample chamber and close the chamber door 10 Press the Measure Sample function key to display the result in mg l nitrite as N Notes Thermo Scientific Interferences o Strong oxidizing and
221. ted The Orion AquaMate user documentation CD includes the following e Orion AquaMate Vis and UV Vis Spectrophotometer User Guide e Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate Spectrophotometers e Orion AquaMate Master Method List e Orion AquaMate Site and Safety Information e Orion AquaMate Getting Started Guide e Orion AquaMate Declaration of Conformity e Orion Laboratory Product Warranty Card e USB Driver Intended Use Please read this user manual thoroughly Any use outside of these instructions may invalidate the instrument warranty and cause permanent damage to the instrument 10 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 1 Spectrophotometer Introduction safety and Special Notices Make sure you follow the precautionary statements presented in this user manual The safety and other special notices appear in boxes Safety and special notices include the following Note Contains helpful supplementary information Important Instructions that must be followed to avoid damaging system hardware or data loss Caution Statements that indicate a hazardous situation that if not avoided could result in minor or moderate injury Warning A hazardous situation that if not avoided could result in death or serious injury Thermo Fisher Scientific provides this document to its customers with a product purchase to use in the product operation The contents of this documen
222. ter Search for driver software in this location i Include subfolders gt Let me pick from a list of device drivers on my computer This list will show installed driver software compatible with the device and all driver software in the same category as the device Thermo Scientific Orion AquaMate Spectrophotometer User Manual 189 Chapter 11 Customer Services Select the appropriate USB driver folder from the Orion AquaMate Documentation DVD in the computer CD DVD drive and click the OK button a Select the Vista32 folder when using a computer with 32 bit Microsoft 7 or Vista operating system b Select the Vista64 folder when using a computer with 64 bit Microsoft 7 or Vista operating system C Select the XP 32 folder when using a computer with Microsoft XP operating system Select the folder that contains drivers for your hardware 4 amp Computer gt g OSDisk C 4 DVD RW Drive D Thermo gt J Thermo Scientific Vista32 J Vista64 XP32 Folder Vista32 10 Click the Next button Oo Update Driver Software Spectrophotometer Browse for driver software on your computer Search for driver software in this location Include subfolders gt Let me pick from a list of device drivers on my computer This list will show installed driver software compatible with the device and all driver software in the same category as the device 190 Orion AquaMate Spectrophotomet
223. terfering substances that resulted in a concentration change of 10 Bromide more than 60 mg l and chloride more than 1000 mg l produce positive interferences Orion AquaMate Spectrophotometer User Manual 93 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ACD007 Nitrogen Total High Range Digestion Tube Test Persulfate Digestion Method 5 150 mg l N Nitrite as Nitrogen l 10 11 12 l3 14 15 16 17 18 Open two TN Hydroxide HR Digestion Vials and add the contents of one TN Persulfate Reagent Power Pack straight from the foil into each vial Use a funnel to add the reagent Wipe off any persulfate reagent that may get on the lid or tube threads Add 0 5 ml of deionized water to the first digestion vial this is the blank vial Add 0 5 ml of sample to the second digestion vial this is the sample vial Close the vials tightly with the caps and shake the vials for at least 30 seconds to mix contents The reagent many not dissolve completely Heat the digestion vials for 30 minutes in the preheated reactor at a temperature of 100 C CAUTION The vials will be hot Remove the digestion vials from the reactor and allow them to cool to room temperature Open the cooled digestion vials and add the contents of one TN Reagent A Power Pack Straight from the foil into each vial Use a funnel to add the reagent Close the vials tightly with the caps and shake the vials for at least 15
224. the vials for 120 minutes in the preheated reactor at a temperature of 150 C 5 CAUTION The vials will be hot Remove the vials from the reactor and allow them to cool to 60 C or less Gently invert the vials several times to mix the contents while still warm Allow the vials to cool to room temperature before measuring Wipe the exteriors of the vials 6 Load and run the CODLOO method 7 Filla clean AQUAfast 16mm round vial Cat No AC2V16 with deionized water this is the blank vial Close the vial tightly with the cap Wipe the exterior of the vial 8 Place the blank vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Blank function key to measure the blank 10 Open the sample chamber door Remove the blank vial from the holder 11 Place the reagent blank vial into the holder in the sample chamber and close the chamber door 12 Press the Measure Rgnt Blank function key to measure the reagent blank 13 Open the sample chamber door Remove the reagent blank vial from the holder 14 Place the sample vial into the holder in the sample chamber and close the chamber door 15 Press the Measure Sample function key to display the result in mg l oxygen Notes Reverse color methods use a reagent that when prepared with samples deceases in color as the concentration of the species being measured in the samples increases Reverse color methods require the use of both a blank and a reagent bla
225. thod 0 5 66 mg l MoO Equivalent to 0 3 40 mg l Mo L 2 Pr PE a 9 Load and run the AC 4P 42 method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add the contents of one Molybdenum HR 1 F10 Powder Pack straight from the foil into the vial Close the vial tightly with the cap and swirl or invert several times to mix the contents Add the contents of one Molybdenum HR 2 F10 Powder Pack straight from the foil into the Same vial Close the vial tightly with the cap and swirl or invert several times to mix the contents Add the contents of one Molybdenum HR 3 F10 Powder Pack straight from the foil into the Same vial Close the vial tightly with the cap and swirl or invert several times to mix the contents Wipe the exterior of the vial Wait for a reaction period of 5 minutes 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l molybdate Notes F ilter turbid water samples using filter paper and funnel before analysis Highly buffered water samples or extreme pH values should be adjusted to a pH of nearly 7 with 1
226. to save the sample measurements to the USB memory stick Thermo Scientific Orion AquaMate Spectrophotometer User Manual 155 Chapter 8 Absorbance Transmittance and Concentratio Reca n Measurements lling an Existing Multiwavelength Method 1 Press the test key to access the Test Types menu 2 Press the Aor Y key to highlight Multiwavelength and press the enter key Test Types P Water Analysis Advanced A xT C Standard Curve Absorbance Ratio Absorbance Difference Kinetics Scanning 3 Point Met ultiwvave length Performance Verification ress fT or to select Start Tests ATC 3 To recall an existing multiwavelength method press the Stored Tests function key 4 P ress the A or Y key to highlight Load internal test or Load test from USB drive and press the enter key 5 P 156 Orion AquaMate Spectrophotometer User Manual ress the A or W key to highlight the desired test and press the enter key Thermo Scientific Thermo Scientific Chapter 8 Absorbance Transmittance and Concentration Measurements Absorbance Ratio The Absorbance Ratio application measures the absorption ratio of two different wavelengths Reference wavelength correction is available to eliminate the effects of a sample matrix Typically used in quality control applications an absorbance ratio provides a convenient and quick diagnostic test for Sample quality This section covers e Setting Up Test Parameters
227. tometer to measure the absorbance of light as it passes through a sample at the 455 nm wavelength This procedure is recommended for water from naturally occurring materials i e vegetable residues such as leaves barks roots humus and peat materials If samples are turbid they should be filtered prior to the analysis trough 0 45 um filter to determine the true color To determine the apparent color non filtered samples are measured Samples with a high color gt 500 PCU should be diluted until the color is within the range of the standard curve Sample pH should not be adjusted as long as It is between 4 and 10 e Pulp Mill Wastewater at 465 nm This method utilizes a spectrophotometer to measure the absorbance of light as it passes through a sample at the 465 nm wavelength This method is adapted trom the NCASI Technical Bulletin 803 for pulp and paper effluent which supersedes NCASI Technical Bulletin 253 The true color is determined in a Sample with the pH adjusted to 7 6 0 05 pH and then passed through a 0 8 um filter The apparent color is determined in the original sample Samples with high color should be diluted to fall within the range of the standard curve There are also multiple wavelength methods which are recommended for color measurement in waters and wastewaters having color characteristics different from but not excluding platinum cobalt standards Such a method is SM 2120D 2001 which is used to calculate Tristimul
228. trip 2 Position the instrument so you can access the power entry module on the back of the instrument 3 Remove the power cord 4 Inserta flat blade screwdriver into the notch on the fuse cover and pry off the cover EY j IAID QA 182 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Thermo Scientific 5 6 1 8 9 Chapter 10 Maintenance Use a flat blade screwdriver to remove the fuse holder Unsnap both fuses to remove them Insert the new fuses pushing them in so they snap into place Replace the fuse cover Replace the power cord 10 Plug the instrument back in to the wall outlet or power strip and turn on the power Note If the fuse blows again contact your distributor or technical support Orion AquaMate Spectrophotometer User Manual 183 Chapter 10 Maintenance Replacing the Tungsten Lamp This procedure is for the Orion AquaMate 7000 Vis spectrophotometer Warning This lamp gets very hot during operation Before removing the lamp turn off the instrument and allow the lamp to cool for 10 minutes Warning Turn off and unplug the instrument before removing the cover of the lamp compartment To replace the tungsten lamp 1 Turn off and unplug the instrument from the wall outlet or power strip 2 Make sure the lamp has cooled for at least 10 minutes 3 Loosen the captive screw on the lamp door by rotating it counterclockwise about 1 4 turn Ali
229. uency of a vial or cuvette the vial or cuvette will break If a vial or cuvette was cleaned in an ultrasonic bath the warranty may be void by the manufacturer Important Do not dry cells in an oven Micro flow cells can be kept clean by e Flushing well with a solvent after use e Aspirating dilute acid base non filming detergent or bleach through the cell in short bursts e Storing with distilled water in the cell Cleaning the Windows of the Sample Compartment Do not use acetone or abrasive materials to clean the windows of the sample compartment Instead use a non abrasive laboratory cleaning solution such as a commercial cell cleaning Solution distilled water or alcohol Use the liquid and a Soft lint free cloth to clean the windows Do not apply too much pressure or the surface of the windows may be damaged Be sure to remove all fingerprints Orion AquaMate Spectrophotometer User Manual 181 Chapter 10 Maintenance Changing the Fuse The fuse is located in the power entry module located at the center of the back panel of the instrument e 120 VAC 2 5A Slo Blo e 240 VAC 1 25 A Slo Blo 2 required Important The instrument fuse must be replaced with the same type and rate Important If the fuse fails repeatedly it may indicate a serious problem with the instrument Contact technical support as soon as possible To change the fuse 1 Turn off and unplug the instrument from the wall outlet or power s
230. ure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Phenol Red Tablet straight from the foil into the vial Crush the tablet with a clean Stir rod 7 Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial 8 Place the vial into the holder in the sample chamber and close the chamber door 9 Press the Measure Sample function key to display the result in pH units Notes Water samples with low values of alkalinity m below 35 mg l CaCO3 may give wrong pH readings OH values below 6 5 and above 8 4 can produce results inside the measuring range A plausibility test 0H meter is recommended The accuracy of the colorimetric determination of pH values depends on various boundary conditions buffer capacity of the sample salt contents etc Salt error Correction of test results average values for samples with salt contents of Salt Content 1 molar 2 molar 3 molar The values of Parson and Douglas 1926 are based on the use of Clark and Lubs buffers 1 Mol NaCl 58 4 g l 5 8 98 Orion AquaMate Spectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC3001 pH Liquid Test Phenol Red Method 6 5 8 4 pH 1 Load and run the AC3001 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vi
231. us Values and ultimately values for the dominant wavelength hue luminance and purity of a sample According to this method the spectrophotometer examines a number of points e g 10 or 30 points in the range from 400 to 700 nm to determine the transmittance at each wavelength and uses the obtained values to calculate color by the published computation method A modification of the Tristimulus Method that Is used in water and waste water industry is based on the measurement of percent transmittance at three wavelengths 590 540 and 438nm gt Range Detection Limit and Sample Cell Size Accuracy precision and detection limits that can be obtained by a specific color measurement procedure depend on the quality of the instrument optics and the sample cell path length e Spectrophotometers allow for more accurate and precise results than colorimeters but are more expensive Lowest detection limits and better accuracy at low levels can be achieved on a spectrophotometer by choosing a sample cell with a longer cell path length Orion AquaMate Spectrophotometer User Manual 115 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate e Colorimeters are useful for testing outside the laboratory and indicate relative color intensity Color values determined on a colorimeter may differ from color values determined on a spectrophotometer especially when the colorimeter wavelength does not match the wavelength used on the spe
232. vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one Alka M Tablet straight from the foil into the vial Crush the tablet with a clean stir rod Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Sample function key to display the result in mg l total alkalinity Notes The terms total alkalinity alkalinity m m value and alkalinity to pH 4 3 are identical For accurate results exactly 10 ml of water Sample must be taken for the test Orion AquaMate Spectrophotometer User Manual 49 Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC3002P Alkalinity P Alkalinity to pH 8 2 Tablet Test Acid Indicator Method ha L 2 Pr PE a 8 9 300 mg l CaCO Load and run the AC 3002P method Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial Place the vial into the holder in the sample chamber and close the chamber door Press the Measure Blank function key to measure the blank Open the sample chamber door and remove the vial from the holder Add one Alka
233. with water free of bromine 10 ml of the diluted sample should be mixed with the reagent and the measurement repeated e Oxidizing agents such as chlorine or ozone interfere as they react in the same way as bromine 58 Orion AquaMate S pectrophotometer User Manual Thermo Scientific Chapter 5 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate AC2017 Chloride Tablet Test Silver Nitrate Turbidity Method 0 5 25 mg l Cl 1 Load and run the AC2017 method 2 Filla clean AQUAfast 24mm round vial Cat No AC2V24 with 10 ml of sample Close the vial tightly with the cap Wipe the exterior of the vial 3 Place the vial into the holder in the sample chamber and close the chamber door 4 Press the Measure Blank function key to measure the blank 5 Open the sample chamber door and remove the vial from the holder 6 Add one Chloride T1 Tablet straight from the foil into the vial Crush the tablet with a clean stir rod 7 Add one Chloride T2 Tablet straight from the foil into the same vial Crush the tablet with a clean stir rod 8 Close the vial tightly with the cap and swirl gently until the tablet is dissolved Wipe the exterior of the vial 9 Wait fora reaction period of 2 minutes 10 Place the vial into the holder in the sample chamber and close the chamber door 11 Press the Measure Sample function key to display the result in mg l chloride Notes Thermo Scientific Ensure that all particles of the tab

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