USB-ME128-System Manual
Contents
1. For MEA or ME Systems Microelectrode Arrays A broad range of microelectrode probes are available from several providers The following are compatible with the miniature preamplifiers from Multi Channel Systems If you are interested in a particular probe that is not mentioned here please ask Multi Channel Systems or your local retailer for compatibility Flexible microelectro de array FlexMEA36 Flexible microelectro de array FlexMEA72 Flexible Eco microelectro de array Eco FlexMEA36 Flexible Eco microelectro de array Eco FlexMEA24 NeuroNexus acute NeuroNexus probes 26 Multi Channel Systems MCS GmbH www multichannel systems com With 36 electrodes in total 32 electrodes arranged in a 6 x 6 grid 2 reference electrodes 2 ground electrodes 30 um electrode diameter 300 um interelectrode distance TiN electrodes and gold tracks embedded in flexible polyimide With 72 electrodes in total 72 electrodes arranged in a 8 x 9 grid 4 reference electrodes 4 ground electrodes 100 um electrode diameter 625 750 um interelectrode distance TiN electrodes and gold tracks embedded in flexible polyimide With 36 electrodes in total 32 electrodes arranged in a 6 x 6 grid 2 reference electrodes 2 ground electrodes 50 um electrode diameter 300 um interelectrode distance Gold electrodes and gold tracks embedded in flexible polyimide Kapton With 36 electrodes in total 24 electrodes arra2. 5 2 5 3 5 4 5 5 Table of Contents Important Safety Advice Important Safety Advice Guarantee and Liability Operator s Obligations Introduction About this Manual Welcome to the USB ME128 System USB ME128 System Setting Up and Connecting USB ME128 System Front Panel 3 2 1 Power LED 3 2 2 Analog Inputs A and B 3 2 3 Digital Trigger Inputs Rear Panel 3 3 1 Digital In Out 3 3 2 Digital Output DO 3 3 3 Synchronization Out and Input 3 3 4 Audio Output 3 3 5 Analog Input 1 to 4 3 3 6 USB 3 3 7 Power Input 3 3 8 Ground 3 3 9 External Power Supply Connecting the USB ME128 System 3 4 1 Connecting the USB ME12864 System to a STG Software Installation 3 5 1 System requirements 3 5 2 Recommended BIOS settings 3 5 3 First Use of MC_Rack Troubleshooting About Troubleshooting Technical Support No Computer Connection No Recording Possible Cable Connection not correct No Recording Possible Triggering Digital Input does not Work Appendix Technical Specifications Pin Layout Contact Information Ordering Information Scope of Delivery Index N N gt Du WwW WN Y Y Y Y Y MV dd O OA U UI U U U U _ O O O 0 13 13 13 14 15 15 17 17 19 21 22 27 29 1 Important Safety Advice 1 1 Important Safety Advice Warning Make sure to read the following advice prior to installation or use of the device and A the software If you do not fulfill all requirements stated below
3. The 68 pin MCS standard connectors on the front panel of the device is an analog input are analog inputs for the data streams coming from the connected amplifiers The connector on the front panel labeled with A and B communicates communicate with the digital Trigger Inputs 0 and 1 The MCS high grade cables C68x1M or C68x3M with 68 pin MCS standard connectors on both heads are used to connect MEA or ME amplifiers to the USB ME128 for analog data transfer Please read chapter Pin Layout 68 pin MCS standard connector in the Appendix Digital Trigger Inputs The Digital Trigger Inputs needs need Lemo coaxial cables with BNC connectors for the MEA amplifier at one head and Lemo connectors at the other head for the USB ME128 device C BNC Lemo The Digital Trigger Inputs accept TTL pulses from any device for example signals from a stimulus generator STG for triggering the system and they control the recording 3 3 3 3 1 3 3 2 3 3 3 A USB ME128 System Manual Rear Panel DIGITAL IN OUT DIG OUT SYNC SYNC AUDIO ANALOG IN Usa Pi DO OUT IN OUT 2 3 a N YO r Y do der ANS N JIA OWY WY e A Digital In Out A Digital IN OUT for 16 digital in and output bits is available 68 pin MCS standard connector The Digital IN OUT connection accepts or generates standard TTL signals TTL stands for Transistor Transistor Logic A TTL pulse is defined as a digital signal for communication between two devices A voltage
4. between 0 V and 0 8 V is considered as a logical state of 0 LOW and a voltage between 2 V and 5 V means 1 HIGH The Digital OUT allows generating a digital signal with up to 16 bits and read it out for example by using a Signal Divider SD16 from MCS You can utilize this digital signal to control and synchronize other devices with the USB ME128 Bit 0 of the Digital OUT is separated and available as Lemo connector DIG OUT DO So if you need only one bit of the digital signal you don t need the additional signal divider SD16 Please read chapter Pin Layout Digital IN OUT Connector in the Appendix for more information The Digital IN can be used to record additional information from external devices as a 16 bit encoded number The Digital IN is most often used to trigger recordings with a TTL signal from a stimulator Therefore the bits 0 1 2 and 3 of the Digital IN are accessible as the Lemo connectors on the front panel The 16 digital input channels are represented as the digital data stream that is a stream of 16 bit values each bit 0 to 15 representing one digital input Standard TTL signals are accepted as input signals on the digital inputs Unused input bits which have an undefined state should be masked in the Trigger Detector of MC_Rack Warning A voltage that is higher than 5 Volts or lower than 0 Volts that is a negative voltage applied to the digital input would destroy the electronics Make sure that you a
5. 2 x MPASI integrated FA16l integrated 16 channel data acquisition USB 2 0 data transfer to any computer and software package Stand alone system for extracellular recordings complete with 1 x MPA32I OR 4 x MPASI integrated FA321 integrated 32 channel data acquisition USB 2 0 data transfer to any computer and software package Complete with 2 x MPASI SC8x8 FA161 data acquisition computer with MC_Card and IPS10W and software package Complete with 2 x MPA32I SC2x32 FA32I data acquisition computer with MC_Card and IPS10W and software package Complete with 2 x MPA32I SC2x32 FA64I data acquisition computer with MC_Card and IPS10W and software package Complete with 4 x MPA32I 2 x SC2x32 2 x FA64l data acquisition computer with MC_Card and IPS10W and software package Complete with 2 x MPASI SC8x8 PGA16 data acquisition computer with MC_Card and IPS10W and software package Complete with 2 x MPA32I SC2x32 PGA32 data acquisition computer with MC_Card and IPS10W and software package Complete with 2 x MPA32I SC2x32 PGA64 data acquisition computer with MC_Card and IPS10W and software package Complete with 4 x MPA321 2 x SC2x32 2 x PGA64 data acquisition computer with MC_Card and IPS10W and software package Appendix ME Amplifiers Product Product Description Number Miniature preamplifier with 2 electrode inputs MPA2I Small sized and light weight 7 7 headstage with common Miniature p
6. Analog Raw Data Electrode Raw Data and Filtered Data can be converted into sound The quality of the sound is mono Note You can add only one sound instrument to your rack and you can convert only one channel into sound Analog Input 1 to 4 Four additional analog inputs are available separated from 68 pin MCS standard connector B on the front panel Pin Number 125 126 127 and 128 See Appendix chapter Pin Layout 68 pin MCS standard connector The MEA1060 amplifier delivers analog data streams from 60 channels to the input connector on the front side of the USB ME128 Pin Number 1 67 and 68 are used for the external power supply Pin Number 2 is for grounding the signal GNDS There are four free channels left Pin No 63 64 65 and 66 that is for Analog IN 1 to 4 The additional analog inputs 1 to 4 are intended for recording additional information from external devices for example for recording patch clamp in parallel to the MEA recording for monitoring the temperature or for recording voice You could also use the analog inputs for triggering but please note that the digital inputs especially DIG OUT DO are intended for accepting TTL pulses As the gain is generally completely different on the electrode inputs and on the additional analog inputs signals on the analog channels are recorded as is with no respect to the gain specified in MC_Rack USB The USB connector is used to transfer the digitized data from al
7. B ME128 Systems but you need a separate data proceeding computer with MC_Rack for each system 3 1 3 2 3 2 1 3 2 2 3 2 3 USB ME128 System Setting Up and Connecting USB ME128 System USB ME128 System is a high flexible system Via MCS high grade cables it is possible to connect for example a MEA60 System or two MEA120 Systems or ME Systems with at least 128 channels to the 128 analog inputs Example The description of the installation includes an example setup with a MEA1060 amplifier two MEA1060 amplifiers without blanking circuit Warning Please read the separate manuals of all devices before installation especially the warnings and safety information Make sure all devices are switched off before you connect them to the power supply Damage to the devices and even fatal injuries may result from improper installation or use Note Using a USB hub for connecting the USB ME128 System to the computer is not recommended The system needs a broad bandwidth for the data transmission Recording might not be possible especially if a second device that sends or receives continuous data streams for example a web cam or USB speakers is connected to the same USB port Front Panel 4 2 USB ME128 System mattone TOMO MOS Analog Input A Digital Trigger IN Analog Input B Power LED The Power LED lights up when the USB ME128 is connected via power supply unit to the power supply system Analog Inputs A and B
8. Data acquisition system with 16 ME16 analog channels System Data acquisition system with 32 ME32 analog channels System Data acquisition system with 64 ME64 analog channels System Data acquisition system with 128 ME128 analog channels System ME recording system with 16 USB ME16 analog channels and filter FAI System amplifier with fixed gain USB ME32 FAI System ME recording system with 32 analog channels and filter amplifier with fixed gain ME16 FA System ME recording system with 16 analog channels and filter amplifier with fixed gain ME32 FA System ME recording system with 32 analog channels and filter amplifier with fixed gain ME64 FA System ME recording system with 64 analog channels and filter amplifier with fixed gain ME128 FA System ME recording system with 128 analog channels and filter amplifier with fixed gain ME16 PGA System ME recording system with 16 analog channels and filter amplifier with programmable gain ME32 PGA System ME recording system with 32 analog channels and filter amplifier with programmable gain ME64 PGA System ME recording system with 64 analog channels and filter amplifier with programmable gain ME recording system with 128 analog channels and filter amplifier PGA with programmable gain 24 Complete with data acquisition computer with MC_Card and IPS10W and software package Stand alone system for extracellular recordings complete with
9. Please note that channels 125 to 128 are not used for recording analog data from MEA amplifier The 68 pin MCS standard connector has 64 possible channels for recording but MEA amplifier only has 60 channels for recording data That is why pin No 63 to 66 are diverged from 68 pin MCS standard connector B at the front panel and connected to the Analog 1 to 4 at the rear panel These channels are used for the additional analog inputs of the USB ME128 Analog 1 Channel 125 Connector B Pin 63 Analog 2 Channel 126 Connector B Pin 64 Analog 3 Channel 127 Connector B Pin 65 Analog 4 Channel 128 Connector B Pin 66 19 USB ME128 System Manual Digital IN OUT Connector 68 Pin MCS Standard Connector 00000 OJOJOJOJO Digital Out DO 20 Pin Pin Pin Pin Pin Pin Pin Pin Pin Pin Pin Pin Pin Pin Bit O of the 16 bit digital output channels Pin 3 1 2 3 10 11 14 15 22 23 26 27 34 35 38 39 46 47 48 49 63 64 66 67 68 GNDP power ground GNDS signal ground Digital output channels bit 0 7 GNDS signal ground Digital output channels bit 8 15 GNDS signal ground Digital input channels bit 0 7 GNDS signal ground Digital input channels bit 8 15 GNDS signal ground Internal use do not connect GNDS signal ground Positive supply voltage output Negative voltage supply output 5 3 Appendix Contact Informati
10. Sync In 1 Sync Out 1 Audio Output 4 Analog Inputs USB Power Supply Ground External Power through Power supply unit MPU 30 Input voltage Output voltage Max Power Software Operating system MC_Rack program Data acquisition and analysis software MC_DataTool program Data export software LTP Director Data acquisition stimulation and analysis software 18 typical 0 01 maximal 0 025 incapable of measurement maximal 2 mV Lemo connector EPL 00 250 NTN 68 pin MCS standard connectors MCS high grade cable 68 pin MCS standard connectors MCS high grade cable Lemo connector EPL 00 250 NTN Lemo connector EPL 00 250 NTN Lemo connector EPL 00 250 NTN Stereo jack 3 5 mm FFA 00 250 CTAC29 Lemo connector EPL 00 250 NTN USB 2 0 High Speed cable type A mini B 5 pin DC PWR 0 7 x 2 35 mm PWR DC 0 65 x 2 75 mm Common jack 4 mm banana plug Lemo EPG 0B 303 HLN 90 264 VAC 47 63 Hz 11 13V 30 W Microsoft Windows 7 XP or Vista with NTFS English and German version supported Version 3 6 3 and higher Version 2 4 1 and higher Axon binary file abf ASCII file txt binary file raw Version 1 2 0 Appendix 5 2 Pin Layout Analog IN Pin Layout 68 Pin MCS Standard Connector 00000 00000 een Galvanically isolated voltage supply and ground Pin 1 67 and 68 are connected to the external power supply for example PS40W Additional ANALOG IN
11. ata acquisition computer with MC_Card and IPS10W MEA1060 Inv amplifier TC01 ALA MEA PPORT2 and accessories Complete with 5 MEAs data acquisition computer with MC_Card and IPS10W MEA1060 Up amplifier TCO1 ALA MEA PPORT2 and accessories Complete with 5 MEAs data acquisition computer with MC_Card and IPS10W MEA1060 Inv amplifier TCO2 PHO1 ALA MEA PPORT2 and accessories Complete with 5 MEAs data acquisition computer with MC_Card and IPS10W MEA1060 Up amplifier TC02 PHO1 ALA MEA PPORT2 and accessories Complete with 5 MEAs data acquisition computer with MC_Card and IPS10W 2 x MEA1060 Inv amplifier TCO2 ALA MEA PPORT2 and accessories Complete with 5 MEAs data acquisition computer with MC_Card and IPS10W 2 x MEA1060 Up amplifier TCO2 ALA MEA PPORT2 and accessories Complete with 5 x 256MEAs data acquisition data conversion and filter amplification 252 recording channels 4 additional analog channels 1 digital I O port and USB 2 0 High Speed data transfer to any computer Appendix MEA Amplifiers Product Product Description Number MEA amplifier for inverted MEA1060 Probe interface and 60 channel pre and microscopes Inv filter amplifier with custom gain and bandwidth MEA amplifier for upright MEA1060 microscopes Up MEA amplifier with blanking MEA1060 Probe interface and 60 channel pre and circuit for inverted microscopes Inv BC filter amplifier with custom gain and bandwid
12. ation or maintenance of the device Unauthorized structural alterations to the device Unauthorized modifications to the system settings Inadequate monitoring of device components subject to wear Improperly executed and unauthorized repairs Unauthorized opening of the device or its components Catastrophic events due to the effect of foreign bodies or acts of God Operator s Obligations The operator is obliged to allow only persons to work on the device who are familiar with the safety at work and accident prevention regulations and have been instructed how to use the device are professionally qualified or have specialist knowledge and training and have received instruction in the use of the device have read and understood the chapter on safety and the warning instructions in this manual and confirmed this with their signature It must be monitored at regular intervals that the operating personnel are working safely Personnel still undergoing training may only work on the device under the supervision of an experienced person 2 1 Introduction About this Manual It is assumed that you already have a basic understanding of technical and software terms No special skills are required to read this manual If you are using the device for the first time please read the Important Safety Advice before installing the hardware and software where you will find important information about the installation and first steps The devi
13. ations in the background when using MC_Rack Remove all applications from the Autostart folder Be careful when using a Virus Scanner These programs are known to disturb MC_Rack and even data loss may occur When using an USB ME128 System it is recommended to connect a high performance computer with a separate hard disk for program files and data storage The provided possibility to use up to 128 channels with up to 40 kHz needs high memory capacity Please remove data and defragment the hard disk regularly to ensure optimal performance 10 3 5 3 USB ME128 System First Use of MC_Rack It is also not recommended to run any applications in the background when using MC_Rack Remove all applications from the Autostart folder Warning The operating system settings of the data acquisition computer were pre configured by MCS and should not be changed by the user Changing these settings can lead to program instabilities and data loss Installing MC_Rack with the USB ME128 System connected to the computer The Windows operating system detects a new hardware when the USB ME128 System is connected to the computer if the program has not been installed beforehand Please make sure the device has power that is the power LED is lighting Simply cancel the Found New Hardware Wizard and proceed with the installation of the MC_Rack program Connecting the USB ME128 System to a computer with installed MC_Rack If MC_Rack had already been ins
14. ce and the software are part of an ongoing developmental process Please understand that the provided documentation is not always up to date The latest information can be found in the Help Check also the MCS web site www multichannelsystems com for downloading up to date manuals and help files USB ME128 System Manual 2 2 Welcome to the USB ME128 System system ysB ME128 I The USB ME128 System is a stand alone solution for acquiring data from up to 128 channels It can replace the internal computer hardware MC_Card The analog input signals are acquired and digitized by the USB ME128 System and the digital electrode signals are transmitted to the connected computer via universal serial bus High Speed USB 2 0 Thus it is possible to use any computer for data processing Recorded data are graphed analyzed and reviewed with the powerful and easy to use MC_Rack program You can export the data in standard formats to other programs with the software MC_DataTool The USB ME128 System supports 128 channels with an amplification factor 1 that means no amplification data acquisition only It is a high flexible system You can connect for example two MEA60 Systems via MCS high grade cables or one MEA120 Systems or ME Systems in different configurations or whatever comes with 68 pin MCS standard connectors Please see Microelectrode Array Systems and Microelectrode Array USB Systems for more information You can stack several US
15. fiers ii 23 MEA SySteMS i 23 ME SystemS n ne a 23 Microelectrode Arrays 23 Spare partS i 23 P Pin Layout 20 Additional Analog IN 20 Analog IN 20 Digital In Out 20 Digital Out DO 20 PS40W 5 R Rear Panel 5 Analog INPUt 5 Audio OUtput 5 Digital In OUt 5 Digital Output DO 5 External Power Supply 5 Ground e aa 5 Power Input 5 Synch OUT IN 5 USB hill ua 5 Recommended BIOS settings 9 S Setup exe 9 Software Installation 9 Recommended Bios Settings 9 System Requirements 9 Spare parts 23 Stimulus Generator 5 System Requirement 9 T Technical Support 13 Triggering Digital Input Does not Work 13 Troubleshooting 13 U User forum 22 31 32
16. have been reported by users Most problems occur seldom and only under specific circumstances Please check the mentioned possible causes carefully when you have any trouble with the product In most cases it is only a minor problem that can be easily avoided or solved If the problem persists please contact your local retailer The highly qualified staff will be glad to help you Please inform your local retailer as well if other problems that are not mentioned in this documentation occur even if you have solved the problem on your own This helps other users and it helps MCS to optimize the instrument and the documentation Please pay attention to the safety and service information in the separate user manuals of the related products and in the software help Multi Channel Systems has put all effort into making the product fully stable and reliable but like all high performance products it has to be handled with care Technical Support Please read the chapter Troubleshooting of the user manual first Most problems are caused by minor handling errors Contact your local retailer immediately if the cause of trouble remains unclear Please understand that information on your hardware and software configuration is necessary to analyze and finally solve the problem you encounter Please keep information on the following at hand Description of the error the error message text or any other useful information and of the context in which the er
17. ital Input does not Work You have connected a TTL source for example the Sync Out of a stimulus generator to the digital output DO of the USB ME128 System and configured the virtual rack in MC_Rack for triggering displays or data acquisition by the TTL source but you do not see any sweeps Possible causes The TTL source does not generate true TTL signals 5 V TTL level or the TTL pulse duration is too short in combination with the sampling rate so that the pulse is missed in between two data points The USB ME128 System can only accept TTL signals 5 V TTL level as a digital input stream The TTL pulse needs to be optimized according to the sampling rate Otherwise a detection of the trigger cannot be guaranteed The software settings for the Trigger Detector do not match with the hardware configuration In MC_Rack add a Trigger Detector to your virtual rack and select the Digital Data D1 input stream as the Trigger Check the pin layout of the digital IN OUT connector and make sure that the same bit input that is connected is selected in the software The standard settings of the Trigger Detector are for using bit 0 Mask all unused bits Select the appropriate logical state generally HIGH for triggering Please see the MC_Rack help or manual for more details 15 5 1 Appendix Technical Specifications The USB ME128 System is an analog digital board converting analog signals in digital data streams in real
18. l data channels and the additional digital and analog channels to any connected data acquisition computer via USB High Speed 2 0 type A mini B cable Power Input Connect here the power supply unit This power supply powers the USB ME128 main unit only The device needs 12 V and Ground If an additional ground connection is needed you can connect this plug with an external ground using a standard common jack 4 mm External Power Supply External Power Supply Plug in for the external power supply for example PS40W The external power supply energizes the devices that are connected to the USB ME128 for example filter amplifier gain amplifier or MEA amplifier with blanking circuit The conductive path from the three pins of the plug in runs through the device to the front 68 pin MCS standard connector and occupies Pin Number 1 67 and 68 Please see also chapter Pin layout 68 pin MCS standard connector in the Appendix USB ME128 System Manual The PS40W is a standard power supply delivering supply voltages to connected devices The PS40W is especially recommended for use with Multi Channel Systems products for example it is perfect for supplying power to MEA1060BC or to programmable gain amplifiers PGA and for other custom setups that lack an internal power supply IPS10W It supplies power with 7 V output voltage 2 5 A output current and about 40 W output power The PS40W stabilizes two voltages using the linear reg
19. mage Damaged leads should be replaced immediately and may never be reused e Do not try to insert anything sharp or metallic into the vents or the case e Liquids may cause short circuits or other damage Always keep the device and the power cords dry Do not handle it with wet hands Requirements for the installation e Make sure that the device is not exposed to direct sunlight Do not place anything on top of the device and do not place it on top of another heat producing device so that the air can circulate freely 1 2 1 3 USB ME128 System Manual Guarantee and Liability The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply The operator will receive these no later than on conclusion of the contract Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software It is up to the user to use good laboratory practice to establish the validity of his her findings Guarantee and liability claims in the event of injury or material damage are excluded when they are the result of one of the following Improper use of the device Improper installation commissioning operation or maintenance of the device Operating the device when the safety and protective devices are defective and or inoperable Non observance of the instructions in the manual with regard to transport storage installation commissioning oper
20. multichannel systems USB ME128 System Manual n 0 0 v 6 eeeseee Information in this document is subject to change without notice No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH While every precaution has been taken in the preparation of this document the publisher and the author assume no responsibility for errors or omissions or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document O 2012 Multi Channel Systems MCS GmbH All rights reserved Printed 23 05 2012 Multi Channel Systems MCS GmbH AspenhaustraBe 21 72770 Reutlingen Germany Fon 49 71 21 90 925 0 Fax 49 71 21 90 92 5 11 info multichannelsystems com www multichannelsystems com Microsoft and Windows are registered trademarks of Microsoft Corporation Products that are referred to in this document may be either trademarks and or registered trademarks of their respective holders and should be noted as such The publisher and the author make no claim to these trademark 1 1 1 2 1 3 2 1 2 2 3 1 3 2 3 3 3 4 3 5 4 1 4 2 4 3 4 4 4 5 5 1
21. nged in a 2 x 10 4 grid 2 reference electrodes 2 ground electrodes 80 um electrode diameter 300 um interelectrode distance Gold electrodes and gold tracks embedded in flexible polyimide Kapton NeuroNexus Technologies www neuronexus tech com 16 32 64 channel silicon probes Appendix 5 5 Scope of Delivery Data acquisition and analysis software MC_Rack Version 3 6 3 or higher Data export software MC_DataTool Version 2 4 1 or higher USB ME128 manual USB ME128 data sheet i 27 6 Index 29 USB ME128 System Manual 30 A Accessories B Bios settings C Connecting the System Connecting with a STG Contact Information Contact Pin Defective F Front Panel Analog INputs Digital Trigger Inputs Power LED G Guarantee and Liability I Important Safety Advice Installing MC_Rack L Lemo Connector Local retailer M Mailing list MC_Card MC_DataTool MC_Rack First Use of MC_Rack Installing MC_Rack MC_Rack manual MC_Rack program MCS high grade cable MEA amplifiers ME amplifiers ME Systems Microelectrode Array Microsoft Windows No Computer Connection No Recording Possible 23 23 23 23 23 13 13 O Operator s Obligations 2 Ordering Information 23 ACCESSOFIES coccoccccconcccnononncocnnnnncnnnannnos 23 ME amplifiers iii 23 MEA ampli
22. nnections If this does not solve the problem contact your local retailer for support The power LED is lighting The USB port might not support USB 2 0 or might not be working Check the USB port Only full speed USB 2 0 ports can be used Try another USB 2 0 port You can establish a connection but get an error message when starting the recording in MC_Rack The bandwidth of the USB port is not sufficient for recording This can be the case if the USB ME128 System is connected via USB hub and a second device that sends or receives continuous data streams for example a web cam or USB speakers is connected to the same USB port Connect the USB ME128 System directly to a USB port not via hub 14 4 4 4 5 Troubleshooting Cable Connection not correct No Recording Possible You cannot establish a signal from the amplifier The USB ME128 System channel layout in MC_Rack shows 50 Hz hum only Possible causes The cable connection from the computer to the periphery is not complete 68 pin MCS standard connectors need to be fixed in the provided plugs by a firm connection Otherwise no data transfer is possible Check the MCS high grade cable and the 68 pin MCS standard connectors If this does not solve the problem contact your local retailer for support The cable connection from the computer to the periphery is not correct Make sure not to mistake inputs for outputs in the connected devices Triggering Dig
23. ode A computer with low performance may lead to performance limits more often therefore MCS recommends an up to date computer optionally with a separate hard disk Please note that there are sometimes hardware incompatibilities of the USB ME128 System and computer components or that an inappropriate computer power supply may lead to artifact signals Please contact your local retailer for more information on recommended computer hardware Important You need to have installed the latest USB ME128 System driver to operate the USB ME128 System which is automatically installed with MC_Rack The installation may be invalid if the USB ME128 System does not respond Please contact Multi Channel Systems or your local retailer in this case Recommended BIOS settings Recommended operating system settings The following automatic services of the Windows operating system interfere with the data storage on the hard disk and can lead to severe performance limits in MC_Rack These routines were designed for use on office computers but are not very useful for a data acquisition computer Turned off Screensaver Turned off Windows Indexing Service for all local disks Turned off Windows System Restore Turned off Optimize hard disk when idle automatic disk fragmentation Manual management of automatic Windows Update Power Options Power scheme Never turned off monitor hard disk and system standby It is also not recommended to run any applic
24. on Local retailer Please see the list of official MCS distributors on the MCS web site User forum The Multi Channel Systems User Forum provides the opportunity for you to exchange your experience or thoughts with other users worldwide Mailing list If you have subscribed to the mailing list you will be automatically informed about new software releases upcoming events and other news on the product line You can subscribe to the list on the contact form of the MCS web site www multichannelsystems com 21 5 4 USB ME128 System Manual Ordering Information Please contact your local retailer for pricing and ordering information MEA Systems Product Product Description Number MEA recording system for inverted microscopes 60 electrode channels MEA recording system for upright microscopes 60 electrode channels MEA recording system for inverted microscopes with advanced perfusion 60 electrode channels MEA recording system for upright microscopes with advanced perfusion 60 electrode channels MEA recording system for inverted microscopes 120 electrode channels MEA recording system for upright microscopes 120 electrode channels USB MEA recording system for inverted and upright microscopes 256 electrode channels 22 MEA60 Inv System MEA60 Up System MEA60 Inv SystemE MEA60 Up SystemE MEA120 Inv System MEA120 Up System USB MEA256 System Complete with 5 MEAs d
25. plifiers with MCS High Grade cables C68x1M to the 68 pin MCS standard connectors A and B on the front panel of the USB ME128 Connect the USB output connector to a free USB 2 0 port of the data acquisition computer It is not recommended to use an USB hub Connect the USB ME128 via power supply unit to a power outlet of the same electrical system connected to the same ground earth wire as all other components of the setup for example the computer or shielding Ground the system Check the power LED on the front panel It should light up as soon as the power line is connected If not check the power source and cabling Install the MC_Rack program from the installation volume if it is not already installed Start the MC_Rack program and select the USB ME128 System as the data source Please see the MC_Rack help manual for more details on how to define the data source Connecting the USB ME128 System to a STG Connect the MEA amplifiers with two MCS high grade cables C68x1M to the 68 pin MCS standard connectors A and B on the front panel of the USB ME128 Connect a Sync Out of a stimulus generator STG via T connector at one hand to the BNC connector of the MEA amplifiers and at the other hand to the Digital Trigger IN 0 to 1 at the front panel of the USB ME128 device Make sure that 68 pin MCS standard connector A of MEA amplifier A communicates with Digital Trigger IN 0 MEA amplifier B with 1 Please see the following
26. pply only TTL pulses 0 to 5 V to the digital inputs Digital Output DO The Bit 0 of the Digital OUT is also accessible independently from the 68 pin Digital IN OUT connector The digital output channel DO is generally used for synchronizing the USB ME128 System with a stimulus generator or with another data acquisition system for example an imaging or a patch clamp system The DIG OUT DO generates standard TTL pulses Synchronization Out and Input If you want to use several USB ME128 Systems in parallel the SYNC OUT and SYNC IN can be used to synchronize the individual devices The SYNC OUT of the first device must be connected to the SYNC IN of the second and so on For the connection you need cable with Lemo plugs on both heads C Lemo Lemo Warning Do not use the SYNC IN and SYNC OUT to connect the USB ME128 to any other device than another USB ME128 3 3 4 3 3 5 3 3 6 3 3 7 3 3 8 3 3 9 USB ME128 System Audio Output To the AUDIO OUT 3 5 mm phone jack you can connect an audio system to make the electrical activity audible By software control it is possible to convert signals from one data channel into sound A computer with a sound card and speakers or a headset is required You can use this feature to hear Electrode Raw Data for example or to replay recorded sound Or you can use an analog channel as a sound input and record any sounds Thus you can add spoken commentary to document your data Only
27. reamplifier with 8 electrode inputs MPASI ground and additional Miniature preamplifier with 32 electrode MPA32I indifferent reference inputs electrode input input type l gain 10 Filter amplifiers with 4 8 16 32 48 or 64 NN is the total number of channels and input type S or channels X is the input type S or I with custom gain and bandwidth Amplifier with programmable gain 16 PGA16 Gain programmable from channels 10 to 5000 Amplifier with programmable gain 32 PGA32 channels Amplifier with programmable gain 64 PGA64 channels Amplifier with programmable gain 16 input PGA1632 Gain programmable from and 32 output channels 10 to 5000 with two Amplifier with programmable gain 32 input PGA3264 different pass bands and 64 output channels Accessories Product Product Description Number Power supply For supplying power to programmable gain amplifiers with 40 W power for custom setups that lack an internal power supply and 7 V output or for other custom applications voltage 32 Channel ADPT CN 32 Allows a direct connection of electrodes with cactus Cactus Needle needles to a 32 channel miniature preamplifier MPA321 Adapter NeuroNexus ADPT NN 16 The 16 Electrode NeuroNexus Probe Adapter allows Probe adapters ADPT NN 32 a direct connection of 16 Electrode acute probes from for 16 32 or 54 ADPT NN 54 NeuroNexus Technologies to two 8 channel miniature channels preamplifiers MPABI The 32 Electrode Neu
28. roNexus Probe Adapter allows a direct connection of 32 Electrode acute probes from NeuroNexus Technologies to a 32 channel miniature preamplifier MPA321 The 54 Electrode NeuroNexus Probe Adapter allows a direct connection of 54 Electrode acute probes from NeuroNexus Technologies to two 32 channel miniature preamplifiers MPA321 Signal collector Collects the 8 channels from each of the up to for 8 x MPASI 8 miniature preamplifiers MPA8I and leads them to the amplifier via a 68 pin MCS standard cable Signal collector SC2x32 Collects the 32 channels from each of the up to for 2 x MPA32I 2 miniature preamplifiers MPA32I and leads them to the amplifier via a 68 pin MCS standard cable 25 USB ME128 System Manual SC8x8BC SD16 SD32 SD64 Signal collector with blanking circuit for 8 x MPASI Collects the 8 channels from each of the up to 8 miniature preamplifiers MPA8I and leads them to the amplifier via a 68 pin MCS standard cable the voltage outputs are held constant during the TTL blanking signal stimulus artifacts are avoided Signal dividers with 16 32 or 64 channels Placed between amplifier and MC_Card permits to select any channel does not interfere with the data acquisition Signal grounding unit For silencing defective electrodes Single channels can be switched off with small toggle switches Inputs not in use are grounded Spare parts Product Product Number 0 5 m 68 Pin MCS Standard cable
29. ror occurred Try to remember all steps you had performed immediately before the error occurred The more information on the actual situation you can provide the easier it is to track the problem The serial number of the device You will find it on the backside of the housing or click About in Help in the main menu of MC_Rack The amplifier type and serial numbers of the connected amplifiers You will find them on the devices The operating system and service pack number of the connected computer The hardware configuration microprocessor frequency main memory hard disk of the connected computer This information is especially important if you have modified the computer or installed new hard or software recently The version of the recording software On the Help menu click About to display the software version 13 4 3 USB ME128 System Manual No Computer Connection No Recording Possible You cannot establish a connection to the computer The USB ME128 System channel layout is not available in MC_Rack When loading a previously saved virtual rack file you will get an error message and the simulator will be started automatically Or you get an error message when starting the recording in MC_Rack after a successful computer connection Possible causes The power LED is not lightning The supply power is not connected or there is a technical problem with the instrument Check the power source and the cable co
30. suggestion BNC connector USB High Grade cable T connector Trigger In Front Panel MEA Amplifier USB ME Main Unit RS232 connector USB High Grade cable Data Acquisition Serial Port Computer Port 3 5 3 5 1 3 5 2 USB ME128 System Manual Software Installation Please check the system requirements before you install the MC_Rack software MCS cannot guarantee that the software works properly if these requirements are not fulfilled Please see the MC_Rack help or manual for more information It is recommended that you check the MCS web site for software updates on a regular basis The USB ME128 System is a plug and play device The driver is automatically installed together with the MC_Rack program It is easier to connect the USB ME128 System first to the data acquisition computer and then install MC_Rack Important Please make sure that you have full control over your computer as an administrator Otherwise it is possible that the installed hardware does not work properly System requirements One of the following Microsoft Windows operating systems is required Microsoft Windows 7 VISTA or XP English and German versions supported with the NT file system Other language versions may lead to software errors Hardware Not required for offline analysis or demo mode The data acquisition board USB ME128 System If no USB ME128 System is present MC_Rack opens in a simulation m
31. talled on the computer before the hardware was connected the operating system needs to load the driver once After this procedure the USB ME128 System will be automatically recognized by the operating system Connect the USB ME128 System to the USB port of the data acquisition computer Please make sure the device has power that is the power LED is lighting Switch the computer on The Found New Hardware Wizard is displayed Choose the option No not this time and continue with Next Choose the option Install the software automatically Recommended and continue with Next The Wizard will automatically find the appropriate USB ME128 System driver Continue with Next The driver installation is finished Please check the system requirements before you install the MC_Rack software MCS cannot guarantee that the software works properly if these requirements are not fulfilled Please see the MC_Rack Help or Manual for more information It is recommended that you check the MCS web site for software updates on a regular basis Double click Setup exe on the installation volume The installation assistant will show up and guide you through the installation procedure Follow the instructions of the installation assistant The USB ME128 System driver and MC_Rack are installed or updated automatically 11 4 1 4 2 Troubleshooting About Troubleshooting The following hints are provided to solve special problems that
32. th The blanking circuit prevents MEA amplifier with blanking MEA1060 the amplifier from getting saturated and circuit for upright microscopes Up BC thus prevents stimulus artifacts Accessories Product Product Description Number CCIR Suitable for all MEAs with plastic ring and thread Simply screw culture the culture chamber onto the plastic holder on the MEA An O chamber ring ensures that the chamber fits tightly and is leakproof Autoclavable Complete with lid MEA Additional or replacement lid for MEA culture chamber culture chamber lid Sealed MEA culture chamber with transparent semi permeable MEA membrane suitable for all MEAs with glass ring Simply slide culture the culture chamber over the glass ring A hydrophobic semi dish permeable foil from Dupont that is selectively permeable to gases 02 CO2 but not to fluid keeps your culture clean and sterile preventing contaminations by airborne pathogens It also greatly reduces evaporation and thus prevents a dry out of the culture Autoclavable Comes complete with membranes Set of 10 membranes for sealed MEA culture dishes Set of 5 membranes and MEA MEM TOOL for sealed MEA culture dishes Tool for smoothing and positioning the membrane on the ALA MEA MEM culture chamber so that the membrane is neat and flat on top of the culture chamber perfusion insert INSERT 23 USB ME128 System Manual ME Systems Product Product Description Number
33. this may lead to malfunctions or breakage of connected hardware or even fatal injuries Warning Always obey the rules of local regulations and laws Only qualified personnel should A be allowed to perform laboratory work Work according to good laboratory practice to obtain best results and to minimize risks The product has been built to the state of the art and in accordance with recognized safety engineering rules The device may only e be used for its intended purpose e be used when in a perfect condition e Improper use could lead to serious even fatal injuries to the user or third parties and damage to the device itself or other material damage Warning The device and the software are not intended for medical uses and must not be used D on humans Malfunctions which could impair safety should be rectified immediately High Voltage Electrical cords must be properly laid and installed The length and quality of the cords must be in accordance with local provisions Only qualified technicians may work on the electrical system It is essential that the accident prevention regulations and those of the employers liability associations are observed e Each time before starting up make sure that the power supply agrees with the specifications of the product e Check the power cord for damage each time the site is changed Damaged power cords should be replaced immediately and may never be reused e Check the leads for da
34. time Warning The device may only be used together with MEA Systems or ME Systems from Multi Channel Systems MCS GmbH and only for the specified purpose Damage of the device and even fatal injuries can result from improper use Do not open the data acquisition box and do not change hardware configuration as it could lead to improper behavior of the system General characteristics Operating temperature Storage temperature Relative humidity Dimensions L x D x H Weight Signal inputs Number of analog recording channels Input voltage Input impedance Bandwidth Number of additional analog inputs Input voltage range Bandwidth Input impedance DC offset Number of digital input channels Digital input signals Number of digital output channels Digital output signals Sampling frequency Maximal data rate Data resolution Data transfer 10 C to 50 C 0 C to 50 C 10 to 85 non condensing 210 x 210 x 50 mm 1850 g 128 AV 10kQ DC to 13 kHz 4 4 096 V DC to 15 kHz 1 MQ 1 nF max 2 mV 16 CMOS 3 3 V input 16 TTL 3 3 V output up to 50 kHz software controlled 20 5 MB s 16 Bit USB 2 0 High Speed true USB 2 0 transfer rate 17 USB ME128 System Manual Cross talk neighbour channel Cross talk other channels DC offset Interface and connectors Front panel 42 Digital Inputs 42 x 64 Analog In Rear panel 1 16 Bit Digital In Out 1 Digital Out DO 1
35. ulation technique The outputs of the device have over current and over temperature protection In case of a failure of the PS40W the output voltages are decreased Fuses protect the electronic components against fatal damages Important Do not mismatch the polarity of the power supply A false connection may cause permanent damages Note If by any cause the supply voltages are reduced the recorded signals could be deformed device in experimental setup The PS40W will disturb the data acquisition with 50 Hz hum N Please do not place the external power supply device PS40W near to the data acquisition that is why the PS40W should be placed in a distance to the data acquisition device Cabling For Digital Out DO and Analog Input 1 to 4 on the rear panel of the device you need the same special coaxial cable as for the Digital Trigger In plugs on the front panel Lemo cable with one head BNC connector the other head Lemo connector Please see picture above Sync In and Sync Out need Lemo cable with two Lemo connectors 3 4 3 4 1 USB ME128 System Connecting the USB ME128 System Provide a power supply in the immediate vicinity of the installation site Place all devices on a stable and dry surface where the air can circulate freely and the devices are not exposed to direct sunlight Set up the computer with installed MC_Rack program Set up the MEA1060 amplifiers as described in the MEA1060 Manual Connect the MEA am
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