tacoTM Instrument user manual
Contents
1. After the extraction the taco is not reset The door of taco is open TM During the taco extraction process 11 4 5 How to shut down the taco 4 5 1 Before switching off the taco please make sure the plates and sleeves are removed from the taco 4 5 2 Press the door button to close the door of the taco 4 5 3 Turn off the main switch at the rear of the taco to shut down 12 5 Cleaning and Maintenance 5 1 General Care Do not disassemble the taco for cleaning This will void the warranty Never allow liquids to sit on the extraction system surfaces for extended periods of time Do not use solvent detergent or bleach to clean the magnetic rods Use only clean and wet paper towel to wipe out stains on magnetic rods Note Wear gloves or other protective equipment Always turn off and unplug the taco before cleaning 5 2 Dealing with Spills If case of liquid spills please contact your local distributor or GeneReach for assistance Do not dismantle the instrument for cleaning Please make sure the total volume of each well does not exceed 900 ul before extraction Wipe out visible stains with disposable paper towel immediately See Troubleshooting Using UV light for sterilization after each extraction is recommended 13 Troubleshooting taco does not perform normally a The LED light does not go up b Self test runs overtime c The upper2. taco Nucleic Acid Automatic Extraction System 4 Operation Procedure of taco 4 1 How to START the taco 4 1 1 Load the reagent to 96 Well Extraction Plate in advance See taco nucleic acid extraction kit manuals for instruction 4 1 2 Plug in the taco and switch on the main power the device will complete self check within 3 minutes The machine is ready when the green LED lights up 4 1 3 Press the door button to open the door of taco load 96 Well Extraction Plate and Mixing Sleeve according to the instructions See chapter 2 6 Loading Plate amp Sleeve 4 1 4 Push the Plate amp Sleeve to the bottom of the plate holder and lower tray Ensure the Plate amp Sleeve are in the correct position 4 1 5 Press the door button of the taco to close the door 4 1 6 Press START to begin the extraction Note The operating temperature should be under room temperature 16 30 C Note Under these circumstances listed below the START function is nonfunctional The UV Lamp is on While the door of taco is open 4 2 How to PAUSE the taco 4 2 1 During the extraction press the PAUSE button to stop the taco if necessary The yellow LED will flash under pause status 4 2 2 The magnetic rods and Mixing Sleeves will move up to the top together 4 2 3 Close the door of taco and press Start Resume to resume the extraction Note Under these circumstances
3. tray and lower tray are not in correct position d Noises from the machine e Liquid spilled and adhered to device Magnetic rod function failure a Stain on the magnetic rod b The magnetic rod comes off from the heat shrink sleeve Comments and Suggestions e Check the plug to see if it has been connected to the power system e Check the main power to see if it has been switched on e Measure the voltage of electricity outlet e Contact your local distributors or GeneReach for assistance e Contact your local distributors or GeneReach for assistance e Contact your local distributors or GeneReach for assistance e Check the Mixing Sleeves to see if they have been pushed to the bottom of lower tray e Check the 96 Well Extraction Plate to see if it has been pushed to the bottom of the plate holder e Check that there is nothing preventing the track from moving e Contact your local distributor or GeneReach e The total volume of each well should not exceed 900 ul e Use UV light for sterilization and then clean the taco with water or laboratory detergent Repeat the extraction procedures with new samples and reagents e Ensure the Mixing Sleeves are installed before each extraction e Use clean and wet paper towel to wipe it out carefully Do not use any solvent or detergent to clean the magnetic rods e Contact your local distributor or GeneReach 14 Comments and Sugges
4. washing and eluting steps The taco is intended to be used for extracting nucleic acid for research use purpose by using corresponding nucleic acid extraction kit developed by GeneReach Biotechnology Corporation GeneReach 1 1 Intended Use The taco is intended to extract nucleic acid from nucleic acid containing solutions for research purpose using the corresponding extraction kits developed by GeneReach through the automatic transfer and processing of magnetic bead This product is intended to be used by professional users such as well trained laboratory technicians familiar with molecular biology techniques It is not intended to be used for any therapeutic or diagnostic purpose 1 2 taco Extraction Principle The taco is based on the magnetic separation technology with the special design of the disposable taco Plate amp Sleeve which is provided with the reagent kit Samples and reagents including the magnetic particles are dispensed into the wells according to the corresponding kit instructions The automated steps performed by the taco include Magnetic Bead is transferred and mixed with samples Samples are lysed and nucleic acid is bound to Magnetic Bead The impurity is washed away from the nucleic acid is bound to Magnetic Bead Nucleic acid is eluted from the Magnetic Bead taco Nucleic Acid Automatic Extraction System 2 taco Layout 2 1 Product Components Component
5. Quantity l taco Power Cord User Manual Warranty Card 2 2 taco Specifications Specification Dimensions W x D x H 42 cm x 30 cm x 38 cm Net Weight 23 4 kg 100 120 200 240V AC 50 60 Hz 2A T Operating Temperature 16 30 C Through put at leastl6 Samples Power Requirement 2 3 Equipments to Be Supplied by User taco nucleic acid extraction kit taco Plate amp Sleeve Uninterrupted power system UPS optional Laminar air flow optional Disposable gloves Protective goggles Lab coat Note To avoid power failure connecting the taco to an UPS is recommended taco Nucleic Acid Automatic Extraction System 2 4 The Front View of the taco 2 4 1 LED Signal fun Stand by Run Stand by Signal Lights Up Self test passed Ready to run Pause End Signal Lights Up Extraction finished P UV On Signal Lights U UV Lamp on Green LED Error Signal Light UP Any Error occurred Red LED taco Nucleic Acid Automatic Extraction System 2 4 2 Operation Button Pause Reset Function disabled under the No Button Description following status During the extraction 1 Door Press to open close the door UV Lamp is on During Magnetic Bead transfer Short press to pause the During Magnetic Bead drying machine UV Lamp is on 2 Pause Reset While paused or extraction Reset is functional only under pause or finished long press to reset extr
6. action finished status the machine Press to start resume the 1 Door is open 3 Start machine 2 UV Lamp is on taco Nucleic Acid Automatic Extraction System 2 5 The Rear View of the taco r pb UV light switch To turn on off the UV light taco Nucleic Acid Automatic Extraction System 2 6 Loading taco Plate amp Sleeve 2 6 1 Loading the 96 Well Extraction Plate Protection of magnetic sticks and mixing the sample 1 Mixing Sleeve with Magnetic Bead Deep well plate for extraction Use two Mixing Sleeves 96 Well Extraction Plate for each 96 Well Extraction Plate when processing extraction s Loweriay Mowing torte Mining Steve Note The Mixing Sleeves and the 96 Well Extraction Plate should be placed on the same side when processing extraction See illustrations above Inadequate installation of taco Plate amp Sleeve may damage the machine taco Nucleic Acid Automatic Extraction System 2 6 2 Use of Mixing Sleeve Mixing Sleeve Mortise Hook of Mixing Sleeve Lift up the hook to tenon the mortise Button of Mixing Sleeve Press the button to remove Mixing Sleeve Note Using non recommended consumables may affect the device performance taco Nucleic Acid Automatic Extraction System 3 Important notes After receiving the taco please check the instrument and accessories for damage Should any question occurred please contact your l
7. listed below the Pause will be nonfunctional During the drying and transferring process of Magnetic Bead The UV Lamp is on 10 4 3 How to RESET the taco 4 3 1 After the extraction is finished the yellow LED will goes up steadily together with a long beep sound 4 3 2 Long press the DOOR button to stop the alarm and the door will be opened 4 3 3 Take out the 96 Well Extraction Plate the extracted nucleic acid is ready for immediate use 4 3 4 To reset the taco long press the PAUSE RESET button 4 3 5 After a short beep sound the taco is reset and ready when the green LED lights up Note Please take out the extracted nucleic acid immediately after extraction otherwise it may not perform well in downstream applications Note Under these circumstances listed below the Reset function will be nonfunctional The Reset function will only be FUNCTIONAL under pause status or when extraction finished The UV Lamp is on 4 4 How to Use the UV Lamp 4 4 1 After the extraction is finished remove the Plate amp Sleeve and press the RESET button 4 4 2 Press the UV light switch at the rear of taco the sterilization will start automatically 4 4 3 The sterilization will continue for 30 minutes To manually stop the UV Lamp press the UV light switch again Note Under the circumstances listed below the UV Lamp function will be nonfunctional
8. necuecseesneeseeeneeneestees 10 4 3 How to RESET the taco ccccceccessssssesseessesseessecsessnecseesnecuecsnseuecsessecseesnecuecsessneeseseneeneestes 11 4A Howto Use the UV LAMP enecens EEE aE EAE EE ER E EE NOR l1 4 5 How to shut down the taco iceccccccccsssscsessssssssesesesscscevscsesecscavevsesesesacavstsestsssavevstseseeees 12 S Cleanine and Mantenan siess eresio aa EEES E E 10 SAEC EO 1 EE ee ee E E E N 13 SPAD EE o l E E E E A anctceesusvenshee 13 Touple OO UNA e E E S 14 taco Nucleic Acid Automatic Extraction System Symbols Date of manufacturing Manufacturer Catalog number Serial number Alternative current Grounded Conductor HEEE 250v F2A Supplementary Fuse Consult instruction before use Warning Biohazard Risk of personal injury to the operator or a safety Warning hazard to the surrounding area This product is required to comply with the European WEEE symbol Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC bf gt PE taco Nucleic Acid Automatic Extraction System 1 Introduction The taco Nucleic Acid Automatic Extraction System taco extracts nucleic acid by using magnetic separation technology The magnetic beads are modified by coating silica to provide the binding ability to nucleic acid After binding the nucleic acid the beads will be separated from the bio solution The nucleic acid will be purified and eluted by several
9. ocal distributor or GeneReach immediately If any item is damaged do not use the damaged components since the use may lead to poor performance of the instrument 3 1 Safety Information Not following the instructions may cause personal injury or device failure When handling infectious materials always wear a lab coat disposable gloves and protective goggles Do not touch any plug and electric switch with wet hands Make sure to keep the taco away from water Turn off and unplug the machine before moving it Package the taco carefully before moving and delivering it Vibration or crash may lead to damage Place the machine in a room with good ventilation Hold the plug while pulling it out Do not pull the power cord directly Do not place the power cord on any subject with high temperature Do not look straight at the UV light while turning it on Do not repair the machine by yourself including fixing the door and changing the UV Lamp Contact your local distributor or GeneReach directly to deal with the problems Waste disposal must be in compliance with local laws User should revalidate the taco if the device has been moved 3 2 Emergency Stop 3 2 1 Should any emergency occur press PAUSE button or the MAIN SWITCH to stop the taco 3 2 2 After all emergency is cleared please follow the instruction to resume restart the taco See 4 1 amp 4 2 9
10. taco Research Use Only OOOO Nucleic Acid Automatic Extraction System User Manual Manufacturer GeneReach Biotechnology Corporation TEL 886 4 2463 9869 Email sales genereach com No 19 Keyuan 2 Rd Central Taiwan Science Park Taichung City 407 Taiwan Website www tacomag com 2012 02 Content SSMO soccer a E 1 MAVEN ONC COUN o E E uae souseseaveneusicvercusesiaes 2 MN MIRC CO NSC ores ac cotee orto E O O O OE A O O 2 I TCO MEST ICHon PINEDA dag eiioncsouaiasiacuncuocbousiestaguhcobouaseuiaeast 2 PAO Ay OG E E E EE A E E A E A E A E A E 3 SEPO TC OPO WES eea E E A 3 22 acO M SpECICATONS serrian iere E ETEESI E TEE TEREE EAE EENE 3 2 3 Equipments 16 Be Supphed Dy USET sicitin nosswnssscudewedsseseacsnatinrssonunsnissudeamebeyeieatsnatiOesctoseiiediane 3 2A The Pront View piine MCO M arrede Eai en E E EEST EE EEE E 4 2 TNE IRAE VIC WOR ETICO M aan E RE EE a 6 2 0 Loading taco Plate amp SIeeVCeoecsicrsncsoresne unrar enn rer E E aN EEE 7 De MIMO RUAMIE N OOS a E E E E E E E E N 9 JLS M OPMMAUION erkese e E E aE E 9 PE O O A A at ouesediaunernobonsnnedieuss 9 4 Operation Procedure of taco ccccscssssssssssssessssscsesssssssssssssssssesssssssssssssssssssssssssssssssssesssssessseees 10 4 1 How to START the taco o ccecceccessesssesseessessesssecsessnecsecsnecsecsnsesecsnesseeseesnecuecsessneeseeeneeseestes 10 4 2 How to PAUSE the taco cccsccssessssssesseessesssessecsecsnecsecsnesuecsnsssecsseenecsees
11. tions UV Lamp function failure eCheck that the door is closed the UV light cannot operate while the door is open e Check to see if the taco is performing extraction The UV light cannot operate during extraction Contact your local distributor or GeneReach for UV light does not light up assistance Door function failure The door of taco cannot open Contact your local distributor or GeneReach for close normally assistance 62010 GeneReach Biotechnology Corporation All rights reserved For research use only Not intended for any animal or human therapeutic or diagnostic use 15
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